Agilent’s New 7000B

Quadrupole GC/MS/MS:
Evolving technology for
higher performance tools

Terry L. Sheehan, Ph.D.

GC/MS/MS Product Manager
Agilent Technologies
Santa Clara, CA

Page 1
 Triple Quadrupole MS/MS
(also called tandem quadrupole)
is changing the way scientists
think about GC/MS

Page 2
Transmission Quadrupole MS & MS/MS
Sensitivity and Selectivity Scale
Least Sensitive Most Sensitive

<
Fast 12,500 u/s (5975C) Typical < 2,000 u/s
< Dwell 20-50 ms

Fast 6,250 u/s (7000) Targets & Non-targets Dwell 1-50 ms

Targets & Non-targets Targets only
*Multiple Reaction
Monitoring
Also called SRM for
Selected Reaction
Monitoring (similar to
SIM – Selected Ion
Monitoring)

Page 3
Transmission Quadrupole MS & MS/MS
Sensitivity and Selectivity Scale
Least Sensitive Most Sensitive

<
Fast 12,500 u/s (5975C) Typical < 2,000 u/s
< Dwell 20-50 ms

Fast 6,250 u/s (7000) Targets & Non-targets Dwell 1-50 ms

Targets & Non-targets Targets only

Least Selective Most Selective

Unit mass resolution

< Unit mass + AMDIS
deconvolution
<
Targets only per DBL

Page 4
Summary of Relative Performance Factors
GC/MSD GC/MSD GC/MSD GC/TQ
Scan SIM Scan-DRS MS/MS
S from analyte + +++ + +++
S from matrix -- -- - 0
N from neutrals = = = Ultra low
N from matrix --- --- - 0
N from "bleed" Low Low Lower 0
MDL (clean) Very Low Lowest Lower Lowest
MDL (very dirty) Low Low Lower Lowest
Quant Error Low Low Lower Lowest
Sanalyte
MDL = But scan is
Nneutral + Nmatrix + NGC “bleed”
essential for
Smatrix non-targets
Quant Error = (unknowns)!
Sanalyte + Smatrix

Page 5
Fundamental Benefits of MS/MS for GC

Higher Selectivity to Identify

Target Analytes in Complex Matrices

Consistently High Sensitivity for

Target Analytes in Complex Matrices

Page 6
MS/MS Eliminates Scan and SIM Interferences

Single Quad MS
selectivity proportional to
spectral resolution
no selectivity against ions interference
with same m/z
analyte

unit mass resolution

Page 7
MS/MS Eliminates Scan and SIM Interferences

Single Quad MS
selectivity proportional to
spectral resolution
no selectivity against ions interference
with same m/z
analyte
Product 2

Product 1
Product 3

unit mass resolution

analyte

Product Ion Spectrum

Page 8
MS/MS Eliminates Scan and SIM Interferences

Single Quad MS
Triple Quad MS
selectivity proportional to
High probability that one or more of the
spectral resolution
product ions will be a unique dissociation
no selectivity against ions interference
product of the precursor only
with same m/z
analyte
Product 2

Product 1 interference
Product 3

unit mass resolution

analyte

Product Ion Spectrum

Page 9
MS/MS Ensures Lowest Detection Limits
EI: spectrum of analyte can also include
ions from matrix, column bleed, gases, etc.

isolate precursor
Q1 SIM before CID

chemical noise
from these other
ions is eliminated

Page 10
MS/MS Ensures Lowest Detection Limits
EI: spectrum of analyte can also include
ions from matrix, column bleed, gases, etc.

Product 2

Product 1
isolate precursor Product 3
Q1 SIM before CID

chemical noise
from these other CID + Lower m/z Product

ions is eliminated Q2 SIM Ions measured against

zero chemical noise
for consistently lower
LOQ and LODs

Page 11
 SIM Limited by Unit Mass Resolution

GC/MS Single Quad SIM

in diesel fuel

Interfering matrix
peaks = chemical
noise

57 284

C20 Eicosane
100 100

Hexachlorobenzene
43
Cl

Cl Cl

71

50 50
Cl Cl
142
Cl
85

107 249

29 214

99 71 177
113 127 118
141 155 169 183 197 211 225 282 47
95
238 253 35 83 130
0 24 53 153
165 190 237
20 40 60 80 100 120 140 160 180 200 220 240 260 280 0
20 40 60 80 100 120 140 160 180 200 220 240 260 280 300
(ma inlib ) Eic osa ne (ma inlib ) Benzene, hexa c hloro-

