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    304 No of Pages :2 Course Code: 088.501 Roll No: (To be filled in by the candidate) PSG COLLEGE OF TECHNOLOGY, COIMBATORE - 641 004 SEMESTER EXAMINATIONS, FEBRUARY / MARCH - 2015 BTech -BIQ TECHNOLOGY — Semester: ‘08B501 ENZYME ENGINEERING AND TECHNOLOGY Time: 3 Hours Maxtnmim Marks: 100. INSTRUCTION: Group I and Group IT questions should be answered in the Main Answer Book Answer aity 5 questions in Group IL Answer ALL questions in Group T and Group TIL Group I - Multiple Choice questions - (which will be given to the éandidates half an hour before the scheduled clase of the examination) should be answered only jn the jpace provided inthe Main Answer Book, GROUP T Marks: 10x 3=30 1, “List the six class@s of enzymes and briefly describe the role of each class 2. Detine “Total activity’ and ‘specific activity of an enzyme 3. Differentiate between competitive and non competitive enzyme inhibition with an example 4. Draw a Cleland plot for sequential and. ping pong bi substrate enzyme reactions 5. Mention the, important characteristics of solid matrices commonly Used for covalent binding of enzymes What is Damkohler number? State its significance. ‘Adcount on the use of immobilized enzymes in amino acid production What are enzyme electrades? Give an example Name the Sensing elements used in biosensor and their mode of selection of an analyie What are abzymes? Mention any one of its application eee SoexaD GROUP II Marks: 5 x 12 =60 11, Outline the different mechanism by which enzymes mediate catalysis 12, Givé-an overview of the various mathads of enzyme immobilization with their major advantages and disadvantages. How the immobilizing matrixes affect the enzyme reaction kinglics? 13, Describe in detail the “diferent steps. involved in isolation, purification. and characterization of industrially important intracellular enzymes. 14. Design a biosensor scheme for detection of glucose:giving clearly the reactions involved and the transducers used 15, Discuss the applications of enzymes in food and pharmaceutical industies. 16. Explain any two. method used for‘enzyme engineering along with their commercial applications JENDI FD/IU, Page No: | 304 No of Pages :2 Course Code: 088.501 Roll No: Write the Alphabet of your choice answer for each question in the space provided im the Main Answer Book {Do not attach this question paper along with the Main Answer Book) FEBRUARY / MARCH = 2015. O8B501 ENZYME ENGINEERING AND TECHNOLOGY GROUP II Marks: 10x 1 = 10 1) © Maximal velocity is obtained when A) Sisincreased B) Eis saturated with § GY pH is optimum 1D) Temperature is” optimum Il) > Bsezymes differ in the following properties except A) catalytic B) Kinetic G) electrophoretic mobility D) subseliular distribution Ui)... Zymogens are, A) Holoen zymes B) inactive proonzymes C) isozymes D) allosteric enzymes TV) if the Kq. of an ‘enzyme for its S increases while’ Vas. remains constant as the cencentration of inhibiter increase s, the mode of inhibition is A) Competitive 6) noncompatitive C)mixed D) no inhibition V) __ dmmobilized enzyme used in produstion ot HFCS A) Hexokinasé B) invertase C) Amylase” D) Glucosa Isomerase VI) For Industrial production of Glucoamylase , following organism is widely used A) Aspergillus niger B) Ganoderma lucida G) Saccharomyoas cerevisiae D) Salmonella typhi Vil) The enzymes used in amperometric biosensor are A) Oxidoreductase B) polymerases C) Hydrolases D) Ligases Vil)

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