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Japanese Dental Science Review (2014) 50, 69—77

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Tooth brushing for oral prophylaxis


Haruaki Hayasaki DDS, PhDa,*, Issei Saitoh DDS, PhDa,
Kuniko Nakakura-Ohshima DDS, PhDa, Mika Hanasaki DDSa,
Yukiko Nogami DHa, Tsutomu Nakajima DDSa,
Emi Inada DDS, PhDb, Tomonori Iwasaki DDS, PhDb,
Yoko Iwase DDS, PhDa, Tadashi Sawami DDS, PhDa,
Katsushige Kawasaki DDS, PhDa, Nozomi Murakami DDSa,
Tomoya Murakami DDSa, Mie Kurosawa DDSa,
Masami Kimi DDSa, Akiko Kagoshima DDSa,
Miki Soda DDSa, Youich Yamasaki DDS, PhDb

a
Division of Pediatric Dentistry, Department of Oral Health Science, Course of Oral Life Science,
Graduate School of Medical and Dental Science Niigata University, 2-5274, Gakkocho-dori, Chuo-ku,
Niigata 951-8514, Japan
b
Department of Pediatric Dentistry, Graduate School of Medical and Dental Science, Kaghosima University,
8-35-1, Sakuragaoka, Kagoshima 890-8544, Japan

Received 23 November 2013; received in revised form 8 April 2014; accepted 25 April 2014

KEYWORDS Summary Control of plaque and debris is essential for the prevention of inflammatory
Tooth brushing; periodontal diseases and dental caries, because plaque is the primary etiological factor in the
Oral prophylaxis; introduction and development of both of these infection-oriented diseases. Plaque removal with
Dental plaque; a toothbrush is the most frequently used method of oral hygiene. Powered toothbrushes were
Toothbrush developed beginning in the 1960s and are now widely used in developed countries. The bristles of
a toothbrush should be able to reach and clean efficiently most areas of the mouth, and recently
the design of both manual and powered toothbrushes has focused on the ability to reach and clean
interproximal tooth surfaces. An individual’s tooth brushing behavior, including force, duration,
motivation and motion, are also critical to tooth brushing efficacy. Dental floss and the type of
toothpaste play additional important roles as auxiliary tools for oral prophylaxis. Dental profes-
sionals should help their care-receivers’ meet the requirements of oral hygiene to maintain their
QOL. This article reviews these topics.
# 2014 Japanese Association for Dental Science. Published by Elsevier Ltd.
Open access under CC BY-NC-ND license.

* Corresponding author. Tel.: +81 25 227 2908; fax: +81 25 227 2908.
E-mail address: hayasaki@dent.niigata-u.ac.jp (H. Hayasakia).

http://dx.doi.org/10.1016/j.jdsr.2014.04.001
1882-7616/# 2014 Japanese Association for Dental Science. Published by Elsevier Ltd. Open access under CC BY-NC-ND license.
70 H. Hayasaki et al.

1. Introduction of microorganisms not individual pathogens) in order to


understand its biology and functional implications [7]. The
Oral prophylaxis is the foundation of oral health, and daily clinical presentation of these dental diseases is a net result of
plaque removal is considered important for oral health. the cross-talk between the pathogenic dental plaque biofilm
Specific oral bacteria, generically known as ‘‘dental plaque’’ and the host tissue response. In the healthy state, both
are the primary cause of gingivitis (gum disease) and caries. plaque biofilm and adjacent tissues maintain a delicate
The removal of dental plaque is thought to play a key role in balance, establishing a harmonious relationship between
the maintenance of oral health. There is some evidence that the two. However, changes occur during the disease process
electric toothbrushes, other than those with a counter-rotat- that transform this ‘healthy’ dental plaque into a ‘patho-
ing movement, are more effective than manual brushes for genic’ biofilm. Recent advances in molecular microbiology
tooth brushing [1]. One explanation might be the varying have improved our understanding of dental plaque biofilm,
dexterity of the participants in the different studies. Clear producing numerous clinical benefits.
deficiencies in manual tooth brushing have been recognized
both from epidemiological and clinical researches [2—5]. This 3. Toothbrushes
article reviews the contemporary literature to provide an
overview of present knowledge concerning tooth brushing. 3.1. Manual toothbrush design

