BIOINFORMATICA

SLC12A3

Grupa M1529
Rotaru Ana-Maria

Chisinau, 2020
Task1
Tabel 1. Rezultatele căutării informațiilor pe portalul NCBI
Database Database name Database description Number of records
category

Literature Bookshelf full-text journal articles 10

PubMed Central full-text journal articles 766

Pub Med full-text journal articles 486

MeSH full-text journal articles 4

Genes Gene Tabular 320

GEO Profiles full-text journal articles 6365

GEO DataSets full-text journal articles 8

Homolo Gene full-text journal articles 2

PopSet Risk factor articles 1

Proteins Structure full-text journal articles 227

Identical Proteins full-text journal articles 15

Groups

Protein full-text journal articles 805

Genomes BioProject full-text journal articles 1

 BioSample full-text journal articles 7

SRA full-text journal articles 6

Nucleotide full-text journal articles 1125

Genome full-text journal articles 90

Probe full-text journal articles 404

Genetics dbVar full-text journal articles 254

ClinVar full-text journal articles 317

GTR full-text journal articles 71

MedGen full-text journal articles 4

dbSNP full-text journal articles 13829

OMIM full-text journal articles 20

Chemicals BioSystems full-text journal articles 159

PubChem BioAssay full-text journal articles 16

PubChem Substance full-text journal articles 115

LISTA DE REFERINȚE

1: Genome-wide association and Mendelian randomisation analysis provide insights into the
pathogenesis of heart failure
Sonia Shah, Albert Henry, Carolina Roselli, Honghuang Lin, Garðar Sveinbjörnsson, Ghazaleh
Fatemifar, Åsa K. Hedman, Jemma B. Wilk, Michael P. Morley, Mark D. Chaffin, Anna Helgadottir, Niek
Verweij, Abbas Dehghan, Peter Almgren, Charlotte Andersson, Krishna G. Aragam, Johan Ärnlöv, Joshua D.
Backman, Mary L. Biggs, Heather L. Bloom, Jeffrey Brandimarto, Michael R. Brown, Leonard Buckbinder,
David J. Carey, Daniel I. Chasman, Xing Chen, Xu Chen, Jonathan Chung, William Chutkow, James P.
Cook, Graciela E. Delgado, Spiros Denaxas, Alexander S. Doney, Marcus Dörr, Samuel C. Dudley, Michael
E. Dunn, Gunnar Engström, Tõnu Esko, Stephan B. Felix, Chris Finan, Ian Ford, Mohsen Ghanbari, Sahar
Ghasemi, Vilmantas Giedraitis, Franco Giulianini, John S. Gottdiener, Stefan Gross, Daníel F. Guðbjartsson,
Rebecca Gutmann, Christopher M. Haggerty, Pim van der Harst, Craig L. Hyde, Erik Ingelsson, J. Wouter
Jukema, Maryam Kavousi, Kay-Tee Khaw, Marcus E. Kleber, Lars Køber, Andrea Koekemoer, Claudia
Langenberg, Lars Lind, Cecilia M. Lindgren, Barry London, Luca A. Lotta, Ruth C. Lovering, Jian’an Luan,
Patrik Magnusson, Anubha Mahajan, Kenneth B. Margulies, Winfried März, Olle Melander, Ify R. Mordi,
Thomas Morgan, Andrew D. Morris, Andrew P. Morris, Alanna C. Morrison, Michael W. Nagle, Christopher
P. Nelson, Alexander Niessner, Teemu Niiranen, Michelle L. O’Donoghue, Anjali T. Owens, Colin N. A.
Palmer, Helen M. Parry, Markus Perola, Eliana Portilla-Fernandez, Bruce M. Psaty, Regeneron Genetics
Center, Kenneth M. Rice, Paul M. Ridker, Simon P. R. Romaine, Jerome I. Rotter, Perttu Salo, Veikko
Salomaa, Jessica van Setten, Alaa A. Shalaby, Diane T. Smelser, Nicholas L. Smith, Steen Stender, David J.
Stott, Per Svensson, Mari-Liis Tammesoo, Kent D. Taylor, Maris Teder-Laving, Alexander Teumer,
Guðmundur Thorgeirsson, Unnur Thorsteinsdottir, Christian Torp-Pedersen, Stella Trompet, Benoit Tyl,
Andre G. Uitterlinden, Abirami Veluchamy, Uwe Völker, Adriaan A. Voors, Xiaosong Wang, Nicholas J.
Wareham, Dawn Waterworth, Peter E. Weeke, Raul Weiss, Kerri L. Wiggins, Heming Xing, Laura M.
Yerges-Armstrong, Bing Yu, Faiez Zannad, Jing Hua Zhao, Harry Hemingway, Nilesh J. Samani, John J. V.
McMurray, Jian Yang, Peter M. Visscher, Christopher Newton-Cheh, Anders Malarstig, Hilma Holm, Steven
A. Lubitz, Naveed Sattar, Michael V. Holmes, Thomas P. Cappola, Folkert W. Asselbergs, Aroon D.
Hingorani, Karoline Kuchenbaecker, Patrick T. Ellinor, Chim C. Lang, Kari Stefansson, J. Gustav Smith,
Ramachandran S. Vasan, Daniel I. Swerdlow, R. Thomas Lumbers
Nat Commun. 2020; 11: 163. Published online 2020 Jan 9. doi: 10.1038/s41467-019-13690-5
PMCID: PMC6952380

2: Dataset on the mass spectrometry-based proteomic profiling of the kidney from wild type and the
dystrophic mdx-4cv mouse model of X-linked muscular dystrophy
Paul Dowling, Margit Zweyer, Maren Raucamp, Michael Henry, Paula Meleady, Dieter Swandulla,
Kay Ohlendieck
Data Brief. 2020 Feb; 28: 105067. Published online 2020 Jan 2. doi: 10.1016/j.dib.2019.105067
PMCID: PMC6956748

3: Refining modules to determine functionally significant clusters in molecular networks

Rama Kaalia, Jagath C. Rajapakse
BMC Genomics. 2019; 20(Suppl 9): 901. Published online 2019 Dec 24. doi: 10.1186/s12864-019-
6294-9
PMCID: PMC6929267

4: Chronic kidney disease: a review of proteomic and metabolomic approaches to membranous

glomerulonephritis, focal segmental glomerulosclerosis, and IgA nephropathy biomarkers
Amir Taherkhani, Reyhaneh Farrokhi Yekta, Maede Mohseni, Massoud Saidijam, Afsaneh Arefi
Oskouie
Proteome Sci. 2019; 17: 7. Published online 2019 Dec 20. doi: 10.1186/s12953-019-0155-y
PMCID: PMC6925425

5: The Urinary Excretion of Uromodulin is Regulated by the Potassium Channel ROMK

Guglielmo Schiano, Bob Glaudemans, Eric Olinger, Nadine Goelz, Michael Müller, Dominique
Loffing-Cueni, Georges Deschenes, Johannes Loffing, Olivier Devuyst
 Sci Rep. 2019; 9: 19517. Published online 2019 Dec 20. doi: 10.1038/s41598-019-55771-x
PMCID: PMC6925250

6: Case Report: Investigation and molecular genetic diagnosis of familial hypomagnesaemia

Jamie Willows, Maryam Al Badi, Chloe Richardson, Aisha Al Sinani, Noel Edwards, Sarah Rice,
John A. Sayer
Version 2. F1000Res. 2019; 8: 666. Published online 2019 Dec 5.
doi: 10.12688/f1000research.19006.2
PMCID: PMC6694456

7: The Molecular Genetics of Gordon Syndrome

Holly Mabillard, John A. Sayer
Genes (Basel) 2019 Dec; 10(12): 986. Published online 2019 Nov 29. doi: 10.3390/genes10120986
PMCID: PMC6947027

8: Single cell census of human kidney organoids shows reproducibility and diminished off-target
cells after transplantation
Ayshwarya Subramanian, Eriene-Heidi Sidhom, Maheswarareddy Emani, Katherine Vernon, Nareh
Sahakian, Yiming Zhou, Maria Kost-Alimova, Michal Slyper, Julia Waldman, Danielle Dionne, Lan T.
Nguyen, Astrid Weins, Jamie L. Marshall, Orit Rosenblatt-Rosen, Aviv Regev, Anna Greka
Nat Commun. 2019; 10: 5462. Published online 2019 Nov 29. doi: 10.1038/s41467-019-13382-0
PMCID: PMC6884507

9: Interaction between ELMO1 gene polymorphisms and environment factors on susceptibility to

diabetic nephropathy in Chinese Han population
Yi Hou, Yong Gao, Yan Zhang, Si-Tong Lin, Yue Yu, Liu Yang
Diabetol Metab Syndr. 2019; 11: 97. Published online 2019 Nov 27. doi: 10.1186/s13098-019-0492-
0
PMCID: PMC6882154

10: Machine Learning Approaches for the Prioritization of Genomic Variants Impacting Pre-mRNA
Splicing
Charlie F Rowlands, Diana Baralle, Jamie M Ellingford
Cells. 2019 Dec; 8(12): 1513. Published online 2019 Nov 26. doi: 10.3390/cells8121513
PMCID: PMC6953098

Table: Phenotype-Gene Relationships

 Phenotype Gene Number
Phenotype
Location Phenotype Inheritance mapping MIM Gene /
MIM number
key Locus Locus

16q13 Sindromul Gitelman 263800 AR 3 SLC12A3 600968

Table: Allelic Variants

Număr  Fenotip  Mutaţie  SNP gnomAD ClinVar

0.0001 SINDROMUL SLC12A3, rs121909379 rs121909379 RCV000805008 ...

GITELMAN LEU850PRO

0.0002 SINDROMUL SLC12A3, rs28936387 rs28936387 RCV000009116

GITELMAN CYS421ARG

0.0003 SINDROMUL SLC12A3, rs28936388 rs28936388 RCV000009117

GITELMAN ARG209TRP

0.0004 SINDROMUL SLC12A3, - - RCV000009118

GITELMAN IVS15AS, GT,
-1

0.0005 SINDROMUL SLC12A3, rs121909380 rs121909380 RCV000009119 ...

GITELMAN ARG655HIS

0.0006 SINDROMUL SLC12A3, rs121909380 rs121909380 RCV000009120

GITELMAN ARG653LEU
Număr  Fenotip  Mutaţie  SNP gnomAD ClinVar

0.0007 SINDROMUL SLC12A3, - - RCV000009121

GITELMAN IVS23DS, GT,
+1

0.0008 SINDROMUL SLC12A3, rs121909382 rs121909382 RCV000009122

GITELMAN ALA588VAL

0.0009 SINDROMUL SLC12A3, rs121909383 rs121909383 RCV000009123 ...

GITELMAN PRO349LEU

0.0010 SINDROMUL SLC12A3, 3-BP - - RCV000009124

GITELMAN DEL

0.0011 SINDROMUL SLC12A3, rs121909384 - RCV000009125

GITELMAN GLY630VAL

0.0012 SINDROMUL SLC12A3, rs121909385 rs121909385 RCV000009126

GITELMAN LEU623PRO

0.0013 SINDROMUL SLC12A3, rs267607051 rs267607051 RCV000009127

GITELMAN THR163MET ȘI
ARG871HIS

0.0014 SINDROMUL SLC12A3, 2-BP - - RCV000009128

GITELMAN DEL, 2881AG

0.0015 SINDROMUL SLC12A3, rs371443644 rs371443644 RCV000489628 ...

GITELMAN THR60MET
Table: Clinical Synopsis
MOŞTENIRE
- Autosomal recesiv

ABDOMEN
- Dureri abdominale (la unii pacienți)

genitourinare
Rinichi
-
- renală de potasiu
- renală de magneziu

SCHELETIC
- condrocalcinoză

ȚESURI DE MASCĂ, SOFT

- Slabiciune musculara generalizata
- Crampe musculare
- Tetanie

NEUROLOGICE
Sistem nervos central
- Convulsii
- paresteziile
- , episodică, după exercițiu intens

CARACTERISTICI METABOLICE
- hipocalemică alcaloză
- Polidipsie

CARACTERISTICI ENDOCRINE
- plasmatică crescută

ABUNE DE LABORATOR
- Hipopotasemia
- hipomagnezemia
- Hipocalciurie

DIVERSE
- Debut în copilărie (mai târziu decât în sindromul Bartter antenatal 241200 )
- Prevalență de 19 la 1.000.000 în Suedia
- Prevalență de 1 din 40.000 în rândul caucazienilor
- Transportatorii heterozigoti au scăzut tensiunea arterială comparativ cu generalul populație

BAZĂ MOLECULARĂ
- cauzat de mutație în cotransporter Na-Cl sensibil la tiazidă (SLC12A3, 600968.0001 )
ClinVar

Gene Clinical
Protein
VariationLocation Condition(s) significance Review status
change
(s) (Last reviewed)

NM_000339.3(SLC12A3):c. SLC12A3 T60M not provided, Pathogenic criteria provided,

179C>T (p.Thr60Met) Familial (Nov 9, multiple
GRCh37: hypokalemia- 2018) submitters, no
Chr16:56899326 hypomagnesemia conflicts
GRCh38:
Chr16:56865414

