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Original article

Evaluation of the chemical characteristics and rheological


behavior of pitaya (Hylocereus undatus) peel
Fernanda Robert DE MELLO1*, Cláudia BERNARDO3, Carolinne Odebrecht DIAS3, Luana Carolina BOSMULER ZÜGE1,
Joana Léa MEIRA SILVEIRA4, Edna Regina AMANTE3, Lys Mary BILESKI CANDIDO2

1 Technol. Sector-Fed. Univ. Evaluation of the chemical characteristics and rheological behavior of pitaya
Paraná, PO Box 19011, (Hylocereus undatus) peel.
Curitiba PR, Brazil,
nandarobert@hotmail.com, Abstract – Introduction. Pitaya peel has been applied as a functional ingredient for food
nandarobert@gmail.com due to the presence of betacyanins. However its polysaccharides can also contribute as a tex-
2 Dep. Chem. Biol., ture agent for food. The aim of this work was to evaluate the chemical characteristics and
Fed. Technol. Univ. Paraná, rheological behavior of the pitaya peel. Materials and methods. The samples were analyzed
Curitiba PR, Brazil with regard to moisture and mineral content, protein, lipids, sugar, fiber, vitamin C, titratable
3
Dep. Food Sci. Technol., acidity, soluble solids content and pH. Rheological measurements were performed on rheo-
Cent. Agric. Sci., meter through flow curve, stress sweep, frequency sweep and the variation of temperature.
Fed. Univ. Santa Catarina, Results and discussion. The results showed that pitaya peel is rich in insoluble fibers and
Florianópolis SC, Brazil exhibits non-Newtonian behavior, characteristic of a strong gel with a predominance of solid
4 Dep. Biochem. Mol. Biol., character. Furthermore, samples showed thermal resistance at the conditions of frequency,
Fed. Univ. Paraná, tension and temperature analyzed. Conclusion. Considering our results, in addition to use as
PO Box 19031, Curitiba PR, a natural colorant in food, pitaya peel can also contribute to the nutritional value and texture
Brazil of the products.

Brazil / Hylocereus undatus / fruits / byproducts / peel / chemical composition /


fibers / rheological properties

Évaluation des caractéristiques chimiques et du comportement rhéologique


de la pelure du pitaya (Hylocereus undatus).
Résumé – Introduction. La pelure du pitaya est utilisée comme ingrédient alimentaire en
raison de la présence de bétacyanines. Cependant ses polysaccharides peuvent également
contribuer à la nutrition en tant qu'agent de texture. Le but de notre travail a été d'évaluer les
caractéristiques chimiques et le comportement rhéologique de la pelure de pitaya. Matériel
et méthodes. Des échantillons de pelure de pitaya ont été analysés quant à leur teneur en
humidité et en sels minéraux, protéines, lipides, sucres, fibres, vitamine C, acidité titrable,
* Correspondence and reprints
matières solides solubles, ainsi que leur pH. Des mesures rhéologiques ont été effectuées
avec un rhéomètre en étudiant la courbe d'écoulement, le balayage du fluage, le balayage de
Received January 4, 2014 fréquence et la variation de température. Résultats et discussion. Les résultats ont montré
Accepted March 4, 2014 que la pelure de pitaya est riche en fibres insolubles et présente un comportement non-new-
tonien, caractéristique d'un gel avec prédominance d’un caractère solide. En outre, les échan-
tillons ont montré une résistance thermique aux conditions de fréquence, tension et
Fruits, 2014, vol. 69, p. 381–390 température de l’analyse. Conclusion. Compte tenu de ces résultats, en plus d'être appliquée
© 2014 Cirad/EDP Sciences en tant que colorant naturel dans l'alimentation, la pelure de pitaya pourrait également contri-
All rights reserved buer à la valeur nutritionnelle et à la texture des produits.
DOI: 10.1051/fruits/2014028
www.fruits-journal.org
Brésil / Hylocereus undatus / fruits / sous-produit / pelure / composition
RESUMEN ESPAÑOL, p. 390 chimique / fibre / propriété rhéologique

Article published by EDP Sciences

Fruits, vol. 69 (5) 381


F.R. de Mello et al.

