Chromatographic conditions

A gas chromatographic system equipped

Apparatus :
with flame ionization detector.
DB-5 capillary column (or) equivalent
Column : 30mts X 0.53 mm, 1.5µm

Injection mode : Spitless

Injection temperature : 220°C
Carrier gas : Nitrogen
Flow control mode : Constant Flow
Flow : 3.0mL/min
Rate Temperature Hold Time
(°C/min) (°C) (min)
Oven Program : - 50 5
20 240 16
Detector temperature : 270°C
Makeup gas : Nitrogen
Makeup flow : 25 mL/min
Hydrogen(H2) flow : 30 mL/min
Air flow : 300 mL/min
Injection volume : 2.0 µL