[I] = 0 Mm

[G] (micro moles de G/ ml de sln)

tiempo (min9) [G6p] 10mM [G6p] 20mM [G6p] 40mM [G6p] 80mM tiempo (min9)
0 0 0 0 0 0
1.5 89.95 140.04 193.92 240.07 1.5
3 141.13 219.73 304.25 376.67 3
4.5 215.79 335.96 465.21 575.93 4.5
6 365.35 568.81 787.64 975.1 6

[I] = 40 Mm
[G] (micro moles de G/ ml de sln)
tiempo (min9) [G6p] 10mM [G6p] 20mM [G6p] 40mM [G6p] 80mM tiempo (min9)
0 0 0 0 0 0
1.5 53.4 86.33 124.76 160.52 1.5
3 83.79 135.44 195.75 251.85 3
4.5 128.11 201.09 299.3 385.09 4.5
6 216.9 350.63 506.75 651.99 6
[G6p] mM V1 V2 V3 V4
0 20 40 80 1/v vs 1/s
10 57.103 42.601 33.901 24.103 0.045
20 88.903 67.803 54.401 39.601 0.04 f(x) = 0.324937694511723 x + 0.008998
40 123.1 96.403 79.203 58.401 0.035 R² = 0.999999812806858
80 152.4 122.1 101.9 76.602 0.03
f(x) = 0.225052305210325 x + 0.007030

Axis Title
0.025 R² = 0.999942426590787
0.02 f(x) = 0.174677104089243 x + 0.006008
R² = 0.999999599822759
1/[G6p] mM 1/v1 1/v2 1/v3 1/v4 0.015 f(x) = 0.125156904212647 x + 0.004994
R² = 0.999999595772528
0.1 0.01751221 0.02347363 0.02949765 0.04148861 0.01
0.05 0.01124821 0.01474861 0.01838202 0.02525189 0.005
0.025 0.00812348 0.01037312 0.01262578 0.01712299 0
0 0.02 0.04 0.06 0.08 0.1
0.0125 0.00656168 0.00819001 0.00981354 0.01305449
Axis Title

A B Vmax ap Km ap Tipo de inhibición

1/v1 0.005 0.1252 200 25.04
1/v2 0.006 0.1747 166.666667 29.1166667
1/(𝑉𝑚𝑎𝑥 𝑎𝑝)= ([𝐼])/(𝑉𝑚𝑎𝑥∗𝐾1 𝑖𝑛 )+ 1/(𝑉𝑚𝑎𝑥
1/v3 0.007 0.2251 142.857143 32.1571429
1/v4 0.009 0.3249 111.111111 36.1

A
[I] 1/Vmax ap B
0 0.005 Vmax
20 0.006 K1in
40 0.007
80 0.009 (𝐾𝑚 𝑎𝑝)/(𝑉𝑚𝑎𝑥 𝑎𝑝)= 𝐾𝑚/𝑉𝑚𝑎𝑥+ (𝐾𝑚 [𝐼])/(𝑉𝑚𝑎𝑥 ∗𝐾

[I] (Km ap)/(Vmax ap)

0 0.1252 A
20 0.1747 B
40 0.2251 Km
80 0.3249 K2 in
[I] = 20 Mm
[G] (micro moles de G/ ml de sln) [I] = 0 Mm
[G6p] 10mM [G6p] 20mM [G6p] 40mM [G6p] 80mM
0 0 0 0 1200
67.11 106.8 151.86 192.34
1000
105.29 167.57 238.26 301.78
160.99 256.22 364.31 461.43 800 f(x) = 152.404 x − 23.6580000000001
R² = 0.963892544116189
Axis Title

272.57 433.81 616.8 781.23 600

f(x) = 123.104666666667 x − 19.11
R² = 0.963890759748434
f(x) = 88.9026666666667 x − 13.8
[I] = 80 Mm 400 R² = 0.963894587191838
[G] (micro moles de G/ ml de sln) f(x) = 57.1026666666667 x − 8.86400000000007
200 R² = 0.963892711592849
[G6p] 10mM [G6p] 20mM [G6p] 40mM [G6p] 80mM
0 0 0 0 0
0 1 2 3 4 5 6 7
37.93 62.38 92 120.67
Axis Title
59.57 97.87 144.34 189.32
91.08 149.65 220.7 289.48
154.2 253.37 373.66 490.11

[I] = 40 Mm
700

600
 [I] = 40 Mm
700

600
1/v vs 1/s f(x) = 101.903333333333 x − 15.8200000000001
R² = 0.963891024733427
500
f(x) = 79.2026666666667 x − 12.2960000000001