1. Introduction According to Jamilah et al., the total fiber


content of pitaya is very high (69.3%), of
Pitaya is a tropical fruit and its name, of Anti- which 56.50% corresponds to insoluble fiber
llean origin, means scaly fruit. This fruit (IF) and 14.82% to soluble fiber (SF) [14].
belongs to the Cactaceae family from which The main polysaccharides present are
various species of epiphytic cacti of the pectins, hemicelluloses and cellulose, lignin
genus Hylocereus and Selenicereus, native and starch. Pectin fractions were mainly
to Mexico and Central and South America, composed of arabinose, galactose and
are also cultivated in the countries of South- rhamnose, while the hemicellulose fraction
east Asia [1, 2]. mainly consisted of glucose and xylose [18].
Hylocereus undatus is the most cultivated Knowledge of the rheological properties
and consumed species of pitaya. The fruits of raw materials used in the food industry
of this species present market demand, due is essential for the development of new food
to its very attractive sensory characteristics. products, because different materials will
respond differently to external forces which
Despite considerable research on its
they are subjected to [19].
nutritional importance, medicinal and food
value, the pitaya is still a culture not widely The rheological behavior of fluid food
used and exploited [3]. The fruit has a high such as fruit juices and pulps is a factor of
nutritional value, and is rich in calcium, great importance in the design of equip-
phosphorus, potassium and vitamins. Fur- ment in the food industry; in addition, it is
thermore, it can also be considered as a one of the factors that helps to evaluate the
source of carbohydrates and fibers [4, 5]. quality of the product. The rheological
behavior of these materials, which are
Studies conducted with the pitaya em-
mainly comprised of water and the various
phasized its functional properties helping to
solids, such as soluble and insoluble fibers,
reduce the risk of chronic diseases and its
results from the interaction between these
potential to contribute to physical and men-
elements contributing alone, or potentiated
tal wellness [6–8]. Pulp showed antioxidants
[9, 10], and oligosaccharides with prebiotic when combined [19].
properties [8]. Pitaya peel presented high Therefore, pitaya peel presents high
quantities of antioxidants [11]. Moreover, its potential as a functional ingredient for food
seeds are rich in essential fatty acids and which can improve its nutritional value and
phytosterols [12, 13]. texture. The aim of our study was to evalu-
The pitaya peel represents up to one third ate the chemical composition and rheolog-
of the fruit weight; it is considered a residue ical behavior of the pitaya peel in order to
of the pitaya processing industry [14, 15]. provide information which could be helpful
The waste of fruit and vegetable processing for its application in food products.
is traditionally considered an environmental
problem, however it has been increasingly
used for the extraction of compounds such
as pigments [16]. Pitaya peels may be sub-
2. Materials and methods
jected to simple and economical purification
methods to provide extracts and sub-frac- 2.1. Materials
tions for the formulation of healthcare prod-
ucts and food applications [15]. Samples of pitaya (Hylocereus undatus)
Aside from being rich in antioxidants [11] (figure 1) were harvested at Embrapa,
and phenolic compounds [17], pitaya peel Brasília, Brazil, in 2011 and 2012, January.
presents high concentration of fibers and The fruits (30 units) were packaged and
polysaccharides which are responsible of its transported to the Laboratory of Fruits and
texture and rheological behavior, and can Vegetables of the Federal University of Santa
be useful for the food industry as a texture Catarina, where they were stored at a tem-
agent. perature of 6 °C.

382 Fruits, vol. 69 (5)


Characteristics and rheological behavior of pitaya

The reagents used were all of analytical


grade. The enzymes α-amylase, protease,
amyloglucosidase were obtained from
Megazyme (Ireland). The other reagents
(sodium hydroxide, ether, sulphuric acid,
anhydrous sodium sulfate, copper sulfate,
boric acid, ethanol, hydrochloric acid,
oxalic acid, ascorbic acid, sodium dichlo-
rophenolindophenol (DCFI), zinc acetate,
copper sulfate, sodium potassium tartrate,
acetone, zinc acetate, potassium ferrocya-
nide) were obtained from Vetec (Rio de
Janeiro, Brazil). The water used was filtered
through a deionization system (Milli-Q
deionizer, Millipore, Bedford, USA).

