S A R C O D I N A

TROPHOZOITES Size Movement Pseudopods Chromatin Nucleus Cytoplasm Inclusions

Regular,fine, Ingested
Entamoeba histolytica Unidirectional, Finger-like/knife-like even Small, centrally Finely granular rbc,bacteria w/
(Amoebiasis) 12-35µm progressive distributed karyosome scanty vacuoles
chromatin
Thick, unevenly Coarse Bacteria w/
Entamoeba coli 12-20µm Non-motile, Blunt, granular distributed Large, eccentric granular, numerous
(Commencal amoeba) sluggish peripheral karyosome vacuolated vacuoles
chromatin
Entamoeba hartmanni Regular,fine,
(small race of Finger-like/knife-like even Small, centrally Fine granular
E.histolytica) 4-10µm distributed karyosome
chromatin
Large, central,
Endolimax nana 8-10µm Blunt, numerous absent irregular, granular Bacteria w/
(Commensal amoeba) sluggish “blot-like” scanty vacuoles
karyosome
Regular,fine,
Iodamoeba butschlii even Large, centrally Granular &
(Commensal amoeba) 10-15µm Blunt, finger-like distributed karyosome vacuolated
chromatin
pattern
Moderately Evenly Large,central Leukocyte,
Entamoeba gingivalis 8-15µm progressive blunt distributed karyosome epithelial cells,
granular rbc, bacteria
Dientamoeba fragilis Evenly Single w/
(Commensal amoeba) 6-15µm Non-motile Propeller-blade like distributed granulated bacteria
karyosome

だし
 CYSTS Size Chromatin Nucleus Cytoplasm Inclusions
Entamoeba histolytica Regular, fine, even Cigar shaped
(Amoebiasis) distributed Quadrinucleated Finely granular chromatoidal bars
(1-4) w/ rounded ends

12-15µm Needle shape

Entamoeba coli Thick, unevenly chromatoidal bars
(Commensal amoeba) distributed 5-8 nuclei Coarse granular w/ splintered ends,
diffuse glycogen
mass (appears red in
iodine
Entamoeba hartmanni Short
(small race of 5-10µm Fine & evenly 1-4 nuclei Fine granular grain/grape-like
E.histolytica) distributed chromatoidal bars
Endolimax nana Granular & No glycogen
(Commensal amoeba) No peripheral 1-4 blot-like vacuolated vacuoles, mostly
chromatin karyosome granules
8-10µm Coarsely granular & Large glycogen
Iodamoeba butschlii vacuolated, vacuole that stains
(Commensal amoeba) absent 1 only eccentric achromatic well w/ iodine
granules on 1 side
Entamoeba gingivalis
No cysts stage
Dientamoeba fragilis
(Commensal amoeba)

だし
F L A G E L L A T E S
Trophozoite Cysts
▪ Old man w/ eye glasses; bilaterally ▪ Ovoid
symmetrical ▪ 4 nuclei & median body
Giardia lamblia ▪ Pyriform or teardrop shape ▪ Central karyosome
(Gay bowel syndrome) ▪ 2 nuclei w/ large karyosome ▪ Cytoplasm retracted from cell wall
▪ 4pairs of flagella ▪ Interior flagellar structures
▪ 2 median bodies, 2 axonemes & 2 sucking
disk
▪ Falling leaf motility
▪ 10-15µm ▪ 6-8µm
▪ Pear shape ▪ 1 nucleus
▪ Stiff rotary motility ▪ Lemon shaped w/ clear hyaline knob
Chilomastix mesnili ▪ Flagella:
(non-pathogenic flagellates) 3 from anterior
1 from cytosome region
▪ Inclusions: prominent cytosome; spiral
groove

Trophozoite Habitat Size Nucleus Undulation membrane Inclusion bodies Specimen for
(Trochomonas) diagnosis
Trichomonas hominis Intestines Medium Ovoid As long as costa stool
Trichomonas tenax Oral Smallest Round 2/3 of the costa none Oral scrapings
cavity
Trichomonas vaginalis Less than half of the costa Siderophil Urine, vaginal
Genitals Largest Ovoid granules discharge, prostatic
secretions

だし
C I L I A T E S
Trophozoite Cysts
▪ Motility: rapid, non-directional ▪ 2 nuclei:
▪ 2 nuclei: Kidney shape macronucleus
Balantidium coli Kidney shape macronucleus Small spherical micronucleus
(Balantidiasis Small spherical micronucleus ▪ 1-2 contractile vacuoles
▪ 1-2 contractile vacuoles ▪ Double cyst wall
▪ Food vacuoles, bacteria ▪ Row of cilia between cyst wall layers
▪ Cytosome present
▪ Numerous bristles
H E T E R O K O N T
Trophozoite
▪ 5-10µm
Blastocystis hominis ▪ Shape: spherical to ameboid
(Blascytosis) ▪ Color: refractile but stained w/ iodine
▪ Inclusions: large central vacuole w/ peripherical nucleus &
cytoplasm

だし