Task2
Official Symbol
SLC12A3provided by HGNC
Official Full Name
solute carrier family 12 member 3provided by HGNC
Primary source
HGNC:HGNC:10912
See related
Ensembl:ENSG00000070915 MIM:600968
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires;
Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
NCC; TSC; NCCT
Summary
Această genă codifică un cotransporter renal sensibil la clorură de sodiu tiazidic, care este important pentru
homeostazia electrolitului. Acest cotransporter mediază reabsorbția de sodiu și clorură în tubul convolut
distal. Mutațiile acestei gene determină sindromul Gitelman, o boală similară cu sindromul Bartter, care se
caracterizează prin alcaloză hipokalemică combinată cu hipomagnezemie, calciu urinar scăzut și creșterea
activității reninei asociate cu tensiunea arterială normală. Acest cotransport este ținta pentru diureticele
tiazidice care sunt utilizate pentru tratarea tensiunii arteriale ridicate. Au fost găsite mai multe variante de
transcriere care codifică diferite izoforme pentru această genă.. [provided by RefSeq, Jul 2008]
Expression
Restricted expression toward kidney (RPKM 65.2)
NCBI Reference Sequences (RefSeq), Genomic
Secvența nucleotidică a genei
>NG_009386.2:5001-55644 Homo sapiens solute carrier family 12 member 3 (SLC12A3), RefSeqGene on chromosome 16
CTGGCCCCTCCCTGGACACCCAGGCGACAATGGCAGAACTGCCCACAACAGAGACGCCTGGGGACGCCAC
TTTGTGCAGCGGGCGCTTCACCATCAGCACACTGCTGAGCAGTGATGAGCCCTCTCCACCAGCTGCCTAT
GACAGCAGCCACCCCAGCCACCTGACCCACAGCAGCACCTTCTGCATGCGCACCTTTGGCTACAACACGA
TCGATGTGGTGCCCACATATGAGCACTATGCCAACAGCACCCAGCCTGGTGAGCCCCGGAAGGTCCGGCC
CACACTGGCTGACCTGCACTCCTTCCTCAAGGTAAGTGCTGTCTCAGAAGACTGGCCACTTCCCTGCTGT
GTGACCTCGACCCAGCTACCTAACCTCTCTGAGCCTCAGTTCTGTCATCTGTGCCATGGGGATGGAGGAG
CGTCTTCTTCCTGGGTTGAGAGGCCCCAGTGAAATAGTGGAGCTGCAGAAATCTGGGCACATGTTGGAAT
GTATATTAGCCAATCACTAGCAACCCAAAGCTAGAGTGGGAGGCCTAGAGAGGTAATGAGCTTCCAGTGA
GTAGAAGCATGCAAGCAGGTTGCACACCCCTGAAAGAGACAGGGCAAGAAGGGCCTGGGAAGGTTCACCT
CAGAGAGAGGGCTCGCCGGGTTTGCTGCCCGGGAGGCTGGACTGTGAAGAGTGGAGGGCTGGAAGGTGGA
TTGTCATGACCCCTGTCTGGCCTCACTTTTCCCATATGACAATTGGGCTGAATTGTTCTTTTTTCTTTTC
TTTTCTTTTCTTTTCTTTTTTTTTTTTGAGACGGAGTCTGGCTCTGTCGCCCAGGCTGGAGTGCAGTGGT
GTGATCTCGGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAG
CTGGGATTACAGGAGCGCACCACCACACCCAGCTAATTTTTGTATTTTTAGTAGGGATGGGGTTTTGCCA
TATTGGCCAGGCTGGTCTTGAACTCCTGACCTCAAAGGATCCACCCACCTTGGCCTCCCAACGTGCTGGA
ATTACAGGCATGAGCCACCACGCCCAGCCCAGAATTGTTCTTGATTCTGAGGCTAAGACTTACTAAATTC
GCAGGCCTTTTCTCCACCCCAGAGCAAACCAAGACATGGCCCTTGGCTCCATACTGAGATGAAGTGCCAG
GAAGAAAGCCCCAGAGGAGGGCGTCAGTCACTTGGGGCACAAACTAGGAGACCTCCCAGTGCCCCAGAGG
TAGAGGCCTGGTTTCAAGACCCAGCCCTCCAGGGACCAACTATGTGGCCTTGGACAAGCTTTCACCTCTC
CAGGGTGATAATAATCCTGTCGCCCATTACAAGGCTAGGCAACTGCCTAATCACTTCTAAGGCCACTGCT
CTCCTAGGGGGAGGAGGTGTTATGAGCAGGAGCGCCCAGGACTCCCAGTCTCCCATGGCATCTGATCTTG
GATCACTGCAACCTCCACCTCCTGGCTTCAAGAGATCCTCCTATGTCAGCCTCCTGAGTAGCTGGGACCA
CAGGTGCACACCACCATGCCCAGCTAATTTTTGTATTTCTTAGTAGAGACAGGGTTTTACCTTGTTGCCC
AGGCTGGTCTTGAACTCCTAAGCTCAAGCAATCCACCCACCTTGGCCTCCCAAAGTGCTTTTACTACAGG
CGTGAGCCACTGCTCCTGGCCTAGGGAGCATTTTAAAAGCCCCTCAAGCAGCTCAACACCCAGTGGGCTG
AGGGGTCGGGGGGTGCTCGGTATGGGGCGCAGTGGTGCAGGTCAGTGGGCTGGATGCAGAGACGCCGTCC
CTAGCACCCCTACCTGCCTGACTTGTGGTCTCTGGGCTGCCAGCAGGAAGGCAGACACCTGCATGCCCTG
GCCTTTGACAGCCGGCCCAGCCACGAGATGACTGATGGGCTGGTGGAGGGCGAGGCAGGCACCAGCAGCG
AGAAGAACCCCGAGGAGCCAGTGCGCTTCGGCTGGGTCAAGGGGGTGATGGTGAGTGGGGTGTGGGTGGT
GCGTGATGTCCAGAAATGGGGGTGGGGTGGCAGAGCTCCATCCAGGCTCAGCTCTGACTCTCAGGCCCTG
GTGGGGCTTCAGTTTCCCCATCTGTACAATGAATTCAATGAGTTAATAGATCAATAGACAATAGATTAAA
GCCTGCCGGGGAGTAATTAGAAGAAACTGTGTTTCCCTAAAAAGCCACCAGGGGGCTGCACTAAACACAC
AAATCCGTAAATGTGTTTTTCTCCTCCATGAAGGGAAGGAAAAGGTTTGCTATAGTGAGGAGCAGGATTA
AGTTTCCAGACAGTAGACAATCAAGGACACTTGGCTTTGGAGACATTATGGGACTTTACAGAGGGGATCT
TATGGGATGTAGAAGGCTTTAGCCCTGCTCCGGGAGGCAGGAGGCTGGGGTTCTGATCTTAACGCTGCCA
ACTGGCTGTGCCTCCGTTTCTCCATCTGAACCACGAGCCCTCCCCGACCCCCAGATGCTCTTATTCAGAG
CTGGGATCTCCCTCCTGGGCTGTGTTGCCAGAGCCGGGAACGGCCCTGCAACTCAGTGGGGCCAGCGACC
ACACCAGCCCCTCCCACCTGGAAGCTTCAGCCTCCATCTCGGCCTCTGGCTTTGCTTTAAATTTGGAAGC
TGAAGTCACAGCCACCTGAGCTCTGATATCTGCTCTTACATGGGGCGCTGGCTCACCTGGCATTTGCTGA
GCTCCCTGTAAATGCCAGGCTCTGGATCAACTGCTGACCACTGGGGCCATGGTCGGGGCTGGCAGACACA
GGATCCTGGGCTTGGGATGGATACAGTGAGGGCTAACAGTGCTGGTAGCCTAGCGTCAGGACTCAACGGG
AGGGTGGGAAAATAGGTGGGAGGGATAGGTGGGTCTCTGGGTGCCTCTCACCCTCTTGCCCCATAGAACC
AGACTCGGAACCTACTGAAGTGGGTGAAGAAGGGACCCAGGTGTCCCTAGGGCCTAGGTGCTCGATACCC
TGCCATAGCAAGGGACAGGGACTTGTCGTTTGGCCTTGGGGTGTCCACCCAGGTGGCCTCTGACCCCCCT
GTCCTCCCAGATTCGTTGCATGCTCAACATTTGGGGCGTGATCCTCTACCTGCGGCTGCCCTGGATTACG
GCCCAGGCAGGCATCGGTGAGTGCCCCTCTGGGGAAGAGGAGGGAGGGCTTGCCTGAATCCCATTCTTCC
CAGCTTGCCTGAATCCTGTTCTTCCCAGACCCCAAGGTTGCAGGATTAAACTTGAGGTGCAGAGACCAAA
CGGCCAGGCCTTCTCCTCCCTGGTTCAGTCTCAGGCCCCTGCAGTGACAGGGGTCAGGGTGGTGTGGCAG
AGGCACGCTGGACATGGGGTCAGCAGGCCTAAGGGCTATTTGCCCTCAGTAAGCCACTTAACCTCTCAGA
GCCCATTTCTACCTCAAAGCGTGCAGGTTAGAAAGCTGCACTCTGGCCGGGCACGGTGGCTCACGCTTTG
GGAGGCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGTGGATCACGAGGTCAGGAGTTCAAGACCAGC
CTGGCCAACATGGTGAAACCCTGTCTCTACTAAAAATATTAAAAAATTGGCCAGGCGTGGTTGGCAGGTG
CCTGTAATCCCAGCTACTCGGGAGGCTGAGTCAGAGAATTGCTTGAACCTGGGAGGCAGAGGCTGCAGTG
AGCCGAGATCACGCCACTGCATTCCAGCCTGGGCAACAGAGTGTGAAACTCCATCTCAAAAAAAATAAAA
AATAAAAAAGAAAGCTGCATTCTGGACAGAACAGGACTCAGATGCTAGCTCTGTAGCCTGCTATGTGACT
TTGGGCAAGTTTCTTAACCTCTCTGAGCCTTGGATTCCTCATTAGGAAAATGAGGGTGATAACAGAACCT
ACCTCATGGAACCGTTGTGGCTTTTACAGGATGCAAGTAAAAACACAGCGCAGATTTGGCTCCCAGAGGT
GATCACGGAGTGGTAACTGCTACTTTATTTTTTATTATTAAACGAGGGTAGCTTCCCCTCCATACCTAAC
AATCCAATAAATGCTGGCTCAAGGGCTCACTGGCTATTCAGAGTATGACAGGAGGTAGACAGAGACCCAT
TTTTTTTTTGAGACGAAGTCTCACTCTGTCACCCAGGCTGGAGTGCAGTCGCGTGGTCTCGGCTCACTGC
AACCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTAAGTAGCTGGGATTACAGGCATGT
GCCAGCATGCCTGGCTAATTTTTGTATTTTTAGTAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTCT
TGAACTCCTGACCTTATGATCTGCCCGCCTCGGCCTCCCAAAGTGACAGAGACCCATTTTTCAGATGAGA
AAACTGAAGCTCAGAGAAGGGAGATGAACGTAGGTCGCATGGTGAATGAGTAGGCAAACTGGGGCTCCTC
CCTTGGGAAATGCCCTGCCTAAGCTTTGGGTGCCCCCTGCAGTCCTGACCTGGATCATCATCCTGCTGTC
GGTCACGGTGACCTCCATCACAGGCCTCTCCATCTCAGCCATCTCCACCAATGGCAAGGTCAAGTCAGGT
GGGCCATCCCCCTTTCCACCAAGGCCCTCCTCTCGTGGGCTCCTAATCCTGGGAACAGGACTCCCAACTT
CCCCAACCCAATGGTACACCCAGCCGCTCCTGTGGTTCCGGGAGAGGGCTCTAGGCAGGCTGTCTACACC
ACGAGATGGCCTCAGCTATCTCCTGCCCCGTGGGTCCTGTGGCCACCCTCTCCTGGCCTCTGCCTGCCCT
GAGTCCACCCCAGCCAACCGACTCATCTGGTTTCATGGTTCCCGGCTCTGCCCTGATAGGTGGCACCTAC
TTCCTCATCTCCCGGAGTCTGGGCCCAGAGCTTGGGGGCTCCATCGGCCTCATTTTCGCTTTCGCCAATG
CCGTGGGTGTGGCCATGCACACGGTGGGCTTTGCAGAGACCGTGCGGGACCTGCTCCAGGTGAGGCCGGG
GGGCTGGACCCTGGGTAGAGGGATCCGGGCAGCCCATTGCACTCTCCTCCGCCCCATCTGGGCTGGGGCC
TCCTGCTGCTCTGCCTGACTTCACCCATCAGGAACCACAGCCTGATCACTGTGGGTGAGAGTGACAATCT
CATCAAATAAAGGCCATTGCTTTCCCACAAGTCCCCTTTGGGGGTGTGTGTGGCCTTTAGAGCCACACTG
TCCAGGCCCGTGCAGCAGCTGCTTTGGGGACTCGATGGCAGGGGTGGTGCTTGAGTTAACATCGTCCTAG
CAGAGTGCACCGCACAGGAGGTGCCTCCTAGGTGGGCAGAGTCTGGGGGATGGGGAATTCAGAGGGTGGC
TTGCAGCCTGGCCCATTTTCCCTCCCCAGGAGTATGGGGCACCCATCGTGGACCCCATTAACGACATCCG
CATCATTGCCGTGGTCTCGGTCACTGTGCTGCTGGCCATCTCCCTGGCTGGCATGGAGTGGGAGTCCAAG
GTGAGGAGGCCATGGAGGAGGGGGACATGGAGGTGGTCACGTGGAGAAGCGGGGGTTGCCAGGCCTGGGC
CCTCCCTGGTCCTCTGCCTTTTCTTTTTCTTTTCTTTTTTTTTTTTTGAGACAGAATCTCGCTCGATGTC
CATGCTGGAGTGCAGTGGTACCATCTCGGCTCACTGCAACCTCCATCTCCCAGGTTCAAGAGATTCTCCT
GCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCACCTGCCACCACACCTGGCTAATTTTTATATTGTTTG
TAGAGACGGGGTTTCACCATGTTGGTCAGGCTGGTCTCAAACTCTTGACCTCAAGTGATCTGCCCGCCTT
GGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGTGCCCTGCTGCTTTGTATCTCTTGTGTGACA
ACCTAGGTCCACATGGCCCAGGGTGGGCATGGTGGCTCAGGGGGCCGGGCACCCAGACTCCTTCCATCTT
GTGCTCCCTCCATCCCTGCGCCGTGGCCTTTGTCCACCTGGTCCACCATACCCACATCCCCTCCAGTCAG
AGGGAAAGCAAAGAAGGGGGAAATGAAAGGGAGGGAATTCCCTTTTCTCTTTCTTTTTATGGCACCATCT
GGAAGTTTCACTATCGCTGTCACACATGGCTGCGAGGGAGCCTGGGGAGGTGTGGCCTTCAACTGAATCA
TGGATCCTGCTGGCAGACTCAGCCGGACAGGCCCTACAGGCAGGGCAGGACCTGGCCCCTGCAGCCTGTC
CTCTCCCCGCATTTTCTGACCTGCAGCTGTCAGAGGGTGTCCTCCTTGCTCTTCCTTGGATGCCCTGAGC
TGAATTCCCTGGACAGTTCTCTGGCTTGTTCATTCTCCCACTTAAGGTGTCTGCTAAACACCTTCTCTCT
GGTGACACCTTCCCAGGTCATCCTGCTAAATGAGCTCCACCCAGTCCCCATCACACTCCCTCTTCTTTAT
TTATTTATTTATTTATTTATTTTTGAGATGGAGTCTCGTTCTGTCACCTAGGCTGGGGTGCAGAGGCGTG
ATCTCGGCTCACTGCAACCTCCGCCTCCTCGTCTCAAGCAATTCTCCTGCCTCAGCCTCCCAAGTAGCTA
GGATTACAGACACCCACCACCATGCCTGGCTAATTTTTGTATTTTTAGTATAGACGGGGTTTCACCATGT
TGGCCAGGCTGATCTCGAACTCCTGACCTCAGGTGATCCGCCCACCTCGGTCTCCCAAAATGCTGAGATT
ACAGGTTTGAGCCACTGTTTCCGGCCTGTATTTTTAGTAGAGACAGAGTTTCCCCATGTTGGCCAGGCTG
GTCTCAAACTCCTGACCTCAGGTGATCCGCCCACCTCGGCCTCCCAAACCTCTTCTTACTTTAAATTCAT
CCTCCTTATTTATCAGCCAAGACAAGAGGCTTTGGCGGTCTTGTTCACTGCTATATTCCCAACTCCTTGA
ACAGATCCTGCTGCATAATGGGTGTTGAATGAATGAGTGAGTGAATAATGGAGAAACGGGCCCTGGGCAA
ATCATTTTCTGGCGGGGCTTCCCAGAGAGGTAGAACAAAACTATCTGACCTCTGGGGTCCTTCGCCCCCT
CCAGGCCCAGGTGCTGTTCTTCCTTGTCATCATGGTCTCCTTTGCCAACTATTTAGTGGGGACGCTGATC
CCCCCATCTGAGGACAAGGCCTCCAAAGGCTTCTTCAGCTACCGGGGTATGTGCTGATCAAGGCCCTGAC
CATGGCTCTGGGGACAGGGACTCTCTACCCAGGAATCTGGCCCATTGTGTGGACACTGGGAGGATGGGAT
