QRT CCRI, Nagpur

For ofcial use
REPORT
of the
QUINQUENNIAL REVIEW TEAM
for the period
01.04.2013 – 31.03.2018
ICAR-National Research Centre for Grapes, Pune

Front page: Variety Manjari Medika released by Centre for juice purpose. A ‘Zero waste’
technology for this variety has been developed.
Back page: Variety Manjari Kishmish released by Centre for raisin making.
REPORT
of the
Quinquennial Review Team

for the period
01.04.2013 to 31.03.2018
for
ICAR-National Research Centre for Grapes
Pune – 412307
Submitted to
Indian Council of Agricultural Research
New Delhi
CONTENTS
Executive Summary ........................................................................................................ 1
1. Introduction ......................................................................................................... 5
1.1 Background .......................................................................................................... 5
1.2 Mandate................................................................................................................ 6
1.3 Organogram ......................................................................................................... 7
1.4 Committees .......................................................................................................... 8
1.5 Scenario of grape industry ................................................................................... 9
2. Process of review ............................................................................................... 11
2.1 Composition of QRT.......................................................................................... 11
2.2 Terms of Reference to QRT ............................................................................... 11
2.3 The Process ........................................................................................................ 12
3. Review ............................................................................................................... 13
3.1 Review of research activities ............................................................................. 13
3.2 Review - Transfer of technology ....................................................................... 58
3.3 Review of Management issues........................................................................... 59
3.4 Comparison of research progress with the previous QRT period ...................... 63
4. Summary of recommendations .......................................................................... 73
4.1 Research ............................................................................................................. 73
4.2 Transfer of Technology...................................................................................... 75
4.3 Linkages ............................................................................................................. 75
4.4 Management ....................................................................................................... 75
Annexure 1 List of projects...................................................................................................... 76
Annexure 2 List of externally funded projects......................................................................... 79
Annexure 3 Publications .......................................................................................................... 80
Annexure 4 Details of Intellectual Property Rights ................................................................. 90
Annexure 5 Awards and recognitions ...................................................................................... 92
Annexure 6 Details of inter-institutional collaborations ........................................................ 102
Annexure 7 Details of field demonstration of technologies................................................... 103
Annexure 8 Training acquired by staff .................................................................................. 104
Annexure 9 List of Scientists on Foreign Deputation ............................................................ 108
Annexure 10 Participation of scientists in seminar/symposia/conferences ........................... 109
Annexure 11 Budget details for five years............................................................................. 114
Annexure 12 Details of revenue generation ........................................................................... 117
Annexure 13 Details of Consultancy Projects ....................................................................... 118
Annexure 14 Contract research trials conducted ................................................................... 122
Annexure 15 Details of training programmes conducted for farmers and stakeholders ........ 135
Executive Summary
The QRT was happy to note that ICAR-National Research Centre for Grapes, Pune has
made commendable progress in many fields and is working in tandem with the needs of the
grape growers and the grape industry. The small team of 16 scientists, ably supported by the
technical, administrative and supporting staff of 22 personnel deserves appreciation for
generating a good name of the institute among the stake holders. Notably the Centre has
developed several agronomic and plant protection technologies for enhancing productivity,
quality and food safety. The observations made by the team and its recommendations are listed
below.
 Production technologies for Manjari Medika and Manjari Kishmish, the varieties developed
at the centre, techniques for improving water use efficiency and the plant protection
schedules evolved by the centre for producing zero residue grapes have been successfully
demonstrated in growers’ field as suggested by the previous QRT.
 During the period of report, 59 accessions were added to the existing germplasm taking the
total collection to 470 accessions. Evaluation of germplasm resulted in the identification of
genotypes with naturally loose bunches and bold berries of more than 18 mm diameter, high
fruitfulness of buds (>3 inflorescences/shoot); and tolerance to major diseases and insect
pests.
 In a survey of the growers’ vineyards in Maharashtra, six mutants from Sonaka and three
from Sharad Seedless were identified. The Centre is assisting the farmers for obtaining
registration of clones under PPVFRA.
 The Centre has made significant contributions in developing new cultivars of grapes for
table and raisin purpose. Two varieties, Manjari Medika and Manjari Kishmish were
released at institute level.
 The hybrid Manjari Medika is released for juice purpose. Its juice, skin and seed were found
to have high antioxidant and anti-carcinogenic properties. Hence its juice could be promoted
as a health drink rather than just a grape juice.
 To ensure availability of Manjari Medika grapes around the year for processing, possibility
of its production under plastic cover and/or double cropping be explored in Tamil Nadu
and around Bengaluru. Manjari Kishmish, the white seedless mutant of Kishmish Rozavis
may be promoted for table grape production also.
 Because of IPR issues, India is not able to introduce patented varieties with desirable traits.
Hence there is a need to intensify conventional breeding at the Centre, for which ICAR may
consider providing additional land, manpower and budget.
 In addition to mutation breeding and directed crossings, selfing and open pollination may
be used in creating segregated population as a means of widening the genetic base.
 So far, good progress has been made in breeding for downy mildew resistance resulting in
planting of sizeable number of hybrids in the field, many of them having good horticultural
traits. Marker for downy mildew resistance has been identified to support and accelerate the
conventional breeding. Attempts should also be made for pyramiding of genes for disease
resistance.
 Besides resistance breeding, emphasis should be given to breeding for naturally loose bunch
and bold berries employing the techniques of embryo culture and marker assisted selection;
and new colour varieties with uniform colour development and colour retention as there is
demand in domestic as well as export market.
Report of the QRT for ICAR-NRCG, Pune (2013-2018) 1

 The Centre has shown very good output in biotechnology work, but the activities are
diversified and not interlinked. Convergence of output of various studies should be taken to
logical conclusion and results from all basic studies should be translated to the applied
aspects.
 Technologies need to be evolved to extend grape cultivation to non-traditional areas,
including off-season production under cover, may be perfected to expand the window in
international market for Indian grapes. A score card for evaluation of non-traditional areas
for suitability to grape cultivation needs to be evolved.
 Technologies development should focus on rationalization of input to reduce the cost of
production, high price realization as well as environment protection with the final aim of
doubling the farmer’s income.
 Nursery activities to produce virus free plants are necessary. Establishment of virus free
scion block of commercially important varieties and rootstocks is necessary. Shoot tip
culture could be a tool in producing virus free stock plants.
 There is no single ideal soil property for grape production per se but rather an ideal set of
soil properties for a given climate, with possible further refinement based on consideration
of target variety and rootstock. Minimum set of soil health indicators needs to be identified
using principal component analysis (PCA) for sustainable vineyards production.
 Threshold levels of tolerance to abiotic stresses by different stionic combinations should be
determined.
 For efficient fertilizer management, there is a need to promote secondary and micro-
nutrients to increase efficiency of major nutrients. Research on micronutrients was on a low
key in the past. It should be initiated and intensified. The relevance of repeated application
of the recommended dose of nutrients year after year needs to be assessed by delineating
the direct, residual and cumulative effect of applied nutrients. Fertigation schedule be
standardized based on the integration of soil and petiole nutrient contents.
 Critical levels of soil moisture or leaf water potential (ψ) at different phenological stages
are needed to be determined to facilitate sensor aided automation of irrigation system.
Deficit irrigation (DI) or Regulated Deficit irrigation (RDI) can also be attempted along with
Partial Root Drying (PRD) technique. They could be more efficient in reducing the water
usage, but the economic impact is reductions in yield/unit area. Hence long term trials to
study the impact on yield and longevity of the vine are necessary in addition to water use
efficiency.
 Sensors may be developed to diagnose the nutrient disorders, moisture stress, and pest and
disease incidence and rectify the same by tailored sprays, as a means of precision viticulture.
 Canopy architecture and training system may be modeled for mechanization of pruning,
while other operations except harvesting are mechanized. Physiological parameters of a
vine, namely the degree of apical dominance, light compensation point, light saturation
point, leaf area contribution for bunch development, spectrum of light for pigment
development in berries and partitioning of metabolites.
 Studies on effect of plastic cover on grapevine growth and productivity showed that
grapevines raised under plastic cover recorded early sprouting, higher yield, early harvest
and reduced incidence of pests and diseases. Efficacy of plastic cover may be evaluated in
preventing berry cracking and rotting under varying intensity and duration of rainfall with
reference to its contribution of soil moisture, relative humidity and turgor pressure in berries
at different stages of their ripening.
2 Report of the QRT for ICAR-NRCG, Pune (2013-2018)

 Research was conducted to standardize cultural practices for certain wine varieties. The
wine grape growers are mostly guided by the foreign consultants engaged by the wineries,
with which they have contract farming. Thus the technologies remain in the shelf of the
Centre.
 Feedback from growers indicated that post-harvest berry drop, development of off flavor
and poor shelf life after certain degree of berry maturity were the deterrents in the
commercialization cultivation of Manjari Naveen. Technologies need to be evolved to
overcome these defects.
 All packages of cultural practices need to be developed by the Centre not only for the
varieties released by the Centre but also Crimson Seedless and Nana purple varieties which
are gaining popularity among growers.
 Guidelines were developed for safe and sustainable grape production based on strategic
research involving the study of fungicide resistance in pathogen, induction of systemic
resistance in vines, use of naturally occurring antagonists in biological control and use of
micro-organisms for faster degradation of pesticide residues; culminating in the production
of “ zero residue grapes” and was acknowledged by grape exporters.
 Further basic studies to elucidate the biochemical basis for resistance and the mode of action
of antagonists may be conducted.
 A survey may be taken up to assess the impact of bacterial leaf spot on yield. Alternate
bactericides including biologicals may be explored for its management.
 The research achievements during the period under report pertaining to suppression of insect
pests in grape eco-system are relevant, encouraging and satisfactory. The basic work on
pheromone has generated interesting information. Research on apneumones
(semiochemicals) concept needs to be exploited for the management of stem borer. Work
on machine learning and artificial intelligence for insect detection and management is a
good initiative.
 Bacterial isolates from the honeydew of mealy bugs for their attraction and effect on
oviposition of the adults of Chrysoperla and other predators in the ecosystem needs
systematic and focused approach to identify volatiles and to plan further applied research.
 Achievement on identification and evaluation of β-glucosidase producing yeast strains and
its impact on wine quality were appreciable. The wines produced by using positive strains
for β- glucosidase were having 13 additional specific aroma compounds.
 The efficacy of β- glucosidase producing yeast strains in increasing the aroma components
needs to be assessed in grapes ripening during different temperature regimes in collaboration
with international research organizations involved in tropical enology. Subsequently
attempts may be made to commercialize these strains.
 Winery by-products namely wine lees and grape pomace powder (WGPP) were used for
enrichment of cookies, sugar free ice cream and yoghurt. The conclusion stating that cookies
prepared by adding coarse pomace performed better in organoleptic test needs to be
reassessed.
 Only about 2.5 per cent of the grapes produced are used for wine making, while 25 per cent
is dried for raisins. Hence there is a need to initiate research on raisin grapes. Since raisin
industry in the country is expanding, the institute should give more emphasis on developing
technologies for grape drying including drying process, packaging, minimizing browning
during storage etc.

 Reduction in drying period by 50 per cent by drying grapes in a chamber 40° C and relative
humidity  15% was a noteworthy finding.
 The Centre has research collaboration in the areas of education, research and infrastructure
facilities with six universities, DBT, two CSIR institutes, 13 ICAR institutes and nine other
organizations.
 It has published 133 research papers, which account for 1.6 papers/scientist /year during the
period under review.
 Scientists of the centre participated in 37 seminars, Symposia and conferences at National
and International level. Sixteen scientists, five administrative and seven technical personnel
participated in HRD and capacity building programmes.
 Extension activities of the Centre must be extended to Karnataka and Tamil Nadu also to
fulfil its national mandate. The Centre should involve ATARI and KVKs located in major
grape growing districts of Maharashtra.
 Additional land is required for establishment of rootstock and scion mother block,
evaluation of hybrids, expansion of buildings in continuum of the existing area. The land
measuring 10 acres transferred to Directorate of Onion and Garlic may be reverted back to
ICAR-NRC for Grapes.
 Scientists and technical staff working in laboratories need more exposure to the state of art
research techniques in plant breeding, bio-fortification, plant nutrition, eco-physiology,
tracer techniques, nano-technology and environment protection.
 There was full utilization of allocated budget, except under the head establishment – salary
and pensions. There has been a substantial increase of 47 per cent in revenue generation.
The increase was mainly due to consultancy services and contractual research.
 QRT felt the need for the following buildings and recommends for their construction
1. A separate building housing plant protection laboratory including pathology and
entomology, one for radio-isotopes and another for post-harvest technology.
2. An auditorium with a capacity of 100-120 persons and a trainees’ hostel.
3. The existing guest house needs to be expanded and better equipped.
4. A common parking facility in the residential area. A demarcated area for indoor
recreational facility for staff members and their families.
 In view of the importance of grape in national economy, farmer’s income and the necessary
intensification of research, it is recommended that the scientific staff strength may be
increased and the Centre upgraded to Institute.
 To increase the visibility at international fora, it is important that Institute actively
participate in the meetings of international bodies. QRT is informed that presently India is
not a member of International Organization of Vine and Wine (OIV), an important
international organization related to grape and grape processing. QRT strongly recommends
that India should become a member of OIV.


1. Introduction
1.1 Background
During 1960-1990, the area under grape cultivation increased in many parts of the
country and India was entering into export market. The growers and exporters faced several
technical problems, which required research to evolve solutions. Owing these problems,
especially with respect to quality grape production to fulfil international market requirements,
representatives of grape growers approached the Indian Council of Agricultural Research to
establish a dedicated research unit for working on grapes to get their problems solved. After
due deliberation on the research needs of grape growers and the grape industry and also to
address the future challenges and opportunities, the Indian Council of Agricultural Research
approved the establishment of National Research Centre for Grapes vide sanction letter No.1
(2)/92-PI&M (Part-III) dated 16 September, 1993.
The then Director General of ICAR, Dr. R. S. Paroda and Deputy Director General of
Horticultural Division, Dr. K. L. Chadha, took keen interest in the establishment of the Centre.
They were ably assisted by Dr. S.P. Ghosh, then Assistant Director General of Horticultural
Division and Dr. S. D. Shikhamany, Head, Division of Fruit Crops, I.I.H.R., Bangalore, and
Officer on Special Duty, National Research Centre for Grapes, Pune.
After due consideration of the soil profile characteristics, climatic factors, proximity to
other research and development institutes and nearness to commercial vineyards, of the land
offered by the states of Maharashtra, Karnataka and Andhra Pradesh; the Committee appointed
by the ICAR selected the land offered by the Government of Maharashtra at Manjari on Pune-
Solapur Road for establishment of the Centre.
The official decision to hand over the land by the Vice-Chancellor; MPKV to DG, ICAR
was announced on 18th January 1997. The selected piece of land belonged to the Maharashtra
State forest department. Mahatma Phule Krishi Vidyapeeth (MPKV), Rahuri, facilitated the
lease by handing over an equal area of land near Nagpur to the Forest department for which,
the ICAR bore the cost of afforestation. The ICAR and NRC for Grapes will be always indebted
to the grape growers, State Department of Agriculture and MPKV, Rahuri for their help for its
initial establishment. The actual land measuring 46.78 ha was leased to ICAR-NRC Grapes on
29th January, 1998. To commemorate its birth, ICAR-NRC Grapes, Pune celebrates 18th of
January as its “Foundation Day”.
The Centre initially started functioning from the newly constructed R & D premises of
the Maharashtra State Grape Growers’ Association (Maharashtra Rajya Draksha Bagaitdar
Sangh - MRDBS) which is adjoining to the site selected and also utilized the newly established
vineyards of MRDBS for carrying out small scale research experiments. However, presently
the Centre functions from Dr G.S. Cheema Bhavan, which houses laboratories and
administrative setup. Besides main building separate buildings for National referral laboratory,
Biotechnology and bio control laboratories are in place.
Dr. S.D. Shikhamany, Principal Scientist, Division of Fruit Crops, I.I.H.R., Bangalore,
was appointed as the Officer on Special Duty on 22nd October 1996. During December 1996,
the first batch of scientific staff was posted to the Centre. In February 1997, recruitment was
made for administrative and technical staff. On 25th February 1997, Dr. S.D. Shikhamany
joined as regular Director of the Centre. He completed his tenure on 25/07/2002 and Dr. P.G.
Adsule took over the reins, first as Director (acting), and then as regular Director on
26/04/2005. After his superannuation Dr. S.D. Sawant took over the charge as Director on

01/06/2013 and continued till 15th March 2019 when he joined as Vice Chancellor of KKV,
Dapoli. At present, Dr Indu S. Sawant holds the charge of Director (Acting).
In May 1997, the first Staff Research Council meeting was conducted under the
Chairmanship of Dr. S. P. Ghosh, DDG (Hort.), ICAR and short-term Research Projects were
formulated to tackle some of the then major problems faced by the grape industry. A group
discussion was arranged with representatives of Maharashtra State Grape Growers Association;
officials of State Horticulture Department; Scientists of MPKV, Rahuri and Agharkar Research
Institute, Pune; to identify and prioritize the research needs of the grape industry.
The first Research Advisory Committee meeting was held in 1998 under chairmanship
of Dr. J.C. Bakhshi, Ex-Vice Chancellor, RAU, Bihar, with Dr. U.V. Sulladhmath, Ex-Prof. of
Horticulture, UAS, Bangalore, Dr. R. Jayarajan, Ex-Prof. of Plant Pathology, TNAU,
Coimbatore, Sh. C. Venkata Reddy, Sh. K. Nageshwara Rao and Sh. V.D. Patil, progressive
grape growers from Andhra Pradesh and Karnataka helped in guiding the research plan. Dr.
Indu S. Sawant was the Member Secretary of this committee. Planting was done in five acre
area to raise experimental plots, while need based research was carried out in growers’
vineyards. Subsequently, many distinguished scientists, such as Dr. K.L. Chadha, Dr. G.L.
Kaul, Dr. S.D. Shikhamany, and Dr. B.M.C. Reddy headed the RAC and gave guidelines for
development of research projects in various aspects of viticulture. Padmashri Dr. K.L. Chadha,
Ex. DDG (HS) is the chairman of present RAC.
1.2 Mandate
 Strategic and applied research on safe grape production and productivity.
 Transfer of technology and capacity building of stakeholders for enhanced and
sustained production of grapes.
 National Referral Laboratory for Food Safety and Pesticide residue in fruits.

1.3 Organogram
QRT
Director IMC
RAC
Research Admn
PME Technical RFD HYPM IRC ITMU Library Farm
Cell laboratories Accounts
AKMU Vineyard care
Research Technology
Estate
HRD review and its sale
PME Professional Publication Nursery
Service and Security
Committee
Functions execution Sale of produce
PG Vigilance
Priority Education
RTIA
setting
Technical IJSC
Project compilation &
evaluation correspondence Public
Grievance
Revenue
generation
WCC
Genetic Resources Production Plant Health Food Comp. OLIC

PHT
and Improvement Technology Management Safety Appln.
Breeding Hort. I Pl. Pathology NRL
Hort.II Entomology
Biotechnology
Pl. Physiology Agril.
Chemistry
Soil Science

1.4 Committees
Research Advisory Committee (RAC)
The following is the composition of present RAC.
1. Padma Shree Dr. K.L. Chadha, Ex DDG (Hort.), ICAR. : Chairman

2. Dr. A.S. Bindra, Former Head, Deptt. of Hort., PAU, : Member
Ludhiana
3. Dr. K.C. Bansal, OSD, NAEP Unit, IARI, New Delhi : Member
4. Dr. Brahma S. Dwivedi, Head, Soil Science and Agricultural : Member
Chemistry, IARI, New Delhi
5. Dr. Abraham Verghese, Ex-Director, ICAR-NBAIR, : Member
Bengaluru and Director, GPS Institute of Agricultural
Management, Bengaluru
6. Dr. Anjan Bhattacharya, Prof. and Head, Dept. of Agril. : Member
Chemicals, BCKV, Nadia, WB
7. Dr. C.K. Narayana, Head, Division of Postharvest : Member
Technology, ICAR-IIHR, Bengaluru
8. Assistant Director General (Hort.-I), ICAR, New Delhi : Ex-Officio Member
9. Mr. Dnyaneshwar Kaule, At Post: Wadhane, Tal. Baramati, : Member
Dist. Pune
10. Mr. Dnyaneshwar Pandurang Dalvi, Kothurne, Post: Pavana : Member
Nagar, Tal. Maval, Dist. Pune
11. Director, ICAR-NRCG, Pune : Ex-Officio Member
12. Dr. Indu S. Sawant, Pr. Scientist, ICAR-NRCG, Pune : Member Secretary
Institute Management Committee (IMC)
The following is the composition of present Institute Management Committee.
1. Dr. S. D. Sawant, Director, ICAR - NRC for Grapes, Pune
2. Director of Horticulture, Govt. of Maharashtra, Pune
3. Commissioner of Horticulture, Government of Karnataka, Bangalore
4. Director of Research, Mahatma Phule Krishi Vidyapeeth, Rahuri
5. Dr. R. G. Somkuwar, Principal Scientist, ICAR – NRC for Grapes, Pune
6. Dr. Anuradha Upadhyay, Principal Scientist, ICAR – NRC for Grapes, Pune
7. Dr. V. Mahajan, Principal Scientist, ICAR-Directorate of Onion and Garlic
Research, Pune
8. Dr. G. K. Mahapatra, Head, ICAR – IARI Regional Centre, Pune
9. The ADG (HS-II), Indian Council of Agricultural Research
10. The AF & AO, ICAR--Central Coastal Agricultural Research Institute, Goa
11. Mr. Dyaneshwar Kaule, Farmers Representative
12. Mr. Dyaneshwar Pandurang Dalvi, Farmers Representative
13. Shri. B. L. Kokkula, Administrative Officer, ICAR - NRC for Grapes, Pune

1.5 Scenario of grape industry
Area under grape cultivation has increased from 44000 ha in 2001-02 to 140000 ha in
2017-18 (Fig. 1). During the same period the production of grape has increased from 1.18
million MT to 2.91 million MT. The major grape growing states in India are Maharashtra,
Karnataka, Tamil Nadu, and Mizoram. About 75% of grape growing area is in Maharashtra.
Grape in India is grown mainly for table purpose. 72% of total produce is used for fresh
consumption, 26% for raisin making and 2% for processing wine and juice.
160 3500
Area (000 ha)
140 3000
120
2500
Production (000 MT)

Area (000 ha)
100
2000
80
1500
60
1000
40
20 500
0 0
Fig. 1. Grape area and production during last seventeen years.

Grape is one of the major fruits exported from India. About 6% of the produce is exported
to Netherlands, UK, USA, Germany, and China. In 2017-18, about 188000 MT grapes with a
value of Rs. 1900 crores were exported.
2012-13 2013-14 2014-15 2015-16 2016-17 2017-18

Quantity (MT) 140967 160256 94377 132648 198471 188221
Value (Rs. crores) 982.04 1437.07 972.77 1362.26 1781.71 1899.95
The export-import scenario during last few years is depicted in figure 2 and 3.

250 2000
1800
200 Quantity Value 1600
Quantity (000 MT)
Value (Rs. crores)

1400
150 1200
1000
100 800
600
50 400
200
0 0
2010-11 2011-12 2012-13 2013-14 2014-15 2015-16 2016-17 2017-18
Export of grapes
Fig. 2. Scenario of grape export

Source: http://agriexchange.apeda.gov.in accessed on 26/12/2018
6000 14000
5000 Quantity (MT) 12000

Quantity (000 MT)
10000
Value (US $)
4000
8000
3000
6000
2000
4000
1000 2000
0 0
2012 2013 2014 2015 2016
Import of grapes
Fig. 3. Scenario of grape import during recent years

Source: http://www.fao.org/faostat/en/#data/TP accessed on 12/02/2019


2. Process of review
2.1 Composition of QRT
The Indian Council of Agricultural Research (ICAR) constituted fourth Quinquennial
Review Team (QRT) for the ICAR-National Research Centre for Grapes, Pune Vide ICAR
Office Order F.No. 1(8)/2018-IA.V dated 11th December 2018 to review the progress of
research done and achievements made during the preceding five year period from 01/04/2013
to 31/03/2018. The composition of the team is as follows.
Dr. S.D. Shikhamany,
Former Vice-Chancellor, Dr. YSR Horticultural University - Chairman
Dr. P.C. Lenka
Former Professor (Hort.), OUAT - Member
Dr. Yadvinder Singh,
Ex-Head, Dept. of Soil Sci., PAU, Ludhiana - Member
Dr. T. Ramesh Babu,
Former Dean of Agriculture, Acharya N.G. Ranga Agricultural University - Member
Dr. R.K. Gupta,
Director, Mahatma Gandhi Institute for Rural Industrialization, Wardha - Member
Dr. Anuradha Upadhyay,
Principal Scientist, ICAR-National Research Centre for Grapes - Member Secretary
2.2 Terms of Reference to QRT
Basically, the idea is to examine whether the research and development programmes are
in conformity with the priorities of the ICAR and the nation.
2.2.1. Research achievements and impact
 To critically examine and identity research achievements of the Institutes, Projects /
KVKs, and their Regional Stations and Sub-stations, AICRPs operated by them vis-
à-vis sect oral programs since the previous QRT and critically evaluate them.
Commensurate with the objectives, mandates and resources of the organization, the
social-economic impact of research on farmers/beneficiaries and transferability of
results to farmers through extension should be critically reviewed.
 The research and its impact should be brought out in quantifiable benchmarks
wherever possible.
 To know the value for money, QRT should assess and bring out the physical outputs
and outcomes vis-à-vis the budget spent during the period under report. If the likely
outcomes are going to take considerable time, the projected outcomes should be
indicated.
 The socio-economic impact of research on farmers / beneficiaries and transferability
of results to farmers being an important aspect of research outcome the transferability
should be mandatory for major research projects.
2.2.2. Research relevance and budget allocation
To examine objectives, scope and relevance of the research programmes and budget of
the Institute for the next 5 years in relation to overall / state / regional / national plans, policies

and long- and short-term priorities and also the Perspective Plan and Vision 2030 and Vision
2050 documents.
2.2.3. Relationship/collaboration with SAUs and other stakeholders
To pinpoint whether the research programmes of the past and proposal for future are in
harmony with the Vision of ICAR (Hq) and the programme of the related Centres of research
and Agricultural Universities, State Government, Private Sector and IARCs.
2.2.4. Linkages with clients/end-users
To examine the kinds of linkage established with the clients and end-users of research
results, i.e. farmers / fishermen and the extent of interest displayed in conducting “on-farm
research”, on farmers’ fields and in organizing demonstrations / training courses for the transfer
of technology to extension agencies and KVKs of the ICAR.
2.2.5. Proposed changes in organization, programmes and budget
To examine whether any changes in the organizational set-up are called for manpower
and funds allocation. The decentralization in day-to-day working and the transparency should
be highlighted. Further, the Committee may also examine the resource generation efforts and
implementation of Project-based Budgeting.
2.2.6. Constraints
To examine constraints hindering the Institute in achieving its objectives and
implementation of its programme and goals and to recommend ways and means of minimizing
or eliminating them.
2.2.7. Looking forward
To look into any other point considered relevant by the Committee or referred to it by
the ICAR, the Institute Director or the Management Committee, in respect of future programme
development, research prioritization and management changes.
2.3 The Process
During the first meeting of the QRT held on 5-6th February 2019, the QRT visited
experimental fields and laboratories. They also deliberated on the Action Taken Report of last
QRT presented by the Director. This was followed by the presentation of research
achievements under different research programme by the programme leaders. A detailed
discussion was held during the presentation to identify the research gaps and thrust areas for
future.
During second meeting held on 11-13th March 2019, the QRT visited processing unit of
Sahyadri Farms in Nasik and plots of the farmers where the institute’s technologies are being
demonstrated. QRT also interacted with the beneficiary farmers to assess the impact of
technologies. On second day of the visit, separate interaction meetings with the Institute
Management Committee, Institute Joint Staff Council and administrative, technical and
supportive staff were held. A discussion meeting with the scientists was held to decide on the
future research programmes. On the third day, interaction with the stakeholders was held.
Representatives of grape grower associations, exporters, officials of state horticulture
department, representatives of raisin and wine industry participated in the meeting.


3. Review
3.1 Review of research activities
3.1.1 Research programmes
During the period of report, the research was carried out under the following broad
research programmes.
1. Conservation, characterization and utilization of grape
2. Genetic improvement of grape
3. Development and refinement of production technologies for enhancing quality,
productivity and sustainability in grape
4. Development and refinement of integrated protection technologies in grape
5. Development of pre- and post-harvest technologies for processing of grapes and
value addition
6. Food safety in grapes and its processed products
Each programme had several short and long duration projects/experiments (List
enclosed in Annexure1).
3.1.2 Externally funded projects
During the period of review, a total of 18 externally funded projects were undertaken.
The externally funded projects were within the mandate of the Centre and many of the cases
part of ongoing institute projects. The details of externally funded projects are given in
Annexure 2.
3.1.3 ATR on previous QRT
Previous QRT report were submitted to the Council on 06/05/2013. Recommendations
were approved in the GB meeting of the ICAR held on 28/06/213. The action taken report
presented during the first meeting is as follows.
Recommendation Action Taken
1. Validation of technology Technologies for water use efficiency have been
developed so far on successfully demonstrated in Jath, Palsi, and Walva
experimental farm of the which are drought prone area in Sangli Dist.
institute and also on the At Jath, a saving of 25.8% and 46.8% irrigation water
farmers’ field. under recommended irrigation schedule and subsurface
irrigation schedule over farmer’s practice was achieved
whereas at Palsi, it was 31.6% and 19.1 % under
recommended irrigation schedule and partial rootzone
drying technique respectively over farmer’s practice.
The same is now being demonstrated in Sawargaon and
MRDBS Farm at Talegaon in Nasik district. A farmers’
vineyard is identified in Jalna for demonstration.
Modalities for collaborative On Farm Trial with KVK,
Jalna are being discussed.
Four promising varieties viz. Manjari Naveen, A18/3,
Manjari Medika and Manjari Kishmish were evaluated
under Pune and Nasik conditions. Optimum bunch load

Recommendation Action Taken
required for obtaining good quality fruit under Pune and
Nasik condition was determined for each variety.
Schedule for the production of Zero Pesticide Residue
grape was demonstrated in four locations.
2. Exploration of additional Exploration was carried out in Srinagar and
gene material from Himalaya surrounding areas from 30th July to 5th August, 2014
/ North Eastern region and and in Leh-Ladakh region during 18-26 September,
also from Western region and 2014 to search for the grapes with loose bunches, bold
their evaluation and berries, disease resistance and black seeded variety for
exploitation. raisin purpose. Seventeen accessions were collected
during the exploration
3. Optimization of nutrient and Nutrient and water requirement of grapevine in heavy
water requirement of soil is optimized. As per NBSSLUP majority of the
grapevine in different soils. soils in Maharashtra range from medium to heavy in
texture.
4. Basic and strategic research A project is underway to develop downy mildew
to be undertaken for downy resistance variety using molecular breeding approach.
mildew resistance in tropical About 275 field planted progenies are being evaluated
grapevines. for their resistance/tolerance to downy mildew
pathogen as well as horticultural traits. Grape genome
sequence is used to identify new molecular markers
linked to resistance QTL RPV3 for their use in selection
of resistant hybrids.
3.1.4 Review of research progress

The Programme wise research achievements and comments of the QRT are given below.
I. Conservation, characterization and utilization of grape
1. Management of grape genetic resources of table, wine, raisin, juice and rootstock
varieties - Phase II
The grape germplasm repository of this Centre has a total collection of 470 accessions.
During the period of report, a total of 59 accessions were added to the germplasm which
included 7 from USDA, USA, 16 from Leh-Ladhakh, 19 wine varieties from France, 11 clonal
selections, 3 from IARI, 1 from Tamil Nadu and 2 from exporters.
The germplasm was screened for commercially important traits like loose bunch,
naturally bold berries, fruitfulness, reaction to diseases and pests and promising genotypes were
identified.
Total 100 grape accessions were characterized based on 35 traits (as per minimal
descriptors of ICAR-NBPGR). Based on the data for two years a grape catalogue was prepared
and published.
During the period of report, 140 grape germplasm genotypes including hybrids and
collections from Leh-Ladhakh were analysed with 8 microsatellite primers and their DNA
profile was generated. Several duplicates were identified. The molecular database was updated
with this data. The molecular data was used to ascertain the genetic identity of several clones
and hybrids. A18/3 was confirmed to be the hybrid of Black Champa X Thompson Seedless

and not Carolina Blackrose x Thompson Seedless as reported earlier. Sudhakar Seedless was
confirmed to be the clone of Thompson Seedless.
Since the grape germplasm block was 18 years old, the trunk and cordon of almost all
the accessions were damaged due to stem borer, dead wood resulting into reduced/no yield.
Hence, the cuttings from each accession were collected and multiplied in polythene bags in the
nursery of this Centre. The rooted cuttings were then planted in the main field for development
of canes. For each accession, three vines were maintained in the field. All the grape accessions
have been grafted on Dogridge rootstock in A2 block.
Multilocation trials were conducted for the hybrids/clones identified by the Centre viz.
Manjari Naveen, Medika, KR White and A-18/3 for three years at different locations
(Dhondgavanwadi, Vinchur, Pimpalgaon (Nashik), Karkamb (Solapur), Horticultural college,
Mandsaur (MP), ICAR-NRC Grapes, Pune and UHS, Bagalkot (Karnataka). Bunch load in
relation to raisin and juice quality was standardized in the mentioned accessions in different
locations.
Based on the data generated through MLT, Medika was released as Manjari Medika for
juice purpose while another accession i.e., KR White was released as Manjari Kishmish for
raisin purpose.
Several of these activities were financially supported by project “Characterization,
regeneration and documentation of grape germplasm” under Consortium Research Platform on
Agrobiodiversity funded by ICAR-NBPGR, New Delhi, during October 2015 – March 2017.
2. Validation of DUS Guidelines for Indian Grapes (Vitis spp.)
This project is funded by Protection of Plant Variety and Farmers’ Right, New Delhi.
The guidelines for DUS testing was finalized during 2013-14 after several task force meeting
since 2012 from the commencement of the project. The technical bulletin entitled
“Characterization of Grape varieties for DUS (Distinctiveness, Uniformity and Stability)” was
released during the training cum awareness program of PPV and FR Act – 2001.
A DUS block was established with 60 reference varieties. Currently the plot is in bearing
age and being continuously used for recording reference varieties data as per guidelines for
grape variety registration purpose.
Grape growers were assisted for filing application for variety registration with Protection
of Plant Variety and Farmers Right Authority (PPV&FRA). The two years of on-site DUS
testing of the candidate varieties viz. Nanasaheb Purple Seedless, New Sonaka, Sarita Seedless,
Jay Seedless, Sudhakar Seedless were completed and the consolidated report of these varieties
were sent to the PPV&FR Authority for further necessary action. Currently four candidate
entries from Kargil, Ladhakh are under on-site DUS testing. These are Bargun, Churgun,
Margun and Rukuchan. First year testing of these varieties is completed.
Three varieties released by the institute viz. Manjari Medika, Manjari Naveen and
Manjari Kishmish are under process of registration.
3. Clonal Selection in Grapes
Survey was conducted in various regions of Maharashtra viz. Sangli, Solapur, Nasik,
Akkalkot and Pune based on the information provided by the grape growers. These vineyards
and vineyards of near-by area were surveyed to identify the vines with economically important
traits such as bold berries, loose bunches and disease resistance, etc. Total nine mutants were
identified from different regions of Maharashtra. Most of the mutants (6) were obtained from
the variety Sonaka followed by Sharad Seedless (3). Mutants from Sharad Seedless particularly

from Boramani area and mutant of Sonaka (Akkalkot area) were recorded with higher bunch
weight, 10 berry weight, berry diameter and berry length as compared to the mother vine. In
case of Walwa area, berry of mutant was more elongated. Mutant vines differed from the
mother vine in terms of berry weight, diameter, length and pedicel thickness. Statistically no
significant difference was observed for bunch weight between mutant and mother vine at
Walwa. At Tuljapur, mutant was found to be superior with respect to the bunch weight because
of higher number of berries in the bunch. At Bhose and Jaysinghpur, Sonaka was cultivated
mainly for raisin purpose without treatment of growth hormones. In both the cases mutants
were found to be higher in range in terms of bunch weight, 10 berry weight, berry diameter and
berry length. These clones are being maintained at the institute. During survey of grape
gardens, growers were educated about the variety registration for superior clones. Currently,
the activity of registration of identified superior clones is undergoing in the DUS project.
QRT Comments
 Available genetic diversity in grape at NRC for Grapes is narrow. Efforts made to
introduce genotypes resulted in a limited success due to many reasons. In addition to
mutation breeding and directed crossings, selfing and open pollination may be used in
creating segregated population as a means of widening the genetic base. The hybrids
and mutants should be characterized in detail and subsequently included in the
germplasm.
 The exploration and collection of germplasm should be a continuous process.
Germplasm should be extensively evaluated to identify the important traits including
resistance to insect pests. Emphasis should be given to identify the genotypes capable
of thriving in conditions arising due to climate change.
 Presently, the germplasm contains only limited number of rootstocks and entire Indian
grape industry rely mainly on Dogridge and to some extent 110R. It is important to
study the root system of other rootstocks and evaluate them for their suitability under
different conditions of soil and biotic stress.
 Some of the well-known indigenous varieties like Anab-e-Shahi, Dilkhush etc. have
several good traits. They should be taken up for registration with PPV&FRA.
 While evaluating germplasm/varieties for raisin, separate score card with weightage for
raisin quality should be used.
II. Genetic improvement of grape
4. Breeding for downy mildew resistance in seedless grape varieties (partially funded
by DBT under the project “An integrated approach of molecular breeding for
downy and powdery mildew resistance in grape”)
During the period of report, a total of 433 inflorescences of cross combination Carolina
Blackrose x Thompson Seedless and Seyve Villard x Thompson Seedless were crossed.
Presently 280 F1 vines are established in the field.
Total 222 F1 progeny were screened in field and in vitro on UPOV rating scale 1-9. Total
16 progenies of Carolina Blackrose (CBR) X Thompson Seedless (TS) and 17 progenies of
Seyve Villard (SV) x Thompson Seedless showed resistance against downy mildew pathogen.
Polygenic inheritance pattern for the downy mildew resistance was observed in progenies of
these two populations.
Ten new markers and three reported markers for RPV3 were screened for their
polymorphism using 10 grape genotypes (Thompson Seedless, Carolina Black Rose, James,

Seyve Villard-18402, Seyve Villard-18315, Seyve Villard-12364, Seyve Villard-12375,
Manjari Naveen, Rizamat, and Seibel). Among the new markers, two markers (VVDM2 and
VVDM5) were found polymorphic. Among the reported markers, three viz. VMC7F2,
UDV737 and UDV305 were polymorphic. 109 hybrids of CBRxTS and 133 hybrids of SVxTS
were analysed with 5 polymorphic markers (UDV305, UDV737, VMC7f2, VVDM2 and
VVDM5). The cosegregation analysis of phenotype and genotype data for RPV3 markers in
both the populations viz. CBRxTS (109 F1 hybrids) and SVxTS (133 F1 hybrids) was
performed. The data indicated all the five microsatellite markers (UDV305_299,
UDV737_279, VMC7f2_206, VVDM2_195, VVDM5_279) showed significant co-
segregation with the downy mildew resistance/susceptibility in the segregating population of
Seyve Villard x Thompson Seedless based on in vitro as well as field screening. Thus these
markers can be used for early selection of resistant progenies. However, no significant
association was observed in Carolina Blackrose x Thompson Seedless population for any of
the markers. Carolina Blackrose is an interspecific hybrid and show consistent resistance
against downy mildew pathogen both in vitro as well as field screening. The inheritance of
downy mildew resistance show double bell shaped pattern instead of a bell shape, a typical
feature of polygenic trait. Phenotyping as well as genotyping data suggests that the resistance
in Carolina Blackrose is due to the presence of a QTL other than RPV3 and needs further in-
depth analysis.
During the grape fruiting season 2017-18 (October 2017 to March 2018), 101 F1 hybrids
belonging to Carolina Blackrose (CBR) x Thompson Seedless (TS) (41) and Seyve Villard x
TS (60) attained fruiting stage for the first time. Observations on 101 F1 hybrids were recorded
for time of bud burst, number of panicle emergence, days for full bloom, days for veraison,
days for maturity and bunch traits like peduncle length (cm), pedicel length (mm), bunch
length (cm), bunch weight (g), berry diameter (mm), berry length (mm), 100 berry weight (g),
bunch compactness (berries/cm), juice percent (%), seeds/berry (no.), titrable acid (g/l), total
soluble solids (TSS) (°Brix). Among the 101 hybrids which attained fruiting stage, 22 were
resistant to downy mildew. 3 hybrid (H103.23, H100.24 and H123.24) were found potential
for table purpose. Based on the pulp content, TSS and acidity, 23 F1 hybrids (11 black and 12
white) were selected for evaluation for raisin purpose. One hybrid (H123.24) showed promise
for black raisins. The results on horticultural traits for promising hybrids will be confirmed in
replicated trials, for which the promising hybrids have been grafted in a separate block.
5. Genetic Improvement of Coloured Grapes
A new breeding project was initiated in 2017-18 with an objective to develop coloured
grape genotypes with better fruit properties like bold berry size, uniform berry colour
development, diverse maturity period etc. Five crosses were developed under this project using
different parent viz., Crimson Seedless, Fantasy Seedless, Christmas Rose, Red Muscat and
Red Globe. Total 300 crossed bunches were harvested out of five crosses i.e. Red Muscat x
Fantasy Seedless (9), Christmas Rose x Fantasy Seedless (10), Red Globe x Fantasy Seedless
(168), Red Globe x Crimson Seedless (62) and Crimson Seedless x Red globe (51).
Establishment of Breeding Block
In order to concentrate the breeding activities of the Centre at single location, a breeding
block is established. 48 grape genotypes selected on the basis of their donor potential have been
planted in this block. Along with the breeding block, a separate evaluation block is also
established to evaluate the crossed progenies obtained from breeding project.

Breaking seed dormancy in Red Globe
The success of germination of seeds of Red Globe, a parent in many of the ongoing
crossing programme, is very low. The efforts were made to break the dormancy of Red Globe
seed wherein scarification with different concentrations of GA3 (1000ppm, 12000ppm and
1400ppm) followed by chilling treatments (with and without sand for 10, 15 and 20 days) were
tried. Seed soaking for two days in GA3 @ 1400 ppm followed by chilling in moist sand at 40
C for 20 days has given maximum seed germination (42%) amongst all treatments.
6. Creating gene and ploidy variations for desired traits in grape using physical and
chemical mutagens
LD25 (0.3%) and LD50 (0.9%) doses of Ethyl Methane Sulfonate (EMS) were determined
based on % survival of stem segments of Thompson Seedless. Similarly, LD25 and LD50 doses
of Gamma rays were also determined. LD25 and LD50 were observed to be 13 Gy and 32 Gy,
respectively. At present 300 mutagenized Thompson Seedless and 50 mutagenized Red Globe
plants are established in the vineyard. To raise more mutagenized plants, 1400 stem cuttings
of Thompson Seedless and Red Globe were treated with gamma rays at BARC, Mumbai and
treated cutting are planted in field.
To induce ploidy using colchicine, protocol for in vitro production of multiple shoots in
Thompson Seedless to determine LD25 and LD50 of colchicine was standardized. MS medium
+ 6.6 µM BAP and MS medium + 4.5 µM BAP were identified for shoot induction (shoot
development in 70% explants). Rooting medium consisting of MS+IBA (2mg/L) induced roots
in 80 % in vitro plants.
7. In silico identification of abiotic stress (salinity) responsive transcription factors and
their cis-regulatory elements in grape
This project was in collaboration with IASRI, New Delhi. Based on in silico analysis of
Expressed Sequence Tags (ESTs) of Vitis vinifera from various databases, a total of 16785 salt
stress ESTs were compiled and assembled using grape genome sequence available in public
domain. The assembled contigs were analysed using online softwares and 110 genes with
conserved domain like DNA binding, different enzymatic functions etc. were identified. Based
on the size of coding region, conserved domain and their role in salinity stress, five genes viz.
F Box TF, bZIP TF, Inositol 3 Phosphate Synthase, APETALLA 2 TF and WRKY TF were
selected. The sequence of 5 selected genes was analysed in five genotypes viz. Thompson
Seedless, Cabernet Sauvignon, Dogridge, Salt Creek and 110R and several point and indel
mutations were identified. The expression of these genes were analysed in leaves of grafted as
well as own root Thompson Seedless subjected to salt stress at different time points.
In treated grafted vines expression of APETALLA transcription factor increased 7 folds
at 15 days whereas it remained unchanged in own rooted vines. Expression of FBOX TF was
up-regulated in grafted vines at 7 days onwards and its expression increased 12 folds at 15
days, whereas in own rooted vines this TF was up-regulated as early as at 24 hr of salinity
stress. bZIP TF was down-regulated in grafted vines at 24 hr whereas in own rooted vines this
TF was up-regulated at 7 days. In grafted vines, expression of WRKY TF was up-regulated at
15 days of salinity stress whereas it remained unaffected in own rooted vines. Expression of
Inositol 3 Phosphate Synthase (I3PS) was up-regulated in own rooted vines at 7 and 15 days
whereas in grafted vines its expression was down-regulated at 48 hr and then remained
unchanged at later stages of salt stress.

