Spot sample Length of bands (cm) Rf value

Methanol 10% 3.2 3.2

= 0.60
5.3

Methanol 20% 3.4 3.4

= 0.64
5.3

Methanol 30% 0 0
=0
5.3

Methanol 40% 0 0
=0
5.3

Methanol 50% 0 0
=0
5.3

Methanol 60% 0 0
=0
5.3

Methanol 70% 0 0
=0
5.3

Methanol 80% 3.9 3.9

= 0.74
5.3

Standard 4.2 4.2

= 0.79
5.3