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Fluorescence Concentration Report

This document summarizes the results of a fluorescence calibration and sample analysis. It includes information on instrument parameters, calibration standards from 0.05 to 0.25 mg/L with a linear calibration curve, and fluorescence readings for 5 samples. Sample 1 was over the concentration range and sample 2 had a concentration of 0.358 mg/L based on the calibration.

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IsabellaNoreña
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69 views2 pages

Fluorescence Concentration Report

This document summarizes the results of a fluorescence calibration and sample analysis. It includes information on instrument parameters, calibration standards from 0.05 to 0.25 mg/L with a linear calibration curve, and fluorescence readings for 5 samples. Sample 1 was over the concentration range and sample 2 had a concentration of 0.358 mg/L based on the calibration.

Uploaded by

IsabellaNoreña
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

2.

1.5
Intensity (a.u.)

1.0

0.5

0.0

-0.5
0.00 0.05 0.10 0.15 0.20 0.25
Concentration (mg/L)

Zero: Ex 350.00 ; Em 450.00 ( 0.896 )


Zero: Ex 350.00 ; Em 450.00 ( 0.987 )

Concentration Report
Report time: 8/28/2019 [Link] PM
Software version: 1.1(132)
Operator:

Instrument Parameters
Instrument Cary Eclipse
Instrument Serial Number FL1108M003
Data mode Fluorescence
Excitation wavelength (nm) 350.00
Emission wavelength (nm) 450.00
Ex. Slit (nm) 5
Em. Slit (nm) 10
Excitation filter Auto
Emission filter Open
Ave time (sec) 0.10000
PMT voltage (V) Medium
Standard replicates 3
Standard averaging OFF
Sample replicates 1
Sample averaging OFF
Weight and volume corrections OFF
Fit type Linear
Min R² 0.95000
Concentration units mg/L
Comments:

Calibration
Collection time 8/28/2019 [Link] PM

Standard Concentration F Mean SD %RSD Readings


mg/L
________________________________________________________________________
Std 1 -0.112
-0.001
0.050 -0.013 0.0934 >100 0.073

Std 2 0.642
0.537
0.100 0.573 0.0604 10.5 0.539

Std 3 0.732
0.979
0.150 0.826 0.1335 16.1 0.767

Std 4 1.213
1.112
0.200 1.241 0.1452 11.7 1.398

Std 5 1.592
1.639
0.250 1.612 0.0241 1.49 1.604

Calibration eqn Int = 7.83542*Conc -0.32774


Correlation coefficient 0.98684
Calibration time 8/28/2019 [Link] PM

Analysis
Collection time 8/28/2019 [Link] PM

Sample Concentration F Readings


mg/L
_________________________________________________
Sample 1 101.204 O 792.646
Sample 2 0.358 O 2.477
Sample 3 0.154 0.881
Sample 4 0.110 0.536
Sample 5 0.120 0.610
Read sequence cancelled

Results Flags Legend


U = Uncalibrated O = Over-range concentration
N = Not used in calibration R = Repeat reading
@ = Over-range signal

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