Page 12
 SIM Limited by Unit Mass Resolution

GC/MS Single Quad SIM

in diesel fuel

Interfering matrix
peaks = chemical
noise

57 284
100 100

43
Cl

Cl Cl

71

50 50
Cl Cl
142
Cl
85

107 249

29 214

99 71 177
113 127 118
141 155 169 183 197 211 225 282 47
95
238 253 35 83 130
0 24 53 153
165 190 237
20 40 60 80 100 120 140 160 180 200 220 240 260 280 0
20 40 60 80 100 120 140 160 180 200 220 240 260 280 300
(ma inlib ) Eic osa ne (ma inlib ) Benzene, hexa c hloro-

Page 13
SIM Limited by Unit Mass Resolution

GC/MS Single Quad SIM

in diesel fuel

Interfering matrix
peaks = chemical
noise

At unit mass resolution SIM,

HCB is buried by the more
intense peaks from C20 to
C22 hydrocarbons

Page 14
MS/MS Succeeds Where MS Fails

GC/MS Single Quad SIM

in diesel fuel

Interfering matrix
peaks = chemical
noise

GC/MS Triple Quad SRM

A chromatographer’s
dream: single peak on
flat baseline

Page 15
Pesticide Analysis: Comparing SIM to MS/MS
SIM ‐ EIC (246)

9 Apple

9 Cabbage

9 Ginseng

X Orange

X Spinach

Page 16
Pesticide Analysis: Comparing SIM to MS/MS
SIM ‐ EIC (246) MRM (2 transitions)
2 4 6 . 0 -> 1 7 6 . 1 , 2 4 6 . 0 -> 1 7 5 . 1

Counts
x1 0 4 R a t io = 2 3 . 5

9
1 .1
1
0 .9
0 .8
0 .7

Apple
0 .6
0 .5
0 .4
0 .3
0 .2
S/N = 448
0 .1
0
-0 . 1
1 1 .8 1 1 .9 12 1 2 .1 1 2 .2 1 2 .3
A c q u is it io n T im e (m in )
2 4 6 . 0 -> 1 7 6 . 1 , 2 4 6 . 0 -> 1 7 5 . 1

9 9

Counts
x1 0 3 R a t io = 2 2 .9

Cabbage 4

1
S/N = 241
0

1 1 .8 1 1 .9 12 1 2 .1 1 2 .2 1 2 .3
A c q u is it io n T im e (m in )
246.0 -> 176.1 , 246.0 -> 175.1

9 9

Counts
x10 4 Ratio=23.3
0.9

0.8

0.7

0.6

Ginseng 0.5

0.4

0.3
S/N = 446
0.2

0.1
0

9
11.8 11.9 12 12.1 12.2 12.3
Ac quisition Time (min)

X
2 4 6 . 0 -> 1 7 6 . 1 , 2 4 6 . 0 -> 1 7 5 . 1

Counts
x1 0 3 R a t io = 2 3 .6
8

Orange 6

4
S/N = 456
3

9
1 1 .8 1 1 .9 12 1 2 .1 1 2 .2 1 2 .3
A c q u isitio n T im e (m in )
2 4 6 . 0 -> 1 7 6 . 1 , 2 4 6 . 0 -> 1 7 5 . 1

Counts
x1 0 4 R a t io = 2 4 . 0

X
1 .2
1 .1
1

S/N = 260
0 .9

Spinach 0 .8
0 .7
0 .6
0 .5
0 .4
0 .3
0 .2
0 .1
0
-0 . 1
1 1 .8 1 1 .9 12 1 2 .1 1 2 .2 1 2 .3
A c q u is it io n T im e (m in )

Page 17
7000B MS/MS: High Performance Components
The best technologies from the
industry leading 5975C Series Gold plated quartz, hyperbolic
quadrupoles
GC/MSD
New inert,
– Heated monolithic gold high temp,
plated quartz quadrupole extraction EI
source
– Reliable, inert, high
performance ion source
– High Signal/Low Noise New Ramped Iris
Triple-Axis Detector Triple-Axis Detector

– PCI/NCI ion source

High pressure hexapole collision
cell with Patent Pending Helium
Quench Gas
…and the 6400 Series
LC/MS/MS
Split Flow Turbo
– Linear acceleration enhanced
Hinged access to source
hexapole collision cell and detector
– MassHunter software