8. Proteomic analysis of Thompson Seedless grapes grafted on different rootstocks at
different phenological stages of growth and development
Whole proteome analysis of 5 stages of cluster development in Thompson Seedless
grafted on different rootstocks was carried out using HDMS/MS. Large number of proteins
were detected. Data was analysed to identify differentially expressed proteins. Several
rootstock specific proteins were identified. Several genes were up or down regulated in grafted
vines. Maximum number of genes was affected at veraison stage. Analysis revealed changes
in expression pattern of proteins across different stages of berry development. DE proteins were
assigned to different pathways viz. glycolysis and carbohydrate metabolism, energy
metabolism and photosynthesis, antioxidant enzyme, amino acids metabolism, lipid
metabolism, transport proteins, gene expression and protein synthesis, protein degradation etc.
The rootstock did not have any significant effect on polyphenol and secondary metabolism
related proteins at any development stages of Thompson Seedless.
9. Functional analysis of salt stress response in grapevine
This DBT funded project was in collaboration with National Chemical Laboratory, Pune.
Six months old Thompson Seedless vine raised on own root and grafted on 110R were
subjected to salt stress by irrigating with saline (100 mM NaCl solution) water, whereas control
vines were irrigated with potable water. Among different morphological parameters,
significant differences were observed for plant height, shoot length, internodal distance
between treated and control plants of own root and grafted vines. Effect of salinity stress on
physiological parameters was observed after 13th day of treatment. Accumulation of salt ions
was observed within 48 hr in treated own-root vines as compared to seven days in grafted vines.
Among biochemical parameters, differences in accumulations and/or degradation of organic
acids were observed at different time points in response to salinity stress both in own root and
grafted vines. Also, salt stress did not have significant effect on proline content in grafted
vines, whereas in own-root vines, proline content was increased in treated vines.
Transcriptome analysis was performed by RNA sequencing of root and leaf samples
harvested at 6, 24 and 7 days after treatment. In roots, the expression of a large number of
transcripts were altered in treated samples. At 6h, 453 transcripts were downregulated and 180
transcripts were upregulated. The number of upregulated and downregulated transcripts at 24h
was 381 and 271 respectively. Whereas 131 transcripts were downregulated and 548 were
upregulated at 7 days. In leaf samples, a total of 370 genes with significantly altered expression
in treated vines, were detected in at least one of the three time points. As a group of early
responsive genes a total of 159 genes were up-regulated at 6h. The number of up-regulated
genes was 9 and 211 respectively after 24h and 7d treatment. 21, 46 and 3 genes were down-
regulated at 6h, 24h and 7d respectively. 5 genes were differentially expressed at all the three
time points. The gene enrichment analysis revealed that GO terms related to transcription
factors were over-represented. Salt stress significantly affected several pathways like metabolic
pathways, biosynthesis of secondary metabolites, membrane transport development related
pathways etc. 370 DEGs were distributed on all the 19 chromosomes, however clustered
regions of DEGs were present on chromosomes 2, 5, 6 and 12. These could be probable QTLs
for imparting tolerance to salt and other abiotic stresses. The RNA sequence data was validated
by qPCR. To identify suitable reference genes for qPCR data normalization most stable
reference genes were identified. qPCR of 10 selected genes in control and treated samples of
grafted and own root vines showed that the expression of salt responsive genes is influenced
by rootstock.
The sequence of 10 selected genes was analysed to identify the microsatellite regions.
Primers were designed and screened with fifteen grape genotypes, all the primers were highly

polymorphic. This information will be useful for the identification of key genes involved in
salt stress tolerance in grape. The identified DEGs could also be useful for genome wide
analysis for the identification of polymorphic markers for their subsequent use in molecular
breeding for developing salt tolerant grape genotypes. Based on the expression profiles across
three time points, three genes have been selected for functional validation.
Transcriptome analysis of heat and moisture stress
In another experiment, leaf samples were collected from Fantasy Seedless vines grown
under varying irrigation regime in the morning and at 4.00 PM when the air temperature was
about 40 °C and these samples were used for transcriptome analysis using RNA seq. Analysis
of RNA seq data identified 292 genes as differentially expressed due to deficit irrigation and
1558 differentially expressed genes (DEGs) in response to heat stress. Pathway analysis of
DEGs revealed enrichment of genes involved in protein modification and heat shock proteins.
10. Understanding the rachis and berry elongation in response to GA3 application in
Thompson Seedless grapes using functional genomics approach
This DBT funded project was undertaken in collaboration with National Chemical
Laboratory, Pune. Field grown vines of Thompson Seedless grafted on Dogridge rootstock
were used for GA3 application at different stages of cluster development (rachis, flower cluster
and 3-4 mm berry) and samples were collected at 6, 24 and 48h after application.
Morphological observations (length of rachis, number of rachillae, internodal distance between
two rachillae, berry diameter, berry length and 100 berry weights) were recorded at harvest and
significant differences were observed between treated and control vines. RNA from 6h and 24h
time points from each stage were sequence for whole transcriptome analysis. Transcriptome
analysis of rachis, flower cluster and berry identified large number of up and down-regulated
genes in response to GA3 treatment. 733 genes were differentially expressed in GA3 treated
samples. At rachis and flower cluster stage respectively, 126 and 264 genes were found to be
significantly differentially expressed within 6h of GA3 application. However, at berry stage,
major changes occurred even at 24h and number of DEGs at 6h and 24h were 174 and 191
respectively. Only a few genes were common across all the stages and unique set of
differentially expressed genes (DEGs) were identified at each stage thus suggesting stage-
specific response to the GA3 application. Among the DEGs 67 were transcription factors.
Analysis revealed that several genes belonging to sucrose and hexose metabolism, hormone
and secondary metabolism and abiotic and biotic stimuli, were expressed in higher proportion
in response to application of GA3. The RNA sequence data was validated by qPCR. To identify
suitable reference genes for qPCR data normalization under our experimental conditions,
several reference genes were tested and most stable reference genes were identified. The
expression of 21 randomly selected genes was analyzed in three biological replicates of three
sampling stages at all the time points. Stage-wise and time-point-wise differences in expression
pattern were observed for most of the genes. While expression of some genes was altered only
for a short time, some genes showed altered expression at the later stage also. Chromosomal
localisation of DEGs indicated that DEGs were distributed throughout the genome. The
maximum number of DEGs (69) were placed on LG18, followed by LG1 and LG8.
Genome sequence analysis of 10 selected genes was carried out to identify the
microsatellite regions. The microsatellite primers were used to analyse 24 genotypes with
varying bunch and berry attributes. Seven out of 10 primers were highly polymorphic. These
markers hold promise for their use in markers assisted selection after due validation in a
segregating population for bunch traits. Simultaneously, based on literature and public
databases search homologous grape sequence for 10 GA3 responsive genes from different crops
were selected for expression analysis in experimental samples. Differential expression was

obtained for these genes in control and GA3 treated samples of all stages. Expression of these
genes was also analysed in five grape genotypes such as Manjari Naveen, Red Globe, Fantasy
Seedless, Cabernet Sauvignon and Sauvignon Blanc. Varying expression of these genes was
observed in these genotypes.
The data obtained from whole proteome analysis of rachis, cluster and berries of
Thompson Seedless at different time points after GA3 application was analysed using and
differentially expressed proteins (DEPs) were identified. Pathway analysis revealed enrichment
of pathways related to carbon metabolism, energy related metabolism protein processing
biosynthesis of amino acids and biosynthesis of secondary metabolites etc. Proteome and
transcriptome data was analyzed for correlation between two data sets. Good correlation was
observed for large number of genes and proteins.
QRT Comments
 Success in grape breeding world-over has been through conventional breeding and clonal
selection. The former was by private breeding companies and the latter in public funded
institutions. Conventional breeding must be supported by Biotechnological interventions
such as embryo rescue and Marker Assisted Selection (MAS). It requires huge financial
input, land and skilled manpower, inadequacy of which has been the reason for limited
success. During the period under report, grape industry has been longing for varieties that
have demand in international market. Because of IPR issues, India is not able to introduce
such varieties. Hence there is a need to intensify conventional breeding at the Centre, for
which ICAR may consider providing additional land, manpower and budget.
 Hitherto crosses were made involving varieties with desirable traits and hybrids were
identified and released ascribing and highlighting certain favorable traits; but none of
them could replace the existing varieties commercially. Hence selection of parents should
be made with specific objectives and their progeny needs to be assessed comprehensively
for all useful traits in addition to the trait for which they are bred.
 Emphasis should be given to breeding for naturally loose bunch and bold berries. Under
breeding for uniform colour development, colour retention should be one of the
objectives.
 Good progress has been made in breeding for downy mildew resistance resulting in
planting of sizeable number of hybrids in the field, many of them having good
horticultural traits. However, this work should be further supported by embryo
rescue/culture techniques so that seedless variety can be used as female parent. Attempts
should be made to acquire other sources of resistance either through exploration or from
other countries through NBPGR and incorporate in ongoing breeding with the focus on
gene pyramiding of genes for downy and powdery mildew.
 Rootstock breeding is good initiative and should be intensified.
 Very good output in biotechnology work, but the activities are diversified. Convergence
of output of various studies conducted to identify or resolve a problem is necessary. The
results from biotechnology projects should be taken to logical conclusion and results
from all basic studies should be translated to the applied aspects. The data generated
through RNA sequencing of different abiotic stresses must be analyzed further to identify
the critical proteins which are suppressed during the stress. This information will be
useful for developing the cultural practices to revive the function of these proteins even
under stress.
 Gene editing techniques like CRISPR/CAS should be explored and integrated in ongoing
breeding programs especially for disease resistance breeding.

III. Development and refinement of production technologies for enhancing
quality, productivity and sustainability in grape
11. Performance of wine varieties under Pune condition
The Indo-France collaborative project was initiated during the year 2008-09 with the
objectives to evaluate white and red wine varieties for growth, yield and wine quality under
Pune condition. Ten red wine varieties and nine white wine varieties grafted on 110R rootstock
were planted at a spacing of 8 feet between the rows and 4 feet between the vines.
Syrah, Cinsaut, Caladoc, Grenache Noir, Niellucio, Tempranillo, Petit Verdot, Merlot,
Cabernet Franc and Cabernet Sauvignon were the red wine varieties. Among these Petit Verdot
did not establish under Pune condition. The variety had reduced vigor with very low fruit bud
differentiation. In addition, the varieties Cinsaut and Merlot were poor yielder. The varieties
Cabernet Franc, Cabernet Sauvignon, Caladoc and Cinsaut (152 days) were categorized as
early while Syrah, Grenache, Niellucio, Tempranillo, Petit Verdot, Merlot (161.0 days) were
reported to be late in harvest. The wine prepared from different varieties was subjected to
organoleptic test among twenty different persons. In organoleptic test, the wine made from
Syrah and Caladoc was preferred.
White wine varieties were Viognier, Vermentino, Gros Manseng, Chenin, Muscat,
Sauvignon Blanc, Riesling, Gewurztraminer and Colombard. The following varieties did not
produce vegetative growth and also had poor fruit bud differentiation resulting into reduced or
no bunches. Gros Manseng (5.87 bunches/vine) and Vermentino (17.83 bunches/vine)
produced very less bunches per vine. Based on the duration for harvest, Gewurztraminer (127),
Riesling (127 days) were rated early, Muscat, Sauvignon Blanc and Gros Manseng (132 days
each) as medium and Viognier and Vermentino (142 days each), Chenin White (140 days) as
late varieties.
The wine prepared from different white wine varieties was subjected to organoleptic test.
The wine made from Colombard and Riesling grafted on 110R were preferred with a score of
2.38 and 3.24 score out of 5.0-point scale.
12. Standardization of cultural practices to increase quality yield of wine grape
The project was initiated during 2012-13 with an objective to standardize the cultural
practices to increase the quality yield of wine grapes. Under the project, training system,
planting density and pruning time was standardized.
Standardization of training system to increase wine quality
Cabernet Sauvignon vines grafted on 110R rootstock was trained to three different
training systems (mini Y, Kniffin and VSP). The observations on growth yield and quality
parameters were studied for three years. It was observed that higher pruned biomass of 1.13
kg/vine was recorded in kniffin trained vines followed by Mini Y (0.64 kg/vine) while the
lowest biomass of 0.54 kg/vine was recorded in VSP trained vines. Early bud sprouts after fruit
pruning was achieved in mini Y (9.14 days) than 13.0 days in kniffin trained vines. Yield per
vine was highest in mini Y trained vines (5.63 kg/vine) and the lowest in kniffin trained vines
(3.59 kg/vine). The differences for average bunch weight and fruit quality parameters were
non-significant. Considering the growth and yield parameters, the performance of vines trained
to mini Y trellis was found better.
Standardization of planting density for producing quality wine
Cabernet Sauvignon vines grafted onto 110R rootstock were spaced at 8 feet between
rows while the spacing between the vines was varied from 2 feet, 3-feet and 4-feet thus

accommodating 2722, 1815 and 1361 vines per acre. The data was recorded for bud sprout,
vegetative growth, bunch and berry quality and wine made from different spacing. The bud
sprout was early in 8 X 4 feet spacing (10.0 days) than the delayed sprouting in close spacing
of 8 X 2 feet (13.52 days). Higher TSS of 23.0°Brix was recorded in 8 X 4 feet spaced vines
than the lowest TSS of 20.14°Brix. Higher bunch weight of 97.71 g was recorded in 8 X 4 feet
spaced vines than the lowest of 94.86g in 8 X 2 feet distance. However, number of bunches per
vine were less in 8 X 2 feet spacing (14.86) as compared to 22.57 in 8 X 4 feet spacing
indicating the reduced fruitfulness under dense canopy. Considering bunches per vine, TSS
contents of grape berries and preference of wine through organoleptic test, the spacing of 8 X
4 feet with planting density of 1361 vines/acre was found ideal.
Standardization of pruning time in producing quality wine
Syrah variety grafted on 110R was planted at a spacing of 8 X 4 feet accommodating
1361 vines/acre and trained to mini Y system of training. The vines were pruned on 1st, 10th
and 20th September and maintained by following all the standard recommended cultural
practices. The differences for pruning weight, bud sprout was non-significant. Higher
bunches/vine were recorded in 3rd pruning with an average of 40.20 bunches. The yield per
vine was also higher in third pruning time (4.50 kg) with minimum average bunch weight
(121.86 g). TSS was higher in delayed/late pruning. Juice pH ranged from 3.40 to 3.57. The
wine made from three different pruning time was studied for quality parameters (total acid
(g/l), wine pH, ethanol %, malic acid and volatile acid). However, the differences for these
parameters were non-significant. The wine made from first pruning 1st September was found
better through organoleptic test.
13. Evaluation of rootstock for growth, yield, fruit composition and wine quality of
Cabernet Sauvignon grapes grown in Pune region of India
The Indo-France collaborative project was initiated during the year 2008-09 with the
objectives to evaluate Cabernet Sauvignon on different rootstocks for growth, yield, fruit
composition and wine quality under Pune condition. Cabernet Sauvignon vines grafted on
seven different rootstocks (110R, 101.14Mgt, 140Ru, Gravesac, Fercal, 1103P and SO4) were
received from France and were planted at a spacing of 8 feet between the rows and 4 feet
between the vines and trained to mini Y system of trellis. The observations on growth, yield,
fruit composition and wine quality were recorded during the period of study. The fruits were
harvested after attaining total soluble solids at about 23°Brix. Vine raised on SO4 and Gravesac
were early (136 days) while those on Fercal and 110R were late (144 days). The vines raised
on 110R, 1103P and SO4 were more fruitful with 71.58, 65.41, and 65.25 respectively. Yield
was higher in 110R grafted vines (7.13 kg/vine) followed by Fercal (5.87 kg/vine) and 140 Ru
(4.91 kg/vine). Juice pH was within the range in the fruits of all the rootstocks studied. The
rootstock 110R had less juice potash (885 ppm) compared to other rootstocks. In the
organoleptic test, the rootstocks viz., 110R and 101.14MGT performed better in overall test of
the wine.
Considering the overall performance of Cabernet Sauvignon grafted on different
rootstocks for yield, berry quality, berry biochemical composition and wine quality including
the overall acceptability through organoleptic test, the rootstock 110R and 101.14MGT were
found better.

15. Techniques to improve nutrient use efficiency including farm waste
Direct and residual effects of applied potassium in Cabernet Sauvignon vines grafted on
110R rootstock
An experiment was initiated in 2012 to study the direct and residual effects of potassium
in Cabernet Sauvignon raised on 110R rootstock vines which were fertilised with graded doses
of potassium ( 0 to 600 kg K2O)/ha) during previous growing seasons. The earlier treatments
of 0, 50, 100, 200 kg K2O/ha were retained and these were reverted in case of higher doses of
K, where 200 coincided with 300 kg K2O/ha, 100 for 400 kg K2O/ha and no potassium
application where 500 and 600 kg/ ha K2O was applied. Available potassium (I N Neutral
ammonium acetate extractable) content ranged from 416 to 2740 ppm under different
treatments. All the treatments received 15 ton FYM/ha (on dry weight basis) at the time of fruit
pruning.
In the year 2012-13, yield varied from 17.19 to 19.55 ton /ha and there was no significant
difference in yield, pruned biomass, TSS, acidity and anthocyanin concentration amongst
different treatments. Sodium concentration in berry juice ranged from 69.2 to 55.0 ppm under
different treatments. Potassium content in petioles at bud differentiation stage, full bloom stage
and veraison stage was lowest in control treatment and highest in treatment having highest
available K content in soil. Sodium content in petioles was with in safe limits at all the stages
of sampling. Even in the second consecutive year, no significant response was observed to
applied potassium in terms of yield over control treatments, though highest yield was recorded
in treatment T3 where 100 kg K2O/ha was applied. Further, petiole K content under different
treatments at bud differentiation (0.98 to 1.18%) as well as at full bloom stage (2.60 to 3.06%)
was also above the critical levels of potassium for these stage. Application of manure (FYM)
and/or potassium fertilizer over the years resulted in increase in soil available potassium
(ammonium acetate extractable potassium) in the root zone which was above the established
critical limit of ammonium acetate extractable potassium (375 ppm).
It can thus be concluded that response to various levels of potassium application in terms
of yield, quality and petiole nutrient content cannot be observed if the available soil potassium
levels were higher than 375 ppm for Cabernet Sauvignon raised on 110R rootstock
Diagnosis of leaf reddening symptom in Sharad Seedless grapevine grown under saline
irrigation
The study was conducted on a 12 year old vineyard of Sharad Seedless vines grafted on
Dogridge rootstocks. The vines were drip irrigated and received saline irrigation (EC - 1.70 to
2.01 dS/m and Na - 156.5 to 179.4 mg/l; Cl- - 210.5 to 259.15 mg/l) from different irrigation
sources as per availability of the irrigation water.
The soil analysis carried after the appearance of the symptoms was not conclusive since
apparently healthy and affected vines were randomly distributed. Further the symptoms were
observed on all the shoots of the vine. Hence, tissue analysis was carried out. Leaf blade and
petiole samples belonging to healthy and affected leaves were used for diagnosis of the nutrient
imbalance during the two seasons.
The chlorophyll content of the affected leaves was significantly lower than healthy
leaves. However, the affected leaves had significantly higher chloride (0.61%) contents
compared to apparently healthy leaf lamina. Similar results were observed in the petioles also.
A significant negative correlation was observed between chloride and P concentration in
leaf lamina (r = -0.5034) and petioles (r = -0.7719). Higher chloride content in affected leaves
(lamina and petiole) exhibited a negative correlation with chlorophyll content, but not high

enough to cause necrosis. The chloride content in leaf lamina should be above 1% to cause
toxicity. However, in upper affected leaves where the chlorosis was more severe, the chloride
concentration was below 1%. Even in lamina of lower leaves where the symptoms were less
severe the chloride content was comparatively higher (0.82-1.07%) than upper leaves (0.53 to
0.69%), but still below toxic level. Even petiole content of affected leaves was below excessive
level of 1.5%. This proves that chloride is not responsible for leaf reddening in Sharad Seedless
vines.
Further, there was accumulation of sodium in both leaf lamina as well as in petiole in the
affected leaves as compared to apparently healthy leaves. Even though, calcium, magnesium
and potassium content declined in affected leaf lamina and petioles; nevertheless, highest
decline was observed with respect to potassium content during both the sampling seasons. In
both the sampling seasons, sodium concentration continued to be higher than potassium in
affected leaf lamina whereas in apparently healthy leaf lamina, potassium concentration
remained higher than sodium. In lamina of affected leaves, mean potassium content was
<0.50% during both the pruning seasons whereas sodium content was more than >0.50% in all
the samples ranging from 0.60-0.88%. The potassium content in petioles of affected leaves was
lower than 0.5% in upper leaves during both the pruning season.
Significant and positive correlation between potassium and phosphorus in leaf lamina
(r = 0.8535) and petioles (r = 0.90475) revealed a synergism between the two elements.
Sodium exhibited highest significant negative correlation with K (r = -0.8864) followed by Ca
(r = -0.3332) and Mg (r = -0.3289) in leaf lamina. In petioles also, sodium exhibited highest
significant negative correlation with K (r = -0.6782) followed by Mg (r = -0.3989) and Ca
(r = -0.2658). Thus Dogridge rootstock was not able to restrict sodium accumulation below
toxic range and low potassium levels in leaf lamina and petiole were mainly due to its
substitution by Na+. This study clearly indicates that sodium and chloride accumulation in
vines increases over time and under saline irrigation, nutrient balance should be regularly
monitored in leaf petioles as well as lamina.
Comparison of different sources of organic matter in grapevines including pruned biomass
Farm yard manure was a most common source of manure in the Indian agriculture.
However, its cost has increased due to less availability in the grape growing regions. Based
upon growers demand, an experiment was initiated with an objective to replace FYM either
partly or fully by utilizing the cheaper sources of organic matter such as press mud compost,
green manure and pruned biomass in different treatment combinations. Recommended
fertigation schedule was common across all the treatments.
Significantly highest yield of 19.50 t/ha was recorded in T3 treatment (Press mud) over
T1 (only Fertigation schedule), however, it was on par with other treatments. Amongst the
organic sources, the cost-benefit ratio was highest in the treatment where pressmud only was
used as input (0.611) followed by FYM (0.575). Treatments involving organic sources resulted
in significant increase in petiole N, K, Mg concentration at Full bloom stage and K content at
Fruit bud differentiation. The petiole potassium contents were higher in those treatments where
pressmud compost was used either alone or in combination. The soil organic carbon has
accumulated significantly in the treatments where organic sources have been added over
control. Between the organic sources, pressmud compost alone or in combination have
significantly improved organic carbon over FYM treatment.
Thus, press mud compost alone or in combination can be used as a cheaper source of
organic matter as well as nutrients.

16. Standardizing irrigation schedule in Fantasy Seedless vines based on crop growth
stage and pan evaporation.
The experiment was initiated in 2013 in a 3 year old Fantasy Seedless vines raised on
110R rootstock. Five treatments (irrigation schedule based on crop growth stage and recorded
open pan evaporation) given below were imposed on vines raised under uniform management
conditions. The total pan evaporation and rainfall during period of experimentation ranged
from 1465.53 to 1613.9 mm with rainfall ranging from 519.6 to 566.2 mm.
Irrigation schedule treatments of Fantasy Seedless vines raised on 110R rootstock
Treatments *
Expected duration I II III IV (With V PRD
Growth Stage
(days after pruning) subsurface
irrigation)
Foundation Pruning/ Back Pruning
Shoot growth 1-40 40 30 20 20 20
Fruit bud 41-60
15 15 15 15 15
differentiation
Cane maturity and 61-120
Fruit bud 15 15 15 15 15
development*
121days - fruit 121 -
15 15 0 0 0
pruning *
Fruit Pruning/ Forward Pruning
Shoot growth 1-40 40 30 20 20 20
Bloom to Shatter 41-55 15 15 15 15 15
Berry growth and 56-105 40 30 30 20 30
development
Ripening to Harvest 106- harvest 40 30 20 0 20
Rest period Harvest to - -- -- -- --
Foundation pruning
* Based on per cent replenishment of actual pan evaporation (1mm = 10000 l/ha)
The yield, bunch weight and TSS differed significantly between the treatments.
Treatment T2 on an average utilised 261.5 mm of irrigation water and produced yield
significantly higher than other treatments and on par with T1 treatment that utilised 332.3 mm
of irrigation water. Treatment T3 produced the least grape yield as compared to other
treatments.
Treatment T4 (SS) utilised the least amount of irrigation water of 153.5 mm with yield
(1.7 MT/ha) lower than treatment T2 but irrigation water saving of 108 mm over surface drip
irrigated T2 treatment. This shows the possibility of this treatment being suitable in the areas
where irrigation water availability is less or under drought conditions. However, the WUE was
also least in T4. Treatment T5 (PRD) also produced 2.5 MT/ha less yield as compared to T2
treatment but utilised 59.4 mm less irrigation water. The WUE was also higher as compared
to T2 treatment. Similar trends were recorded across all the years. The leaf water potential (-
bar), proline and phenol content which are indicators of stress differed significantly during
Foundation and Fruit pruning seasons and were significantly lower in T1 followed by T2. Leaf
proline and phenol were also least in T1 and T2 treatments and highest in T3 treatment.

Thus, the surface drip irrigation schedule treatment T2 with 30-15-15-15 per cent
replenishment during Foundation pruning season and 30-15-30-30-0 per cent replenishment
during fruit pruning season has been found to provide yield and quality of the Fantasy Seedless
grapes comparable to treatments with higher irrigation water application.
This variety requires 20% less irrigation water as compared to Thompson Seedless, ruling
variety in the country and hence, could be introduced in areas where less availability of
irrigation water is there. Combining this variety with subsurface irrigation, a water saving
techniques led to 108 mm of irrigation water saving, with only 1.7 MT less productivity than
recommended irrigation schedule.
17. Standardizing Irrigation Schedule for Cabernet Sauvignon vines raised on 110R
rootstock
Six treatments (irrigation schedule based on crop growth stage and recorded open pan
evaporation) given in table below were imposed on vines raised under uniform management
conditions. This schedule was later on modified in the second year after obtaining results of
preliminary trial on withholding irrigation after Fruit pruning. In 2012-13, the total number of
treatments were increased to seven by modifying treatment T6 by withholding irrigation from
91 days after fruit pruning to the harvest stage. The total pan evaporation for the experimental
period for the year 2010-11, 2011-12 and 2012-13 was 1289.19, 1427.27 and 1476.69
respectively.
Irrigation schedule treatments
Growth Stage T1 T2 T3 T4 T5 T6 T 7**
Foundation Pruning/ Back Pruning
Shoot growth (1-40 days) 45* 45 60 30 30 30 30
Fruit bud differentiation (41-60 days) 15 15 15 15 15 15 15
Cane maturity and Fruit bud 15 15 15 15 15 15 15
development (61-120 days)
121days - fruit pruning 30 15 15 15 15 15 15
Fruit Pruning/ Forward Pruning
Shoot growth (1-40 days) 45 45 60 30 30 30 30
Bloom to Shatter (40-55 day) 15 30 15 30 30 30 30
Berry growth and development stage I 45 30 30 30 15 15 15
(56-90 days)
Berry growth and development stage 45 30 30 30 15 15 --
II (91-106 days)
Ripening to Harvest (106-145 days) 45 15 15 15 30 15 --
Rest period (20 days after harvest) 15 - 15 - 15 -- --
* Based on per cent replenishment of actual pan evaporation (1mm = 10000 l/ha)
**Treatment T7 was added in the year 2012
First Year: During the first 75 days after fruit pruning, the vines were not given irrigation
due to frequent rains. The yield under different irrigation schedules ranged from 6.97 to 8.49
t/ha. However, there was no significant difference amongst different treatments. The water use
efficiency however differed and was highest in the treatment T4. This could be due to highest
yield recorded under T4. The irrigation schedule treatment T6 with least irrigation application

of 185.82 mm apart from total rainfall of 594 mm was found sufficient to provide yield and
quality equivalent to other higher irrigation treatments.
Second Year: The vines were not given irrigation due to frequent rains for a total period
of 121 days. The yield under different irrigation schedules ranged from 13.18 to 14.14 t/ha.
However, there was no significant difference amongst different treatments. The treatment T6
with least irrigation input of 196.1 mm plus 533.5 mm of rainfall was found sufficient to
provide yield and quality equivalent to other higher irrigation treatments. The water use
efficiency was also the least in this treatment followed by T4.
Third Year: The experiment was modified by addition of another treatment based upon
last year’s preliminary studies on irrigation withholding after fruit pruning. The yield under
different irrigation treatments ranged from 17.29 to 19.26 t/ha. The irrigation schedule T7 with
least irrigation application of 182.81 mm apart from 192 mm rainfall was found sufficient to
provide yield and quality equivalent to other higher irrigation treatments. The water use
efficiency of 103.42 kg of grapes/mm of irrigation water applied was recorded in irrigation
schedule T7. Analysis of the must for phenols, anthocyanins, tartaric and malic acid did not
show any significant differences between the treatments. The glucose fructose ratio also did
not differ significantly between the treatments. The sodium content in juice and berries were
below the OIV standards of 80 ppm, thereby suggesting that 110R rootstock even after 6 years
of field establishment can exclude sodium and can be safely used under saline irrigation having
high sodium content. There was no significant difference in the vine nutritional status in
response to different irrigation treatments. Soil moisture status below the dripper was least in
case of T7 treatment which differed significantly with other treatments
Thus, the irrigation schedule treatment T7 with least irrigation water application has been
found to provide yield and quality of the Cabernet Sauvignon grapes comparable to treatments
with higher irrigation water application.
18. Developing petiole nutrient guide for Cabernet Sauvignon vines raised on 110R
rootstock
A data set from 96 experimental vineyards and growers vineyards has been added for N,
P and K content for bud differentiation stage and full bloom stage from the vineyards where
yield was not affected by disease or unseasonal rains. Critical levels of N, P and K were
determined at bud differentiation stage and flowering stage based upon scatter plot technique
of Cates and Nelson.
Critical potassium content: Based on four years data set (n=96) generated from
potassium experiment conducted at NRC Grapes, Pune, the critical limit for potassium is 0.70%
for bud differentiation stage whereas it is 0.8% at full bloom stage.
Critical nitrogen content: Critical level for N at bud differentiation stage was found to
be 0.65% whereas at full bloom stage it was 0.75%.
Critical phosphorus content: Critical level for P at bud differentiation stage was found
to be 0.30% whereas at full bloom stage it was 0.35%.
19. Decision Support System for Enhancing Productivity of Grapes under Moisture
and Temperature Stress Conditions (NASF funded)
Grape, although an irrigated crop, has a high risk of yield reduction and even losing the
crop due to extreme weather events. Development of a Decision support system (Web and
mobile based) will improve the farmer’s ability to take crucial management decisions keeping
the economics and long term prospects of the standing crop.

It was a multi institutional project in a public - private partnership mode, funded by
National Agricultural Science Fund. The Institutions involved are NRC Grapes (Lead
institute), IARI (New Delhi) and Shivrai Technologies (Pune). The objectives include
development of data library for crop growth model and decision support system, initiating
grape model development and developing decision support system for improving crop
productivity under moisture and temperature stress conditions.
In a two year study, the effect of moisture stress at different crop growth stages in
Thompson Seedless vines raised on Dogridge rootstock was quantified. The yield loss in
different moisture stress scenarios ranged from 6.4% to as high as 26.2% during the period of
experimentation. Mild water stress (50% of recommended irrigation schedule) during shoot
growth stage coupled with dry period during foundation pruning season caused 26.2% yield
loss worth of Rs. 1,42,750/-.
In the experiment to establish relationship between temperature and phenology of
grapevine, the vines pruned on 1st May, sprouted earlier (12 days) as compared to the vines
pruned on 1st April (16 days). This difference in days taken to sprout in the vines pruned on
1st April could be attributed to low day time RH (< 20%) along with high temperatures between
9 am to 6 pm. In 2013-14, the vines pruned in April and subsequently fruit pruned in October
(3rd and 16th) had significantly higher yield as compared to vines pruned in May and fruit
pruned in Nov. The vines from P4 produced significantly lowest yield. The accumulated
Growing degree days (°C) ranged from 1450.60 to 1464.60 in different pruning dates.
However, the number of days taken to accumulate the growing degree days varied from 117 to
128 under different pruning treatments. During 2014-15, the accumulated Growing degree
days (°C) ranged from 1360 to 1541 in different pruning dates with the number of days ranging
from 112 to 134 under different pruning treatments.
VitisMod, a grape simulation model (beta version) is developed. It is a process based
model designed to run at daily step and it can simulate the growth, development and yield of
grape. It has a modular structure with modules to simulate phenology, canopy, dry matter
partitioning, yield, stress of water and temperature on growth and yield. As of now, the
phenology module is calibrated, validated and evaluated for its simulation efficiency in
farmers’ fields. Using the pehology module, it is demonstrated that the grape phenological
events can be foreacsted with satisfactory level of accuracy (R2 = 0.71-0.88) in the farmers’
fields as indiacted by the evaluation indices.The phenology module is integrated into the web-
based DSS. Apart from this a stand-alone guided user interface (GUI) for VitisMod is
developed. Currently, the phenology forecaster module is enabled. With further calibration and
validation of the other modules (yield simulator, water management simulator and stress
(nutrients, temperature, frost, water logging), the model can be used as a full scale DSS for
grape vineyard management.
Web and mobile based DSS applications was completed and launched. DSS application
handles queries relating to water requirement as per growth stage, nutrition requirement and
problems caused due to heat and moisture stress like leaf curling, leaf drying, leaf blackening,
leaf yellowing and leaf drop, stunted shoot growth, berry discoloration, berry cracking, berry
drop etc. A total of 40 growers were registered and trained on DSS software usage in a
workshop organized at Nasik for validation. Almost 40% of the queries were based upon
irrigation followed by leaf yellowing, nutrition and leaf curling. Software has been enhanced
with new features like irrigation time requirement calculator, nutrient application based upon
soil and petiole test, plot registration on mobile after profile creation on web and optimized
advice based on feedback received from farmers.

20. Use of remote sensing for precision farming – case study for selected grape
vineyards in Nasik (MNCFC funded)
This project was initiated in December, 2015 in collaboration with Mahalanobis National
Crop Forecast Centre (MNCFC) under Dept. Of Agriculture and Cooperation, New Delhi.
Use of ground based hyperspectral data for estimating biophysical and biochemical
parameters
Chlorophyll contents, LAI and hyperspectral data of vegetation were collected during
November 2016 (shoot growth stage), December 2016 (berry growth stage), February 2017
(veraison and maturity) and March 2017 (harvest stage). Correlation analysis was carried out
between the chlorophyll values and magnitudes of hyperspectral vegetation indices and narrow
bands by pooling the data of all 36 points in the farmer field, for each date of observation.
Similar correlation studies were carried out between LAI and respective hyperspectral indices
and band reflectance values. The indices and bands with consistently higher correlation with
chlorophyll content and LAI were chosen as their best indicators.
A negative correlation was found between reflectance values at all bands (hyperspectral
narrow bands) with the chlorophyll contents, since chlorophyll absorbs the radiation, thereby
reducing reflectance. Bands in red region (650-690 nm) had highest negative correlation
followed by red edge bands (695-750 nm), with the chlorophyll content among all the dates, as
compared to other bands.
Leaf area index had higher correlation with four indices viz. Normalized Difference
Vegetation Index (NDVI), Gitelson and Merzlyak 1 (GM1), Lichtenthaler Index 1 (LIC1) and
Structure Intensive Pigment Index (SIPI) among all the dates, compared to other indices. A
negative correlation was found between reflectance values at all bands with the LAI values,
since chlorophyll absorbs the radiation, thereby reducing reflectance. Bands in red region (650-
690 nm) had highest negative correlation followed by red edge bands (695-750 nm), with the
LAI values among all the dates, as compared to other bands.
Assessing and monitoring stresses (especially water stress) in grape using remote sensing.
Moisture stresses is an important abiotic stress affecting grapevine productivity. Between
2012 till 2016, monsoon rains were largely deficient in 2012-13, thereby affecting overall crop
vigour. The temporal vigour of grape classes identified through temporal unsupervised
classification Landsat OLI 8 satellite data of 2012-13, 2013-14, 2014-15 and 2015-16 indicated
that crop vigour in 2012-13 was reduced in contrast to other years, during fruit pruning season.
Use of high resolution remote sensing data for developing management zones in vineyards
In the studies to delineate management zones in terms of soil fertility parameters and its
relationship with yield, 36 soil samples were collected from the plot of 7 acre area. The
vineyard biophysical parameters viz. LAI and chlorophyll content were also recorded from the
vines in the vicinity of the sampled points. The yield of the same vines were also recorded at
the time of harvesting.
For different soil parameters, the CV values ranged from 2.5 to 35.39 % in field 1 and
0.4 to 41.15 % in field 2. In both the fields, P2O5 and K showed highest CV values, indicating
their high variability in the field with reference to their respective mean values. On the other
hand, EC, pH, Cl and Mg were found to be more homogeneous in both the fields, owing to
their low CV (0.4 – 6 %) values. Spatially, very high positive correlation can be observed
between Available N, P and K with the average yield of grapes (kg/vine) and other yield
parameters. In general, a negative correlation can be observed between pH and EC with the

yield parameters. Since organic carbon was more than 0.5 in all the points in both fields, hence
a pronounced correlation was not visible vis-à-vis yield parameters.
Area mapping of grape vines in Nasik district was carried out through single date (20
December 2015) unsupervised classification of Landsat OLI-8 (30 m resolution) imagery. This
classified image shows that grape cultivation is majorly concentrated in four taluks of Nasik
namely- Niphad, Dindori, Nasik, and Chandwad.
Grape phenology map was generated by selecting classes with distinct phenological
cycles among the earlier identified grape classes as per ground truth and temporal profiles. Four
classes were identified according to differences in phenological events, viz. a young grape
vineyard, September, October and November pruned vineyards. Young grape vineyards were
less than 10% whereas September pruned about 5%. Majority of vineyards were October
pruned (55%), while about 30 % of the acreage were pruned during November.
21. Effect of plastic cover on grapevine growth and productivity
This experiment was initiated to study the effect of plastic cover and irrigation levels on
grapevine growth, disease and pest incidence and yield.
The experiment consisted of four treatments on Thompson Seedless vines raised on
Dogridge under uniform management conditions. Two treatments were laid under plastic cover
with recommended irrigation schedule and 80% of the recommended irrigation schedule. Third
treatment was vines under hailnet with recommended irrigation schedule and the fourth
treatment was vines in open with recommended irrigation schedule. As part of salinity
management in the soil, the plastic cover and hailnet were removed during the rainy period
from mid-June to first week of September, 2018. Total pan evaporation and rainfall recorded
during the period was 1579.1 mm and 527.7 mm respectively.
The vineyard experienced rainfall during October, 2017 leading to downy mildew
infestation in the vines in the open and under hailnet. However, the vines under plastic cover
were not affected. Only downy mildew incidences were observed in the shoots of the vines
exposed to the rains. Significant yield differences were observed between the treatments with
the vines under plastic recording significantly higher yield as compared to vines raised in the
open and hailnet. Vines under plastic cover with recommended irrigation recorded significantly
highest yield of 18 t/ha followed by vines with 80% irrigation with 16.6 t/ha as compared to
7.1 t/ha and 3.6 t/ha respectively in vines under hailnet and open conditions. Sugar
accumulation rates in berries (°B) were faster in vines raised in open than the vines under
plastic cover. The time of harvesting was 7 days later in vines under plastic cover than those
in the open.
During Foundation pruning season, sprouting (%) differed significantly between the
treatments with least values recorded under open. The temperature measured using Infrared
thermometer showed significantly higher temperatures under open on both leaves as well as
cordon under open conditions as compared to plastic cover and hailnet treatments. The total
leaf area per shoot was significantly higher under plastic cover and hailnet over the vines grown
in the open during both foundation pruning season and fruit pruning season.
Mealybug, thrips, mite and jassids damage were significantly higher on bunches in vines
raised under open conditions. There was no significant difference for damage by caterpillars in
vines raised under plastic and under open conditions. Powdery mildew damage were
significantly higher on vines raised under plastic and hailnet as compared to open vines.

The samples from various treatments were analysed for all the pesticides of the Annexure
9 of the RMP. In each case, the results were below MRL and the residue values in various
treatments are also quite similar.
22. To demonstrate techniques to improve water use efficiency in growers’ field
Demonstration trials were initiated to demonstrate the effectiveness of the techniques
developed at ICAR-NRC Grapes for improving water use efficiency in Thompson Seedless
vines. Two trials were laid out in farmer’s field at Jath and Palsi in Sangli District. There were
three treatments at Jath namely, Recommended irrigation level based upon crop growth stages
and pan evaporation, subsurface (75% of the recommended irrigation level) and farmer’s
schedule whereas at Palsi, Recommended irrigation level based upon crop growth stages and
pan evaporation, partial root zone drying and farmer’s schedule.
At Jath, during fruit pruning, a total of 10,72,800, 14,94,678 and 20,16,870 litres per acre
of irrigation water was applied under subsurface irrigation treatment, recommended irrigation
level and farmer’s schedule respectively. This resulted in a saving of 25.8% and 46.8%
irrigation water under recommended irrigation schedule and subsurface irrigation schedule
over farmer’s practice. On an average, 12-15 kg per vine was harvested under farmer’s practice
and recommended irrigation level, however, higher yields of 15-20 kg/vine was recorded under
subsurface irrigation. This clearly showed the better utilisation irrigation water over farmer’s
practice.
At Palsi, crop conditions (visually) did not differ between the treatments. The quantum
of irrigation water applied (l/acre) after fruit pruning under recommended irrigation schedule
and partial rootzone drying technique could save 31.6% and 19.1 % respectively over farmer’s
practice. The yield and quality of the produce did not differ between the treatments. This
clearly showed the better utilisation of irrigation water over farmer’s practice.
Subsequently demonstrations were laid at other places also. At Sawargaon (Nasik), the
experiment was laid out in farmer’s plot in Thompson Seedless vines raised on Dogridge
rootstock with four treatments and five replication. The yield and the yield attributes did not
differ significantly between the treatments. The early rains in the 1st fortnight of October, 2017
reduced the bunch numbers per vine. This vitiated the experimental results w.r.t. yield of the
vines. Leaf water potential (-bar) was significantly higher in the treatments T1 and T4 where
more irrigation water was used followed by T2 and then T3 at both 60 and 90 DAP. The
chlorophyll concentration as measured by SPAD showed no significant differences between
the treatments at both 60 and 90 DAP.
At MRDBS plot (Pune), the experiment was laid out in Thompson Seedless vines raised
on Dogridge rootstock with six treatments and four replication from Fruit pruning. The yield,
bunch weight and bunch no. differed significantly between the treatments with significantly
higher values recorded in T1 and T2 treatments. Treatment T2 (Subsurface irrigation) was
significantly superior over other treatments. The leaf water potential, leaf assimilation rates
and leaf proline and phenol content differed significantly among the treatments. The treatment
T2 (subsurface irrigation) was better than other treatments in terms of yield and related
parameters and utilised only 75% of the recommended irrigation schedule.

23. Climate based spatial delimitation of suitable grape growing regions in India using
GIS
A GIS based model was developed to delineate regions with climatic suitability for grape
growing based on climatic data on average monthly rainfall, maximum temperature, minimum
temperature and average annual rainfall. A thematic map was developed on climatic suitability
for grape growing with respect to average monthly rainfall, maximum temperature, minimum
temperature and average annual rainfall using the datasets developed for it. 12 thematic maps
were developed for period wise climatic suitability for grape growing with respect to average
monthly rainfall, maximum temperature, and minimum temperature for 12 periods of five
months duration using the datasets developed for it. Similarly 12 thematic maps were for
climatic constraints for moderately suitable regions for 12 periods of five months duration by
using data sets developed for it.
24. Standardization of bioregulators schedule for improving quality and yield of table
grapes
In Crimson Seedless, application of GA3 (10 ppm) and CPPU (1.0 ppm) at 3-4 mm berry
size and 6-7 mm berry size was found effective in improving the berry size and quality
parameters.
In Fantasy Seedless, application of GA3 (10 ppm) and CPPU (0.5 ppm) at 3-4 mm berry
size and 6-7 mm berry size were effective in increasing the size of the berries and also the
quality of berries.
In Manjari Naveen, application of GA3 (10 ppm) and CPPU (0.5 ppm) at 3-4 mm berry
size and 6-7 mm berry size seems to be effective in increasing berry size as well as quality of
this variety.
In Red Globe, application of GA3 (20 ppm) and CPPU (2 ppm) at 3-4 mm berry size and
6-7 mm berry size were effective in increasing the size of the berries and also the quality of
berries.
Residues analysis of berries at harvest indicated absence of residues of GA3 and CPPU.
25. Physiological disorders and their management in grapes
Sunburn
Different agrochemicals viz. various calcium compounds, silicilic acid, potassium salt of
phosphorus active etc. were applied at 80, 90, 100 and 110 days after pruning on Thompson
Seedless to avoid sunburn. Application of Nutrifight (potassium salt of phosphorus) @ 2g/l
along with paper bag coverage to bunch was found effective to avoid 100% sunburn
particularly in Thompson Seedless grapes.
Use of silixol also reduced the incidence of sun burning in the grapes. Among all
treatments, silixol @ 4 ml/l showed significantly lowest percentage of sunburn followed by
silixol powder @ 2 g/l. In addition to its effect on sun burn, silixol @ 4 ml/l also improved
quality parameters like berry length (18.81 mm) and berry diameter (17.01 mm), TSS and
acidity.
Berry Cracking
The different agro chemicals viz. calcium compounds, silicic acid, potassium salt of
phosphorus active were applied at 74, 84, 100 and 111 days after pruning. The application of
silicic acid @ 16 ppm along with calcium gluconate and vegetable oil 0.3% resulted in reduced
incidence of berry cracking in Fantasy Seedless.