Axis Title
400
R² = 0.963889088019288
f(x) = 0.324937694511723 x + 0.008998041285504 1/v1
R² = 0.999999812806858 300
Linear f(x) = 54.4013333333333 x − 8.50600000000005
(1/v1) R² = 0.957863811021873
200
f(x) = 0.225052305210325 x + 0.007030422452937 1/v2 f(x) = 33.9006666666667 x − 5.262
R² = 0.999942426590787 100 R² = 0.963898101111674
f(x) = 0.174677104089243 x + 0.006008352416214 Linear
R² = 0.999999599822759 (1/v2)
0
f(x) = 0.125156904212647 x + 0.004994666553505 1/v3 0 1 2 3 4 5 6 7
R² = 0.999999595772528 Linear
(1/v3) Axis Title
1/v4
Linear
0.02 0.04 0.06 0.08 0.1 0.12 (1/v4)
Axis Title

Mixta tipo I 1/Vmax ap

0.01
0.009
[𝐼])/(𝑉𝑚𝑎𝑥∗𝐾1 𝑖𝑛 )+ 1/(𝑉𝑚𝑎𝑥 ) f(x) = 5E-05 x + 0.005
0.008 R² = 1
0.007
0.006
Axis Title

0.005 1/Vmax ap
0.005 Linear (1/Vmax ap)
0.004
5.00E-05
0.003
200 0.002
1.00E+02 0.001
0
𝐾𝑚/𝑉𝑚𝑎𝑥+ (𝐾𝑚 [𝐼])/(𝑉𝑚𝑎𝑥 ∗𝐾2 𝑖𝑛) 0 10 20 30 40 50 60 70 80 90
Axis Title

0.125
0.0025
25 (Km ap)/(Vmax ap)
50 0.35

0.3 f(x) = 0.002498142857143 x + 0.12504

R² = 0.999993799809688
0.25
Axis Title

0.2
(Km ap)/(Vmax ap)
0.15 Linear ((Km ap)/(Vmax ap))

0.1

0.05

0
0 10 20 30 40 50 60 70 80 90
Axis Title
 Linear ((Km ap)/(Vmax ap))

Axis
0.15

0.1

0.05

0
0 10 20 30 40 50 60 70 80 90
Axis Title
[I] = 0 Mm [I] = 20 Mm
900
800
[G6p] 10mM 700 [G6p] 10mM
f(x) = 122.103333333333 x − 18.954 Linear ([G6p] 10mM)
23.6580000000001 Linear ([G6p] 10mM) 600 R² = 0.963895163958697
116189 [G6p] 20mM [G6p] 20mM
Axis Title

500 f(x) = 96.4033333333333 x − 14.964

666667 x − 19.11 Linear ([G6p] 20mM) R² = 0.963893528188743 Linear ([G6p] 20mM)
748434 [G6p] 40mM 400 [G6p] 40mM
666667 x − 13.8 f(x) = 67.8026666666667 x − 10.528 Linear ([G6p] 40mM)
Linear ([G6p] 40mM) 300 R² = 0.963888183776445
191838 [G6p] 80mM
[G6p] 80mM 200 f(x) = 42.6013333333333 x − 6.612
666667 x − 8.86400000000007 Linear ([G6p] 80mM)
592849 Linear ([G6p] 80mM) R² = 0.96389043086954
100
0
4 5 6 7 0 1 2 3 4 5 6 7

itle Axis Title

[I] = 80 Mm
[I] = 40 Mm 600

500
[G6p] 10mM
f(x) = 76.602 x − 11.8900000000001 Linear ([G6p] 10mM)
 [I] = 80 Mm
[I] = 40 Mm 600

500 [G6p] 10mM

f(x) = 76.602 x − 11.8900000000001 Linear ([G6p] 10mM)
3333333 x − 15.8200000000001 [G6p] 10mM 400
4733427 R² = 0.963891505505027 [G6p] 20mM

Axis Title
Linear ([G6p] 10mM)
300 f(x) = 58.4013333333333 x − 9.06400000000004 Linear ([G6p] 20mM)
6666667 x − 12.2960000000001 [G6p] 20mM
R² = 0.963892615255908 [G6p] 40mM
8019288 Linear ([G6p] 20mM)
200 f(x) = 39.6006666666667 x − 6.14800000000002 Linear ([G6p] 40mM)
[G6p] 40mM
3333333 x − 8.50600000000005 R² = 0.963889794003431 [G6p] 80mM
1021873 Linear ([G6p] 40mM)
100 f(x) = 24.1033333333333 x − 3.75400000000003 Linear ([G6p] 80mM)
[G6p] 80mM R² = 0.963939557531529
6666667 x − 5.262
1111674 Linear ([G6p] 80mM) 0
0 1 2 3 4 5 6 7