TACCCAATCCCATGGTCAGGGGCTGCCTGCCCAGTGGGTCCCAGCAAGGGGGGTCAAGCCCTCCAGGTGA
GCCTTACTCATCAGGCCTTGCTTTTCCAGCGGACATTTTTGTCCAGAACTTGGTGCCTGACTGGCGGGGT
CCAGATGGCACCTTCTTCGGAATGTTCTCCATCTTCTTCCCCTCGGCCACAGGCATCCTGGCAGGGGCCA
ACATATCTGGTGACCTCAAGGTGAGCAGAATACTTGCCCCTCCTGTGTCCTGGCACTGCACAGGGGCTAT
GAGCAGAATCCTGCCCCCTGCTCTAGTGGCATCTGCCGCTGACCTGGGAGGCAGGGCTTCTAAAATCCAG
TCCAGTCCAACTCAGCTCAGTTCAACCCAATCCAACCGATTCCATTCCGTTCTGAGGTTGTTTATGAAGA
CCAGCTGCGTTCTAGGCTTGAGGGACACCACAGAATGGCTAGGTTCCTCCCTAGTTCCCAGAACTCTCAG
CCTTAGGGGAAGACTGGCCCAGCTGTGACCTTAAGAGCCCAGCCTTGCTCATGCACATCTTTGCTGGGCT
GACATCCTCCCAGGGCTCCCCACTGCCTTGCTGCAGGCAGGCTTCTTGGCTTGGCATTCAAGGCTCCAGC
CTGCCTGTCTAGCCTCATCCCTGACCAGTGCCACCCTTCCCATGAGCCTGCTGCAGCCCTGAGGCTCTAC
TGCTTTGCCCGTGTTACCTCGTCCCTCCTCCACGCCTTTGCCTGTGCTGCGCTCTCTACCTGCAACTCCG
TACGCTTGCTTTTTTACCCTGAAAGTCTGTTCTGTTTCTCTTTCTTTCTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTGAGATGGAGTTTTGCTCTTGTTGCCCAGGCTGGAGTGCAATGGCATGATCTTGGCTCACTGCA
ACCTCCGCCTCCCGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGTGCCTG
CCACCGCACCCGGTTAATATTTGTATTTTTTAGTAGAGATGGAGTTTTGCTATGTTGGCCAGGCTGGTCT
CAAACTCCTGACCTCAGGTGATCCATTCGCCTGGGCCTCCCAAAGTGCTGGGATTATAGGGGTGAGCCAC
CATGCCTAGCCTCTTTTATTTATTTTATTTTATTTTTTTTTTTTTGAGATGAAGTCTCGGTCTGTCGCCC
AGGCTGGAAGGCTGGAGTGCAGTGGCACGATCTCAGCTCACTGCAACCTCCGCCTCCCGGGTTCAAGTGA
TTCTCATGCCTCAGCCTCCAGAGTAGTTGAGACTACAGGCACCTGCCACCACGCCTAACTAATTTTTGTA
TTTTTAGTAGAGACAGGGTTTCGCCATGTTGGCCAGGCTGGTCTCGATCTCCTGACCTCAAGTGATCCAC
CCGATTTGTCTTCCCAAAGTGCTGGGATTACAGGAGTGAGCCACTTCTCCTGGCCTGTTTCTCTTTCAAA
TACCGCCTTCAACTATCATCTCTCCCAGCCCTTCCATCAGAGTCATGGTTCCCCAGCATAATGATGTTGG
GCCAAACGCGTATCTGTTGCCCCGGCATCTGGAGAACGGAGACTTGCCTCTGTCTCCCTATTCCCTCCCA
GCTCAGAGCCTGGCACATACTAGGTGCTCTGTACATGTTTGTTGACTGAATCATGGTGAAATGCAGTGTT
TGAGCAGTAGCACAAAGTGCTGCACCAGCAGGGAGAAGGAGAGACTGATGTTGGCAGGGGTTCCAGCAGA
ATTTCTGGGTGCAGGAGGCGCCAGAGCTGGGCTTTGAAAGGTGACAAGCTGGCAGAACCTTTGCTCAAGG
GAGGTGGCAGGCAAGAGGGGGTGATGAATGGGGAGCTAGCCCCACACTGGAAATCACAGCTAACCCCTAC
CCCAAACCTCCAGTCTCATTTGGAAGGCGCTCCTTGATGCATTTTCTTAAGCAAATTAAGAGCTGTGCAT
TGAGCCAGGCACAGTGGCTCATGCCTGTAATCCTAGCACTTTGGAAGGCCAGAGCAGGTGGATCACTTGA
GGTCGGGAGTTCGAGACCAGCCTGGCTAACATGATGAAACTGTTTTAGTCTCCACTAAAAACATAAAAAT
TAGCCGGGTGTGGTGGTGGGTTCCTGTAATCCCAGCTACTCGGGAGGCTAAGGCAGGAGAATTGCTAGAA
CCCGGAAGGCGGAGGTTGCAGTGAGCCAAGATGGCGCCACTACACTCCAGCCTGGGTGACAGAGCGGAAC
TCCATCTCAAACCTCACCCCCCCGCAAAAAAAAGGAATGCACATGGAGTCGACTGTGAAGCCAGCGTGGC
TTTAAGCAGCTATGCACCAGGTGGTGTTGGGCAGGGCCTGGAAATTGGAGAACAGCCGTTGGTTTGGCTC
CTCTCCTTTTTCTGGGGAATGGCCAGCAGAGGCCTGGTCTGCCTGCCCTGAGGTCCCGCAGAGGTTGGGC
CTGTGCTCAGACAGCCTGGATTCCATGCAGTCTCATTAGATCGTTGCCTGGGAATTTATCTCGATGCCTC
ATCAAGCTTTTTAGGTTACCAACCAGCAGGCGTCAGCTGTCTTGAAGCTGGATGAGGCCCGCGCGCCTGC
ACTCTGTCCTAAATGCCCTCAAGCTTTAAGGGGCTCTTGGATGGCCTCCACCCATCCTGTGGGACAGCAT
CCTCAGCAGCAGAGCCACTGTTTGTGGAGCCCTCACTGTGACATTTTGGGCAAGTCCCTTTAAAACTCCC
TGGGCCTCAGCTTCCCCAATTCTAAAATGGGACGAGTACAGCCCCCATCTTGCAGGGCGGTGGGGAGGAT
TCCATGTGCTAATTCATGTAAAGTGCTTAGAATAGTGCCTGGCTCAGAGCAGGAGCTAAGGGCTTCTTGT
TATCATTGTCATTTTTACCATGATATAGACAAGCAAGCCAAGGTCAGAGAGGTTCGGGGGCCTGCCCAGG
ACTACACAGGTGGCAGAGGAGCCGGAATGCCAGTTTCTAAAACAGAGAGGCCTCAACTACATCACAATTT
CATCATCTAACAAATACGTTTTGTGCTGCCTCAGGGCATGCCCCTGTGCACAATCCTGCCCCCACCCCAA
CAGCTCACATTTATGGGCACTTGTCACCTGCCAAGTTATTTTCTGATTTCACAGATGGGGAAACTGAGGC
TCAGGGATGTTAAGGGGCTTCTCCCAGTGCACACAGCAGGGAAGAGGTGGGCTGAGATGTGGACCTGGAT
GTGCCTAACGTTGTTAAATCCCCGGGCAACAATCTCAGAGTTAGGCACATCCAGGTCCACATCTCAGCCC
ACCTCTACCCAGCTCACCTCTTCTCTGCTTGCCTCTTCCCAGCACATCCAGAGGCTGAGATCTCAGCTCT
AGATGCAGGAGGCAGGATGGGGGCTGATGGCTGGTGTATTTGTGTTTTCACCACAAATTGGGTGGCTTAA
AAACAGACCTATATTCCCTCCCCGTTCAGGAGGCTAAAAGTCCGGGATCCAGGTGTCAGCAGGACCAAGC
TCCCCCTGAAAACCCAGGGGAGAATCCTGCCTTGCCTCTTCCTAATTTCTGGTGGCTGCTGACAGTGGCT
TTTTCTTGGCTGGCAACTGCATCACTCCAATCTCTGTCTCTGTCTCACGTGGCCTTCTTTCATCTGTGGG
TCCTTTCCTTGTAAGGACACCAGTCATTGCATTTGGGGCCCACCCTAATCCGGTATGACCTCATCTTTAC
TAACTGCACCTGCAAAGACCCTGTTTCCAAATAAGGCCACATTCTGAGGTTCTGGGTGGATGTGAATTTT
GGGGGACGCTATTCAATCCACTAGAGGCAGGGTGGGAGGCTGGTGTGTTCTGTTGGCTTTGGGGCTGGGG
ACCCCATGTGCATGGCCCAGTTGAGTCAAACAGGTTTGCCGGGCAGGGCTGGCAGGTCCATGCCTGCAGG
GGCCCTGCAGAGCCCTGGGGTGGAGACTCCACCCAAAGCCCTGGGAGACCGAGGCAGGGAGAAGAAATTC
AACAGCTCCACGTCTGGGCCACAGAGGGCACTTGGCCAAGGCTTGGAGGTCCCAGCAGACCTGGGCTCTA
GAGACTCCAGCTCTACACCTCTGTTCCCATTTTCCCCAGGCCTGCCACATCCTCCCTCCCACCTCCTCCT
GGTAGCCCCCCAACACACTTGACTTGCTCCTAATCTGCGGTGCCATCTGCTAACTGGATGGTGAGCAGAA
CAGCCCAGGGAAGGCTGGGGGGGTTTATAAATTGACTTCCCATGGCAGGCCTGGCCTTTGCCTTCAGGTT
GGGGTCTGTGTCTGGAAAGTGAGCAGGTCTTGAACTTACTGTAAAAATCCCAGCCTGTGTTGCTGCACCC
AGGATCAGTCCTTGGAGCCCGAGGCTGTTGACTTTGGTGTGGTCAGGCCCAGAGCTGTGTCCTCCTCGCA
GCTCTCTGCCCTCAGTTTCCCCACCAGCTGAGCAGGGGTGCCCAGGCCACTTCTCAGGAAATTACATTTG
AGAAAGTGCCTAGAAGAGTGGAACACATGCTGTGTTCGTGAAGGCTTGTGATCACTGTTAAGAAAATACG
AGCGGCCGGGCGCGGGGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGTGGGTGGATCACTTG
AGGTCAGGAGTTCAAGACCAGCCTGGCTATCATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAG
GGCGCCTATAGTGGTGGTACATGCCTGTAGTCCCAGCTACTTGGGAGGCTAAGGCAGGAGAATTGCTTGA
ACCCAGAAGGTGGAGGTTGCAGTGAGCCGAGATCATGCCACTGCACTCCAACCTGGGTGACAGAGCAAGA
CTCTGTCTCAAAAAAAAAATAAAATAAAAATAAAAAATAATTTTAAAAAAGCAAGAAAATATGAGCAGAT
ATTTATTTCTTAGAACGGAAGGAGAAAATTTGGGGTGTATCCTATTTATGGTTGAGGAACCAGACAAATC
AATTGGTGCATAATGATTGAACACGCTGTGAGGGCTGGCGGGGAGGGGAGTCCTGGTCTAGGATGTCAAT
GTGGATTAATTTATTTCACCAACATCCCTGACAGATGAGGGGCCGGGGTGTGAGAGCACAGCCCCCTGCC
CAGCTGGCCGCCTGGAAGTGAACCCGGGCAGGCCGACTCCAGAGCCCACCACCCTGCCCCCACCCTTACC
ACCCTCACAGCAAACCCTGGCCAAGTGGCCTCTGTGGCGCAGGCGTGGAGCTGTTGAATTTCTTCTCTCT
GCCTCCATTTCCCAGGGCCTTGGGTGGAGTCTCCGACCCGTGATCTTGGTTGTATTCATCCCTCTCTGGG
GGTCCTCAGTCATGAGGCTCCTTCCTCTCTGTGCTGGAACAGGGGCTCTGAGGGGCCAAGGTCAGAGGTT
GCTGCCCCCAGCCTCAGCCTTAGACAAGGAGGACAGGCCTGGAAGGAGGCCCAGGGGCTGCCTAATGTCC
TCCGTCCCTCCCTCCCTCTCTCCCTCCCTCCCTCCCTCCCTCTCTCCCTCCCTCCTTCAGGACCCTGCTA
TAGCCATCCCCAAGGGGACCCTCATGGCCATTTTCTGGACGACCATTTCCTACCTGGCCATCTCAGCCAC
CATTGGTAAGTGGCCGGCCCAGCCAGTCAGGAGGGGGAGGGACCTGGCCTCCACCCTGCAGTGTCCCAAA
ACCCTGACCACTGGAGGGGATCAGAGGGTGGGGTTCGACTCTCTAACAACAGGGAGAGCTGGGCTGGGAG
GGTGTGGCGACCTTAGGATGTGAAGCCTTGAACCTATCTCTGGGGTATCTTGGCAATGATCAGAGCATCT
TCATGGCAGTGGGCGTGGTGGGGTCAGACCAGGCTCCTTGGGTCCCTTGAAGGCCCAGATTCATGAGTGT
GAAGATCCTACAGGACAAATCTAGGAGTGATGATGATGATGATGATGATGATAATGATACCAGTAACTGA
CTTCCCATGTGCCCCGCCCTTTGCATGCCAGTCCTATCATTGTCATTTTCCCTATTTCACCAAGGAGAAA
ATGGGGGCTCAGAGACGTTAAGTGACCTGCCCAGGGTCACACAGCCACTGAATGGTGGAGCCAGGCTTTG
AGCCCAGGTCTGGGCTGTTAACACCTGCCTTGAACTGAGATCCTCACAGATGTACTTCCTCCCAGTGGCC
CCGTTTGAGGCAAGCTGTTCACAAATGGCCAAGGGACTTGGTCCAGCTCACAGCCACTCGAGGCTTGAGA
TCCAGGTCTCCGATCTAAGGAGGACATTGTGATCTTGGTCACTCAAGCTCTCTGCGCCTCTGTAAAATGG
CAACGACAACAAAAATAATAGTAATGCCTGCTCGTAGGTTATTGTAAAGTACTTATCATGGCTCCGGGCA
CACCTTCATTGTCATCATTATCATTGCATAATGAAGGGACAGCTGCCCATCATCTGCAGCACCTCGCCCT
CTGCAGAGGTGGGGCTGGAAGAGGACAGAGTAAGGAGGGAAGGCAGACCTCCCCATGCTCTCCTTCCTCC
TCTCAGGCTCCTGCGTGGTGCGTGATGCCTCTGGGGTCCTGAATGACACAGTGACCCCTGGCTGGGGTGC
CTGCGAGGGGCTGGCCTGCAGCTATGGCTGGAACTTCACCGAGTGCACCCAGCAGCACAGCTGCCACTAC
GGCCTCATCAACTATTACCAGGTACTGCCAGGAGAGCTGACCCACCAGACACAGTGGGGGCTGGGTGGAG
GCTGCAGGGCCCCTTGCAGGCCTGGAAGTTTGTTGGGGGGACATAGTTTTGGGGGTTGAGGCCTAGGCTT
AGGAGAGAGGGGTTCAGAGCCCAGGTCTGTCCAGTCCCGACTTGTGGGTGGACACTGGGATCTCCAGGGG
TGGGTTGTGGACTCAGGTGGAGGCTCAGGACCCAGCCCCTGCCCGCAGTAGGGAATGAAGTGCCACAGAT
GGGGGCTCCTGGCTCAGCCCCCACCGTGGAGTCCCTGAGCCCCAAATCCCCACAGACCATGAGCATGGTG
TCAGGCTTCGCGCCCCTGATCACGGCTGGCATCTTCGGGGCCACCCTCTCCTCTGCCCTGGCCTGCCTTG
TCTCTGCTGCCAAAGTCTTCCAGGTGAGGCCGCAGAAAGGGGTCGAGATGACAGGGGGTGGGGAGCCTGG
GCTAGAGGCACAATAGGGCGGGTCCCTGGGTGACTGCTACAAACACCTGAAGGTGTCATTAGACTGGGGT
CTCTAGCCAGAAGGGAAAAGGAGCTATCAGCAAACTCTTGAAGGAAGCAGCCCCCTCCGACTTGACAAAC
ACCTGCAGTCCCCACCCCAAACTCCCACCTGTGCCACCAGCATCCTGTCCGCAACTCCACCATTCAAGCT
CTACCCGAAGCCATCCATCCCCCAGTCATGCTCTTTTACTGTCTTTATGGTAGGGCGGGGAGGGTGGAGG
GGGTGAGCATCCCTGTTTAATAGAAACAGACACCAGGACCCAGGTTGGGGAGGTCACGGAGGGCACCGAG
CCGGGGCTGGAGCTCAGGTGGCCCCAGGGGAGGGGAAGTGGCAGGTCCCAGCCTAAGGGTGAGTGCGGCA
TCTGGTGCTGCAGTGCCTTTGCGAGGACCAGCTGTACCCACTGATCGGCTTCTTCGGCAAAGGCTATGGC
AAGAACAAGGAGCCCGTGCGTGGCTACCTGCTGGCCTACGCCATCGCTGTGGCCTTCATCATCATCGGTA
AGGCTCTGCCAGGGCTCACAGGGCCTGGCCTCCTGTTCCCCTGGCCGGCCATGAGGGTCTTGGGGGCCGG
GCTCTTCTCCTGTCTCCTCATCTCCCCGCCGGGCCTGACAGTTAGTGGGCACTAAGAGGCTAAGTCTTGA
GGGGTGAGACCCCACCTGGGAGGAGACATCGGGGGGCCATGTGATCCTTCCCCTTCCCACCTCCAGGCAG
GGTGGCTCCCAAACCATGTCAGGCAGCAGAGCCTTCTGCTTTGTTAGAAGAGAACTTACCTGTTCCCCCG
GTCTGCGTTCACATGTCCTGGCAGCTAGCATCCCCCTCCCCAGGCCTAAATGAACCCCAGAACACCTTTG
GAGCATGGGACAAGCTGACTGGTCTCTCCTTGGGATGGGATACCAATGGCTGGTCAGTATCCTCATAGGT
ATATAAACTAGACAAACCTAGTTCCAAGGGCCTAGGGCTTCTAGACTCCTTGGGCTTCAAGGTGGTAGAT
GTCTTAAGAGAAAAGAGCCCAGGTAATCGGTTCATTAGATCAACTTCCTATCCAGGCTTTGCCATGTGCC
AGCTGTGTGACCTTAGACAAGGTACTTCACCTCTCTGAGCCTCATTTTTCTCATCTGCAGAATGGGAGCA
ATAGTGCCTAACAGGTGTGTCTCAGATGGGCAGGAAGAGACCACCTGTGCAGGAAGACCTACATGCATTC
ATATATGCACACACACTCATATGTGCACACATACTCACATGTGCACATGCCCAATACTTGCCTAATTTAC
TTAGCCATGGAGGCAGAACTCGCGAGGAGAGAGGTGGCCACACCAAGCACGGGCAGTGGCCATGCTGCAA
CATGATGTCCCCTGGCCCCTGCTCCCTATGCAGGAGTGGGATGGGGGTAGACTACTGGCCAGCCTCCCTG
GGCTCATGGGCACCCTCCTGCCAACAGTGAATCCATGCCGTTCAGGCTGGTATCACAGTCCATGAGATTT
