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    Chem

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
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    10 views22 pages

    Chapter 11

    Uploaded by

    Rochie Diez
    Chem

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    of 22
    ‘Mea ions ane Les als cg let pale: from elector donating Kes that re Lewis bases Cyside (CN eld 1 monedentat Izod teams binds = metal "rush ol ae tr (he ce oem Most wnson sea ons bd st Band atom DNigand int athe 10 a meal thug mor thn oe ganda sd em ‘entate ™muny otha, lating ligand (proncuneed nate "Asie chat gud is 2-daminosnne H.NCH,CH,NH,, als called ety amine) whom binding ts met fon shown ne mee. We that tyne ‘sdetails. "Tae chelate effet Ir te sity of melden Hand, to Tors more sable etl compere than hore formed by smar monodentate gas Fr example the ection of (CaHLO}” with wo melee eyleneamine s more arable han Ws econ with Foe molecules of mechani: & c Nn corto + any WH = +810 tdi a B kepeaxie ol oe a Hew, HCH, cmap renee | ee reininie | wow” | Saunca, on psx 1 pit 12 in the prsence of? M eyenelamiae and 4 M methylamine, te quotient [Calethyenediine*ViCAethylamig)" 820 ‘an import eden fen adenosine hosp (ATP), which binds dv lent talon Gach ae Mp" Mn", Co", andN") ough ow hx coednaton| ‘ston (Fgure I1-2) Te than sh postions ae cxcpied by wate moles. Te Stony sete form of ATP general the Mg comple ‘eal-heite sompletc ae sigs in bol: Bacteria sch x Eukeihi col and Salmonlaenterca Your su exccte a power on chelator elle ero Fue 11-3) scavenge ton tha esentl fr bacterial growth, The on-neohacn complex Ssrecppied a specie stes othe cel ell sara and taken i the ee Ho thes released ise th bacterium by enya susembly ofthe chlor To Fg bacterial ‘ction, your mane Syste rues» pin ced sterol Sequetr a tate erterobactn” An important mal eppiaion of hla} dseribed in Bo I+ 11 Meta-chelate Complenes FIGURE 11-1 eoDtoms eng 1:1 ‘pees it nt te, ng ‘ough rong a to agen as. ‘he stecoodnate ome of OTR iene empourd NNER 240 was ‘ete tom yeep en Uy'din tense cnn ange Camgonn dy me o OMT ‘Harmg mm 9 aa Stopes or aa) (egg con Comin emendane mason sans Kn} to doco in BOLE? Wehae dm ton amen a he cabal compe oth 0 ands t Sepeste pts) but ce somes ae a esse (ath 0 yds ace wea en. ‘Oxygen scared by te ie contin pin sep, which const of two pas of bunk dogpmte ad Batson mora gectic dese in wich te Pb of be ‘nolobin ents adit antes Chie itd ‘dh his ee sare oly wh reget isin fm Yo ‘oo el. The pola wir ree tha tots an Ise 48g oon pr yur rte hemline el ‘Thetody no mechani forcing requis oo, od escent frm the onic efi tm ne “To tune ion exertion, nme cheltion therapy wed ‘he meat succes dg seferioanine Bw perl Fe" thelr proce by the mcrae Sreponnce pT om on constant forthe et) ole, cle eosin Bs 0" ‘Used n conjunction with ascorbic yamine agent treet Fe" tte moe soll Fe" -deexamine cer several grams of fon per year fom an oveoaded pen. The Feroamie complex ieee he ne, ‘Deseronamine ries thn et er deen talsemi pent In pans or whom desert cece ‘only rot eed, ea 91% at of ea deme Fe so ViaaterIyeus of erp Thre ae megane els of eforon- mune tenet forex, igh dose tn chi owt, ° - ° eo 2, Fo eS °* 100 . fe ibe Som? eg Fe — ° of ov Lhe bonne Se go ° ° eo? cS o ° tis ee pc ctr ) Sythe ton comple enous Band yt cea ld capound inane in ch hed hs cc sete Gh ‘rns ot tenon ad on ph ean eye Desericnamie i expe and st etn hy overnight subeaancas finn ve leven igs or eck If shed "heh einen Many pte inn sos ave Ren etd Sod a efferive an that can