20 andbok of COWO-19 Prevention and Hestmert © Detection of Serum Antibody Specific antibodies are produced after SARS-CoV-2 infection. serum antibody determination methods include colloidal gold immunocrvomatogrenhy, LISA, themiuminescence immunoassay, ete. Positive serum specific igM, oF specific IgG Sntibady ter nthe recavery phase 24 times higher than thatn the acute phase, eon BS Used as dlagnostic criteria for suspected patients with negative nuceic acid detection During follow-up monttoring, igM's detectable 10:days alter symptom onset and IgGs detectabie 12 days alter symptom onset. The viral load gradually decreases ith the Inetease of serum atibody leves O detecting indicators of inflammatory Resaonse It ig recommended to conduct tests of Creactive protein, procalctonin, ferrin, Dedimer, total and subpoautations of imphocytes, if IL-6, I-10, TNF-a, NF-Y and ther Indicators of inflammation and immune status, hich can help evaluate elniesl progress, alert severe and crical tendencies, and provide a basis forthe formulation of treatment stategies Most patients with COVID-19 have @ normal evel of procalctonin with significantly Ineteasea loves of C-reactive protein rapid and significantly elevated C-reactive protein Level Indicates possiblity of secondary Infection. D-dimer levels are Eigniicanly elevated in severe cases, which sa potential ise facior fr poor prognosis Patients with a low total number of lymphocytes at the beginning of the disease Generally nave a poor prognoss. Severe patients have a progressively decreased humaer of perioheral blood lymphocytes. The exaression levels of -6 and IL-0 in Severe patents are increased greatly. Moniioring te levels o'l-6 and 101s Melprulzo {353055 the risk of progression toa severe condtion © etecsion ot Secondary sacterial or Fungatintections Severe and critically Wl patients are vulnerable to secondary bacterial or fungal intections Quaitieg Specimens should be cllecced from the Infection se for bacteral fr fungal culture. secondary lung Infection Is suspected, sputum coughed trom deep In the lungs, wacheal aspirates, broncnoalveoiat Lavage fluid, and brush specimens should be collected for culture Timely blood culture should be performed in patients with high fever 8lood cultures drawn from peripheral venous or catheters should be performed in patients with suspected sepsis who had an indwelling catheter. Is Fecommended that they take blood G test ad GM test a east twice a eek in addition tofungat culture O Laboratory Safety Biosafety protective measures should be determined based on diferent sk evels of experimental process. Personal pratection should be taken in accordance with 85.3 Ianoratory arotection requirements for respiratory act specmen ealection, nucle eid detection snd virus culture operations. Personal protection in accordance with BsL2 laboratory protection requirement should be carried out for biochemical immunological tests and other routine laboratory tests, Specimens should be transported in specal transport tanks and boxes that meet biosafety requtements. Al Ihboratory waste sould be stiety autoclaved.