Page 18
7000B MS/MS: Hot (200ºC) Quartz Analyzers
High temperature Gold plated quartz, hyperbolic
analyzers stay clean quadrupoles

even with highly

concentrated,
complex matrices

Page 19
Proprietary Heated Quartz Quadrupole

• High temperature (200ºC)

operation results in greater
reliability with “dirty” samples
– MS/MS methods typically have more
“dirty” samples and benefit even more
from the higher temp quad analyzer
– Virtually eliminates the need to clean
quads as a maintenance issue
• More stable tunes and methods
over a longer period of time in real
world sample environments

Page 20
Hyperbolic, Gold-coated, Quartz - Proprietary!

• Why quartz?
– Low coefficient of linear expansion (expansivity; α)
• dL = α L0 dt [t = ºC; dL = change in length (m); L0 = length at 0 ºC]
– α varies greatly for different materials (m/m K x 10-6)
• Stainless steel 304 = 17.3; Stainless steel 316 = 16.0
• Inconel (Ni alloy) = 12.6; Monel (Ni/Cu alloy) = 13.5
• Glass pyrex = 4.0; Quartz, fused = 0.59
– For change from 25 to 150 ºC, which material expands the LEAST?
• But what is the “analytical value?”
• Low thermal expansion means dimensions/alignments do not change
• After maintenance, the quartz quad can be baked at 200 ºC
• Under operation, the quartz quad can be run at 150, 180 or even 200 ºC
• Heat is the “friend” of GC/MS methods – true for the source and analyzer!

Page 21
More Information on the Agilent Website

Detailed Video

Page 22
7000B MS/MS: Collision Cell with Helium Quench
Highly efficient
dissociation of
precursor ions

Maximum transitions
per second without
product ion
carryover (cross-talk) High pressure hexapole collision
cell with Patent Pending Helium
Quench Gas

Reduction of
metastable helium
from EI source

Page 23
Hexapole Cell: Better Transmission Characteristics

Mass Range Transmission

Quadrupole Hexapole Octapole

1.2
1
0.8
0.6
0.4
0.2
sn
om
ra
iT

GC/MS
m/z=1050
0
0 500 1000 1500
m/z

Quadrupoles are the best mass filters (analyzers)

Hexapoles and octapole are the best transmission devices

Page 24
500 Transitions/sec: Why Is This Important?

• Narrow GC peaks (width <1-2 s)

• Sufficient data points (15) to perform acceptable/accurate
quantification
• Many compounds to monitor in single run (multiresidue)
when coeluting peaks are quite frequent
• Regulated environment where QC checks are required to
ensure data validity
– Confirmatory transitions according to the 96/23/CE directive

Page 25
Measured Collision Cell Clearing Time

1 msec

No product ion signal =

no cross-talk at 500
transitions per second

Page 26
Collision Cell Process: Typical Description

Collision Cell
1 ml/min N2
Collision Gas

Quad Analyzer Quad Analyzer

Precursor Product
Source Detector
Ions In collision induced dissociation Ions Out

This is a good description for LC/MS/MS,

but it is not complete for GC/MS/MS

Page 27
Collision Cell Process: Full Description for GC/MS

Collision Cell
1 ml/min N2 At the detector,
Collision Gas metastable
helium generates
neutral noise.
Quad Analyzer Quad Analyzer

Precursor
Source He* +
Ions In
A high population of highly
energetic helium
metastables are produced
in an EI source; since
metastable helium is not
charged, it can pass
through mass analyzer
field into the collision cell
and through to the HED-EM
collision induced dissociation
Product
+ He* Detector
Ions Out
In GC/MS, neutral noise is
buried in much higher
chemical noise.
In GC/MS/MS, chemical
noise is greatly reduced
so neutral noise is a
critical source of noise.

Page 28
Collision Cell Process with Helium Quench Gas

Collision Cell
1 ml/min N2
Collision Gas

Quad Analyzer Quad Analyzer

Precursor Product
Source He* + + He* Detector
Ions In collision induced dissociation Ions Out
Transmission of
metastable helium
to the detector is
greatly reduced;
He Buffer Gas the Triple-Axis
detector further
reduces neutral
He* + He → 2 He + heat noise for ultra-low
neutral noise.