be taken oly, hat fw hae need Clinical pactce™ The only aiministerd cheltordeferprone, ‘nrodaced in 967, rand with prs eft ean 50 coun ‘es (bat sna cesed nthe Unio Sats a Cana) Combined tse of desferioramine and dferiponeIncesesstrvival nad ‘edges th icidene aera seas, comparison with eer. oxamine teament only. An orly administered chelator ead {elersox wa approned forte te United Sates in 208 The ‘osinoed seach fr ew celts dices that 0 cure etme isfy effective tn he fog term, bone aro warplane ‘therapy might ce he dacs, eae Be FIGURE 1-2 6) Sec fearing phe (i) at ind tore haan tobe) Posse sch oa wal TP ep he et Ms oer CP ‘Sette bods hg « Amina aids in Figure 1-4 are syhtc coating agents Amin Nts snd a cartorglte Osos te pote lige tors nts oleces Figures 1-S nd 11). ‘Wee these msec ado amt on he ian atom ei proton A med pi th ofthe Kd DTPA in Fig 1-4 ied byte gly bond complex G” DTPA ‘ich nec io haan a conention of 0 mM provide cote mis “tion bse on comple formation i cle a complenomeretratn Lipale ‘ihe han NTA in Figure [14 fom song! completes wih al etalon xcept nia len one such as Li" Na, and K. The soto rgaes of the charge om J thee FIGURE 1-3 tentetestach compl Coton es me ain ce fe a sow toun hte dnd SSlephrer sed by mister ape tonto brusd by he eek Fon sos ces Enna sete tre om Goce taal Poon on ei Loon oe coy Leow wt sitet etna ti Sa aa ae A on IAS a woe Nei we NS vente tna FIGURE 1-4 snare of aay wd tn apes Nr 0 ted em 2 Cindeeta copie th etl ns eae ser om ope co a no FFICURE 11-5 sien FQ) inst FIGURE 11- smc of (PR fad inh st OTP) 240. The NaF The gd atthe Fe evncaote pra grams cornea cue tn am taupe Ons done Nom The er des fered 3 frelon) a lO == Manse nal 0 fond gi et 1 yn tan et ere Uncrate TePe stan seen cocina Fm Os, goo aga F-0 ns Oe ubanpupolbelen snarese, Pon oom Sets FAOB)| SMO Ate mC hme APionn WL aen A de nat Oa ue yang oe cs a Sper teste Copsey Cn a @ 20 [EDTA iby far, the mon widely wed cel in any chemistry. By dest iran or through an nde sguence of echoes, vital vey leben of he pede able can be ‘pesrd with EDTA ‘Acid-Base Properties EDTA is uherapac stem, designate HY" The highligh, ii hydrogen tm re the ones tat ae fos ypon meta sompes formation nogen, LCCO, nk, = 001C038) pk, = 2690.1) Bienen se PR = 15 (COs) p= 630M") HOCH, NCRCOH pk = 200(COsK p= 107 NI") Esa de 81M SSH ‘Theft fur pt vals apply 10 crboy! protons an he ato ae Fr the ance rotons, The neural ai is trap, withthe foal HeY. LY can be died at 10°C for 2b and wed as a rinary standard Iean be ds ved by dng NsOH slut tom a plastic enainer NaOH sluon om ls ote shu at te imedbecane i contains sllalineeuth mt leche fom the gla. Reage-rade [Nas¥ + 2,0 contains 0. excess wat Ir ay be sed In thi om wih sue cox. reton forthe mn af excess wae rd th composition NiHY »2H;0 a1 30. ‘he erthedrernce materiel CaCO, cn be we Yo wands EDTA o 1 vey the “The fraction of EDTA in each ofits protonated form: tpt in Figure 11-7 Asin ‘Sewion9.5,me cin define a foreach pee athe facton of EDTA in a oem. Feil ye i elit ae Fraction of EDTA in the form | fn (HAPTER TT OTA Trios ther [EDTA sth ttl concentration fle EDTA spies in the Slaton, By "ie ‘wemein EDTA ot corple meta ns Flow te derationnSston 99, wean ‘Bow tay isgtenby BRK Pe 7 he D IR) UEP, + TK + (EPIC, + TIP SRK + DR RA KAKA Table 1 gives aes orty+ a netion of AL as What does aye- mean? ‘The Fao of a he EDTA i the form Yi lle ays At pl 6.00 and form concen of 0.10 M, he eomposion of an EDTA olston = (HY =89 107M LY] = 89% 10M HY) = 28x 0M (HT =28>00°M hy I= 005M UN") = aM Dee Lato Find ay ‘Solution