GCCCTGATAGCCTAGTCACTATTACCCAAGGTAAGCTTTTCAGACACCAGGCACTACTGGGTCCTGGACC
ACATGAAGAGGTGGCAGGGATGGAAAAGTCTGGTCAGACTTGATGTTCCCCGGCCCCGTCTCTGCTTCCT
GCTCACCGGGGCCCAGAAACACATTTCCCTGCAATGTACCCTGATGCAGATTCGATGTCCTTTCATCTGG
GGGGGGGTCTGACTTGACTTCAGACTGAGGACCATGATCTCAGATGCTCCGGGCAGGGTTCTTAGGTGAT
TACATGAAATCATGGACAGAGGACCTGGCACATTGGTGCACGTCGTGTTTTGGGATCCCCAGAAGCAGGC
CCCGAGACAAGGATTAATATGTAAAAGTTTATCTGGGGTGCTGGGGGCAGTAGCAGGGGAGGGGAACAGG
GAGACAGGGAAGAGGAAGCAGTTCTTGGGTGGGTTACCACTGGGGTACCAGTGGGGTGGAGCTGTGTTAC
GGTCTTAGGTGTCCTGGGCCACATTGCAAAATGTATTTCTGGCCAGGCACAATGGCTCATGCCTGTAATC
CCAGCACTTTAGGAGGCCGAGGCAGGTGGATCATTTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACAT
GGTGAAACCCTGTTTCTACTAAAAATACAAAAATTAGCTGGGCGTGGTGGTGCATGCCTGTAATCCAAGC
TACTAGGGAGGCTGAGGCAGGAGAATCGCTTGAGCCCAGGAAGCAGAGGTTGCAGTGAGCCGAGATCACG
CCACTGCACTCCAGCCTGGGTGACAGAGCGAGAGTCCGTCTCAAAAAAAAAAAAAAAGAAAAGTATTTCT
TGCTGTCATTTGTGGTCAAAACAGTGTGACAGCCACTACCCTTGGTCATCCCATAGCCTGATGGGGTCTA
CTCTCACACTGACCCCCACTGTCTTAAAAGCTGGGCGCTTACAGTTTACAAGACACCATCCCTTTGAGCT
TCACAAAAGGATGGACCCATCTCACAGATGAGGTGGACCCATTTCACAGATGAGAAGGTTGAGACTGACT
GAGCCTTGGTGGCCTGTCTGGGGTCCCCCACCCTGGGAAGGAGGGTGCCAAGCCAGTCCTTGGCAGAGTT
GCCCAACAGGCTGTCCTCTCTCTCCCTGGGTCCCCGAAGCTGAGCTCAACACCATAGCCCCCATCATTTC
CAACTTCTTCCTCTGCTCCTATGCCCTCATCAACTTCAGCTGCTTCCACGCCTCCATCACCAACTCGCCT
GGTAAGCAAACCCTTCACCCACCTCAGGAGGAGGCACCCAGGGGGCAGGAGGAACTGGGGCATGGGGTGG
GAGTGGGAGGCATGGGTGGAGGTTGGCAGCCCAAGGTCACCTCTCAATTTAAAACCATTGAAAGGGAACA
TTAATATAATAATAGCAGCTCTCGGCTGGGCTCGGTGGCTCATGCCTGTAATCCTAGCATTTTTGGGAGG
CGGAGGTGGGCGGATCACTTGAGGTCAGGAGTTTGTGACCAGCCTGGCCAACATGGTGAAACCCTGTCTC
TACAAAAACACAAAAATTAGCCAGATGTGGTAGCGGTCACCTGTAATCCAAGCTATTCGGGAGGCTGAGG
CAGGAGAATCACTTGAACTGGGAAAGGGGAGGTTACAGTGAGCAGAGATCGCGCCATTGCACTCCAGCCT
GGGTGACAGAGCAAGACTCTGTCTCAAATAATAGTAATAATAATAATGATAATAATAATAGCGGCTCTCC
TTTCTCGCGCACTGACAGTGTGCACTTTACATGGATTCATACATTGCTTACCATCTGTAGGGGTTTTCCA
GTCTTTTCCTTCCCCAGTTTTACAGGAATCAGGAGCCTGGTTCTTCAGAGAAAGAGAATTGCCCAAAGTC
ACAGCTAGGGCTGTCACAGACAGTGCTGCAGGTTGCACACTGCATAACTCTAGGAGGCACCATTCATATC
ATAGATTTCATATATTTGCATGTTTATGATGGAAAACTTCTGGCAGATGGCAATAAAGAGGCTTGAGGAA
GACTTTTTCTTTCTTTTTTTTTTTTTTTTTTTGAGACGGAGTCTGGCTCTGTTGTCCAGGCTGGAGAGCA
GTTGCACGATCTCGGCTCACTGCAAGCTCCACCTCCCGGGTTCATGCCATTCTCCCACCTCAGCCTCCCC
AGTAGCTGGGATTACAGGCGCCCACCACCACGCCCAGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTT
TCAGCATGTTAGCCAGGATGGTCTCGATCTCCTGACCTCATGATCCGCCCGCCTCAGCCTCCCAAAGTGC
TGGGATTACAGTTGTGCCTGGCTGAGGAAGACTTTTTCTAACCAGCTCCAAATTGCCATTGTCTTTTACT
AGGCCACCTTTTACATAGATCCCAATGTGTATGATGGCCCTGGAGTTGCACCATGAGGCAGCTGTCACTA
GTGGCAGCCCTGGCCTGACCTGCTGGTGGGGAGCCAGGCAGAATCATGTCCTTCCCCCTCCATCTCACCC
CCTTGGCACCATGGGAGCTGGTGCTGTTGCTGACATGGGATGTCCTGTGGCTGTATTTGGCCAGAGCTGG
ATGCTCCTGGTGAAATGCCAGCCAAAGCAGGCGTGTGATTTGCCCCAGAGCCTGTAGACCCAGCCCAGGA
GGCAGGGTGGGTGGTGCTCATGGCTGGTGGGTACAGGCTGGGACCCCGGCTCTGGGCACTGCTGGCATTA
CTGCCAGGCCCTGCCCAGCAGCTCTGGCCTAGAAAGAGGCTCGACTGCCAGGCATGCCCACTGACTGGTG
CCCTTGGCCCAGGGTGGAGACCTTCATTCCAATACTACAACAAGTGGGCGGCGCTGTTTGGGGCTATCAT
CTCCGTGGTCATCATGTTCCTCCTCACCTGGTGGGCGGCCCTCATCGCCATTGGCGTGGTGCTCTTCCTC
CTGCTCTATGTCATCTACAAGAAGCCAGGTGCGCATCTCAGCTGCGGGGCCTCGGCCCTCCTCCCCCAGG
GTAGCCATGCAGGCGGCCCTGCCCTCCGCCCCAGTTGGAGGGCCCTGAGTCCGGCTCTGTGCTGTCCAGG
GCCCCTCTCTGCCCCTCCCCTTTATCCCTCCCAATGTGGCAAGAAACCCCAGGGGATGTCCCCACTCAGG
GCCTATGTCTCGACCTCTAAGCCACCCCTGGGCACCCAGGACCCTGGAATCCCCTGTCCGAAGGACCCTG
AGTGAGCTTCCAGGGCCTGCTTCAGCCTCTTCCCTAGGGCTGTCCCCCAAGAGGGGCCAGCAGGCTGCTG
TGCACACCTAGGCCTCAGAGGTGGCTGAGGGCTGGTTGTGTGGATGGCAGGCAGACAAAGCTTGGACTCC
GGGCAAGGTCTCTACTTGGCTGCAGGCAGGGACCCTGGGGTGGCAGCGACCTGAGGGCAGGAAACAGGAC
TCTAAGAAAGCCTGGGATTCTAAGTGTCAACCTGGGCTTCTAGGCCATTGCAAAGGTTTGGCAAGGCAGG
AGGATATCGTGTGGAGCTTGAGGTGTAACTTTTTTGTGGGGGGACTGGGGTGCAGTGTCTCATTCTGTCA
CCCAGGCTGGAGTGCAGCAGCACAACCTCGGCTCACTGCAACCTCCACCTCCCGGGTTCAAGCAATTCTC
CTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGTGCATGCTACCATACCTGGCTAACTTTTGTATTTTT
AGTAGAGACAGGGTTTCACCATGTCGGCCAGGCTGGTCTTGAACTCCTGACCTCAGGTGATTCGCCTGCC
TCGGCCTCCCAAAGTGCTAGGATTACAGGTGTGAGCCACCGTGCCTGGCCTTGATGTGTAACTTTTCAAC
GTGCAGCCACACAGTCCGCAGGCCTCCACCTTGCAGGCTGCCCATCCCAGGTGGCCCTGCTCCCACGGGT
GCCCGGTGCCTAGAGAAGGCCGACATTACCTCTGTCCCTCCACGTGTCTGGTTTCCTCTAGTGATTCCTA
ACTCTGCTCTCACCCCCGTTGCTCCCTTGCTCTCCCAGAGGTAAATTGGGGCTCCTCGGTACAGGCTGGC
TCCTACAACCTGGCCCTCAGCTACTCGGTGGGCCTCAATGAGGTGGAAGACCACATCAAGAACTACCGGT
GAGCAGAGCTGCTGGGACCCACCTGGGACCCCAGGGCCAGTGATGGCTCCACCCTGGGAGTTTCCAAGCC
TAGACCTGTCACCTGACCCAGGCAGACCCAGGGTATGTGCAGCCTCCACTCAGAGAGGGAACAGACATGG
AGGAGCCACCCAGCCACCACAAGACAGGGGTGGACATATCCTGATGGGGACCAAGCAAGTCAAGGGGCCT
CTCCATGCCTTGGTTTCCACAGTGGAAACGTGCTTTGGTGTTGAGGTGATTGTTGCTGCAGCTCCCATCA
CAGTATCGCCGGAGCCAATGGTGACGAACCTCCCCTGGGTACCCTTCAAAGCTCATTCCCCTCTTTGTAG
AGGAGGTTTATGCACCTGCCATCCTCTTCCCACCAGATCCTAATAATTATTGGCAATATCTATTTATGTC
ATGCTTACTATGAGCCAGGTGCTTGCAAAGTGCCTTACACAGCCTTACAAGAACCGGCTGAGGTCCAGGA
AATTACTAACCCAGTCCCAAGAACACTGAGGCACAGAGAGGTTAAGTAACTGGCCCCAGGTCACACAGCT
GGCAAGTGGCCGATTAAGTCTGGCTCCAGTTGACTCAGGTCATGGCCCTTGACCACTGCCCTCTGCTACC
TCTCGACGCAGAGGCTGAGTCACCTTCCCGGGTAACTGATGAGTTTGTAAGTGACAGGTTTCCTCACTCC
CAGGCTTTGTTCTTTCCCCCACCATGCTGGGCTAGGCAGGGCAGGGGACATGCATGGGGGGTAGCATTTT
GTGGCCTGAGCAGGAAGGACCAGGGAGACTAGTGTGGAGGTCACCAAAAGAGCAGGAAGAGCCCCAAAAG
TCACCCTGGATTTATAGGTGGGAAGCCGAGGCCCCAAGCATGTAGGGTCATGCTGGTGGCCACACCACCA
TTCAGGGAGCCTGGGAGGTGCCTTTCGCACCCAGACCCCCGTGGGCTCTCTCCTGATGGCTCCTGCCCTT
TTCCCTTCCCTCCTCAGCCCCCAGTGCCTGGTGCTCACGGGGCCCCCCAACTTCCGCCCGGCCCTGGTGG
ACTTTGTGGGCACCTTCACCCGGAACCTCAGCCTGATGATCTGTGGCCACGTGCTCATCGTGAGTGGCCC
CTGGAGGGGCACAGGGGACCAGGCATGGTGGCTCATGCCTGTAAACCCAGCACTCTGGGGAGCCAAGACA
GGTGGATCACCCGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACACAGCTCCTGGGGGAGTCGCCTGGGT
GTGATGTCCACCCACAGAGTGGGGGCCGAGGGGATGCGGAGCAAGGGGCACCCAGCCAGGCACCCCCCAT
GGACCCCATTCTCTCCCCTTCCTGGCCCCTCTTGCTGGCTTCTCCCCCAGCTGCCCTGCCTCTTTTCTGC
CCCCTCCCTCTCCCTTCCTCCCCACTTTCTCCCCCACTCCTTGTGTTTTCCCTTATCTGGGCAAAAGAAA
AGGGCACCGTGGGTGCTGCCAAGCCCCTGGGGCAGCCTCCAGGGCAGGAGAGGGGTTGAATCTCAGGCTG
GAGGGTGAAGGCAGCTGGTGATGTCCCCTGCCCCTCCCACCCACAGGGACCCCACAAGCAGAGGATGCCT
GAGCTCCAGCTCATCGCCAACGGGCACACCAAGTGGCTGAACAAGAGGAAGATCAAGGCCTTCTACTCGG
ATGTCATTGCCGAGGACCTCCGCAGAGGCGTCCAGATCCTCATGCAGGTGCCATGGACTGGGGGCTCCCC
TACAGGACTTACGGCTTGGTGGCACAACCTGGAAGGCAGAAAGTCTTGGCGGCCCCTTTGCTTAAGCCCC
TTTTGCTGCAGAAGGAGCCCCAACTTTTTTTTTTTGAGACTTGTTTTACTTGTCACCCAGGCTGGAGTGC
AATGGTGTGATGTCAGCTCACTGCAATAACCTCCGCCTCCCGGGTTCAAGTGATTCTCCTGCCTCAGCCT
CCTGAGTAACTGGGATTACAGGCACCCACCACCACACCCGGCTAGTTTTTTTGTGTTTTTAGTAGAGACG
GGGTTTCACCATGTTGGCCAGGCTGGTCTCTAACTCCTGACCTCAGGTGATCCACCTATCTCAGCCCCCC
AGAGTGCTCGGTTTACAGGCATGAGCCACCATGCCTGGCCAGAGCCCCAACTTTCTTATGTCTCCAGATG
TGCTGGTTCTGGGCACTTTCTCGGAATGCCCAGGACCTGGGAAACTGACTTGGAAGCCTGTGGGGGCTTG
TGTCACCTTGGCCAATTCTAACTCTTGGGGGCACTGGGAGGGATGTAGGGTGTTGGTGACCAAGCGAGAA
CTAGAATCGAGGATTCTGGAATTCAGATATTTCCTCCCATCAGTCATCTTTCTCCCAATAAAGTTTTGTT
TTGTGCAAAACAAAAATTTTTAAGATTATATATATATATATATATATATATATTTTTTTTTTTTTTTTTT
TTGAGAATCAGCACATCTGGAGACATCAGAAAGTTGGGGCTCTGGCCAGGTGGATCACCAACTCTGCCCC
TCTGATGGGTTCCCCATCTCACCCCTATCCCCTGGCAGGCCGCAGGTCTCGGGAGAATGAAGCCCAACAT
TCTGGTGGTTGGGTTCAAGAAGAACTGGCAGTCGGCTCACCCGGCCACAGTGGAAGACTACATTGGCATC
CTCCAGTGAGTCGGGGGAGAGGAAGGGGCTTGGGGTCTGTTAATTTGGGCCCATTGGGCCTTGAGAAGTG
GAAAAGAAGTAGTGGGTGCTTAAAAAGTTGAGTCCTGCTGGGCAAAGTGGCTGGCATCTGTAGTCCCAGC
TACTTGGGAGGCTGAGGCAGGAAGCTCACTTGAGCCCAGGAGTTGAAGGCTGCAGTGAGCAAAGATTGCA
CTGCTGCACTCCAGCCTAGGCGAAAGAGCAAGACCCCATCTTTGAAAAAGAAGTTGAGTCCATTTTCTCA
AAGCTAAAGAAGAAACACGCTGAGGCTGAAGATGCCCGTCTCTGACCTGTGAGTCATGGACACCAAAGCC
AGCTGGGGCCAGGCAGGTAGCACCATGAGTGAAGTGGGCCAGGCCAGGGGTGATAGGGAGCAGTGGAGAC
TGTGGCAACCCAGAGAGAGTGGCTCCTACTTGGCTCGTGTCCATGCAGAATGGGCTCTAGTGCCCGTGCA
GAAATGTGGGCCCAATGTTGCCAGATCTTTTAATTTTTTTTTTTTTTTTTTTTTTTTTTTGAGACGGAGT
CTCACACTGTTGCCCAGGCTGGAGTGCAGTGGCGCTATCTCGGCTCACTGCAAGCTCCGCCTCCCGGGTT
CACGCCATTCTCCTGCCTCAGCCTCTTGAGTAGCTGGGACTACAGGCGCCCGCCACCACGCCCGGCTAAT
TTTTTGTATTTTTAGTAGAGACGGGGTTTCACCGTGTTAGCCAGGATGGTCTCGATCTCCTGACCTCGTG
ATCCGCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTCAGCCACCGTGCCCGGCCACCAGATCT
TTTAATTTTTCAGAGTAACTGCAAATGTGAGTTTTTAGGTGAGAACCTCATGCCTCCCTGTGGGCTCCAG
AAGGTGCACAGGGAACCAGCTCAAGAGCTCTGAGGCGGTGCCCCTGAACAGGGAAGTGAAACCAGGACCT
GAGGACGGAACTCCCTGGGGACTTCAGGGCTGACTCCAAACTCTTCATCAGTGCTTGCTGTGTGATGTCC
AGAGATGCCTCCCTCAGTGGCTTTTCTGTGTTGATGTTGTTGTGTGAGGTATCACTGAACAACATGAGGC
TGAGGGTTGGGGCCCTGAGACCTGCTTCCAGGTATGCTGTTAACCAGCTGTAGGACTCGGAGCAATCCTC
TCAGCAGGTGCTAACAGAATTACAGCAGCAACTGATAGAATACCTGATCTCCAGCCTCCCTCCAGCTCCA
ACATTCCAAACCTTCAAAAAGCAGGGCCTGAAAGTTCCTTGTCCTGGAGATATGTGATCTAAGGAGCCCA
GGGAGGGCAGTGCGCAGCCCATTGGCCCAAACCCCAGTGGTGGGCGGGCTCTTGGGCACTGTGTCAATCA
GGCAGTACATACACCTTTGGAACAAGAGTCTTCCAGACATCTTTAATTATTTTATTTTATTTATTTTTGA
GACAGAGTCTTGTTCTGTCGCCCCGGCTGGAGTGCAATGGTGCGATCTTGGCTCACCACAACCTCTGCCT
CCCAGGTTCAAGCAATTCTCCCTCAGTCTCCCAAGTAGCTGTGATTACAGGTGCCCACCACCACGCCCGG
CTAATTTTTGTACGTTTTAGTAGAAATGGGGTTTTGCCATGTTGGCCAGGCTGGTCTCGAACTCCTGACC
TCAGGTGATCCGCCTGCCTTGACCTCCCAAAGTGCTGGGATTACAGGCGTAAACCACTGCGCCCGGCCTC
CAGACATCTTTATAGACAGCCTATTGCAGGAGTAAAGCTCCTCTCATGAGCATGTCTGTCCTTTGGTCCA