2. Dental plaque During the 18th century, the bristle toothbrush came into
use. Forerunners of today’s brushes were developed in the
A great diversity of microorganisms–—over 700 species–—has 1930s. These nylon toothbrushes with plastic handles were
been detected in the oral cavity [6], and evidence shows that easy to manufacture and therefore more affordable, making
the investigation of specific microorganisms or associations of tooth brushing a common practice in Western society. Ever
microorganisms as etiological agents of periodontal diseases since, much imagination and inventiveness has been applied
and caries is a simplistic approach. Instead, dental plaque to toothbrush design, and now numerous models of manual
must be studied as a biofilm (i.e., as communities composed toothbrushes are available [8] (Fig. 1), with more than 450

Figure 1 Examples of typical bristle profiles [8].


Tooth brushing for oral prophylaxis 71

kinds in Japan. However, there is still insufficient evidence bristles during brushing. Once independent motion is
that any specific toothbrush design is superior to another. achieved, the longer bristles can effectively reach farther
Modern toothbrushes have various bristle tuft arrange- between the teeth [16].
ments (e.g., flat-trim, multilevel, angled) that are designed A recent systematic review [9] indicated that, depending
[9] to enhance plaque removal from hard-to-reach areas of on the plaque index used, a 7—9% improvement in efficacy
the dentition, particularly from interproximal areas. The (with the Navy index and Q&H index, respectively) can be
degree of hardness and stiffness of a toothbrush depends achieved with a multilevel bristle tuft configuration com-
on the filament characteristics such as its material, diameter pared to the traditional flat trim. The most recent develop-
and length. Today many manufacturers vary the length or ment of angled (‘‘angle bristles’’ in Fig. 1) rather than
diameter of the filaments mounted in a single head. Tooth- vertical bristle tuft arrangements appears to have made a
brushes with thinner filaments are softer while thicker fila- significant contribution to interproximal plaque removal.
ment diameters are stiffer and less flexible. The number of Clinical studies have consistently demonstrated that a brush
filaments per tuft also determines the hardness of a tooth- with an angled bristle tuft configuration is significantly more
brush, which in turn has an effect on the cleaning perfor- effective [8,17,18]. Slot’s review also showed that depending
mance. on the plaque index used, a 12—15% improvement in efficacy
Robertson and Wade [10] showed that subjects cleaned (with the Q&H index and Navy index, respectively) can be
significantly better with medium and hard brushes than with achieved with this particular bristle tuft configuration com-
a soft-bristled brush. Berdon et al. [11] found that a tooth- pared to a flat-trim design. Angulation appears to be an
brush with 0.18 mm diameter filaments was significantly less efficient innovation of brush head design, based on the
effective (P < 0.05) in cleaning than were five brushes with results of a review by Cronin et al. [19].
larger diameter filaments from the same manufacturer. Gib- Recently, Voelker et al. [20] compared various commercial
son and Wade [12] observed that a toothbrush with 0.2 mm manual toothbrush and powered heads to characterize the
diameter filaments tended to clean the marginal gingiva following: bristle size, shape, diameter, number of tufts,
more effectively than another with 0.18 mm diameter fila- number of bristles per tuft and surface characteristics. There
ments. In a crossover study, Vowles and Wade [13] tested the were significant differences for toothbrush bristle diameter
differences between 0.13 mm and 0.28 mm filament dia- and bristle shape. In contrast, there were no significant
meters and found that plaque removal was significantly differences between powered toothbrushes and manual
better (P < 0.001) with the thicker filaments when used with toothbrushes in bristle diameter, bristle count and tuft
the roll technique for brushing the facial and interproximal count. The results suggest that although there are wide
areas. It appears, therefore, that filaments must have a variations in toothbrush head designs, significant differences
degree of stiffness to dislodge plaque deposits. are found only in bristle diameter and shape.
Designs are based on the premise that the majority of
persons in any population use a simple horizontal brushing 3.2. Powered toothbrushes
action. Over time, the design of the brush head has evolved
and multiple tufts of bristles, sometimes angled in different Powered toothbrushes were first introduced commercially in
directions, are now used. Today, prospective users can read- the early 1960s [21,22] and have become established as an
ily find a toothbrush with a handle size appropriate to their alternative to manual methods of tooth brushing. As a rule,
hand size, and much emphasis has been placed on new the advantage of the powered brush is both clinical and
ergonomic designs [14]. Toothbrush manufacturers have statistical improvements in overall plaque scores. Powered
made great effort in considering many different aspects toothbrushes offer an individual the ability to brush the teeth
when designing new models to meet the challenge of enhan- in a way that is optimal in terms of removing plaque and
cing plaque biofilm removal through improved tooth brushing improving gingival health–—conferring good brushing techni-
efficacy. A few toothbrush manufacturers have also made the que on all who use them, irrespective of manual dexterity or
effort to evaluate tooth brushing efficacy. training [23]. Results showed that, powered brushes were
Product design changes can yield genuinely improved always better than manual brushes (Table 1).
performance characteristics [15]. A major shortcoming of There are two published Cochrane systematic reviews
conventional flat-trim toothbrushes has been a ‘blocking comparing the efficacy of powered toothbrushes and manual
effect’ of tight bristle tufts, preventing individual tufts from toothbrushes [5,24]. The first suggested that the rotation/
reaching interproximal areas. Multilevel toothbrushes have oscillation type of powered tooth brushing is superior to
been developed with alternating rows of longer and shorter manual tooth brushing for the removal of plaque and reduc-
bristle tufts acting independently, uninfluenced by adjacent tion of gum inflammation. However, that review did not allow