In addition to the incidence of berry cracking, various parameters of berry were also
evaluated. The maximum berry length (22.12 mm) and berry diameter (16.33 mm) were
recorded with the application of silixol @ 4 ml/l. The quality parameters such as TSS and
acidity were greatly influenced by the application of silixol.
Swelling of knot on bunch peduncle
The application of Naphthalene acetic acid (NAA) with different concentrations (40 ppm,
50 ppm and 60 ppm) was performed twice from pre-bloom to flowering. Sodium molybdate
was applied as a foliar spray in different concentrations viz. 0.5 g/l, 1.0 g/l, 1.5 g/l and 2.0 g/l.
An increase in swelling of rachis was observed with the application of NAA @ 60 ppm.
While abnormal phloem cells were observed after 30 days of blooming. The yield and quality
of abnormal grapes were also found inferior in comparison to normal grapes. Foliar spray of
Sodium molybdate @ 0.5 g/l recorded higher reduction of know swelling.
26. Bio-efficacy, phytotoxicity and residue dissipation of Chlormequat Chloride (CCC)
in grapes
Impact of Chlormequat Chloride (CCC) to increase fruitfulness & residue studies in
Thompson seedless grapes
Label claim for CCC includes only one application after fruit pruning. However, recent
discussion with stake holders it was stated that growers need three applications after foundation
pruning, with specific objectives to reduce vegetative growth and to increase fruitfulness
especially under the adverse conditions of high soil moisture after 45 days of pruning.
The experiments were carried out on Thompson Seedless grape variety grafted on
Dogridge rootstock at 4 different locations viz. Pune, Yelavi, Kupwad and Savlaj during the
year 2017-2018. Chlormequat chloride (CCC) was applied in both single and double dose as
foliar spray, where the whole vine was sprayed at different growth stages after back pruning
and forward pruning (in Pune only). The result of this experiment concluded that treatment T-
2 i.e. medium concentration of CCC (1000 ppm, 1500 ppm and 2000 ppm) has highest
numerical inhibitory effect on shoot elongation and inter-nodal distance and have a positive
effect on cane thickness. However it was statistically on par with lower (T1) and higher (T3)
concentration of CCC. The treatment T2 (1000 ppm, 1500 ppm and 2000 ppm conc.) of CCC
reported higher fruitfulness in most of the regions. High concentration of CCC in treatment 3
(1500 ppm, 2000 ppm and 2500 ppm) was reported more residues in berry and dissipation rate
was low as compare to both the treatments.
Residue degradation of CCC through microbial consortium
Different bacterial and fungal consortia in liquid formulation made from isolated
microbes were obtained from National collection of Industrial Micro-organisms CSIR-NCL,
Pune. Phytopathogenic fungal microbial cultures of genera Trichoderma, Pacilliomyces,
Aspergillus, Metarhizium, Beveria, Penicillum and bacterial cultures of genera Bacillus,
Psudomonas etc. were used in Thompson Seedless variety grafted on Dogridge rootstock at
Yelavi, Kupwad and Savlaj region of Sangli district. The fungal and bacterial consortia reduced
the residue level of CCC in grape berries which was below the MRL for European Union.
27. Bio-efficacy of new molecules
During the period, the following commercial formulations were tested for their bio
efficacy to influence the growth, yield and fruit quality.

Plantozyme, a biological derivative from sea algae and herbs, when applied as spray
@ 2.5 ml/l and soil application for granules @ 25 g/vine increased berry length and diameter,
average bunch weight, TSS and yield of Thompson Seedless grapes.
Aura XL, an algal extract protein hydrolysate and chelated micronutrients when sprayed
@ 2.0 – 3.0 g/l, increased leaf chlorophyll, yield quality of Thompson Seedless grapes.
Cabrio Top 60 WG a combination of metiram 55% and 5% pyraclostrobin when applied
@1000-3000 g/ha as a spray, increased leaf size and leaf thickness, yield and quality of
Thompson Seedless grapes.
Cynoboost, containing tetraethyl rhodamine, natural β hydroxytricarballyic acid mixed
with surfactants when used @0.5 ml/l as a swabbing after pruning resulted in uniform sprouting
in Thompson Seedless grapes.
Elanta Super, a derivative of L-cysteine, folic acid with adjuvants when sprayed @0.5 to
2.0 ml/l resulted in increased berry length, diameter, average bunch weight, TSS and yield/vine
in Sonaka grapes.
Ethrel 39 SL a formulation of ethrel 39% SL when sprayed @1500 – 3500 ml/ha resulted
in 5 to 10% leaf fall within 10 days. It resulted in uniform sprouting in Thompson Seedless
grapes.
Application 10 to 50 ppm of ProGibb a GA3 40% formulation increased berry length,
diameter, berry weight, TSS, acidity in Thompson Seedless.
Pinknil (a mixture of abscisic acid (0.5%W/V), cytokinins 0.025%W/V, additives and
diluents) when sprayed @2 ml/l, reduced pink berry incidence in Thompson Seedless.
QRT Comments
 All packages of cultural practices, including suitable rootstock, training system, ideotype
canopy, irrigation and fertigation schedules and use of growth regulators, need to be
developed by the Centre not only for the varieties released by the Centre but also Crimson
Seedless and Nana purple varieties which are gaining popularity among growers.
Feedback from growers indicated that post-harvest berry drop, development of off flavor
and poor shelf life after certain degree of berry maturity were the deterrents in
commercial cultivation of Manjari Naveen. Technologies needs to be evolved to
overcome these defects
 The Centre has released the hybrid Manjari Medika for juice purpose. Its juice, skin and
seed were found to have high antioxidant and anti-carcinogenic properties. Hence its
juice could be promoted as a healthy drink rather than just juice. For availability of its
grape for a longer period in an year and to facilitate the continuous operation of the
processing factory, scope of its double cropping be explored in Tamil Nadu and around
Bengaluru.
 Manjari Kishmish, the white seedless mutant of Kishmish Rozavis, identified by the
Centre is promoted for raisin production. Thompson Seedless under cultivation in India
is a degenerated clone. Grape industry is looking for its original clone for the production
of export quality table grapes. Kishmish Rozavis was not exploited in India because of
its colour. Production of table grapes from its white mutant, Manjari Kishmish may be
attempted taking the example of producing export quality table grapes from 2A clone, a
mutant of Thompson Seedless, identified at UC Davis, USA for raisin production.
 Observations of the Pathologist of the Centre are that Leaf roll virus is widely prevalent
in India. Nursery activities to produce virus free plants are necessary. Establishment of

virus free scion block of commercially important varieties and rootstocks is necessary.
Faster and economical method of production of pre-grafted plants of desired
combinations could be better option than tissue culture production of rootstock plants,
the protocols of which are already available at ICAR-IIHR. Infrastructures like soil
sterilization unit, net house, poly house and nursery sheds should be developed. The
technical staffs should be trained on quality planting materials on commercial basis.
 Research was conducted to standardize cultural practices for certain wine varieties. The
wine grape growers are mostly guided by the foreign consultants engaged by the
wineries, with which they have contract farming. Thus the technologies remain in the
shelf of the Centre. Only about 2.5 per cent of the grapes produced are used for wine
making, while 25 per cent is dried for raisins. Hence there is a need to intensify research
on raisin grape production and processing technologies to improve the hygiene and
storability should be intensified.
 There is a need to determine the ideotype architecture of vine of different cultivars of
grape. This is the basis for spacing and training of vines. Cane being the unit of
production, its optimum thickness, length and number/vine need to be determined. The
degree of apical dominance helps determine cordon length and cane number. Leaf area
requirement and optimum LAI need to be standardized. Parasite leaves have to be
identified and removed based on the light compensation point in a given training system
to manage the canopy microclimate.
 The fraction of light in the visible spectrum associated with the development of
anthocyanins and/or carotenoid pigments of a given variety, needs to be identified and
practices standardized for the development of colour, specific to the variety.
 The practice of removing the unproductive shoots during fruiting season is a common
practice. Two questions arise from this practice. i) whether the non-bearing shoot on a
same cane or one on a different cane on the same cordon would not nourish the cluster
on a bearing shoot?; ii) whether nonbearing shoots not enrich the permanent structure
(Cordons and stem) with carbohydrates?. Partitioning of metabolites with C14 needs to
be studied to resolve the same. Study of source-sink relationship at different phonological
stages is necessary to identify the stage of removal of unwanted growth, if necessary.
 The basis for preferential absorption of monovalent and divalent cations is the root CEC.
The demarcating levels of root CEC may be determined for such absorption in important
rootstocks.
 Any future studies that establish stable relationships between vegetation spectral
responses and fruit quality will be of interest. Growth/yield limiting nutrient(s) may be
identified and guidelines developed for optimum use of nutrients by modern methods of
interpretation of leaf analysis data such as Nutrient Ratio, Compositional Nutritional
Diagnosis (CND) and Principal Component Analysis (PCA).
 For efficient fertilizer management, there is a need to promote secondary and
micronutrients to increase efficiency of major nutrients. Research on micronutrients was
on a low key in the past. Micronutrient research, with particular reference to nano
particles and enzyme activity as the indicator of their status in vines, needs to be initiated.
 Deficit irrigation (DI) or Regulated Deficit irrigation (RDI) can also be attempted along
with Partial Root Drying (PRD) technique. They could be more efficient in reducing the
water usage, but the economic impact is reductions in yield/unit area. Hence long term

trials to study the impact on yield and longevity of the vine are necessary in addition to
water use efficiency.
 Emphasis should be on precision viticulture. For this, it is important to strengthen
information on the spatial variability of soil fertility and develop soil fertility maps of
grape growing regions. If much variation is recorded across regions, then region specific
recommendations may be considered. Critical levels of soil moisture or leaf water
potential (ψ) at different phonological stages are needed to be determined to facilitate
sensor aided automation of irrigation system. Sensors may be developed to diagnose the
nutrient disorders, moisture stress, and pest and disease incidence and rectify the same
by tailored sprays, as a means of precision viticulture.
 A score card for evaluation of non-traditional areas for suitability to grape cultivation
needs to be evolved. There is no single ideal soil property for grape production per se but
rather an ideal set of soil properties for a given climate, with possible further refinement
based on consideration of target variety and rootstock. Minimum set of soil health
indicators needs to be identified using principal component analysis (PCA) for
sustainable vineyards production.
 The new and simpler techniques like apparent soil electrical conductivity (ECa) and Vis-
NIR field spectroscopy may be tried for in situ analysis of soil health parameters, and
carbon, N and moisture contents; and the standards of parameters established. Influence
of organic manuring on soil microbial load as an indicator of soil health status may be
studied.
 Rootstock suitability to be determined based on the purpose of cultivation, expected yield
and quality and soil condition. Superiority of 110R over Dogridge and 1613C for abiotic
stress tolerance and yield and quality of table grapes to be confirmed in multi-location
trials. Threshold levels of tolerance to abiotic stresses by different stionic combinations
should be determined.
 While identifying the grape growing regions across countries using GIS tools, if
available, data on relative humidity and sunshine hour may be included as parameters for
delineation of areas.
 Efficacy of plastic cover in preventing berry cracking and rotting under varying intensity
and duration of rainfall with reference to its contribution of soil moisture, relative
humidity and turgor pressure in berries at different stages of their ripening.
 Physiological parameters like leaf area index, light interception under different training
systems may be recorded in all the experiments on canopy management.
 Technologies development should focus on rationalization of input to reduce the cost of
production, high price realization as well as environment protection with the final aim of
doubling the farmer’s income.

IV. Development and refinement of integrated protection technologies in grape
28. Monitoring of fungicide resistance in natural field populations of Plasmopara
viticola and Erysiphe necator in commercial grape vineyards and developing
mitigating strategies (Extramural)
Fungicide resistance in Plasmopara viticola
The present study was undertaken to check for the presence of QoI resistance in field
isolates P. viticola. During, October to December 2014, fifteen downy mildew affected samples
were collected from seven vineyards in Maharashtra. One sample was also collected from
Mizoram state in north India, where there is no history of QoI fungicide use. The EC50 of 5
isolates collected from vineyards with no QoI application during the season was ≤ 0.13 µg/ml
to azoxystrobin and 0.03 µg/ml to kresoxim methyl, whereas the EC50 of 11 isolates from
vineyards where 7-11 applications of QoI fungicides were made during the season was ≥ 110
µg/ml. In the QoI insensitive isolates, the nested PCR product of 332 bp was digested by
restriction enzyme Fnu4HI in two products of 134 and 198 bp confirming resistance due to
G143A point mutation. This was the first report of presence of QoI resistance in P. viticola
from India.
Similarly study was conducted to detect the possible presence of CAA resistance in P.
viticola isolates from commercial vineyards of Maharashtra using biological and molecular
methods. The P. viticola isolate from this Centre recorded less than 0.1 µg/mL EC50 values for
both dimethomorph and mandipropamid showing its sensitivity to CAA fungicides. The EC50
values of the 4 isolates from the commercial vineyards were 54.28 to >100 for dimethomorph
and 42.54 to >100 for mandipropamid showing their insensitivity to the CAA fungicides.
Furthermore, the 144 bp PCR product of PvCesA3 gene was not cleaved by AluI restriction
enzyme in the sensitive isolate from this Centre, while it was cleaved into a 107 bp and a 37 bp
product in the insensitive isolates confirming development of resistance.
Fungicide resistance in Erysiphe necator
Twenty nine E. necator isolates were collected from different geographical regions of India.
In leaf disc bioassay using azoxystrobin, the EC50 of four isolates from research farms was <1
µg/ml, while 25 isolates from commercial vineyards had EC50 more than 115 µg/ml. The 256 fold
resistance factor indicated G143A mutation. All the resistant isolates produced a 100 bp PCR
product with G143A mutant allele specific primer which was not produced by the four sensitive
isolates. A primer pair was designed for partial amplification of cytochrome b gene (Cyt b) and
used for amplification of the gene from two resistant and two sensitive isolates. Alignment of
amino acid sequences showed that the QoI resistant isolates harboured a G143A mutation, which
was absent in the sensitive isolates. The two haplotypes of Cyt b gene from a resistant isolate,
SAA2, and a sensitive isolate, HP1, have been deposited in GenBank under accession numbers
KY418049 and KY418048 respectively. This is the first report of presence of QoI resistant
isolates of E. necator from India.
Fungicides of demethylation inhibitor (DMI) group are used worldwide for the
management of Erysiphe necator but are associated with medium to high risk of development of
resistance in the pathogen. In this study, 54 E. necator isolates were collected from vineyards
located in different geographical regions of India. Four isolates were sensitive (MIC ≤ 1.0 µg/ml),
nine were moderately resistant (MIC 1.0 - <10 µg/ml) and 41 were resistant (MIC > 10 µg/ml)
to the fungicide myclobutanil. The resistance factor (RF) ranged from 1.5 to 295. In PCR
amplification of a specific allele, the product specific for A495T mutation was produced only in
the 43 isolates with RF > 4. The CYP51 gene sequence analysis confirmed A495T mutation
leading to Y136F change associated with high levels of resistance to DMI fungicides. Cross

resistance studies among the DMI fungicides showed that 11 out of 13 myclobutanil resistant
isolates were also resistant to difenoconazole and tetraconazole. Three myclobutanil sensitive
isolates were also sensitive to difenoconazole and tetraconazole. Detection of resistance in E.
necator isolates from the major grape growing region of tropical India stresses on the need for
developing resistance management strategies.
Isolation and evaluation of naturally occurring antagonists of P. viticola
Five mycoparasitic fungi were isolated from sporangiophores of Plasmopara viticola
collected from vineyards of five grape growing regions in India. Four isolates were obtained
from the P. viticola growth on leaf (M1, M2, M10, and M12_1) and one from growth on berry
(M12_2). Morphological observations showed that all isolates belonged to the genus Fusarium.
Phylogenetic analysis of ITS and tef 1α gene identified them as F. delphinoides (M1, MCC
1343), F. brachygibbosum (M2, MCC 1344), F. pseudonygamai (M10, MCC 1345 and M12_1,
MCC 1346) and a Fusarium sp. nov. (M12_2, MCC 1347). In the leaf disc assay all isolates
showed Fusarium species coiling around sporangiophores of P. viticola and inducing lysis.
They also inhibited sporangia production. To the best of our knowledge this is the first report
of Fusarium species as putative mycoparasites of P. viticola in vineyards of India.
These five Fusarium species viz. Fusarium delphinoides (MCC 1343), F.
brachygibbosum (MCC 1344), F. pseudonygamai (MCC 1345, MCC 1346) and a Fusarium
sp. (MCC 1347) were used for bio-control studies. Pre-treatment of leaf discs with Fusarium
species reduced sporangial production of P. viticola by 60 to 75%. Extracellular lytic enzymes
screening showed that all five species produced glucanase, chitinase and protease enzymes. In
the small scale field trial the five species significantly reduced downy mildew incidence on
leaves and bunch as compared to untreated control. The area under disease progress curve
(AUDPC) ranged from 199.25 to 212.41 for leaves and 29.69 to 55.79 for bunch in Fusarium
treated vines. The AUDPC in untreated control was 331.47 on leaves and 185.32 on bunch.
These Fusarium species show potential for biological control of downy mildew of grapes.
Naturally occurring antagonists of Erysiphe necator
A study was conducted to identify potential antagonists which can be used solo or in
integration with safer chemical. Three mycoparasitic fungi were isolated and evaluated for
parasitism and biocontrol. Light and scanning electron microscopic analysis showed that the
hyphae of mycoparasites grew over the powdery mildew colony forming a mycelial web over
it, coiled around the conidiophores and conidia of E. necator, penetrated the conidia and caused
their total collapse. Based on molecular identification the mycoparasites were identified as
Lecanicillium antillanum, Acremonium sclerotigenum and Sarocladium terricola. All three
isolates were positive for production of β-1-3 glucanase, cellulase, chitinase, protease, amylase
and lipase which are involved in bio-control mechanism. During pot, nursery and vineyard
trials all the isolates consistently showed powdery mildew reduction and achieved the 41.76 %
to 65.61 % disease reduction. Sarocladium terricola was more effective in all the treatments.
All three mycoparasites were compatible with chitosan and sulfur and alternate applications of
mycoparasites with these two safe chemicals, the efficiency of L. antillanum, A. sclerotigenum
and S. terricola was increased by 20.16, 27.33, and 8.94 % respectively on leaves and 20.85,
21.36, and 16.06 % respectively on bunches as compared to their solo applications. The study
introduces new possibilities for control of grape powdery mildew using safer alternatives.
Genetic variation and survival of Erysiphe necator in tropical India
Two genetic groups, A and B, have been described for Erysiphe necator which
overwinter as flag shoots or chasmothecia respectively. A population genetic analysis of 26
and 134 E. necator isolates from temperate and tropical regions of India respectively was

performed. PCR analysis showed presence of both genetic groups A and B in temperate regions
and only genetic group B in tropical regions. Flag shoots were also not observed in tropical
region. Weather data analysis and temperature sensitivity of E. necatorconidia indicated
possibility of over-summering of pathogen as conidia on bark. Sequence analysis of ITS
(Internal Transcribed Spacer), IGS (Intergenic Spacer), TUB2 (Beta-Tubulin) and EF1-α
(Translation Elongation Factor 1-α) genes covering 1762 nucleotides from 22 isolates showed
presence of four haplotypes, A-IN1, B-IN1, B-IN2 and B-IN3 in temperate regions and only
haplotype B-IN3 in tropical regions. Haplotypes A-IN1 and B-IN1 were similar to haplotypes
of group A and B respectively reported from USA, Europe and Australia. The other two
haplotypes, B-IN2 and B-IN3 are new reports from India and grouped with haplotypes from
USA.
29. AMAAS projects: Isolation, characterization, bio-efficacy evaluation and
formulation of viticulturally important micro-organisms (01/01/2006 to 31/03/2014),
Development of microbial formulations for biological control of grape diseases
(01/04/2014 to 31/03/2017), Bio-intensive disease and pest management module for
production of residue compliant quality grapes (01/04/2017 to 31/03/2020)
Biodegradation of pesticide residues on grapes
In this study, bio-augmentation of the grape fructosphere was attempted as a novel
approach for enhancing the degradation rate and reducing the residues on grape berries to safe
levels. Four Bacillus subtilis group strains viz. DR-39, CS-126, TL-171 and TS-204 isolated
from grapevines or grape rhizosphere, and earlier identified for their ability to control powdery
mildew disease, were evaluated for their bio-degradation potential of pesticides frequently
detected at harvest, viz. profenofos, carbendazim, myclobutanil, tetraconazole and flusilazole.
Bio-degradation of profenofos
The biodegradation of profenofos was studied in liquid culture, on grape berries and in
vineyard soil. Each one of the four Bacillus enhanced the degradation of profenofos in all three
matrices. Degradation rate constants were best obtained by first + first order kinetics module.
In nutrient broth spiked with 5 µg/mL profenofos, inoculation with Bacillus strains DR-39, CS-
126, TL-171 and TS-204 reduced the half-life (DT50) of profenofos to 4.03, 3.57, 2.87, 2.53
days respectively from the 12.90 days DT50 observed in the uninoculated control. In Thompson
Seedless grapes sprayed with profenofos at field dose of 1,250 mL a.i./ha, the DT50 values were
1.07, 1.00, 2.13 and 2.20 days in grapes inoculated with Bacillus strains DR-39, CS-126, TL-
171 and TS-204 respectively as compared to2.20 days in uninoculated grapes. These four
Bacillus strains also enhanced the degradation of profenofos in autoclaved soil (5.93, 7.47,
6.00, 4.37 days DT50) and in non-autoclaved soil (0.87, 2.00, 2.07, 2.43 days DT50) amended
with 5 µg/g profenofos from the half-live of 17.37 and 14.37 days in respective uninoculated
soils. Growth dynamic studies indicated that all the four strains were able to establish and
proliferate on berries and soil equally well in presence or absence of profenofos. Degradation
product 4-bromo-2-chlorophenol was identified by GC-MS. Strain DR-39 was most effective
in natural environments of grape and soil.
Bio-degradation of carbendazim
In liquid medium, each of the four strains could utilize carbendazim as the sole carbon
source. The half-life was minimized from 8.4 days in the uninoculated spiked control to 4.0 -
6.2 days by the four strains. In Thompson Seedless sprayed with carbendazim at 1.0 g L-1, the
residue on grape berries in control was 0.44 mg/kg after 25 days of application, whereas in
grapes treated with the four Bacillus strains, the residues had decreased to 0.02 mg/kg. The
degradation kinetics showed low half-lives of 3.1 to 5.2 days in treated grapes as compared to

8.8 days in control. In inoculated soils, the half-lives were 5.9 to 7.6 days in autoclaved and
6.5 to 7.2 days in non-autoclaved soils as compared to 8.2 and 8.0 days in respective controls.
The growth dynamics of these strains in all the three matrices was not affected by presence of
carbendazim. Bacillus strains TS-204 and TL-171 showed higher degradation rate than the
other two strains in all the three matrices and show promise for in situ biodegradation of
carbendazim.
Bio-degradation of triazole fungicides
The Bacillus strains utilized three triazole fungicides, myclobutanil, tetraconazole and
flusilazole, as sole carbon source and enhanced their in vitro rate of degradation. Myclobutanil,
tetraconazole and flusilazole were applied in separate Thompson Seedless plots at the field
doses of 0.40 g/L, 0.75 mL/L, and 0.125 mL/L, respectively. Residue analysis of field samples
from the treated fields reflected 87.38 and >99 % degradations of myclobutanil and
tetraconazole, respectively, by the strain DR-39, and 90.82 % degradation of flusilazole by the
strain CS-126 after 15-20 days of treatment. In the respective controls, the corresponding per
cent degradations were 72.07, 58.88 and 54.28 respectively. These Bacillus strains could also
simultaneously degrade the residues of profenofos, carbendazim and tetraconazole on the grape
berries and can be useful in multi-class pesticide residue bio-remediation.
Developing a viable formulation of Bacillus subtilis-DR-39
A commercial product of B. subtilis strain DR-39 was developed in collaboration with
M/s Zytex Biotech Pvt. Ltd., Mumbai (Collaborative partner for commercialization of the
strain). The technology for commercial scale multiplication of B. subtilis DR-39 was developed
and it was formulated as a Wettable Powder (WP) containing 1 x 109 cfu/g formulation with
required shelf-life.
Evaluation of the WP formulation of Bacillus subtilis-DR-39
Dose optimization: The trial was conducted for optimizing the dose of B. subtilis DR-39
formulation based on the dissipation of eight pesticides. The average enhancement in
dissipation by B. subtilis DR-39 was 13.7 % @ 1.0 g/l, 19.0 % @ 2.5 g/l and 22.7% @ 5.0 g/l.
Hence, the middle dose of 2.5 g/l was selected as the optimum dose for further studies.
Bio-efficacy: Application of B. subtilis DR-39 @ 2.5 g/l enhanced pesticide degradation
rate of all studied pesticides on grape berries. The half-lives were reduced by 6 days for
hexaconazole, 3 days for emamectin benzoate, 2.5 days for dimethomorph and tetraconazole,
by 1.5 days for myclobutanil and thiophanate methyl, and by 1 day for carbendazim and
buprofezin. Studies have shown that B. subtilis DR-39 applications in vineyards can be utilized
for faster degradation of multi-class pesticide residues.
Induction of systemic resistance in grapevines
With Trichoderma species
Thirty four Trichoderma isolates were systematically screened for induction of systemic
resistance in grapevines against powdery mildew disease. Trichoderma 5R, NAIMCC-F-
01769, NAIMCC-F-01812 and NAIMCC-F-01951 were selected for field trials based on initial
screening on potted plants. All the four isolates tested positive for production of plant growth
promoting bio-chemicals, indole acetic acid, siderophore, ammnia, hydrogen cyanide and
phosphate solubilization and produced chitinase, β-1,3-glucanase, cellulase, amylase and
protease. These four isolates were compatible to each other and were tested singly or as
mixtures. In the three field trials the maximum reduction in powdery mildew severity was
found in vines treated with 5R + NAIMCC-F-01812, and then in vines treated with 5R alone
showing that the efficacy of 5R is slightly enhanced when used along with NAIMCC-F-01812.

Total phenol contents, and chitinase and β-1, 3-glucanase activities were highest in leaves of
vines treated with 5R + F-01812 corroborating the field observations. The peroxidase and
polyphenol oxidase activities were also high in this treatment. Multilocus analysis using partial
nucleotide sequences of act, rpb2 and tef1 genes showed phylogenetic affiliations of all the
four isolates to T. asperelloides.
With Bacillus spp.
Two hundred and ninety three bacteria from the grapevine ecosystem were screened in
vitro for their growth promoting activities. The twenty two positive isolates, identified as
Bacillus species were further screened in vivo for their ability to reduce disease severity and
eleven promising isolates which reduced disease severity by 35 per cent or more were
identified. These Bacillus species induced higher levels of peroxidase, polyphenol oxidase,
total phenols and total proteins in leaves of treated plants and also tested positive for IAA, NH₃,
HCN and siderophore production, which are implicated in plant growth promotion. The most
promising of these, TS-45, DR-92, TL-171, and TP-232 were evaluated, alone or in binary
combination, on field grown grapevines. All treatments reduced the powdery mildew severity
as compared to the control in all the three trials. Combined applications, of TP-232+TL-171
and TS-45+DR-92 were more effective. Treated vines showed higher level of chitinase, 1,3-
glucanase, peroxidase and polyphenol oxidase activities. In morphological and 16S rDNA
gene analysis, isolates TS-45, DR-92, TL-171, and TP-232 showed close homology to Bacillus
subtilis, B. endophyticus, B. licheniformis and B. flexus respectively.
Biological control of grape diseases
Using Trichoderma species
Field trials were conducted to systematically screen six potential Trichoderma isolates
for control of powdery mildew disease. Treatments were applied as foliar sprays with a simple
liquid formulation of Trichoderma containing 5 x 106 spores ml-1. Isolates NAIMCC-F-01938
and NAIMCC-F-01965 were superior to the other four Trichoderma isolates reducing disease
by 43.67 – 50.36% and 35.71 – 53.40% respectively. Analysis of ITS, act, rpb2 and tef1 genes
showed homology of strains NAIMCC-F-01938 and NAIMCC-F-01965 to T. afroharzianum
and T. asperelloides respectively. Both isolates produced an array of enzymes implicated in
bio-control activities. In co-culture studies on grape leaves, Trichoderma hyphae grew towards
and coiled around Erysiphe necator conidia, caused distortion of conidial structure and
overgrew the powdery mildew colonies. T. afroharzianum showed higher tolerance to
fungicides commonly used in powdery mildew management. In the large scale demonstration
trial it showed 43% reduction in disease severity as compared to the untreated control.
Furthermore, introducing two late season T. afroharzianum applications in a fungicide spray
schedule, as replacement for sulphur, enhanced powdery mildew control by 31% as compared
to the only fungicide schedule. The study shows that the T. afroharzianum strain NAIMCC-F-
01938 can be positioned with safe fungicides for enhanced control of powdery mildew in
vineyards.
Using Bacillus spp.
A study was conducted to identify bacterial antagonists for biological control of
anthracnose. Two hundred and ninety three bacteria were isolated from the grape ecosystem of
43 spatially distant vineyards in peninsular India. Of these, twenty-five isolates inhibited the
radial growth of C. gloeosporioides by more than fifty percent in dual culture test, and by 76-
86% and 50-74% through production of toxic non-volatile and volatile metabolites. In in vivo
studies, eighteen isolates significantly reduced anthracnose PDI. Of these eighteen isolates,
five and three isolates significantly reduced PDI of downy mildew and powdery mildew

diseases, too, respectively. These isolates, DR-38, DR-39, TL-171, DRo-198, TS-204, TS-205,
and DR-219, were identified as Bacillus spp. based on morphological and molecular
characterization, and were selected for bio-efficacy trials. Aqueous suspensions of these
isolates were applied as foliar sprays at the concentration of 1×108 cfu/ml on field grown vines.
The anthracnose PDI and the AUDPC (area under disease progress curve), was significantly
lower in vines treated with all Bacillus isolates, except DRo-198, as compared to the untreated
control. Isolates TS-204 and TL-171, recorded lower PDIs and AUDPC as compared to the
other five isolates and are identified for biocontrol of anthracnose in grapes.
For management of fungicide resistant pathogen population
The present study was undertaken to identify a promising bio-control bacteria for
management of resistance problem. Eighty-seven bacteria were isolated from mature healthy
shoots of Thompson Seedless grapevines located at ICAR-National Research Centre for
Grapes, Pune. Ten bacteria inhibited the radial growth of C. gloeosporioides isolates by 77.59%
to 84.59% in dual culture assay, 29.78% to 82.15% and 44.67 to 82.80% by production of
volatile and non-volatile metabolites, respectively. All ten bacteria produced bio-films and
controlled anthracnose in detached leaf bio-assay. Four bacteria which recorded less than 1.00
disease ratings were evaluated at three concentrations on field grown vines. Vines treated with
bacteria TS-31, TS-45 and TS-46 recorded low disease severity even at the lowest
concentration of 104 CFU/ml and area under disease progress curve (AUPDC) were 456.31,
355.31, and 446.25 respectively; while in fungicide control and untreated control AUDPC was
435.19 and 756.88. In detached berries, TS-45 showed almost complete control of berry
infection (2.38 ± 4.12%) while TS-46 (20.39±3.33) and TS-31 (35.46±32.65) were less
effective. These three promising bio-control bacteria were identified as Bacillus
amyloliquefaciens based on morphological, biochemical and molecular characterization.
Furthermore, the bacteria were compatible with commonly used fungicides indicating their safe
use in integrated disease management (IDM) also. From present study, B. amyloliquefaciens
strain TS-45 is identified as a promising bio-control agent for control of grapevine anthracnose.
Mass multiplication and formulation of ISR and bio-control strains
Trichoderma
The liquid medium for mass production of Trichoderma asperelloides 5R was
standardized as potato dextrose broth amended with 5.0% malt extract and adjusted to pH 4.72.
After incubation for 8 days under natural daylight conditions at 28 ± 4⁰C, the broth provided
1.12 × 109 spores/ml broth.
A simple and fast method for mass production of conidia of T. asperelloides 5R on solid
media was also developed. The conidia are harvested and formulated as a wettable powder
(WP) formulation for foliar application or soil application through drip. Another formulation
was developed for soil application along with organic manure. Similarly a wettable powder
(WP) formulation for foliar application of T. afroharzianum NAIMCC-F-01938 was also
developed.
Bacillus
The culture conditions for multiplication of Bacillus species were standardized as:
Nutrient broth medium amended with 1%NaCl, pH 7 to 8, temperature 30 ⁰C, orbital shaking
at 120 rpm for 72 h. A good yield of 1.0 × 109 cfu/ml could be obtained. The cells can be
harvested by centrifugation at 5000 rpm for 5 min, washed twice with sterile distilled water
and can be stored at 00C till use.

A commercial product with B. subtilis strain DR-39 was prepared by a collaborative
partner, M/s Zytex Biotech Pvt. Ltd., Mumbai. The firm developed the technology for
commercial scale multiplication of B. subtilis DR-39 and its formulation as a Wettable Powder
(WP) containing 1 x 109 cfu/g formulation with required shelf-life.
Optimizing the positioning of microbial formulations in a bio-intensive disease
management schedule for sustainable and safe grape production
Development of strategy
A strategy was prepared based on research information generated under these projects,
with higher use of microbial agents and use of fungicides only during high risk periods. The
main components of the strategy were:
Reducing primary inoculum
Spray of Trichoderma asperelloides 5R before fruit pruning in October to enable
Trichoderma to grow on senescing grape leaves and kill the inoculum of anthracnose, downy
mildew and powdery mildew present on leaves and stem. The antagonism of Trichoderma
would have continued in soil when the leaves are shed before pruning.
Increase resistance of grapevines to diseases
Soil application of liquid formulation of Trichoderma asperelloides 5R, starting at two
weeks before fruit pruning in October and continuing thereafter, to prime the vines to produce
defense related bio-chemicals and enzymes.
Biological control of diseases
Foliar applications of liquid formulations of T. afroharzianum NAIMCC-F-01938 and B.
licheniformis TL-171 at different growth stages during the fruiting season.
Restricted use of fungicides
Minimum application of single site action fungicides; restricting them during the high
risk early growth period; and use of sulphur thereafter.
Bio-degradation of pesticide residues
Foliar applications of Bacillus subtilis DR-39 to enhance bio-degradation of pesticide
residues and bring their residues to below detectable levels at harvest.
Implementation of the strategy
Demonstrations were planned at ICAR-NRCG and at three locations on growers’
vineyard and successfully implemented. The required quantity of Trichoderma and Bacillus
formulation for field applications was prepared in the laboratory.
In all bio-intensive plots (BIP) , 2-3 drenches of Trichoderma asperelloides 5R and 4-5
sprays of T. afroharzianum NAIMCC-F-01938 were given as soil drench and spray
respectively for inducing systemic resistance in grapevines and for foliar control. Only few
systemic fungicides were used at high disease risk periods, based on weather and crop growth
stage based disease predictions. The farmers practice plots (FPP) were maintained as control.
The strategy was implemented for two consecutive fruiting seasons. B. subtilis DR-39 was
applied twice in the 30 day before harvest period.
In general, the number of applications of systemic fungicides and the number of
detections were at par or lower in bio-intensive plots. The maximum number of detections was
5 and residues were below maximum limits in both plots. The cumulative residue levels were
less in bio-intensive plots. The disease severities were on par in both plots. The shelf-life was

extended by one day in bio-intensive plots. An unanticipated effect of these trials was that the
farmers got the confidence to minimize the fungicide sprays in their own practice block, too.
30. A holistic analysis for the absence of Botrytis cinerea infections in grapes
This project was initiated to conduct a systematic scientific study encompassing all biotic
and abiotic factors to confirm the presence or absence of B. cinerea infections on grapes from
India and the reasons thereof. As B. cinerea is a common pathogen on many crops grown in
India, but was not reported on grapes.
To confirm the absence of Botrytis cinerea infections in grape, commercial and research
vineyards in Maharashtra (Sangli and Nasik) and Karnataka (Shirguppi), which were between
the veraison to harvest stage, were observed for B. cinerea infection. In all the eighty vineyards,
no typical symptoms of B. cinerea infections. Thus there were nil incidences and nil severity
of B. cinerea in field.
Thirteen laterals consisting of 6-10 berries from 10 bunches on 10 different vines were
collected for incubation studies in lab from each of the 80 vineyards. Laboratory incubation
studies followed by microscopic observations, revealed that none of the samples has fungal
growth, sporangiophores and conidia resembling B. cinerea. Furthermore, twenty three grape
samples were taken at harvest stage from those vineyards which had registered for export to
EU countries and packed and stored as for export. None of the cold stored samples showed
growth of B. cinerea. The analysis of available weather data to correlate it to epidemiological
requirements for Botrytis infections also predicted no chance of B. cinerea infection and no
symptoms of the disease was observed even in the blocks where Botrytis was artificially
inoculated. Thus the required weather conditions for infection and disease development were
not present. The experiments to check for the presence of spores of B. cinerea in soil and air
also gave negative results. The experiments to check pathogenicity and virulence of Botrytis
cinerea isolates on grapes also showed absence of Botrytis cinerea during harvest.
The results of this study clearly indicate that grey mold caused due to B. cinerea is not
present in the natural environment in vineyards across Maharashtra and adjoining areas of
Karnataka. The study also showed that there is no inoculum of B. cinerea in the vineyard air
and soil. However, there are chances of infection if B. cinerea inoculum is deliberately
introduced during bloom under high rainfall periods.
31. Studies on the biology and control of the fungi causing anthracnose disease
in grapes (25.06. 2009 - 31.03.2014) and ORP on 'Diagnosis and management
of leaf spot diseases of field and horticultural crops' – grapes
Epidemiology and weather-based forecasting model for anthracnose of grape under the
semi-arid tropical region of Maharashtra
The correlation between weather parameter and PD showed that cumulative rainfall was
significantly and positively correlated, while Tmin was significantly and negatively correlated
with PDI, individually during all the three years. Tmax was also negatively correlated with the
PDI of respective years. RH1 and RH2 were positively correlated with PDI, except RH1 in
2012, where a negative correlation (non-significant) was seen. Correlation analysis with the
three years pooled data showed significant negative correlation of Tmin and Tmax with PDI,
while RH1, RH2 and CRF showed significant positive correlation with PDI. Thus, it is clear
that temperature, relative humidity, and rainfall, all play an important role in anthracnose
disease progression.

Development of a SCAR marker for the identification of carbendazim resistant isolates
of C. gloeosporioides
Carbendazim resistant and sensitive isolates of C. gloeosporioides were analysed with
12 RAPD primers. A RAPD primer detected a 320 bp marker unique to highly resistant isolates.
The unique RAPD marker was sequenced and a SCAR marker was developed and validated
for the detection of carbendazim resistant isolates. Sequence analysis of β tubulin genes of
fungicide resistant and sensitive isolates confirmed transversion mutation at codon 198. This
will be useful for deciding the appropriate measures for the effective control of the disease.
32 Studies on bacterial leaf spot (c.o. Xanthomonas campestris pv viticola) and its
integrated management in grapes
The disease bacterial leaf spot is characterized by water soaked, angular leaf spot which
later turns to irregular, dark brown to black necrotic region on the leaf surface. The causal
pathogen of bacterial leaf spot was identified as Xanthomonas campestris pv viticola. It is a
Gram negative, rod shaped bacteria with a polar flagella. It produces white, mucoid, glistening
and convex circular colonies on artificial medium. Phylogenetic analysis of 16S rDNA gene
exhibited more than 99% similarity with other Xanthomonas campestris pv. viticola. The
bacteria was found resistant to vancomycin, penicillin and oxacillin antibiotics. The bacteria
was also capable of infecting Mangifera indica and Citrus limon along with eight weed species.
Manjari Medika and Manjari Kishmish were found resistant to the pathogen. Streptocycline,
mancozeb, bronopol, B. subtilis and T. asperolloides showed significant inhibition of the
pathogen under in vitro conditions. Approximately, 23.1 kb size of indigenous plasmid was
isolated from bacteria which could be cured with three different curing agents viz., sodium
dodecyl sulphate, ethidium bromide and acridine orange. Cured bacteria was found was
sensitive to vancomycin, penicillin and oxacillin, and exhibited different colony morphology
and reduced pathogenicity. Based on morphological, biochemical and molecular
characteristics, it was identified as Xanthomonas campestris pv. viticola.
33. NRCG - DIPS - A system for diagnosis and management of important
diseases and insect pests of grapes
An online software ‘Grapevine Disease and Pest Diagnosis System for India’ has been
developed. This user friendly software assists in diagnosis by showing pictorial and textual
description of characteristic problem symptoms. The web application assists in diagnosis based
on the inputs from the user in the form of affected part and symptoms observed by him/her and
accordingly providing the relevant photographs and textual description of the related problems
to the user. The software will be useful when the management of grapevine diseases and pests
(including management based on risk assessment and forecast systems) fail and there is need
for diagnosis of these problems in the vineyard.
34. Multi-pronged strategy for management of mealybugs in grapes
Species diversity
Maconellicoccus hirsutus, Planococcus citri and Nipaecoccus viridis were found
infesting grapevines in vineyards. Phenococcus solepsis was also observed on weeds in the
vineyard but it was not found to establish on grapevines.
Development of insect and mite pest risk assessment and advisory system
Insect and mite pest risk assessment and advisory system (IMPRAS) was developed on
PHP/MySQL based interactive-web-platform to forecast pest risk levels based on forecasting
models and to provide automated pest management advisory based on calculated pest risk-

level, phenology, and actual/forecast weather conditions, previous sprays given and actual pest
level for thrips, mealybugs, flea beetle, leafhoppers, caterpillars and red spider mites. The field
calibration of this advisory system was done. The field validation of IMPRAS resulted in equal
protection against thrips, leafhoppers and caterpillars in both automated advisory and expert
manual-advice plots. However, the mealybug and mite infestations were lower in expert
manual-advice plot. The number of sprays in automated advisory plot (11 sprays) were also
higher than the expert manual-advice plot (9 sprays). IMPRAS was recalibrated for automated
advisory for mealybug and mites. It resulted in equal protection against thrips, mealybugs,
leafhoppers, flea beetle, red spider mites and caterpillars in both automated-advisory and expert
manual-advice plot with equal number of sprays (12 sprays) in both the plots. Application
programming interface (API) service was also developed for commercialization of this
technology.
Evaluation of various potential biological control agents and their compatibility
Two entomopathogenic fungi were isolated from the field infected insects and were
identified as Metarhizium brunneum (Petch) and Beauveria bassiana (Bals.). The
pathogenicity study showed that both the fungi were capable of infecting Maconellicoccus
hirsutus (Green). LC50 values 1.4 X 106 and 1.0 X 107 conidia per ml were recorded for M.
brunneum and B. bassiana, respectively. The compatibility of seven insecticides (emamectin
benzoate, tolfenpyrad, imidacloprid, clothianidin, buprofezin, fipronil, spirotetramat) with
these entomopathogens was evaluated under laboratory conditions and imidacloprid and
emamectin benzoate were most compatible and tolfenpyrad and spirotetramat were highly
incompatible with both the entomopathogens. In another study, an entomopathogenic
nematode, Heterorhabditis indica was found to cause mortality of adult females of M. hirsutus
in vitro.
Evaluation of surfactants for efficacy
Critical micelle concentration (CMC) of three surfactants, viz., sodium dodecyl sulphate
(0.3%), trisiloxane polyether (0.01%), alkyl aryl alkoxylate 80% + fatty acids 20% (0.01%)
were Sodium dodecyl sulphate at dose above CMC, trisiloxane polyether and alkyl aryl
alkoxylate 80% + fatty acids 20% at dose above CMC, at CMC and below CMC were found
to remove mealy covering from adult females of pink mealybug, Maconellicoccus hirsutus
Green significantly. Trisiloxane polyether at dose above CMC was found to cause significantly
higher mortality of third instar of M. hirsutus. Surfactants alone were found to cause significant
mortality of mealybugs. Trisiloxane polyether was found to be most effective in removing the
waxy covering of second and third nymphal instars and adult females. Trisiloxane polyether in
combination with methomyl proved to be most effective in reducing mealybug population.
Polyether trisiloxane was also found most effective in removal of mealybugs and waxy
substances from bunches.
Evaluation of new generation chemicals
Spirotetramate 150 OD @ 105 gram active ingredient per hectare was found effective
against mealybugs.
An intestinal bacterium from Heterorabditis indica was isolated and cultured on
Maconkay Agar media. Both the bacteria and EPN resulted in 100 per cent mortality of adult
M. hirsutus females in vitro and their pathogenicity was proved.

