ceureea 2016 — Conference Paper Paper Number 2CE16 Antioxidant activity determination and optimization of the isolated compounds from the roots of Chlorophytum borivilianum (safed musli) Khoo Chang Jian!*, Chua Bee Lin? '2School of Engineering, Taylor's University “changjian.khoo taylors.edu.my Abstract Traditional herbal medicines play a crucial role to the human body. These herbs contain many diffirent types of compounds. In this research, the plant Chlorophytum borivilianum (safed musli) and its compounds saponins were studied. Extraction and quantification of saponins from the roots of C. borivilianum (safed musli) were conducted by incorporating the ultrasonicassisted extraction (UAE) and vanillin— sulfuric acid assay respectively. The effects of extraction parameters: extraction time and extraction temperature with their respective conditions were investigated by optimization using response surface methodology (RSM). The optimal ranges of extraction parameters were identified through a single factor experimental design. Futher optimization of the optimal ranges of extraction parameters was conducted using RSM incorporated with central composite design (CCD). A second order polynomial function was fitted to the data according to the analysis obtained from ANOVA. The experimental values obtained from ANOVA were significant with 89.77% of total variation. The results show that the optimum conditions at extraction time of 46.35 ‘minutes and extraction temperature of 55.18°C provided the optimum extraction yield of saponins of 9.5768 mg/mL. Validation of model was conducted to compare the predicted and actual extraction yield of saponins. The relative error obtained between the two values was 8.371%. Therefore, it was proven that the developed second order polynomial model by RSM optimization was reliable. The antioxidant activity of the samples was studied using 2,2-diphenyl-I-picrylhydrazyl (DPPH). The results reveal that the samples possesed a very high antioxidant with scavenging activty of 88.58%. Keywords: saponins, ultrasonic-assisted extraction, optimization, central composite design, antioxidant activity. 205