Table 1 Reported comparisons of plaque removal efficiency between powered had manual toothbrushes.

Authors Year Manual brush (MB) Powered brush (PB) No. of subjects Differences
MB < PB (%)
Nathoo et al. 2003 Oral-B Cross Action Colgate Motion 126 42.1
Singh et al. 2005 Oral-B Indicator Colgate MicroSonic 39 52.9
Ghassemi et al. 2013 Oral-B Indicator 30 CS Spin brush GLOBRUSH 103 12.8
MB: manual toothbrush, PB: powered toothbrush.
72 H. Hayasaki et al.

direct comparison between different types of powered Hence, movements of the toothbrush during different brush-
toothbrushes, due to the small numbers of trials using other ing techniques could be characterized.
types of powered brushes. Therefore, no definitive conclu- The most recent study developed a toothbrush-monitoring
sions can be drawn regarding the superiority of one type of system that provided information concerning the orientation
powered toothbrush over another. Only minor and transient and linear displacement of the toothbrush actions [33].
side effects were reported, and cost and reliability of the Orientation and back-and-forth displacement of the tooth-
brushes were not reported. Further trials of good quality will brush were measured by a combined three-axis acceler-
be required to establish if other types of powered tooth- ometer.
brushes are better at reducing plaque and gingivitis. These Tooth brushing behavior is a daily habit, therefore, it is not
findings were not advanced much in the second review. easily altered, even after professional instruction in the
Another systematic review for powered toothbrushes pro- clinic. First, teaching brushing technique is a complex and
posed the necessity of methodological homogeneity in future time-consuming procedure. Second, from the perspective of
studies in this field to enable quantitative comparison of movement sciences, skill training requires many repetitions
results [25]. of the same movements to incorporate them into an indivi-
dual’s habitual motor program.

