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12 Quadratic Least Squares Regression Calib PDF
12 Quadratic Least Squares Regression Calib PDF
Regression
also referred to as
Non-Linear or Second Order Regression
Quadratic Least Square Regression
Ref:
http://www.itl.nist.gov/div898/handbook/index.htm
NIST 4.1.4.2
Non-Linear Curve
Historically, many analytical methods have
relied on linear models of the calibration
relationship, where the instrument
response is directly proportional to the
amount of a target compound.
y = ax2 + bx + c
Where:
S(xx) = (Sxi2) - [(Sxi)2/n]
S(xy) = (Sxiyi) - [(Sxi)*(Syi)/n]
S(xx2) = (Sxi3) - [(Sxi)*(Sxi2)/n]
S(x2y) = (Sxi2yi) - [(Sxi2)*(Syi)/n]
S(x2x2) = (Sxi4) - [(Sxi2)2/n]
Statistical Equations:
External Standard
Cx = (-b { √(b2-[4a(c-Ax)])}) / (2a)
or
Internal Standard
Cx = (-b { √(b2-[4a(c-Ax/Ais)])}) / (2a * Cis)
Advantage
Ref:
http://www.itl.nist.gov/div898/handbook/index.htm
Section 4.1.4.2
Disadvantages
• Disadvantages include a strong
sensitivity to outliers. The presence of
one or two outliers in the data can
seriously affect the results of a nonlinear
analysis.