Liceo de Cagayan University

High School Department

Senior High - Main Campus
RNP Blvd. Kauswagan Road, Cagayan de Oro City
S.Y 2019-2020

General Biology 1 Laboratory

A Narrative Report

(February 5, 2020)

Submitted by: NickoVicente

Crishin Mae Labuyo
Claire Maestrado
Shanthea Cabana
Ulysez Bart Jumao-as

Submitted to: Ms. Cindy Love Cagas

 Introduction

In this Narrative Report it will show about an activity of using a microscope in

a small organism and the observation in each activity we do.This activity was conducted
at the Laboratory last February, 5, 2020. A Microscope is an instrument used to see
objects that are too small to be seen by a naked eye.

 Discussion

Letter “e”

Procedure:
1.) Obtain a blank microscope slide,cover slip, a dropper, and a printed “e”.
2.) Orient the letter “e” on the slide so that the letter is in normal reading position.
3.) Add a drop or two of water and cover with cover slip.
4.) If air bubble are trapped, tap the cover slip gently.
5.) Focus this temporary mount under LPO and examine it .

Observation:
When every time you adjust the LPO the bigger will you see the letter “e” under
the microscope and the dark spot will get bigger also.

Documentation:
S LPO HPO
Skin Hair

Procedure:
1.) Place single strand of the finest hair on a slide.
2.) Add a drop of water and cover with cover slip in such a way as to trap a bubble air.
3.) Place your temporary mount under low power objective and examine it.

Observation:
As you adjust the lens the more you can see it closely. In this activity, it can be
seen the thickness of the hair strand under the microscope.

Documentation:
S LPO HPO

Cheek Cells

Procedure:
1.) One lab member needs a clean toothpick. Carefully rub the toothpick on the inside of
your cheek.
2.) Gently scrape the inner side of the cheek using a toothpick, which will collect some
cheek cells.
3.) Place the cells on the glass slide that has water on it. Mix the water and the cheek
cells using needle and spread them.
4.) Take a few drops of Methylene blue solution using dropper and add this to the
mixture on the slide.

Observation:
As the the lenses get higher the more you can see the cell in the microscope,
when the lens reach the HPO it is the most clear and you can see the crisp image already.

Documentation:
S LPO HPO

Onion Cell

Procedure:
1.) Obtain a small piece of onion skin from teacher and a cup of iodine,
2.) Place the onion skin on a clean slide and use one of the eye droppers to place a small
drop of iodine on the onion skin.Do not drench the onion skin, one drop is plenty.
3.) Carefully place the slide cover on the trying not to have any air bubbles under the
slide.
4.) View the slide under the microscope using low power objective. Work you way to the
higher views.

Observation:
The clear epidermal cells exist in a single layer in this activity.
Documentation:
S LPO HPO

Passing through the gate of cell

Procedure:
1.) Remove a small piece of fleshy scale leaf from a red-skinned onion bulb.
2.) Pull off the thin outer purplish epidermis from the piece of scale leaf/
3.) Mount it on a glass slide.Drop a small amount of water.
4.) cover with a cover slip and examine under the LPO and the HPO.
5.) Make a labelled drawing of an onion epidermal cell.
6.) Using a dropper, place 5 to 10 drops of 5% sodium chloride solution at the edge of
the cover slip and irrigate the salt solution across the slide by holding a piece of filter
paper at the opposite edge.
7.) Observe the cells under the microscope for several minutes and take note of any
changes.
8.) Make a labelled drawing of an epidermal cell after the treatment with the salt solution
with water.
9.) Using the same technique in step 6, replace the salt solution with water.
10.) Examine the slide and observe any changes for several minutes.

Observation:
The epidermis is separated and scattered in this activity.
Documentation: