RID: 4AFY5UMD114

Job Title:Protein Sequence

Program: BLASTP
Subject:RecName: Full=Alpha-amylase type A isozyme; AltName: Full=1,4-alpha-D-
glucan glucanohydrolase; AltName: Full=HvAMY1; AltName: Full=Low pI alpha-amylase;
Flags: Precursor [Hordeum vulgare] ID: P00693.1(amino acid) Length: 438
Query #1: Query ID: lcl|Query_10207 Length: 511

Sequences producing significant alignments:

Max Total
Query E Per.
Description Score Score
cover Value Ident Accession
RecName: Full=Alpha-amylase type A isozyme; AltName:... 37.4 85.9 27%
6e-07 24.71 P00693.1

Alignments:
>RecName: Full=Alpha-amylase type A isozyme; AltName: Full=1,4-alpha-D-glucan
glucanohydrolase; AltName: Full=HvAMY1; AltName: Full=Low pI alpha-amylase; Flags:
Precursor [Hordeum vulgare]
Sequence ID: P00693.1 Length: 438
>alpha-amylase type A, EC 3.2.1.1 [Hordeum vulgare]
Sequence ID: AAA32929.1 Length: 438
Range 1: 40 to 122

Score:37.4 bits(85), Expect:6e-07,

Method:Compositional matrix adjust.,
Identities:21/85(25%), Positives:38/85(44%), Gaps:3/85(3%)

Query 64 QGGDFQGIIDRLDYIKDMGFTSIWLTPVFDNQPKGYHGYWITDFYKPD-EHFGSMETFKK 122

GG + ++ ++D I G T +WL P + GY Y D +G+ K
Sbjct 40 SGGWYNMMMGKVDDIAAAGVTHVWLPP--PSHSVSNEGYMPGRLYDIDASKYGNAAELKS 97

Query 123 LVEEAHKRDMKVVLDFVVNHVSPEH 147

L+ H + ++ + D V+NH ++
Sbjct 98 LIGALHGKGVQAIADIVINHRCADY 122

Range 2: 309 to 348

Score:33.5 bits(75), Expect:9e-06,

Method:Compositional matrix adjust.,
Identities:16/44(36%), Positives:22/44(50%), Gaps:4/44(9%)

Query 305 TFIDNHDMERFTRLLVKENVFPGTRWKLALAYMYTTPGIPIVYY 348

TF+DNHD + FP + AY+ T PGIP ++Y
Sbjct 309 TFVDNHDTGSTQAMWP----FPSDKVMQGYAYILTHPGIPCIFY 348

Range 3: 27 to 39

Score:15.0 bits(27), Expect:6.0,

Method:Compositional matrix adjust.,
Identities:6/13(46%), Positives:8/13(61%), Gaps:0/13(0%)
Query 493 VLFLLFIYLVWKR 505
VLF F + WK+
Sbjct 27 VLFQGFNWESWKQ 39