ene SA - 6€ VI Semester B.Sc. Examination, April/May 2015 (Semester Scheme) (Fresh + Repeaters) BIOTECHNOLOGY - VII Plant Biotechnology (70 marks - 2013-14 and Onwards/60 marks — Prior to 2013-14) 3 Hours Max. Marks : 70, Instructions ; |) Draw neat labelled diagrams wherever necessary. 2) 70 marks for students 2013-14 batch Onwards. 3) 60 marks for repeaters prior to 2013-14 batch. 4) Q. IV, Section D is compulsory for students of 2013-14 batch onwards. SECTION-A |. ‘Write short notes on the following (Sx2= 1) Expiant 2) Dadifferentiation 3) IPR 4) Binary vector 5) Qvule culture, SECTION-B |, Answer any four of the following : (4x5: 6) Give an account on cybrids. 7) Write a note on surface sterilization of explant. 8) Explain the role of tissue culture in agriculture. 9) Explain edible vaccines in watermelon. 10) Explain microprojectile technique as aSA-655 SECTION-C (310230 Iti, Answer any three of the fallawing : ; 1) What are secondary metabolites ? Explain the production of alkaloids in vitro, 12) Describe the method of shoo! tip and callus culture. Add a note on its applications. 13) Briel the applications of ransgenic plants 14) What are protoplasts ? Exptain isolation of protoptasts from leaf. 15) Expiain a) TRIP's b) Trade secrets. SECTION~D (From 2013-14 batch onwards} IV. Answer Ihe following in one word or a sentence each : (10x1=10) 16} What is endosperm ? 17) Expand PEG. 18) Whats hybridization ? 19) What are biobe elicitors ? 20) What is electrotusion 7 21) What is meant by organogenesis 7 22) What is culture rogm ? 23) Name the instrument used for dry heat sterilization. 24) Name two cytokinis. 25) Whats tapeturn' ?mee Vi Semester #Sc. Exuniination, May/June 2010 (Semester Scheme) BIOTECHNOLOGY - VII Industria! Biotechnology Tiree ¢ 3 Hours ‘Mex, Mares 60 > Jitrection: Drde désgram wherever noo tary SECTION - A {, Ancucr any five of che following (Ex2=10) iy Safin, er oa by Raeon —<—<—<$<$<—<$<$_$__— SE 7); Salid state formersation 4) Yoghast. © 5 ve 6) immobilization. SECTION - 8 IL Aniwer any four-of the following (4e5=20) 7) Describe [nduscrial production of cite acid Hi Give w detailed sceount on bioreactors for eazyme-production. 4) Gieran account on strain improvement srutegies. \ (0) Sristiy commers on the production af fermentad fords with twa eaumptes 11) Explain the production of pemiillit:: monn eM = 253 SECTION = C (3e10= Ih, Answet any three of the followin. ‘ production of Fleer 12) Gives detailed account ae in 13), Describe the step Involved in down stream processing (DSP). 14). Explais the differenttypes ot! fermentor. (5) ‘Deseribe che industrial preduction of enzymes. ‘Add wnote on theiruses. 16) Explain'the production of A) Xsan cum By Biopolymer. aVi Semester 8.Sc. Examination, May 2016 (Semester Scheme) (Fresh + Repeaters) (70 Marks — 2013-14 and Onwards) (60 Marks — Prior to 201 9-14) BIOTECHNOLOGY — VII Plant Biotechnology Time | 3 Hours Max. Marks - 70/60 Instructions: 1) Draw neat labelled diagrams wherever necessary: 2) 70 marks for students 2073-14 batch Onwards. 3) 60 miarks for repeaters pnor to 2013-14 batch 4) QLIV, Section = Ds compulsory for students of 2013-14 batch Onwards, SECTION-A 1. Weite short notes on the following : (Sx2=10) 1) Surtace sterilization 2) Aurotrophic mutants 3) Endesperm culture 4) Patent Co-operation Treaty 5) Subunit Vaccine SECTION-B 1). Write any four of the faliowing (425=20) 6) What fire reportor genes 7 Write a note on luciferase gene expression 7) Write the significance al Nil-gene transfer through tissue culture 8) What is osmosis 7 What Is the role of osmoticum |n protoplast isolation 7 9) What Is the advantage ‘of pollen culture over anther culture 7.Give 3, applications of anther and pollen culture 10) Write a note on cytokinin. Give two examples of synthetic cytokinins. P10.LT. MS-344 2 SECTION - G 10=30 WL Answer any three of the following Li , 11) Detine Somatic Embryogenesis Explain in dotall its procadure and significance. 12) What are the diferent ways of protecting IPR ? Write a note on Plant Beaders Rights. 19) Whatis T-DNA ? Describe the construction af binary vector system. 