2.2. Methods

2.2.1. Sample preparation


The fruits were washed and manually sep- Figure 1.
arated in exocarp (dirt, scales and cladode Pitaya fruit (Hylocereus undatus).
fragments), mesocarp (peel and endocarp)
and pulp with seeds (figure 2).
The fresh mesocarp was used for chem-
ical analysis. Part of the pitaya peel was
dehydrated in an oven (DLS04, DeLeo,
Brazil) at 45 °C for about 12 h. Samples
were ground in a grinder mill (Q298 A21,
Quimis, Brazil) in order to obtain a homo-
geneous mixture which was passed through
standard sieves of 100 mesh (150 µm) for
analysis.
For the rheological analysis, dehydrated
pitaya peels were rehydrated with 90%
water and homogenized (L4RT, Silverson
Machines Ltd., U.K.) at 9000 rpm for 3 min.

2.2.2. Chemical characterization


The chemical analysis was performed with
the mesocarp of three fruits of each harvest
(2011 and 2012).
of soluble solids (°Brix) were obtained on Figure 2.
Analyses of fibers (soluble and insoluble) a digital refractometer (LLI 58,318, Mettler Preparation of samples for
and sugars (reducing and non-reducing) Toledo, Switzerland). The titratable acidity evaluating chemical
were performed in duplicate. All other ana- was measured by titration with 0.1 mol⋅L–1 characteristics and rheological
lyzes were performed in triplicate according NaOH and acidity expressed as percent behavior of pitaya (Hylocereus
to the standards recommended by the Asso- undatus) peel.
standard solution.
A color figure is available at
ciation of Official Analytical Chemists The moisture content was obtained by www.fruits-journal.org.
(AOAC) [20]. oven drying (DLS04, DeLeo, Brazil) at
The pH readings were taken with a digital 105 °C until constant weight. The ash was
pH meter (Q-400A, Quimis, Brazil) cali- determined by incinerating the sample at
brated with buffers 4.0 and 7.0. The values 550 °C in a muffle (Q-318M24, Quimis,

Fruits, vol. 69 (5) 383


F.R. de Mello et al.

Brazil) to constant weight. The protein was The frequency sweep was then conducted
analyzed by the Kjeldahl method, based on applying the preselected strain, increasing
nitrogen determination by digestion of the oscillatory frequency over time from (0.1
organic matter. Lipids were extracted with to 100) Hz at 25 °C.
ethylic ether by the Soxhlet method.
The rheological behavior of the sample was
The soluble and insoluble fibers were also evaluated with variation of temperature,
determined through enzymatic-gravimetric using a heating program of 3 °C⋅min–1,
methods. The non-reducing and reducing increasing the temperature from (5 to
sugars were determined by acid hydrolysis 95) °C, at a frequency of 1 Hz. In order to
and the results expressed in reducing car- prevent solvent evaporation, a layer of min-
bohydrates into glucose and non-reducing eral oil was applied around the rheometer
carbohydrates as sucrose. The vitamin C plate. This temperature range was chosen
content was quantified by the Tillmans due to the storage temperature in the refrig-
method based on reducing sodium erator, approximately 5 °C, and the pasteur-
2,6-dichlorophenolindophenol (DCFI) and ization temperature of fruit juices, approxi-
expressed as mg of ascorbic acid.100 g–1 of mately 95 °C.
the product.