Page 29
 Benefit from “Helium Quench”
+MRM(283.79999->213.90039) 1pgHCB_std_cond_UnkGain_2090EMV_He0pct_05.d
x103 Noise(RMS) =20.08; SNR(6.470min) =341.0
tested with
7.5 Same hexachlorobenzene
6.470
7
6.5
Signal
6
5.5
Noise Decreased
5

Helium Quench Gas OFF

+M RM(283.79999->213.90039)1pgHCB_std_cond_UnkG
ain_2090EM
V_He0pct_05.d
4.5 x102 Noise(RMS)=20.08;SNR(6.470m in)=341.0
2
4 1.9

3.5 1.8
1.7
3
1.6
2.5 1.5

2
1.5
S/N 341:1 1.4
1.3
1.2
1 1.1

0.5 4.628
1
6.301
0.9
0
0.8
4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 5 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 5.9 6 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9
0.7
Countsvs. AcquisitionTime(min)
0.6

0.5
+MRM(283.79999->213.90039) 1pgHCB_std_cond_UnkGain_2090EMV_He20pct_04.d 4.7 4.75 4.8 4.85 4.9 4.95 5 5.05 5.1 5.15 5.2 5.25 5.3 5.35 5.4 5.45 5.5 5.55 5.6 5.65 5.7 5.75 5.8 5.85 5.9 5.95 6 6.05 6.1 6.15 6.2
x103 Noise(RMS) =6.03; SNR(6.465min) =1294.0 Countsvs.AcquisitionTim
e(m
in)

8 Same 6.465
7.5
Signal
7
6.5
In MS apps, neutral noise
6
5.5
is not visible – it is buried
5
by more intense chemical
4.5
Helium Quench Gas ON
4
3.5
noise.
3
2.5 Since MS/MS eliminates
2
1.5
S/N 1294:1 chemical noise, neutral
1
0.5 noise is visible and must
be minimized.
0

4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 5 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 5.9 6 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 7
Countsvs. AcquisitionTime(min)

Page 30
7000B MS/MS: New Higher Sensitivity Source
Increase signal for
precursor ions using
an extractor source New inert,
high temp,

design extraction EI
source

Page 31
Lab-Proven Inert Source

• Solid, inert materials, not a

coating that can be damaged by
cleaning
• Stay-Clean (350ºC) design
extends maintenance intervals with
dirty samples
• Source tune parameters in the
Autotune file
• Dual filaments reduces
maintenance intervals
Partition between source and
• 1000’s of satisfied MSD users analyzer compartments
support dual stage pumping

Page 32
 7000B Extraction Source
External View - Assembled

Like the 7000A source, the 7000B

extraction source is more open than the
MSD source – looks similar to the CI
source

At first look, it does not look any

different than the 7000A source

Page 33
 7000B Extraction Source
Expanded View of Components

Component #7 is a new
insulator

And there is new

connection to the
lens #11

Design uses
combination of
“push” (repeller) and
“pull” (extractor lens)
to increase ion
intensity

Page 34
 7000B Increased Signal

Extractor Source w/
Extractor source Ramped Iris Detector
cutaway

Push-Pull Principle:
Standard Source
Repeller and Extractor
& Detector mode

Page 35
 Why Is the New Extractor
Source More Valuable for
Triple Quad MS/MS
than
Single Quad MSD?

Page 36
Extractor Source Increases Signal for All Ions
EI: spectrum of analyte can also include
ions from matrix, column bleed, gases, etc.

Single Quad MS
Chemical Noise Limited
Analyte Signal Increased
Chemical Noise Increased
S/N does not improve

Page 37
Extractor Source Increases Signal for All Ions
EI: spectrum of analyte can also include
ions from matrix, column bleed, gases, etc.
MS/MS still eliminates
chemical noise

Product 2

Product 1
isolate precursor Product 3
Q1 SIM before CID

chemical noise
from these other CID +
ions is eliminated Q2 SIM

Page 38
Why Is the New Extractor Source Valuable for
MS/MS?

Single Quad MS
Chemical Noise Limited
Analyte Signal Increased
Chemical Noise Increased
S/N does not improve

Triple Quad MS/MS

Product Ion Signal Limited
Product Ion Signal Increased
Chemical Noise Does NOT Increase
S/N (and LOD) are improved

Page 39
Unique Entrance Lens Eliminates Pre-filter

Improved lens

The last lens, which extends into the heated monolithic quadrupole,
optimizes coupling of the source to the analyzer. Voltage on this lens is
dynamically ramped synchronously with analyzer voltage to focus
maximum ion signal for every m/z into the center of the quadrupole field.