CAGTCTCAGCCATGTGATTCTAATCCCAGCTGCTCACTCCCTTAGCTGTGTGACCATGGGCAAGTTACTC
TCCAAAACTCAGTTTTTGCATCCATAGAATGGAGGGAATAATTACCATCCCCACCTCCATGGGCAGAGTT
GAGTTGTGGGGGATTATCCAAATGTATCAGGAGGACAGGGCTGACTTGGTTCCCCTGAGTACCGGATCTG
CAGGATCCACAGTGCCACAGACTGTAGAGCTGGAGGAGTCCTGGGGTACTCTCCAATTCTGCCTGTACAG
GATACAGATGGGGAAACTGAGGCCCAGAGAACAGAAAGGGCGTGGTAGGAAGCAGAGCCAACTCTAGATA
TCTGGTCTCCTGGGTGCCAGGTCACCTCCCCAGTGGGAGCTGGGGGAGAAGCTGGACCTCACCTCCTCTC
TTTCCAGTGATGCCTTTGATTTCAACTATGGCGTGTGTGTCATGAGGATGCGGGAGGGACTCAACGTGTC
CAAGATGATGCAGGCGCACAGTGAGTACATGCCCCACCCACTCCCAGAAAGTTCTAGAACACATTTTTTG
TTTTTAAATGGCAGAGGGAAAAATGAGTAAGAAATAAAAGGTTACAGACTCATAGAAAGTCGCCTCTTAA
TGGTCCCTTAGAGCCCATCAGTTTTCCCAGAAAGAGAAAGCAACGCATTCAAGGTCATGCTTCCCAGGTG
GGGCAGAGCCAGGACTGGAACTCAGGGTTCCCCAAATCACAATTGGGTCGGGGCAAAAGGATCTAATATA
GGCCGGACATGGTGGCTCACGCCTGTAATCCCAGCGCTTTGGGAGGCCAAGGCAGGTGGATCACCTGAGG
TCAGGAGTTCAAGACCAGCCTGGCCAACATGGTGAAACCCTGTTGCTACTAAAAATACAAAAATTAGCTG
GGTGTAAGGGTGGTGCACACCTATAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCACTTGAACCC
AGGAGGTGGATGTTGCAGTGAGCCAAGATCGCGCCATTGCACTCCATCCTGGGTGACAAGAGCAAGACTC
CATCTCAAAAAAAAAAAAAAAGATGTAATAGAAAACCAGGAAAGAGGGGAGAGGAAAATGCCTGCAGGAA
GATGCCAGAATATGAAAATACATAATGACCATGGGAACCGGGCATTTTGGTCCACTGAGTAGGGATGTGT
TTTGCATTTTAAAGTCATATATGACTGCAGACTCTGAATTAGAAATATTTAGAGAGGCTGGGTGTGGTGG
CTCGTTTCTGTAATCCCAGCACTTTGGGAGGCTGAGGCAGGTGGATCGCTTGAGTCTAGGAGTTCAACAC
CAGCCTGGGTAACATAGCAAGACCCCATCTCTACTAAAACAAAAAATTAACCAGGTGTGGTGGCACGTGT
CTGGAGTCTCAGCTACTTGGGAAGCTGAGATGGGAGGATCGCTTGAGGCCAGGAGTTTGAGGCTGCAGTG
AGCCGTGATTGCACCACTGCACTCCAGCCTGGCTGACAGAGCAAGACCCTGTCTCAAAAAAAAAAAAAAA
AAAAAAAGGAAATATCTAGAGGTGTGAAAGCAATAACGTGTATGGCAACAAATGAGAGGCAGTGCTGGAG
AGACTGGGAGCTTGGAGTCCGCGTCTCTTGCAGCCAGGAGCTGGGTCCGTTCATTTGTGCCATTGCTGAG
GGCATGTCTGGCACACGGATGCCACCAATATTTACAGAGCAGTAACTCCACTCCTGACCAACGCTGCTCT
TGGTGCTGGGGTTACAGGCACACCAAAAGCCAAAAAGCCTCTGCTCTTGAGAAGCTGACGCTCCAGAGGG
AAAAGCAGACACTTGCCAGTGGACCCATAAGTTATTTCAGATTGTGAGAGTGTCTGGAGAAAACGGGTGC
AGGGCGTAGTGGCCAGGGAAGGCCTCCCTCAGGAGGGGGCCTTTTGCTGAGGAGGCCAGGGCTAGGGGAA
GTATGTTCCAGCTCAGGGCGCACCCACCGCCACGGCCCAAGGTGGGGATGAACGTGGCCTGTTTGAGGGG
CAAGAGGAGGCCATGCGCGACTGGAATGTGAGGAGCCGGGCAGGAGGCAGGTCAGAGGGAGGCCAAGGCC
AGGTGCAGTGGGGCTGTGGAGGCCAGGTGGGGCATCGGGACTTTCTTCCTAGCATTAAGGGAGCCCTGTC
AAGGAGGAACCCACGGTGCCCTCAGACAAGGAGAGCCTGTGAACAAAGCTGCCTCTTCTTTTGCAGTTAA
CCCCGTGTTTGACCCAGCGGAGGACGGGAAGGAAGCCAGCGCCAGAGGTGCCAGGCCATCAGTCTCTGGC
GCTTGTCAGTGTTCCCACTCAGAGCTCAGGGCACTCTAGCCCTGTGGGGTGGCTAGGGGTGGGCCACTTG
GGAACTTGGTCGAGGACAGGTGGTTTTTTCTTGTGACGGTGGTGCCTGAGTAACTGGAGTTGGGAACTGG
AGGGGCTTGGCCGTGTCAAAGATTGCCAGCAGCCAGGCGCAGTGGCTCATGCCTGTAATCCCAGCACTGT
GGGAGGCTGAGGCAGGAGTTCCAGGCTGCAGTGAGCTGTGACCGCACCACTACATTGCCAGCCTGGGTGA
CAGAGTGAGACCCTGTCTCAAAAAAAGAAAAAATATTGCCAGGAGGTGGAGAATTCAGGGTCACGGCATC
ATGGGGACCCACCTAGCAGGCTCTGGGGCCCTTGTCCCACCCCTGCCAAGGCCCCTCTAGCCATGCAGGG
TGGGGTGGGATTTTCCTTCGTCCTCTCCCACCATCACTCCGGGAGACAGGGGGCCTGAAGTGCAGGCGGG
AGGGCTGCTGTGGGCATTTGTGAAGGGTGCAGGAGGTGTTTAAGGGAATGGAGAGTGCACTTCCCTACCT
ACCCAGAGGGCTCTGGGACTGCCCCCACCCCCAGTACTCACCAGAATCCCGGGAGAGAGAAGCTGAAAGC
AGGGCAGGGGCACATCACAGCACTGAGCGTCCTGGGCCCCGGTTCCTGTTCCACCTGCCAGAGAACCCGT
GTTCAACATCAGAAGGGGCGTTGGCGGGGCCCTGGGCCAGGCCTGCCTGGATGCGCGGCTGCTGGCTCTG
CTCTGACCCGCCCCCACCTCCTGCAGTGGACCCCAAGGCCCTGGTGAAGGAGGAGCAGGCCACCACCATC
TTCCAGTCGGAGCAGGGCAAGAAGACCATAGACATCTACTGGCTCTTTGACGATGGAGGTCAGTGACCCC
CTTGGATCAGCCCTCCTGCCCGGCGGGGGCGGGGTGGTGGTGGTCTTCCTTCCTTCTCCTTCCTGGCCTG
CTCTCAAAGGGGACAGGGGCTCCTGGGCCCAGCAGTGAGCTCAGGGGAGCCCAGAGGGACCCCTCTGTCT
GGCCTCTAGTTTTTTGGTTATAAAATGGGAAGCTTTGAGAGAAGAGCTGAAGTCGCCATGAGATGGAAAC
TTCTGACTGGATGGCCCTTAGGGGCACAGGCAGGTGATATAAATGGGGAGATGGGCGGTGCCCAGGAAAG
CTGGAGGAATATTTTTCACTTCACAATGAAATATAACCTCTCCCATTAAAAAACAAAAAAAGTGACTTTA
AGCTTTATTGCTCCTACCTTTGATAGAGTCACAGATAGAAAGAAAATGTCTTCGCTCCAATGGAACGAGT
CCTATTAATAACTGCCAAAATAGGCTGAGCACTTACTACCTGGCTGACAGGGAATTCCCTACCTAATCCA
GTCCCCAGGTAGGAGCTGTCATGATTAACTCCGCTTTATAAATAAGGAAAATGAGGCACAGAGGATTACA
AGGTGATATGTGACAGCTGGCCCTTGGTCTTGTGTGGAGAGACAACAGAGAGGGATGAGGGCTCCCACAC
GCTGGTCAAATATTCACTCTTCAAGAACTGCCAATGACCTCGATTTTTATGTGAAACTCCTCAATTATTA
AATGACTTTTGTTTTTTATTATTCTTTTTGAGACGGAGTCTTACTTTGTCGCCCAGGCTGAAGTGCAGTG
GCATGATGTCGGCTCACTGCAACCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGT
AGCTGGGACTACAGGCGGCTGCCACCACACCCAGCTAATTTTTGTATTTTTATTTTTATTTTTATTTTAT
TTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTTATTGAGATGGAGTCTCACTCTGTTGCCC
AGGCTGGAGTGCAATGGTGTGATCTCGGCTCACTGCAACCTCTGCCTCCTGAGCTCAAGTGATTCTCCTG
CCTCAGCCTCCTGAGTAGCTGGGATTATAGGTGTGCACCATCAGGCCCAGCTAATTTTTTGTGTTATTAG
TAGAGATAGGGTTTTACCATGTTGGCCAGGTTGGTCTTGAACTCCTGACCTCAGGTGATCCACCTGCCTC
GGCCTCCCATAGTGCTGGGATTATAGGCATGAGCCATGGCGCCCGGCCGCCAGCTTGTTTATTTAAATAT
GCTGCACAAGCACAAATAAACCCTACCCCACAGAGCACCGTGTGCGACTTGAATTCAGTCAGCCATGTTC
ATTATACAGATGGGTCGACTGGGGTCCAGCGAGGAGAGGAAGGGGTTTGCTAATGGCAGAGCGGGGCAGG
AACTCACATAGTGCTCTGTCCTGAGTGTATTCTTGTCATGACTCACGGGGACTCTCCTTGCCAGGCCTCA
CCCTCCTCATTCCCTATCTCCTTGGCCGCAAGAGGAGGTGGAGCAAATGCAAGATCCGTGTGTTCGTAGG
CGGCCAGATTAACAGGATGGACCAGGAGAGAAAGGCGTAAGTGTGGAGGGCTGGCCTGGGGGTGACTGCA
GGGACCAGTGTCATCTTAGCTCCACCCAAGGCTGTCCCCTACCCTAGATCCTCTACCACCACCCCCAGGT
GGGCACTGAGATTGTCCCAAAGCCCAGGGCCAGCTCTCTCCAGGCCATGGCAGTGGGCAGGAGCAGGAGC
AGGTTTCTCTCCATTCCAGAAGTTGTTCTGGTTTCCTGCCCCCTTCAATAAATGCAGATGCCCTAGGCTT
TGCTACGACAGGGAGGGGAGTTAGGTTGTAAAGTCACAAATCTCTGGGTTTGGATAACAAGAAGCATAAA
AATAAGAAAGAAAAGGAATCCATTTTTTAAATTTAAAGTTAAGAAAAGACAAGAAAAATAGAAAAAAAAA
AAGATAAAAAGAAAAACAAAAAACAAATCTCCTCCAGGAGGAACCTGTATGAAATGAAGGATGGGCTGGG
CCAAGGCAGCTGGATCCAGGAGTCCAGGAGTTCGAGACCAACCTGGGTGACATGGCAAAATCCTGTCTCT
ATGTTTTAAATTTTTATATATTTTAATTTTTTTTTTTTTTTTTGAGATGGATTCTCACTCTGTCACCCAG
GCTAGAGTGCAGTGACGCAATCTCGGCTCATTGCAACCTCCGCCCCCTGGGTTCAAGCAATTCTCCTGCC
TCAGCCTCCCTAGTAACCAGGATTAGAGGCGTGCGCTACCATGCCCAGATAGTTTTTTTGTATTTTTAGT
AGAGATGGGGTTTCGCCATGTTGGCTAGGCTGGTCTCGAACTCCTGACCTCAAGTGATCCACCCACCTCA
GCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGCCCCCAGCCAATATTTTAAATTTTTATATAAA
TATTTTATATATATTTAAAAATTTATATATAATTTTTTGAAAAAAGGAAATGAAGGCTGGAGAACACGCC
TCCTCCTGAGAAATGAAGTAGGGGCTAGAATCCTTTAGGGCATCAGTCCCCAACCTTTTTGGCACCAGGG
ACTGGTTTCGTGGAAGACAATTTTTCCACAGGCTTGGGGGAGAGTGGTGGTTTTGGGATGGTTCAAGCTC
ATTACATTTATTGTGCACTTTATTTCTATTATGATTACATGGTAACATATAATTTAAAAATGATACAACT
CACCATAATGTAGAATCAGTGGGAGCCCCGAGCTTGTTTTCCTGCAACTAGATGGTTCCATTTGGGAGTG
ATGGGAGACAGTGACAGATCATCAGGAGGGTTCATGGCCCTCTAAGATCCTGCAAGAGGGTTTGTGGCCC
TCTAAGAGTCTAATGCCACTGCTGATCTGACAGGAGGCAGAGGTCAGATGGTAATGAAAGCGATGAGGAG
CTGCTGTAAATACAGATAAAGCTGCATTCACTGCCATGCACCCGGTTCCTAACAGGCCATGGTCAGTACC
TGTTGGCAGCCCCGGGGGTCAGGGGCCCCTGCTTTAAGGCAGTGTCTCCAATGCATGGGATGCAGACTCT
GGGTGAGACAAGAGGTGACTTTAGTGGCCAGGGGTTGAGGCAGCAAATACCACTGAGTCTTTTGGTGAGA
AGTGCCTTTTAATTCTCCTCCAATTCTGGGGCTGATTCTGCAGAAAGTCCTGGTTTAGCCTTGGCCCGTC
CTTCCCTTTGGTAACCTGTGCTTGTCTCCCGGAGCAAGAGGAGTAGGCTGGATGCATGGGCTTGGCAGGC
AATGGCATCTGGCCAGAACTTAGTGACCCTGGTTTATCGACATGGGTTTTATTGGACACCTCTCCCTGGC
AAGAGGTGCTGGTTTTCTGTTTAAAATTGTGATGTTGGCTGGGCACAGTGGCTCTGCCTATAGATCTTGG
CACTCTGGCAGCAGGCCAAGGCAGGGGATTGCTTGAGGCCAAGGGTTTGAGACCAGGCTGGGCAACATAG
TGAGAGCCCATCTCTACCAAAAAACTTAAAAAAAAAATTAACTGTGAATGGTGGCATGTGCCTGTAGTAC
TGGCCACTTGAGGCTGAGGCAGGAGGATCACTGAGCCCAGATCAAGGCTGCAGTGAGCTATGATTGCACC
ACTGCACTCTAGCCTGGGTGAAAGAACAGATGCTATCTCTATTTTTTAAATAATAATATTTTATCATGAT
ATAAAATTTCCCTTTGAAATGTATCTAAGTTTGGTCCAGGCGTGGTGGCTTATGCTTGCAATCTCAGCAC
TTTGGGAGACCAAGGTGGGTGAATCAGCTGAGGTCAGGAGCTCAAAACCAGCCTGACCAATATGGTGAAA
CCCCACCTCTACTAAAAATACAAAAATTAGCTTGGCATGGTAGCATGTGCCTGCAGTCCCAACTACTCAG
GAGGCTGAGACAGGAGGATTATTTACACCTGGGAGGCGAAGGTTGCAGTGAGCCGAGATTGTGCCACTGT
ACTCCAGCCTGGGCAACTGAGCAAGACTCCATCTCAAAAAAAAAAAAAGTATCTAATTCAGAATGTGAGT
TGGTTTAAAGAGAAAAGGAAGTAAATAGTAAGGCAGGTGGCACACAAGTATGGAGTTGACAGATGTCGGC
AGGTGGAATTGGGCAGCTGCTGCTTTGAGCAAGTTCAGGTGAGGTGGGAGGTGGTAGGCTGGAGGTTGGC
TGGAGGATACAGACAGATGGGGCAGTTTGCCCAAAGTCATCCAATTAATTAGCCAAAGAGAAGAACCCCT
GCCCGGTCTCTAGTGCCCTTTGCAACTATCTTGGCTGTGATGCCCCAGGGTCAGAGTAGGAGGGGATCCC
CCAGCCTACACCACCCGGTCCTATGCCAGGATGGCCTCCCGTGCTCCATTCTCTATCACCCGCAGTGTGA
GTTAAGGGAAACCCAGCAATCCTATCACTTTGTCCTCACCCCCACTAAGAACTTTCTCCTGGGAAAGCCC
CTTTTCTGCAATGCTAAGGAAAGTGTTGCTTGGGAAAAGCAGCATCTGCCTTCTCTGTGATCTCCAAAAA
GTAAATAAGATACGAGATTGCTCTGCAGTCTCTGCTCCCTGTCCTGGCTCTGAATGGAAGCTGGGTTCAG
TAGGTTTGGTATAGCTCAAGGCTATGGAAGCCTGGGCTAACAGCATGTCCTTGGTGGGAAGGTTAGGTAA
CCCTTCCTCTGCCTCCCTCTGTCCCTTGGTGGGCCGCATCCTCTGCTCTCTACATAATCCTGGGGCAAGT
CTATGCTGCCCTTGCCTAGGCACTGCCCAGGCCTGCCAGCCTCCGCTGCTACTGCACACCAAGACAAATC
TGCCCACGGCTCTGCTTTCAAAATTTCACTCCCCAAGATCAGGGACCAGCAACGGCTTGCCTGCTGGGTC
CCAGCCTGGTCCTCCAGACTTGTTTCCCATTTCTTCCTCTCTCCCAGCACACTCCATGCATCCCAGCCAG
CTCCTCACTGCCCTGTTCCACCCCCCTGCACCCCGATGCCTTCCCTCCTTTTGACTGTTCCCCACCAACC
AGTCCAGTGGTCCCTAACCAGGCTGGCACATCAGAATCACCTGGGGCATTACCAAAACCATGTCCTTCCC
AGGGGTTCTGACTCAATGATGGGCAAGGGGTTGGGGGGAGGGCCCAGGCAGGTGTATTTTTTTGTTAGTT
TGATTTGGTTTTGTTTTGTTTTGTTTTGTTTTGTTTTTTTGAGATGGAGTCTTGCTCTGTCACCCAGTCT
GGAGTGCAGTCGGCTCACTGCAAGCTCCACCTCCCAGGTTCACACCATTCTCCTGCCTCAGCCTCCCGAG
TAGCTGGGACTACAGGCACCTGCTACCACGGCCGGCTAATTTTTTGTATTTTTAGTGGAGACGGGGTTTC
ACTGTGTTAATCAGGATGGTCTTGATCTCCTGACCTCGTGATCCACCTGCCTCGGCCTCTCAAAGTGCTG
