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Analytical Procedure for
ACE-0003
Hydrogen Peroxide concentrate
Revision Prepared by Date Prepared
00 Will V. Castillo
Effective Date Reviewed by: Date Reviewed
Jairus J. Selim
Approved by: Date Approved
Ian Ace Pacquiao

1. Policy
In laboratory analysis, concerning procedures of hydrogen peroxide concentrate assay and as
stated in United States of Pharmacoepia a concentration (USP) a concentration range equal to 29-
32% of hydrogen peroxide should be utilized. With a suitable preservative/s not more than 0.05
%.
2. Purpose

The purpose of this SOP is to provide an analytical procedure for assay of hydrogen peroxide
concentrate with not less than 29% and not more than 32%, by weight of H 2O2. Hydrogen
peroxide in higher concentrations is used as bleach for textiles and paper, as a component for
producing foam rubber and organic chemicals. Hydrogen peroxide concentrate assay procedures
provide quantitative analysis technique to determine the quality of raw materials to ensure
efficacy.

3. Scope
4. The scope of this SOP is only limited to hydrogen peroxide concentrate used for its various
medical applications. Hydrogen peroxide Concentrate contains not less than 29.0 percent and not
more than 32.0 percent, by weight, of H 2O2. It contains not more than 0.05 percent of a suitable
preservative or preservatives.
5. Analytical Procedure
1.) Place a 100 mL volumetric flask on an analytical balance. Close the balance door.
Tare the flask by briefly pressing the control bar. The readout will display zero with
the flask sitting on the pan. This will allow the mass of your sample to be read
directly.
2.) Fill the tared volumetric flask with the prepared hydrogen peroxide concentrate up to
1 mL mark. Record the weight of the sample.
 3.) After the sample has been weighed. Remove the flask and proceed to your respective
experimentation area.
4.) To dilute the solution – Using a graduated cylinder, accurately measure 19 mL of
distilled water and pour it into the volumetric flask containing H 2O2 concentrate.
5.) Mix the solution using a stirring rod. Stir the solution clockwise 5 times and counter-
clockwise 10 times.
6.) Measure 20 mL of the prepared 2N sulphuric acid using a graduated cylinder and
pour it to the flask containing H2O2 solution. Set aside.
7.) Using iron stand, burette clamp and burette, prepare a titration setup. Then, make
sure that stopcock is close.
8.) Fill the burette with 0.1 N potassium permanganate up to the “0” mark.
9.) Place the volumetric flask containing H2O2 solution on the base of the iron stand,
directly below the burette to prepare for titration.
10.) Slowly turn the stopcock valve to allow the movement of titrant (0.1N potassium
permanganate) at definite rate, from the burette into the volumetric flask and perform
the titration.
11.)Continue titration until the solution appears to be clear or endpoint is reached.
Document ID: ACE-0003 Title: Assay of Sodium Nitrite Page #: 1/2

Prepared by Reviewed by
Will Castillo Jairus Selim

Approved by:
Ian Ace Pacquiao
Document ID: ACE-0004 Title: Assay of Sodium Nitrite Page #: 2/2