ay isthe faction inthe form Y ee avy MYT STAT = TAY) TT YE YS 18x10 © 9X10) FHI IO) + BAXIO) FABIO) > OR (008) (1.8% 10-5) sx 10% est Yourself A whit pH does ays = 007 Aner: = 1037) EDTA Complexes ‘The equlilvinm consant forthe reaction of ntl vith isn led he formation sonsant, othe sabiity constant ev Fomationconsm: Mey met gm yy ae "Nowe hat & for EDTA ified in terms the species" rating wth the mean Tae cuir coset cont ave hom des! fray the hers a of EDTA tne ‘oluion Eaton 11-5 should not Be interpreted vo mean tht only Yas with etd ons Table 1-2shows tat mato const formost EDTA complexes and ed ‘obe larger ormom pose chug cons 4 many tanston meal complexes, EDTA engulfs the metal on, forming the ss- sort pecs in igre 11 you yt bul pacefilig mal ox coordi ‘met-EDTA comple. Sou wil nd iin nthe chelate ns. Thr nine ehicved hen the O lipid are deawnback toward the Nats Such divon pear op aserenth oor 10 ¢M_— Se Bae ey iin Th pot sre a 2M SN Seneca cc eee come See tS fet orl Fade" nO OA agtEm neers ees {ou ca fm hcp ats EDTA completo es slater pcan be se hi ra ri. fors tesion maton of fine, tm go-Mreonpon (ap 3898) whch Mraneby OW weet ee ‘eum at gute cat eget Sas am we Senate omer mets Ply cc atte equ ont Fig 10 shows tow gH aft he tin Mandate sen eta ff Ca” wih EDTA. Below it~ the end poi x a sap eno lw scurne EFA 30a" nt Fe cian Th ta amnion ot eC" al fo op resco tow EEE] £014 Titration curves eh eta tation const be In tisection, we caluate he concentration of tee M" daring i tion with EDTA" fad pt tte son MO FETA MY R= aye K aun EA sarge, we eas conde the reaction o Be compte atch pit in the rin "Tettation carve erp of pM (= log) eas the volume of ded EDTA, “The coves analogous to ploting DH versus volume of tirntinan aed-bex tron, Tse ee naturel rego ofthe ton curve Fy 1111 [Region 1: Before the Equivalence Point In this pia, ei tees M* eft in soto fer the EDTA hes boca cose. The concentration of ree mt oni equal othe conemtatin of exes, nated Mr" The issocaton of MY" "is esi Region 2: At the Equivalence Point ‘Tre is excly a mach EDTA. gl nthe oto, We an eth lton 8 ith ‘been made by dislvngpure MY" "Some ee Mis poate by thigh dso ony" FIGURE 11-11 tee gre n EA Myr = ae + EDTA uso wino0 MESA socnne In this rata, EDTA epeeats fre EDTA inal is frm, A th quivalence pot, ffs is Team (r= (ECTA) everest ton pcr 1 EDTA Tinton Canes sete quence po hr nes inane pi, pra ‘Gr econo wih sate Sours ots 3 ond A [ED reo he total carcanentn of al eh gic pit isl al oe ‘lone af .DD. smal aout of on Co {ne gai wl ae OTR |" Region 3: after the Equivalence Point Now tere is excess EDTA, and viral all he metal on i inthe frm MY" The ‘oncentaton of fe EDTA canbe eguted tthe oneninton of exces EDTA ned aie tequelence pin, ‘Thration calculations Les eau the shape of the tration curve for he ection af 0.0 mf 0.0800 Ca?* (utc opt 10.0) with 0.0800 EDTA: Ca + EDTA -» Ca¥” = ay Ks = (030910) = 184% 19° ecto i are it resonable say thatthe retton gos to campeon wih each io of rant We wat makes graph which Cx (~ lela) plate era lites of ded EDTA. The equivalence volone 25.0 mb. revo 1: oe the euler bu rhierslrtynpricienolereenttepeue Tey See een a eee ee 1c (28529) eowor (2) a = Fon ‘Ogi! Dian top tre = 0029 1 Mop p> = “tes = 154 In sinilar manna me cood elite pC fr my au of EDTA ls tha 260 mL. Region 2: At the Equivalence Point away ll he mel sn he fem Ca¥". With nee dsc, te concen of (Ca¥ sequal te ongindconrueaon of Cu" wihacorecoa fo alsin al conenaton Final concentration) tay = tO go. 10! [ce HEDTA) - DSTA En 13,10" = LAK 1M, C2" = “gl