GGATTACAGGCATGAGCCACTGCGCCCGGCCAGGTGTATTGTTTTGAAGGCTTCTTGGGTAATGTTAGTG
TAGACCCTCCCCTGCCCCACCCGCTTTGGCCTGTAACAATCTTACCCCTCTTTCACCGTCCAGCCAGACC
GCCACCTCTGTGAAGTCTTCCCTGACTGCTACAAACCACAGTAGGCTTCTCCTTCCCTGTTAGATGCAAA
GTCAGTGCCATCTGTCTTGCTACTCCTGGGTCTGTACCTAGTTTCTTGTGGGTTATCTTCGCTTCCTCAG
CCGCTCTAGAATTTCCTTTATTCAACTTTCCTATTCCTTAGACCTTTGTTACTCAAAGTGTTGTCTGTGG
ACTAGCAGCATTGCATCCCCTGGGAGTGTGTTAGAAATGCAGAATCCCAGGCCTCACCCCAGACCAGCCG
AATCAGAATCAGCGTGGTAACAAGATCCTCAGCAGATGCGTAGACATGTGAAAATTTGGGGTGCGCTAGT
ACAGAGATGGCCACCAGATACTTGGGAAAGTCTGGTCAATTACTTTTCCGTTTCACTTGTCATCATCTAA
CCCATTTTGTGGGTATTCGTTTCCTGTCTGCCGCTACCCCCATGTTCTACAAGCGCAGGCTTTGATCTAT
TTTCCTCACTGCTATGTAGTCAGTAGGTGCTCAGTGAAAATTAGTTGAATGAATAAATACATTTTTAAAA
CTCTCAGGCTGGGCATGGTGGCTCCAGCCTATAATGCCAGCACTTTGGGAGGCCGAGGCAGGATCACCCG
AGGCCAACATGACGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCATGCGTGGTGGCACACATCTG
TAATCCCAACTACTCGGGAGGCTGGGGCATGAGAATTGCTTGAACCTGGGAGGCAGAGGTTGCAGTGAAT
CGAGATTGCACCACTGCACTCCAGCCTGGGAGACAGAGCAAGACGCTGTCTCAAGAAAAAGTAATAACAA
TAAACCCTCCATGTGTCCTCCAGGATCATTTCTCTGCTGAGCAAGTTCCGACTGGGATTCCATGAAGTCC
ACATCCTCCCTGACATCAACCAGAACCCTCGGGCTGAGCAGTAAGTTCTGTTTTGGGGCTTCCAGGCAGA
AGGGCCAACTGTGTGCCTGGAGACCCCTCTGCCGGCTGCCCCCTTTTTCCTTGGTAGACTAGGAGCAAGG
GTCTCTCCTCCAAGCCCTTCCTAGATGTGTCAGAGGGCACAACTTCTGCAGTGGCTGTGGCCGGGGCCTG
TCCCCTCCCTGGGGCTGAGGGGACCACAGGGCAAGGCGGCTGCAGCCCCTGACATTGTGTTAACACATTG
GTGCTGGGAGAGGGGAAAAGGTGAGTGACCTGGGCAACAACTTCCTTCCTCCTTCCACGCAAGGGCAAGA
GCACAGATTTTAAGCAAACCCCTGTGTGAATTTGTAGCAGTGACCAATGGCAGGTTCAGCAGGGACTGTT
TGCTTTCCAAATGGTATAACTCCGTCTTCTTCAAATAAGACATTTACAAACAGGATTTGTATTTTGTCAA
TCAGTGTGCGTGAGGTTTTCCAGGACTGTCTCCTCACTAAGGTATGTTTCCTCCTGACCTCCTAGCCCTA
ACTTTTCTCTTACGTCTCCCAGCCGTAAGGATGTCCCCCATCTGGACAGGGAAGGAAATCAAGACAAGCA
GAAAGGAGCTGTGTAATAGTGATGCATACATTGGCTCCAACCATTTGTTAGGAGCTGTGCTTGGTGCTGC
AGAGGCAGAGATGAGGAATCTGCAGCCCCATCCTCCAAGCATGGCTGCAGGCTGGTGAAAGCCAAGGGCA
GTTGCAATGCTGCAGAGACAGATCAGGGTGCCAAGGAGAGTAGGATTGTCTCTGTTGGTGGAAAAGCAAA
GCCTTGCGAAAGGCTGGCCTTGATGATCAAAAAGAGATCAGGGAGATCTTCCAGGAAGAAGAGACAGCAG
GAGCAACATACAGACCCCATCTAGCATCTGATTTTTATTTATTTATTTATTTATTTATTTATGAGATAGA
GTCTTGCTCTGTCACCCAGGCTGGAGTGCAGTGGCGCAATCCCAGCTCACTGCAACCTCTGCCTCCCAGG
TTCAAGCAATTCTTCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGAGCACACCACTGTGCCCGGCTA
AATTTGGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAG
GTGACCCACCCGCCTTGGCCTCCCAAAGTGCTGAGATTACAGGCGTGAGCCACTGCGCCCAGCCTAGCAT
CTTATTTTAAAAATCCCCTCCATTGACCTTTCATCTGCCTCCAGCAACTGGACCATTTCTCTCCTCCTTG
GCACAGCCAGACTTCAAGATGTTTGCACTCTTTGTCGACCCCTCCTCCCTTCCCCTAATCCTCCCGCTCC
CAACCCACTGCCCCCGCTGTCTGAGACCACTCTTGCCAGCATCATCAGTGACCTCCACGTTGCCACACCC
ACAGAGTCCTCTCTCTCTCTACCTTTCAGCTGCACTTGACAGTGATCCTTCTTGAGACCGTTCCTTCTTT
TGGCTTCCAAGACACCCCTTCCACTGGTTTCCTCCTGCATCAGGGGCCCTTCCTCCAGTTCGTCTGCTGG
TTCTTCATCCTTGACCTCACTCGCCCTTGCTGGCATCCTTGGGGTTTGGCCCTGACCCTTTTCCCTTCTC
TCCATTTACTCTGTCCCAGGTGAGCTCATCCATCCTTGTGGCTTCAGATAGCATCATCTGAATGCCAATC
AGCCCTACATCTCTCTCTCTCTTTTTTTTTTTTGAGACAGTCTCACTCCATCACCCAGGTTGGAGTACAG
TGGTACCATCTCAGCTCACTGCAACTTCCACATCCCAGGTTCAAGTGATTCTCCTGCCTCAGTCCTCTGA
GTAGCTGGGATTACAGGTGTGTGCCACCACACCCAGCTAATTTTTGTATTTTTAGTAGAGACAGCATTTC
GTCAGGTTGGCCAGGCTGGTCTCGAACTTCTGACCTCAGGTGATCCGCCCGCCTCGGCCTCCCAAAGTGC
TGGGATTACAGGCGTGAGCCACTGTGCCCGGCCCCAGCCCTAAATCTCTGTCTTTAATGATGTGAGTCAC
ATCGCACAGAAGACCCCCAGCAGCTTCTGGTCTCACTTGGGGCAGATAAGGAAGCCCTGCCCGACTGGGC
CCTCTCTGCCTGCCTGTCCTCACCTCCTCCCACCCTTCCCTGCACTCACCTTGCTCCAGCACACAGGATC
CTTCACTGCTCCTCATATGAGCCAAACACGCTCTGCCTCAGGGCTTCTGCACGTTCAGTTCTGTTCGCTT
CTGCACCCCCTGTCCCGTGCATGGCTGGTTTTGCAAGGACAGACTTCCTTCAGGTCCCAGAAGGGCCTTC
CCCACCCATTATTCTCTGTCCCAGTACTTGGTTGTCCCTTCATAAGCTTCCACTTCATATTTATTTGCTA
CTTGCTTATCACCGTGGCTCTGTGAGGACTGGGGACACAATCTGATTTGTTCACTGTTGTGTAGCACAGA
GCCTGGTACAGAATCATGCTCTGTAAATACCTGCTGGATGGTCGGGTGGGTCACAGGTCAGTGGTTGTGG
GCAACTTGTGAAAGTTCAGTTGGATGCCAGACCCAGCCAGCAGAGCTGAATTCAACATGGAAGCCACTTT
GTAAATGGCAGTGTCCGATGGGTTTCAGCCTGAAAATGGGAGCTGTGGCAACACTGCCAGCTCCCCGCAG
GGACCTGGCACCCCTAGAAATGGGGTTATCGTCTCAGCCGGCCTCAACCCACTTTCTCGTCCCCAGCACC
AAGAGGTTTGAGGACATGATTGCACCCTTCCGTCTGAATGATGGCTTCAAGGATGAGGCCACTGTCAACG
AGATGCGGCGGGACTGCCCCTGGAAGATCTCAGATGAGGAGATTACGAAGAACAGAGTCAAGGTGCAGAG
AGGGGTGGGGGTGGGAAACGCGACACATCACTGGGTCAGGGACGGGTGTCCTGCATGTCTTGAGCTCCCC
CAGCCCCTCCCCTCGATCCTCCACCCTGCCTTCCACTCCGGCCCCTGAGGTATCCTCAAGCCACAGTCGT
TCAGGCTGATGGGTAACCCGGCTGTGGAACTCAGTGCTATCCTTGACCTGGGAGCCTGGTCCTCCTGCCC
ATCTTGAGTGAGCTCTGGCAGTGGGCTTGACTGCCCGGAGTCCTCCTCCTCCTCCTCCTCCTCCACACTT
CCTCTATGCCGGGTGGAAGAGATTTCTGAGACTCATGGTGACAGCCAAGGTATAATTAGTCCTGTTAGGC
CGGGCATGGTGGCTCACCCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGTGGATCACTTGAGGTCA
GGAGTTCAAGACCAGCCTGACCAACATGGTGAGACCCCATCTCTACTAAAAATACAAAAAAAATTAGCTG
GGCATGGTGGCAGGCACCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGG
AGGCAGAGGTTGCAGTGAGCCAAGATTGTGCCATTGCACTCCAGCCTGGGTGACAGAGTGAGACTCCGTC
TCAAAAAAAAAAAAAAAAATTGTCCTGTGGGCCAACACGGCTGCCTGACCTTCCCTGCTTAGCCTCTAGA
CCCTACCCCTGCCACAGCTCAGAGCTATGGTGCCCCTTCCTGGACAGCACCTTCAGGACTCTAAGGGCTA
AGAAAGGGTGTGTGCCTGTAGCCATGCTGTGAGTGCGTCAAGGTGGGGCAGGCTCTAGGAGTGCGAGCGA
GAGCGATGGGGGCAAGGGCCGGGTCCTACTTCTACCCCCAGTGTCAGTCATTCTCCACCACGAGGGAGCT
TACCCCCTGGGGGACATTTGGCAACCTCTGGAGACATTTTTGTTTGTCACAACTCGGGGGTGGGGCTGCT
ACTGTCAGGCAGTGAGGAGAGACCTGCGATGCTGCCAAACATCCTACGATGTGCAGGACACTTCCCACAA
CAAGGACTTGTATGCCCCAAATAACTACAGTGCAAGGTCCAGAATCCCTTACCCACGTTAACTCCCTTCC
CCTCGGTCTCTTGGTCTCCAGGATAGGGCATCCGTCCACGATCAGTTCCTGAGTGTCAATCATTTCTGAA
TAATTTGAGCTAGGCCTTGGGTTGGAAGGAGTCTCCCACCCCCAACCCAGTCCCACGCAAGTTTGGGGAC
TTCCCTAATCTGTCAGCCCCGAGACCCTGCCCCAAAGGGCCCATGCAGCCATCTGGCTCTTTGGAAAATG
CTGAATAAAACATTGTGTCCGAAGAAGACCTTCCAATACTCTGTTACAGAAGAAGTGGGGCAACCTCTCT
TTTATAAAGTATTTTTAGTGTACCAGGCCCTCTGCCGGGTGCTTTTGCATAGGTTGTCTCATTTGATCCT
CCCCAAATTCCCCTGGAGAAGGTATCATCATACCCATTTTACAGATGAAGATGGTGAAGGTCAGGGAGAC
AACCTGACTTGCCCAAGTTCTCTCTGCTATGAAATGTGAGAGCTGAGATCTGAACCTAGTAAGGTGTTCT
CTCTTCCCTCTACCCTGCTGGTTATGGTCATAAAGTCAGGCAAGGAAAAGCCACAGAATGCTTCTTGGAG
GAGGTGAGCTTGGTGCTCCATTACTCTGAGGGTCCCCTTCTTCCTGGAGACAGGAGACTCTATAAGAATT
TATGAGGCAGCAGAGTCTACAAGTAAATCATGAATCCAGTTGAAAATGTTAATGAGGCCATAGACGTGGT
GAAGGATTGAGTGACCTCGATGATATGGGAAGTGACCACTCGGCTTTCTCCCGCCCAGTCCCTTCGGCAG
GTGAGGCTGAATGAGATTGTGCTGGATTACTCCCGAGACGCTGCTCTCATCGTCATGTAAGTAGTGCCCG
GCTGGTGGGAGGACCAGTCTGTCCAGAGTCAGGTGTCTCAGCTCTGGGAAGGGGCTCAGCAGGGGCACCA
CGGAGGGCCCAAGCCTTCCCCTAGGAAGCAGAAGGGCCAAAGTTCCCATAAACATAGCCCTGGCGATTCT
TAGCATGTGGCTGGGCCTGAGCCCGCCCCACACCTGAGCTCCAGCTGGCAGGGGTCGAGGGCCTCACGTG
TCCCAGGCATCAGTGACCTCCCCTTGCCATTGCTTTTTCCTGTTGGCTGCCTTTTCCTACCAAATTCCAC
TTGGCAAAGAGGGAACCCAGCTCTTTCAAGAGGGACATCAACTCCTGGTTGTTCAGGAATTACTTGGCCC
TCCTTGGCTATCGGCCATTCCCAGCTGATGTTGGTTCCAAGCCTGAGCAACAGAAACAGAGGCCTGTCCT
AGCCCTGGCTGTTCCTACTGGACCCACGTCCAGTTCTGAGCCACCCCTGGGTGTATTAGCCAGGGCTGAG
CAAGCAGAATGTGGGATGGAAGGTGAGAGCTATGCAGCAAAGGTCTCAGGGTGGCTCTGCTCTCTCTGAG
GCCTGTAAGAAGAACATGATTGGCCGGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTG
AGGCGGGCAGATCACCTGAGGTCGGGAGGTCGAGACCAGCCTGACCAACATGGAGAAACCCCGTCTCTAC
TAAAAATACAAAATTAGCTGGGCGTGGTGGTGCATACCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAG
GAGAATTGCTTGAACCTGGGAGGCAGAGGTTGCGGTGAGCCGAGGTTGCCCCATTTCACTCCAGCCTGGC
AACAAGAGCGAAACTCCGTCTCAAAAAAAAAAAAAAAAAAAAAAAGAAGGAAGAAGAGGAAGAACATGAT
CAGCCAAGCCCTCAGCTCAGGGGACAAGGGGTCTTCGCCAAGGTAAGTAACTGGCCTCTCCTACATTTCC
TGGGGGCCAACCACCTGGGAATCAGACAGAAGGGATCTTTCGGCTCTGGTGATGTACCCCACTCCCAGAC
CTGTCACCATCTGACCTGGACTGCAGATGCCAGGTTCTGCATGACAGCCGAAATGTTCGTGGAAACTGCT
TGTTCAGAGCCTGAAGCCAGGACCAACTACACAATTTGCAAGGCCTAGGACAAAATGAAAATGTGGGGCA
CCTTGCTCAGAAACTATTAAGAAGGTCTGTGGTCTTCCCTTTCAAAGCAGTCTCCCTTACATCCTCACAT
AGGATGTATGGAATTTGTTTTTTAAAAAATCTCACAGTGGTGGAAAGGAGGAGGGGGTAGGGGTTCAACA
CAACTGATCATAGTTTGATCATTGATGTAGACAGATGATGACGTGGGCATGAAACGTCAGGGTATCATTT
TTCAAACTTCACCTGTTTGAACCCTTCTATAAATCTGCAAAATGCATCCTGCCTTGGAGAGCAACCACAA
GTGCCTCATCAAGCTTGCTTTCTCTGGCAAGAAGGTCCCCAAAAACCTAGCGCCACACACTGTTCTAGGA
AGATGTGCTCAGATAGGAGGGATCCTTCAGCTCTGGTGATACAACCCATTCCCAGACTTGTCACCATCTG
ACCTAGACTGCAGATGCCAGGATCTAAGGATATTAGATTCATGAGAGGAGACCTCAGAACAAGTGGTCCA
TTGAGGGTGATAAAAGCAACTCAAACTATTTTCCCCCTACTTCTGTTGCTTGAGTACTGTGTACGTTGCA
TGCATCCCTTACTGCTGTGTCTGCAATACCCTCTTTCAGGAGAGCACCAGCAATGTTTCTTTTATGTAGT
GTAAGTGCCCCTTTGAGAAGATCAAAACAGCAAATGGGTTCAGACTGAAAATGGCCAATGGCTTCTAGCA
GATGAGTAGATAAAGCTTGAGGATACGCATCCTCCTAGGTCTCATCAAAGGCTAGAAGCTGAGCAACCAG
TTTGGAGACCTGACCTGGAGGTTTCTGGACCTCCAGTCTGTCTTAAGATGTCCATGAGGGTTTTTCCTCC
CTGGATGTCTGAGGATTGACTGCCATCCTGAATGAATCAGTAAGTATCCAAACTAGGAAGGTCGTGACCA
ACTGACTACTTCAGAGGAAACAAATGGTCGTCTATGTCCTTCACCCTGGGAAGGCAACAGTACCTAAGAT
AGAAATTCAGGACCAACTAGCCAAAATATACAAGACCACACTGGATATCATCTTTGCATTTGGATTTAGA
ACTAATTTTGGTGGTGGCAAGACAACTAGCTTTGGCATGATTTATGATTCCTTGGATTATGCAAAGAAAA
GTGAACCCAAACATAGACTTGCAAGACATGGCCTGTATGAGAAGAAAAAGACCTCAAGAAAGCAATGAAA
GGAATGCAAGAACAGAATGAAGGAAGTTAAGGGGACTGTAAAGGCCACGTTGGTGCTGGCAAAAGGTGGG
CTGGAGATTGGATCACAGCCAAAGGAGTAAAGGGGCGGCAGTGTGTTATCTGCAGCCATTGTGGATTTTT
TACAAGAAGATTAATAAACTAAAAACTCTCACATGAAAAAAAGATGCCCAGGAGAATTCAAAAGGCACCA
GCACCAGTTACTCTTCATTGACAGGACAGAGGTAAAATTTTTGCTTTTACCAATTAGCGGAATTTTGTCA
CCAGGAGAGTGGTTCAGGCAATTAGAAACGTGCTTGGGGCTGGGCATGGTGGCTCACGCCTGTAATCCCA
