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Eyedonation 140504024247 Phpapp02 PDF
Eyedonation 140504024247 Phpapp02 PDF
• IMPROVEMENT IN PRESERVATION
METHODOLOGY
• CORNEAL SUBSTITUTE
• UTILIZATION OF THE OTHER COMPONENTS
OF THE EYE
Other uses:
• Donated Sclera can be used for glaucoma ,
oculoplastic and retinal surgeries
• Human amniotic membrane can be used
for ocular surface procedures
• Fair and equitable distribution of
transplantable tissues to corneal surgeons
acco to waiting list.
STEPS OF EYE DONATION
1. Donor selection
2. Tissue retrival
3. Corneal examination
4. Tissue transportation
5. Storage of corneal tissue
6. Distribution
DONOR SELECTION
1) AGE OF DONOR:
no influence of age on tranplant
outcome.
Older age : usage rate declines
Lower limit : 2 yrs to prevent myopic shift
after keratoplasty
2) Medical history review
Eye banks must have consistent policies for
the examination and documentation of
donor's available
• medical records,
• medical history
• cause of death
• Medications
• laboratory reports
3) Legal consent taken from next of kin
enucleation
• OPTISOL :
storage period max up to 14 days
OPTISOL GS:
+ GENTAMICIN & STREPTOMYCIN
Normothermic storage system
• Organ culture
• Incubated at room temp in nutrient medium
• Storage perid : 30 days
• Glass bottle containing
1. 100 ml of MEM medium
2. Earle’s salt soln
3. 2.5% fetal bovine serum
5. L – glutamine
6. Antimicrobial agents
cultured bottle is closed & icubated in dry
and at 31 - 37▫ C
Uses of Donated Eyes
Corneal Transplantation
Emergency Patching
Medical Education
Research
EYE BANK ORGANIZATION
THREE TIER ORGANIZATION
An integrated
system involving a
three-tier community
eye banking pyramid
based on the
infrastructure and
manpower at all levels
.
• The three tiers
proposed were eye
donation centres, eye
bank and eye bank
training centres.
• . The top tier comprises
EBTCof 5 Eye banking
training centers (EBTC)
• responsible for
1. tissue harvestin processing &
distribution,
2. creating public awareness
3. training and skill up-gradation of eye
banking personnel.
Eye banks
• middle tier would comprise of a strong
network of 45 Eye Banks(EB)
• These would cater to a population of 20
million each. These Eye Banks would be
closely linked with 2,000 Eye Donation
Centers- EDC (ratio of 1: 50 suggested),
each of which would cater to a population
ranging from 50,000 to 100,000.
EYE BANK PERSONNEL
1) EYE BANK INCHARGE:
2) EYE BANK TECHNICIAN
3) CLERK – CUM– STOREKEEPER
4) MEDICAL SOCIAL WORKER /
PUBLIC RELATION OFFICER
5) DRIVER -CUM - PROJECTIONIST
• 1. General supplies
a. Donor information sheet, consent forms, etc
b. Pen-light—for gross examination of eyes
c. Insulated container with water ice and special foam to
transport the tissue
d. Supplies for blood collection
e. Non sterile preparatory gloves
f. Broad spectrum antibiotic solution
g. Eye protection (safety goggles), shoe covers
h. Disinfectant solution
i. Eye caps/prosthesis
j. Biohazard disposable bag
k. Gauze and cotton pads
Two small closed stainless containers for gauze pads
soaked in 70 percent alcohol in one and 5 percent
betadine in the other.
• 2. Autoclaved and sterile materials:
is Simple!
Removal of Eyes
takes 20 minutes only
Does not delay
funeral arrangements
Eye Balls
should be removed