Ks 1 — PSug PURBANCHAL UNIVERSITY a ¥ 2009 Bachelor of Technology in Biotechnology /Fifth Semester /Final ‘Time: 03:00 hrs. Full Marks: 60 /Pass Marks: 24 Enzymology and Enzyme Technology Figures in the margin indicate full marks, Group A: Long-answer Questions Answer THREE questions, Sigesbs tr aii = © A Write down the commercial source of mierobiat enzyme OF Write down the function of papain. How can you Produce papain in the <7" commercial scale? 3x10=30 hat is Active Site? Explain the silent features of active site, Group B: Short-answer Questions Auumor FOUR questions, 5) Defi Dextranase,~ {Qe Give the characteristic features of lyase with EC number. \ Group C: Very Shost-answer Questions Write short notes on FIVE: Ue Isolation of enzyme 5x2=10 » 12. Criteria for purity of enzyme 13. 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What is enzymology? List out the various methods for enzyme Purification. Explain about any one. 2. Write the various application of immobilized enzyme. What are the uses of Biomarkers? Point out the advantages of microbial enzyme. Write the Procedure to find their specific activity of an enzyme, 4. What is thermostable enayme? Describe the isolation of microorganisms and screening of new product for enzyme production. Group B Answer FOUR questions. 4x5=20 f 75. What are the requirements that must be fulfilled in formulating optimum condition for the Production of specific enzyme? 6 Show the linweaver burk plot .of competitive inhibition with suitable examples. 7. What are the factors that affect enzyme activity? Mention the i Kinetics of each factor. “8. Write the therapeutic use of an enzyme, F How do you produce amylase in the laboratory? Contd...(2) 10. What is Isozyme? Mention the type (sub unit) with suitable examples, : ‘Group C Write short notes on FIVE: 5x2=10 11. Allosteric site 12, Bisubstrate reaction 13, Turnover Number 14. Coenzyme 15. Application of enzyme 16. Dialysis 17. Extracellular enzymes RKET. i = ms IRBANCHAL UNIVERSITY apes 2012 Bachelor of Technology in Biotechnology /Fifth Semester /Final Time: 03:00 hrs. Full Marks: 60 /Pass Marks: 24 Enzymology and Enzyme Technology Candidates are required to give their answers in their own words as far as practicable. Figures in the margin indicate full marks. Group A Answer THREE questions. 3x10=30 1. What is enzyme engineering? Write the basic protocol on engineering enzyme. Discuss an enzyme engineering technique adopted to improve a microbial strain. 2eae4 2. Explain changes in body by enzyme: 3. Explain the types of bioreactor using immobilized enzymes. Write the applications of immobilized enzymes. 545 poe the methods of separation of enzymes on the basis of possession of specific binding sites or structural features. Group B Answer FOUR questions. 4x5=20 5. Write in brief on protease and its production method. ( (fc How Xray crystallography help in determining protein structure? Te What is enzyme inhibitor? Write briefly on different types of inhibition observed. 8. Explain the application of enzymes in leather industry. 9. What are the advantages of multiple enzymes upon single ‘enzymes? 10. What are the advantages of microbial enzymes (gr other sgurces? Contd. ...(2) Group C Write short notes on FIVE: an 22 What is the use of enzyme activators? 13, a4. oo 16. a7. 18, What is allozyme? Lit out any two clinically important enzymes, Write any two uses of dextranase, Encapsulation of enzymes Marker enzyme Heat stable enzymes Genetic manipulation of commercial enzymes,RBIAI2SH G_ = KELSO | 2 ae) i \ VG i SS BORR / Bachelor of Technology in Biglahisoloy/ Firth Semester/ Final | ‘Time: 03:00 hrs. Full Marks: 60 /Pass Marks: 24 | BT334E7: Enzymology and Enzyme Technology Candidates are required to give their answers in their own words as far as practicable. Figures in the margin indicate full marks. Group A Answer THREE questions. 3x10=30 1. Discuss, on strain improvement of industrially important microorganisms. 2. Define enzyme inhibition with their types. Also discuss MM and LB plot of each type. 3, What are different methods of enzyme immobilization? Write some industrial applications of enzyme immobilization? 4. Give a general overview of production of microbial enzymes. How do you determine the purity? Group B Answer FOUR questions. : 4x5=20 5. How will you maintain the quality control during preserve industrially important microorganisms? 6. — Give some main features of active site. _/1. How to optimize the chemical condition for enzyme activity? -A, Why microbial enzymes are preferred over plant and animal sources? <6, What do you mean by enzymes as biomarkers? tustrate with a suitable example. __30." Describe enzyme engineering for design of novel enzymes. Contd. ...