a") = “lop = 5.85 Region 3: After the Equivalence Point In ths pon, ily al the me is inthe form Ca¥", al hr ees, neat EDTA. The coaentaton of Ca¥" and exe EDTA we cl eat Frexanph 2OmL thre 10 mL of exes EDTA. Coast. sama ~awovn (22) 190 aia ae ‘CHAPTER OTA Taine Pa soi tee = on001 ($8) 26 ‘The conenrtan of Ca" = 1a, x 10 ic yEDTAL 1263107) [e108 107 ea") = 19% 10M pea = an ‘Te sme st of caleation can be wed fray ome pt the uals pin. = 1.10" The Titration curve Caleulted tron curs for Ca sd Sia Fee 1-12 stow a sinc beak at he auyalece prs, where he spe ate, The Ca end ols more die than Sr endpoin beau the contol fomaion const ay" Kefor Ca¥" eeace on that of S*Y". The pH: lowered, he codon formation costa decrees Cause tay dere) andthe end point bosomes ese iin st we sawn ure 1-10 Te pt ‘tnt be used wily gh, because yan mga petite HIEEY © 0 1 with» spreadsheet tee Lats tor rates DA eeriaPane 24 edomeptin HOM ea eee Scupiaouctuciewon Meacatcionetsoncicas cen sceer ROMMEL mentale ‘tay ig ea eel tereeenreal Coartetatactann me aeeateaeeS Ga mms = wn sic fio nme he mai Ge my may Mobo MLK; ‘Tee mat lanes for mela gad are > pm (MIL) CLS el et erent Mas bn tan + oy = ste & Mas tae for tus iy = SH Sebsiing MILI (om Egon 1 fr Mine mat ses yes wo + Kuby = om a1) tue + Kp ann Now ste herpes fr fom Egon 110k to Eatin 1.9 ( av ati) Gate WN TER)” Ya snk de shou ef leo we rt sion aon vty — ME Spraticreoatinfor yy. _1* RIM TNE tn ‘aon of wih co TM= RINE be byKitL =O yn + BA As nace traonsin TH 10, he ration othe way oe ues on, ‘When 6 = 1, Vos Ya When @= 5, ¥,~ 1 Andon, ote wth a Spreadhost | FIGURE 1-15 spends or he ton fora pt ace hese paces ‘staat Seton 138 ws ‘Sand cnr od wma 50 2560 {nd dnco mad fh pce scion ‘er yt te CaS pe 5) 2M (ent ele eal 9 25060 eps by HTL EDTA a6 a ee [eee [sno s0mt of 64H CaF win 0s META e.2 [ae — [oe iis =| 308] testa] ones | ears me as] 44te-05| — Too | 250000 or] suerio | Foo | TmEar| — soot] as018 a [soot 5 | bs = -SASIO CHOCO 6 [revsasion(oroF rss 0/950) WW laaorusessanes [| For afitation with EDTA, ou ca follow he drain trough nd id that he oma sion contr shouldbe replced in Eqution byte condtional omation cn. [Ranch aplicn ate fae pt of he tation, Fie 11-13 shows = spreads which Eguaton ITT] feed to calculi the Co raga cue in Figure 1112. AB in ace tira, yo inp inclu B eM = logs] andthe opt inclu Ei ome ‘tan To fin te i pol ary pM wt Y chse w 0. Ifyou even the procs dt lgnd with ctl onthe ation othe wa tthe esac point the iene ofthe ation in Eaton 11-1 vena MLE KUM Spratt conten fry Gate_SUNY a ‘ation of GT gn = ry Complexing Agents EDTA tiation conditions nhs caper wre selected te prevent meal oxide pei: tin ate ehsen pH To permit ny metalstoBe ated in alin solos with EDTA, ‘te ose analy complexing nent. Tis regent + gan, och mmo, te, ‘Sate oethanolamig tht ins the mee steely enough pent mal hydroxide ‘rom precpating fot weiklyeoauphf ive op the mal when EDTA is added. Za” is ‘hl tvted in ammonia fer, wich Hohe pH and complete metal ono kept isola. Les ce bow ths wor. Metal-igand Equilibria” Conia mean hat forms wo compet wih he aur complexing Hip: m7 Mote = ' bem wae any ts) Me=M, Be an 7 Fe teal ome ‘The eva contin, ya cle oeral or cumulative formation constants The {raion of met on i th competed state, M can be expressed aus) here Mish ol concentration ofl fos of M(* M, ML, and ML nts 8) "Now ts in etl exes or ayy The ans tlsce For met Mug (MI [ML] + Ma} earner 14 EO tains ~ Equation 1-19 and 1-14 allow us sy {ML} = BML) an (M3) = BML Theeloe, Ma = (81+ BAM) + BANE = (on + BAL = BLE Subunit esa ft Egon 11-15 ives the dese et + BIL) pa ihe mel ferns morta a cmp, Eston Sethe far ~Vepuitie pate Pron of a) eee n Int + Billa > BALE par ‘Ammonia Complexes of Zinc “aan Ny or sonpenen ZH", ZH", ZN" ZN Ite cogcenation of fe, mpreouatd NH 0.10, id he Hatin of sn a he form Za (ay pH ther wil ssa be some NH in equi wt NH) Solution Agent pe famaton estat for tecomplens ZatNH?