35. Management of stem borer in grapes
Species diversity
Four different species of stem borers have been observed infesting vineyards in
Maharashtra. Three of them were identified as Stromatium barbatum and Celosterna scabrator
(Cerambycidae: Coleoptera) and Dervishiya cadambae (Cossidae: Lepidoptera). The fourth
unidentified species belonged to Cossidae family in Lepidoptera order.
DNA barcoding and morphometric analysis
DNA barcoding was done for the correct identification of S. barbatum. Morphometric
analysis and pictorial illustrations of important morphological features and life-stages were
also documented for future reference. The barcode showed 86 per cent match with closely
related S. longicorne and in Neighbor Joining analysis formed monophyletic clade with S.
longicorne suggesting correct genus-level identification. The barcode was put to test for
confirmation of size-polymorphism. Cytochrome c oxidase-1 regions were sequenced for
morphologically identified S. barbatum large male (2.6 cm), large female (2.9 cm), small male
(1.6 cm) and small female (1.8 cm). These sequences showed ≤2.5 per cent divergence among
each other and ≤2.5 divergence with the DNA barcode confirming that the four specimens of
different sizes belonged to same species and variation was due to size-polymorphism.
Sex differentiation and chromosomal evolution
Karyotyping were carried out to determine chromosomal basis of sex differentiation and
evolution. Adult S. barbatum has diploid chromosome number 2n = 18+XO in male and 2n =
18+XX in female. Presence of supernumerary chromosome in female S. barbatum,
translocation and uncommon pairing behaviour were also encountered during the study.
First report of occurrence of Dervishiya cadambae on grapes
Insect damage symptoms in one vineyard was found to be due to D. cadambae
(Lepidoptera: Cossidae). Among 12 infested vineyards surveyed during 2017-18, 12-72 per
cent vines were found infested with D. cadambae in Sangli and Nashik districts of Maharashtra
state of India. The DNA barcode of D. cadambae grouped in clade of Cossidae samples. To
our knowledge, this is the first report of D. cadambae occurrence on grapes, Vitis vinifera
worldwide. Already reported hosts for this species are Ficus sp., Mangifera indica, Diospyros
melanoxylon, Tectona grandis and Nauclea cadambae. A morphologically distinguishable
larva of stem borer was found infesting grapevine at Manerajuri, Sangli. The symptoms of
infestation were similar to D. cadambae.
Natural infection of D. cadambae
Two bioagents were found causing natural infestation to D. cadambae larva. Analysis
identified one of the fungal bioagents as Metarhizium robertsii.
Laboratory rearing methods
Methods were developed for stem borers which helped in carrying out in vitro studies.
An artificial food source was identified on which C. scabrator grub was able to complete life
cycle. For S. barbatum, ground (crushed) grapevine stem was found effective for rearing grubs.
Both these methods made in vitro experiments a possibility where non-destructive real-time
observations can be taken.

Identification of emergence pattern of adults of stem borer
The period of maximum adult emergence of S. barbatum was found to be first fortnight
of June at Pune location in light traps and enclosed grapevine stem studies. Male to female
ratio of S. barbatum was found to be 1.00:1.02.
Entomopathogenic nematode (EPN)
Heterorhabditis indica@ 25 infective juveniles/ml recorded 85.65 per cent mortality of
larvae of stem borer, Stromatium barbatum. Comparative in vitro experiment against two
species simultaneously showed 100% mortality of S. barbatum grubs against 0.0% in C.
scabrator indicating that the H. indica was not effective against C. scabrator.
Isolation, identification and bioassays of EPN gut-bacteria
16s rRNA gene sequencing of bacteria isolated from EPN showed sequence similarity
with Alcaligenes Sp. and Bordetella sp. Morphologically these were gram negative, motile and
short rods. These isolates were characterised biochemically. Further, six bacteria were isolated
from haemolymph of S. barbatum larva infested with EPN and were found to cause mortality
of S. barbatum when injected into haemolymph.
Insecticides against eggs and adults of stem borer, S. barbatum
Chlorpyrifos 20EC @ 2.0 ml/L, Lambda cyhalothrin 5 CS @ 0.5 ml/L and
Cyantraniliprole 10 OD @ 0.7 ml/L were found effective in causing more than 93 per cent
mortality of eggs of S. barbatum. Fipronil 80 WG @ 0.05g/l and lambda cyhalothrin 5 SG @
0.5 ml/l resulted in maximum mortality of 100 and 96.3% when applied at the rate of 200
microlitre per adult S. barbatum during in vitro experiments out of nine insecticides evaluated.
Bio-agents (Metarhizium anisopliae and Beauveria bassiana) effective against grubs of
stem borer, Celosterna scabrator were identified and field evaluated.
Behavioral bioassays on attraction towards food sources
It was found that 48.33% females were attracted towards wet wood followed by 33.33%
towards dry wood. However, 59.17% males showed attractiveness towards dry wood. When
observations in vineyards were taken on presence of adults on vines, it was found that majority
of adults were found on cordons followed by main trunk. This information is useful in targeting
the insecticides towards main trunk and cordons for the management of adults which is the
only weak link in the life cycle of this species.
Mechanical Management
For mechanical management of stem borer, S. barbatum, wood protectant, sealer cum
healer, white cement + red soil and IIHR organic formulation were evaluated. Sealer cum
Healer found to reduce egg laying significantly.
Light traps (modified UV range) were found to attract adults of S. barbatum during rainy
season; however they were ineffective in managing the stem borer infestation in the vineyards.
The maximum infestation of stem borer was found on vines within 18 feet circle of light traps.
Among other insects, chafer beetle, jassids and moths were the major insect catches. Two light
traps viz., modified UV and easy-to-make mixed light trap were evaluated for their comparison
in attracting stem borer beetles, moths, leafhoppers and termite adults. Both the traps did not
show any statistical difference in insect catches of stem borer, moths and termites. However,
modified UV was superior over mixed light in catching leafhoppers. When compared between
stem borer male and female, it was found that significantly higher males were caught in the
light traps.

36. Consortium Research Platform on Borers in Network Mode
Mating behaviour, oviposition and hatching pattern were studied in Stromatium
barbatum. Similarly polygamy and polyandry was observed.
Thirty three morphologically different bacteria were isolated and characterised from the
gut of S. barbatum by 16S rRNA gene sequencing, FAME and enzyme analysis. Molecular
and phenotypic analysis of the isolates confirmed the affiliation of bacteria to phylum
Firmicutes (55%), Proteobacteria (25%) and Actinobacteria (20%). This preliminary
information of culturable bacterial community of insect gut would contribute to our existing
knowledge of microbes associated with S. barbatum which would in turn help in formulating
biological control of this pest.
37. Insect biodiversity in grapevine ecosystem with emphasis on economically
important grape pests
65 insect species from 12 orders and 61 families have been collected from grapes and
documented. One snail species, Giant African Snail, Lissachantina fulica (Achantinidae;
Gastropoda) was reported infesting grapes in Nashik region. The different life stages of
economically important insects and natural enemies were preserved as dry mounts and wet
specimens in 70% ethanol.19 natural enemies of insects were documented and identified under
grapevine ecosystem. Technology of growing mustard in the intra rows of vineyards to increase
the population of encyrtid parasitoid, Anagyrus dactylopii for natural parasitization of
mealybug. Stapling egg cards of Chrysoperla @ 30 eggs/card/vine over the leaves for the
management of mealybugs in grapes was found effective. Protocol for mass rearing coccinellid
predator, Stethorus rani under in vitro conditions was developed.
38. Out Reach Programme on Management of Sucking Pests in Grapes
Four bacterial isolates viz., Staphylococcus pasteuri, Bacillus firmus, B. Flexus, B.
aquimaris and control were tested for their attraction towards adults of Chrysoperla zastrowi
sillemi under cage conditions. Plants sprayed with S. pasteuri bacterial suspension (@ 2.8 x
108 cfu/ml) recorded significantly the highest oviposition (13.60 eggs/day/female) of
Chrysoperla zastrowi sillemi adults.
Identification of volatiles present in honeydew of mealybugs and establishment of their
role in the attraction of Anagyrus dactylopii. Ten bacterial isolates, viz., Micrococcus luteus,
Kocuria rosea, Bacillus aquimaris, Exiquobacterium aqauticum, Staphylococcus pasteuri,
Bacillus oceanisediminis, Bacillus flexus, B. firmus, Microbacterium testaceum, Pseudomonas
oryzihabitans were isolated from the honeydew of pink mealybug, Maconellicoccus hirsutus.,
and identified at morphological and molecular level.
QRT Comments
 Guidelines were developed for safe and sustainable grape production based on strategic
research involving the study of fungicide resistance in pathogen, induction of systemic
resistance in vines, use of naturally occurring antagonists in biological control
culminating in the production of “zero residue grapes” acknowledged by grape exporters.
The research achievements during the period under report pertaining to suppression of
insect pests in grape eco-system are relevant, encouraging and satisfactory.
 A survey may be taken up to assess the impact of bacterial leaf spot on yield. Alternate
 Biologicals are used for inducing resistance to fungal pathogens, biochemical basis for
resistance may be studied.

 Bacterial isolates from the honeydew of mealybugs for their attraction and effect on
systematic and focused approach to identify volatiles and to plan further applied research.
 The basic work on pheromone has generated interesting information. Based on the
preliminary observation of attraction of stem borer towards dead wood, research on
apneumones (semiochemicals) concept needs to be exploited. The compound with
semiochemical activity may be identified. Necessary equipment required for its analysis
may be procured.
 Resurgence of minor pests may be monitored regularly. Alert towards the occurrence of
new pests such as Fall army worm is highly essential and anticipatory research in this
regard is suggested.
 The bio-intensive strategy for insect management should be strengthened. Bio-control
agents like Cryptolaemus montrouzieri, Scymnus coccivora, Chrysoperla carnea and
entomopathogenic fungi like Metarhizium anisopliae, Beauveria bassiana and Nomurea
rileyi should be evaluated against the insects infesting grapes.
 Work on machine learning and artificial intelligence for insect detection and
management is good initiative. Utilization of ICT (Information Communication and
Technology) tools in the process of disease and insect pest management decision making
deserves appreciation.
 Research on Nano pesticides against insect pests may be initiated.
V. Development of pre- and post-harvest technologies for processing of
grapes and value addition
39. Identification and evaluation of β-glucosidase producing yeast strains and its
impact on wine quality
A total of 27 (7 of NRCG, 10 from VSI and 10 from IIHR) yeast strains collected from
different institutes were screened for β- glucosidase activities. No strain gave positive result on
reported medium however strains SPR and VSI 1106 gave positive response on modified
medium (M2) which consisted of Peptone, yeast extract, dextrose and ferric ammonium citrate
and pH 6.5. This medium was further modified by replacing ferric ammonium citrate with
ferric chloride. On this medium, both the strains gave positive results on 3rd day. β-glucosidase
activity of positive yeast stains was estimated and was found to be higher in VSI 1106 strain
than SPR. The must of Cabernet Sauvignon was inoculated with SPR, VSI 1106 and SPW
yeast strains. A total of 69 aroma compounds were recorded in these wines. The wines
produced by using positive strains for β- glucosidase were having 13 specific aroma
compounds which were absent in wines produced from the normal strain. With the increase in
pH and sugar levels, the activities of these strains were decreased. Negative strain i.e. SPW
performed better than positive strains in case of different sugar levels.
40. Winery by-products utilization for value addition in food products
Wine Lees
Fine wine lees collected from second racking of Cabernet Sauvignon wines was
processed used for ice cream making. Wine lees @ 35 g/kg recorded maximum content of
antioxidants, phenols, anthocyanins, tannins and protein as compared to other treatments. Also,
with the increasing wine lees the values of pH, specific gravity and overrun were decreased;
however increase in viscosity, acidity, colour intensity and fat destabilization index was

reported. In sensory organoleptic test, wine lees concentration of 25 g/kg ice cream was most
acceptable to the consumers in terms of flavor, texture, body and taste; however ice cream with
35 g/kg had the best colour.
Similarly, wine lees was added to milk and culture was added to make yoghurt. The
absorbance of duration affected different yoghurt parameters. The pH was decreased and
acidity was increased by increasing the duration of absorbance. Beside these parameters,
protein, carbohydrate contents were also increased. More colour was noted when absorbance
duration was increased. Sensory evaluation revealed that, the more duration of absorbance was
resulted in more acceptance of the product.
Utilization of pomace powder
Processed pomace powder from different varieties was analysed for different nutritional
and functional properties. Manjari Medika was registered with higher anthocyanins content
and functional properties as compared to other varieties.
For making cookies, different levels of pomace powder (5, 10, 15, 20, 25, 35%) were
used to replace Maida. Cookies were baked at different temperature and fixed time duration of
7 min. Replacement of 15% maida with pomace was found to be optimum. Among the baking
conditions 145 °C for 17 min were found ideal for obtaining crispy cookies.
Cookies were prepared by addition of 15% pomace powder of 13 sources. The prepared
cookies were analysed for nutritional, functional, rheological and sensory properties. Cookies
having pomace powder from Manjari Medika were registered with highest functional
properties while cookies prepared from Sauvignon Blanc were recorded with highest
organoleptic score.
The processed pomace powder collected from different sources was categorized as coarse
and fine (>210 micron size was considered as coarse). Varietal differences was recorded for
different parameters like phenolic, tannins, anthocyanins, protein and colour intensity in fine
pomace. Pomace of Manjari Medika was recorded with maximum anthocyanins (59.23 mg/g)
while minimum content was in Cabernet Franc. Protein content ranged from 15.33 to 31.13
mg/g. Cookies prepare by adding coarse pomace performed better in organoleptic test.
The breads were prepared by addition of 5, 10, 15 and 20% pomace powder of Manjari
Medika. A sensory study was performed a panel having 20 members on 9 point hedonic scale.
Breads prepared by addition of 5 per cent were found better than others.
41. Studies of pre-harvest and drying conditions to improve quality of raisins
This project was undertaken to study the effect of GA3 on raising recovery and quality
and standardise the conditions for grape drying. Yield/vine was increased from 7 to 21% in
Thompson Seedless and 13 to 38% in Manik Chaman in GA3 treated grapes. Bunch
compactness reduced from 13 to 27% in Thompson Seedless and 1 to 16% in Manik Chaman.
Berry length increased from 6 to 11% in Thompson Seedless and 2 to 15% in Manik Chaman.
11 to 16% increase in skin thickness was observed in Thompson Seedless. Whereas, 11 to 17%
increase in skin thickness was noticed in Manik Chaman. Raisin recovery increased from 18 to
30% in Thompson Seedless and 14 to 23% in Manik Chaman. Raisin browning increased from
13 to 32% in Thompson Seedless and 1 to 18% in Manik Chaman in GA3 treated grapes.
Overall acceptability of raisins indicated that in Thompson Seedless best acceptability was of
raisins produced without any GA3 treatment (control), whereas, in case of Manik Chaman best
acceptability was for raisins produced from grapes with GA3 treatment at four stages. Untreated
grapes dried 1-2 days earlier than treated grapes.

The existing grape drying shed at ICAR NRCG, Pune was modified to create controlled
drying chambers with different drying conditions. Drying kinetics of grapes in different
chambers were studied. It was observed that chamber with a temperature of 40° C, and relative
humidity  15% was found best in terms of drying as compared to other chambers. Time taken
to dry grapes in this chamber was reduced to half as compared to drying grapes under ambient
conditions in both the years.
42. Phytochemical profiling and development of value added products from
grape
Under this broad project, initially an analytical method for traces level estimation of
volatiles and phenolic compounds were optimized. The volatile and phenolic profile of two
grape varieties Shiraz and Cabernet Sauvignon grown under different geographical regions of
the country was investigated. Further, the change in phenolic profile during wine fermentation
was also established.
Metabolite profiling of 25 coloured grape cultivars was done by using high resolution
LC-MS (UHPLC-Orbitrap). Different anthocyanins were detected in studied varieties by using
anthocyanin mass spectral library which was developed in house.
Taking the advantage of highest anthocyanin content in Manjari Medika, a technology
for extraction and isolation of anthocyanin by ion-exchange raisin chromatography and its
formulation to micro-encapsulated capsules by spray drying was optimized. The technology
was filed for a patent.
QRT Comments
 Identification of yeast strains producing β-glucosidase enzyme is a remarkable achievement.
Wines produced by β-glucosidase enzyme positive yeast strains were having 13 additional
specific aroma compounds. However, stability of these aroma compounds will be studied
with specific duration. Attempts should be made to commercialize these strains. Since one
of the strains belongs to Vasantdada Sugar Institute, all the matters related to sharing of IPR
may be considered during commercialization.
 Adding coarse pomace for making cookies should be studied in detail considering the
quality parameters including few rheological parameters also. Development of these value
added products should aim as a health foods.
 Since raisin industry in the country is expanding, the institute should give more emphasis
on developing technologies for grape drying including drying process, packaging,
minimizing browning during storage etc.
 There is demand for black seeded raisins and hence emphasis should be given on identifying
the suitable varieties and drying technology for black raisins.
 Studies on chamber drying of grapes should continue and improvement in drying shed,
optimization of drying parameters and pre-treatment may be further researched on to prevent
or control the raisin browning. Economics of raisin making by chamber drying should be
established.
 The possibility of collaboration with Mahatma Gandhi Institute for Rural Industrialization,
Wardha may be explored for solar drying of raisin as they have big facility for solar drying.
 In future study, volatiles and phenolic compounds obtained from grapes should be micro-
encapsulated and used for the development of value added products may be termed as health
foods targeting specific functionality.

 Quality assessment of wines and raisins by organoleptic test should be by expert panel tested
for their ability to distinguish and grade different levels of quality parameters.
 The performance of developed de-buncher may be compared for loose and tight clusters
with reference to drying rate and microbial load.
 Effect of raw material on quality, making process and packaging and storage on browning
of raisins may be considered.
 2A clone of Thompson Seedless raised on 110R rootstock should be explored for making
good quality raisins.
VI. Food safety in grapes and its processed products
43. Investigation on dissipation of new generation pesticides with reference to
changing MRLs and establishment of Pre-harvest interval
One of the main objectives of the agricultural chemistry/NRL division of the Centre is to
establish the Pre-harvest Interval (safe waiting period (PHI)) with respect to EU-MRL through
supervised field trials for pesticide residue management. During the period, the PHI values
were established for the pesticides such as kresoxim methyl (30 days), hexithiazox (32 days),
cyantraniliprole (70 days), spirotetramate (18 days), mancozeb 75% WP (30 days),
picoxystrobin (> 90 days), amectrotradin +dimethomorph (31 days), fluxapyroxad +
pyraclostrobin (60 days), metrafenone (22 days), fluopyram + tebuconazole (60 days),
spirotetramat 120 + imidacloprid 120 SC (26 days), spirotetramat 150 OD (10 days),
imidacloprid 17.1% w/w SL (30 days), fluxapyroxad +difenoconazole (45 days), boscalid +
pyraclostrobin (55 days), dimethomorph + metiram (45 days) and valifenalate + mancozeb (60
days).
Similarly, for residue management in raisins, processing factor (concentration factor) for
pesticide residue during raisin preparation was established: 1.95 - 2.09 for famoxadone, 1.35
- 1.99 for cymoxanil, 1.10 - 1.34 for pyraclostrobin and 1.01-1.31 for metiram, 0.16 to 0.85 for
carbendazim, 0.04 to 0.09 for picoxystrobin; 0.045 to 0.17 for emamectin benzoate; 0.004 to
0.11 for methomyl. The dissipation and PF values were established for dinotefuran, flonicamid,
kresoxim methyl etc.
For risk assessment purposes and development of package of practices for production of
residue-free grapes, several grape samples for domestic market were screened in collaboration
with Maharashtra Rajya Draksha Bagayatdar Sangha (MRDBS), Pune to identify Zero residue
plots.
As one of the mandate of the residue monitoring program and extension of NRL activities
to other fruits and vegetables, dissipation study of fipronil, imidacloprid, carbendazim,
kresoxim methyl, difenoconazole, flubendiamide, buprofezin, and hexaconazole was
conducted on okra. Similarly, the dissipation of kinetics of imidacloprid, carbendazim,
kresoxim methyl, flubendiamide, lambda cyhalothrin, hexaconazole and captan and propargite
and bifenthrin in okra and tomato was studied in brinjal for estimation of PHI and half-life.
44. Development and validation of analytical protocols for multi-class multi-
residue pesticides in different agricultural commodities and processed
products
Many Multi-residue methods for analysis of pesticide residues and plant growth
regulators were developed and validated as per DG-SSANCO/DG-SANTE guide line with

respect to accuracy, precision (70-120%), LOQ (0.01 mg/Kg) and matrix effect. During the
period of review, the following analytical protocols were developed in various commodities.
 Buffered ethyl acetate method for multi-residue analysis of pesticide residues in fruits
and vegetables by LC-MS/Ms and GC-MS/MS.
 Multi- residue analysis of pesticide residues in raisin by LC-MS/MS and GC-MS by
ethyl acetate extraction.
 Improved residue analysis method for paraquat and diquat in fruits by UPLC-MS/MS.
 Improved analysis of captan, tetrahydrophthalimide, captafol, folpet, phthalimide and
iprodione in fruits and vegetables by LC-MS/MS.
 Targeted screening method for analysis of pesticide residues in difficult matrices by
high resolution LC-MS.
 Multi-residue PGR analysis by LC-MS/MS using Hilic or hypercarb column.
 GC-MS method for the estimation of dithiocarbamate fungicide residues in grapes and
other fruits and vegetables.
 Multi-residue analysis of >200 pesticides in different spices (cardamom, chilli powder,
cumin, coriander, pepper, turmeric) by LC-MS/MS and GC-MS/MS analysis in
collaboration with Spices Board, Cochin.
 Unified method for extraction and analysis of veterinary drugs and pesticide residues
in milk by LC-MS/MS.
 Multi-residue method for analysis of pesticide residues in products of animal origin.
 Residue analysis method for streptomycin and tetracycline in pomegranate.
 Multiresidue analysis of multiclass veterinary drugs in foods of animal origin.
 Accelerated solvent extraction techniques for pesticide residues in Yam and taro.
 Multiresidue analysis of multiclass pesticides and polyaromatic hydrocarbons in fatty
fish by GC-MS/MS in collaboration with CIFT, Cochin.
 Simultaneous analysis of the residues of four herbicides viz. pendimethalin,
oxyfluorfen, imazethapyr and quizalofop-p-ethyl in peanut in collaboration with DGR,
Junagadh.
 Multi-residue method for pesticide residue analysis in edible oils.
45. Comprehensive Screening of Target, Non-target and unknown Emerging
Organic Contaminants in Fruits and Vegetables by GC-MS and LC-MS
Development of a screening method based on accurate mass analysis
An LC-QToF MS (High Resolution Mass Spectrometer) was utilized for non-target
residue monitoring study. Since diverse contaminants might appear in any food commodities,
a large spectral library was developed for food safety screening of extracts containing
pesticides, plant growth regulators, and other food contaminants. The samples were analysed
for >200 chemicals in full scan as well as MS/MS scan mode. Considering the fact that the
food contaminants are of small molecular mass, in the full scan MS mode, the mass range was
set between 100-1000 amu. In the MS/MS mode, the mass range was 50-1000 amu. The
identification of analytes was carried out by means of accurate mass measurement with mass
accuracy ≤ 5 ppm of error.

Development of a generic sample preparation method for extraction and analysis of about
300 contaminants using LC-MS/MS
A generic method of sample preparation using methanol as extraction solvent has been
validated for fruits and vegetables for analysis of pesticides, plant growth regulators and
mycotoxins. The LC-MS/MS parameters were optimized for an integrated instrumental method
for the residue analysis of pesticides, plant growth regulators and mycotoxins. The limit of
quantification for all the analytes were below 0.01 mg/L fulfilling the MRL requirements. The
analysis for the 300 compounds could be completed within 20 min.
Development and validation of method for determination of 277 pesticides in wines using
GC-MS/MS
A selective and sensitive method was developed and validated for determination of 277
pesticide residues in relatively complex matrix i.e. wine (processed product of grape). The
method involved extraction of 10 mL wine with 3 mL methyl tertiary butyl ether (MTBE) in
presence of 350 µL of ammonia.
Validation of a GC-MS method for estimation of dithiocarbamate fungicide residues and
safety evaluation of mancozeb in fruits and vegetables
A sensitive and rugged residue analysis method was validated for the estimation of
dithiocarbamate fungicides in a variety of fruit and vegetable matrices. The sample preparation
method involved reaction of dithiocarbamates with Tin (II) chloride in aqueous HCl. The CS2
produced was absorbed into an isooctane layer and estimated by GC–MS selected ion
monitoring. Limit of quantification (LOQ) was ≤ 40 µg/kg for grape, green chilli, tomato,
potato, brinjal, pineapple and chayote and the recoveries were within 75-104% (RSD < 15% at
LOQ).
Development of a buffered ethyl acetate sample preparation method for extraction and
analysis of 296 contaminants using LC-MS/MS
A buffered ethyl acetate extraction method is developed for the simultaneous analysis of
296 agrochemicals (insecticides, fungicides, herbicides and plant growth regulators) in a wide
range of fruit and vegetable matrices by liquid chromatography−tandem mass spectrometry
(LC-MS/MS). The optimized quantity of acetate buffer (1% acetic acid + 0.5 g of sodium
acetate per 10 g of sample) adjusted the pH of each test matrix to 5-6, which in turn significantly
improved recoveries of acidic and basic compounds. The role of diethylene glycol (used in the
evaporation step) on signal suppression of certain compounds was evaluated, and its quantity
was optimized to minimize such an effect. The method was validated in grape, mango,
drumstick, bitter gourd, capsicum, curry leaf, and okra as per the DGSANCO/12571/2013
guidelines. Recoveries in the fortification range of 1−40 μg/kg were within 70−120% with
associated relative standard deviations below 20% for most of the compounds. The method has
potential for regulatory and commercial applications with a generic approach.
Dissipation studies of pesticides in fruits and vegetables
Field experiments for the study of persistence and dissipation behavior of picoxystrobin,
cyantraniliprole, ametoctradin and dimethomorph, fosetyl-Al and fluopicolide were carried out
to estimate their pre-harvest intervals for recommendation to grape growers. Similarly the
dissipation behavior of propargite and bifenthrin were also studied in tomato and okra for
estimation of PHI. The PHI of Bifenthrin in okra was 1 day for single dose. For propargite, at
single dose, the initial residue found was below MRL whereas for double dose of application
the PHI’s of bifenthrin and propargite were 1.5 and 1 day, respectively. The PHIs of bifenthrin

and propargite in tomato for single dose were not calculated as the initial residues were below
MRL whereas for double dose of application the PHI’s of both chemicalswas 1 day.
46. Nutritional quality and safety evaluation of common processed products of
grape (FSSAI funded)
Nutritional profiling of raisin samples collected from Nasik, Pune, Solapur and Sangli
and Vijayapur district of Karnataka was carried out for amino acid, total sugars, minerals, total
starch, total phenols, total proteins and carbohydrates. Total carbohydrates (g/100g FW) varied
from 70.07 g to 79.9 g. Black raisins contained more carbohydrates than green raisins. Total
protein (g/100g FW) varied from 3.38 g to 4.22 g. Black raisins contained more proteins than
green raisins. Total starch (g/100g FW) varied from 1.05 g to 1.37 g. Black raisins contained
more starch than green raisins. Total phenols (g/100g FW) varied from 0.14 g to 0.27 g. Black
raisins contained more phenols than green raisins. Calcium (mg/100g FW) ranged between
46.65 mg and 72.76 mg. Black raisins from Nasik, Pune and green raisins from Sangli
contained more Ca. Magnesium (mg/100g FW) content ranged from 9.08 mg to 38.85 mg.
Raisins from Nasik and Sangli contained more Mg. Phosphorus (mg/100g FW) content range
was 82.45 mg to 107.71 mg. Green raisins from Nasik and Pune contained more P. Sodium
(mg/100g FW) content was 11.49 mg to 15.61 mg. Black raisins from Pune, Green raisins from
Sangli, Solapur and Vijayapura contained more Na. Potassium (mg/100g FW) content ranged
from 771.42 mg to 1101.34 mg. Black raisins from Pune, Nasik and green raisins from
Vijayapura contained more Mg. Iron (mg/100g FW) content varied from 3.28 mg to 5.71 mg.
Raisins from Nasik and Pune contained more Fe. Copper (mg/100g FW) content ranged from
0.18 mg to 0.37 mg. Raisins from Nasik and green raisins from Sangli and Vijayapura
contained more Cu. Zinc (mg/100g FW) content varied from 0.20 mg to 0.32 mg. Green raisins
from Nasik and Vijayapura contained more Zn. Manganese (mg/100g FW) content was
between 0.30 mg and 0.47 mg. Black raisins from Nasik, Pune and green raisins from
Vijayapura and Solapur contained more Mn.
Total 57 samples collected from different locations were analysed for residue of
different pesticide by GC-MS/MS and LC-MS/MS. The major residues detected were lambda
cyhalothrin, cyfluthrin, chlorothalonil, cypermethrin and permethrin. In the samples analysed,
all pesticides detected were below EU MRL levels. Two samples from Nasik region were found
to be in border line for 4-Bromo-2-chlorophenol (0.01-0.011) where EU MRL is 0.01. In
Mycotoxin analysis, most of the samples were detected with aflatoxin and ochratoxin in all the
5 districts. The total Aflatoxin concentration ranged between 0.41 µg/kg to 6.73 µg/kg and
Ochratoxin ranged between 0.2 µg/kg to 2.39 µg/kg. Samples analyzed for aflatoxin were
below EU MRL level except for samples from Nasik location where total aflatoxin detected
was 6.73 µg/kg. EU MRL for aflatoxin is 4.0 µg/kg.
QRT Comments
 The major residues detected in raisins were lambda cyhalothrin, cyfluthrin,
chlorothalonil, cypermethrin and permethrin. This type of information is very useful for
research as well as industry. Generation of such information should continue in future
also. Data generated should be published in form of bulletins or research articles.
 Simple to use Radio-immuno based assay method for Aflatoxins may be developed.
3.1.6 Publications
During the last five years the scientists of the Centre published 131 research articles. Of
these, 67 were in international and 64 in national journals. More than 50% of the articles were

in journals with a NAAS rating of >6.0. The year wise details and the list of publications is
given in Annexure 3.
3.1.7 Intellectual property rights
During the period of review, the Centre has filed two patents, obtained five copyrights for the
original creative work by the scientists, obtained trademark for logo under five trade mark
classes and commercialized its technologies to four agencies. The details of these patents,
copyright, trademark and technology commercialisation is given in annexure 4.
3.1.8 Honours and awards
The Centre has ISO 9001:2015 accreditation. The NRL laboratory has been accredited
by NABL for chemical testing for pesticide residues as well as for proficiency testing provider
for pesticide residues. Besides these institutional recognition, the Scientists of the Centre have
carved a niche for themselves in their respective field of specialisation as reflected by
conferring of awards and fellowship by different agencies, nomination to committees of
national importance, recognition by different universities either as Ph.D. guide or thesis
reviewer and examiner, recognition by several national and international journals either as a
member of editorial committee or reviewer. Scientists have also received prizes for their
presentation in different seminars and symposium. The details of these awards is given in
annexure 5.
3.1.9 Inter-institutional collaboration

During last five years, the Centre has collaborated with 17 institutes/agencies for research
as well as technology transfer. Collaboration was for research on resistance breeding,
biotechnology research, development of decision support systems, development of analytical
protocols etc. The details of inter-institutional collaborations is given in annexure 6.
3.2 Review - Transfer of technology

The Centre has made concerted effort to take its technologies to the farmers and other
stakeholders. The scientists have regular interactions with the farmers and are actively involved
in extension activities.
3.2.1 Field demonstration of institute technologies

A total of 21 field demonstrations were held during the period of review. Demonstrations
were varietal demonstrations, techniques for improving water use efficiency, schedule for zero
pesticide residue and bioremediation. The details of these demonstration are given in
annexure 7.
3.2.2 Web advisory

The Centre issued weekly advisories to give weather based disease and pest risk
assessment for different grape growing regions and recommended spray schedule based on risk
assessment. Advisories also included recommended horticultural practices for hailstorm and
heavy rainfall affected vineyards, pesticide residue alerts for export grapes etc. An easily
accessible link is provided on the website of the institute to access these advisories. A total of
232 web advisories were issued during five years. The number of advisories was 41, 51, 54, 50
and 36 respectively for the years 2013-14, 2014-15, 2015-16, 2016-17 and 2017-18.

3.2.3 Participation in growers’ seminar
The scientists of the Centre regularly participated in the growers’ seminars organized by
different grape growers associations, KVKs, State Department of Horticulture, private
organizations, etc. During the period of review, scientists participated in a total of 85 growers’
seminars. The number of such seminars was 20, 11, 17, 21 and 16 respectively for the year
2013-14, 2014-15, 2015-16, 2016-17 and 2017-18.
3.2.4 Radio and TV programmes
Scientists participated in 24 radio talks and 15 TV shows during the period of report.
3.2.5. Advisory services
QRT was apprised that the scientists of the Centre give regular advisory services to the
farmers through different WhatsApp groups. The specific issues reported by the farmers are
addressed through the field visit also. Suitable advice is also given in the event of loss due to
extreme weather conditions like hailstorm and chilling temperatures.
QRT Comments
 Decision Support System; IPM informatics (by the use of ICT), Village Knowledge
Centres (VKCs) and growers’ clubs may be established on pilot basis to enable virtual
extension and research communication.
 Extension activities of the Centre must be extended to Bijapur and Bagalkot districts of
Karnataka also.
 The Centre should involve the ATARI / KVKs located in the grape growing districts of
Maharashtra and Northern Karnataka.
3.3 Review of Management issues
3.3.1 Infrastructure
Laboratories
The Centre has laboratories for Agrotechniques, Biotechnology, plant physiology, Plant
pathology, Soil Science, post-harvest technology, genetics, National Referral Laboratory.
These laboratories are well equipped and all the equipments are in working condition.
During the period of review, the Centre procured vacuum concentrator, fermenter, water
purification system, sample mill grinder, soil moisture monitoring system, tractor with spraying
unit, soil penetrometer, PCR work station, fume hood, laminar air flow, culture rack and trolley,
centrifuge, digital CCD camera for stereo binocular microscope, ArcGIS Software, software
for Leica CCD Camera, deep freezers, refrigerated shelf, Electronic balances, Air oven, pH
meters, liquid nitrogen tanks, deep freezer cum refrigerators, accessories for lyophilizer and
fermenter, insect suction sampler, destemmer and crusher, pneumatic press, orbital
environmental shaker, deep freezer, lux meter, autoclave, micrometer, hot air oven, Ice flaking
machines, electrophoresis unit, water bath, biosafety cabinet, walk-in cold chambers,
homogenizer, olfactometer, gel documentation system, -80°C deep freezers, high speed
computers, lyophilizers, spray dryer, high speed refrigerated centrifuge and microfuge during
the last five years.

QRT Comments
QRT recommends the procurement of equipment for Pheromone research, radio isotope
laboratory.
Library
The Centre’s library provides access to journal and books to all the staff. Access to most
of the journals is through CeRA. Besides, journals related to grape research which are not part
of CeRA are subscribed. Library has a few computer terminals which allow JRF, SRF and other
project staff, access to the online resources. During last five years 30 subject specific English
books and 48 Hindi books were added to the library. Nineteen English and three Hindi journals
were subscribed. Three online English journals were also subscribed besides other journals
accessible through CeRA.
Land
The Centre is spread over an area of 116 acres. The experimental vineyards are raised
over an area of 61 acres. Nursery activities are spread over 4 acres. About 2.6 acres is occupied
by residential and non-residential buildings, whereas about 43 acres of area is under services
like roads, service block etc.
QRT Comments
Additional land is required for expansion of nursery activities, establishment of hybrid
evaluation block and new laboratories. It is recommended to give back 4.0 ha of land to ICAR-
NRC Grapes, which was earlier given to Directorate of Onion and Garlic by the Centre.
Irrigation water
Water for irrigation is drawn from bore wells and from nearby canal through lift
irrigation. Two bore wells and four open wells are available for water. To tap the water through
lift irrigation of canal water, a storage tank of 50 lakh litre and another of 2 crore litre capacity
are built. In addition water tanks of 20-70 thousand litre capacity are available to cater to the
needs of office, laboratory and residential buildings. Roof top rain water harvesting system is
also in place.
Power supply
The Centre is located in the outskirts of the Pune city and draws its electricity through
rural line of Maharashtra State electricity Board (MSEB). The 24 hour generator backup
provide uninterrupted power supply to the buildings and equipment. The costly and
sophisticated equipment are operated through UPS to avoid damage due to sudden electric
surge.
Solar energy- nonconventional energy
Besides, electricity, institute meets some of its electricity requirements through a 70kW
roof top solar panel system which was installed during the period of report.

3.3.2 Manpower
Category Sanctioned Filled Vacant
Research & Management Personnel 1 1 0
A. Scientific
Pr. Scientist 1 1 0
Sr. Scientist 5 5 0
Scientist 11 10 1
Total (A) 17 16 1
B. Technical 8 8 0
C. Administrative 13 7 6
D. Supportive 7 7 0
Total 46 39 7
QRT Comments
In view of the proposed intensification of research, and develop second line of scientists
QRT recommends the creation of two posts of junior level scientist each in the disciplines of
Plant Breeding and Horticulture; one each in Biotechnology, Plant physiology, Entomology,
Food Technology.
The Centre has the sanctioned strength of only eight posts of technical staff which
includes one driver and one computer operator. Inadequate technical support is available for
research. Hence QRT recommends that more technical posts, according to scientist to technical
staff ratio of 1:1.5 may essentially be created for the Centre.
Six posts of administrative staff are lying vacant leading to extreme work load on the
existing staff. Contractual employment was found to be ineffective and uncertain. Approval for
filling the vacant posts may be accorded by the council at the earliest.
Human Resource Development
It is heartening to observe that almost all the scientific, technical and administrative
staff have undergone one or more training programme during last five years in different
national laboratories (details in annexure 8). Director and one scientist were deputed to
international organisations for study visit, participation in meetings and seminar (details in
annexure 9). Scientists were also encouraged to participate in national and international
seminars and symposia (Annexure 10), where they presented their work as oral or poster
presentation. However, it was noted that the scientists need more exposure to the international
laboratories working on grape and modern technologies.
Among technical staff, all the technicians were deputed for at least one training
program. Since all the current technical staff is engaged in farm duties, they should be
encouraged to attend more training programmes on modern farm management techniques.

3.3.3 Finance
Budget
The year-wise details of the funds received by the Centre and expenditure under different heads
during the period of review is given in annexure 11. The summary of the budget is as below
Financial Grants received Grants received Expenditure Expenditure
year under PLAN under NON- incurred under incurred under
(Rs. in lakhs) PLAN PLAN NON-PLAN
(Rs. in lakhs) (Rs. in lakhs) (Rs. in lakhs)
2013-14 380.00 465.60 369.12 390.24
2014-15 271.00 542.00 271.00 485.52
2015-16 256.06 637.00 256.04 592.08
2016-17 242.10 751.00 242.10 715.59
2017-18* 944.10 913.52
* The concept of PLAN and NON-PLAN was discontinued from FY 2017-18 onwards. From FY
2017-18, Unified Budget is being followed by ICAR. PLAN and NON-PLAN budgets have been
merged with each other.
QRT Comments
Except for establishment charges, the entire funds were utilised. However, during
interaction with the scientists and staff, it was observed that the funds under research
contingency are not adequate and many times some of the research work could not be taken up
due to non-availability of required resources. Some of the approved infrastructure work
specially construction of PHT lab could not be taken up due to shortage of funds. In some years
there was cut in budget at late stage, resulting in delayed execution of the activities. QRT also
recommends provision of funds for repair and maintenance of laboratory and residential
buildings and farm roads.
Revenue generation
The Centre generated an amount of ₹ 326.41 lakhs as revenue during last five years. The
year wise details are given in annexure 12. The important activities of revenue generation were
through sale of farm produce, sale of planting material, consultancy services, contractual
research and training programmes. During the review period, a total of 252161 rooted cutting
of rootstocks and scion varieties were distributed to growers, commercial nurseries, AICRP
Centres and other research institutes. A total of 42 consultancy programmes (annexure 13), 197
contract research trials (annexure 14) and 65 training programs (annexure 15) were conducted
during this period and contributed significantly towards revenue generation.
There is an upward trend in revenue generated. Currently it constitutes about 9-10% of
total budget and 20% of establishment charges. Considering the relatively low number of
scientific and other staff, it is a good achievement.
3.3.4 Administration
i. Institute Management Committee
The meeting of IMC is held only once in a year as against the recommended frequency
of one in three months. The IMC during its meetings, approved the execution of infrastructure
and developmental work.

ii. DPC
The QRT observed that the departmental promotion committee is held promptly for the
promotion of the staff members. No complaints regarding delay in promotion was received
from the staff members during interaction.
iii. IJSC
The Centre has Institute Joint Staff Council. However, it was noted that regular meetings
of IJSC is not held regularly.
During interaction with IJSC, there was demand for recreational facility for the staff.
Some staff reported delay in reimbursement of medical expenses.
3.4 Comparison of research progress with the previous QRT
period
A. Research output
S. Area/Discipline 2008-13 2013-18
No.
1 Widening genetic base Germplasm consists of 382 Centre has a total collection of 470
cultivars, 24 rootstocks accessions. During the period of
and nine related wild report, 7 accessions from USDA, 16
species, totalling to 415. from Leh-Ladhakh, 19 France wine
varieties, 3 from IARI, 1 from Tamil
Nadu and 11 clonal selections, 2
accessions from exporters were
added to the germplasm.
2 Evaluation and 1. Morphological 1. Germplasm was evaluated for
Characterization of characterization of 315 loose bunches, naturally bold
germplasm accessions according to berries and multiple flower buds
UPOV descriptors. and against, downy mildew,
2. Genotypes with powdery mildew, anthracnose,
naturally loose bunches mealy bugs, mites, thrips and
and bold berries were jassids.
identified. 2. 100 accessions were
3. DNA finger printing of characterized based on 35 traits.
317 genotypes was 3. 149 grape genotypes were
done. analyzed with 8 microsatellite
primers.
3 Genetic Improvement 1. Of the 61 hybrids 1. Survey for clonal selection and 9
evaluated, six were mutants were identified.
suitable for table 2. Downy mildew resistance
purpose, two for wine, breeding - 280 F1 seedlings for
one for juice and two cross combinations were
Monukka and one for field established. Screened for
Juice. downy mildew and identified 33
2. A white mutant of resistant progenies. 101 F1
Kishmish Rozavis, and seedlings evaluated for
hybrids A18-3 and horticultural traits.

No.
Medika are under field 3. Coloured table grapes: New
evaluation. breeding program initiated and
3. Manjari Naveen, a 300 inflorescences from 5 cross
clonal selection from combinations were crossed.
Centennial Seedless has 4. Mutation Breeding: 300 vines of
been released. gamma irradiated Thompson
Seedless and 50 of Red Globe are
being evaluated in field.
5. Two varieties, Manjari Medika
and Manjari Kishmish were
release at institute level.
4 BiotechnologyResearch 1. Interspecific hybrids 1. Marker assisted breeding for
obtained by embryo downy mildew: a new marker for
rescue were evaluated. RPV3 locus was identified and
2. Identification of salinity validated.
responsive transcription 2. Salt stress responsive genes were
factors using publicly identified based on in silico and
available EST database. transcriptome analysis. Probable
3. Proteomics analysis in genomic regions for salt tolerance
Thompson Seedless were identified. Polymorphic
grape. microsatellite markers identified
in 10 selected genes.
3. GA3 responsive genes were
identified at three stages of bunch
development in Thompson
seedless. Potential QTL regions
for bunch traits were identified.
Polymorphic microsatellite
markers were identified in 10
genes.
4. Stage x rootstock interaction on
proteome profile of berries of
Thompson Seedless was studied.
5 Evaluation of 1. Rootstock 110R was 1. Evaluation of rootstocks for
rootstocks superior to Dogridge for Cabernet Sauvignon in Pune
Thompson Seedless. region: 110R and 101.14MGT
2. Abiotic stress tolerance were found the most suitable.
was more in 110R and 2. Evaluation of rootstock for Red
Salt Creek. Globe.
3. Evaluation of rootstock for
Fantasy Seedless.
4. Evaluation of rootstock for
Sauvignon Blanc.
5. Ten red wine varieties and nine
white wine varieties grafted on
110-R rootstock were evaluated.

No.
Syrah and Caladoc among red
and Colombard and Riesling
among white varieties performed
better than other varieties.
6 Propagation 1. Mass multiplication by 1. Development of tissue culture
pre- treated single techniques for production of
noded cuttings of quality planting material in grape.
Dogridge.
7 Water management 1. Irrigation Scheduling for 1. Standardizing irrigation schedule
Cabernet Sauvignon on in Fantasy Seedless: The variety
110R- Initiated . require 20% less water as
2. Decision Support compared to Thompson Seedless.
System for Enhancing Subsurface irrigation led to
Productivity of Grapes further saving of water.
under Moisture and 2. Standardizing Irrigation Schedule
Temperature Stress for Cabernet Sauvignon: Growth
Conditions- Started. stage wise irrigation schedule
was developed.
3. Demonstration of techniques to
improve water use efficiency in
growers’ field: Demonstration at
three drought prone locations
resulted in considerable saving of
irrigation water without affecting
the yield.
4. Decision Support System (DSS)
for Enhancing Productivity of
Grapes under Moisture and
Temperature Stress Conditions: a
web and mobile based DSS was
developed, validated and
launched.
8 Nutrient Management 1. Techniques to improve 1. Direct and residual effects of
NUE utilizing farm applied potassium in Cabernet
waste- Started. Sauvignon vines grafted on 110R
2. Developing petiole rootstock: no response to applied
nutrient guides for potassium was observed if the
Cabernet Sauvignon on soil K levels were above 375
110R rootstock- ppm.
Initiated. 2. Diagnosis of leaf reddening
symptom in Sharad Seedless
grapevine grown under saline
irrigation: Significantly lower N
and P and high chloride and
sodium content in affected
leaves.

