Microbiology 20: Grading Guidelines for Lab 33

Microbiology Unknown Identification

Los Angeles Mission College
Item Points Comments
Unknown Number -10 Penalty if it is missing or incorrect
Gram Reaction 10 Verify with instructor at beginning
Morphology 10 Verify with instructor at beginning
Arrangement 5 May have multiple arrangements
Sketch of Gram stain 5 Show color, shape, arrangement,
magnification
Motility 5 Hanging drop
Others staining results 5 Only necessary stains, capsule, endospore,
acid fast etc.
Colony diameter 2 Streak plate
Colony appearance 2 Streak plate
Other colony characteristics 2 Color, margin, etc.
Agar slant growth 2 Age, amount, pattern, color
Growth in broth 2 Age, amount, pellicle, flocculent, etc.
Tests to identify unknown 15 Perform tests from flowchart in lab manual;
will vary for each unknown.
Flowchart for your unknown 10 Construct flowchart on separate sheet.
Describe the process you followed in 1-2
and narrative
paragraphs, including any problems or
unexpected results encountered.
Species name 10 Identification of correct species
Confirmatory test(s) 5 Do at least one to two confirmatory tests not
in flowchart. Consult Bergey’s Manual.
Other characteristics 5 Any other relevant information
Questions 5 Answer all questions in back.
TOTAL 100 Other penalties: contamination, etc.