* (B= 102%, Zui Ge EZ = 10" an I B= 1), Te appropriate frm of Equton 1-16 1+ Bil) + BaLr + BIL) + BLT uation 11-7 gives the ration fin fhe form Za", atin [L] = 0.10 Mand he foe vals ope y= = 18% 10% which meas the very ieee 2a the presence of O10 Ni, Test Yoursell Find a fe, nprotnsted [NH] = 002 M. Answer: 20072) any on Hydrolysis Decrea ston 11-8 tes tht he effect (Codons) maton cone stint for an EDTA comple the prod ofthe oration ont, tes he tation meta inthe fr Mest tion oF [EDTA inthe fom Y=? = yg cys Ky Table 1-1 olde xia i Hum it Teves a) mer pl 1 In Secon 11-3, we ha 0 autlaycomlenng Lipa aad we ‘mpi ested tat aye ~ Ia at nt onset wth tert form MOH), species. Combination ofp al el nin ‘Section 11-3 vere elected so ht yea 0 MUO), ne ‘ie Wecan fed sch condos for mot Mons, bt fr Mi lor M. Eenin aii solution, Fe" hyo t0 (0H) and OH." (appendix ves formation constant or yond comple.) Tie ap shows hat ay ele oI bance pH tnd 20g og. 0), but thn dope hydro care tp 5, the fraction oF eID in he oem ef 10 ‘The efectne fomaton conta for FY" inte grok tee comrtutons ‘Asp increase: ay eres, so KM inten ApH increases, ‘mal hye occurs Saye deren The cen in ay faecal by te decreas in es s0 Kf oly ota ore HD. The thir conbation I" Bay which ee faction of ‘the EDTA complet inthe form FY” Aw py sone of th ca plex alas pstonto orm FAHY, which decemev eye pt 1 Aun Complesog Agents Inthe pl ace 2105, ays ly const tn neta ant lms solution, compleer sich x FAHY" an (ROH YI sae fomed and ag doreane. Tie-home mess ahs bok, we rest uve sax Inch thre ic mo hyo and yes tale by elie tly added auxiliary Hand In ely byes of Mand MY inences most EDTA trations and mes he thetic moe complied thin we pretend nhs hp, otra td oth ci lomaon ent, “sre Cares we ademas esas AH ICH eRe FeO FCO Fe aa ——rrrnrrrrrereoroertrer'rverere | Aish tive fan conta at fed pnd ed cana of any ‘Snot et fon 2 deeb the ens mea ys on he ‘ei oon nc. ‘he on 2h = er Co felons ‘ram eatin 1-5 EDTA Titration with an Auiliary Complexing Agent ‘Now cone ion a Zn” by EDTA i the psec of NH, The extension of Egan 1 reutes ow ona omaton ont scout fo he ft stony see of be TH te oY an lyme ten nx bound tO EDTA isin the em 2 Kr = epost: Ki auin, Ins expreion ani en by Equation 1-1 apd ays given by Equation 1-4 For teu ale pl and Ne can compte Kad proceed with ration clelsions {is Sv 11-3, ebtiaing K for Kj An sumption i hs process shat EDTA i teh songs complexing get un anon ro een lly allEDTA binds Za” ul the ‘men ion conse, EDTA Titration inthe Presence of Ammonia ‘Conse be raion f 50.0 ml of 1.00 10°" M Za wit 00 10° M EDTA a IO; he reece of O.10 MN Tis i the concentration of NH Tse abo [Nisin be solution) The oqvaeace pot isa 500 mind pZn* afer won of 200, 500, a 00 mL of EDTA, Solution In Equation 1-17, e found ha ays = 18 x 10%. Table Hee hat oer = 20, With i fom Table 1-2, he condom fomaton cont r= ngage y= (18% 10 "030,10 = 1.7 10" (a poi equator it. ml Bec he suns eit 00 ma te outan of 2°" reining 8 3.0400, The dition factor S070. Theo, he ones of ie no bound EDTA i a = (28) 0( 82) 4030 oo ala nt bd EDTA ond wy Te sin pen = ~bog{Zn"")] = 8.11 Lee aay et Tepe FHP "9H = ‘which does noc exceed the solubility product of Za(OH), (Ky ~ 10'S"). 