Page 40
7000B MS/MS: New Ramped-Iris Detector
Ramped-iris
improves transfer of
ion from the exit of
the second analyzer
into the Triple-Axis
detector New Ramped Iris
Triple-Axis Detector

Page 41
High Performance Triple-Axis Detector with New
Dynamically Ramped Iris
• Maximum reduction of neutral
Offset
noise shield for
Z
steering rod secondary
– The combination of helium quench hyperbolic quartz
particles

gas and the Triple-Axis design transmission

quadrupole
high energy
dynode
delivers ultra low noise analyzer

• Long life and high linearity

– Triple channel electron multiplier Y
• “Gain Normalization” corrects tune triple channel
electron
ion beam
file for detector aging X
multiplier

– Consistent long term method

sensitivity
• Design proven in the 5975C MSD

Page 42
 Ramped-Iris Triple-Axis Detector
Mass Iris (V) Signal
Increase
• Improved high mass peak shape
69 -1 1.0X
• Improved mid to high mass sensitivity
131 -8 1.1X

264 -13 1.5X

414 -20 2.5X

502 -30 2.8X

614 -35 4.0X

Page 43
7000B GC/MS/MS Specifications

Installation Checkout Specifications

EI SRM > 500:1 for 100 fg OFN
PCI SRM (CH4) > 50:1 for 100 fg BZP
Typical Precision
EI MRM Pk Area RSD < 8% for 100 fg OFN
Typical Sensitivity
EI Scan > 300:1 for 1 pg OFN
NCI SIM (CH4) > 200:1 for 10 fg OFN
OFN = octafluoronaphthalene
BZP = benzophenone

Page 44
 Column
Backflush:
Many Wonderful
Advantages for
GC/MS/MS of
Complex Matrices

Page 45
 What Real Value Does High Performance EPC Have If
Matrix Carryover Destroys Rt Precision?
N or m .

140 00
10 Runs without Backflushing
120 00 Retention times shift ~4-5 sec
100 00 during 10 runs
80 00

60 00

40 00

20 00

5 .3 5 .4 5 .5 5 .6 5 .7 5 .8 5 .9 6 6 .1 6 . 2 m in

N or m .

160 00

140 00
10 Runs with Backflushing
120 00

100 00

80 00

60 00

40 00

20 00

5 .3 5 .4 5 .5 5 .6 5 .7 5 .8 5 .9 6 6 .1 m in

Page 46
Productivity: CFT Backflush

• More analyses per hour . . .

– Shorter analysis cycle time (time from injection to injection)
– Reduced requirement for sample preparation
• with consistently better results . . .
– Quantitative: accuracy, precision, detection limits, linear range
– Qualitative: response ratios, confirmatory retention times, library
searches (eliminate false negatives and false positives)
• while using less operator time for each analysis.
– Less time performing maintenance on column and ion source

Page 47
Multiple Backflush Configurations
Multiple Backflush Devices
• Backflush from the start of the column
– Backflush guard column only
• Backflush from the end of the column
– Backflush entire column
• Backflush from the middle of the column
– Backflush a percentage of the column
– Example: at tee between two 15 m columns
Purged
Ultimate
New Pressure Union
Controlled
Tee Kit

Page 48
Backflush – Mid-Column (Backflush Mode)
Split/Splitless Pressure /
Injection Port Flow
Controller

Vent
Decrease inlet
7890A GC pressure during Pressure
backflush Controlled Tee

5975C MSD or
7000B MS/MS

15-m HP-5ms 15-m HP-5ms

(0.25mm id x0.25um) (0.25mm id x0.25um)

Page 49
Backflush with Purged Ultimate Union: Lipid
Peroxidation Products in Blood
4-hydroxy-2,3-nonenal (HNE), an indicator
of oxidative stress, and its metabolite, 1,4-
dihydroxynonene (DHN)

PCI with NH3

Late eluting
peaks were
quickly
contaminating
the source

App Note 5989-9359EN

Page 50
Backflush with Purged Ultimate Union: Loss of
Response without Backflush

with backflush

without backflush

App Note 5989-9359EN

Page 51
Backflush with Purged Ultimate Union: Loss of
Response without Backflush

with backflush

These were the first

backflush results; the
system is getting clearer

without backflush

App Note 5989-9359EN

Page 52
 Break for
Questions

For questions, at break

please dial *1 on your
phone, or type onto the
Q&A box at any time during
the presentation.