4. Tooth brushing behavior


4.2. Toothbrush force and pressure
4.1. Techniques for manual toothbrushes
The choice of toothbrush is usually a matter of individual
To identify the most effective methods of tooth brushing in preference rather than a demonstrated superiority of any one
children, Muller-Bolla and Courson [26] carried out a sys- type [42]. The enthusiastic use of a toothbrush is, however,
tematic review to evaluate the children’s ability to remove not synonymous with a high standard of oral hygiene. Adults,
dental plaque. The horizontal technique was found to be the despite their apparent efforts, appear not to be as effective
most effective up to 6—7 years of age [27—29]. Advantages of in their plaque removal as might be expected. Most indivi-
the horizontal Scrub are that it is easy to learn and practice duals reduce plaque scores by approximately 50% during
and is effective at plaque removal [30,31]. However, this tooth brushing. A 1-min brushing exercise in participants
tooth brushing method is less effective for cleaning in the adhering to their customary brushing method, but all using
proximal and gingival sulci of permanent teeth and may the same type of toothbrush, observed a plaque score reduc-
results in gingival recession and tooth abrasion [32]. For tion of approximately 39% [42]. These results indicate that
older children, there was no statistical difference between most people are not effective brushers and probably live with
the Bass and Fones techniques. Bergstrom et al. suggested considerable amounts of plaque on their teeth, despite
that the horizontal technique should be advised in younger brushing at least once a day. What currently is lacking is a
children. For adults, the modified Bass technique is often systematic review that provides a reliable overview of tooth
recommended by dentists and in textbooks and used in brushing efficacy through the process of systematically locat-
clinical studies [33—35]. However, the Fones technique is ing, appraising and synthesizing evidence from individual
often recommended in patient brochures in Germany, and its trials [9].
efficiency recently was proved by Harnacke et al. [36]. In Professional recommendations for individual oral hygiene
general, these brochures, recommendations and instructions mostly include tooth brushing at least twice daily [43,44] for
of tooth brushing technique are very helpful for improving 2—3 min with gentle force [39] using the Bass technique or
oral hygiene. However, for serious improvement in a patient’s modifications of it [33,45] as suggested by American Dental
oral hygiene, the dentist should first evaluate the patient’s Association. However, ‘‘gentle force’’ is not defined clearly
hand-skill motion before giving instructions. Each individual enough to be used in the clinical situation.
has poor (or weak) or favorite (or strong) hand-skill motion When brushing force is increased, more plaque is removed
for each of the brushing techniques. From this point of view, [46]. Numerous studies have reported brushing forces. Some
toothbrushing technique is still an open question. of these previously reported tooth brushing forces were
As for the effectiveness of tooth brushing instruction along 2.95 N [47], 2.61 N [46], 2.96 N [48], 3.23. N [42], 2.3 N
with practicing a particular tooth brushing method, Slot et al. [39]. Force discrepancies might have been due to random
[9] summarized in their systematic review that tooth brush- effects from using different measuring systems and tooth-
ing practice reduces plaque from baseline plaque scores by brushes, and different gender, age and dental characteristics
42% on average, with a variation of 30—53%, dependent on of the study groups. Burgett and Ash discussed the significant
the plaque index used. In addition, they suggested that variation in the magnitude of brushing force (from 1.04 N g to
bristle tuft arrangement (flat-trim, multilevel, angled) and 11.3 N) when using different measuring systems, toothbrush
brushing duration were factors that contribute to the varia- grips, toothbrushes and techniques [49]. In addition, no
tion in observed efficacy. systematic review or evaluation of brushing forces has so
Recently, several studies have tried to evaluate brushing far been performed that compares different sextants and
techniques in terms of brushing motion, brushing action or tooth sites or of the effect of instruction on brushing tech-
both [33,37—40]. One study developed and evaluated the nique.
efficiency of a smart digital toothbrush monitoring and train- Very few studies have investigated the association
ing system in terms of correct brushing motion and grip axis between brushing force and gingival recession. The available
orientation in the at-home environment [41]. Their analyzing evidence suggests that tooth brushing force should not
software allowed calibration of all parameters individually. exceed 3 N to avoid gingival recession [50]. Tooth brushing
Tooth brushing for oral prophylaxis 73