0) descents prt nl eas a be in eg ‘eusantes ar ns Si oe senator Coos SEDTA = Za¥" Ini concenraion() 00 S010 inl concenration (Mex S00 10 tx (v1 S00 «10% ONT INS EDTA] e Ee Cope SAM (a1 ay Coss = (LX IOS & 107) = 9.7% 10"? = pea" = ~loe(2n'"} = 12.01 (6) Ar the equivalence po't—60.0 ml: Alot l zn isin he form 20°. With “dao itr of 301100 fe =e we od (ov 500.) 190 x 10"*M) = 65 x 10" (228) anox10°¥9 =45310 ‘We ao Ino the concvaton fees EDTA, whose dion facto is 10001100: leptay (88) nop 9a COMPTER 1 ETA Trains Once we how (ZaY""Jand EDTA, we an we the eyalitiom constant Fad Za": 16 sy Ke = R= (02010) = 95 x 10° 7 iss x 10-4) * 95 x 1 (aay = 99% 10 iar yo x10) rae = 1528 Aft the equine point th roblen doesnot depen onthe presence of NH cate ‘we know bah (24Y" Tan [EDTA Cos Test Yourself Find yZn'* aerating 00and SLO mL ofEDTA. Anown8.35,1428) J Figure 1-1 compuresthe exealtdttation cus fo Za inthe pene of dite fen ‘nt concentrations of suxiltury complexing agent. The erate the concentration af NH, the dea ‘aller te change of pZa"" ea he equlencs point. The slay ian mast be kp on ee oe wT telow the eel hat would obser the end pit of he ation. Calor Ph shows he ‘eas rat appearance of «Cu -amsraiasolation Ste an EDTA tiation Racin ata eae EEG etal ton indicators Se ‘The most commen vy to det the oi in EDTA tion ic witha metal ion indie tor Alot nce amereury elect Fue 1-10 and Eerle 14B) an ano ‘cleave dec (Sesion 14-6) A pH eect wil llow the coun ofthe ton m4 PON etn mae bute lun, ene HY" esis 2H when forms met comptes i as ‘Metalion idan Tbe 1) ae smponds tht change cler wheat into 3 MEH eto ‘talon. Ufa irs mst ind etl ss ston thn EDTA ce papery ‘ipa aos Intra by he tan of Mae" ws HBA ot pt 10 with Calmagt indenter Moin +EDTA + MEEDTA + tn a Ace santo the eget a al soe finds) add the colores soon ‘of Mg" to fora ed complex Ar EDTA Is ade. cat fint wth es eos Me™ ‘When fee Mg" & sed up the lat EDTA a before the eqiedene put dsplaces index trom he ed Md complex. The shuns or there Ml tote uabund Io signal te end plat ofthe tration Dement 11. 19) The ctor mart mae Rao FTA DEMONSTRATION 11-1 Metal fon Indicator Color Chan ‘This demoosaion vars the color chat aos with dye whove coor alles the appearance ofthe slion before and Reaction I-19 and sows how asoond dye canbe aed to po> afer heaton, Adding 3m. of methyl ve Tale 10-9) (oe dace amex ely dated co chance, many ther yellow des) rods: an rane clo io othe std point and a gren color ater Tis sequence of ealor i bow Srocx Samos Fn Color Plate Butler (pt 100) Add 142 mo consort (14.5 M) ‘queer ammonia to 17-5 of ammo crise and 19 one ‘te 9250 m with water. aft MeChs: 0.05 M i 06 EDTA: O08 MN HLEDTA- 2440 qa Pepa asoluion containing 25 mL of Mets. Sm fut, * oa and 300 mL of es Aad eps of Eescteome nck Te a ; CCalnsgit nator Cae 113) and trate wih EDTA. Note the a oso ‘ola chage om wie ed pal ue tthe em pot (Color Pate ‘eng om fa). The specrncon change accompanying the color change > ye Cant nd te tay apn araia sown ie ere Dees fone Eat Hat sa Ehren one For some obrerers the change of indestor color ic not as Saray farsi uence ae sary ws dese, The oles can be alected by nding anne? SABLE 11-5 common metal jon Indicators Santee Caer eal a vm. ‘oe Snes vale wa pees Een on faim 0% oO wis ten We ary Yager i= 1s Hie £ re e 9 NH ‘Yellow (with, rue olf S-4 £NMo sa Mi, tte SOT iN, Nit 109 Hsin violet Ge) rd °° eur tke oo ° on moe te 0c co TRE iv Xyleral BN oni, = 670 delle mee oe Boyce; ama, ome scion it Te ey aan ‘ violet om on ° on peor