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Spec Part A PDF
Spec Part A PDF
PO P
b
• The sample path length, the analyte concentration,
its medium, its cross sectional area, and most
importantly its extinction coefficient, dictate the
resultant P relative to PO
The extinction coefficient
ε = 8.7 × 10 19 ⋅ P ⋅ As
• Auxochromes:
– Do not absorb in UV, but can shift chromophore
peaks to longer wavelengths (red shift) and can also
increase their intensities.
– Note that both -OH and NH2 have an auxochromic
effect the B band of benzene.
– The auxochromic substituents have at least one pair
of non-bonding electrons (lone pair) that can interact
with the π electrons of the ring (stabilizes the π*
state).
Cut-off Points of
Some Common Solvents
Absorption Characteristics of Some
Common Chromophores