43.1.34
Decant ether-fat solution into flask, containing porcelain
AOAC Official Method 950.54
Fat (Total) in Food Dressings
First Action 1950
Final Action
Thoroughly mix test sample, and accurately weigh ca 1 g, by
difference, into Mojonnier tube. Add 10 mL HCl, shake, set tube in
water bath heated to 70°C, and bring to bp. Boil 30 min, shaking tube
thoroughly every 5 min. Remove from water bath, add H2O to fill
lower bulb of tube (but not neck), and cool to room temperature.
To mix ture in Mojonnier tube add 25 mL ether and shake
vigorously ≥1 min. Add 25 mL petroleum ether and again shake
vigorously ≥1 min. To break emulsion centrifuge 5–10 min at ca
300 rpm.
chips or glass beads, that has been dried at 100°C, allowed to cool in
air to constant weight, and weighed against similar flask similarly
treated as counterpoise. Rinse off mouth of tube with small volume
ether after each decantation, letting ether run into flask. Repeat ether
extractions twice, using only 15 mL of each ether for second and
third extractions. Again shake vigorously after addition of each
ether and centrifuge. If necessary in order to decant all ether–fat
solution after first extraction, add more H2O prior to second
decantation.
Slowly evaporate combined ether solutions in flask, dry ca 90 min
at 100°C (placing counterpoise in oven at same time), cool in air to
constant weight, and weigh.
 2005 AOAC INTERNATIONAL