Haemophilus –HACEK reactions Bordetella BCYE,CAP,Mueller hinton

Catalase positive (BBF PH LH) Franciscella and TSB

Brucella Legionella  requires cysteine, thiosulfate
Bordetella except parapertussis o except F. novicida
Franciscella ODC+  ferments glocuse, maltose,
P. multocida P. multocida mannose
Haemophilus except ducreyi and H.  F. philomiragia: H2S+
aprophilus Indole + (PC)  Fluorescent stain such as
Legionella P. multocida acridine orange/faint bipolar
HACEK except A. Cardiobacterium hominis staining
actinomycetemcomitans Bordetella
Encapsulated (Bp FPH)  most are non fastidious
Oxidase positive (BBFp PH CEKL ) B, pertussis except B. pertussis
Brucella Franciscella o requires charcoal,
Bordetella except parapertussis P. multocida
blood, starch
F. philomiragia H. influenzae
 yellow green fluorescene in
P. multocida
DFA stain
Haemophilus except H. ducreyi and A. GENERALITIES
 Only B. bronchiseptica grows
segnis Haemophilus
in MAC
CEK  Most require X and V factors
Legionella  Only B. pertussis grows in
except H./A. aprophilus
Regan Lowe: Shiny silver
 No growth in SBA = requires
Urease positive (B BbBp) mercury drop looking
X and V
Brucella colonies/ mother of pearl
 Satellite growth
B. bronchiseptica (4 hrs) looking.
 Only ducreyi cant ferment o Unable to grow in
B. parapertussis (24hrs) glucose
BAP and CAP
 Only H. aprophilus is a Legionella
Motile (BBBB L) lactose fermenter
B. bronchiseptica  Requires cysteine and iron
 All inhabit the URT and oral salts for growth
B. avium cavity except H. ducreyi
B. hinzii o Slow growers:3-5
 Most are normal flora and days
B. ansorpii opportunistic pathogens
Most legionella except L. o Able to grow at
except H. influenza and
oakridgensis, nautarum, londinensis wide variety of temp
ducreyi
 Ground glass appearance in
 Encapsulated H. influenzae :
Nitrate reducer (BBb PH) BCYE with cysteine
iridescent colonies
Brucella  All are sodium hippurate
 Non- encapsulated H.
B. brochiseptica hydrolysis – except L.
influenzae: transparent bluish
P. multocida pneumophila
pigment
Haemophilus  Don’t grow in SBA
Pasteurella
 Most species produce
Obligate parasite  Glucose fermenter
fluorescence after exposure
Haemophilus  On SBA: non hemolytic,
to UV light (366nm)
unable to grow in MAC or
HACEK
Facultative Anaerobes (PH) any enteric media
 All are glucose fermenters
Pasteurella multocida  CAP/BAP: smooth gray non
except E. corrodens
Haemophilus hemolytic
(assacharolytic)
Brucella
o Doesn’t grow in
Strictly aerobes (BBF)  Sand appearance
MAC
Brucella  SBA/CAP and other media: o Bleach like odor
Bordetella smooth, raised transluscent
o Requires X factor
Franciscella colonies
 A. aprophilus: Grade school
 Only B. suis is V on H2S
paste
Assacharolytic (BBLE) production
 A. actinomycetemcomitans:
Brucella  Only B. abortus is inhibited
star shaped with 4-6 points in
Bordetella in Thionine and requires CO2
the center after 48 hrs
Legionella o Requires niacin for
E. corrodens  Rosette/dumbbell appearance
growth
in gram stain
 Replace saffranin with CF
Facultative intracellular pathogen  K. kingae: short plump rods
Franciscella
(BB FL) with square ends
 Gray-white raised, smooth
Brucella o Indole, catalase -
colonies in