PROJECT REPORT

Antimicrobial activity of secondary metabolites produced from wild and mutant strain of
Bacillus subtilis against Plant Pathogens

Submitted for the partial fulfilment of the requirement for the award of the degree

Of

Bachelor in technology

In

BIOTECHNOLOGY (Molecular & cellular engineering)

Submitted by:

Ashutosh s. Masih

(10BIOTMCE007)

(2010-2014)

JACOB SCHOOL OF BIOTECHNOLOGY AND BIO ENGINEERING

SAM HIGGINBOTTOM INSTITUTE OF AGRICULTURE, TECHNOLOGY AND SCIENCES

(DEEMED-TO-BE-UNIVERSITY) ALLAHABAD-211007)
 CERTIFICATE OF ORIGINAL WORK

This is to certify that the study conducted by Ashutosh s. Masih (10BIOTMCE007) student of
B.Tech Biotechnology (Molecular & Cellular Engineering) VIIIth semester Department of
Molecular & Cellular Engineering ,college of Jacob School of Biotechnology and
Bioengineering, Sam Higginbottom Institute Of Agriculture ,Technology and Sciences,
Allahabad during January-June 2014 as reported in the present report was under my guidance
and supervision. The results reported by him is genuine and candidates himself has written the
script of the report entitled “Antimicrobial activity of secondary metabolites produced from
wild and mutant strain of Bacillus.subtilis against Plant Pathogens” is therefore being
forwarded for the acceptance for the partial fulfillment of the requirements for the award of the
degree of Bachelor of Technology in Biotechnology Sam Higginbottom Institute Of Agriculture
,Technology and Sciences ,Allahabad (U.P)

Place: Allahabad
Date: Dr. Rohit Lall
Asst. Professor
Department of Molecular & Cellular Engineering
Jacob School of Biotechnology and Bioengineering
SHIATS
 CERTIFICATE OF ACCEPTANCE

Certificate that the Project entitled “Antimicrobial activity of secondary metabolites produced
from wild and mutant strain of Bacillus subtilis against Plant Pathogens ”submitted Jacob
School of Biotechnology and Bioengineering, Sam Higginbottom Institute Of Agriculture,
Technology and Sciences, Allahabad in partial fulfillment of requirements for the award of
degree of Bachelor of Technology, Biotechnology (Mollecullar & Celluar Engineering) by
Ashutosh s. Masih, (10BIOTMCE007) VIIIth semester have been evaluated by us and found
satisfactory it is therefore recommended for the award of degree Bachelor of technology in
Biotechnology (Molecular & Celluar Engineering).

Dr.Rohit Lall
Asst. Professor
Department of Molecular & Cellular Engineering
Jacob School of Biotechnology and Bioengineering
SHIATS

Prof. (Dr.) B.P.Masih

Dean, JSBB, SHIATS

Date:

Chairperson

Project Evaluation Committee

 SELF-ATTESTATION

This is to certify that I, Ashutosh s. Masih (10BIOTMCE007) work entitled “Antimicrobial

activity of secondary metabolites produced from wild and mutant strain of Bacillus subtilis
against Plant Pathogens” has been personally done by me under the guidance of Dr. Rohit
Lall, teaching assistant, Department of Molecular & Cellular Engineering.

PLACE: Allahabad ASHUTOSH. S. MASIH

DATE: (10BIOTMCE007)