4 5 6 7 Axis Title
Title
[G6p] 10mM
Linear ([G6p] 10mM)
[G6p] 20mM
Linear ([G6p] 20mM)
[G6p] 40mM
Linear ([G6p] 40mM)
[G6p] 80mM
Linear ([G6p] 80mM)

[G6p] 10mM
Linear ([G6p] 10mM)
 [G6p] 10mM
Linear ([G6p] 10mM)
[G6p] 20mM
0004 Linear ([G6p] 20mM)
[G6p] 40mM
0002 Linear ([G6p] 40mM)
[G6p] 80mM
0003 Linear ([G6p] 80mM)
Abs [g/L]
0.014 0 [g/L]
0.043 0.1 7
-0.832 0.2 6
0.162 0.5
f(x)5 = 1.50166350203571 x + 0.947134264828679
-0.927 1 R² = 0.876865693653763
Axis Title

0.678 2 4

1.426 3 3
2.305 4 2
2.432 5
1
2.909 6
0
-1.5 -1 -0.5 0 0.5 1 1.5 2 2.5 3 3.5
Axis Title
 Muestra 0,25ml 8 ml de sln
[g/L] Agua 7.75

Factor de dilucion 1:32

Abs sin dilucion 3.741
66350203571 x + 0.947134264828679
65693653763 Abs con dilucion 1.788
[g/L]
Linear ([g/L]) [Azúcar reductor] 116.2284

En este tiempo la concentracion de azucar reductor es 116,228

1 1.5 2 2.5 3 3.5

xis Title
azucar reductor es 116,228
 ln⁡〖𝑉𝑚𝑎𝑥 𝑎𝑝 =𝐿𝑛

ln⁡〖𝐾𝑚 𝑎𝑝 =𝐿𝑛 𝐾𝑚 𝑎𝑝(

 T= 15°C T= 25°C
[AA] Mm 0 10 20 30 0
Vmax ap 180 179.1 179.1 182.1 237.3
Km apa 3.5 5.21 6.99 8.88 3.21

Chart Title
Tipo de inhibición Competitiva 10
9
𝐾𝑚 𝑎𝑝 = Km+ (𝐾𝑚 8 f(x) = 0.1792 x + 3.457
R² = 0.999490802701609
[𝐼])/(𝐾𝑖 𝑐) 7
f(x) = 0.1289 x + 3.204
T= 15 T= 25 6 R² = 0.999967500714683

Axis Title
5
Km 3.457 3.204
4
Ki c 19.29129464 24.8564779
3
Vmax 180.075 237.475 2
1
0
0 5 10 15 20 25 30
Para hallar la Ea
Axis Title

ln⁡〖𝑉𝑚𝑎𝑥 𝑎𝑝 =𝐿𝑛 𝑉𝑚𝑎𝑥 𝑎𝑝(0)−𝐸𝑎/𝑅𝑇 〗

1/T Ln Vmax ap Ln Vmax ap vs 1/t

0.00347222222222 5.193373431 6
0.00335570469799 5.470062355
5 f(x) = − 2374.65502134462 x + 13.4387033659998
R² = 1
Ea 4701.906
4
Ln Vmax ap (0) 13.439
3
Para hallar el dH
2

ln⁡〖𝐾𝑚 𝑎𝑝 =𝐿𝑛 𝐾𝑚 𝑎𝑝(0)+𝑑𝐻/𝑅𝑇 〗 1

0
1/T Ln Km ap 0.00334 0.00336 0.00338 0.0034 0.00342 0.00344 0.00346 0.
0.00347222222222 1.240401161
0.00335570469799 1.164400029
 T= 25°C
10 20 30
237.3 237.7 237.6
4.48 5.79 7.07

Chart Title

= 0.1792 x + 3.457
= 0.999490802701609
= 0.1289 x + 3.204 T= 15°C
= 0.999967500714683
Linear (T= 15°C)
T= 25°C
Linear (T= 25°C)

5 10 15 20 25 30 35
Axis Title

ap vs 1/t

+ 13.4387033659998

0.00342 0.00344 0.00346 0.00348