APELLIDOS NOMBRES CURSO JV

MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question.

1) Applications of mass spectrometry include:

A) determining the primary structure of proteins.
B) detection of post-translational modifications on proteins.
C) determination of the mass of a protein.
D) A and B.
E) A, B, and C.

2) In digestion, proteins are broken down into amino acids by a(n) ________ reaction.
A) denaturation
B) saponification
C) hydrolysis
D) oxidation
E) reduction

3) What process occurs when heat, acids, bases, and heavy metal ions cause a loss of biological function of a
protein?
A) hydrogenation
B) saponification
C) denaturation
D) esterification
E) amidation

4) Hydrophobic interactions help to stabilize the ________ structure(s) of a protein.

A) secondary and tertiary
B) secondary and quaternary
C) secondary
D) tertiary and quaternary
E) primary

5) Collagen, a protein found in tendons and cartilage, would be classified as a ________ protein.
A) structural B) catalytic C) storage D) hormone E) transport

6) Wool is primarily made up of

A) enkephalin.
B) protein.
C) triacylglycerols.
D) globin.
E) carbohydrate.

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7) Sucrase, the protein that facilitates the hydrolysis of sucrose, would be classified as a ________ protein.
A) transport B) hormonal C) contractile D) catalytic E) structural

8) The structural formulas of amino acids are the same EXCEPT for the
A) alpha carbon.
B) side (R) group.
C) carboxyl group.
D) amino group.
E) hydrogen bonding.

9) A completely vegetarian diet will contain all the essential amino acids if it includes
A) corn and beans.
B) almonds and walnuts.
C) wheat and corn.
D) wheat and rice.
E) rice and beans.

10) Which of the following is the correct structure for Ser-Ala-Asp? The appropriate side chains look like this.
O

Ala: -CH 3 ; Ser: -CH 2 OH; Asp: -CH2 COH

A) COOH OH
| |
CH2 O CH2 O CH 3 O
| | |
H3 N C H - C - NH - C H - C - NH - C H - C O -
+

B) COOH OH
| |
CH2 O CH3 O CH 2 O
| | |
H3 N C H - C - NH - C H - C - NH - C H - C O -
+

C) OH COOH
| |
CH2 O CH2 O CH 3 O
| | |
H3 N+ C H - C - NH - C H - C - NH - C H - C O -

D) OH COOH
| |
CH3 O CH2 O CH 2 O
| | |
H3 N+ C H - C - NH - C H - C - NH - C H - C O -

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E) OH COO-
| |
CH2 O CH3 O CH 2 O
| | |
H3 N C H - C - NH - C H - C - NH - C H - C O -
+

11) Denaturation of a protein

A) hydrolyzes peptide bonds.
B) can only occur in a protein with quaternary structure.
C) disrupts the secondary, tertiary, or quaternary structure of a protein.
D) is always irreversible.
E) changes the primary structure of a protein.

12) Acids and bases denature a protein by disrupting

A) hydrophobic interactions and peptide bonds.
B) ionic bonds and hydrogen bonds.
C) amide bonds and alkene bonds.
D) ionic bonds and hydrophobic interactions.
E) peptide bonds and ionic bonds.

13) Disulfide bonds in a protein chain connect

A) an amine and a carboxylic acid group.
B) an alcohol and a carboxylic acid group.
C) two cysteine residues.
D) two asparagine residues.
E) tryptophan and alanine residues.

14) The fibrous protein responsible for the structure of hair and wool is
A) keratin. B) collagen. C) myosin. D) casein. E) endorphin.

15) In the peptide Ala-Try-Gly-Phe, the N-terminal amino acid is

A) glycine.
B) alanine.
C) phenylalanine.
D) aspartic acid.
E) tryptophan.

16) In the peptide Ser-Cys-Ala-Gly, the C-terminal end is

A) glycine. B) serine. C) glycerine. D) alanine. E) serotonin.

17) Consider a mixture of the amino acids lysine (pI = 9.7), tyrosine (pI = 5.7), and glutamic acid (pI = 3.2) at a pH
5.7 that is subjected to an electric current. ________ will migrate towards the positive electrode(+).
A) Tyrosine B) Glutamic acid
C) Lysine D) All of the amino acids

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 APELLIDOS NOMBRES CURSO JV

18) Consider a mixture of the amino acids lysine (pI = 9.7), tyrosine (pI = 5.7), and glutamic acid (pI = 3.2) at a pH
5.7 that is subjected to an electric current. ________ will remain stationary.
A) Lysine B) Glutamic acid
C) Tyrosine D) All of the amino acids

19) A basic amino acid has an R group that contains

A) a methyl group.
B) a thiol group.
C) a carboxyl group.
D) an amine group.
E) an alcohol group.

20) Glycine is the only naturally occurring amino acid that is

A) neutral.
B) positively charged.
C) achiral.
D) negatively charged.
E) in the L- form.

21) Zwitterions have an overall positive charge.

A) True
B) False

22) Insulin is a transport protein.

A) True
B) False

23) Hemoglobin is a transport protein.

A) True
B) False

24) Protein biosynthesis uses only L-amino acids.

A) True
B) False

MATCHING. Choose the item in column 2 that best matches each item in column 1.

Identify the structural level in each protein.

25) The protein folds into a compact A) tertiary

structure stabilized by interactions
between R groups.

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26) the combination of two or more A) secondary structure

protein molecules to form an active
protein B) quaternary

27) pleated sheet C) primary structure

28) the peptide bonds between the amino

acids

Classify each protein by function.

29) actin in muscle A) structural

30) antibodies B) contractile

31) collagen in tendons and cartilage C) transport

32) lipoproteins in the blood D) catalytic

33) trypsin for the hydrolysis of protein E) protection

Would an amino acid with the given R group be most likely to be found in the hydrophobic or hydrophilic region of a protein?

34) -CH 2 - CH - CH 3 A) both

|
CH3 B) hydrophilic

C) hydrophobic
35) - CH - CH 3
|
OH

36) - CH 3

37) -CH 2 CH2 SCH3

38) O

-CH 2 - C - NH2

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39) A) hydrophilic

B) hydrophobic

40)

41) -CH - CH 2 - CH 3
|
CH3

42)

SHORT ANSWER. Write the word or phrase that best completes each statement or answers the question.

43) At physiological pH, the carboxylic acid group of an amino acid will be ________, while the amino group will
be ________, yielding the zwitterion form.