GCACTTTGGGAGGCCAAGGCGGGTGGATCACCTGAGGTCAGGAGTTTGAGACCGGCCTGGCCAACATGGT
GAAACCCCACCTCTACTAAAAATACAAAAAAATTAGCCAGGCTTGGTGGTGCACACCTGTAATCCCAGCT
ACTTGGGAGGCTGAGACAGGAGAATTGCTTGAACCTGGGAGGCAGAGGTTGCCGTGAGCCAAGATCATGC
CACTGCACTCCAGCCTGGATGACAGAGCTAGAAATGTGCTTGGATAATACTCAAACGCTACTTCCATACA
CGACAGCAGTTTTCAGAAATCATGTCTTAGAACATTCAGGCTGCTATAGCAAAATACCATAGACTGGGCT
TATCAATAACAGAAATTTATTTCTCATGGTTCTAGAGGCTGGGAAGTCCTAGATCAAAGCATGGTAAATT
CAGAGTCAGAGGCAGGGCTCCTTTCCTGACTCATGGATGGGCCTTCTTTCTGTGTCCTCACTTGGCGGAG
GGAGCCAGGGAGCTCTCTGGGGCCTCTTTATAAGAGCACTAATCCTATTCATAGGTCACAGGGCTCCACT
CTCATAACCTCATCATCTCCCAAAGGCCCCACCTCCTAAAACCATCACTCTGGGGATATGGATTTCAACG
TGTGAATTTTGGGGGGACATGAATATTCAGACTGTAGCAAATGGTTCTGACTTATAGTTTCTCTTCTTCC
CAATCACTTCCTATGTGCCAGTAGACAGTAGAGCATGTAGAATTCTTCATGATTCCTAATATCCTATGCA
GTGTGCAAACCCTTCTTAGCAGATGTGTGTAAGAGTGCAGAAGTCTAACAACAGACCAGAAGCAGACCAT
TGTCAGTCTGCCCCTGGGACTCAGTATTCCTCATGCCCCGATGTCCCCAGTGCCAGTGTGTGCTTTTTTT
TTTTTTAAAGAATGATTGTATTCATTTCTCAGATAGTGATATAACTTTTTTTTTTTTTTTTTTTGAGGCA
GAGTCACTCTGTCACCCAGGCTGGAGTGCAGTGGTGAGATCTCAGCTCACTGCAACCTCCGCCTCCTGGG
TTCAAGTGATTCTCCTGCCTCAGCCTCCTGAATATCACCACCTCGCCCGGCTAATTTTTTGTATTTTTAG
TAGAGATGGGGTTGCACCATGGTGGCCAGGCTGATCTCAAACTCCTGACCTCAGGTGATCCACCCACCTC
AGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACGGTGCCCAGCTGCAGATAGTGATATAATTTATA
GTGTAGTCAATTGTGTTGAACTGCCGCTTGCAACATTTTCTTGTGTGACTTAAGGCATTAGAAAAAAAGA
AAAAAAGAAGAATGTCAAGACGGTGAGAGCAGGGCATTAGATAAACAAGGGACCCTCCGGAGCAGAAGGC
CCCGTGTGACTGTGCTGGTTGCACACCCAGGAAGCTGGCTCTGCCCAGAACAATGCTTCCTGGGGCTTTA
GGGACATGATTTTTTTGTACAAAGAAAGTGCCCCGTGTTTCCTTGGAGAACAGTGATAAGGAAAGGGGCT
GGGCTTATCTCCAAACCTCTTGCCAACACCTAGCAGAGAATGTTCCTAGGACAAGATTGGTCCAGGGTGA
GCGCTGGGTCCGAGCGCAAGACAGTGGAAATGGCTTTGTCCTGGGACGCCTCCCCAGACTCTTCCTTGGG
TATGGTGCCTGGTGGGTGTGCATGGGTGTGCCAGACCACGGCTTAATGAGTGCCGCCTCTGTTCAGAGAG
GCATGTGTGCATTTTGTCTATGCGATTAGCCCACTGTGTGGCCTTGGGTAAGACCCCATGTCTCTCTAGC
CTCAGTTTCCCTAATTATAAAGAGTTAACAAAAGTATTTCCAAGATTCTCTTTCATGCTAACATGCTGTG
ATTCTAAGTTTATTTGTCATTAAAATGAGTCTCAGCTGGGCATTTATTTGTCATTAAAATGACTCATTAA
AATGAGTCTCAGCTGGGCATAGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCTGAAGGA
TTGATCGAGCCCAGGAGTTGGAGAACAGCCTGGGCAACATAGTGAGACCCTGTCACTACCAAAAGAAAAA
AAAAGAAAGCAAAAAAAAAAAAAGAATCAGTCTCTCAGTCTCTTAAGCCATAAGATGAAGAATTTCATCC
CGGGAGGTGACCACCTGTTGGAATCTCTCCCTTCCCACCCCCCATCCCTACCACCATCCCCCAAACTCAC
AGGGAAAATCAATCCCCCACCCACCAGCCTTTGTGGGATTTGCCAATACAAGTAATTATAAATCCAGTGA
CCCAAGAGAAGACCCCTGCCCAGGGCGTCCCACCCCATGCCAGTAGCTACAGCTGTTGCCCAAAGAGGCC
CCAGCTATTGGGCCAAGTCCACCGGGAGGACAGAGCAGCTGCATCTCTCCATCACTAATCTCCAACCTGG
AGCCATGCCTAGTGTTTCTCTACAAGTTCAGTCAGAGGGTCCGCTTCATCAGAACCACCAGGGGACTGTG
AAAAATGCAAGTTCCTCAGTGCCTTCCAGACCAGGCTCTAGGGTGGAGCCCAGAAATTCTGAGGAAGGGA
AAAAAAAAAAATCTCCCAAGCCTCGGTGAAATGATTAGATCACATTGTCTAGCATCTGGGAGAGCGTGCT
TGGAGCATTTTCTAGAGCCGGTTTGAGGCAGCACTTGATTGATTGCTGTTCACTAATCAGTGGGTTATCA
AATGGCCCCGGGTTTGAGCTCAGCTACCCCAGCACAGCTGATAAACCAGCCTCATAAAAAGCCCAGCAAG
CTTTGACTAGCCTAGCAACATATTCCTGTTGGGTCCAGACACCTCTTTTTTTTCTAAAAACACTCCCTCC
TCAAAGCCAAGCAGCAATAATCAAAATAGTAATAATCAACATTGCATTTTATGGCTTGGGCCTCTATATG
GTACTCAAGGCAATAGGCCTTATGAATTTGCAAAGCCCTAATTTTAAGAGAGCATCTCACTCTGTCACCC
AGGCTGGAGTGCAGTGGCAGGATCTCGGCTCACGGCAACCTCTTTGTTTTTTTTTGTTGTTGTTGTTTTT
TCTCTTTCTGTAACCTGTGACTCAGGCCTCTTTGGGCATCTTTGGTCCTATAATTTTCTTTGTTTTGGTG
GCGGGGTCTTACTCTGTCACCCAGGCTAGAGAGCAGTAGTGAGATCTCAGCTCACTGCAACCCCCACCTC
CCGGGACTCAAACAATCCTCCTACCTCAGCCTCCCGAGTAGCTGGGACTACAGGCACATACCACCAGGCC
TGGCTAGTTTTTTGTATTTTTTTATGGAGACAGGGTTTTACCATGTTGGCCCAGGCTGGTCTCCAACTCC
TGAGCTCAAGCAATCCACCTGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGTGCCTG
GCCTCAAAACTCTATTATAATCACCCCCTCACTCCCACTTTAGAGATGAGGAAGCAGCTTCAGAGAAGTT
AAGCAACTTGGCCAGGGTCTTGCAGACAGGAAGTGGGAATTGCATCTGGAGCCTGTGACTGTGGTTCTGA
TGCAGGGCTGTCTTTCACATCTAGGTTAGATCTGAAGTCGCCTTTCTTTGTGGCTAAGAGATGTAATTCT
GAAAAAATGTTCCTTTGTTCTGGAACAGTTTTATTTCTGAAGATTAGTAGCTCAAGGCATGCACATCCTG
TGAGCAGGAATAGAGCACGTCATTGACTGGTGTGGGGGCGGGGGTGATTTTCAAGACAGCTGTCTCTCGG
ACAGGTGGACAGGCTGTGTTGGGGGAGGGAACTCCATGCTGAAGCTTGAAAGTCTGACATAGGTGTGTTG
GATGTTCCCCACCCCCATGGTGCAGGCACCATTTCTCTCGTGGCCTTTGAAGTTTCTGAATTCAGTCCCC
GAAATCATGTCCTATTTCCTGGATGTTGTCCGCCTCTTGCATAACTGCTCAGTCAGGAGAGTTTGGGCTC
AGTACTCCTATGTCCCTTTCTCCTGGAAAGTCACTAAATATTTGAACTCAGATGACTATAGCTTAATGAA
ATGGCCTTGGTCCAACCCACCTTCCCTAATTTTTGAGACCTTTTGTTTGTTTGTTTGTTTGTTTGTTTGT
TTGTTTTGAGACAGAGTCTCACTCTGTTGCCCAGGCTGCAGTGCAGTGGCGCAATCATGCTCACTGCAGC
CTCGACCTCCCAGGCTCAAACGATCCTCCCGCCTCAGCATCCTGAGGAGCTGGGACCACAGGCGTGTGCC
ATCATGCCCAGCTAATTTTTCTATTTTTTGTAAAGACAGGGTCTCACCATGTTGCCCAGGCTGGTCTCAA
ACTCCTGGGCTCAAGTGATCTGCCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGACCATGCCT
GGCCCGGGACATTGCTTTTTAAAGGAGGGTAGGGCTTGTCCCAGGTGAAGCTTTGTGGATGGAACTTCCA
AGTGTGACATAGCTGTTTAGTATCCCAGTTACCCTTCTCAGAGGAGGTTGGAGTTGCTGTGAGCAGAGGC
TGCTGCGGAGGGAAGATGTCAAGACCTCGGTTCGAATTCCAGCTTTGCCGCCGCTGGCTTAGCTGTCGCG
TGCTGGTCAGTACTAAGTGGTGATGACAGCTGCTGACCTTACAGAGCACTCACTGTGTGCCAGGCATTGT
GCTGTGCCTGCGTGTGCTTTGCCTGTTTTAGCATCACAAACATCCTGCGAGGGAGGTTTTTGTGTTCTTG
CTTTAGGGAAGAGAAACTCTGCAAAGGTCATCTGGCTGAGGAAGTGGCAAAGCCACGACTCGGACGCCAG
AGACTGGCTCTGACCTCAGCGCTCCAGCATCCTCCTCCCGCCCCAGTTCTCCCACCTGTGAAGGAGCGCG
GTGCTGCGGGCCCTGCCCATTCCCCAGGGGGATGTACTTGGGGAAAACAGACACTCAAGTGAGTCCCACG
GAAGGTGCTGGTGCCCCCACTTCATGCGGCACCAGCTGGACCTGTGAGACACTGCTGGAGCCCACAGACC
CTCCTGGAGCCCAGAGCTGGGCAGCTGTGGCCATCCCAATGCATTTTTGGAATTCTCCAGATAGTAGAAA
AGTCACTTCCTGAAATAGTTGGAAGACATGGCTGGACAGCATTCCCAGGGCATCTTTTTATTCACGCAGC
ATTGCACAGTTAAGACAAGGGGCCTCCCCATAGGCAGCGGTGGAGTCTTGCTCCCAGGGCCTCCTGCACA
GGGCAGGAGGCAGGACAAAGACGCTGAGCTGGACAATGAATGAACAGGAACATATCACATTGCTGTGGCC
ACAGGCTGGCACAGGGCTGGAGAGGCAAGCCTGGCATGAAGCGGGTGGCCTGATAGGGAAGGGTCGCCCA
CTTAACAAATATATACTGAGCCTGACTGTATCAGACTGTGTTCTAGGCACTGGGAAAAGGGGGGTGAATA
AGGCAGATCTGGCCTCCTTGTGAGCGAACATTCTAATGTGGGGAGACAAGCACATAAATCAGAAGATGCT
GTTGGGACTGTGGAGGGCTCTGAAGGCAGTAAAGTGGGGTGATGTGAGGAAGAGGCGTGGGCGGCCTGCC
CTGGAGGAGTGATCAGGGAGGAGGTGGGTGCACTTGCCTGACTCAAAGGAAGTGACCAGGTGCAGAGCTG
GGGCTGCTCCTTTCCTGGTGGGTGGGGAGAACGTCAAGTGCAAAGGTTGGAGGCAGGAGTGAGCTTGGCA
TTTTTTTAGGAATGGAAAGAAAGCCACAGGGCATCGGGGGTGAGCAAGGCGAGGGTGGGAGGAGGTGAGG
TCGTGGGCTTGGCAAGGACTAGATCCCATAGGGCTTGTGGGCCATGGCTTTGTTTAGAAACGCTTCACGG
CAGAAGGTCCATTCTGGGTCAGGTTTGTGCGGAAAGTGGGGAGGTCATTCTTAGGGTGGGTGGAAGGAGC
TCTGAGGGACGGTAAACAGACTCGCTGTTCTGAAAACAAACTGGGAGCTGGGCGTGTGGAGCGGCCCCGT
GGTAATCTCTCTTCTACCACTTTTTCATGCCTTGCAGCACTTTGCCCATAGGGAGGAAGGGGAAGTGCCC
CAGCTCGCTGTACATGGCCTGGCTGGAGACCCTGTCCCAGGACCTCAGACCTCCAGTCATCCTGATCCGA
GGAAACCAGGAAAACGTGCTCACCTTTTACTGCCAGTAACTCCAGGCTTTGACATCCCTGTCCACAGCTC
TGAGTGTGTGGGATAAGTTGGAACTTGATTGCCTCTAGTCCACAGGGATGAGACTCATGTTCTGTTGCAC
TTTAAGTGGCAGCATCTGATGATCTCACCGAAAAAGATGGTAGATTTCCAAATCTGGCTGGACTCCACTT
CCATGGGACACATTCCCTGGGTCTTGTGTTTATAGGCTAGAGAAATAGCAGATGGAGCTGCAAGGAAAAC
TCTCTAAAGCATCCTATTCCTTTTAAAGGATTTCTTTTGATTTTGATGACCATTAATTAAGAGTTCAGTC
TTTGATTTGTATGCAAATTGGAGTCCCAATGCTGGGCGTGAATCTTGACAGTTTCTACAGACCTTCCTGG
GTGAAAGTTCCTAAATCATGCCCTGCTTCCTCCAATAGGAGAATGGGAGCCTCACCTGTAGGACCTACAG
GCTCTCTAAGGAATGCAGGTCTCTCTCTGAGCCTCCACAGCCAGGCAAATACATATATATATATTTTTTT
TTTAGATGAAGTTTTTTCTCTTGTTGCCCAGGCTAGGGTGTAATGGCATGATCTCAGGTCACTGCAACCT
CCTCCCGGGTTCAAGCATTTCTTCTGTCTCAGCCTCCCGAATAGCTGGGATTACAGGCACCTGCCATCAC
ACGAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACCATGTTGACCAGGCTGGTGTTGAGCTCCT
GACCTCAGGTGATCCACCCACCTCGGTCTCCCAAAGTGCTGGGGTTACAGGCCTGAGCCACTGCGCCCGG
CCCAGGCAAATTTCTTGAACCACTTCTCACTCCCGTCACTTTCAATAAGGGGTCTTTGATGTCTTCACTG
GTTCTTTGGACGAGGGACTTTTCGAACTTTTTTGGTTGCAACACACAGTAAGAAATATACTTCACACTGA
GACTTGCAGCGCACACACACGGAAACGACCAAAACAAAAATGTCACAAAACAATACTTACCCTTCCCTGG
GGGACGTCCTCCAGTATGTTCTGTTCTGTTTATTTTTCACTGTTGGTTGCAATCCAATAAAATGACTTTG
GGATCCACTCATGGGTGGGGACCCACACATTTGAAAGGCATGGCCACCTTTCTGTTGTGCCTTGCATTTG
TCCACACACAGGGAGTCTGGCTGAGCTGGGGAAAGGCCACGGCTGGGTGTCATTGCCATTTTCCCAGCTC
ATCTCACCGGGAAGAAAAGCAGATTGACAGAACACGTGAGGAGGGGTATTGATGGCAGGAGAGTCAAAAA
AGAGTTTTAAAGAAGGGGCAAGGTTGAAGGAGTCTAGTGGCAAGGGTAAGATTTCAGGCATGGTTAAGAA
CAGACGACAAGGATGTCAGGAATGAAGATGTGGAGAGGGGTGTAGAGATGGCAAGGTTGGCAAGGAACAG
ATAGGCAGGAGCAGGTCCAAGCCAAGCCTAGCCCAAGACCAGGTGAAAGGAGAGGGGAGGAGGAGCCACC
TGCAAGAGATGGAAAGAGCAGGCGGCAGAGGGGGCTGGCAGGGAGGGGCTGTTAAGAGTGGGGTTGGAGG
TGGGAGAGAAGCTAGGACAAGGGAGATGGAGAAAGGACCTATACCTGGCTCACGGAAGGCCTTCAGGTCA
CTACACGTTGAACATCCCCAGTGTTTGAGCCCCCAAAGCTAGGGTGCAAGAGCACTGCCATCGAATGCCA
GTGGGTGAGGCCAAGTGAGGGTATTTGCAGCTCTAGACATAACCAAGAAGCGTAAAGGTGAGTTGTTTGG
TGGTACGACTGCCTGTGCCTTCTTCCGATGGCACTGGGGTGGCTGAAGGAACAGACATCTTTGGGTTTCA
TCAGCCTCCTCCAAGACTGCTGCAGTGCCTACACTTTAGACTTCAGAAGGAGACTAAAGACTTCTAGAAT
TTAGAAGGAGATCTGAAGTCTCCTTTCTGGAGTTACAACCCAAAGGATGTTAGCATTTCTCAGGTCATCC
CACTGCAAAGCCCAGAAGGCTTGGGGCTCCCAGGCTGCTCTGAAGCCCCACTGTCTGACCGCCTCAGGGC
TTGCTACGAGGGACTGGGGCACGGCCAAGCTGACTAGGAACAGCTCTCGTGCTCCTGAGGGACCTGGAGG
ATGGGCCTGCCTCCCAGCCATTGAGCTGGATTCTGGGATAATTCTTAACTCGAAATAAGGGGAAGCATCC
ATCAGGGAATGCTGGCCTTTCTAGAGCCACGTAGAAAACAATTTTCTGGTTCTTCAAACCTCAAAGAGTC
CTTGGTCCAAAAAACAGAATGTTTTGGCTTCGGGTGTCAAAAAAAAAATTTTCACGATGTCAGAAATAGT
ATGTTTTTAACAATAGTAATAGCTTTGTAAAAAAATAAAAAGCTTTAACAGCGAGGCCATAAACAATGAA
ATGAATAAAAACGGTGGTCATTCAGTCAACGGAA