No.
3. Comparison different sources of
organic matter: Press mud
compost alone or in combination
was effective cheaper source of
organic matter.
4. Developing petiole nutrient
guides for Cabernet Sauvignon
on 110R rootstock: Critical levels
of N, P and K were determined at
both the stages.
5. Remote sensing for precision
farming: remote sensing data
used for developing management
zones in vineyards.
9 Canopy Management 1. Canopy regulation and 1. Standardization of planting
cluster thinning for density for producing quality
improving quality wine: a planting density of 1361
improvement in wine vines/acre (8x4 feet spacing) was
grapes. found optimum for Cabernet
2. Source-Sink in relation Sauvignon.
to different canopy 2. Training system to increase wine
architecture. quality: Mini Y trellis was better
for growth and yield parameters.
3. Standardization of pruning time
for producing quality wine:
Pruning on 1st September was
optimum for obtaining good
quality Syrah wine.
4. Plastic cover on grapevine
growth and productivity:
Grapevines raised under plastic
cover recording early sprouting,
higher yield, early harvesting and
reduced incidence of pests and
diseases.
5. Climate based spatial
delimitation of suitable grape
growing regions in India using
GIS: GIS based model developed
to delineate regions with climatic
suitability and a thematic maps
were developed.
10 Use of plant bio- 1. Testing of new 1. Standardization of bioregulators
regulators chemicals- Indigenous schedules for improving quality
Commercial products and yield of table grapes:
schedules for Crimson Seedless,

No.
for yield and quality Fantasy Seedless, Manjari
improvement. Naveen and Red Globe.
2. Bio-efficacy, phytotoxicity and
residue dissipation of
Chlormequat Chloride (CCC) in
grapes
3. Bio-efficacy of new molecules:
bioefficacy of eight commercial
formulations was tested.
11 Physiological disorders 1. Studies on pink berry 1. Physiological disorders and their
disorder in white grapes. management in grapes
2. Knot formation on - Sunburn
bunch peduncle- - Berry Cracking
initiated. - Swelling of knot on bunch
peduncle
12 Management of 1. Weather- based disease 1. Monitoring of fungicide
Diseases forecasting in grapes. resistance and developing
2. Virus diseases of grapes. mitigating strategies: molecular
3. Leaf spot diseases of basis of fungicide resistance in
grapes (ORP). major fungal pathogens was
studied and naturally occurring
4. Bio-efficacy of
antagonists were identified.
fungicides.
for 2. Development of microbial
5. Bio-prospecting
formulations for biological
viticulturally important
control of grape diseases:
microorganism (ORP).
Formulations for effective
Bacillus and Trichoderma strains
were developed and their
effectiveness was tested in field.
3. Developing bio-intensive disease
and pest management module:
The module was developed and
successfully demonstrated at four
locations.
4. Holistic analysis for the absence
of B. cinerea in grapes: Absence
of B. cinerea in Indian vineyards
was confirmed.
5. Management of Leaf spot disease
of grapes: epidemiology and
weather based forecasting model
developed. SCAR marker for
identification of carbendazim
resistant isolates of pathogen
developed.

No.
6. Studies on bacterial leaf spot:
Causal organism of bacterial leaf
spot identified and alternate hosts
were identified.
7. Online system for diagnosis and
management of important disease
and insect pests was developed.
13 Management of insect 1. Insect biodiversity in 1. Multi-pronged strategy for the
pests grapevine ecosystem management of mealybugs in
with emphasis on grapes: Species diversity
economically important analysed, insect and mite pest
grape pests. risk assessment and advisory
2. Studies on the system developed, various
management of thrips biological control agents,
and mites. surfactants and new generation
3. Multi-pronged strategy chemicals evaluated and effective
for the management of agents and chemicals identified.
mealybugs in grapes. 2. Management of stem borer: new
species identified, life cycle of
new species studied, potential of
entomopathogenic nematode
evaluated, a semiochemical was
identified.
3. Insect biodiversity in grapevine
ecosystem with emphasis on
economically important grape
pests: large number of insect
species collected, identified and
preserved.
4. ORP on management of sucking
pests in grapes: volatiles present
in honeydew of mealybug was
identified.
14 Post-harvest 1. Standardization of pre- 1. Identification and evaluation of
technology harvest factors for β-glucosidase producing yeast
production of quality strains: 2 strains were identified
red wines. and characterised. Aroma
2. Standardization of compounds specific to positive
techniques for strains were identified.
minimization of 2. Winery by-products utilization
browning in raisins. for value addition in food
products: technologies for
enriched cookies, sugar free ice-
creams, yoghurt was developed.
3. Studies on pre-harvest and drying
conditions to improve quality of

No.
raisins: Application of GA3 was
found to increase yield and
raising recovery in Thompson
Seedless and Manik Chaman.
Drying chamber for drying
grapes under Pune conditions
was designed and tested.
4. Phytochemical profiling and
development of value added
products from grape: The volatile
and phenolic profile of two grape
varieties Shiraz and Cabernet
Sauvignon studied. Change in
phenol profile during
fermentation studied. Technology
for the extraction and isolation of
anthocyanin, and its formulation
was developed.
15 Food Safety 1. Persistence of pesticides 1. Dissipation of new generation
in soil and water. pesticides with reference to
2. Persistence and changing MRLs and
dissipation of pesticides establishment of Pre-harvest
in grapes. interval. PHI for 17 chemicals,
3. Monitoring of pesticide processing factor for 8 chemicals,
residues in grapes using dissipation studies of several
new analytical methods. chemicals in okra and brinjal.
2. Development and validation of
analytical protocols for multi-
class multi-residue pesticides in
different agricultural
commodities : 16 different
analytical method were
developed and validated.
3. Comprehensive Screening of
Target, Non-target and unknown
Emerging Organic Contaminants:
methods for screening based on
mass analysis, preparation
method for analysis of 300
contaminants on LC-MS/MS,
277 pesticides in wines using
GC-MS/MS, method for
dithiocarbamate fungicides etc.
4. Nutritional quality and safety
evaluation of common processed
products of grape: raisins from 4

No.
districts were evaluated for
nutritional quality.
QRT Comments
1. More emphasis was given to biotechnological (MAS for downy mildew resistance) support
to conventional breeding during the quinquennium under review.
2. Optimization of nutrient and water requirement of grapevine in different soils. The
nutrient and water requirement of grapevine in heavy soil is optimized, as most of the grape
growing regions have this type of soil. However, the nutrient requirement depends on the
fertility levels of the soil. QRT recommends continuation of this recommendation and the
fertility levels of soil in different regions should be estimated and nutrient requirement be
optimized for different soils.
3. Basic and strategic research to be undertaken for downy mildew resistance in tropical
grapevines. The inheritance of downy mildew resistance/susceptibility has been studies in
two crossing population. The molecular marker has been developed for RPV3 locus of
downy mildew resistance in grape and being used for selection of progenies. However,
efforts should be further intensified to develop markers for downy mildew resistance, bold
berries and loose bunches, and should be validated and utilised for gene pyramiding through
breeding. QRT recommends continuation of this recommendation and intensification of
conventional breeding supported by embryo rescue and marker assisted selection.
4. Research on abiotic tolerance of 110R rootstock was not carried forward during the current
quinquennium. Multi-location trials to establish the superiority of this rootstock to Dogridge
and to trials to standardize the package of practices for Thompson Seedless on 110R were
needed.
5. Continuity in trials in crop production is missing between the two quinquennia. Over
emphasis was given to wine grape production technologies.
6. Nutrient and water requirement for wine grapes in vertisols have been standardized.
7. A breakthrough in wine research was achieved in identifying a yeast strain that produces β-
glycosidase enzyme, which increased to aroma compounds in wines.
16 Publications in peer 109 131
reviewed journals
QRT Comments
1. There has been 18 per cent increase in the number of research publications from the
Centre. However only about 50% were published in Journals with NAAS rating more
than 6.0.
B. Transfer of Technology
1. Field demonstration at Weather forecast based 1. Demonstration of Manjari
farmer’s field advisory among farmers of Medika at three locations.
grape growing regions 2. Demonstration of four wine
hybrids at Nasik.
3. Demonstration of techniques for
improving water use efficiency at
three locations.
4. Demonstration of Pest
management module for ‘zero’

No.
pesticide residues in table grapes
at three locations.
5. Demonstration of biological
control agents for disease
management at three locations.
6. Demonstration of Bacillus
subtilis strain (Strain DR-39) for
bioremediation of pesticides at
three locations.
2. Web advisories - A total of 232 weekly web advisory
were issued.
3. Participation in Scientists participated in a Scientists participated in a total of 85
growers’ seminar total of 125 growers’ growers’ seminars.
seminars.
QRT Comments
1. The varieties and Technologies developed at the Centre have been demonstrated in
growers’ vineyards as suggested by the previous QRT.
2. The plant protection schedules evolved by the Centre for producing zero residue grapes
have been successfully demonstrated in grower’s vineyards and well appreciated by the
table grape exporters.
C. Infrastructure Development
1. Laboratory and farm Works: Establishment of Works: biotechnology lab
infrastructure DUS Testing Centre for completed, NRL building, repair of
grapes was initiated on 2 glass and polyhouse, 6 acre new
acre plot, Labour shed has vineyards, tractor with ESS, farm
been constructed on the machinery, solar panel, raisin drying
farm, Compound wall is chambers and several minor works.
strengthened, Equipment: several sophisticated
Development of equipments were purchased.
demonstration block (E1- Majority of the labs are well
4.5 acre), Plant breeding equipped.
block (E5-2.0 acre) and F1
block (1.0 acre), Block A1
(2.5 acre) and part of D4
(1.5 acre) were
rejuvenated, Facility for
composting the farm
wastes, tractor drawn
Blower type Dragon
Spraying System, Raisin
shed, Lift irrigation, Toilet
facility for farm labourers,
Polyhouse / net house for
the nursery, F6 Block of 2
acres

No.
Several sophisticated
equipments were
purchased.
QRT Comments
Research infrastructure was well developed.
D. Human Resource Development
1. Training and visit Deputation abroad – 24 Deputation abroad – 10 programs
abroad programs Scientist – 25 programs
Scientists – 18 Technical: 17
programmes Administration: 8
Technical: 2
Administrative: 1
QRT Comments
General activities of capacity building except scientists’ visits abroad were better.
E. Budget and revenue generation
1. Budget Utilization The Centre utilized The Centre utilized Rs. 4169.695
Rs. 2707.11 lakhs lakhs which included plan and non-
including plan and non- plan funds, out of the released
plan funds out of the amount of Rs. 4226.76 lakhs (98.65
released amount of Rs. per cent utilization).
2701.33 lakhs (full
utilization of budget).
About 1.35 per cent of the released amount (mainly under establishment – salary and pension)
was not utilized during the current quiquennium as against full utilization in the previous.
2. Revenue Generation 222.0 326.41
(lakh Rs.)
QRT Comments
There has been 47 per cent increase in revenue generation. The increase was mainly due to
consultancy services and contractual research. There is need to generate more income on the
supply of plant material.


4. Summary of recommendations
4.1 Research
4.1.1. Germplasm management
 Efforts made to introduce genotypes resulted in a limited success in the past due to
many reasons. Hence efforts may be intensified to widen the genetic base by raising
self-pollinated and open pollinated seedlings.
 Presently, the germplasm contains only limited number of rootstocks and entire Indian
grape industry rely mainly on Dogridge and to some extent 110R. It is important to
study the root system of other rootstocks and evaluate them for their suitability under
different conditions of soil biotic and abiotic stresses and affinity to nutrients.
4.1.2. Genetic Improvement
 Conventional breeding including rootstock breeding must be intensified supported by
embryo rescue and Marker Assisted Selection (MAS). ICAR may consider providing
additional land, manpower and budget.
 Hitherto crosses were made involving varieties with desirable traits and hybrids were
identified and released ascribing and highlighting certain favorable traits; but none of
them could replace the existing varieties commercially. Hence selection of parents
should be made with specific objectives and their progeny needs to be assessed
comprehensively for all useful traits in addition to the trait for which they are bred.
 Emphasis should be given to breeding for naturally loose bunch and bold berries. Under
breeding for uniform colour development, colour retention should be one of the
objectives. Breeding for resistance should focus on gene pyramiding for powdery and
downy mildew. Embryo rescue and marker assisted selection must be used for
accelerating the breeding.
 Data generated through RNA sequencing of different abiotic stresses must be analyzed
further to identify the critical proteins which are suppressed during the stress. This
information will be useful for developing the cultural practices to revive the function
of these proteins even under stress.
 Gene editing techniques like CRISPR/CAS should be explored and integrated in
ongoing breeding programs especially for disease resistance breeding.
 The genetic basis for chloride exclusion by the roots of Dogridge and less preference
of 110R roots for Na+ ions needs to be identified.
4.1.3. Development and refinement of production technologies
 To increase the popularity of varieties with proven traits which are either developed by
institute and farmers or introduced, package of practices should be developed.
 With increasing area under grape cultivation and majority of pruning during October
leads to glut in the market thereby, affecting returns. Off-season production under
plastic cover needs to be perfected to expand the window in international market for
Indian grapes and improve their profitability.
 For efficient fertilizer management, there is a need to promote secondary and
micronutrients to increase efficiency of major nutrients. Therefore, the research on
micronutrients with particular reference to nano particles and enzyme activity as the
indicator may be taken up.
 Emphasis should be towards developing Artificial Intelligence tools for diagnosing the
moisture stress, nutrient disorders, pest and disease incidence and rectify the same by
tailored sprays by drones, as a means of precision viticulture.

 In canopy management studies, ideotype canopy architecture with optimum cordon
length and leaf area should be standardized for important commercial varieties. Canopy
architecture and training system may be modeled so as to allow mechanization of farm
operations.
 The Centre has released the hybrid Manjari Medika for juice purpose. Its juice, skin
and seed were found to have high antioxidant and anti-carcinogenic properties. Hence
its juice could be promoted as a healthy drink rather than just juice. For its grapes to be
available for a longer period in an year and facilitate the factory running for a long time,
scope of its double cropping be explored in Tamil Nadu and around Bengaluru for year
round production under cover.
 A virus free scion block of commercially important varieties and rootstocks should be
established. Advance techniques of propagation and virus detection may be used for
this purpose. Monitoring of soil health (biological, physical and chemical indicators) to
know the key soil indicators limiting yield of grapevines and create a more favourable
soil environment for sustainable production.
 Threshold levels of tolerance to abiotic stresses by different stionic combinations
should be determined.
 Evaluation and quantification of nutrient replacement values for different sources of
organic manures and biofertilizers for integrated nutrient management in vineyards.
4.1.4. Development and refinement of integrated protection technologies
 Biochemical basis of induced systemic resistance may be studied. Similarly the mode
of action of antagonists should be established. Duration of effectiveness of
Trichoderma should be substantiated with the data.
 A survey may be taken up to assess the impact of bacterial leaf spot on yield. Alternate
 Bacterial isolates from the honeydew of mealybugs for their attraction and effect on
systematic and focused approach to identify volatiles and to plan further applied
research.
 Bio-intensive strategies for insect and disease management may be strengthened to
produce pesticide residue free grapes. Work on machine learning and artificial
intelligence for insect detection and management is good initiative and should be
intensified.
 Alert towards the occurrence of new pests such as Fall armyworm is highly essential
and anticipatory research in this regard is suggested.
 In case of Stromatium barbatum, preference for stem parts may be studied. Strategy
may be developed to use the deadwood for attracting the females and subsequently
killing them. Based on the preliminary observation of attraction of stem borer towards
dead wood, research on Apneumones (semiochemicals) concept should be explored.
 Research on nano-pesticides against insect pests may be taken up.
4.1.5. Pre- and post-harvest technologies
 Stability of the aroma compounds produced by β-glucosidase enzyme positive yeast
strains should be studied with specific duration. Attempts should be made to
commercialize these strains.
 Adding coarse pomace for making cookies should be studied in detail considering the
quality parameters including few rheological parameters also. Development of these
value added products should aim as a health foods.

Since raisin industry in the country is expanding, the institute should give more
emphasis on developing technologies for grape drying including raw material, drying
process, packaging, minimizing browning during storage etc.
 Phenolic compounds obtained from grapes should be encapsulated and used for the
development of value added products and health foods.
4.1.6. Food safety
 Assessment of pesticide residues in market raisin samples should continue in future.
 Simple to use Radio-immuno based assay method for aflatoxins may be developed.
4.2 Transfer of Technology
 Decision Support System; IPM informatics (by the use of ICT), Village Knowledge
Centres (VKCs) and growers’ clubs need to be established on pilot basis to enable
virtual extension and research communication.
 Extension activities of the Centre must be extended to Karnataka and Tamil Nadu also
to fulfil its national mandate.
 The Centre should involve ATARI / KVKs located in major grape growing districts of
Maharashtra.
4.3 Linkages
 International research collaboration may be established with research institutes working
on tropical enology to further the research on β-glucosidase enzyme positive yeasts to
improve wine quality with proper understanding of IPR issues.
 Explore the possibility of collaboration with Mahatma Gandhi Institute for Rural
Industrialization, Wardha for research on raisins as they have big facility for solar
drying.
 To increase the visibility at international fora, it is important that Institute actively
participate in the meetings of international bodies. QRT is informed that presently India
is not a member of OIV, an important international organization related to grape and
grape processing. QRT strongly recommends that India should become a member of
OIV.
4.4 Management
 Additional land is required for expansion of nursery activities, establishment of hybrid
evaluation block and new laboratories. It is recommended that 4.0 ha of land of
ICAR-NRC Grapes given to Directorate of Onion and Garlic Research may be given
back to the Centre to fulfill the needs of immediate future.
 It is recommended that two posts of junior level scientist each in the disciplines of Plant
Breeding and Horticulture; one each in Biotechnology, Plant physiology, Entomology,
Food Technology may be created. Accordingly the number of technical posts be
sanctioned. Approval may be accorded by the council at the earliest for filling the
vacant posts in the administrative cadre.
 In view of the necessary intensification of research to support the fast developing grape
industry, the Centre may be upgraded to the level of an Institute.
 Scientists and technical staff working in laboratories need more exposure to the state of
art research techniques in plant breeding, bio-fortification, plant nutrition, eco-
physiology, tracer techniques, nano-technology and environment protection.


Annexure 1
List of projects
S. No. Title Start date End date
I. Conservation, characterization and utilization of grape
1. Management of grape genetic resources of table, wine, raisin, juice and 01/10/2008 30/09/2018
rootstock varieties - Phase II
2. Clonal selection in grapes 01/04/2011 29/02/2016
3. Validation of DUS descriptors for Indian grapes (Vitis spp.) (PPVFRA 01/04/2012 31/03/2019
funded)
4. Characterization, regeneration and documentation of grape germplasm 01/10/2015 30/06/2017
5. Consortium Research Platform on Agrobiodiversity 01/10/2015 30/09/2017
(ICAR-CRP funded)
II. Genetic improvement of grape
6. Breeding for Downy Mildew Resistance in Seedless Grape Varieties 20/09/2010 31/03/2020
7. Proteomic Analysis of Thompson Seedless Grapes Grafted on Different 29/04/2011 26/12/2014
Rootstocks at Different Phenological Stages of Growth and Development
8. In Silico Identification Of Abiotic Stress (Salinity) Responsive 01/01/2012 31/03/2014
Transcription Factors And Their Cis-Regulatory Elements In Grape
9. Functional analysis of salinity stress response in grapevine (DBT 01/01/2013 31/03/2016
funded)
10. Understanding rachis and berry elongation in response to GA3 01/01/2013 31/01/2016
application in Thompson Seedless grapes using functional genomics
approach (DBT funded)
11. Breeding for naturally loose bunches and bold berries in grapes 01/12/2014 30/11/2019
12. An integrated approach of molecular breeding for downy and powdery 21/05/2015 20/05/2018
mildew resistance in grape (DBT funded)
13. Functional validation and expression assay of abiotic stress responsive 01/04/2016 31/03/2019
transcription factors genes in grapevine
14. Creating gene and ploidy variations for desired trait in grape using 07/04/2016 31/03/2021
physical and chemical agents
15. Genetic improvement of coloured grapes. 01/09/2017 31/12/2022
III Development and refinement of production technologies for enhancing quality, productivity
and sustainability in grape
16. Standardization of cultural practices to increase quality yield of wine 01/04/2010 30/03/2017
grapes
17. Standardization of wine varieties for yield and quality wine under Indian 01/04/2011 31/03/2018
condition
18. Evaluation of rootstocks for growth, yield, fruit composition and wine 01/01/2012 30/06/2017
quality of Cabernet Sauvignon grapes grown in Pune region of India
19. Evaluation of rootstocks for growth, yield and fruit composition of table 01/04/2015 31/03/2020
and wine grapes
20. Standardization of protocol for micro-propagation of grape (Vitis vinifera 30/04/2015 30/04/2017
L.) rootstocks

21. Development of tissue culture techniques for production of quality 01/04/2017 31/03/2019
planting material in grape
22. Techniques to Improve Nutrient Use Efficiency Including Farm Waste 01/04/2007 31/08/2016
23. Developing Petiole Nutrient Guides for Cabernet Sauvignon Grapes 02/04/2008 30/06/2014
24. Standardizing Irrigation Schedule for Cabernet Sauvignon Vines Raised 01/04/2010 31/07/2014
on 110R Rootstock
25. Decision support system for enhancing productivity of grapes under 01/06/2012 30/06/2016
moisture and temperature stress conditions (NASF funded)
26. Standardizing irrigation schedule for Fantasy Seedless vines raised on 05/04/2013 31/07/2017
110R rootstock
27. Use of remote sensing for precision farming – case study for selected 30/12/2015 30/09/2017
grape vineyards in Nasik (MNCFC funded)
28. To demonstrate techniques to improve water use efficiency in growers’ 01/04/2015 31/07/2021
field
29. Effect of plastic cover on grapevine growth and productivity 01/09/2016 30/09/2019
30. Physiological disorders and their management practices in grapes 01/10/2011 30/09/2017
31. Standardization of growth regulator schedule for new grape varieties 01/10/2012 30/06/2016
32. Studies towards enhancement of source sink relationship by 14C/ 01/06/2013 31/05/2016
gibberellin as radiotracer (BRNS-BARC funded)
33. Studies on usefulness of CCC for fruitfulness, its dissipation and fate in
grapes
34. Climate based spatial delimitation of suitable grape growing regions in 01/04/2015 31/03/2019
India using GIS
IV Development and refinement of integrated protection technologies in grape
35. Development and Testing of Disease Forecasting Models and 01/04/2007 31/03/2014
Development of Pest Alert Systems
36. Studies on Bio-Efficacy of Fungicides and Safer Environmental Profiles 01/04/2007 31/03/2014
Products for Management of Grape Diseases
37. Studies on Virus Diseases of Grapes 01/04/2007 31/03/2014
38. Isolation, bio-efficacy evaluation, characterization and formulation of 01/01/2006 31/03/2014
viticulturally important micro-organisms
39. ORP on 'Diagnosis and management of leaf spot diseases of field and 25/06/2009 30/03/2014
horticultural crops' – grapes
40. Studies on the biology and control of the fungi causing anthracnose 01/06/2009 31/12/2014
disease in grapes
41. A holistic analysis for the absence of Botrytis cinerea infections in grapes 01/01/2012 30/06/2014
42. Development of microbial formulations for biological control of grape 01/04/2014 31/03/2017
diseases (AMAAS funded)
43. Studies on bacterial leaf spot and its management in grapes 01/07/2015 30/06/2018
44. Monitoring of fungicide resistance in natural field populations of 01/01/2016 31/03/2017
Plasmaopara viticola and Erysiphe necator in commercial grape
vineyards and developing mitigating strategies (Extramural)

45. Bio-intensive disease and pest management module for production of 01/04/2017 31/03/2020
residue compliant quality grapes(AMAAS partially funded)
46. Insect biodiversity in grapevine ecosystem with emphasis on 01/12/2010 31/03/2016
economically important grape pests
47. Multi-pronged strategy for the management of Mealybugs in grapes 01/04/2012 31/03/2017
48. Management of stem borer in grapes 01/04/2013 31/03/2019
49. Consortium Research Platform on Borers in Network Mode (ICAR CRP 01/09/2014 31/03/2017
funded)
50. Out reach programme on management of sucking pests (ICAR ORP 08/04/2015 31/12/2017
funded)
51. Detection and management of biotic and abiotic stresses in vineyards
using artificial intelligence based wearable device
V. Development of pre- and post-harvest technologies for processing of grapes and value
addition
52. Profiling of grape varieties Shiraz and Sauvignon Blanc for its phenolic 01/07/2012 31/07/2015
and aroma compounds from grape to wine
53. Identification and evaluation of β glucosidase producing yeast strains and 01/10/2013 30/06/2016
its impact on wine quality
54. Winery By-products Utilization for Value Addition in Food Products 01/01/2014 31/12/2018
55. Phytochemical profiling and development of nutraceuticals and value 01/04/2016 31/03/2021
added products from grapes
56. Studies of pre-harvest and drying conditions to improve quality of raisins 01/10/2015 30/09/2018
VI Food safety in grapes and its processed products
57. Monitoring of Agrochemical Residues in Grape and Grape Produce 01/04/2008 31/03/2014
58. Persistence Studies of Agrochemical Residues in Soil and Water 01/04/2008 31/03/2014
59. Studies on Dissipation Rate of New Generation Pesticides with Reference 01/04/2008 31/03/2014
to Changing MRLs
60. Comprehensive screening of target, non-target and unknown emerging 19/09/2014 30/06/2016
organic contaminants in fruits and vegetables by GC/MS and LC/MS
(ICAR National Fellow project)
61. Analysis and safety evaluation of agrochemical residues and 01/01/2014 31/12/2018
contaminants in agricultural commodities and processed products
62. Nutritional quality and safety evaluation of common processed products 01/12/2016 30/11/2018
of grape (FSSAI funded)
VII. Other
63. NRCG-DIPS-A system for diagnosis and management of important 01/05/2008 30/04/2014
diseases and insect pests of grapes
64. National Referral Laboratory for Monitoring Pesticide Residues for 01/04/2012 30/06/2018
Export of Fresh Grapes from India (APEDA funded)
65. Intellectual Property Management and Transfer / Commercialization of 01/04/2012 31/03/2020
Agricultural Technology (NAIP funded)
66. Cultivation of Commercial Seedless Varieties of Grapes at Taldangra 01/09/2017 31/08/2020
Horticulture R & D Farms of Bankura District, West Bengal (State Govt.
of West Bengal funded)

Annexure 2
List of externally funded projects
S. Title Period Funding Cost
No. Source (₹ lakhs)
1. Validation of DUS descriptors for Indian grapes (Vitis 01/04/2012 to PPVFRA 25.00
spp.) 31/03/2019
2. Consortium Research Platform on Agrobiodiversity 01/10/2015 to ICAR-CRP 12.20
30/09/2017
3. Functional analysis of salinity stress response in 01/01/2013 to DBT 95.56
grapevine 31/03/2016
4. Understanding rachis and berry elongation in response 01/01/2013 to DBT 66.572
to GA3 application in Thompson Seedless grapes using 31/01/2016
functional genomics approach
5. An integrated approach of molecular breeding for 21/05/2015 to DBT 41.66
downy and powdery mildew resistance in grape 20/05/2018
6. Decision support system for enhancing productivity of 01/06/2012 to NASF 109.76
grapes under moisture and temperature stress conditions 30/06/2016
7. Use of remote sensing for precision farming – case 30/12/2015 to MNCFC 14.06
study for selected grape vineyards in Nasik 30/09/2017
8. Studies towards enhancement of source sink 01/06/2013 to BRNS- 24.00
relationship by 14C/ gibberellin as radiotracer 31/05/2016 BARC
9. Development of microbial formulations for biological 01/04/2014 to AMAAS 50.00
control of grape diseases 31/03/2017
10. Monitoring of fungicide resistance in natural field 01/01/2016 to Extramural 60.00
populations of Plasmaopara viticola and Erysiphe 31/03/2017
necator in commercial grape vineyards and developing
mitigating strategies
11. Bio-intensive disease and pest management module for 01/04/2017 to AMAAS 7.91
production of residue compliant quality grapes 31/03/2020
12. Consortium Research Platform on Borers in Network 01/09/2014 to ICAR CRP 28.20
Mode 31/03/2017
13. Out reach programme on management of sucking pests 08/04/2015 to ICAR ORP 15.00
31/12/2017
14. Comprehensive screening of target, non-target and 19/09/2014 to ICAR 131.06
unknown emerging organic contaminants in fruits and 30/06/2016 National
vegetables by GC/MS and LC/MS Fellow
15. Nutritional quality and safety evaluation of common 01/12/2016 to FSSAI 30.744
processed products of grape 30/11/2018
16. National Referral Laboratory for Monitoring Pesticide 01/04/2012 to APEDA 3586.22
Residues for Export of Fresh Grapes from India 30/06/2018
17. Intellectual Property Management and Transfer / 01/04/2012 to NAIP
Commercialization of Agricultural Technology 31/03/2020
18. Cultivation of Commercial Seedless Varieties of Grapes 01/09/2017 to State Govt. of 49.00
at Taldangra Horticulture R & D Farms of Bankura 31/08/2020 West Bengal
District, West Bengal


Annexure 3
Publications
Year wise details of research papers
Items 2013-14 2014-15 2015-16 2016-17 2017-18 Total

Research papers 30 22 23 31 25 131
In international journals 16 9 13 16 13 67
In national journals 14 13 10 15 12 64
NAAS score  6.0 15 10 19 16 7 67
List of research papers

2013-14
1. Ahammed Shabeer T.P., Saha Ajoy, Gajbhiye V.T., and Gupta Suman. 2013. Optimization
and validation of LLE/HPLC-DAD method to determine the residues of selected PAHs in
surface water. International Journal of Agriculture, Environment & Biotechnology. 6(2):
241-248.
2. Amala U. and Yadav D.S. 2013. Influence of natural and artificial diets over the biological
parameters of acarophagous predatory beetle, Stethorus rani Kapur. Pest Management for
Horticultural Ecosystems. 19(2): 169-172.
3. Amala U. and Yadav D.S. 2013. Study on life table parameters and predatory potential of
Stethorus rani Kapur on red spider mite, Tetranychus urticae Koch, Biopesticides
International. 9(2): 113–119.
4. Amala U., Yadav D.S. and Bhosale A.M. 2013. Studies on Parasitoid complex of mealybug
infesting grapes in Maharashtra. Journal of Applied Horticulture. 15(2): 117-119.
5. Goswami A.K., Somkuwar R.G., Samarth Roshni R., Sharma A.K., Nawale Supriya, and
Itroutwar Prerna. 2013. Evaluation of coloured seedless table grape varieties for increase
in shelf-life. HortFlora Research Spectrum. 2(40): 324-328.
6. Jadhav M.R., Utture S.C., Banerjee Kaushik, Oulkar D.P., Sabale R. and Ahammed
Shabeer TP. 2013. Validation of a residue analysis method for streptomycin and
tetracycline and their food safety evaluation in pomegranate (Punica granatum L.). J.
Agric. Food Chem. 61(36): 8491-8498.
7. Mazumdar M., Das S., Saha U., Chatterjee M., Banerjee Kaushik, Basu D. 2013. Salicylic
acid-mediated establishment of the compatibility between Alternaria brassicicola and
Brassica juncea is mitigated by abscisic acid in Sinapis alba. Plant Physiology
Biochemistry. 70: 43-51.
8. Mujawar S., Utture S.C., Fonesca E., Matarrita J., Banerjee Kaushik. 2013. Validation of a
GC-MS method for the estimation of dithiocarbamate fungicide residues and safety
evaluation of mancozeb in fruits and vegetables. Food Chemistry. 150: 175-181.
9. Nagarajan G., Khan Z., Utture S.C., Dasgupta S., Banerjee Kaushik. 2013. Ensuring
selectivity and sensitivity by timed- and ultra-selective reaction monitoring during gas
chromatography tandem mass spectrometric determination of pesticides. Journal of
Chromatography A. 1318:226-233.

10. Ramteke S.D., Bhange M.A., Somkuwar R.G., and Kor R.J. 2012. Efficiency of weedicide
(UPH 707) to control complex weed flora in Thompson Seedless grape vineyard.
Progressive Horticulture. 45 (2): 259-264.
11. Ramteke S.D., Rajurkar A.B., Bhange M.A., and Kor R.J. 2012. Chemical management of
broad leaved weeds in grapes. Indian Journal of Weed Science. 44(3): 198–202.
12. Sabale R., Ahammed Shabeer T.P., and Banerjee Kaushik. 2013. Dissipation kinetics,
safety evaluation and assessment of pre-harvest interval (PHI) and processing factor for
kresoxim methyl residues in grape. Environmental Monitoring and Assessment. 186 (4):
2369-2374.
13. Saha S., Banerjee Kaushik, and Rai A.B. 2013. Bioefficacy, residue dynamics and safety
assessment of the combination fungicide trifloxystrobin 25% + tebuconazole 50% 75 WG
in managing early blight of tomato (Lycopersicon esculentum Mill.). Journal of
Environmental Science and Health. Part B 49: 134-141.
14. Salunkhe Varsha P., Sawant Indu S., Banerjee Kaushik, Rajguru Yogita R., Wadkar Pallavi
N., Oulkar D.P., Naik D.G., and Sawant S.D. 2013. Biodegradation of profenophos by
Bacillus subtilis isolated from grapevines. Journal of Agricultural Food Chemistry. 61:
7195-7202.
15. Satisha J., Keith R Striegler, Eli Bergmeier, and Jackie Harris. 2013. Influence of canopy
management practices on canopy characteristics, yield and fruit composition of Norton
grapes (Vitis aestivalis Michx). International Journal of Fruit Science. 13: 441-458.
16. Satisha J., Oulkar D.P., Banerjee Kaushik, Sharma J., Patil A.G., Maske S.R., Somkuwar
R.G. 2013. Biochemically induced variations during some phonological stages in
Thompson Seedless grapevines grafted on different rootstocks. South African Journal of
Enology and Viticulture. 34 (1): 36-45.
17. Satisha J., Oulkar D.P., Vijapure Amruta N., Maske Smita R., Sharma A.K., and Somkuwar
R.G. 2013. Influence of canopy management practices on fruit composition of wine grape
cultivars grown in semiarid tropical region of India. African Journal of Agricultural
Research. 8 (24): 3462-3472. DOI. 10.5897/AJAR/ 12.2066.
18. Satisha J., Ramteke S.D., Sharma Jagdev, and Upadhyay A.K. 2014. Moisture and salinity
stress induced changes in biochemical constituents and water relations of different grape
rootstock cultivars. International Journal of Agronomy, pp: 1-8. DOI:
http://dx.doi.org/10.1155/2014/789087.
19. Satisha J., Sharma A.K., and Adsule P.G. 2014. Rootstock influence on the biochemical
composition and polyphenol oxidase activity of ‘Thompson Seedless’ grapes and raisins.
International Journal of Fruit Science, 14(2): 133-146. DOI:
10.1080/15538362.2013.817767.
20. Sawant Indu S., Shetty D.S., Narkar Shubhangi P., Ghule S., and Sawant S.D. 2013.
Climate change and shifts in etiology of anthracnose disease of grapevines in India. Journal
of Agrometeorology. 15: 75-78.
21. Sharma AK, Kumar R, Azad ZRAA and Adsule PG. 2013. Use of fine wine lees for value
addition in ice cream. Journal of Food Science and Technology. 52(1):592–596. (NAAS:
8.20).
22. Shinde Manisha P., Upadhyay Anuradha, Aher Lalitkumar B. and Karibasappa G.S. 2013.
Molecular marker analysis to differentiate a clonal selection of Centennial Seedless
grapevine. African J. of Biotechnology. 12(14):1594-1597.

23. Siddharth and Sharma A.K. 2013. Blending of Kokum extract in Concord grape juice:
Study on physico-chemical characteristics, sensory quality and storage. International
Journal of Food and Fermentation Technology. 3(1): 79-83.
24. Somkuwar R.G., Bondage D.D., Surange M., Navale S., and Sharma A.K. 2013. Yield,
raisin recovery and biochemical characters of fresh and dried grapes (raisin) of Thompson
Seedless grapes (Vitis vinifera) as influenced by different rootstocks. Indian Journal of
Agricultural Sciences. 83(9): 924-927.
25. Somkuwar R.G., Samarth Roshni R., Satisha J., Ramteke S.D, and Itroutwar Prerna. 2013.
Status of dry matter at harvesting stage in commercially grown varieties under tropical
climatic condition. HortFlora Research Spectrum. 2(2): 110-115.
26. Somkuwar R.G., Satisha J., and Ramteke S.D. 2013. Berry weight, quality and biochemical
changes in relation to cane thickness of own rooted and grafted Tas-A-Ganesh grapes.
Journal of Horticultural Sciences. 18(1): 30-34.
27. Somkuwar R.G., Sharma J., Satisha J., Khan Ishrath, and Itroutwar Prerna. 2013. Effect of
zinc application to mother vines of Dogridge rootstock on rooting success and
establishment under nursery conditions. Intl. J. Scientific. Tech. Res. 2: 198-201.
28. Ugare B., Banerjee Kaushik, Ramteke S.D., Pradhan S., Oulkar D.P., Utture S.C., and
Adsule P.G. 2013. Dissipation kinetics of forchlorfenuron, 6-benzyl aminopurine,
gibberellic acid and ethephon residues in table grapes (Vitis vinifera). Food Chemistry. 141
(2013) 4208-4214.
29. Upadhyay A.K., Sharma Jagdev, and Satisha J. 2013. Influence of rootstocks on salinity
tolerance of Thompson Seedless grapevines. Journal of Applied Horticulture, 15(3): 173-
177.
30. Upadhyay Anuradha, Aher Lalithkumar B., Shinde Manisha P., Mundankar Kavita Y.,
Datre Anuj, and Karibasappa G.S. 2013. Microsatellite analysis to rationalize grape
germplasm in India and development of a molecular database. Plant genetic resources:
characterization and utilization. 11(3): 225–233.
2014-15
1. Amala U. and Yadav D.S. 2014. Life table studies of pink mealybug, Maconellicoccus
hirsutus under laboratory conditions. Indian Journal of Plant Protection 42(3): 280-282.
2. Amala U., Chinniah C., Sawant Indu S., Muthukrishnan N. and Muthiah C. 2014. Bio-
efficacy and lethal reproductive effects of three entomopathogenic fungi against pink
mealybug, Maconellicoccus hirsutus Green infesting grapes, Green Farming 5(4): 199-202.
3. Amala U., Chinniah C., Sawant Indu S., Muthukrishnan N. and Muthiah C.. 2014. Survey
for Grapevine Mealy bug Incidence and their Natural Enemies in Tamil Nadu and
Maharashtra. Bio pesticides International, 10(2): 169-175.
4. Hingmire S., Oulkar D.P., Shabeer A.T.P., Banerjee K. 2015. Residue analysis of fipronil
and difenoconazole in okra by liquid chromatography mass spectrometry and their food
safety evaluation. Food Chem. 176: 145-151.
5. Khot A.P., Ramteke S.D. and Deshmukh M.B. 2014. Influence of CPPU and GA3 on bunch,
berry characteristics and biochemical changes in relation to yield of grapes grafted on
Dogridge rootstock Ann. Plant Physiol., 28 (2): 14-21.
6. Khot A.P., Ramteke S.D.,Banerjee K. and Girame Rushali R. 2014. Dissipation and
persistence of propargite in grape leaves and berries using GC-MS. Pestology vol.
XXXVIII no. 4.
7. Ramteke S.D. and Khot A.P. 2014. Bioefficacy, phytotoxicity and terminal residue analysis
of Diuron on narrow and broad leaf weeds in grapes. Ann. Plant Physiol., 28 (2): 38-43.