2.2.3. Rheological analysis


Rheological measurements were performed 3. Results and discussion
on rheometer (Mars II, Thermo Electron
GmbH, Germany) coupled to a thermostatic 3.1. Chemical characterization
bath (K15, Haake, Germany), a thermo
circulator water (DC5, Haake, Germany),
According to our results, pitaya peel has a
and a thermal controller (Peltier, Haake,
high concentration of water (2011 harvest =
Germany), using sensor plate-plate.
90.23%; 2012 harvest = 84.86%) (table I).
The flow curves were obtained applying Jamilah et al. reported that pitaya peel from
shear rates ranging from 0.1 to 300 s-1 at Hylocereus polyrhizus species presents
25 °C, with 100 data acquisition points, in 92.65% moisture content [14].
triplicate. The results obtained from the flow
curve were fitted using the power The samples showed low concentra-
law (Ostwald-de-Waele): τ = k (γ)n, and the tions of mineral content (2011 harvest,
Herschel-Bulkley model: τ = τ0 + k (γ)n [21, 0.17 g⋅ 100 g–1; 2012 harvest, 0.22 g⋅100 g–1),
22], where τ = shear stress (Pa), τ0 = yield proteins (2011 harvest, 0.64 g⋅100 g–1; 2012
stress (Pa), γ = shear rate (s–1), K = consist- harvest, 0.66 g⋅100 g–1) and lipids (2011
ency index (Pa⋅s–1), n = flow behavior index harvest, 0.02 g⋅100 g–1; 2012 harvest,
(dimensionless). 0.07 g⋅100 g–1) (table I). The protein con-
tent of pitaya varies considerably from
The parameters k and n are widely used (0.3 to 1.5) g⋅100 g–1 [24, 25]. This differ-
in the characterization of fluids. For a New- ence can be attributed to the methodolo-
tonian fluid, n = 1. When n is different to gies applied or to the possible interference
1, a non-Newtonian fluid is characterized. In of other nitrogen compounds such as
this case, if n is less than 1, the fluid shows
betalains.
pseudoplastic behavior, and if n is more
than 1, the fluid shows dilatant behavior. Pitaya peel from the 2011 harvest pre-
The k values are related to the resistance to sented less reducing sugars (0.70 g⋅100 g–1)
fluid flow [23]. than that of the 2012 harvest (2.62 g⋅100 g–1)
(table I). Wichienchot et al. reported that
A stress sweep was performed in the
the main reducing sugars of pitaya are glu-
range of (0.1 to 10) Pa at frequencies of
cose and fructose [8].
(1 and 10) Hz at 25 °C, in order to verify the
linear viscoelastic range and to select the The non-reducing sugar levels were
strain that would be used to perform the lower than 0.5 g.100 g–1 in all samples ana-
frequency sweep and temperature ramps. lyzed. This result is in agreement with

384 Fruits, vol. 69 (5)


Table I.
Chemical characteristics of pitaya (Hylocereus undatus) peel from samplings done on the 2011 and 2012 harvests. Data are the average from
three repetitions.
Year of Moisture Mineral Protein Lipids Reducing Non– Soluble Insoluble Vitamin C Titratable acidity Soluble pH
harvest content content sugars reducing fiber fiber (mg.100 g–1) (mg malic solids
sugars acid.100 g–1) (°Brix)
(%)
2011 90.23 ± 0.250 0.17 ± 0.005 0.64 ± 0.002 0.02 ± 0.008 0.70 ± 0.000 < 0.5 2.14 ± 0.00 2.90 ± 0.00 7.62 ± 2.21 0.22 ± 0.06 7.15 ± 0.05 5.48 ± 0.06
2012 84.86 ± 0.650 0.22 ± 0.180 0.66 ± 0.006 0.07 ± 0.030 2.62 ± 0.020 < 0.5 1.30 ± 0.99 5.70 ± 0.71 7.04 ± 0.88 0.25 ± 0.04 12.77 ± 1.01 4.83 ± 0.03
Characteristics and rheological behavior of pitaya

Fruits, vol. 69 (5)