Solute carrier family 12 member 3 isoform 1 [Homo sapiens] Secvența aminoacidică

>NP_000330.3 solute carrier family 12 member 3 isoform 1 [Homo sapiens]

MAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSHPSHLTHSSTFCMRTFGYNTIDVVPTYEHY
ANSTQPGEPRKVRPTLADLHSFLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVK
GVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRS
LGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPINDIRIIAVVSVTVLLAISLAGME
WESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFFGMFSIFFPS
ATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLA
CSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYP
LIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRP
SFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVG
LNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWL
NKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYG
VCVMRMREGLNVSKMMQAHINPVFDPAEDGKEASARGARPSVSGALDPKALVKEEQATTIFQSEQGKKTI
DIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQ
NPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAA
LIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Protein Structure
Transcript table

Name of the Transcript length,

Protein length, aa UniProt ID
transcript bp

SLC12A3-202 5567 1030aa P55017

SLC12A3-203 4208 1021aa P55017

SLC12A3-205 3119 1029aa P55017

SLC12A3-201 4208 1020aa J3QSS1

Tabel. Procese biologice în care este implicata SLC12A3

Accession ID Term Definition of the biological

process
GO: 0006811 ion transport The directed movement of
charged atoms or small
charged molecules into, out of
or within a cell, or between
cells, by means of some agent
such as a transporter or pore.
GO:0006814 sodium ion transport The directed movement of
sodium ions (Na+) into, out of
or within a cell, or between
cells, by means of some agent
such as a transporter or pore.
GO:0006884 cell volume homeostasis Any process involved in
maintaining the steady state
of a cell's volume. The cell's
volume refers to the three-
dimensional space occupied
by a cell.
GO:0055064 chloride ion homeostasis
Any process involved in the
maintenance of an internal
steady state of chloride ions
within an organism or cell.
GO:0055075 potassium ion homeostasis Any process involved in the
maintenance of an internal
steady state of potassium ions
within an organism or cell.
GO:0055078 Any process involved in the
sodium ion homeostasis maintenance of an internal
steady state of sodium ions
within an organism or cell.
GO:1990573 The directed movement of
potassium ion import across potassium ions from outside
plasma membrane of a cell, across the plasma
membrane and into the
cytosol.

Table. Funcții moleculare ale SLC12A3

Accession ID Term Definition of the molecular process
GO:0005215 transporter activity Enables the directed movement of
substances (such as macromolecules,
small molecules, ions) into, out of or
within a cell, or between cells.
GO:0005515 protein binding Interacting selectively and non-
covalently with any protein or protein
complex (a complex of two or more
proteins that may include other
nonprotein molecules).
GO:0008511 sodium:potassium:chlo Enables the transfer of a solute or solutes
ride symporter activity from one side of a membrane to the other
according to the reaction: Na+(out) + K+
(out) + Cl-(out) = Na+(in) + K+(in) + Cl-
(in).
GO:0015081 sodium ion Enables the transfer of sodium ions
transmembrane transporter (Na+) from one side of a membrane to
activity the other.
GO:0015293 symporter activity Enables the active transport of a solute
across a membrane by a mechanism
 whereby two or more species are
transported together in the same direction
in a tightly coupled process not directly
linked to a form of energy other than
chemiosmotic energy.
GO:0015377 cation:chloride Enables the transfer of a solute or solutes
symporter activity from one side of a membrane to the other
according to the reaction: cation(out) +
Cl-(out) = cation(in) + Cl-(in).
GO:0015378 sodium:chloride Enables the transfer of a solute or solutes
symporter activity from one side of a membrane to the other
according to the reaction: Na+(out) + Cl-
(out) = Na+(in) + Cl-(in).
GO:0015379 potassium:chloride Enables the transfer of a solute or solutes
symporter activity from one side of a membrane to the other
according to the reaction: K+(out) + Cl-
(out) = K+(in) + Cl-(in).
GO:0022857 transmembrane Enables the transfer of a substance,
transporter activity usually a specific substance or a group of
related substances, from one side of a
membrane to the other.

Phenotypes, diseases and traits associated with this gene SLC12A3

Phenotype, disease and trait Source

GITELMAN SYNDROME MIM morbid, Orphanet

Phenotype, disease and trait annotations associated with variants in this gene
Phenotype, disease and Source(s) Number of variants
trait
Familial hypokalemia- ClinVar 141
hypomagnesemia
Muscle weakness ClinVar 2
HDL cholesterol NHGRI-EBI 2
GWAS catalog
HDL cholesterol levels NHGRI-EBI 1
GWAS catalog
ClinVar: phenotype not ClinVar 62
specified
Phoneme awareness NHGRI-EBI 1
GWAS catalog
 Phenotype, disease and Source(s) Number of variants
trait
ALL variants with a
phenotype annotation
- 574
Annotated by HGMD but no HGMD- 399
phenotype description is publicly PUBLIC
available

Annotated by HGMD but no phenotype description is publicly available

Codul SNP: rs2304479

Alleles: A/G
Ancestral form: A
MAF:  0.12(G) Highest population MAF: 0.34
Phenotype data: Familial hypokalemia-hypomagnesemia
 TABLE: Alelle frequency
A G
EUR * *
CEU 95,5% 4,5%
FIN 96% 4%
GBR 94% 6%
IBS 93,5% 6,5%
TSI 95,3% 4,7%

TABLE: Genotype frequency

AA AG GG
EUR * *
CEU 91,9% 7,1% 1%
FIN 91,9% 8,1% %
GBR 87,9% 12,1% %
IBS 86,9% 13,1% %
TSI 90,7% 9,3% %
Task3

În contextul respectiv pot să evidențiez faptul că bioinformatica se practică precum o disciplină înrudită cu
informatica medical, dedicată stocării şi prelucrării datelor din biologie. În ultimele decenii, aceste date au
început să conţină tot mai multe detalii structurale, în special secvenţe moleculare. Informaţiile noi, care
sosesc cu o cadenţă accelerată impun cu stringenţă nu numai noi abordări în organizarea şi sistematizarea
datelor şi algoritmi performanţi de prelucrare, ci şi necesitatea pregătirii unor specialişti în bioinformatică.
Aplicaţiile din ce în ce mai numeroase în domeniul medical şi farmaceutic au ridicat şi mai mult presiunea,
astfel încât în ultimul deceniu al secolului trecut au apărut primele calificări, la nivel masteral, în
bioinformatică.

Trebuie însă remarcat caracterul interdisciplinar deosebit al bioinformaticii, acoperind o paletă mult mai
largă decât alte discipline de graniţă. Tendinţa de îngustare a domeniului de specializare, manifestată
pregnant în învăţământul superior în ultima parte a secolului XX a redus sensibil aria de recrutare a
specialiştilor în toate ramurile multidisciplinare, iar pentru bioinformatică această reducere a devenit
drastică. Comunitatea specialiştilor bioinformaticieni, deşi într-o rapidă creştere, este încă destul de redusă –
probabil aşa se explică de ce această specialitate este cea mai bine remunerată dintre toate specialităţile
informaticii medicale.

Ce este deosebit la această interdisciplinaritate? Aşa cum am afirmat mai sus - paleta largă de intersecţie a
unor discipline, uneori destul de îndepărtate între ele. Într-adevăr, centrul atenţiei se îndreaptă spre
principalele molecule de interes informaţional: acizi nucleici şi proteine.

Deci este necesară o bază solidă de biologie moleculară, care este ea însăşi un domeniu interdisciplinar,
necesitând o serie de cunoştinţe de biochimie şi de biologie celulară. Pentru aplicaţiile medicale şi
interpretarea hărţilor cromozomiale sunt necesare o serie de cunoştinţe de genetică. Să ne îndreptăm privirea
şi în cealaltă parte, privind dezvoltarea metodelor de prelucrare. Sunt necesare cunoştinţele solide de teoria
probabilităţilor şi statistică, topologie sau analiza seriilor. Determinarea structurilor tridimensionale şi a
aplicaţiilor farmaceutice de “drug design” sunt aplicaţii ale chimiei cuantice. O serie de noţiuni de fizică şi
biofizică sunt de asemenea necesare, atât pentru înţelegerea proprietăţilor structurilor analizate cât şi a
metodelor utilizate pentru obţinerea şi interpretarea datelor experimentale.
Să nu uităm nici capitolul de biologia sistemelor, în care sunt urmărite procesele de semnalizare celulară,
studiate de fiziologia celulară şi prelucrate prin simulare atât prin ecuaţii diferenţiale cât şi simulare
stochastică. Pentru viziunea integrativă sunt necesare noţiuni din teoria sistemelor, cibernetică şi teoria
evoluţionismului. Desigur, să adăugăm în final şi câteva discipline fundamentale din ştiinţa calculatoarelor –
baze de date şi limbaje de programare.

Referitor la informația genomică în lucrul cu pacienții este evident faptul că Progresele recente în ingineria
genomică vor permite modificarea secvențelor de ADN în celulele vii, făcând posibil controlul materialului
genetic într-un mod fără precedent. Pentru a asigura un conses al acestor măsuri, este primordial
profesionalismul către această disciplină.

Din punt de vedere profesional îngrijirea pacientului este, de obicei, un lucru de echipa şi accesul la
informațiile despre pacient este esențial pentru siguranța pacientului și continuitatea ingrijirii lui. Cei mai
mulţi pacienţi sunt conștienți de faptul că informațiile despre ei trebuie să fie împărtășite între profesioniștii
din domeniul sănătății care furnizează servicii de ingrijire medicală, respectiv este primordială stabilirea
unei relații medic- pacient eficientă.

In contextul dezvoltării bioinformaticii la noi în republică, am calificat un șir de puncte slabe. Acestea redau
insuficiența personalului medical, lipsa profilului genetic al populației, precum și lipsa siguranței și calității
produselor (echipament medical, medicamente, alimente). Actual putem observa o colaborare intersectorială
insuficientă și o finanțare insuficienta a serviciilor medicale. Calificarea insuficientă a personalului în
implementarea noilor tehnologii, în concordanță cu finanțare insuficientă, coordonarea cercetării este sub
nivel și colaborarea intersectorială și interdisciplinară este insuficientă. Actual încă nu este pusă la punct fișa
electronică a pacientului, nu este finalizată baza legislative, predomină astfel lipsa unui suport legislative, a
cadrului legal de management al datelor medicale și lipsa bazei de date medicale personalizate la nivel
național