Purpose Of Test \ To measure Blood sugar(in this test below for

.FBS)

:Equipments
Spectrophotometer 2- Test tubes no. 1 2 3 -1
Water bath 4- Syringe+ Needle -3
Tourniquet 6- Kit of blood sugar (standard + reagent) -5
Timer 8- Macro Centrifuge -7
Alcohol+ cotton -9
\Procedure
.Draw blood sample from person (normal or abnormal) -1
Put sample in test tube then separate it in centrifuge at -2
.3000RPM for 10 minute
Draw 10µ of sample serum and put it in test tube then add -3
.1000µ of working reagent to it (test sample tube)
.Add 1000µl of working reagent to test tube (blank tube) -4
Add 1000µl of working reagent to test tube that contain 10µl -5
.Of standard solution (standard solution tube)
Test (t.)or
Blank Standard(s.)
Sample

Working Reagent µl 1000 µl 1000 µl 1000

Standard - µl 10 -

-
Sample (serum) - µl 10
 →
Incubate tubes for 10 minute at 37c°. Then read optical -6
density
.At 505 nm ).O.D(
a- use blank tube for reset spectrophotometer -7
b- read and record Absorbance of Standard
c- read and record Absorbance of sample
\Normal Range
FBS=65-110 mg\dl
RBS=90-170 mg\dl
\Calculation
tA
sA
×sC =Glucose concentration
At= Absorbance of test (pink color)
As= Absorbance of Standard
Cs= Concentration of standard =100 mg\dl

653.0
001
×
4.0

0×01 =98gm ¿ 0.89

.From the result above, the person has normal FBS