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Methylation is one of the main ways of regulating gene expression. Studying the
methylation of chromosomal DNA is the basis for understanding gene regulation.
After processing DNA with standard bisulfite methods, unmethylated cytosine C is
deaminated to form uracil U. After PCR amplification, uracil U was replaced with
thymine T, while methylated cytosine C remained unchanged. Based on a new
generation of high-throughput sequencing platform, scientists perform low-cost,
high-efficiency, high-accuracy mapping of the genome-wide DNA methylation level.
The analysis of high-precision methylation modification patterns of specific species
will surely have a landmark significance in epigenomics research, and will be used for
the research of basic mechanisms such as cell differentiation, tissue development,
animal and plant breeding, human health, and disease.
Changes in methylation status are an important factor causing tumors, and these
changes include a decrease in the overall methylation level of the genome and an
abnormal increase in the local methylation level of CpG islands, leading to genome
instability (such as chromosome instability, activation of mobile genetic factors,
expression of proto-oncogenes) and non-expression of tumor suppressor genes. If
the active allele in the tumor suppressor gene is inactivated, the risk of cancer is
increased, for example, loss of the insulin-like growth factor-2 (IGF-2) gene imprint
leads to many tumors, such as Wilm's tumor.
In the past years, people have increasingly realized the importance of DNA
methylation research and developed a series of methods for detecting DNA.
According to the purpose of the research, these methods are divided into
methylation detection at the overall level of the genome, detection of specific site
methylation, and search for new methylation sites. According to the different
processing methods used in the research, it can be divided into methylation analysis
method based on PCR; methylation analysis method based on restriction enzyme;
methylation analysis method based on bisulfite and column layer method.
Methylation analysis methods include HPLC-UV, LC-MS, ELISA-Based Methods, LINE-
1+ Pyrosequencing, Levels of LINE-1 methylation and so on.