8. Sabale R., Shabeer A.T.P., Utture S.C., Banerjee K., Oulkar D.P., Adsule P.G., Deshmukh,
M.B. 2015. Kresoxim methyl dissipation kinetics and its residue effect on soil extra-cellular
and intra-cellular enzymatic activity in four different soils of India. J. Environ. Sci. Health,
Part B. 50: 90-98.
9. Sahoo S, Manjaiaha K.M., Dattaa S.C., Shabeer A. T.P. and Kumar J. 2014. Kinetics of
metribuzin release from bentonite-polymer composites in water. J. Environ. Sci. Health,
Part B., 49, 591–600.
10. Salunkhe V.P., Sawant I.S., Banerjee K., Wadkar P.N., Sawant S.D., Hingmire S. 2014.
Kinetics of degradation of carbendazim by B. subtilis strains: possibility of in situ
detoxification. Environ. Monit. Assess. 186 (12): 8599-8610.
11. Satisha J., Upadhyay Anuradha and Maske S.R. 2014. Rootstocks induced changes in
enzyme activity and other biochemical components during bud burst in Thompson Seedless
grapevine buds. Vitis, 53 (2): 57-64.
12. Shabeer A.T.P., Banerjee K., Jadhav M., Girame R., Utture S.C., Hingmire S., Oulkar S.P.
2015. Residue dissipation and processing factor for dimethomorph, famoxadone and
cymoxanil during raisin preparation. Food Chem. 170: 180-185.
13. Shabeer A.T.P., Saha A, Gajbhiye V.T., Gupta S, K.M. Manjaiah K.M. and Varghese E.
2014. Removal of Poly Aromatic Hydrocarbons (PAHs) from Water: Effect of Nano and
Modified Nano-clays as a Flocculation Aid and Adsorbent in Coagulation flocculation
Process. Polycyclic Aromatic Compounds, 34:452–467.
14. Sharma A.K., Kumar R., Azad ZRAA and Adsule P.G. 2015. Use of fine wine lees for
value addition in ice cream. Journal of Food Science and Technology. 52(1):592–596.
15. Shetty D.S., Narkar S.P., Sawant I.S. and Sawant S.D. 2014. Efficacy of quinone outside
inhibitors (QoI) and demethylation inhibitors (DMI) fungicides against grape anthracnose.
Indian Phytopathology, 67: 174-178.
16. Somkuwar R.G., Bahetwar A., Khan I., Satisha J., Itroutwar P., Bhongale A. and Oulkar
D. 2014. Changes in growth, photosynthetic activity, biochemical parameters and amino
acid profile of Thompson Seedless grapes (Vitis vinifera L.). Journal of Environmental
Biology. 35: 1157- 1163. (NAAS 2014 6.8).
17. Somkuwar R.G., Bhange M.A., Upadhyay A.K., and Ramteke S.D. 2014. Interaction effect
of rootstocks on gas exchange parameters, biochemical changes and nutrient status in
Sauvignon Blanc wine grapes. Journal of Advances in Agriculture. Vol. 3(3): 218 – 225.
(NAAS 2014 7.4).
18. Somkuwar R.G., Ramteke S.D., Satisha J., Bhange M.A., and Itroutwar P. 2014. Effect of
canopy management practices during forward pruning on berry development and
photosynthesis in Tas-A-Ganesh grapes. Journal of Horticultural Science. 9(1): 18-22.
(NAAS 2014 3.7).
19. Somkuwar R.G., Samarth R.R., Itroutwar P. and Navale S. 2014. Effect of cluster thinning
on bunch yield, berry quality and biochemical changes in local clone of table grapes cv.
Jumbo Seedless (Nana Purple). Indian Journal of Horticulture. 71 (2): 184-189. (NAAS
2014 6.11).
20. Somkuwar R.G., Samarth Roshni R., Satisha J., Ramteke S.D. and Itroutwar Prerna. 2014.
Status of dry matter at harvesting stage in commercially grown grape varieties under
tropical climatic condition. J. Agric. Res. Technol., 39 (3): 407-412.
21. Somkuwar R.G., Satisha J., Sawant S.D., Taware P.B., Bondage D.D. and Itroutwar P.
2014. Rootstocks influence the growth, Biochemical contents and Disease Incidence in

Thompson Seedless Grapevines. British Journal of Applied Science and Technology 4(6):
1030- 1041. (NAAS: 5.32)
22. Somkuwar R.G., Sharma J., Satisha J., Ramteke S.D., Khan I and Itroutwar P 2014.
Growth, fruitfulness and yield in relation to mycorrhizal inoculants in Thompson Seedless
grapes (Vitis vinifera L.) grafted on Dog Ridge rootstock. Progressive Horticulture. 46 (1)
23-30. (NAAS 2014 3.25)
2015-16
1. Amala U, Chinniah C, Sawant IS, Muthukrishnan N. and Muthiah C. 2015. Safety
evaluation of Spirotetramat 150 OD against predator Chrysoperla zastrowi sillemi (Esben
Peterson) (Neuroptera: Chrysopidae) under laboratory conditions. Journal of Biopesticides
8(1): 52-55. (NAAS: 3.88)
2. Amala U, Chinniah C, Sawant IS, Yadav DS, Phad DM. 2016. Comparative biology and
fertility parameters of two spotted spider mite, Tetranychus urticae Koch. on different
grapevine varieties. Vitis, 55: 31-36. (NAAS: 6.74)
3. Chatterjee NS, Banerjee K, Utture S, Kamble N, Rao BM, Panda SK, and Mathew S. 2015.
Assessment of polyaromatic hydrocarbons and pesticide residues in domestic and imported
Pangasius (Pangasianodon hypophthalmus) fish in India. J. Sci. Food Agric. 96(7):2373-
2377. (NAAS: 7.71)
4. Chatterjee NS, Utture S, Banerjee K, Shabeer ATP, Kamble N, Mathew S. and Kumar KA.
2016. Multiresidue analysis of multiclass pesticides and polyaromatic hydrocarbons in fatty
fish by gas chromatography tandem mass spectrometry and evaluation of matrix effect.
Food Chem. 196: 1-8. (NAAS: 9.39)
5. Dangi RS, Oulkar DP, Dhakephalkar P, Singh SK, Banerjee K, Naik D, Tamhankar S, Rao
S. 2016. Antimicrobial activity of some Trigonella species. Int. J. Phytomedicine. 8 (1):80-
94. DOI: http://dx.doi. org/10.5138/ijpm.v8i1.1782. (NAAS: 7.71)
6. Dutta MK, Sengar N, Kamble N, Banerjee K, Minhas N, Sarkar B. 2016. Image processing
based technique for classification of fish quality after cypermethrine exposure. LWT- Food
Sci. Technol. 68: 408-417. (NAAS 8.42).
7. Ghule SB, Sawant IS, Shetty DS, Sawant SD. 2015. Epidemiology and weather-based
forecasting model for anthracnose of grape under the semiarid tropical region of
Maharashtra. Journal of Agrometeorology, 17: 265-267. (NAAS: 6.16; IF 0.145)
8. Khan Z, Girame R, Utture SC, Ghosh RK, Banerjee K. 2015. Rapid and sensitive
multiresidue analysis of pesticides in tobacco using low pressure and traditional gas
chromatography tandem mass spectrometry. J. Chromatogr. A. 1418: 228-232. (NAAS
10.17)
10. Narkar SP and Sawant IS. 2016. In vitro evaluation of carbendazim resistant
Colletotrichum gloeosporioides isolates of grapes for sensitivity to QoI and DMI
fungicides. Indian Phytopathology, 69: 77-81. (NAAS: 4.59)
11. Salunkhe VP, Sawant IS, Banerjee K, Wadkar PN, Sawant SD. 2015. Enhanced dissipation
of triazole and multi-class pesticide residues on grapes after foliar application of grapevine
associated Bacillus species. Journal of Agricultural and Food Chemistry, 63: 10736-10746.
(NAAS: 9.11; IF 2.912)
12. Satisha J, Kitture AR, Sharma AK, Sharma J, Upadhyay AK and Somkuwar RG. 2015.
Regulation of fruit and wine quality parameters of Cabernet Sauvignon grapevines (Vitis
vinifera L.) by rootstocks in semiarid regions of India. Vitis, 54: 65-72. (NAAS: 6.74)

13. Satisha J, Upadhyay A, Maske SR and Shinde MP. 2015. A protocol for protein extraction
from recalcitrant tissues of grapevine (Vitis vinifera L) for proteome analysis. Indian
Journal of Biotechnology 14(4): 532-539. (NAAS: 6.39).
14. Sawant IS, Ghule SB, Sawant SD. 2015. Molecular analysis reveals that lack of
chasmothecia formation in Erysiphe necator in Maharashtra, India is due to presence of
only MAT1-2 mating type idiomorph. Vitis, 54: 87–90. (NAAS: 6.79; IF 0.738)
15. Sawant SD, Ghule MR, Sawant IS. 2016. First Report of QoI Resistance in Plasmopara
viticola from Vineyards of Maharashtra, India. Plant Disease. 100: 229 (NAAS 8.74; IF
3.02).
16. Shabeer ATP, Girame R, Hingmire S, Banerjee K, Sharma AK, Oulkar D, Utture S,
Jadhav M. 2015. Dissipation pattern, safety evaluation, and generation of processing factor
(PF) for pyraclostrobin and metiram residues in grapes during raisin preparation.
Environmental Monitoring and Assessment, 187:31. (NAAS: 7.68)
17. Sharma AK, Banerjee K, Ramteke SD, Satisha J, Somkuwar RG and Adsule PG. 2015.
Evaluation of Ascorbic Acid and Sodium Metabisulfite Applications for Improvement in
Raisin Quality. Proceedings of the National Academy of Sciences, India Section B:
Biological Sciences, 86(3):637-641. (NAAS: 6.0)
18. Shetty DS, Sawant IS, Narkar SP, Ghule SB, Satisha J, Karibasappa GS. 2015. Screening
of grape genotypes to identify sources of resistance to anthracnose disease and identifying
biochemical marker associated with resistance. Indian Phytopathology. 68(4): 424-431.
(NAAS 4.59)
19. Somkuwar RG, Samarth RR, Satisha J, Ramteke SD and Sharma AK. 2015. Effect of sun
exposure on berry development and biochemical constituent in Tas-A-Ganesh grapes
grafted on Dog Ridge rootstock. Progressive Horticulture, 47(1): 77-81. (NAAS: 3.25).
20. Somkuwar RG, Taware PB, Bhange MA, Sharma J and Khan I. 2015. Influence of different
rootstocks on growth, photosynthesis, biochemical composition, and nutrient contents in
‘Fantasy Seedless’ grapes. International Journal of Fruit Science, pp. 1–16.
21. Somkuwar RG, Taware PB, and Bhange MA. 2015. Rooting behavior and biochemical
changes in relation to IBA concentrations in different grape rootstocks. Indian J. Hort.
72(2): 173-177. (NAAS: 6.13)
22. Upadhyay A, Satisha J, Maske SR, Kadoo NR and Gupta VS. 2015. Expression of stable
reference genes and expression analysis of SPINDLY gene in response to gibberellic acid
application at different reproductive stages in grape. Biologia Plantarum 59: 436-444.
(NAAS: 7.86)
23. Yadav DS, Shinde AH, Bhosale AM and Jadhav AR. 2016. Differential sensitivity of
insecticides for targeting of multiple pests in grapes (Vitis vinifera). Indian Journal of
Agricultural Sciences 86 (2), 234-41. (NAAS: 6.14).
2016-17
1. Anupa T., Leela Sahijram, Samarth RR and B. Madhusudhana Rao. 2016. In vitro shoot
induction of three grape (vitis vinifera L.) varieties using nodal and axillary explants. The
Bioscan,11(1): 201-204. (NAAS: 5.26)
2. Dutta MK, Sengar N, Minhas N, Sarkar B, Goon A, and Banerjee K. 2016. Image
processing based classification of grapes after pesticide exposure. LWT- Food Sci.
Technol. 72: 368-376. (NAAS: 8.416)
3. Chatterjee NS, Utture S, Banerjee K, Shabeer ATP, Kamble N, Panda SK, Mathew S. 2016.
Multiresidue analysis of multiclass pesticides and polyaromatic hydrocarbons in fatty fish

by gas chromatography tandem mass spectrometry and evaluation of matrix effect. Food
Chemistry, 196: 1-8.
4. Ghosh S, Gurav SP, Harke AN, Chako MJ, Joshi KA, Dhepe A, Charolkar C, Shinde V,
Kitture R, Parihar VS, Banerjee K, Kamble N, Bellare J, Chopade BA. 2016. Dioscorea
oppositifolia mediated synthesis of gold and silver nanoparticles with catalytic activity. J.
Nanomedicine & Nanotehnology DOI:10.4172/2157-7439.1000398. (NAAS: 6.86)
5. Ghosh S, Harke AN, Chacko MJ, Gurav SP, Joshi KA, Dhepe A, Dewle A, Tomar GB,
Kitture R, Parihar VS, Banerjee K, Kamble N, Bellare J, Chopade BA. 2016. Gloriosa
superba mediated synthesis of silver and gold nanoparticles for anticancer applications. J.
Nanomedicine & Nanotehnology DOI:10.4172/2157-7439.1000390. (NAAS: 6.86)
6. Ghosh S, Harke AN, Chacko MJ, Gurav SP, Joshi KA, Dhepe A, Dewle A, Tomar GB,
Kitture R, Parihar VS, Banerjee K, Kamble N, Bellare J, Chopade BA. 2016. Barleria
prionitis leaf mediated synthesis of silver and gold nanocatalysts. J. Nanomedicine &
Nanotehnology DOI: 10.4172/2157-7439.1000394. (NAAS: 6.86)
7. Jadhav MR, Shabeer ATP, Nakade M, Gadgil M, Oulkar DP, Arimboor R, Menon R,
Banerjee K. 2017. Multiresidue method for targeted screening of pesticide residues in spice
cardamom (Elettariacardamomum) by Liquid Chromatography with Tandem Mass
Spectrometry. Journal of AOAC International,100(3): published online.
8. Jadhav RS, Yadav DS, Amala U, Ghule S and Sawant IS. 2015. Morphological, biological
and molecular description of Spodoptera litura infesting grapevines in tropical climate of
Maharashtra, India. Current Biotica,9(3):207-220. (NAAS (2015) : 3.68)
9. Jadhav RS, Yadav DS, Amala U, Sawant IS, Ghule SB and Bhosale AM. 2017.
Morphometric analysis and deoxyribonucleic acid barcoding of new grapevine pest,
Stromatium barbatum (Fabricius) (Coleoptera: Cerambycidae) in India. Proceedings of the
National Academy of Sciences India Section B: Biological Sciences, doi:10.1007/s40011-
017-0848-x) (NAAS (2017): 5.00)
10. Kamble AK, Sawant SD, Saha S, Sawant IS. 2017. Screening of grapevine germplasm to
identify sources of resistance to bacterial leaf spot causing Xanthomonas campestris pv.
Viticola, International Journal of Agriculture Innovations and Research, 5(5): 834-837
11. Kodandaram MH, Kumar YB, Banerjee K, Hingmire S, Rai AB, Singh B. 2017. Field
bioefficacy, phytotoxicity and residue dynamics of the insecticide flonicamid (50 WG) in
okra [Abelmoschusesculentus (L) Moench]. Crop Protection, 94: 13-19. (NAAS: 7.65)
12. Naik S, Somkuwar RG, Sharma AK and Sawant SD. 2016. Grape cultivation in Himachal
Pradesh is promising. Indian Horticulture 61(3):30-33.
13. Narkar SP and Sawant IS. 2016. In vitro evaluation of carbendazim resistant
Colletotrichum gloeosporioides isolates of grapes for sensitivity to QoI and DMI
fungicides. Indian Phytopathology, 69: 77-81. (NAAS: 4.59)
14. Narkar SP, Sawant IS and Shete HG. 2016. Isolation and identification of Bacillus
amyloliquefaciens strains for bio-control of grapevine anthracnose. Journal of eco-friendly
agriculture, 12: 62-66. (NAAS: 3.10)
15. Oulkar DP, Hingmire S, Goon A, Jadhav MR, Ugare B, Shabeer ATP, Banerjee K. 2017.
Optimization and validation of a residue analysis method for glyphosate, glufosinate, and
their metabolites in plant matrixes by Liquid Chromatography with Tandem Mass
Spectrometry. Journal of AOAC International, 100 (3): published online.
16. Ramteke SD, Girase PS and Babu N. 2016. Influence of growth regulator schedule on fruit
yield and quality of Manjri Naveen grape variety. The Ecoscan. Special Issue, Vol. IX:709-
713:2016.

17. Ramteke SD, Urkude V, Parhe SD, Bhagwat SR. 2017. Berry cracking; its causes and
remedies in grapes - A Review. Trends in Biosciences,10(2), Print: ISSN 0974-8431, 549-
556, 2017. NAAS: 3.94)
18. Saha S, Jadhav MR, Shabeer ATP, Banerjee K, Sharma BK, Loganathan M, Rai AB. 2016.
Safety assessment and bioefficacy of Fluopyram 20%+ Tebuconazole 20%-40 SC in chilli,
capsicum annum. Proceedings of the National Academy of Sciences, India Section B:
Biological Science,86 (2): 359-366.
19. SamarthRR, Bhosale P, Anupa T., Deore P and Gaikwad S. 2016. Morphological and
molecular characterization of different grape varieties. Research on Crops, 17(3): 517-523.
(NAAS: 5.00)
20. Sawant IS, Wadkar PN, Rajguru YR, Mhaske NH, Salunkhe VP, Sawant SD, Upadhyay
A. 2016. Biocontrol potential of two novel grapevine associated Bacillus strains for
management of anthracnose disease caused by Colletotrichum gloeosporioides. Biocontrol
Science and Technology, 26(7): 964-979. (NAAS: 6.97; IF 0.82)
21. Sawant SD, Ghule MR, Sawant IS. 2016. Occurrence of CAA fungicide resistance and
detection of G1105S mutation in Plasmopara viticola isolates from vineyards in Sangli,
Maharashtra, India. Plant Disease,http://dx.doi.org/10.1094/ PDIS-05-16-0753-PDN.
22. Sawant SD, Ghule MR, Sawardekar RM, Sawant IS and Sujoy S. 2016. Effective use of
activated potassium salt of long chain phosphorous (96%) for the control of fungicide
resistant Plasmopara viticola causing downy mildew in grapes. Indian Phytopathology,
69(4s):338-344. (NAAS rating 5.9).
23. Sawant SD, Sawardekar RM, Ghule MR, Sawant IS and Sujoy S. 2016. Evaluation of
amisulbrom 20% SC against Plasmopara viticola of grapes under in vitro and in vivo
conditions. Indian Phytopathology.69(4s):621-624. (NAAS rating 5.9)
24. Sharma AK, Somkuwar RG, Banerjee K and Satisha J. 2016. Effect of crop levels and
pruning timing on bunch and berry parameters of Cabernet Sauvignon grapes. Journal of
Agri Search3(3), 165-169. DOI: 10.21921/jas.v3i3.11378.
25. Sharma AK; Banerjee K; Ramteke SD; Satisha J; Somkuwar RG and Adsule PG. 2016.
Evaluation of ascorbic acid and sodium metabisulfite applications for improvement in
raisin quality. Proceedings of the National Academy of Sciences, India Section B:
Biological Sciences, 86(3): 637-641. DOI 10.1007/s40011-015-0499-8.
26. Shinde MP, Upadhyay A, Sarika, Mir Asif Iquebal and Upadhyay AK. 2016. Identification,
characterization and expression analysis of ERF transcription factor VviERF073 and
standardization of stable reference gene under salt stress in grape. Vitis,55 (4), 165-171.
27. Som Sukanya, Burman R Roy, Sangeetha V, Lenin V, Sharma JP, Banerjee K, Sawant IS.
2016. Institutional role on promotion of good agricultural practices (GAP) and export of
grapes. Journal of Community Mobilization and Sustainable Development, 11: 229-235.
(NAAS: 5.30)
28. Somkuwar RG, Bhange M, Sharma J, Upadhyay AK, and Khan I. 2017. Interaction of
biochemical and nutritional status of nodal sections with rooting success in grape
rootstocks. J. Environ. Biol.38(1): 115-121.
29. Swami S, Mishra SR, Saha S, Kaur C, Shabeer ATP, Oulkar DP, Banerjee K, Singh N,
Singh SB. 2016. Ozonation for pesticide residue removal and its effect on ascorbic acid,
lycopene, β-Carotene and phenolic content in tomato (Lycopersiconesculentum) fruits.
Pesticide Research Journal, 28 (1): 42-51.
30. Swami S, Muzammil R, Saha S, Shabeer ATP, Oulkar DP, Banerjee K, Singh SB. 2016.
Evaluation of ozonation technique for pesticide residue removal and its effect on ascorbic

acid, cyanidin-3-glucoside, and polyphenols in apple (Malusdomesticus) fruits.
Environmental monitoring and assessment,188(5): 1-11.
31. Thorat L, Oulkar DP, Banerjee K, Nath BB. 2016. Desiccation stress induces
developmental heterochrony in Drosophila melanogaster. Journal of Biosciences41(3):
331-339.
2017-18
1. Adsule PG, Deshmukh MB, Patil CT, Savalekar KR, Shabeer ATP, and Banerjee K. 2017.
Effect of wine industry waste on pesticide degradation in soil. RJLBPCS. 2(6): 33-43.
2. Anupa T and Samarth RR. 2017. Distant hybridization in grapes through embryo rescue
for desirable traits- A review. Research in Environment and Life Sciences. 10(10): 797-
806. (NAAS: 3.74).
3. Garg R, Saha S, Roy BK and Ghule S. 2017. Polyphasic approach for identification and
characterization of Colletotrichum capsici (Syd.) Butler and Bisby causing Anthracnose
disease of Chilli in India. Journal of Mycopathological Research, 55(1):37-50. (NAAS:
4.90).
4. Ghule MR, and Sawant IS. 2017. Potential of Fusarium spp. for biocontrol of downy
mildew of grapes. Pest Management in Hort. Ecosystems, 23(2): 147-152 (NAAS: 4.49).
5. Ghule MR, Sawant IS, and Sawant SD. 2018. Eco-friendly methods for management of
downy mildew of grapevines. Journal of Eco-friendly Agriculture, 13(1): 80- 84. (NAAS:
3.80).
6. Jadhav M, Shabeer ATP, Nakade M, Gadgil M, Oulkar D, Arimboor R, Menon R, and
Banerjee K. 2017. Multiresidue method for targeted screening of pesticide residues in spice
cardamom (Elettaria cardamomum) by liquid chromatography with tandem mass
spectrometry. Journal of AOAC International. 100 (3): 1-7. (NAAS: 6.92).
7. Kamble AK, Sawant SD, Saha S, and Sawant IS. 2017. Screening of grapevine germplasm
to identify sources of resistance to bacterial leaf spot caused by Xanthomonas campestris
pv. viticola. International Journal of Agriculture Innovations and Research. 5: 834-837
(ISSN 2319-1473). (NAAS: 3.99).
8. Kamble AK, Sawant SD, Saha S, Sawant IS. 2017. In vitro efficacy of different chemicals
and biological agents against Xanthomonas campestris pv. viticola causing bacterial leaf
spot of grapes. International Journal of Agriculture Sciences. ISSN: 0975-3710&E-ISSN:
0975- 9107, 9(30): 4427-4430. (NAAS: 4.20).
9. Oulkar DP, Hingmire S, Goon A, Jadhav M, Ugare B, Shabeer ATP, and Banerjee K. 2017.
Optimization and validation of a residue analysis method for glyphosate, glufosinate, and
their metabolites in plant matrixes by liquid chromatography with tandem mass
spectrometry. Journal of AOAC International. 100 (3). (NAAS: 6.92).
10. Ramteke SD, Parhe SD, Deshmukh UV, Urkude V, and Bhagwat SR. 2017. Impact of leaf
thickness on biochemical, yield and quality parameters of grape genotypes. Trends in
Biosciences. 10(17):3028-3033. (NAAS: 3.94).
11. Ramteke SD, Urkude V, Bhagwat SR, Deshmukh UV, and Birhade AP. 2018. A study on
impact of silixol (OSA) on berry cracking in Fantasy Seedless grapes. International Journal
of Agriculture Innovations and Research. 6(4):45-48. (NAAS: 3.99).
12. Ramteke SD, Urkude V, Bhagwat SR, Deshmukh UV, and Birhade AP. 2018. Impact of
water soluble GA tablets in Manik Chaman grapes. International Journal of Agriculture
Sciences. 10(2): 5053-5055. (NAAS: 4.20).
13. Samarth RR, Gaikwad SM, Deore P, Anupa T and Bhosale P. 2016. Profilling of grape
varieties using OIV descriptors and molecular markers. The Biosacan 11 (4):3189-3195.
(NAAS: 5.26).

14. Sankar V & M. Chavan K. Thangasamy A, Gorrepati K, Shabeer ATP, Savalekar KR, and
Banerjee K. 2018. Comparison of organic and conventional farming for onion yield,
biochemical quality, soil organic carbon, and microbial population. Archives of Agronomy
and Soil Scienc. 6792: 219-230. (NAAS: 8.14).
15. Sawant IS, Wadkar PN, Ghule SB, Rajguru YR, Salunkhe VP, and Sawant, SD. 2017.
Enhanced biological control of powdery mildew in vineyards by integrating a strain of
Trichoderma afroharzianum with sulphur. Biological Control. 114: 133-143. (NAAS 8.01,
IF 2.307)
16. Sawant SD, Ghule MR, Sawardekar RS, Sawant IS and Sujoy S. 2017. Effective use of
activated potassium salt of long chain phosphorous (96%) for the control of fungicide
resistant Plasmopara viticola causing downy mildew in grapes. Indian Phytopath.
69(4s):338-344. (NAAS: 5.90).
17. Sawant SD, Savardekar RM, Ghule MR, Sawant IS and Saha S. 2017. Evaluation of
amisulbrom 20% SC against Plasmopara viticola of grapes under in vitro and in vivo
conditions. Indian Phytopath. 69(4s): 621-624. (NAAS: 5.90).
18. Shabeer ATP, Girame R, Utture S, Oulkar D, Pudale A, Banerjee K, Ajay D, Ranjith A,
and Menon KRK. 2018. Optimization of multiresidue method for targeted screening and
quantitation of 243 pesticides residues in spice cardamom (Elettaria cardamomum) by gas
chromatography tandem mass spectrometry (GC-MS/MS) analysis. Chemosphere. 193:
447-453. (NAAS: 10.21).
19. Shabeer ATP, Jadhav M, Girame R, Hingmire S, Bhongale A, Pudale A, Banerjee K. 2017.
Targeted screening and safety evaluation of 276 agrochemical residues in raisins using
buffered e t h y l a c e t a t e e x t r a c t i o n a n d l i q u i d chromatography–tandem mass
spectrometry analysis. Chemosphere 184, 1036-1042. (NAAS: 9.70).
20. Sharma AK, Dagadkhair RA and Somkuwar RG. 2018. Evaluation of grape pomace and
quality of enriched cookies after standardizing baking conditions. Journal of AgriSearch,
5(1): 50-55. https://doi.org/10.21921/jas.v5i01.11134. (NAAS: 4.41).
21. Sharma AK, Dudhane A, Shabeer ATP and Kadam P. 2018. Media Optimization for
primary screening of β-Glucosidase producing yeast strains. Chemical Science Review and
Letters, 7(25): 56-61. (NAAS: 5.21).
22. Sharma AK, Sawant IS, Sawant SD, Saha S, Kadam P and Somkuwar RG. 2017 Aqueous
chlorine dioxide for the management of powdery mildew vis-a-vis maintaining quality of
grapes and raisins. J. of Eco-friendly Agriculture. 12(2): 59-64. (NAAS: 3.80).
23. Sharma AK, Shabeer ATP, Hingmire S, Somkuwar RG, Naik S and Kadam P. 2017.
Enhanced shelf life of Thompson Seedless grapes by application of chitosan. Progressive
Horticulture. 49 (1): 8-13. (NAAS: 3.53).
24. Sharma AK, Somkuwar RG, Bhange MA and Samarth RR. 2017. Evaluation of grape
varieties for juice quality under tropical conditions of Pune region. Proceedings of the
National Academy of Sciences, India Section B: Biological Sciences. DOI
10.1007/s40011-017-0894-4. (NAAS: 5.00)
25. Upadhyay A, Maske S, Satisha J, Kadoo N, Gupta VS. 2018. GA application in grapes
(Vitis vinifera L.) modulates different sets of genes at cluster emergence, full bloom and
berry stage as revealed by RNA sequence based transcriptome analysis. Functional and
Integrative Genomics, DOI: 10.1007/s10142-018-0605-0. (IF – 3.496). (NAAS: 8.27).


Annexure 4
Details of Intellectual Property Rights
Patents
Title of Work Date of Application Status of
Filling number Application
A method for extraction of anthocyanins and 22/06/2017 201711021975 Filed
composition thereof
A method for preparation of Enriched yoghurt 14/06/2017 201711020822 Filed
Copyrights
Copyright title Application Date of grant
No/Diary No:
A web based online grapevine disease and pest 9478/2016-CO/SW 31/01/2018
diagnosis system for India
Diagnostics and management of pests of table 9479/2016-CO/SW 31/01/2018
grapes, a ready reckoner for diagnosis and
management of grapevine pests in India
Grapevine diseases in India a ready reckoner for 9480/2016-CO/SW 31/01/2018
diagnosis and management of grapevine diseases in
India
Catalogue of Indian grape Vitis spp. germplasm 9481/2016-CO/L 31/01/2018
volume I
Information system for management of SW-8145/2014 13/10/2014
microsatellite data for grape Germplasm in India
Trademarks
Trademark for institute logo ( ) was obtained for the following trademark classes
Trademark classes and description of class Certificate Date Of
Number Grant
Class 33: Alcoholic beverages(except beers) 1549205 11/05/17
Class 42: Scientific and technological services and research and 1547118 08/05/17
design relating thereto; industrial analysis and research services;
design and development of computer hardware and software.
Class 31: Agricultural, horticultural and forestry products and 1549037 11/05/17
grains not included in other classes; live animals; fresh fruits and
vegetables; seeds, natural plants and flowers; foodstuffs for
animals, malt
Class 32:Beers, mineral and aerated waters, and other non-alcoholic 1548515 11/05/17
drinks; fruit drinks and fruit juices; syrups and other preparations
for making beverages

Trademark classes and description of class Certificate Date Of
Number Grant
Class 44: Medical services, veterinary services, hygienic and beauty 1549779 12/05/17
care for human beings or animals; agriculture, horticulture and
forestry services.
Technologies commercialized
Name of the Name of Licensee/ Type of Mode of Date of
Technology Contracting Party Partnership Tech. Transfer
(Technology Transfer
Licensing/ (MoU/MoA/
Consultancy License/
/ Contract MTA etc.)
Research)
Developing multi residue Spices Board, Cochin Contract MoU 20/11/2014
method for analysis of Research
pesticide residues in
three spice matrix viz.,
cardamom, chilli and
cumin
Weather based grape Deepsagar Agro Technology MoA 22/11/2014
advisory Enviro Services Pvt Licensing
Ltd.
Weather based grape Horticulture Technology MoA 03/05/2015
advisory Department of Licensing
Karnataka State
Commercialization of Zytex Biotech Pvt. Technology MoU 22/11/2016
Bacillus Strain DR 39 for Ltd. Mumbai Licensing
pesticide degradation and
plant growth promotion


Annexure 5
Awards and recognitions
Accreditation
 The Centre has ISO 9001:2015 Certificate No. GCS/QMS/2533.
 National Referral Laboratory of the Centre has been accredited by NABL for chemical
testing for pesticide residues (207 Nos.) in fruits and vegetables and aflatoxins (B1, B2,
G1, G2) in nuts and cereals. Accreditation Certificate No. TC-7975 (Chemical Testing)
valid till 09/10/2020.
 National Referral Laboratory of the Centre has been accredited by NABL for
proficiency testing provider for pesticide residues (207 Nos.) in fruits and vegetables
and aflatoxins (B1, B2, G1, G2) in nuts and cereals. Accreditation Certificate No.
PC1027 valid till 05/06/2020.
Awards
 Dr S.D. Sawant and Dr. K. Banerjee were honoured with Crystal National Agri-Award,
2014, constituted by Krishi Anusandhan and Kisan Vikas Foundation, New Delhi. Dr
K. Banerjee received the award under scientist category whereas Dr S.D. Sawant
received special jury award.
 Dr. S.D. Sawant received ‘Shri S.A. Dabholkar Prayog Pariwar Puraskar 2016’.
 Dr. S.D. Sawant received “9 State Level Agro Care Idol” Award by Agrocare Krushi
Manch at Pimpalgaon, district Nashik on 6th October 2017.
 Dr. Indu S. Sawant received “Best Woman Scientist Award” during “International
Conference: Biodiversity, Bioresources and Biotechnology” organized by Association
for the Advancement of Biodiversity Science; Society for Applied Biotechnology;
Imperial Scientific Publishing during 30-31st January 2014.
 Dr. Indu S Sawant was conferred with the ‘Sharda Lele Memorial Award’ by the Indian
Phytopathological Society, New Delhi. The award lecture on ‘Tapping the marvellous
power of microorganisms for sustainable agriculture’ was delivered during the 69th
Annual Meeting and the National Symposium on “Diagnosis and Management of Plant
Diseases: Integrated Approaches and Recent Trends” held at ICAR Research Complex
for NEH region, Umiam, Meghalaya during 9-11th January 2017.
 Dr. K. Banerjee received Harvey W. Wiley award of the AOAC International- 2017.
 Dr. S.D. Ramteke received Outstanding Scientist Award 2017 by The society of
Tropical Agriculture, New Delhi.
 Dr. A.K. Sharma received first award for his slogan in a competition organized by
Rajbhasha Section of ICAR in a special function of ICAR at New Delhi on 1st Jan 2014.
Fellows of societies
 Dr. Indu S. Sawant: Fellow of the Association for the Advancement of Biodiversity
Science, Karnataka
 Dr. Indu S Sawant: Fellow of Phytopathological Society of India, New Delhi.
 Dr. Indu S Sawant: Fellow of the Society for Biocontrol Advancement, Bengaluru.
 Dr. R.G. Somkuwar: Fellow of Horticulture Society of India, New Delhi.

 Dr. K. Banerjee was elected as the President, AOAC, India Section
 Dr. K. Banerjee: Fellow of the National Academy of Agricultural Sciences, New Delhi
Members in Professional Societies
 Dr. K. Banerjee was nominated as Member- Panel for Method of Sampling and
Analysis, Food Safety Standards Authority of India
(http://www.fssai.gov.in/MediaCenter/ScientificPanels.aspx).
Editorial boards
 Dr. Indu S. Sawant: Member in National Editorial Board of The Horticultural Society
of India.
 Dr. K. Banerjee: Member- Editorial Board of Chemical and Biological Technologies in
Agriculture (Springer Plus: http://www.chembioagro.com/about/edboard).
 Dr. K. Banerjee: Member- Editorial Board of the journal of Plant Protection Sciences
(The Official Publication of the Association for Advancement in Plant Protection,
Regn. No. S/1L37974/2006-07) (http://www.aappbckv.org/ journal/editorialboard.php)
 Dr. A.K. Sharma: Associate Editor of research Journal ‘Progressive Horticulture’.
 Dr. Roshni Samarth nominated as associate editor of research Journal “International
Journal of Agricultural Sciences”.
Members of Committees
International
 Dr. K. Banerjee was selected as Member- Program Advisory Committee (PAC),
Malaysian Palm Oil Board (MPOB) in the subject area of food nutrition and quality.
The main role of this PAC is to review the research programs of MPOB and assess the
progress of their research projects purely on advisory capacity.
National
 Dr. S. D. Sawant was nominated as DG's nominee to Board of Management of UHS,
Bagalkot and Executive Council of MPKV, Rahuri.
 Dr. S.D. Sawant was the member of the following committees
 Departmental Promotion Committee at ICAR-NIASM, Baramati
 Assessment committee for assessment of Technical Officer for promotion at ICAR-
NIASM.
 Interview Committee to process promotion proposals of Associate Professors to
Professors of MPKV, Rahuri.
 Selection Board for finalizing promotion proposals under CAS for Professors of
VNMKV, Parbhani.
 Screening cum Evaluation/ Interview committee of Academic Staff of MPKV
Rahuri.
 MCAR committee for promotions under CAS at PDKV, Akola.
 Interview Panel for selection of vacant posts at KVK, Bhabhaleshwar, Dist.
Ahmednagar

 Selection Board for promotion of Associate and Assistant Professor at MPKV,
Rahuri.
 Selection Committee meeting convened by ICAR-DFR, Pune as nominee of the
DDG (HS), ICAR
 Dr. Indu S. Sawant was member of the following committees
 Departmental Promotion Committee as DDG’s nominee at ICAR-DMARP,
Anand.
 DG’s nominee as subject expert for CAS- DPC of Scientist Plant Pathology
(DOG, Rajgurunagar).
 Adjunct Professor in subject of Botany by Vasant Dada Sugar Institute, Pune.
 Dr. R.G. Somkuwar was the member of the following committees
 Screening cum evaluation committee for considering the applications of academic
staff member of MPKV, Rahuri.
 Institute Management Committee (IMC) of ICAR-DFR, Pune.
 IMC of ICAR-NRCP, Solapur.
 Departmental promotion committee for the promotion of technical staff
(Horticulture) at ICAR-NIASM, Baramati
 Dr. Anuradha Upadhyay was the member of the following committees
 Member of DBT Accreditation Panel of National Certification System for Tissue
Culture Raised Plants (NCS-TCP).
 Institute Management Committee (IMC) of ICAR-NRCG, Pune for a period of 3
years.
 Institute Management Committee (IMC) of ICAR-NBAIR, Bengaluru for a period
of three years w.e.f January 2018.
 DBT nominee of Institute Biosafety committee of ICAR-DOGR, Pune for a period
of three years.
 Selection Committee as subject matter expert for the selection of Assistant
professor (Biotechnology) at VNKV, Parbhani
 Selection Committee as a subject matter expert for the selection of Associate
professor (Biotechnology) and Associate professor (Molecular
Biology/Biochemistry) at VNKV, Parbhani.
 As external expert in Institute Biosafety committee (IBSC) of MPKV Rahuri
 Committee for Probation Clearance of Senior Scientist as the subject matter
specialist at ICAR-DFR, Pune on 15th January 2018.
 Dr. A.K. Upadhyay was nominated by ICAR as Vigilance Officer at the Centre.
 Dr. A.K. Upadhyay was the member of the following committees
 Institute Management Committee of ICAR-NRCG, Pune for the period of three
years from 2013-14.
 DPC for the assessment of technical staff (Farm management) at ICAR-NIASM.

 IMC of ICAR-National Institute of Abiotic Stress Management, Baramati.
 Scientific Advisory Committee of KVK, Narayangaon.
 Dr. Kaushik Banerjee, Dr. Ahammed Shabeer T.P. and several other staff of NRL were
nominated as the Chairman and members of Electronic Working Groups set up by the
Food Safety Standards Authority of India (FSSAI) for drafting the Indian standards for
harmonization with the International standards. They prepared the draft standards for
several agricultural commodities and submitted to FSSAI.
 Dr. K. Banerjee was nominated for the following committees
 Review Committee constituted by the Director General- Indian Council of Medical
Research to review the progress of the project on Nutritive Value of Indian Foods
currently ongoing at the National Institute of Nutrition, Hyderabad. The review was
carried out on 8th March 2014.
 Research Advisory Committee of ICAR-IIVR,Varanasi (2014-2017)
 FSSAI Scientific Panel on ‘Pesticides and Antibiotic Residues’ and ‘Methods of
Sampling and Analysis’.
 Dr. S.D. Ramteke was nominated for the following committees
 Department Promotion Committee to clear probation of 4 scientists at National
Institute on Abiotic Stress Management, Baramati.
 Selection Committee of Technical personnel of ICAR-National Institute of Abiotic
Stress Management, Baramati and Directorate of Onion and Garlic Research,
Rajgurunagar, Pune.
 Departmental Promotion Committee to clear probation of scientists of ICAR-
NIASM, Baramati
 Dr. J. Satisha were nominated by ICAR as member of Institute Management Committee
of NRC Grapes, Pune for the period of three years from 2013-14.
 Dr. A.K. Sharma was the Chairman of Poster Evaluation Committee for two thematic
areas and Co-chairman for another 3 areas in the International Symposium on
Innovations in horticulture for nutritional security, conserving biodiversity and poverty
alleviation during 16-18th October 2014 at Babasaheb Bhimrao Ambedkar University,
Lucknow.
 Mrs. Kavita Y. Mundankar was the member of DPC for the promotion of technical staff
(Computer Science) at ICAR-NIASM, Baramati.
 Dr. Ahammed Shabeer T.P. was a member of Techno Scrutiny Committee for the
scheme for setting up/ upgradation of food testing laboratories under Ministry of Food
Processing Industries.
 Ms. Anupa T. was a member of team of NHB for rating and accreditation of nurseries
in Maharashtra.
 Mr. S.N. Salve, Administrative Officer attended recruitment committee meeting as
ICAR representative at ICAR Research Complex for Goa, Goa during 6-7th and 24-
26th August 2014.

Examiners / Reviewers
 Dr. S.D. Sawant, Dr. Indu S. Sawant and Dr. R.G. Somkuwar attended Scrutiny
Committee meeting for Ph.D. thesis in the subject of Agrochemicals and Pest
Management at Shivaji University, Kolhapur on 11th July 2017.
 Dr. S.D. Sawant attended meeting to conduct viva-voce examination of Ph.D. student
at MPKV, Rahuri.
 Dr. Indu S Sawant examined the Ph. D. thesis of Sujeewa Rathnayake, titled:
‘Characterization of Aureobasidium pullulans (De Bary) on Vitis vinefera L. (cv
Chardonnay) with biocontrol of bitter rot, Greeneria uvicola’ submitted to Charles Strut
University, Australia.
 Dr Indu S. Sawant acted as reviewer for Indian Phytopathology, Indian Journal of
Horticulture, BSPP-Plant Pathology, Chemosphere and Desalination and Water
Treatment.
 Dr. Indu S Sawant reviewed a project proposal submitted to The Natural Sciences and
Engineering Research Council of Canada (NSERC).
 Dr. R.G. Somkuwar and Dr. J. Satisha were invited as external examiner for qualifying
viva - voce of Ph. D (Pomology) student at MPKV, Rahuri on 11th September 2013.
 Dr. R.G. Somkuwar was a member of evaluation committee for Ph. D. thesis viva of
MPKV, Rahuri.
 Dr. Anuradha Upadhyay recognized as a reviewer by Biologia Plantarum (Springer
publication), Journal of Ecosystem (Hindawi publication), Journal of Horticultural
Sciences (SPH publication), SciTechnol and Applied Biochemistry and Biotechnolgy
(springer), Journal Plant and Soil.
 Dr. Anuradha Upadhyay was recognized as Ph.D. examiner by Shivaji University and
reviewed a Ph.D. thesis entitled “Identification and molecular analysis of the genes
associated with sucrose accumulation in sugarcane” by Shri S.B. Kalwade.
 Dr. Anuradha Upadhyay was recognized as Ph.D examiner by Shivaji University,
Kolhapur and M.Sc (Ag. Biotechnology) examiner by UAS Dharwad.
 Dr. Anuradha Upadhyay was recognized by Science and Engineering Board (SERB) as
project referee.
 Dr. A.K. Upadhyay was nominated as Controller of Examinations for conducting
exams for recruitment of LDC, Stenographer and AFAO at the Centre.
 Dr. S. D. Ramteke was member of selection committee at DOGR, Rajgurunagar for
recruitment of T1 (farm) and lab technicians.
 Dr. S.D. Ramteke conducted qualifying examination of M. Sc. (Agriculture) students
of UAS Raichur on 19th December 2014.
 Dr. S.D. Ramteke attended Ph.D. thesis viva as an external examiner at Dr. PDKV,
Akola during 27-29th December 2017.
 Dr. A.K. Sharma was recognized as referee to evaluate Ph. D. thesis of a student of
Homi Bhabha National Institute, Mumbai.
 Dr. A.K. Sharma was recognized as expert for scrutiny of Ph.D. synopsis in Department
of Food Science of Shivaji University, Kolhapur.

 Dr. A.K. Sharma evaluated Ph.D. thesis of Department of Horticulture, Dr. PDKV,
Akola and conducted viva voce examination of Ph.D. students.
 Dr. A.K. Sharma evaluated Ph. D/M.Sc. (Horticulture) thesis and conducted viva-voce
examination of K.R.C. College of Horticulture, Arabhavi, PG Centre of UHS,
Bengaluru on 14th March 2017, Bihar Agricultural University, Sabour (Bhagalpur)
 Dr. A.K. Sharma reviewed the research articles for the journals Indian Journal of
Horticulture, Indian Journal of Experimental Biology, Progressive Horticulture etc.
 Dr. A. K. Sharma and Mrs. Kavita Y. Mundankar was nominated as outside expert
member of the Departmental Promotion Committee constituted to assess the Technical
staff (Category–III) at ICAR-DOGR, Rajgurnagar Pune in “Functional group of
Laboratory Technical (Computer)”.
 Dr. Roshni R. Samarth enacted as reviewer for International of Agri. J. Sciences and
Indian Journal of Horticulture.
 Dr. Ahammed Shabeer T. P. was nominated as member of recruitment committee for
filling of the post of lower division clerk at NRC for Pomegranate, Solapur.
Ph.D. Guide
 Shivaji University, Kolhapur has recognized following scientists as research guide in
different subjects mentioned below:
 Agrochemicals and Pest Management
 Dr. S.D. Sawant
 Dr. Indu S. Sawant
 Dr. R.G. Somkuwar
 Dr. A.K. Upadhyay
 Dr. K. Banerjee
 Dr. S.D. Ramteke
 Dr. D.S. Yadav
 Microbiology
 Biotechnology
 Dr. Anuradha Upadhyay
 Environmental Science
 Dr. K. Banerjee
 Botany
 Dr. S.D. Ramteke
 Food Science and Technology
 Dr. A.K. Sharma
 Shri Jagdishprasad Jhabarmal Tibrewala University, Rajasthan has recognized Dr. Indu S.
Sawant as research guide for Ph.D. in Botany.

 University of Horticultural Sciences, Bagalkot has recognized following scientists as PG
Teacher in different subjects mentioned below:
 Plant Pathology
 Horticulture (Fruit Science)
 Dr. R.G. Somkuwar
 Dr. A.K. Sharma
 Molecular Biology and Biotechnology
 Dr. Anuradha Upadhyay
 Plant Bacteriology
 Dr. Sujoy Saha
 Agricultural Chemistry
 Dr. Ahammed Shabeer T.P.
 Bharati Vidyapeeth, Pune has recognized Dr. Ahammed Shabeer T.P. as a Ph.D. guide in
the subject of Biochemistry with effect from 23/03/2017 for a period of 5 years.
 Mahatma Phule Krishi Vidyapeeth, Rahuri has recognized Dr. R.G. Somkuwar as
recognized teacher for M.Sc. and Ph.D. (Horticulture).
Awards for posters / papers
 Poster presentation “Degradation Kinetics of Forchlorfenuron, 6-Benzyl adenine,
Gibberellic acid (GA3) and Ethephon in Grapes (Vitis vinifera)” by Ugare et al received
best poster award in National Conference of Plant Physiology – 2013 organized at
Directorate of Groundnut Research, Junagadh held on 13-16th December, 2013.
 Poster presentations “Development of analytical method for analysis of volatile
compounds from Indian wines using gas chromatography mass spectrometry coupled
with thermal desorption system” by Kamble, N. et al. and “Degradation kinetics of
Buprofezin, Imidacloprid and Flubendiamide residues in Okra (Abelmoschus
esculentus L. (Moench)” by Hingmire, S. et al. received 1st and 2nd prize respectively
in National Seminar on “New Trends in Pest Management” organized at Shivaji
University, Kolhapur on 31st January, 2014.
 A paper entitled “Institutional Role on Promotion of Good Agricultural Practices
(GAP) and Export of Grapes in Maharashtra” authored by Sukanya Som, R. Roy
Burman, V. Sangeetha, V. Lenin, J. P. Sharma, K. Banerjee and Indu Sawant was
conferred with Best Paper Award-2015 in 7th National Seminar on “Sustainable Rural
Livelihood: Technological and Institutional Perspective” organized by Society for
Community Mobilization for Sustainable Development at Sher-E—Kashmir University
of Agriculture & Technology for Jammu, Jammu during 8 10th January, 2015.
 A poster entitled ‘Rootstock regulates fruit and wine quality parameters of Cabernet
Sauvignon grapevine (Vitis vinifera L.)’ authored by Satisha J., Kitture A. R., Sharma
K., Sharma J. and Somkuwar R.G. and presented in “6th Indian Horticulture Congress”
organized by The Horticultural Society of India, New Delhi in collaboration with
TNAU, Coimbatore, Tamil Nadu on 6th to 9th November, 2014 received Best Poster
Presentation award in the technical session on“ Production technologies”.