385
F.R. de Mello et al.

Stintzing et al. who reported that the pitaya blood sugar in people with type II diabetes
juice is devoid of sucrose [25]. [5]. Moreover, pitaya peel presents mucilage
Physicochemical properties of dietary that can have a positive influence on cho-
fiber include solubility, viscosity, water and lesterol metabolism [30].
oil holding capacity, stabilizing, gel-forming Hadi administered pitaya extracts to
and fermentation. Compared with insoluble hyperglycemic rats and observed that, after
dietary fiber, in food processing, the soluble seven weeks of supplementation, the mice
fraction demonstrates greater capacity to showed reduced levels of glucose in the
provide viscosity, ability to form gels and/ blood, triglycerides and LDL cholesterol,
or act as emulsifiers. Insoluble fiber usually and increased levels of HDL cholesterol and
has high water-holding capacity. antioxidants in the plasma [31].
Insoluble fiber (IF) was present in greater Pitaya is a fruit with low levels of
quantity than soluble fiber (SF) in all sam- vitamin C [4]. Pitaya peel presented (7.62
ples. The [IF/SF] ratio was [2.90/2.14] in the and 7.04) mg⋅100 g–1 of vitamin C in
2011 harvest and [5.70/1.30] in the 2012 har- the 2011 and 2012 harvests, respectively
vest. Jamilah et al. reported that pitaya peel (table I). Although low, these levels of
presents a relation of [IF/SF] of [3.8/1.0] [14]. vitamin C are in the same range of other
According to Horn, the recommended ratio fruits, such as apple (6.0 mg⋅100 g–1) and
of [IF/SF] in food is [3/1] [26]. Therefore, the plum (3.0 mg⋅100 g–1) [32]. Choo and Yong
pitaya peel from the 2012 crop presented a reported that the pitaya pulp with seeds pre-
good [IF/SF] ratio, indicating the presence of sented 31.1 mg⋅100 g–1 of vitamin C while
dietary fiber with good physiological effect. pitaya fruit (pulp + seeds + peel) presented
11.6 mg⋅100 g–1 of vitamin C [33].
Different values were obtained by
Zhuang et al. that determined the contents Pitaya peel has low acidity: (0.22 and
of total fiber (TF), soluble fiber (SF), insol- 0.25) mg of malic acid⋅100 g–1 of sample
uble fiber (IF) of fiber rich powders (FRP) for the 2011 and 2012 harvests, respectively
from pitaya peels with different particle (table I). The acidity and soluble solids
size [27]. Dried peels were milled and content of the fruits are considered as
meshed through the 80 (FRP 80), 140 (FRP important quality criteria for the flavor clas-
140) and 250 (FRP 250) meshes. Results sification. The total acidity decreases with
showed that FRP 140 had the highest total fruit ripening due to the respiration process
fiber (79.37%) and soluble fiber (33.07%) and use of organic acids as substrates in
with a balanced ratio of [SF/IF] [1.00/1.32]. metabolic reactions [34].
The [SF/IF] ratios of FRP 80, and FRP 250 Pitaya peel presented (7.15 and
were [1.00/1.99], and [1.00/1.41], respec- 12.77) °Brix for the 2011 and 2012 harvests,
tively. The particle size of milled peels respectively (table I). Chitarra and Chitarra
affected the fiber compositions. reported that the fruits in general have a
From the physiological viewpoint, die- mean soluble solid between (8.0 and
tary fiber generally has properties such as 14.0) °Brix [35]. Junqueira et al. found that
decreased intestinal transit time and values of soluble solids of pitaya pulp
increased stool bulk; it is fermentable by ranged between 13.0–15.0 °Brix [2].
colonic micro flora; it reduces total blood The pH values for pitaya peel were high
and/or LDL cholesterol levels; it reduces (2011 harvest: 5.48; 2012 harvest: 4.83).
postprandial blood glucose and/or insulin These results are in agreement with Stintz-
levels, contributing to reduction in the risk ing et al. [25] and Fernandes et al. [36] who
of chronic disorders, e.g., coronary heart reported that pitaya pulp has a pH of 4.82
disease, diabetes, obesity and some forms and 4.6, respectively.
of cancer [28, 29].
The soluble fibers present in pitaya peel 3.2. Rheological analysis
can help in the digestive process, neutralize
toxic substances such as heavy metals and Our results regarding ground pitaya peel
may be associated with the regulation of in water showed that the flow curve

386 Fruits, vol. 69 (5)


Characteristics and rheological behavior of pitaya

consistency index (k) = 132.14 ± 6.34, flow


behavior index (n) = 0.20 ± 0.01 and
Shear stress (Pa)

