What is Plant Histology?

Plant histology is the branch of biology concerned with the composition and structure of plant tissues in
relation to their specialized functions. Its aim is to determine how tissues are organized at all structural
levels, from cells and intercellular substances to organs.It is also known as “botanical microtechnique.”
These methods find broad application in plant physiology,
development, pathology, and molecular biology.
Tissue processing...
The aim of tissue processing is to embed the tissue in a solid material firm enough to support the tissue
and give it sufficient rigidity to enable thin sections to be cut, and yet soft enough not to damage the
knife or tissue. In order to keep slide for long time, we should prepare permanent slide of plant tissues.
For permanent preparation, there are several steps and plant tissue must pass through all the steps. All
steps are depend on time and concentration of chemicals. Steps of Permanent slide preparation of plant
tissues: 1 - Fixation 5- Embedding 2 - Dehydration 6- Trimming 3- Clearing 7- sectioning 4- infiltration 8-
Staining

Staining botanical
tissue sections
is as much an art as it is a science.
The most common all
purpose
botanical stain is
Johansen’s Safranin and Fast Green stain.

Whole-mounts, where an entire organism or structure is small enough or thin enough to be placed
directly onto a microscope slide (e.g. a small unicellular or multicellular organism or a membrane that
can be stretched thinly on to a slide)
Dry mounts are the easiest microscope slides to make. You will need a glass slide and a cover slip. Dry
mounts work best for samples like pollen, hair, feathers, or even dust particles caught in a microfilm
filter.
The basic steps to prepare your own dry mount slide are very simple. Place the sample on the slide and
cover.