int Pyrocatechol Pk = 78 Hla yellow he volt SO pK, = 98 ‘Hala® viotet sory See ‘inn 00 ve dmb pra ed ‘nc ed Facey ty Sau nn 25a i ee fee cera owe {DumleaSp0ce a te as Mow meta on indore rao sis incon, wih pi als ited a Table 113 Becsine the color af fe indestor pT dependent, mot indatrs ca used ony in cer fuinpH anges For example yenl orange (pronounced eel chage om yellow © ‘el hc bids o atl aya pH 55. Thi color change iesy to observe. At pH 7.5, {he change is from lt ooo and fel ee A specropotomeer an meas he hor change, torts more convenient f we can nei Bue 11-15 sows pH anges in ‘thick many meta can be tr snd ndestrthat aewefl ia diferent races. "The nditor mst ie up 18 metal on the EDTA metal dos no fly sock seroma nage edo ac the ior rear bck Tra N™- Cot Co", Fe™ and ADI came be wed forthe de tration ofa imac cng seaman offs eta rc be wed fr ack tn, however For eample, es standard ears pte Ca Then intr al le os BTA ces eiaeesn reteset wih Me, |= ‘cup 1 EDTA Tratons FIGURE 1-15 Gudea Eo ato {onmon mas Up enor ou pant ‘ch acon ithe ua Ba ‘ole sows peg chat Conley ae eua pve met tom pecbising Calman veal, tho reeme big #9) a be ‘ted fr EB cast an Case 065 0.0 Aint 16 sec’ qren aca ate { omepepl [tino eT Ce Gyrmce ad rye be eset ie ‘case Poon 8 ends pe Pic rihanna! Re oct corpleone Pa Pete Pe outed vt “Pett omeone Yo votumie bis 9 bse 10, ange me Singh, 1adianbense fonpergsget aay ‘SSoaonea| Soto enar can | ‘sone “Then anita or EOTA tate Fatih ates ttre Cranes trom be pa yl EDTA Titration Techniques ‘Bec so many lnetscan be aye wih EDTA, thr etn Here ding thay arts ofthe rcs Direct Titration {na rect tration, ral: irted wth saved EDTA. Anas heed pl ot ch he conditions formation conta othe maa EDTA copies an the ke ofthe fe inate tat ferent rom ht of he retahi Sompee 11 OTA Tation Tecnu a1 Ditcintor onmartedcgoe” fies twats grea ys Sipe poe tava ore eb Shem ce nan rperenehion ‘maby aang tomtom ve fet, Ucn pede Reason corsets ah te ‘Byes ns here Secon ih ued ced sthseenting tn eyo The rol. ‘hte foosrtties med o bserdes ‘Stowe spodven _s8thedinasctn a £08 -Ausitiarycompleing agents soch ss NH, tate, cre, or wthanolamie may be upp sven nt fom presi i the seen of EDTA. Fer example PO" ieted in Kistler at pl 1 in tbe presence of reat, wich complexes Pan does tot alow POOR), 0 precip The Teadarae compen rust be les sable tah te Tent EDTA comple of heaton would not be esi ack Titration. Ina back ttratlon, known excess of EDTA i aed to the analyte Exceas EDTA Hanae precipitate inthe sbuece of EDTA, i rete too slowly with EDTA, Fi ‘Mocks th indector The meal on forthe back tration ast at dislace analyte fom EDTA. |Back ttrtion TNE cane analysed by wba raion wis sandr 2a 3 with lena orange Fades, A slo consining 2500 of No in it HCI weed wi 25.00 mL oF {Cosa #3 MNs-EDTA, The olin nestled with NOH, andthe ps adjusted 0 {5 with sein ter Th soon tre yelow when dapsone aor abl. ‘ionon with 00299 M 21°" regres 71m to ech the ed end po. Wh ste sant of NI inthe waknows? Soliton The unknown was ttl with 25 00 ml of 0052 83M EDTA, whi coins (2500 ma N0.082 83 M) = 13208 mal of EDTA. Back trated (17.6 i) (00022999) = 04049 mmol of Za" Because I mol of EDTA ress with 1 ol fay rectal io, hee must ave een 1.3208 mmol EDTA ~ 04049 mmol Za ~ 09159 mol NF “he concstton OF 0915 9 25.0 mL ~ 0.63654 M. ‘ost Yourself back aon requir 1300 a 2a", what wasthe egal woes tron of Ni"? Answers 040 38M) ‘ack tittion prevents precptaton of analyte Fe sample, KOH) presi t ‘eed to pt 7-8 with sod sett, nd Boel to emare comple fomation of sb feltble