R2 = 0.99. These values confirmed that the


flow curves are typical of pseudoplastic flu-
ids, because the value of (n) is less than 1
(0.20) in both models, with a good correla-
tion coefficient (R2 = 0.99). The Herschel-
Bulkley model shows that the yield stress is
not significant, since its value is lower than
the standard deviation.
Figure 3. Krokida et al. performed a study compar-
Flow curve of pitaya peel sample representing the ing various rheological data available in
shear rate dependence of the shear stress,
the literature for fruit pulps, including
recorded at 25 °C.
guava, raspberry, pineapple, apricot, apple,
mango, tamarind, and blackcurrant and
found that all these pulps fruit exhibit pseu-
doplastic behavior [37].
The effect of the ground pitaya peel in
water sample on the mechanical response
was investigated by dynamic oscillatory
tests. These assessments are sensitive to
changes in chemical composition and in the
physical structure of the samples due to the
characteristics of non-destructive measuring
method. Stress sweep experiments (1.0 Hz
and 10.0 Hz) were carried out to ensure that
Figure 4. the frequency sweep measurements were
Effect of frequency on storage modules (G': performed within the linear viscoelastic
elastic modulus) and loss (G'': viscous modulus) region. Considering the mechanical spectra
of pitaya peel throughout the entire frequency of the pitaya peel sample, dynamic oscilla-
range (0.1–10 Hz). tory results showed G’ (elastic modulus) >
G” (viscous modulus) throughout the entire
frequency range (0.1–10 Hz), showing vis-
obtained exhibited a nonlinear relationship coelastic solid behavior with a gel-like struc-
between the shear stress and shear rate ture. The moduli G’ and G’’ are dependent
(figure 3), thus demonstrating non-Newto- on the frequency (figure 4). According to
nian behavior. Rao, a weak gel presents G’ and G’’ depend-
ent on the frequency and low range
The deviation from Newtonian behavior
between G’ and G’’, like that shown for the
of the solution of ground pitaya peel can be
pitaya peel sample [38].
justified by the presence of insoluble parti-
cles and suspended insoluble materials con- Montoya-Arroyo et al. reported that
sisting of pectin and polysaccharides. pitaya peel has high viscosity values at low
Furthermore, pitaya peel is rich in insoluble concentrations and the shear-thinning flow
fibers (2011 harvest: 2.90 g⋅100 g–1; 2012: behavior showed interesting similarities to
5.70 g⋅100 g–1) which exert a significant that of commercial thickeners like locus
influence on their rheological behavior. bean gum and guar gum [18]. Therefore,
The results obtained with the flow curves pitaya pericarp is suggested to be a prom-
were fitted by power law (Ostwald-de- ising alternative to these commercial gums.
Waele), these being: consistency index (k) = To evaluate their thermal stability, the
131.61 ± 0.80, flow behavior index (n) = ground pitaya peel in water sample was sub-
0.20 ± 0.00 and R2 = 0.99; and Herschel- mitted to temperature variations, from 5 °C
Bulkley model: yield stress (τ0) = 6.36 ± 0.05, to 95 °C at 3 °C per min. Results show

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F.R. de Mello et al.

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Fruits, vol. 69 (5) 389


F.R. de Mello et al.

Evaluación de las características químicas y del comportamiento reológico


de la cáscara de la pitahaya (Hylocereus undatus).
Resumen – Introducción. La cáscara de la pitahaya se emplea como ingrediente alimenta-
rio, ya que presenta betacianinas. Sin embargo sus polisacáridos pueden contribuir también a
la nutrición como agente de textura. El objetivo de nuestro estudio fue el de evaluar las carac-
terísticas químicas y el comportamiento reológico de la cáscara de la pitahaya. Material y
métodos. Se analizaron muestras de cáscara de pitahaya en cuanto a su contenido de hume-
dad y de sales minerales, proteínas, lípidos, azúcares, fibras, vitamina C, acidez valorable,
materias sólidas solubles, así como su pH. Se efectuaron medidas reológicas con un reóme-
tro, para estudiar la curva de flujo, el barrido de la fluencia, el barrido de frecuencia y la
variación de temperatura. Resultados y discusión. Los resultados mostraron que la cáscara
de la pitahaya es rica en fibras insolubles y presenta un comportamiento no Newtoniano,
característica de un gel con predominancia de un carácter sólido. Además, las muestras reve-
laron una resistencia térmica a las condiciones de frecuencia, tensión y temperatura del análi-
sis. Conclusión. Habida cuenta de estos resultados, además de aplicarse como colorante
natural en la alimentación, la cáscara de pitahaya podría asimismo contribuir al valor nutricio-
nal y a la textura de los productos.

Brasil / Hylocereus undatus / frutas / subproductos / piel (vegetal) /


composición química / fibras / propiedades reológicas

390 Fruits, vol. 69 (5)

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