Question 7

The lineweaver burk plot for the given values is shown below,

1/ [S]
 0.1
 0.066667
 0.05
 0.04
 0.033333
 0.022222
 0.016667

1/Rate
 1.666667
 1.333333
 1.111111
 1
 0.909091
 0.833333
 0.8

The equation for lineweaver burk plot is,

This equation resembles straight line equation Y=mX + C, where, m is the

slope and c is the y-intercept.

The y-intercept in the above plot is given by 1/Vmax.

From the above plot y-intercept =0.586.

The slope in the above plot is given by Km/Vmax.

From the above plot slope =10.78

Question 8

The lineweaver burk plot for the given values of inhibited enzyme rate 1
is shown below,

1/ [S] 1/ Rate 1
 0.2  10
 0.1  5.8824
 0.066667  4.5455
 0.05  3.7037
 0.04  3.3333
 0.033333  2.8571
 0.028571  2.5

1/ Enzyme Rate
 5
 2.8571
 2
 1.8182
 1.6949
 1.5873
 1.5625

The equation for lineweaver burk plot is,

This equation resembles straight line equation Y=mX + C, where, m is

the slope and c is the y-intercept.

For Enzyme:

The y-intercept in the above plot is given by 1/Vmax.

From the above plot y-intercept =0.843.

The slope in the above plot is given by Km/Vmax .

From the above plot slope =20.47

For inhibited enzyme rate 1,

The y-intercept in the above plot is given by 1/Vmax.

From the above plot y-intercept =1.514.

The slope in the above plot is given by Km/Vmax.

From the above plot slope =42.84

From the graph it is inferred the two plots does not have same slope or y-
intercept and have near about similar value of x-intercept,. Thus,
inhibition 1 represents non-competitive inhibition.

The lineweaver burk plot for the given values of inhibited enzyme rate 2
is shown below,

1/ [S] 1/ Rate 2
 0.2  7.1429
 0.1  5.5556
 0.066667  4.5455
 0.05  4
 0.04  3.7037
 0.033333  3.5714
 0.028571  3.3333

1/ Enzyme Rate
 5
 2.8571
 2
 1.8182
 1.6949
 1.5873
 1.5625

The equation for lineweaver burk plot is,

This equation resembles straight line equation Y=mX + C, where, m is
the slope and c is the y-intercept.

For inhibited enzyme rate 2,

The y-intercept in the above plot is given by 1/Vmax.

From the above plot y-intercept =2.904.

The slope in the above plot is given by Km/Vmax.

From the above plot slope = 22.21

From the graph it is inferred the two plots does not have y-intercept or x-
intercept, but have near about same values of slope. Thus, inhibition 2
represents uncompetitive inhibition.