abrasion is one factor in the multi-factorial process of tooth efficacy and effectiveness of various types of clinical inter-
wear. While tooth brushing is considered to be of minor vention. Indices for the clinical evaluation of dental plaque
importance for abrasion of sound enamel and dentin [51], have been developed and are listed in Table 3. Each index has
it was shown to be a significant risk factor for the etiology of its own characteristics, and each study selects the index best
erosive lesions [52—54], especially on eroded enamel and suited for its purpose. However, this variety of indices makes it
dentin. Tooth-brushing abrasion is determined by the abra- difficult to compare them directly for a systematic review. In
sive properties [55,56] and concentration [57] of the tooth- addition, studies using these indices typically rely on clinical
paste, and is also modified by the kind of toothbrush [58] and examiners to assign index values as a means of determining
the brushing force [59,60]. outcomes or for performing group comparisons. The quality of
Comparison of the brushing forces applied during in vivo the assigned values is dependent, to a large extent, on the
tooth brushing with manual and sonic toothbrushes and the skills of the examiners and on the validity of the assessment
average brushing force of manual toothbrushes (1.6  0.3 N) methods that are used. Therefore, much attention has to be
was significantly higher than for sonic toothbrushes paid to the common sources of error and bias that can lead to
(0.9  0.2 N) [54]. These different brushing forces affect difficulties in standardizing examiners [68].
the abrasive capacity of manual and sonic toothbrushes. Personal oral hygiene is often combined with therapeutics
Recent systematic reviews also suggest that power tooth- such as fluoride or xylitol, which have an effect on dental
brushes produce less clinically relevant damage potential to diseases and possibly tooth loss, making the evaluation of the
soft and hard tissues than manual toothbrushes [61,62]. pure effects of oral hygiene behavior difficult. Because the
Patients with clinically significant severe tooth wear and evidence for the effectiveness of these chemo-therapeuti-
exposed or eroded dentin surfaces should use sonic tooth- cals is sufficiently convincing that withholding them may be
brushes to reduce abrasion, while patients without tooth considered unethical. However, alternative delivery mechan-
wear or with erosive lesions confined to the enamel do not isms, such as chewing gum, may be viable, more practical,
benefit from reduced abrasion from sonic toothbrushes [54]. and more reliable than personal oral hygiene tools. Another
possibility is to evaluate variations in the level of personal
4.3. Duration of manual tooth brushing oral hygiene (much like studying varying levels of fat intake,
physical activity, etc.).
Duration is one of the critical factors affecting the efficacy of
tooth brushing for dental plaque removal [63]. It has been 6. Toothpaste and/or dentifrice
proposed that the main cause of insufficient oral hygiene in
the general population is too short a brushing time [64]. Advances in toothbrush technology are associated with more
However, changing this seems to be very difficult [65]. effective plaque removal, but excessive plaque regrowth can
Reported tooth brushing durations for children and adults also be a problem for individuals. Therefore, there is a need
are shown in Table 2. Brushing’s major effect on plaque for products that not only help users achieve optimal plaque
reduction is reached after 30—45 s of brushing per quadrant; removal, but also ensure that plaque levels remain controlled
accordingly, the commonly recommended tooth brushing overnight and throughout the day, thereby reducing the risk
times vary between 120 s (USA) and 180 s (Europe) [66]. of oral hygiene becoming suboptimal. The choice of denti-
Manual brushing for additional time did not lead to any frice had a significant effect on the inhibition of plaque
significant improvement [66], and a brushing duration of regrowth in a study with manual toothbrushes and may also
more than 3 min is usually not achieved [67]. On the other play an important role in optimizing the level of plaque
hand, Schlueter et al. [41] reported that repeat motivation at control achieved with power brushing.
each recall, using leaflets, instructions and demonstrations, The systematic review of the Cochrane database by Walsh
were statistically effective in prolonging tooth brushing et al. [69] confirmed the benefits of using fluoride toothpaste
duration and improving tooth brushing technique. to prevent caries in children and adolescents, but only for
fluoride concentrations of 1000 ppm and above. The relative
caries preventive effect of fluoride toothpastes increases
5. Clinical research for plaque removal with higher fluoride concentration. However, the choice of
efficacy fluoride level to use for children under 6 years should be
balanced with the risk of fluorosis. Haftenberger et al. [70]
The gold standard for clinical research is a randomized con- reported that most 1—3-year-old children in Brazil are
trolled trial with large numbers of participants to test the exposed to a daily fluoride intake above the suggested

Table 2 Reported toothbrushing durations.

Authors Year Age (mean age or range) Mean Duration (sec)


Tesini and Perlman 1994 8.4 57.8
Das and Singhal 2009 9—11 76.2
Sharma et al. 2012 8—12 85.8
Macgregor and Rugg-Gunn 1979 11—13 78.0
Macgregor and Rugg-Gunn 1985 18—22 33.0
Dentino et al. 2002 Adults 14 out of 81 spent 120 or more
74 H. Hayasaki et al.

Table 3 Indices for the clinical evaluation of dental plaque.

