Science Investigatory Project

Annona Squamosa
leaves extract:
A potential dog gel
soap ingredient for
eradication of ticks
Submitted by:
Abenaza, Azzinette M.
Najial, Nicole Jane C.
Permejo, Jearicko E.

Submitted to:
Mr. Oliver Lagman
(Research Adviser)
 Annona Squamosa leaves extract:
A potential dog gel soap ingredient for eradication of ticks

Background of the Study

Materials
The goal for this experiment is to find out
the efficacy of Sugar Apple (Atis) leaves extract so
that it can be used for preventing the ticks and
fleas of the dog.  Leaves extract of 3 Annona Squamosa
We find out too, that our experiment is
not only for dogs but it can be use also for
(Atis)
humans because in this experiment we will come
 2 pieces of a Jar
up the infestation called Pediculosis.
 Alcohol (Green Cross 70% Solution)
 Strainer
 Butter cheesecloth
 Vegetable oil (Hapi Fiesta)
 Dropper
Objectives

This project aims to do an alternative and

effectiveness dog soap in order to prevent ticks
and fleas of a dog so that it would be much
cheaper for some dog owner’s.

After spraying, all of the treatment on the

different containers with the specimen inside, we
started to monitor for the effectiveness of all
treatment by observing dead ticks and fleas, from
the container they have placed it on a clean sheet
of paper then every after observation we put it
back in the container. We have determined that
the ticks and fleas are already dead by observing
its movement and by the use of their bare hands.
Procedure
Wash your hands; soak the leaves to
allow moisture to stay. It is important to pick
leaves that are green and moist to allow for easy
extraction of the extracts. Get a jar put the leaves In Trial 2: Using Licealiz 1 out of 4 ticks are dead,
and pours enough alcohol until it covers it. Cover using our new product which is 25 mL 2 out
so the alcohol does not evaporate and shake it up of 4 ticks are dead and using our new
until couple of days or so. After three to seven product which is 50mL 4 out of 4 ticks are
days, strain the alcohol from the solids and dead.
transfer it to a cover jar. Place the solids back to
the original jar, cover with fresh and put the lid In Trial 3: Using Licealiz 2 out of 4 ticks are dead,
on. After three to seven days strain out the using our new product which is 25 mL 4 out
alcohol as before from the second extraction. of 4 ticks are dead and using our new
Combine it with the alcohol from the first product which is 50mL 4 out of 4 ticks are
extraction and filter it all through butter dead.
cheesecloth to remove any remaining solids. Place
the alcohol in an open container and allow it to Conclusion
evaporate for a few days. After the alcohol
evaporates you’ll be left with the oils. Put the oils We conclude that our new product which
into small bottles with eye dropper cap. is Atis Leaves extract a potential dog gel soap is
more effective in killing ticks unlike to the old
And test the gel soap for its effectiveness product named Licealiz because based on our
in killing the ticks and fleas of a dog by spreading Data and Result our new product have the higher
the gel soap to its whole body, scrubs it for 2-3 number of dead ticks than the Licealiz so that our
minutes and drain/cleanse it. And after that we new product is more cheaper than the other one
will measure the amount of ticks and fleas died by because it is ingredient by Atis Leaves that you
using new product we’ve made and record the can buy in Supermarket or “Palengke” in Filipino.
data in the data notebook.
Recommendation
Data and Result
We recommend our new product to all of you
After 3 Trial 1 Trial 2 Trial 3 because we’ve been prove in our Data and Result
minutes that our new product is more effective than the
Treatment A: 2/4 1/4 2/4 commercial product like Licealiz and it is more
Licealiz cheap because it is ingredient by Annona
Treatment B: 3/4 2/4 4/4 Squamosa or Atis leaves extract and it can be used
Atis Leaves
extract 25 for preventing ticks of the dog that it is the main
mL problem of several Dog’s Owner.
Treatment C: 4/4 4/4 4/4
Atis Leaves
extract 50
mL

This table shows the number of ticks in each trial.

In Trial 1: Using Licealiz 2 out of 4 ticks are dead,

using our new product which is 25 mL 3 out
of 4 ticks are dead and using our new
product which is 50mL 4 out of 4 ticks are
dead.
Documentation
Documentation

Documentation