Pesticide Analysis with New Agilent

Page 53
Jet Stream Technology
December 12, 2008
Page 53
 MassHunter
Software

Page 54
MassHunter Software
Common Workflow for all Mass Spec. applications

ChemStation Translator
Single Quad LC/MS
MassHunter
ChemStation Translator
Single Quad GC/MS (MSD) Quant
and

Qual
LC/MS Acquisition Module modules
Triple Quad LC/MS, LC/TOF, LC/QTOF
MassHunter format

GC/MS Acquisition Module

Triple Quad GC/MS

Page 55
MassHunter Data Analysis Applications
One software for all your Agilent MS applications

•Quantitative Analysis •Qualitative Analysis

•Target Compound Analysis •Discovery & Method Development
• High throughput quantitation • Find features
• Identify compounds

Page 56
Quantitative Analysis View

Results Information

Page 57
Quantitative Analysis View

Results Information

Compound Information

Page 58
Quantitative Analysis View

Results Information

Compound Information
Calibration Information

Page 59
Results Information:
All Results for batch in one view

• Easy visualization for all results

– Customize columns for desired data
• Easy Navigation
– Compound and Calibration views change when new compound is selected
– Reduce data by filtering on sample type, compound, time segment, and outlier

Page 60
Results Information:
Detailed compound view

•Detailed compound information is easily seen

Page 61
Results Information:
Highlight outliers

•Compounds with results outside specified

ranges are highlighted
• 30 metrics are available
• Custom Calculation is possible
• More to be added
– i.e. Peak metrics

Page 62
Results Information:
Highlight outliers

•Compounds with results outside specified

ranges are highlighted
• 30 metrics are available
• Custom Calculation is possible
• More to be added
– i.e. Peak metrics

Page 63
Results Information :
Simple Metrics – Plot Columns

•Ability to see trends in a batch

•Ability to diagnose quantitation
problems easily
• Missing ISTD amount
• Retention time drift

Page 64
Compound Information:
All relevant information in one view

Compound Qualifier Spectrum

Integration Information Information

ISTD ISTD Qualifier ISTD Spectrum

Integration Information Information

Page 65
Calibration Curve
Views

QC point
Can include ISTD, QC,
and CC data points on
curve
Point information is
available as pop-up

Page 66
Calibration Curve
Assistant

Display all possible

Calibration Curve fit options
•Review R2, Standard error,
or Max % Residual
•Can sort, filter, or browse
options

Page 67
Calibration Curve
Assistant

Filter possible calibration

curves based on
requirements

Compare selected
calibration fit (black) with
proposed (blue).
•Linear
•All points
•Ignore origin

Page 68
Reporting and Data Transfer

Use of XML results format

• Easy data exchange
– LIMS data exchange
– Copy/Paste views from MassHunter
Microsoft Excel reporting engine
• Powerful Features
– Excel Macros and formulas can be
created for custom result
manipulation
• Flexible Reports and Layouts
– Custom Layouts and graphics
properties.
• Preconfigured report template
examples

Page 69
Productivity of GC/MS/MS

• Sample complexity forces the change from MS to MS/MS

– Could not meet required LOD/LOQ in real samples
– Could not avoid interferences from matrix ions (isobaric ions)

Page 70
Productivity of GC/MS/MS

• Sample complexity forces the change from MS to MS/MS

– Could not meet required LOD/LOQ in real samples
– Could not avoid interferences from matrix ions (isobaric ions)
• Practical aspects of productivity
– MS/MS selectivity for less sample preparation
– MS/MS sensitivity for less injected on-column
• In turn, less maintenance
– MS/MS selectivity for faster analysis cycles
• MS/MS selectivity lessens the requirement for column selectivity
• Less bakeout or backflush may be needed
– Caution: selectivity is a type of ‘blindness’ and can be misleading
– MS/MS selectivity for faster and easier peak integration
• What can be easier than a flat baseline and a single peak

Page 71
 Break for
Questions

For questions, at break

please dial *1 on your
phone, or type onto the
Q&A box at any time during
the presentation.

Pesticide Analysis with New Agilent

Page 72
Jet Stream Technology
December 12, 2008
Page 72
Thank You for Attending Today’s eSeminar

Terry L. Sheehan, Ph.D.

GC/MS/MS Product Manager
Agilent Technologies, Santa Clara, CA

Page 73