 Oral presentation “Seasonal incidence, host and host stage preference of mealy bug
parasitoid, Anagyrus dactylopii Howard” by Amala U. was awarded 2nd Prize during
International Conference on ‘Changing scenario of pest problems in agri – horti
ecosystem and their management’ during 27-29th November 2014 and organised by the
Entomological Research Association at Department of Entomology, Maharana Pratap
University of Agriculture and Technology (MPUAT), Udaipur.
 The poster presentation “Sawant SD, Ghule MR, Savardekar RM, Sawant IS, Saha S:
Effective use of potassium salts of phosphorus (96%) in the control of fungicide
resistant Plasmopara viticola causing downy mildew in grapes” was awarded the first
prize for poster presentation in 6th International Conference on “Plant, Pathogens and
People” organized by the Indian Phytopathological Society, New Delhi during 23rd -
26th February 2016 at NAAS Complex, New Delhi.
 Oral presentation “An integrated approach of molecular breeding for downy and
powdery mildew resistance in grape” by Roshni R. Samarth, Pushpa Deore, Anuradha
Upadhyay and Indu Sawant received second prize in the National Conference on Fruit
Breeding in Tropics and Subtropics-An Indian Perspective at ICAR-IIHR Bengaluru
from 27-29th April 2016.
 Dr. S.D. Ramteke and team has received the first prize for research paper presentation
in National Symposium on Applications of Radioisotopes and Tracer Techniques in
Agriculture and Environment, held at TNAU, Coimbatore during 16-17th February
2017.
 Poster entitled “Evaluation of grape rootstocks for yield and quality parameters of
Sauvignon Blanc wine grapes” by R.G. Somkuwar; A.K. Sharma and Mahadev Bhange
was awarded as Best Poster in Session V in the 7th Indian Horticulture Congress 2016
organized by Horticulture Society of India, at IARI, New Delhi, 15-18 November 2016.
 Posters entitled “Extraction of polyphenols from grape seeds: towards a green eco-
friendly analytical technique” and “Development of a non-targeted screening method
for the analysis of pesticides in various spices using ultrahigh performance liquid
chromatographyhigh resolution (orbitrap) mass spectrometry”presented by Dr.
Ahammed Shabeer T.P. in 4th Annual Conference of the India Section of AOAC
International, New Delhi received Best Poster Awards Sponsored by Royal Society of
Chemistry.
Invited speakers
 Dr. S.D. Sawant was invited to deliver a talk on “Strategies for safe and judicious use
of pesticides in grape” in seminar on “Safe and judicious use of agrochemicals and
application of green chemistry” organized by FICCI on 10th March, 2014 at Pune.
 Dr S.D. Sawant was invited to deliver a lecture on ‘Weather Data based disease
management in Horticultural crops’ in the National Symposium on “Agrochemicals for
Food and Environment Safety” organized by Society of Pesticide Science India at IARI,
New Delhi on 28.01.2015.
 Dr S.D. Sawant delivered an invited lecture on the occasion of Dr. P.B. Chavan Lecture
Series at Dept. of Botany, Y.C. Institute of Science, Satara on February 18, 2015.
 Dr. Indu S. Sawant was invited to deliver a talk on “Trichoderma - Biotechnology and
strategies for biocontrol of foliar fungal pathogens” in International Conference on
Advances in Biotechnology and Bioinformatics 2013’ at Pune during 25-27th
November, 2013.

 Dr. Indu S. Sawant was invited to deliver a talk on “Utilization of the functional
diversity of plant associated microbes for biological control of fungal diseases of
horticultural crops” in the International Conference Biodiversity, Bioresources and
Biotechnology, Mysore during 30th – 31st January, 2014.
 Dr. Indu S. Sawant was invited to deliver a talk on “Biological control of Phytopthora
using Trichoderma spp.” in the “National Citrus Meet” organized by PPV&FRA and
NRC for Citrus on 13th August, 2013 at Nagpur.
 Dr. Indu S. Sawant was invited to give a lead talk on ‘Molecular characterization of
mating type idiomorphs of Erysiphe necator from India’ in the National Symposium
on ‘Understanding Host-pathogen Interactions Through Science of Omics’ organized
by the Indian Phytopathological Society, New Delhi at IISR, Calicut on 16-17th March
2015.
 Dr. Indu S. Sawant was invited to give a lead talk on ‘Application of selected superior
strains of microorganisms to minimize risks associated with fungicide based disease
management in grapes’ in the International Symposium on ‘Innovations in Horticulture
for Nutritional Security, Conserving Biodiversity and Poverty Alleviation’ during 16-
18th October organized by Department of Applied Plant Science, Babasaheb Bhimrao
Ambedkar University, Lucknow and Indian Society of Horticultural Research and
Development.
 Dr. Anuradha Upadhyay was invited as expert speaker during national seminar on
“Accelerating Biology 2014: Computing Life” held during February 18-20, 2014 at
Pune and organized by cDAC Pune. She delivered talk on “RNA seq based analysis of
salinity stress response in grapevine (Vitis vinifera L.)“.
 Dr. Anuradha Upadhyay was invited as expert speaker during International Conference
on “NextGen genomics and bioinformatics tools” held at National Institute of Mental
Health and Neuro Sciences, Bengaluru during 17-19th November 2014.
 Dr. K. Banerjee was invited to deliver a lecture “Challenges in pesticide residue
analysis in India” in Waters Food Summit at ITC Grand Chola, Chennai during 27-28th
May 2013.
 Dr. K. Banerjee was invited to deliver a lecture “Screening of fruits and vegetables for
the residues of pesticides and other emerging food contaminants by GC-MS and LC-
MS” in Analytica Anacon. Session: "Food Safety and Standards - Priorities for India"
at Mumbai on 13th November, 2013.
 Dr. K. Banerjee was invited to deliver a lecture “Application of molecular imprinting
technology in contaminant residue analysis” in Indo-UK Seminar “Molecular
Imprinting: Strategies, Applications & Future Perspective” organized by National
Environmental Engineering Research Institute (NEERI), Nagpur, during 5-7th
February, 2014.
 Dr. K. Banerjee was invited to lecture on ‘Multiresidue analysis of pesticides utilizing
advanced analytical tools’ in the National Symposium on Agrochemicals for Food and
Environmental Safety at IARI, New Delhi during 28-30th January 2015.
 Dr. K. Banerjee delivered an invited lecture on ‘Overview of food safety programs in
India: residue monitoring taking advantage of advanced analytical tools’ in the
‘Symposium on the Application of Chromatography and Spectroscopy Techniques in
Pharma and Food Analysis’ organized by Chromatographic Society of India, Mumbai
during 18-19th December 2014.

 Dr. K. Banerjee delivered an invited lecture on ‘Pesticide residues in pomegranate’ in
the National Seminar-Cum-Exhibition on Pomegranate for Nutrition, livelihood
Security and Entrepreneurship Development during 5-7th December 2014.
 Dr. K. Banerjee delivered an invited lecture on ‘Heavy metals and pesticide residues in
horticultural crops’ in the Indian Horticulture Congress, Coimbatore during 6-9th
November 2014.
 Dr. K. Banerjee delivered an invited lecture on ‘Targeted and non-target screening and
quantification of contaminant residues in agricultural commodities: Taking advantage
of advanced analytical tools’ in the 13th IUPAC International Conference Symposium
on effective residue analysis utilizing advanced analytical tools during 10-15th August
2014.
 Dr. K. Banerjee delivered an invited lecture on ‘Pre-harvest contaminant management
for better quality vegetables’ in the National Conference on ‘Pre-/Post-Harvest Losses
& Value Addition in Vegetables’ during 12-13th July 2014 at Indian Institute of
Vegetable Research, Varanasi.
 Dr. K. Banerjee delivered an invited lecture on ‘Some novel visions and resolutions in
adopting frontier analytical tools and developing methodologies: a saga of building a
community-friendly food safety system’ in the National Academy of Agricultural
Sciences, New Delhi on 4th June 2014.
 Dr. S.D. Ramteke gave a lead presentation ‘Physiology of Flowering of grapevine’ at
Lucknow on 24-26th May 2014.
 Dr. A.K. Sharma was invited to give lecture on “Value addition in grapes” in the
International Symposium on Innovations in horticulture for nutritional security,
conserving biodiversity and poverty alleviation during 16-18th October, 2014 at
Babasaheb Bhimrao Ambedkar University, Lucknow.
 Invited presentation by Dr. A. K. Sharma on “Present status and future prospects of
grape production in hills with special reference to Uttarakhand” was awarded first prize
in the seminar on 'Emerging trends in high-tech horticulture under changing climate'.
This seminar was organized by ICAR-Central Institute of Temperate Horticulture,
Regional Station, Mukteshwar during 6-7th March 2018.
 Invited Lecture by Dr. D.S. Yadav on 'Potential of pest risk forecasting and automated
advisory for high-tech hill-horticulture' was awarded First position for Crop Protection
Session in Seminar on “Emerging trends in Hi tech Hill Horticulture under changing
climate” during March 6-7, 2018 at ICAR-CITH (RS), Mukteshwar, Uttarakhand.
 Dr. Ahammed Shabeer T. P. was invited to deliver a lecture “Organic contaminants in
fruits and vegetables” in National Seminar on “Soil Fertility, Degradation and
Contaminants” organized by Dapoli Chapter of Indian Society of Soil Sciences,
DBSKKV, Dapoli during 8-9th May 2013.


Annexure 6
Details of inter-institutional collaborations
Collaborting institute Area of collaboration
Department of Horticulture, Popularizing advisory systems for grape growers in
Government of Karnataka North Karnataka
Agharkar Research Institute, Pune Collaboration for breeding for downy and powdery
mildew and semiochemical studies
APT Research Foundation, Pune Validate the immune-affinity column for aflatoxin
analysis.
Bhabha Atomic Research Centre, Collaborated with BARC for radio isotopic studies
Mumbai
ICAR-Central Tobacco Research Developing analytical protocols for pesticide
Institute, Rajahmundry residues in tobacco.
ICAR-Directorate of Floricultural Detection and identification of virus and
Research, Pune phytoplasma in crops
ICAR-Indian Agricultural Research Development of grape growth model, Identification
Institute,National Agricultural Science of insects
Fund
ICAR-Indian Institute of Horticultural Semio chemical studies;
Research, Bengaluru
ICAR-National Bureau of Preserving important microbial cultures
Agriculturally Important
Microorganisms, Mau
ICAR-National Research Centre on Analysis and method development for pesticide
Seed Spices, Ajmer residues in cumin and coriander .
M/s Shivrai Tehnologies Developing DSS software
Mahalanobis National Crop Forecast Use of high resolution remote sensing data for
Centre precision viticulture
National Chemical Laboratory, Pune Transcriptome analysis of salinity and GA3 response
NBAIR, Bengaluru Insect identificaiton
Panjab University, Chandigarh for breeding for powdery mildew
PPVFRA Validation of DUS descriptors and protection of
farmers’ varieties
Spices Board, Cochin Develop analytical protocols for pesticide residues in
spices


Annexure 7
Details of field demonstration of technologies
1. Demonstration of grape variety Manjari Medika at UHS, Bagalkot, Karnataka
2. Demonstration of grape variety Manjari Medika at Theni, Tamil Nadu
3. Demonstration of grape variety Manjari Medika at AICRP (Fruits) – Grapes Centre,
Mandsaur, Madhya Pradesh
4. Demonstration of wine hybrid (Chardonnay × Arkavati) Charak1 at Sula Vineyards,
Nasik, Maharashtra
Nasik, Maharashtra
Nasik, Maharashtra
Nasik, Maharashtra
8. Demonstration of irrigation schedule and technologies for Thompson Seedless on
Dogridge rootstock to increase water use efficiency at MRDBS Farm, Pune
Dogridge rootstock to increase water use efficiency at Walva, district Sangli,
Maharashtra
Dogridge rootstock to increase water use efficiency at Jath, district Sangli, Maharashtra
Dogridge rootstock to increase water use efficiency at Palsi, district Sangli, Maharashtra
Dogridge rootstock to increase water use efficiency at Sawargaon, district Nasik,
Maharashtra
13. Demonstration of Pest management module for ‘zero’ pesticide residues in table grapes
at Niphad, district Nasik, Maharashtra
at Tasgaon, district Sangli, Maharashtra
at Narayangaon, district Pune, Maharashtra
16. Demonstration of biological control agents for disease management at Niphad, district
Nasik, Maharashtra
17. Demonstration of biological control agents for disease management at Tasgaon, district
Sangli, Maharashtra
18. Demonstration of biological control agents for disease managementat Narayangaon,
district Pune, Maharashtra
19. Demonstration of Bacillus subtilis strain (Strain DR-39) for bioremediation of pesticides
at Niphad, district Nasik, Maharashtra
at Tasgaon, district Sangli, Maharashtra
at managementat Narayangaon, district Pune, Maharashtra


Annexure 8
Training acquired by staff
S. Name and Training Title Duration Organized by
No. designation of the
staff
I. Scientific
1. Dr. S.D. Sawant, Executive Development 22-26th February ICAR-NAARM,
Director Programme on Leadership 2014 Hyderabad
Development
2. Dr. Indu S. Sawant, Competency Enhancement 21-23rd February ICAR-NAARM,
Pr. Scientist (Plant Programme for Effective 2017 Hyderabad
Pathology) Implementation of Training
Functions by HRD nodal Officers
of ICAR
3. Dr. Anuradha Genetic transformation methods in 10 days (2017-18) Vasantdada Sugar
Upadhyay, Pr. plants Institute, Pune
Scientist
(Biotechnology)
NGS software tools for crop October 23-26, ICAR-NBPGR, New
genome data analysis 2013 Delhi
4. Dr A K Upadhyay, Training cum Orientation Program 22nd January Lalbagh, Bangalore
Pr. Scientist (Soil on WineNet 2013
Science)
5. Dr. K. Banerjee, Pr. Basic fundamental, maintenance 17th – 20th Shimadzu Analytical
Scientist and software operations for Gas November 2016 (India) Pvt. Ltd. at
(Agricultural Chromatograph-Mass Spectrometer ICAR-NRCG, Pune
Chemistry) (TQ-8040)
6. Dr. S.D. Ramteke, Training Workshop on ‘Stress 12-14th February ICAR-NAARM,
Pr. Scientist (Plant management – medical angle’ 2013 Hyderabad
Physiology)
7. Dr. A.K. Sharma, Refresher course on ‘Agricultural 14-26th July 2014 ICAR-NAARM,
Pr. Scientist Research Management’ Hyderabad
(Horticulture)
Training cum Orientation Program 22nd January Lalbagh, Bangalore
on WineNet 2013
8. Mrs. Kavita Introduction to GIS 5-30th May 2014 National Remote
Mundankar, Sensing Centre
Scientist (NRSC), ISRO,
(Computer Hyderabad
Applications)
Sensitization cum Training 27th February ICAR-IASRI, New
Workshop for Nodal Officer of 2014 Delhi
IPv6
Mobile for Development, a from 2nd October IIT Kanpur and
massive online course 2013 Common wealth of
learning

staff
9. Dr. D.S. Yadav, Electrophysiological recording and 13-17th June 2016 CSIR-NCL, Pune
Scientist analysis of insect chemosensory
(Entomology) responses
10. Dr. Roshni Brainstorm meeting cum Training 21-22nd February Society for
Samarth, Scientist on Cryopreservation and in vitro 2014 Promotion of
(Plant Breeding) conservation of H-PGR Horticulture,
Bengaluru at ICAR-
IIHR, Bengaluru
11. Dr. Ahammed laboratory training programme 27-28thMarch National Institute of
Shabeer T.P. ‘Inter Lab Comparison / 2018 Training for
Proficiency Testing and Standardization
Evaluation of Scores’ (NITS), Noida at
Mumbai
Advance training programme on 14-18th March AB Sciex, Gurgaon
high resolution LC-MS/MS 2017
Advance training programme on 17 – 21st January AB Sciex , Gurgaon
lipidomics 2017
Basic fundamental, maintenance 17th – 20th Shimadzu Analytical
and software operations for Gas November 2016 (India) Pvt. Ltd. at
Chromatograph-Mass Spectrometer ICAR-NRCG, Pune
(TQ-8040)
LC-MS/MS for the identification 8-19th June 2015 At Joint Institute for
of chemical contaminants in food Food Safety and
Applied Nutrition,
University of
Maryland, USA.
12. Dr. Amala U. Detection and measurement of 2-11th September ICAR-NBAII,
Scientist insecticide resistance including 2013 Bangalore
(Entomology) molecular aspects in Insects
13. Ms. Anupa T., ICAR sponsored summer school on 1-21st July 2016 University of
Scientist (Fruit ‘Exploring Genomics Resources Horticultural
Science) for the Improvement of Sciences, Bagalkot,
Horticultural Crops’
14. Ms. Sharmistha Winter School on Recent Advances 12thJanuary – ICAR-Indian
Naik, Scientist in Breeding Approaches and 1stFebruary 2017 Agricultural
(Fruit Science) Varietal Development of Perennial Research Institute,
Horticultural Crops New Delhi
15. Dr. B.B. Fand, Electrophysiological recording and 13-17th June 2016 CSIR-NCL, Pune
Scientist analysis of insect chemosensory
(Entomology) responses
16. Dr. D.N. Gawande, Competency enhancement 15-17thFebruary ICAR-NAARM,
Scientist (Plant programme for effective 2018 Hyderabad
Breeding) implementation of training
functions by HRD Nodal Officers
of ICAR’

staff
Advanced Statistical Techniques in 10-30th August ICAR-IASRI, New
Biometrics 2017 Delhi
II. Administrative
17. Mr. B.L. Kokkula, Training Programme on 1-7thFebruary ICAR-NAARM,
Administrative Administration and Finance 2018 Hyderabad
Officer Management for Under Secretaries,
Sr. Administrative Officers,
SFAOs, AOs, FAOs
18. Munish N. Ganti,, National level training on 11-15th ICAR-Central Potato
Assistant Finance Procurement & PFMS for ICAR September 2017 Research Institute,
and Accounts Officers Shimla
Officer
Training programme on ICAR- 19-24th January At ICAR-NIASM,
ERP (MIS & FMS) 2017 Baramati by IASRI,
New Delhi
Management Development 22-27th June 2015 National Institute of
Programme on Accrual Accounting Financial
in Autonomous Bodies Management,
Faridabad
19. Mrs. Pallavi CSP for Assistants (DR) of ICAR 31st December Institute of
Kataria, Assistant 2013 – 10th Secretariat Training
January 2014 and Management,
Delhi
20. Mr. P.P. Kalbhor, Training programme on ICAR- 19-24th January At ICAR-NIASM,
UDC ERP (MIS & FMS) 2017 Baramati by IASRI,
New Delhi
Implementation of NIC’s e- 27-28th April ICAR-NAARM,
Procurement solution through CPP 2016 Hyderabad
Portal for ICAR Institutes (Western
Zone)
21. Mr. V.D. Gaikwad, Training programme on ICAR- 19-24th January At ICAR-NIASM,
UDC ERP (MIS & FMS) 2017 Baramati by IASRI,
New Delhi
III. Technical
22. Mr. U.N. Borse, Sr. 3-days Orientation-cum-Training 24-27th June 2016 ICAR-NRCG, Pune
Technical Officer Programme for Technicians
Investment Opportunities in Grid- 5-6th May 2015 World Institute of
connected Solar Power in India Sustainable Energy
(WISE) at Pune
5th Capacity Building Programme 8-19th September IIPA, New Delhi
for Technical Assistants 2014
23. Mr. P.B. Jadhav, Automobile Maintenance, Road 19-23rd ICAR-CIAE, Bhopal
Technical Officer Safety and Behavioural Skills September 2017
(Batch II)

staff
24. Mr. B.B. Khade, Competency Enhancement 15-24th June 2017 ICAR-NAARM,
Technical Officer Programme on Soft Skills and Hyderabad
Personality Development for
Technical Staff of ICAR T-1 to T-5
Refresher Course on Farm 19-24th ICAR-IIFRS,
Management September 2016 Modipuram, Meerut
3-days Orientation-cum-Training 24-27th June 2016 ICAR-NRCG, Pune
Programme for Technicians
25. Ms. Shailaja Satam, Training programme on ICAR- 19-24th January At ICAR-NIASM,
Technical Officer ERP (MIS & FMS) 2017 Baramati by IASRI,
New Delhi
Networking: Basics and 25-30th July 2016 ICAR-IASRI, New
Management Delhi
Awareness workshop on usage of 16th December ICAR-IASRI, New
‘Unified Messaging Solution’ 2015 Delhi at ICAR-CIFE,
Mumbai
Office Automation using Oracle 9-13th March ICAR-IASRI, New
ERP 2015 Delhi
Workshop-cum-installation 30th August 2013 ICAR-CIFE,
training programme for NAIP Mumbai
Consortium “Strengthening
Statistical Computing for NARS”
26. Mr. B.J. Phalke, Sr. Selection, Adjustment, Operation 1-10th August ICAR-CIAE, Bhopal
Technical Assistant and Maintenance of Agricultural 2017
Implements for Field and
Horticultural Crops
Competency Enhancement 15-24th June 2017 ICAR-NAARM,
Programme on Soft Skills and Hyderabad
Personality Development for
Technical Staff of ICAR T-1 to T-5
3-days Orientation-cum-Training 24-27th June 2016 ICAR-NRCG, Pune
Programme for Technicians
27. Mr. S.S. Bhoite, Sr. 3-days Orientation-cum-Training 24-27th June 2016 ICAR-NRCG, Pune
Technical Assistant Programme for Technicians
28. Mr. E.G. Kamble, 3-days Orientation-cum-Training 24-27th June 2016 ICAR-NRCG, Pune
Sr. Technician Programme for Technicians


Annexure 9
List of Scientists on Foreign Deputation
Year Name Date of Visit Place of visit Purpose
2013-14 Dr. S.D. Sawant, 31/07/2013 to Spain and Italy With the delegation of Maharashtra State
Director 04/08/2013 Grape Growers Association.
Dr. S.D. Sawant, 05/02/2014 to Logistica With the delegation of Maharashtra State
Director 09/02/2014 Exhibition, Grape Growers Association
Berlin, Germany
Dr. K. Banerjee, 25/08/2013 to Chicago, USA To participate in 127th AOAC International
Pr. Scientist 28/08/2013 2013 Annual Meeting & Exposition.
2014-15 Dr. K. Banerjee, 10/08/2014 to San Francisco, To participate in 13th IUPAC (International
Pr. Scientist 15/08/2014 California, USA Union of Pure and Applied Chemistry)
International Congress on Pesticide
Chemistry.
2015-16 Dr. K. Banerjee, 13/04/2015 to Malaysia To participate in Programme Advisory
Pr. Scientist 17/04/2015 Committee meeting of Malaysian Palm Oil
Board as a member.
2016-17 Dr. K. Banerjee, 11/04/2016 to Malaysia To participate in Programme Advisory
Pr. Scientist 15/04/2016 Committee meeting of Malaysian Palm Oil
Board as a member.
2017-18 Dr. S. D. Sawant, 30/07/2017 to ARO, Israel To attend an exhibition of patented varieties
Director 03/08/2017 and new promising hybrids of table grapes
and to attend a meeting for finalizing the
collaboration between ARO, Israel and
Maharashtra State Grape Growers
Association as a technical expert.
Dr. K. Banerjee, 03/04/2017 to Kuala Lumpur, To participate in Programme Advisory
Pr. Scientist 07/04/2017 Malaysia Committee meeting of Malaysian Palm Oil
Board as a member.
Dr. K. Banerjee, 14/05/2017 to Costa Rica To participate in 6th Latin American
Pr. Scientist 17/05/2017 Pesticide Residue Workshop (LAPRW 2017)
Dr. K. Banerjee, 24/09/2017 to Atlanta, Georgia, To participate in 131st AOAC International
Pr. Scientist 28/09/2017 USA. Annual Meeting and Exposition


Annexure 10
Participation of scientists in seminar/symposia/conferences
International
Name of the scientists Title Period Organizer and place
Dr. S.D. Sawant 6th International Conference 23-27th Indian Phytopathological
Dr. Indu S. Sawant on ‘Plant, Pathogens and February 2016 Society, New Delhi
Dr. Ahammed Shabeer People’
T.P.
Dr. S.D. Sawant International Seminar 14-16th VNMKV, Parbhani; MPKV,
‘Global Climate Change: December Rahuri; Dr.PDKV, Akola;
Implications for Agriculture 2017 Dr.BSKKV, Dapoli; and
and Water Sectors’ Water and Land
Management Institute
(WALMI), Aurangabad at
WALMI, Aurangabad
Dr. Indu S. Sawant and International Symposium on 16-18th Babasaheb Bhimrao
Dr. A.K. Sharma Innovations in Horticulture October 2014 Ambedkar University,
for Nutritional Security, Lucknow
Conserving Biodiversity and
Poverty Alleviation
Dr. Anuradha Upadhyay Next-Gen Genomics & 17-19th National Institute of Mental
Bioinformatics Technologies November Health and Neuro Sciences,
(NGBT) Conference 2014 2014. Bengaluru
Dr. K. Banerjee 3rd Annual Conference of the 19-20th AOAC International India
India Section of AOAC November Section at Pune
International 2015
Dr. S.D. Ramteke 25th Asian-Pacific Weed 13-16th Indian Society of Weed
Science Society Conference October 2015. Science at Hyderabad.
on Weed Science for
Sustainable Agriculture,
Environment and
Biodiversity
Dr. S.D. Ramteke 3rd International Plant 11-14th Indian Society for Plant
Physiology Congress December Physiology, JNU and
Challenges and Strategies in 2015 NIPGR
Plant Biology Research
Dr. S.D. Ramteke IJTA 1st International 6-7th June Academic Research
Conference on ‘Agriculture 2015 Journals, New Delhi.
and Horticulture Sciences
Dr. A.K. Sharma, Dr. International Symposium on 5-8th ICAR, New Delhi; ICAR-
D.S. Yadav, Dr. Roshni Horticulture: Priorities and September IIHR, Bengaluru; Society for
R. Samarth, Ms. Emerging Trends 2017 Promotion of Horticulture,
Sharmistha Naik ICAR-IIHR, Bengaluru and
International Society for
Horticultural Science,
Belgium at Bengaluru

rd th
Dr. Sujoy Saha 3 International Symposium 9-12 ICAR-CPCRI at ICAR-
on Phytophthora: September IIHR, Bengaluru
Taxonomy, Genomics, 2015
Pathogenicity, Resistance
and Disease Management
Dr. D.S. Yadav Third International 8-11th RKM Foundation, SBSM,
Conference on Bioresource November ICAR and Viswa-Bharati
and Stress Management 2017 University at Jaipur,
Rajasthan
Dr. Amala U. International Conference: 27-29th Entomological Research
Changing scenario of pest November Association, Udaipur and
problems in agri-horti 2014 Department of Entomology,
ecosystem and their Rajasthan College of
management’ Agriculture, MPUAT,
Udaipur
Dr. Amala U. International Conference on 27-30th Agricultural College and
‘Innovative Insect January 2015 Research Institute (TNAU),
Management Approaches for Madurai
Sustainable Agro-Ecosystem
Ms. Anupa T. International Conference on 2nd - 3rd Society for Promotion of
Vertical Farming November Horticulture and ICAR-
2015 IIHR, Bengaluru
National
Dr. S.D. Sawant and Dr. National Level Conference 26-27th Karnataka Wine Board,
A.K. Sharma on WINE December 2013 Bengaluru
Dr. S.D. Sawant ‘National Symposium (IPS, 28-29th IPS, New Delhi;
(participated as the Chief West Zone) on ‘Prospects in November 2015 VNMKV, Parbhani; and
Guest) Diversity, Diagnosis and Badrinarayan Barwale
Dr. Indu S. Sawant Management of the Diseases College, Jalna
of Horticultural and Field
Crops’
Dr. S.D. Sawant Interactive meeting of Vice- 23rd – 24th ICAR, New Delhi
Chancellors of AUs with January 2016
Directors of ICAR Institutes
and ICAR Director’s
Conference
Dr. S.D. Sawant Conference of Vice- 15th-16th May ICAR at NASC, New
chancellors of SAUs and 2015 Delhi
Directors of ICAR Institutes
Dr. S.D. Sawant Take it to Farmers – The 7th July 2015 PPVFRA
Farmers’ Rights through
Awareness
Dr. S.D. Sawant 87th ICAR Foundation Day 25-26th July Patna
and Award Ceremony and 2015
National Conference of
KVKs

Dr. S.D. Sawant National Seminar on Chitin 29th September Society of Fisheries
Dr. Indu S. Sawant in Agriculture, Medicine and 2015 Technologists (India)
Allied fields (SOFTI), NAAS, ICCS
and Central Institute of
Fisheries Technology,
Cochin
Dr. S.D. Sawant Sakal AgroWon Horticulture 06/11/2016 Nasik
Conference and Exhibition
Dr. S.D. Sawant 7th Indian Horticultural 15-18th New Delhi
Dr. Indu S. Sawant Congress 2016: Doubling November 2016
Dr. R.G. Somkuwar Farmers Income through
Dr. Anuradha Upadhyay Horticulture”
Dr. A.K. Upadhyay
Dr. K. Banerjee
Dr. S.D. Ramteke
Dr. A.K. Sharma
Dr. Sujoy Saha
Dr. S.D. Sawant Director’s Conference 7-8th March 2018 ICAR, New Delhi
Dr. Indu S. Sawant International Conference: 30-31st January Association for the
Biodiversity, Bioresources 2014 Advancement of
and Biotechnology’ Biodiversity Science;
Society for Applied
Biotechnology; Imperial
Scientific Publishing
organized at Mysore.
Dr. Indu S. Sawant International Conference on 25-27th Dr. D.Y. Patil
Advances in Biotechnology November 2013 Biotechnology and
and Bioinformatics 2013 Bioinformatics Institute
and Biotech Research
Society, India at Pune
Dr. Indu S. Sawant National Citrus Meet 12 – 13th August PPV&FRA and NRC for
2013 Citrus, Nagpur
Dr. Indu S. Sawant Conference on sustainable 27th February Protein Foods & Nutrition
solutions in food industry. 2015 Development Association
gave a presentation on ‘New of India, Mumbai
grape juice variety,
MEDIKA, from ICAR-
NRCG ’ for introducing the
variety to the participants
from food industry
Dr. Indu S. Sawant National Symposium on 16-17th March Indian Phytopathological
Understanding Host- 2015 Society, New Delhi at
pathogen Interactions IISR, Calicut
Through Science of Omics
Dr. Indu S. Sawant and Conference on sustainable 27th February Protein Foods & Nutrition
Dr. A.K. Sharma solutions in food industry 2015 Development Association
of India, Mumbai

th
Dr. Indu S. Sawant National Symposium on 9-11 January ICAR Research Complex
‘Diagnosis and Management 2017 for NEH Region, Umiam,
of Plant Diseases: Integrated Meghalaya
Approaches and Recent
Trends’
Dr. Indu S. Sawant Special symposium on 5-7th October B.A. College of
‘Microbial Antagonists and 2017 Agriculture, Anand,
their Role in Biological Gujarat
Control of Plant Diseases' &
West Zone Meet of IPS –
2017
Dr. R.G. Somkuwar 6th Indian Horticulture 6-9th November Horticultural society of
Dr. Anuradha Upadhyay Congress 2014 2014 India in coordination with
Dr. A.K. Upadhyay Tamil Nadu Agricultural
Dr. K. Banerjee University (TNAU) at
Dr. S.D. Ramteke TNAU, Coimbatore
Dr. J. Satisha
Dr. A.K. Sharma
Dr. Roshni R. Samarth
Dr. Anuradha Upadhyay National Seminar of 14th June 2013 Indian Institute of
Horticulture Biotechnology Horticultural Research,
2013. Bengaluru
Dr. Anuradha Upadhyay National Seminar on February 18-20, cDAC, Pune
"Accelerating Biology 2014: 2014
Computing Life".
Dr. Anuradha Upadhyay National Conference on 27-29th April ICAR-IIHR, Bengaluru
Dr. Roshni R. Samarth "Fruit Breeding in Tropics 2016
and Subtropics - An Indian
Perspective"
Dr. K. Banerjee and Dr. National Symposium on 28-30th January Society of Pesticide
Ahammed Shabeer T.P. Agrochemicals for Food and 2015 Science India
Environment Safety
Dr. K. Banerjee Strategies in Plant 3-5th March 2016 Institute of Agricultural
Physiological Research for Sciences, Banaras Hindu
meeting Challenges in University, Varanasi
Agriculture
Dr. K. Banerjee 12th National Symposium 17/02/2017 to Uttar Banga Krishi
on ‘Biotic stress 19/02/2017 Viswavidyalaya (UBKV)
management: challenges and and Society for Plant
environmental Protection Sciences,
harmonization’
Dr. K. Banerjee 5th Annual Conference of 28th February – India Section of AOAC
the India Section of AOAC 1st March 2018 International at New Delhi
International
Dr. S.D. Ramteke ‘National Conference of 13-16th Directorate of Groundnut
Plant Physiology-2013 on December 2013 Research, Junagadh and
Current Trends in Plant Indian Society for Plant
Biology Research’. Physiology, New Delhi at
DOGR, Junagadh

th
Dr. S.D. Ramteke National Conference of Plant 23-25 Orissa University of
Physiology-2014 on November 2014 Agriculture &
Frontiers of Plant Technology,
Physiology Research: Food Bhubaneswar and Indian
Security and Environmental Society for Plant
Challenges Physiology, New Delhi
Dr. S.D. Ramteke National Conference of Plant 08/12/2016 to University of Agricultural
Physiology 10/12/2016 Sciences, Bengaluru and
Indian Society for Plant
Physiology, New Delhi
Dr. J. Satisha National Seminar on 11-12th CPCRI, Kasaragod
Application of November 2013
Bioinformatics in
Agriculture
Dr. A.K. Sharma, Dr. Emerging trends in high-tech 6-7th March ICAR-Central Institute of
D.S. Yadav horticulture under changing 2018 Temperate Horticulture,
climate Regional Station,
Mukteshwar
Dr. Sujoy Saha National Symposium on 23-24th Indian Phytopathological
Challenges and Perspective November 2017 Society (East Zone) and
in Plant Health Managemetn Bidhan Chandra Krishi
under Climate Change Viswavidyalaya
Scenario
Dr. Ahammed Shabeer T. National Seminar on ‘Soil 8-9th May 2013 Dapoli Chapter of Indian
P. Fertility, Degradation and Society of Soil Science,
Contaminants’. Dr. Balasaheb Sawant
Konkan Krishi
Vidyapeeth, Dapoli
Dr. Ahammed Shabeer Seminar on ‘Food Business 25/10/2016 Bombay Chamber of
T.P. in India – Emerging Trends’ Commerce & Industry
Dr. Ahammed Shabeer SAARC-PTB workshop on 25/07/2016 to National Accreditation
T.P. capacity building for CRM 26/07/2016 Board for Certification
Bodies, New Delhi


Annexure 11
Budget details for five years
Sl. R.E. 2013-14 Expenditure 2013-14 Final Grant
Heads
No. Plan Non-Plan Plan Non-Plan Plan Non-Plan
1. Estt. Charges 0.00 330.00 0.00 293.82001 0.00 330.00
3. O.T.A. 0.00 0.00 0.00 0.00 0.00 0.00
4. T.A. 10.00 1.50 10.00 1.5 10.00 1.5
5. Equipment 60.00 4.00 51.98358 4.00 60.00 4.00
6. Library books 0.114 0.00 0.11390 0.00 0.114 0.00
5. Other charges 237.00 80.00 236.24470 79.99386 237.00 80.00
6. Works 35.386 10.00 33.28425 10.00 35.386 10.00
7. Furniture 37.50 1.00 37.49703 1.00 37.50 1.00

8. Pension 0.00 0.00 0.00 30.00 0.00 30.00
Total 380.00 456.50 380.00 390.24106 380.00 456.50

Heads
1. Estt. Charges 0.00 355.00 0.00 335.00 0.00 335.00
3. O.T.A. 0.00 0.00 0.00 0.00 0.00 0.00
4. T.A. 10.00 2.00 10.00 2.00 10.00 2.00
5. Equipment 44.86 3.00 44.86 3.00 44.86 3.00
6. IT 14.99 0.00 14.99 0.00 14.99 0.00
6. Library books 0.15 0.00 0.15 0.00 0.15 0.00
5. Other charges 196.14 160.00 196.14 78.00 196.14 78.00
6. Works 4.86 0.00 4.86 0.00 4.86 0.00
7. Furniture 0.00 2.00 0.00 2.00 0.00 2.00
8. Pension 0.00 20.00 0.00 0.00 0.00 0.00
Total 271.00 542.00 271.00 420.00 271.00 420.00

Heads
1. Estt. Charges 0.00 400.00 0.00 360.34 0.00 370.00
3. O.T.A. 0.00 0.10 0.00 0.05 0.00 0.10
4. T.A. 10.00 2.00 10.00 2.00 10.00 2.00
5. Equipment 54.23 3.00 54.23 2.99 54.23 3.00
6. IT 2.46 0.00 2.46 0.00 2.46 0.00
6. Library books 1.44 0.00 1.44 0.00 1.44 0.00
5. Other charges 52.00 222.90 51.98 222.82 52.00 121.90
6. Works 135.93 0.00 135.93 0.00 135.93 0.00
7. Furniture 0.00 2.00 0.00 2.00 0.00 2.00
8. Pension 0.00 7.00 0.00 1.88 0.00 7.00
Total 256.06 637.00 256.04 592.08 256.06 506.00

Heads
1. Estt. Charges 0.00 416.26 0.00 404.93 0.00 416.26
3. O.T.A. 0.00 0.10 0.00 0.00 0.00 0.10
4. T.A. 5.84 3.00 5.84 3.00 5.84 3.00
5. Equipment 17.20 4.00 17.20 3.99 17.20 4.00
6. IT 1.53 0.00 1.53 0.00 1.53 0.00
6. Library books 1.43 0.00 1.43 0.00 1.43 0.00
5. Other charges 72.26 252.49 72.26 252.48 72.26 252.49
6. Works 127.87 0.00 127.87 0.00 127.87 0.00
7. Furniture 15.97 2.00 15.97 2.00 15.97 2.00
8. Pension 0.00 73.15 0.00 49.19 0.00 73.15
Total 242.10 751.00 242.10 715.59 242.10 751.00

Heads
No. Institute Institute Institute
1. Estt. Charges 475.00 461.25 475.00
3. O.T.A. 0.10 0.00 0.10
4. T.A. 13.00 13.00 13.00
5. Equipment 0.00 0.00 0.00
6. IT 0.00 0.00 0.00
6. Library books 0.00 0.00 0.00
5. Other charges 409.75 409.69 409.75
6. Works 7.00 7.00 7.00
7. Furniture 0.00 0.00 0.00
8. Pension 39.25 22.58 39.25
Total 944.10 913.52 944.10


Annexure 12
Details of revenue generation
S. No. Year Revenue generated
(₹ lakhs)
1. 2013-14 46.86995
2. 2014-15 57.08
3. 2015-16 59.89914
4. 2016-17 76.39
5. 2017-18 86.17
TOTAL 326.40909


Annexure 13
Details of Consultancy Projects
Title of the consultancy project Agency From To Project Cost
1. Guiding the grape growers on grape Rallis India 14/11/2014 14/11/2014 20226
management after October pruning in Limited
the grape seminar organized at
Pimpalgaon Baswant, district Nasik
2. Guiding grape growers on water and Maharashtra 15/11/2014 15/11/2014 20226
nutrient management, disease and Chamber of
insect management in grapes in farmers Commerce,
seminar at Nasik Industry and
Agriculture
3. Guiding the grape growers on grape ATMA, Solapur 25/11/2014 25/11/2014 19101
pest and disease diagnosis and
management in the current situation in
the Charchasatra organized at Barshi
district Solapur
4. Guiding the grape growers in the Krishi Vigyan 28/11/2014 29/11/2014 26967
seminar at Bori and at Sarkalwadi, Kendra,
district Pune Baramati
5. Grape Vineyard Management Agrowon, Sakal 02/12/2014 02/12/2014 6742
6. Guiding the grape growers in the crop Deepak 04/12/2014 04/12/2014 20226
seminar on grape at Pimpalgaon Fertilisers and
Baswant, district Nasik Petrochemicals
Corporation Ltd
7. Guiding grape growers in the technical Synergy 08/01/2015 08/01/2015 6742
seminar at Pimpalgaon Baswant, Biotechnologies
district Nasik
8. Grape new variety – opportunity, Sahyadri Farms, 06/08/2015 06/08/2015 12540
challenges, development in the Sahyadri Farmer
farmers’ meeting’ at Mohadi, district Producer Co.
Nasik Ltd., At Post –
Adgaon, Tal and
Dist – Nasik –
422003
9. Guiding grape growers in Charchasatra Shree Samarth 12/08/2015 13/08/2015 55860
at Palus, district Sangli Dhondiraj
Maharaj
Shetkari Mandal
10. MSI-CDP training programme Vaishnavi Grape 19/09/2015 19/09/2015 31920
organized at Kasegaon, taluka- and
Pandharpur, district-Solapur. Pomegranate
Processing Pvt.
Ltd.
11. Conducting training programme as a Mahindra 21/10/2015 21/10/2015 12540
consultant for field staff at Nasik. Shubhlabh
Services Ltd.
Nasik

12. ‘Improved package of practices of District 17/11/2015 17/11/2015 55860
grapes’ at Jalna. Superintendent
Agricultural
Officer, Jalna
13. National Conference on Mass Indian Institute 30/11/2015 30/11/2015 12540
Spectrometry Technology and of
Emerging Applications as a Chromatography
Consultancy Programme and Mass
Spectrometry
(IICMS)
14. Guiding the grape exporter farmers in District 21/12/2015 21/12/2015 37785
the workshop Superintendent
Agricultural
Officer, Sangli
15. Guiding grape growers in grape Shree Swami 23/01/2016 23/01/2016 25190
management seminar in World Samarth Krushi
Agriculture Festival 2016 at Nasik Vikas
Sanshodhan
Charitable Trust
16. Guiding grape growers in the meeting Bayer 18/04/2016 18/04/2016 36640
CropScience
Limited
17. Guiding grape growers on ‘Effective Dabholkar 16/08/2016 16/08/2016 12650
disease control and residue Prayog Pariwar
management with less number of
sprays’ at Pimpalgaon Baswant, district
Nasik
18. Assessment of yield increase in table Mahindra 01/09/2016 31/03/2017 474160
grapes in farmers' fields through Shubhlabh
technology developed by Mahindra Services
Shubhlabh
19. Participation as a consultant in one day Tropical 06/09/2016 06/09/2016 12650
consultancy programme for company Agrosystems (I)
staff Pvt. Ltd.
20. Participation as resource person for the Fairtrade - 15/09/2016 15/09/2016 6900
training programme organized at Nasik Network of Asia
and Pacific
Producers
21. Addressing grape farmers in Netafim 23/09/2016 23/09/2016 25300
‘Fertigation Automation awareness Irrigation India
programme’ at Tasgaon Pvt. Ltd.
22. Guiding grape growers on ‘October Gramsamruddhi 06/10/2016 06/10/2016 12650
pruning in grape vineyard and Foundation,
management thereafter’ in the Grape Nasik
Seminar organized at Nasik
23. Delivering lecture on ‘Recent trends in Krishi Vigyan 06/10/2016 06/10/2016 12650
disease management in grapes’ in the Kendra, Nasik
farmers rally organized by KVK Nasik
at Pimpalgaon (Baswant)

24. Delivering lectures to the farmers Yashwantrao 17/10/2016 17/10/2016 25300
gathering at Nasik Chavan Social
Forum
Nasik
25. Guiding the grape growers in the grape District 24/10/2016 24/10/2016 43700
workshop being organized at Jalna Superintendent
Agricultural
Officer, Jalna
26. Expert guidance to grape growers for Bayer 22/11/2016 23/11/2016 66850
increasing productivity and quality of CropScience
grapes Limited
27. Guiding the grape growers in the grape Krishna Valley 17/12/2016 17/12/2016 25300
Charchasatra organized at Kasegaon, Agrotech LLP,
Pandharpur Pune
28. Sustainable agricultural practices in IDH, The 10/01/2017 31/01/2017 145689
table grapes in Maharashtra Sustainable
Trade Initiative
29. To give a presentation in the seminars Thermo Fisher 24/01/2017 27/01/2017 25300
as a Consultancy Programme Scientific
30. Guiding the grape growers in the Krishak Bharati 10/02/2017 10/02/2017 25300
seminar organized at Puluj, Tal. Cooperative Ltd.
Pandharpur.
31. Technical advice on viticultural Divya 15/04/2017 15/04/2017 11500
practices for current scenario Bioscience
32. Participating in the Rallis Scientific Rallis India 01/06/2017 01/06/2017 17250
Advisory Meet at Lonavala Limited
33. To give a presentation in a seminar Agilent 06/06/2017 06/06/2017 12650
organized by Agilent Technologies as a Technologies
consultancy programme India Pvt. Ltd.
34. Expert guidance on viticultural aspects Bayer 15/07/2017 30/08/2017 556960
for mobile application. CropScience
Limited
35. Guiding grape growers in the grape Mahatma Phule 21/07/2017 21/07/2017 50740
Charchasatra at Indapur, dist. Pune. Krishi Vikas
Kendra
36. Expert guidance to grape growers for Bayer 08/08/2017 31/10/2017 69620
increasing productivity and quality of CropScience
grapes Limited
37. Guidance to (i) consultants working on Isha Agro India 09/08/2017 09/08/2017 23010
grapes from Vijapur and Athan,
Karnataka and (ii) sales team working
in the grape growing region of
Maharashtra and Karnataka in the
training programme organized at
ICAR-NRCG, Pune
38. Guiding grape growers on nutrient and Rallis India 17/08/2017 17/08/2017 12980
water management in the crop seminar Limited
organized at Baramati

39. Training to raisin making group of District 22/08/2017 22/08/2017 48380
grape producers on (i) nutritional Industries
management and (ii) residue free Centre
grapes and raisin production at Osmanabad
Osmanabad
40. Participation in the farmers’ training Coromandel 13/09/2017 13/09/2017 25960
programme as guest speakers International
organized at Dindori, district Nasik Ltd.
41. Rationalizing input use to increase Indian Society 01/12/2017 31/03/2020 1680320
quality grape production. of Agribusiness
Professionals
42. Participation in a technical discussion Bekaert 28/02/2018 28/02/2018 12980
on grapes trellis system organized at Industries Pvt.
Nasik. Ltd.


