96 Fenmuzene ‘cock, Add 240 ml. 0,005 HCI to separator and reopen sty ‘cock, Open column stopcock until rate of ea 0.5 mb /min is Sustained 10 min, If rate decreases, increase rate slighlly until nearly const. Let elution continse overnight. Then, if >90 ml. remains in separator, readjust rate as above and continue elu tion to 90 mL mark. Finally, lowsr flask, fill 19 250 mL. mark at convenient rate from dispensing tip, and mix, Detach hose ‘and separator from column, and deain all 3. Reactive resin, ‘and stopper columo as in 973.01D. fe into 50 mL vol. Mask comtg small piece liens paper. Make alk. with 0.3N NOH, then just acidic With 0.25N HCL Pipet in 2 mL mote acid, 5 mL buffer, and 3 mL zincon soln. Dil. to vol. with H.O. Similarly prep. 0, 1, 2, and 3 ppm std soins, using 0, 5, 10, and 15 mL std Za Working soln, resp. Using 0 ppm std Soln as blank, det, A at {620 nm 15-5 min after zincon addn. Plot std curve of py against A, Zn = (Cx PW where C= ppm from std curve; W = g sample: (0.0625 for samples contg <0. 4% Zn of 6.25 ml. al petted onto resin for samples conte =0.14% Za, Ref.: JAOAC 56, 84611973), CAS-7440-66-6 (zine) 936.016 ‘Acic-Forming ‘or Nonacid-Forming Quality of Fertilizers Final Action Surplus 1970, See 2.141-2.142, Lh ed, 983.05 ‘Aluminum in Aluminum Sulfate-type Soil Acidifiers ‘Atomic Absorption Spectrophotometric Method First Action 1983, ‘A. Apparatus and Reagents (a) Atomic absorption spectrophotometer.—Petkin-Elmer Model 303, or equiv. See 972.06 for typical operating pas (b) Diluring soln, —Add 20 ml. H.S0, and 2.5 ¢ NaCl to 500 mL. H.0. Dil. to 1 L with HO. (©) Aluminum std solns.—U) Stock soln.—1 mg Ab/mt. (1000 ppm). Accurately weigh 1.000 g pure Al and dissolve in.ca 25 ml. HCL Evap, almost to dryness, add 500 ml. H.0. 20 ml. H5S0,, and 2-5 NaCl, and dil. TL with HO. @) Working solns.—Dil. aliquots of stock soln with dilg soln, (b), to make 4 std solns within range 50-150 ppm. 2B. Preparation of Sample Accurately weigh ca I sample into 500 mL. seres-cap er- enmeyer, add 250 mL HO, and shake on wristaction shaker cea 15 min. Quant. transfer to 500 mL. vol. flask and dil. to vol. with H.0. Filter thru Whatman No. 2 paper. Dil. aliquot as necessary (Giln factor = F) with dilg soln, (b), for eonen range 50-150 ppm. ©. Determination Set up app. as shown in Table 983,08, or use previously established optimum settings for app. Zero app. while aspir- ating dilg soln, (b). Det. A of std solns within 0-150 ppm AOAC OFFA MeTHCOS OF Ansys (1990) Table 983.05 Operating Parameters, Wiaviengt 2003 ‘sw en ‘ Source, me 20 NO. aiaeatng 45 (ecale visions) NiO, aunty and aspratng 55 (ecle isons) Ca te 8 (tal bal sale dvson) Fimo resueng Ht burer tat path, m ‘Sheipie uptake, mL/ma ‘ ‘Sptmam cone rang, o/m 0-150 range, alterating with sample soln readings. Flush burer with dllg soln, (b), and check zero point between readings Det. Al content from sid curve of 4 against wp Al/ml. % AV = (jag Al/mL) % F x (500/¢ sample) * 10~* Ref: JAOAC 66, 946(1982) ‘CAS-7429.90.5 (aluminum) PEAT 973.02 ‘Sampling of Peat Procedure 1973, (Moss, hummus, and recd-sedge types) Use slotted single or double tube oF sloted tube and rod, all with pointed ends and min. 1" diam. for loose materials Use cutting type core sampler, with plunger, for compressed materials. Pennsylvania State Forage Sampler (NASCO, 901 Janesville Ave, Port Atkinson, WI 53538) is satisfactory core sampler. ‘Take representative sample from lot or shipment as follows: a) Packaged or hated peass, Lay bag of bale horizontally and remove core diagonally from end to end. From lots of 1 10 bags, sample all bags; from lots of =11, sample 10 bags. ‘Take 1 core from each bag sampled: except for lots of 4 bags, take diagonal cores from each bag and adknl cores 10 total 25 cores (b) Bulk samples. —Draw =10 cores from different re sioas. (€) Small containers (10 tb of less).—Take entire package: ‘Working rapidly to prevent moisture losses, reduce composite sample to =500 g (by 30) or 2 L (by vol.) by mixing on clean plastic or paper and quartering. Place sample in ait-t Sampling by random “grab” procedure is necessary if pat= ticle size range is to be dete or representative sample cannot be taken with core sampler as above, Refs.: Book of ASTM Sts (1971) Pts 11, 22, and 30, ASTM. 1D2973-D2978, D2980, and D944. JAOAC 56, 1541973), 967.02 Preparation of Peat Sample First Action 1967 Final Action 1978 Place representative field sample on square rubber sheet, pa per, or plastic. Reduce sample to amt required by quartering And place in moisture-proof container. Work rapidiy o prevent moisture losses. Ref.: JAOAC $0, 394(1967)