Authors Year Method


Quigley and Hein 1962 Quigley and Hein (Q&H) plaque index
Silness and Loe 1964 Silness and Löe plaque index
Turesky et al. 1970 Turesky modification of the Q&H plaque index
Elliott et al. 1972 Navy plaque index
O’Leary et al. 1972 O’Leary plaque control record
Lobene et al. 1982 Lobene modification of the Q&H plaque index
Rustogi et al. 1992 Rustogi modified Navy plaque index

threshold for dental fluorosis. The dentifrice alone was good oral health. Medications can induce hyposalivation,
responsible for an average of 81.5% of the daily fluoride which in turn, increases the risk of tooth decay and other
intake, while the diet, water and milk were the other most dental diseases [85,86]. Without support for regular oral
important contributors. Although the efficacy of fluoride hygiene habits there is a risk that dependent residents will
paste has been confirmed, information about its risks should develop oral diseases. Care involves not only caring for the
be given more emphasis. Accordingly, detailed longitudinal sick but also taking preventive measures to preserve good
studies of fluoride intake during tooth brushing with tooth- overall health.
paste in large populations are still needed, especially of very For individuals with disabilities, the dental health is gen-
young children in various countries and areas. erally worse than that of normal people of the same age
There is evidence that casein phosphopeptide-amorphous because their medical, physical, social, or psychological
calcium phosphate, CPP-ACP, can bolster the effects of disabilities limit their access to oral health care, including
fluoridated toothpaste alone to prevent caries. The localized diagnostic, preventive, interceptive and treatment services
CPP-ACP nanocomplexes in plaque and on tooth surfaces can [87,88]. Anders and Davis [89] concluded that patients with
buffer the free calcium and phosphate ion activities, main- intellectual disabilities have poorer oral hygiene than the
tain a state of supersaturation with respect to tooth enamel, general population [90—95]. The above listed disadvantages
prevent enamel demineralization, and promote reminerali- lead to poorer oral health, more untreated decayed teeth
zation [71]. Several studies have shown a synergistic effect of and severe periodontal status [96—99]. However, some stu-
CPP-ACP and fluoride in reducing caries [72,73]. Almost all dies [100,101] have reported that the oral health of children
clinical trials have investigated the effectiveness of CPP- with disabilities can be improved to a similar or even better
ACP-containing products in caries prevention and enhancing status than that of normal children at the same age in spite of
remineralization of initial caries lesions in the permanent their disadvantages. The main reasons for improvement can
dentition of young adolescents [74—76]. be accredited to the practice of daily tooth brushing by
Specially formulated oral care products containing stan- themselves or by their caregivers, and diet control by insti-
nous salts (e.g., chloride, fluoride) have shown to effectively tutes and schools [100].
protect enamel and dentin from erosive and abrasive wear in Dental care has been found to be the most common cate-
vitro [77—79] and in situ [77,80,81]. It has been suggested gory of unmet healthcare services for individuals with special
that the stannous ion can act either by precipitation on healthcare needs [102,103]. These individuals are at a higher
dental surfaces forming a relatively acid-resistant mineral risk for more untreated caries, gingivitis and other periodontal
layer or by incorporation into the eroded surface in a complex diseases, malocclusions secondary to abnormal development
demineralization and remineralization process [79]. While and muscle function, problems related to poor oral hygiene
these data are encouraging, it must be borne in mind that and dental/orofacial trauma, as well as inadequate access to
erosion is highly influenced by biological factors, especially care. [102] More importantly, if adequate oral hygiene and oral
those imposed by the acquired dental pellicle [82]. health care are not addressed the sequelae can be detrimental
to the overall health of the individual. The role of caregivers
7. Oral care for elderly persons and persons and dental health professionals’ instructions to caregivers are
with disabilities crucial for these disable persons.

Good oral health is an important aspect of quality of life, 8. Conclusion


even for the elderly [83]. Teeth are important for chewing,
speech and appearance [84]. In recent years, people’s overall In this paper we have discussed various aspects of oral
health has improved, as is reflected in an increase in the prophylaxis, especially, tooth brushing. This is a basic and
average life expectancy in developed countries. Today, the fundamental daily custom for almost all people, even in
elderly remain dentate to a greater extent than just a few developing countries, and its aim is to remove dental plaque.
decades ago and have more teeth, often with extensive However, more than 40% of plaque will not be removed, even
repairs, crown and bridge work and implants. This places by a well-trained person. Because of the importance of
high demands on satisfactory oral care for the elderly to prevention of dental caries and periodontal disease,
remain in good oral health. improvement of the quality of daily brushing is indispensable.
Oral health is also affected by a number of general health In addition, combining more effective brushing with dental
factors. Dementia and various mental and physical disabil- floss, inter-dental brush and oral rinses, will provide better
ities, for example, can result in difficulties in maintaining oral health for all.
Tooth brushing for oral prophylaxis 75

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