Annexure 14
Contract research trials conducted
s. Title of the contrract research trial Duration Sponsored by Charges
no. From To (Rs.)
1 Bio efficacy and Phytotoxicity evaluation of 01-10- 30-11- Maktheshim 373035
Azoxystrobin 23%w/wSC in control of Downy 2012 2013 Agan India
mildew, and powdery mildew in Grapes after fruit Pvt. Ltd.
pruning
2 To test the bio-efficacy of Trichoderma-shield Combat 01-10- 30-11- Kan Biosys 178091
(Trichoderma Viride 1.15% A.S.) in bio-management 2012 2013 Pvt. Ltd.
of post harvest berry rots in grapes
3 Bio-efficacy and phytotoxicity of Pyraclostrobin 01-10- 30-11- BASF India 261411
12.8% + Boscalid 25.2% WG (Pristine 38% WG) 2012 2013 Limited
against Powdery mildew disease of Grapes
4 To evaluate the bioefficacy of AURA XL in grape 01-08- 31-07- Coromandel 303821
2013 2014 Agrico Pvt.
Ltd.
5 Evaluation of bio-efficacy of Brigade B (Beauveria 01-10- 30-09- Kan Biosys 120000
bassiana 1.15% AS) against mealybug in grapes 2012 2013 Pvt. Ltd.
6 Bio-efficacy, phytotoxicity & residue of study the 01-10- 30-11- Privi Pharma 178091
bioefficacy of Privi Nutrifight on Grapes in control of 2012 2013 Pvt. Ltd.
Downy mildew & Powdery mildew in Grapes
7 To study the effect of Silixol in combination with 01-10- 30-11- Privi Pharma 234271
different levels of pesticides on Grapes desease & 2012 2013 Pvt. Ltd.
residue monitoring
8 To study the effect of CROPSIL on growth, 01-05- 30-09- Lifeline 179776
fruitfulness, yield and quality of Thompson Seedless 2013 2014 Technologies
grapes
9 Bioefficacy of Pinknil against pink berries in grapes 01-10- 30-11- Elantass Agro 159341
2013 2014 Pvt. Ltd.
10 Bioefficacy of Elanta Super and its terminal residues 01-10- 30-11- Elantass Agro 446562
in grapes 2013 2014 Pvt. Ltd.
11 Bioefficacy of Cynoboost Super nano in relation to 01-10- 30-11- Elantass Agro 280900
even sprouting in grapes 2013 2014 Pvt. Ltd.
12 Evaluation of bioefficacy of Soaker against mealybug 01-10- 30-09- Elantass Agro 200000
in grapes 2013 2014 Pvt. Ltd.
13 Bioefficacy, phytotoxicity and residue of 01-09- 30-11- Dhanuka 921352
Kasugamycin 5% + Copper Oxychloride 45% WP 2013 2015 Agritech Ltd.
(Conika) in control of bacterial blight, and anthracnose
in Grapes after forward pruning
14 Bioefficacy, phytotoxicity and residue of Kirari 20% 01-09- 30-11- Dhanuka 1067420
SC (Amisulbrom) in control of downy mildew in 2013 2015 Agritech Ltd.
Grapes after forward pruning
15 Bioefficacy, phytotoxicity and residue of Cyflunamid 01-09- 30-11- Dhanuka 1067420
5% EW in control of powdery mildew in Grapes after 2013 2015 Agritech Ltd.
forward pruning

no. From To (Rs.)
16 Bioefficacy, phytotoxicity and residue of CHA 1322 01-10- 30-11- Cheminova 280900
(Flutrifol 250g/l SC) in control of powdery mildew in 2013 2014 India Ltd.
17 Bioefficacy of Bacillus subtilis based biofungicide, Novozymes 606744
Taegro in control of downy mildew, powdery mildew South Asia
and anthracnose in grapes after forward pruning Pvt. Ltd.
18 To evaluate the bioefficacy of Pro-gibb in grape 01-08- 31-07- Sumitomo 382024
2013 2014 Chemical India
Pvt. Ltd.
19 Evaluation of Metarhizium anisopliae against thrips, 01-10- 30-06- Novozymes 337080
leafhopper, mite and stem borer in grapes 2013 2015 South Asia
Pvt. Ltd.
20 Evaluation of physiological effects of Cabrio Top 60% 01-10- 30-06- BASF India 370788
WG (Metiram 55% + Pyraclostrobin 5% WG) in grape 2012 2013 Limited
21 Residue studies of Chlorothalonil 75 WP applied as 01-12- 01-04- Syngenta India 67416
foliar spray in grapes after forward pruning 2013 2014 Limited
22 Evaluation of bioefficacy of Spirotetramate 150 OD 01-10- 30-09- Bayer 390000
(w/v) against mealybug and mite in grapes 2013 2014 CropScience
Limited
23 Bioefficacy, phytotoxicity and residue of fluxapyroxad 01-11- 30-11- BASF India 443822
7.5% + Difenconazole 5% (BAS 717 00F 12.5%) SC 2013 2014 Limited
in control of powdery mildew in grapes after forward
pruning
24 Analysis of 2-phenyl phenol and different pesticide 01-02- 30-01- Isagro (Asia) 61798
content in bio-product samples by GC-MS/MS or LC- 2014 2015 Agrochemicals
MS/MS instrument Pvt. Ltd.
25 Fluopicolide 4.44 + Fosetyl Al 66.66-71 WG (Profiler 01-02- 30-04- Bayer 651688
71.14 WG) supervised residue and persistence trials in 2014 2015 CropScience
grapes Limited
26 Bioefficacy of Ethrel 39 SL in grapes for defoliation 01-10- 30-06- Bayer 303372
2013 2014 CropScience
Limited
27 Evaluation of bioefficacy of Spinetoram 12% SC 01-10- 30-09- Dow 337080
(11.7% w/w) against flea beetle, thrips and 2013 2014 AgroSciences
lepidopteron pests in grapes India Pvt. Ltd.
28 To study the effect of DRAKSHATAR on growth, 01-04- 31-03- Austenitic 252810
yield and quality parameters in Thompson Seedless 2014 2015 Steels Private
grape Limited
29 To study the bioefficacy and residue dissipation of 20-01- 31-03- Omega Fine 595508
Rescue-D (Chitosan formulation) against powdery 2014 2015 Chemicals
mildew in grapes and its effect on degradation kinetics
of myclobutanil and difenconazole
30 Bio-efficacy and phytotoxicity of Chitosan (Kitosan 01-09- 30-11- Vaishnavi 337080
10%) in control of Powdery mildew in Grapes after 2014 2015 Biotech
forward pruning Limited

no. From To (Rs.)
31 To study the residue dissipation of Swell 0.1% 01-07- 30-04- Omega Fine 374160
(CPPU) in orange, mango, cotton seed and soil 2014 2015 Chemicals
32 Evaluation of Met 52 EC, Metarhizium anispoliae 01-10- 30-06- Novozymes 533710
against thrips, leafhopper, mealybugs and mites in 2014 2015 South Asia
grapes Pvt. Ltd.
33 Bioefficacy of Streptomyces lydicus based 01-10- 30-11- Novozymes 494384
biofungicide, Actinovate AG in control of downly 2014 2015 South Asia
mildew and powdery mildew in grapes after forward Pvt. Ltd.
pruning
34 Bioefficacy of Bacillus subtilis based biofungicide, 01-10- 30-11- Novozymes 870790
Taegro in control of downy mildew and powdery 2014 2015 South Asia
mildew in grapes after forward pruning Pvt. Ltd.
35 Bioefficacy, phytotoxicity of Proquinazid 20% EC in 01-10- 30-09- E.I. DuPont 303372
control of powdery mildew in grapes after forward 2014 2015 India Pvt. Ltd.
pruning
36 To study the strength, durability and load bearing 01-04- 31-03- Superfil 286520
capacity of Agrolines wire using Thompson Seedless 2014 2015 Products
grapevine Limited
37 Evaluation trial for the efficacy of Divya Amrut and 01-10- 30-09- Divya 387642
Divya Soil Conditioner on growth, yield and quality of 2014 2015 Bioscience
grape
38 Evaluation of Propargite (50%) and Bifenthrin (5%) 01-11- 30-10- Indofil 1112364
residues in tomato and okra 2014 2015 Industries
Limited
39 Bio-efficacy, Phytotoxicity and Residue of 01-11- 30-11- BASF India 443822
Fluxapyroxad 7.5% + Difenconazole 5% (BAS 717 2014 2015 Limited
00F 12.5%) SC in control of powdery mildew in
40 To study the bio-efficacy of Cabrio Top in Grapes 01-10- 30-09- BASF India 370788
2014 2015 Limited
41 To study the effect of StopIT formulation on yield and 01-11- 30-04- Yara Fertilisers 297754
quality of grape 2014 2015 India Private
Limited
42 Bio-efficacy of antifeedant botanical formulation 01-12- 31-05- Farmex Agro 240450
SAMRATPLUS (Mask) against thrips infesting grapes 2014 2015 science
43 Evaluation of crop protection molecules for residue 01-06- 31-05- Rallis India 1759557
studies (Objective: Evaluation of Taqat 75 WP 2014 2015 Limited
(Hexaconazole 5% + Captan 70%) and Ergon
(Kresoxim methyl) in wheat, rice and cumin
44 Evaluation of bioefficacy of thiamethoxam 25% WG 01-10- 30-09- Syngenta India 324271
against mealybug under field conditions in grapes 2014 2015 Limited
45 Evaluation of bioefficacy of Econeem Plus (1000 ppm 01-10- 30-11- PJ Margo 377980
Azadirachtin) against thrips and mites under field 2014 2015 Private
conditions in grapes Limited

no. From To (Rs.)
46 Evaluation of bioefficacy of thiamethoxam 25% WG 01-10- 30-11- Syngenta India 341125
against flea beetle, thrips and leafhopper under field 2014 2015 Limited
conditions in grapes
47 Evaluation of chlorantraniliprole (Ferterra 0.4 g) 01-12- 30-11- E.I. DuPont 1242028
against stem borer, Celosterna scabrator F. in grapes 2014 2015 India Pvt. Ltd.
48 To evaluate bioefficacy, phytotoxicity and residue of 01-10- 30-11- Cheminova 308990
CHA 1322 (Flutrifol 23% w/w SC) in control of 2014 2015 India Ltd.
powdery mildew in grapes after forward pruning
49 Evaluation of Spinetoram 10% w/w + Sulfoxaflor 01-10- 30-09- Dow 904948
30% w/w WG against thrips, leafhopper and mealybug 2014 2015 AgroSciences
in grapes India Pvt. Ltd.
50 Evaluation of bioefficacy of Spirotetramate 150 OD 01-11- 31-12- Bayer 489216
(w/v) against mealybug and mite in grapes 2014 2015 CropScience
Limited
51 To evaluate bioefficacy, phytotoxicity and residue of 01-10- 30-11- Maktheshim 406743
Custudia (Azoxystrobin 11% + Tebuconazole 18.3%) 2014 2015 Agan India
SC in control of downy mildew and powdery mildew Pvt. Ltd.
in grapes after forward pruning
52 Evaluation of crop protection molecules for residue 01-04- 31-03- Syngenta India 314608
analysis 2014 2015 Limited
53 To evaluate the efficacy of Ethephon (39% S.L.) for 01-10- 31-07- Bayer 404496
leaf fall and its subsequent effect on fruiting and yield 2014 2015 CropScience
in grapes Limited
54 Evaluation of paraquate dichloride 24% SL against 01-09- 31-07- Maktheshim 370788
weeds in grape 2014 2015 Agan India
Pvt. Ltd.
55 Evaluation of Oxyfluorfen 2.5% + Glyphosate 41% 01-09- 31-07- Maktheshim 741576
SC grass and broad leaved weeds in grape 2014 2016 Agan India
Pvt. Ltd.
56 To evaluate bioefficacy of combination fungicide 01-12- 30-11- Bayer 238877
Fluopyram 200 + Tebuconazole 200-400 SC (w/v) 2014 2015 CropScience
against postharvest diseases and shelf life in grapes Limited
57 Evaluation of zinc metalosate, Ca metalosate and 01-04- 31-03- Indofil 1062936
boron metalosate on grapes 2015 2016 Industries
Limited
58 Development and validation of multi-residue method 01-09- 31-12- Spices Board 2100000
for analysis of pesticide residues in spices 2015 2016
59 Studies on bioefficacy, physiology and phytotoxicity 01-11- 31-10- Godrej 407867
of Diamore Combine (Homobrassinoloide 0.03%) in 2014 2015 Agrovet
grape (Vitis vinifera) Limited
60 To evaluate bioefficacy and phytotoxicity of PIF 320 01-12- 30-11- PI Industries 277529
5% SC against powdery mildew disease, in grapes 2014 2015 Ltd.
after fruit pruning
61 To evaluate persistence study of Propargite 50% + 01-06- 31-05- Indofil 737539
Befenthrin 5% SE in four different types of soil and 2015 2016 Industries
three different type of water (at pH level) Limited

no. From To (Rs.)
62 To study the bioefficacy of Privi Nutrifight on grape 01-10- 30-11- Privi Life 446069
diseases 2014 2015 Science Pvt.
Ltd
63 Evaluation of Ca Essence on grapes 01-08- 31-05- Vanita 675108
2015 2016 Agrochem (I)
Pvt. Ltd.
64 To evaluate the residues and persistence of 01-09- 31-08- Bayer 410400
Imidacloprid 17.1% w/w SL on grape 2015 2016 CropScience
Limited
65 To evaluate the residues and persistence of Luna 01-09- 31-08- Bayer 759240
Experience 400SC (Fluopyram 200+Tebuconazole 2015 2016 CropScience
200SC) on grape Limited
66 To evaluate bio-efficacy and phytotoxicity of FF15-01 01-10- 30-11- FMC India 850568
against downy mildew of grapes after fruit pruning 2015 2016 Private
Limited
Limited
68 To evaluate bio-efficacy and phytotoxicity of 01-10- 30-11- ADAMA India 563227
MAFRM-08 against downy mildew of grapes after 2015 2016 Pvt. Ltd.
forward pruning
69 To evaluate bio-efficacy and phytotoxicity of CSPAI- 01-10- 30-11- ADAMA India 631969
01 against downy mildew of grapes after fruit pruning 2015 2016 Pvt. Ltd.
70 Bio-efficacy and phytotoxicity of Custodia 01-10- 30-11- ADAMA India 460878
(Azoxystrobin 11% + Tebuconazole 18.3%) SC in 2015 2016 Pvt. Ltd.
control of powdery mildew in grapes after forward
pruning
71 Evaluation of PII 405 (Tolfenpyrad 15% EC) and PIF 01-10- 30-09- PI Industries 2175120
320 5% SC (Polyoxin D Zinc Salt) residues in grapes 2015 2016 Ltd.
and soil
72 To evaluate the residue and persistence of Bayer 410400
Tebuconazole 430SC on grape CropScience
Limited
73 To evaluate bio-efficacy and phytotoxicity of 01-10- 30-11- Bayer 476154
Tebuconazole 430 SC against powdery mildew 2015 2016 CropScience
diseases in grapes Limited
74 To evaluate bio-efficacy of combination fungicide 01-12- 30-11- Bayer 450336
Fluopyram 200 + Tebuconazole 200 - 400 SC (w/v) 2015 2016 CropScience
against post-harvest diseases and shelf life in grapes Limited
75 To evaluate bio-efficacy of PII 405 15% EC against 01-10- 30-11- PI Industries 639607
downy mildew and powdery mildew disease in grapes 2015 2016 Ltd.
after fruit pruning
76 To evaluate bio-efficacy of PIF 320 5% SC against 01-10- 30-11- PI Industries 460878
powdery mildew disease in grapes after fruit pruning 2015 2016 Ltd.

no. From To (Rs.)
77 Bio-efficacy, phytotoxicity of proquinazid 20% EC in 01-10- 30-11- E.I. DuPont 460878
control of powdery mildew in grapes after forward 2015 2016 India Pvt. Ltd.
pruning
78 Bioefficacy, phytotoxicity of Paraffinic oil 99.1% 01-10- 30-11- Synergy 454768
(saviour oil) in control of powdery mildew in grapes 2015 2016 Biotechnologie
after forward pruning s
79 Evaluation of bio-efficacy of Econeem Plus (10000 01-10- 31-12- PJ Margo 525113
ppm Azadirachtin) against thrips and mites under field 2015 2016 Private
conditions in grapes Limited
80 Evaluation of PII 405 15% EC (tolfenpyrad) against 01-10- 31-12- PI Industries 798000
thrips and leafhopper in grapes 2015 2016 Ltd.
81 Evaluation of bio-efficacy of thiamethoxam 25% WG 01-10- 31-12- Syngenta India 525113
against flea beetle, thrips and leafhopper under field 2015 2016 Limited
conditions in grapes
82 Evaluation of bio-efficacy of thiamethoxam 25% WG 01-10- 31-12- Syngenta India 510863
against mealybug under field conditions in grapes 2015 2016 Limited
83 To evaluate bio-efficacy and standardization of dose 01-10- 30-11- Sumitomo 647245
of Ethaboxam 40% SC in grapes against downy 2015 2016 Chemical India
mildew after fruit pruning Pvt. Ltd.
Divya Amrut - soil conditioner on growth, yield and 2015 2016 Bioscience
quality of grape
85 Evaluation of Turbo ZS (a combination fertilizer of 01-04- 31-05- Sulphur Mills 1234050
Elemental sulphur (70%) + Zn (15%) on grapes 2016 2017 Limited
86 To evaluate bio-efficacy and phytotoxicity of Polyram 01-10- 30-11- BASF India 646633
against downy mildew and anthracnose of grapes after 2015 2016 Limited
fruit pruning
87 To Evaluate the bio-efficacy and phytotoxicity of IIF 01-10- 30-11- Indofil 646633
311 WP against downy mildew of grapes after fruit 2015 2016 Industries
pruning Limited
88 To evaluate bio-efficacy of Bacillus subtilis based 01-10- 30-11- Novozymes 631969
biofungicide, Taegro in grapes for the control of 2015 2016 South Asia
downy mildew Pvt. Ltd.
89 To evaluate bio-efficacy of Bacillus subtilis based 01-10- 30-11- Novozymes 633497
biofungicide, Taegro in grapes for the control of 2015 2016 South Asia
powdery mildew Pvt. Ltd.
90 Studies on the residues of Ferterra 0.4G 16-12- 31-03- E.I. DuPont 1901520
(Chlorantraniliprole) in grapes and soil 2015 2017 India Pvt. Ltd.
91 Studies on the residues of Cyantraniliprole (DOX- 16-12- 31-03- E.I. DuPont 2763360
HGW86) 10% w/w OD (Benevia 10% w/w) grapes 2015 2017 India Pvt. Ltd.
and soil
92 Evaluation of BAS 450 01 I 300 SC against thrips in 01-10- 31-12- BASF India 653362
grapes 2015 2016 Limited

no. From To (Rs.)
93 Bioefficacy and phytotoxicity of Amrut Out (botanical 01-10- 30-11- Krishna Valley 537104
fungicide) in control of powdery mildew and downy 2015 2016 Agro India
mildew and Amrut powdery fighter (botanical Ltd.
fungicide) in control of powdery mildew in grapes
after forward pruning.
94 Bioefficacy studies of physio-activator Optene and 01-11- 31-10- Arysta Life 841491
Bio-stimulant (Pilatus) on improving grape quality 2015 2016 Science India
Limited
95 Studies on bioefficacy, physiology and phytotoxicity Godrej 407867
of Diamore Combine (Homobrassinoloide 0.03%) in Agrovet
grape (Vitis vinifera) Limited
96 To evaluate spray positioning of Fluopyram 200 + 01-10- 30-04- Bayer 615623
Tebuconazole 200-400 SC (w/v) in combination with 2015 2016 CropScience
Trifloxystrobin 25 + Tebuconazole 50-75 WG and Limited
biological against powdery mildew in grapes
97 Evaluation of chlorantraniliprole (Ferterra 0.4 g) 01-10- 31-12- E.I. DuPont 1610250
against stem borer, Celosterna scabrator F. in grapes 2015 2016 India Pvt. Ltd.
98 Evaluation of UPH 210b (Pyrazosulfuron ethyl 1% + 01-11- 30-11- UPL Limited 1969920
Pendimethal in 45%ZC) residues in paddy, soil and 2015 2016
water
99 Evaluation of crop protection molecules for residue 01-11- 30-11- UPL Limited 2216160
studies 2015 2016
100 Evaluation of Glufosinate ammonium 13.5%SL 01-11- 30-11- UPL Limited 718200
residues in cotton, tea and soil 2015 2016
10 Curative effect of Agro-Rakshak on powdery mildew 01-02- 31-12- Aquamarine 226234
of grapes and shelf life of grapes in post veraison stage 2016 2016 Liqua Chem
Pvt. Ltd.
102 Evaluation of Spinetoram 10% w/w + Sulfoxaflor 01-10- 31-12- Dow 996788
30% w/w WG against thrips, leafhopper and mealybug 2015 2016 AgroSciences
in grapes India Pvt. Ltd.
103 To evaluate the residues and persistence of Luna 20-12- 19-12- Bayer 1145000
Experience 400SC (Fluopyram 200+Tebuconazole 2015 2016 CropScience
200SC) on grape Limited
104 To evaluate the residues and persistence of Kresoxim 01-01- 31-12- Atul Ltd. 528990
methyl 44.3% w/w (500 g/L) SC on grape 2016 2016
105 Residue and persistence study of Dimethomorph 01-01- 31-12- ADAMA India 2958680
11.3% + Folpet 60% WG (MAFRM-08) and copper 2016 2016 Pvt. Ltd.
sulphate Pentahydrate 6% SC (CSPAI-01) on grape
106 Effect of Akshay Dip Super on grape drying and raisin 01-02- 30-09- West Coast 283960
quality 2016 2016 Herbochem
Ltd.
107 To evaluate bioefficacy and phytotoxicity of kresoxim 01-10- 30-04- Atul Ltd. 475542
methyl 44.3% SC (500% g/L) against powdery 2015 2016
mildew diseases in grapes

no. From To (Rs.)
108 A) Study of bio-product SIAPTON 10L for shelf-life 01-04- 30-03- Isagro (Asia) 103050
by accelerated storage method. B) Study of bio- 2016 2017 Agrochemicals
product SIAPTON 10L for quality evaluation for Pvt. Ltd.
inorganic contents and plant growth regulators
109 Bioefficacy studies on phosphorous solubilizing flora 01-11- 31-05- Embio Ltd. 667249
in improving grape quality 2015 2016
110 Analytical method validation of aflatoxin and 01-04- 31-03- R-Biopharm 229000
ochratoxin in spices 2016 2017 Neugen Pvt.
Ltd.
111 Evaluation of persistence and harvest residue of 01-06- 30-11- UPL Limited 2658690
Carbendazim 12% + Mancozeb 63% WP in maize and 2016 2017
Flonicamid 50% WG in mango, okra, brinjal
112 To evaluate bio-efficacy and phytotoxicity of CSPAI- 01-10- 30-11- ADAMA India 726112
01 against downy mildew of grapes after fruit pruning 2016 2017 Pvt. Ltd.
(second season)
113 To study the bio-efficacy of Silixol in grapes 01-10- 31-08- Privi Pharma 394384
2015 2016 Pvt. Ltd.
MAFRM-08 against downy mildew of grapes after 2016 2017 Pvt. Ltd.
forward pruning (second season).
115 To evaluate bio-efficacy of PII 405 15% EC against 01-10- 30-04- PI Industries 760485
downy mildew and powdery mildew disease, in grapes 2016 2017 Ltd.
after fruit pruning
116 Bio-efficacy and phytotoxicity studies on Indaziflame 01-06- 31-05- Bayer 871615
500 SC in grapes 2016 2017 CropScience
Limited
117 Study the efficacy of PlantbiotiX product 'Bacillus 01-04- 30-11- Zytex Biotech 526252
Subtilis ZB87-1/2 MTCC 5930' against powdery 2016 2017 Pvt. Ltd.
mildew diseases (Uncinula necator) in grapes
fungicide Juniper in different crops (Groundnut, paddy 2016 2016
and chilli)
(second season) Limited
120 Study of bio-products for quality evaluation of 01-07- 30-06- Isagro (Asia) 115000
pesticide residues and inorganic contents. 2016 2017 Agrochemicals
Pvt. Ltd.
121 Evaluation of Ca Essence on grapes 01-10- 31-03- Vanita 1372928
2016 2018 Agrochem (I)
Pvt. Ltd.
122 To study the effect of Drakshatar on growth, yield and 01-04- 31-03- Austenitic 263831
physiochemical parameters in Thompson Seedless 2016 2017 Steels Private
grapes Limited

no. From To (Rs.)
123 To evaluate the residues & persistence of FF1501 01-08- 30-07- FMC India 952200
(Valifenalate + Mancozeb) in grapes 2016 2017 Private
Limited
124 To evaluate bio-efficacy of Bacillus subtilis based bio- 01-10- 30-11- Novozymes 762026
fungicide, Taegro in grapes for the control of powdery 2016 2017 South Asia
mildew Pvt. Ltd.
125 To evaluate bio-efficacy of Bacillus subtilis based bio- 01-10- 30-11- Novozymes 760485
fungicide, Taegro in grapes for the control of downy 2016 2017 South Asia
mildew Pvt. Ltd.
126 To evaluate the bio-efficacy of Pydiflumetofen 7.5% + 01-10- 30-11- Syngenta India 587893
Difenoconazole 12.5% w/v (200 SC) against powdery 2016 2017 Limited
mildew and anthracnose disease in grapes after fruit
pruning
127 Analysis of residues of Glyphosate IPA salt 54% SL 01-06- 31-05- Monsanto 1495000
and its metabolite Amino methyl phophonic acid 2016 2017 India Limited
(AMPA) in cotton (cotton seed, lint, oil and cake) and
soil and determination of persistence / half life in
cotton plant (leaf)
128 To evaluate bio-efficacy and standardization of dose 01-10- 30-11- Sumitomo 760485
of Ethaboxam 40% SC in grapes against downy 2016 2017 Chemical India
mildew after fruit pruning Pvt. Ltd.
129 To evaluate the bio-efficacy and phytotoxicity of 01-10- 30-11- ADAMA India 760485
Captan 50% against downy mildew of grapes 2016 2017 Pvt. Ltd.
130 Study of Lattu (Soil Supplements) for quality 01-08- 31-07- Sumitomo 161000
evaluation of pesticides and chemical fertilisers 2016 2017 Chemical India
Pvt. Ltd.
131 To evaluate the bio-efficacy and phytotoxicity of oil 01-10- 30-11- R.G. Industries 276611
ORCHOL-13 against powdery mildew diseases and 2016 2017
effect on natural enemies in grapes
132 To evaluate bio-efficacy and phytotoxicity of Nativo 01-10- 30-11- Bayer 587893
(Tebuconazole 50% + Trifloxystrobin 25% WG) 2016 2017 CropScience
against powdery mildew diseases in grapes Limited
133 To evaluate the bio-efficacy of Cyazofamid 34.5% SC 01-10- 30-11- Insecticides 760485
in grapes for the control of downy mildew 2016 2017 (India) Limited
134 To evaluate bio-efficacy and phytotoxicity of 01-10- 30-11- Bayer 587893
Tebuconazole 430 SC against powdery mildew 2016 2017 CropScience
diseases in grapes Limited
135 To evaluate the residues and persistence of five 01-09- 31-12- BASF India 2938250
fungicides (Fluxapyroxad + Pyroclostrobin, 2016 2017 Limited
Metrafenone, Fluxapyroxad + Difenoconazole,
Boscalid + Pyraoclostrobin, Dimethomorph +
Metiram) in grapes.
136 Evaluation of Ca metalosate on grapes 01-10- 31-05- Indofil 662918
2016 2017 Industries
Limited

no. From To (Rs.)
137 To evaluate bio-efficacy and phytotoxicity of BAS 01-10- 30-11- BASF India 587893
750 02 F against powdery mildew of grapes 2016 2017 Limited
138 To evaluate bio-efficacy and phytotoxicity of Polyram 01-10- 30-11- BASF India 760485
against downy mildew and anthracnose of grapes after 2016 2017 Limited
fruit pruning
139 Evaluation of Paraquat dichloride 24% SL on grape 01-09- 31-08- ADAMA India 598000
against weed complex 2016 2017 Pvt. Ltd.
140 To evaluate bio-efficacy of 'ProPhite' against downy 01-04- 30-11- Isha Agro 398350
mildew, powdery mildew and anthracnose of grapes 2016 2017 India
after fruit pruning
141 Evaluation of PII 405 15% EC (tolfenpyrad) against 01-10- 31-12- PI Industries 962406
thrips and leafhopper in grapes 2016 2017 Ltd.
Divya Amrut-Soil conditioner on growth, yield and 2016 2017 Bioscience
quality of grape.
144 Evaluation of trials on physiological disorders 01-11- 31-10- Privi Pharma 501400
(Sunburn and berry cracking) in grapes. - A second 2016 2017 Pvt. Ltd.
year trial
145 To evaluate the residues and persistence of Admire 70 01-10- 30-09- Bayer 556600
WG (Imidacloprid 70 WG) on grape 2016 2017 CropScience
Limited
146 To evaluate the residues and persistance of Indaziflam 01-10- 30-09- Bayer 1032700
500 SC on grape and soil 2016 2017 CropScience
Limited
147 To evaluate the residues and persistence of Conika 01-11- 31-10- Dhanuka 929200
50% WP (Kasugamycin 5% + copper oxychloride 2016 2017 Agritech Ltd.
45% WP) in grape
148 To evaluate the residues and persistence of 01-10- 30-09- Dhanuka 504850
Cyflufenamid 5% EW in grape 2016 2017 Agritech Ltd.
149 To evaluate the residues and persistence of 01-10- 30-09- Dhanuka 504850
Amisulbrom 20% SC in grape 2016 2017 Agritech Ltd.
150 To evaluate bio-efficacy and phytotoxicity of IKF-309 01-10- 30-11- ISK 341331
180SC against powdery mildew of grapes 2016 2017 Biosciences
India Pvt. Ltd.
151 To evaluate the residues and persistence of Movento 01-08- 31-07- Bayer 1032700
Energy 240SC (Spirotetramat 120 + Imidacloprid 120 2016 2017 CropScience
- 240 SC) on grape Limited
152 To evaluate the residues and persistence of Movento 01-10- 30-09- Bayer 1032700
150OD (Spirotetramat 150OD) on grape and soil. 2016 2017 CropScience
Limited
153 Effect of LalVigne Mature on quality coloured table 01-01- 31-08- Zytex Biotech 573604
grape varieties 2017 2017 Pvt. Ltd.

no. From To (Rs.)
154 Residue study of azoxystrobin 11% + tebuconazole 21-01- 20-01- ADAMA India 622150
18.3% SC on grape 2017 2018 Pvt. Ltd.
155 Studies on the residues of Ferterra 0.4G 01-11- 31-08- E.I. DuPont 2205700
(Chlorantraniliprole) in grapes and soil season 2016- 2016 2017 India Pvt. Ltd.
17
156 Studies on the residues of Proquinazid 20 EC (w/v) in 01-10- 31-12- E.I. DuPont 2420750
grapes - season 2016-17 2016 2017 India Pvt. Ltd.
157 Effect of MEGAFOL on mitigating moisture stress on 01-09- 31-05- Valagro S.p.A. 799124
grape 2017 2018
158 Field evaluation of Tag Orbit against powdery mildew 01-10- 30-04- Tropical 376005
disease in grapes 2017 2018 Agrosystems
(I) Pvt. Ltd.
159 To evaluate the bio-efficacy of Arocyte against 01-10- 30-04- Farmex Agro 218052
powdery mildew disease of grapes 2017 2018 science
160 Effect of Prorise Package on productivity of grape. 01-04- 30-06- Nagarjuna 1144710
2017 2018 Fertilizers and
Chemicals
Limited
161 To study the bio-efficacy and phytotoxicity of 01-10- 30-11- Coromandel 585337
Difenoconazole 25% EC against anthracnose and 2016 2017 Agrico Pvt.
powdery mildew diseases in grapes Ltd.
162 Evaluation of crop protection molecules for residue 01-04- 31-01- BASF India 115000
analysis 2017 2018 Limited
163 To evaluate the bio-efficacy of Bio-Dewcon WP tris 01-10- 31-03- T. Stanes and 603228
Ampelomyces against powdery mildew disease of 2017 2018 Company
grapes Limited
164 To evaluate the bio-efficacy of Zuki against downy 01-10- 30-11- Vimax Crop 383709
mildew and anthracnose disease of grapes. 2017 2018 Science Ltd.
165 To evaluate the bio-efficacy and phytotoxicity of 01-10- 30-04- FMC India 986316
FF1702 against downy mildew disease of grapes 2017 2018 Private
Limited
166 To evaluate the bio-efficacy of Benalaxyl M 4% + 01-10- 30-11- Isagro (Asia) 361304
Mancozeb 65% WP (Fantic-M) against downy mildew 2017 2018 Agrochemicals
disease of grapes for one season. Pvt. Ltd.
167 To evaluate the bio-efficacy of Iminoctadine tris 01-10- 30-04- Sudarshan 691139
40%WP against powdery mildew and anthracnose 2017 2018 Chemical
disease of grapes Industries Ltd.
168 To evaluate the bio-efficacy of Oxathiapiprolin 3% + 01-10- 30-04- Syngenta India 709169
Mandipropamid 25% w/v (280 SC) against downy 2017 2018 Limited
mildew disease in grapes after fruit pruning
169 To evaluate the bio-efficacy of Pydiflumetofen 7.5% + 01-10- 30-04- Syngenta India 587893
Difenoconazole 12.5% w/v (200 SC) against powdery 2017 2018 Limited
mildew and anthracnose disease, in grapes after fruit
pruning.

no. From To (Rs.)
170 Effect of Zinatra and CaZBo in grape 01-09- 31-08- FMC India 854167
2017 2018 Private
Limited
171 To evaluate bio-efficacy and phytotoxicity of IKF-309 01-10- 30-11- ISK 350236
180 SC against powdery mildew of grapes. 2017 2018 Biosciences
India Pvt. Ltd.
172 Bio-efficacy and phytotoxicity studies on Indaziflame 01-08- 31-07- Bayer 938585
500 SC in grapes 2017 2018 CropScience
Limited
173 Evaluation of persistence and harvest residue of 01-07- 31-12- Atul Ltd. 1752300
herbicide, ALH 516 in/on sugarcane, soil and water 2017 2018
174 To evaluate the efficacy of three formulations of 01-09- 30-11- Evolva Biotech 1117908
Nootkatone and Resveratrol and compounds per se in 2017 2018 Private
control of downy mildew, powdery mildew and Limited
anthracnose of grapes as well as to study their effects
on shelf life of berries.
175 To evaluate the bio-efficacy of FF1703 against 01-10- 30-04- FMC India 286989
powdery mildew disease of grapes. 2017 2018 Private
Limited
177 To evaluate the bioefficacy of GPF 616 on downy 01-10- 30-04- UPL Limited 709169
mildew and anthracnose of grape crop 2017 2018
178 Evaluation of botanical alternatives Dr. Sure and 01-10- 31-12- Krishna Valley 757413
Amrut Miteout against thrips and mites in grapes 2017 2018 Agrotech LLP
179 Field evaluation of Kaligreen (potassium bicarbonate 01-10- 30-11- Tropical 421907
82%) against powdery mildew disease in grapes. 2017 2018 Agrosystems
(I) Pvt. Ltd.
180 To evaluate bio-efficacy and phytotoxicity of BAS 01-10- 30-04- BASF India 598339
750 02 F against powdery mildew of grapes 2017 2018 Limited
181 To evaluate the residues and persistence of Indaziflam 01-08- 31-12- Bayer 1059640
500 SC on grape and soil - season 2017-18 2017 2018 CropScience
Limited
Bupirimate 25% EC against powdery mildew of 2017 2018 Pvt. Ltd.
grapes.
183 To evaluate the bio-efficacy of NF-171 10% SC 01-10- 30-11- Biostadt India 709169
formulation on downy mildew of grape crop. 2017 2018 Limited
184 To evaluate the residues and persistence of 01-10- 30-11- Syngenta India 1354640
Pydiflumetofen 7.5% + Difenoconazole 12.5% w/v 2017 2018 Limited
(200 SC) and Oxathiapiprolin 3% + Mandipropamid
25% w/v (280 SC) in grapes.
185 To evaluate the residues and persistence of BAS 450 01-10- 30-04- BASF India 1276760
01 (insecticide) and BAS 750 01 F (fungicide) on 2017 2019 Limited
grapes.

no. From To (Rs.)
186 To evaluate spray positioning of Fluopyram 200 + 01-10- 30-11- Bayer 657012
Tebuconazole 200 - 400 SC (w/v) in combination with 2017 2018 CropScience
Trifloxystrobin 25 + Tebuconazole 50-75 WG and Limited
biologics against powdery mildew in grapes.
187 To evaluate the residues and persistence of 01-02- 31-01- BASF India 638380
Dimetomorph + Pyraclostrobin 18.7% WG on grapes. 2017 2018 Limited
188 To evaluate the bio-efficacy and phytotoxicity of new 01-10- 30-11- Indofil 788228
coded fungicide IIF-1316 against the downy mildew 2017 2018 Industries
on grapes. Limited
189 Effect of LalVigne Mature on quality of coloured table 01-12- 31-08- Zytex Biotech 503713
grape varieties. 2017 2018 Pvt. Ltd.
190 To evaluate the residues and persistence of 01-11- 31-12- Insecticides 571120
Cyazofamid 34.5% SC in grapes. 2017 2018 (India) Limited
191 Evaluation of dissipation and residue of fungicide 01-10- 30-04- UPL Limited 1753480
GPF 616 (Metalaxyl-M 3.9% + Mancozeb 64% WG) 2017 2018
in grapes.
192 Analysis of Benevia 10% w/w OD (Cyantraniliprole 11-10- 30-09- E.I. DuPont 265500
10% w/w OD) residues in pomegranate 2017 2018 India Pvt. Ltd.
fungicide Juniper in groundnut, paddy, chilli and 2017 2018
insecticide flonicamid in mango.
194 Evaluation of the bio-efficacy of Stimplex in grapes Acadian 479080
Seaplants
Limited
195 To evaluate the residues and persistence of 01-03- 30-07- ADAMA India 2219580
Fluensulfone 2% w/w GR (NIMITZ) on pomegranate. 2018 2019 Pvt. Ltd.
196 To evaluate the residues and persistence of Buprimate 21-02- 30-07- ADAMA India 2157040
25% w/v EC on grape. 2018 2019 Pvt. Ltd.
197 Developing need based precision technologies for 01-09- Mahindra & 607706
viticulture 2017 Mahindra Ltd
(M&M)


Annexure 15
Details of training programmes conducted for farmers and stakeholders
Sl. Programme title Duration No. of
No. participants
1. Recent advances in viticulture 18/06/2013 to 8
19/06/2013
2. Advanced techniques in viticulture 21/06/2013 to 25
30/06/2013
13/07/2013
4. Plant protection in two pruning and single cropping 07/08/2013 to 40
system in viticulture 08/08/2013
6. Mycotoxin residue analysis in various commodities 16/09/2013 to 44
17/09/2013
7. Plant protection and nutrient management in two 23/09/2013 to 15
pruning and single cropping system in viticulture 24/09/2013
8. Multi-residue method for pesticide residue analysis 30/09/2013 to 8
04/10/2013
9. Orientation programme on viticulture for 28/10/2013 to 6
horticulture extension officers from Mizoram 30/10/2013
10. A hand on training program on Pollen Cryo 27/11/2013 to 10
preservation 28/11/2013
11. Pesticide residue analysis using GC-MS in various 11/12/2013 40
commodities
12. Sampling of grapes from field for pesticide residue 21/12/ 2013 9
analysis as per RMP for grapes
13. A one day training cum awareness program on 05/03/2014 75
Protection of Plant Varieties and Farmers’ Rights
Act – 2001’
14. Production of quality grapes through good 11/03/2014 to 33
viticultural practices for peninsular India 13/03/2014
15. Sampling of peanut and peanut products for 23/04/2014 7
aflatoxin analysis
16. Screening of contaminant residues in food 20/05/2014 to 7
commodities 21/05/2014
17. Advanced Techniques in Viticulture 24/06/2014 to 28
01/07/2014
14/07/2014
24/07/2014
04/08/2014
21. Pesticide residue analysis in food using GC-MS/MS 04/08/2014 to 9
and LC-MS/MS 06/08/2014
22. Plant Protection in Two Pruning and Single 04/09/2014 to 30
Cropping System in Viticulture 05/09/2014
23. Training programme for growers of Champhai 10/09/2014 50
district of Mizoram under TSP

No. participants
25. Postharvest sampling of grapes for residue analysis 14/01/2015 49
26. Pesticide residues and plant growth regulators 02/02/2015 to 15
analysis using GC-MS/MS and LC-MS/MS 04/02/2015
27. Development of capacities for the provision of PTs 16/02/2015 to 29
under EU-India Capacity Building Initiative for 20/02/2015
Trade Development in India (CITD)
28. Enhancement of productivity and quality in grapes 10/03/2015 to 34
through hi-tech management practices 12/03/2015
29. Detection of pesticide residues through single 30/03/2015 to 29
residue methods 03/04/2015
30/06/2015
10/07/2015
32. Proficiency testing for agrochemical residues in 16/07/2015 20
Okra
33. Production technology for improving grapevine 22/09/2015 to 15
productivity 23/09/2015
34. Proficiency testing on aflatoxin residues in 05/10/2015 20
groundnut
35. Residue analysis of pesticides and plant growth 23/11/2015 to 19
regulators in fruits and vegetables using GC-MS/MS 25/11/2015
and LC-MS/MS
36. A technology transfer training programme on 26/11/2015 to 7
‘multi-residue method for pesticide residue analysis 28/11/2015
in spices by GC-MS/MS and LC-MS/MS
grapes
grape
40. Pre-harvest sampling of grapes for agrochemicals 08/01/2016 138
residue analysis
41. Training programme and proficiency testing for 07/04/2016 11
Aflatoxin analysis in peanut
27/06/2016
43. Integrated pest management in grapes 05/08/2016 to 25
06/08/2016
44. Training programme on pesticide residue analysis 09/08/2016 10
by LC-MS/MS (Xevo TQS) with special emphasis
on single residue analysis
45. Comprehensive Orientation Training in Grapes 07/11/2016 to 19
08/11/2016
46. Training and proficiency testing programme for 11/11/2016 15
pesticide residue analysis in grape
47. Training and technical advice on the preparation of 16/11/2016 to 16
ISO IEC 17043 accreditation 19/11/2016
48. Training-cum-Proficiency Test programs on okra, 17/01/2017 29
curry leaves and pomegranate

No. participants
49. Analysis of pesticide residue, antibiotics and plant 20/02/2017 to 25
growth regulators in fruits and vegetables 24/02/2017
50. “Pesticide residues and plant growth regulators 02/02/2017 to 15
analysis using GC-MS/MS and LC-MS/MS 04/02/2017
51. Training programme on analysis of mycotoxins 24/04/2017 to 15
residues in food 28/04/2017
29/04/2017
53. Trainer’s-training - program on ‘Analysis of 01/05/2017 to 20
pesticide residues’ 05/05/2017
55. Enhancing Grape Production with Advanced 27/06/2017 to 30
Viticulture Techniques 04/07/2017
56. Pre-post harvest sampling of fruits, vegetables and 29/06/2017 25
groundnut for pesticide residue / aflatoxin analysis.
57. Approaches for quality grape production after 26/07/2017 to 19
foundation pruning 28/07/2017
58. Advanced Approaches for Quality Grape Production 03/08/2017 to 33
05/08/2017
59. Integrated disease management in grapes 16/09/2017 24
60. Analysis of pesticide residue and plant growth 04/10/2017 to 28
regulators in fruits and vegetables 07/10/2017
61. Good agricultural practices for production of quality 23/10/2017 to 25
grapes 24/10/2017
62. Proficiency testing programme for pesticide residue 03/11/2017 to 15
analysis in grapes 03/12/2017
63. Training on single residue methods under the EU- 20/11/2017 to -
India Capacity Building Initiative for Trade 24/11/2017
Development in India (CITD)
64. Pre-harvest sampling of grapes for agrochemical 18/01/2018 to 23
residue analysis 19/01/2018
65. Pre-harvest sampling of grapes for agrochemical 20/01/2018 216
residue analysis


ICAR-National Research Centre for Grapes
P.B. No.3, Manjari Farm P.O., Solapur Road, Pune – 412307, Maharashtra
Tel.: 020-26956000, Fax: 020-26956099, email: director.nrcg@icar.gov.in
Website: https://nrcgrapes.icar.gov.in/

QRT CCRI, Nagpur

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ICAR-National Research Centre for Grapes, Pune

Quinquennial Review Team

Report of the QRT for ICAR-NRCG, Pune (2013-2018) 1

2 Report of the QRT for ICAR-NRCG, Pune (2013-2018)

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Genetic Resources Production Plant Health Food Comp. OLIC

Breeding Hort. I Pl. Pathology NRL

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1. Padma Shree Dr. K.L. Chadha, Ex DDG (Hort.), ICAR. : Chairman

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Production (000 MT)

Fig. 1. Grape area and production during last seventeen years.

2012-13 2013-14 2014-15 2015-16 2016-17 2017-18

Report of the QRT for ICAR-NRCG, Pune (2013-2018) 9

Value (Rs. crores)

Fig. 2. Scenario of grape export

5000 Quantity (MT) 12000

Fig. 3. Scenario of grape import during recent years

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3.1.4 Review of research progress

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