@ sen Group C : 2 Write short notes on any FIVE: — *5x2=10 LL, Regulatory enzymes 12. Define Zymogen with examples. 13. Metalloenzymes 14, Differentiate Coenzymes and Cofactors 15. When you eat pineapples, pineapples eat you right back. Explain. ‘6. Industrial application of lipase and pectinase ear eaten 418. Patenting enzyme producing organism iS tBachelor of Technology in ‘Time: 03:00 hrs. Full Marks: 60 /Pass Marks: 24 | _BTS34ET: Enzymology and Enzyme Technology Candidates are required to give their answers in their own words as far as practicable Figures in the margin indicate full marks, Group A Answer THREE questions. ‘ 3x10=30 1, Write in detail the isolation and screening methods of organism for enzyme production. 10 2. Give the processing mechanism for extraction of the extra cellular enzymes with diagram. Also focus on feedback inhibition with examples. 743 3. What are the thermo stable enzymes? How do you screen organism producing thermo, stable enzymes? Write down the application of these enzymes in industries and in brief explain the methods of processing the strains. 2+3+5 4. Describe the various methods of immobilization of enzymes with suitable diagram; also mention the advantages of immobilized enzymes. 743 Group B Answer FOUR questions. 4x5=20 5. List out the various commercial enzymes used in detergent. ‘And explain their activity. 5 6. Explain the procedure to design and construct novel enzyme. 5 7. Mention the production protocol of commercial enzymes (amylase & Papain) 2.5+2.5 8. What are the advantages of microbial enzyme as compared to other? 5 contd.(2) 9. Differentiate between biocatalyst & chemical catalyst 10. Give the various salient feature of active site, . 5 ” Group C Write shért notes on any FIVE: 5x2=10 ' 11. Purification of enzymes. : 12. Regulation of enzyme activity : 13. Specific activity of an enzyme. 14. Role of allosteric site. 15. Improvement of strain. ' 16. Enzymes in therapeutics i 17, Plant & animal source of enzyme ; 18;-"Tumover mutiber i i[techniques used In enzyme technology « é 30: EEDeHmemact isi: Answer three questions : sp shen Be -|€xptain the _process of selection Of Wuutants for production — Cp fonmersay valuable SOE Unes: fi. sth Sewester QO16 Enzymology ond Enzyme Technolog \Give the Vasious process that involved in re guistion of Bxio= 30 so to |Specity the various _|leather tndustry. ~ Gissup-b [Answer jour questions : , : [Brier ‘describe about the isolation |Soeplain ol patenting | tuieroorgas 7. LOrite in detail about the vasious wettede of immobili- borite in detail about the industrial application and pecduction protocol Of Rextranase and Pectinase. StS f use = Uxs 220 y nd pusigication e | = au 7 |bOrite. He advantages oR blocatatyst upon Chewical : egret sins < rol “enzyme De. ee sent eee © op — Sin de ces one a Mention he wiechanisu Of _Processin Qnd Uberation of extraceliulan enzyme. + : St eSHow enzyme engineesing play. commercial appiication? Lorite Shost notes on any five: gt | Thermostable enzyme Bioreactor .| Metal activated enzyme Selection o Sources OF ENTYME ipevnous curve | TUBMB Specigic activity [Inducible enzyme rs > vital vole duzing its RS SxX2=10séuanoe aUiAzu9 Ut UO! 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Pull Marks: 60 /Pass Marks: 24 BT334ET: Enzymology and Enzyme Technology Candidates are required to give their answers in their own words as far as practicable. Figures in the margin indicate full marks. Group A Answer THREE questions. 3x10=30 1. Discuss the different methods of strain improvement. 10 2. Define immobilization technique and its application in industry, What are the used of Biomarkers? TH 3. Discuss the applications of Microbial Enzyme. List out the enzyme purification techniques. B42, 4. Define Lineweaver Burk ‘plot. Give detail about the type of inhibitor with example. 37 Group B Answer FOUR questions. 4x5=20 5. How weak interactions between Enzyme and Substrate help to decrease the activation energy during Enzyme-Substrate Reaction? Define kat /Ka? Derive it from Michaels-Menten reaction. Define feedback inhibition of enzyme with suitable examples. Point out the feature of Catalytic site on an enzyme. yexne Write down about the random scfeening method for selection of mutant. 10. Write down the function of papain. Contd. ...(2) Group Write short notes on any FIVE: Sx2=10 11, Multi-level screening method 12, ts0eyme 13. Co-Enzyme and co-factor 14. Scale-up 15, Lipases 16. Fermentation technique 17. Active ste u