AIEDTA) The clon then cold: Camas idea is ad; ad back ‘ation vith standard Zs prtened Displacement Titration "or not hive asa ist, ba lacerent ration feb Hie treated with exces MEUEDTA) to spac Me", whichis rated with standard EDTA. Me Mey? MYT ara “Te coltion fermion const for He(EDTAF™ must be greater thn Kor MgEDTAY. tse Me wil a be diplced rom M(EDTA) "Tere is no sutible dco for Ags However, Ag™ wll space Ni ea tae yaoncilated om Ange 4 NUENIE + 2AR(CN; + NE “The ited Ni cn thn bed with EDTA ff ut how much AC” wo 2d Indirect Titration “Anos ht pcp wt cre meta an an ena vite CDTA by inet ran. for example, mite cin be analyzed by precipaion wth cass: Eu" at Hf 1. The us, washed apd thn ule with excess EDTA atpH 100 Bey Bu? Buk nto ‘olution as BEDTAY Excess EDTA i ack sated wit Me™ “C)MPTER 1. DTA Taine eines cette thle cmt Crp 2) hms, To eas fades temples eae wih oie ok wae ny Ca and Mi" wes Hues commonly (rhage) oes Fe so Fe and Coto at spree a heuer af liam of CeCO, pet ten wih qa omak F"Cus ands the mat me iter Ths (CS) + Mp] eM, we wold y atch om Toon ay EDTA at 10 ip NA, afore oes tes 00m CACO, per ierbeceme 100 me CaCO, 1 rmol tol coment ns of Ca" and Mg’ Carta be ding CiCO, Wate of anes em th me CACO, perience Spray itera incr ou ft 13 to ams Seed e's dae above 20 mute wat Arty pf, MOH, pcp and Ics 0 EDT? ‘mide beh terre by may sn an eed ye coke ard waters with yp 0 foo alban st nico pr reeeremE (ay lai tnt cer aol eros Ser tegen Ine gveshn Es .00{9) +C0:+ HOCKHCO an) — Enough soap ocomme Cx" and Me™ matte wed to so? Het comers etna it crore (vine off 003) is avubble for kning. Had water lees sold pis cle’ jeipans Ca20, sale taco ater ier The facto & ‘atone wenitoaponin bth watt oooh hrs de w CaCO) cal paar handn seni. Hares ett inion Water este ane fee i clusion Cy prepaono C200) op ay garth on ed 1 fovea eae ose) aii parce yeas omar aman dosonedeASD) eae insoiland eet nese the permesblty ofthesolto wet SO permanent hans Basal oe Oy hea ater icon concrete pte, and po - 4 Altematvly at aio ane precipita wthexcess tnd met on The psipitte Is eed and wae, and cess ine lea brated wth EDTA. Anions ah at CO} .C102", 5", and 801 can be detemined by indiet iaton with EDTA,” Masking ‘Amaskng agents eagent th pros some compart ofthe lye french Ming pvt ne specs om nrg EDTA, For example Al’ ina mine of Ms nd AP" cin be meauredby fea mang inthe tata of note Mags at ‘he A” with Ey herey eying ony the My" fo rss with EDTA, setrend0 £14 ron 60113 ges an {Cyanide sks CO" Za Hg", Co"".Cu", Ag". NF, PE PEF" ad fe", SRPOIMRaPPON of masking but not Me" Ca, Mn, of Po. Wie yielded 0 sion ons Cd nd Po caly Po" rats wth EDTA. (Caton: Cy orm oe Eons HCN blow III. Janie soutions shouldbe storly baie and ony fan n= od) Fede fake AI”, Fe’",T"sand Beato: HE for by Fin acd soit emenely etrdos and shuld ot cont hn and ey. my ot mest) pan tu ie fected are shoud be hooded with tran hen tated with calm ate pl that sa ‘yu ave on had befor the acideat it id rvigen mit wea er goves to ptet Hoar, o40458 Atemseven) Taedanlamine asks AD", Fey and Ma" 2 dire opal sae eeeat ‘masks B.C", Cu Hand PO Demasking ass eto roma macking gen Cyanide compe an bedamaked wet onal pH MCN + mH,CO + mitt + min + Me ‘Remline ‘ow “hiourea masks Cu" by reducing itt Cu" and comping the Co", Cope tn be Ue sted rom howe byoxiaon wih HO, Sly afferded by meine demssng and pH contol allows infivsulcompoocn af complet mcs of real onto be anja Dy EDTA tet, ‘Terms to Underst am ucla completing Wat compleomeic tation

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