14) Whatis-ovary culture? How is it done ? Write 5 applications of iL 19) Describe the mechanism of somactonal variation, How are somacional variants selected ? SECTION -D (From 2013-14 Batch Onwards) 'V. Answer the Intloving in one word or a sentence each (10x10) 16) Select the correct answer "Potassium is a mactonuirient in Nulrient Media a) Fatse b) True ¥7) Give the other name ol ‘Qvuis’ 18) Which expiant is ised for propagation of orchid plant Nettie nidus-awis 7 a) Root b) Shoot tip ©) Inflorescence: 19) Which Indian scientist first rapomed development ot embryo trom mikrespores. of Qatura Innoxia,? a) ManesnwanS.C. b) Moret. ce} White P & 20) What are tusogens 7 21) Potatalate blights causedby 22) Choose the comect answor; a) RIA Is Rackal Immunologial Assay b) RIA ts Radio immuna Assay 23) What are phytoalexing 7 #4) __gene was isolated trom Aaquores Victoria a vatioty ot pally tints a) ofp 5) lue 6) X-gal 25) Expand TRIPS.r yc sM-4 Vi Semester B.Sc. Examination, May/Ju ' Se. 7 ne 2018 ‘BCS) (2016-17 and Onwards) (2013-14 and Grnderdsh (Fresh + Repeat BIOTECHNOLOGY - Vil Plant Biotechnology me: 3 Hours: Max. Marks Instruction - Draw neat labelled diagrams wherever necessary. SECTION -A |, Write short notes on the following oe 1) G. Haberiandt. 2) Hairy real culture. 3) Somaciones. 4) Acclimatization, 5) Endosperm. SECTION -B i) Write any tour of the following _&) List the applications of pollen culture. 7) Write a note on the applications of Micropropagation _-8} Discuss the importance ‘al organ culture 9) Explain the types of protoplast culture AG) What is a patent 9 Mention its fimitations. (ax SECTION -C ii Answer any three al (he following (3x 11) Discuss in detail the procedure for invitro ambryo cullure. Add a note on significance 12) Whal are phyloaioxing 7 Biedeet 4 iin f Bl PRR 6 industrial production of shikonin.=, SM 407 Wa 13) Write in detail about invitra somatic embryogenesis. 14) Describe the microprojectile method of gene transfer with a diagram. Adda note on its disadvantages 15) What are ediblé vaccines 7 Discuss in detail its production with a suitable example. SECTION - 0 IV. Answer the following in one word or a sentence each © (10x1= 16) What is hypochlorite 7 17) Name any two commonly used vitamins in tiusue culture media. wren! | 18) Expand BAP 19) What are somatic hybrids 7 20) Expand TIP. 21) What is fluorescein diacitate ? 22) Doeline explant. 23) Expand TRIPS, 24) What is acetosynngone. 25) Deline dedifferentiation.Fre" muna g-362 "vi Semester B.Sc. Examination, May/June 2019 BIOTECHNOLOGY-VII Plant Biotechnology (CBCS) (2016-17 and Onwards} (2013-14 and Onwards) (Fresh+Repeaters) me ; 3 Hours Max. Marks 70 structions to Candidates : Draw real labelled diagrams where necessury SECTION - A x21 _ Write shart notes on the following = Za 24-0 2. CPW 3. Trade Secreto 4, Encapsulation 5. Embryoid : SECTION - B n. Answer ony four of the following © 4x5" | Weite une aseptic: techniques dined in Plant Tissue Culture 7, texplain the mechanism and applications of gametoclonal variauion. | What is ovary culture ? Discuss itn protecol Describe in detail, the procedure of protoplass isal ad. Comment on Copynght and Traderiark.8-362 Th. Answer any three of the following, < % 14. 14, SECTION - C Sx10%: What in tetrad ? Give an account of haplaid culture with ile applications. What are elicitars ? Explain the induntrial applications of necondary metabolites. Define transgenit plants, Dincuss Ajrobactenum mediated gene transfer, Write a note on ‘Somatic hybridization’ and ite applications in detail, Dinowsn the rote of Tissue Culture in Agriculture and Forestry with suitable examples. SECTION - D Anewer the following in ome word or a sentence cach ws. a. Ethylene 1Ox1=! Synthetic media ‘T-DNA Expand POR Hardening Fusogent . Triploidy: Kahamyein Rexintant gene Embryo rencuc Hetergkaryan -e0o-> ame SM —408 ‘Vi Semester B.Sc. Examination, May/June 2018 (CBCS 2016-17 and Onwards/ 2013-14 and Onwards) (Frosh+Rapeators) BIOTECHNOLOGY Industrial Blotochnology (Paper — Vill) Time | 3 Hours Max Marka 7O Jastructions © Oraw neat jabollod diagrams wherever nucussary. SEGTION -A |. Write short notes on the following {Sx2=10) 1) Baiting 2) Upstream processing 3) Product extraction 4) Biotranstormation 5} Membrane reactor. SECTION - 8 || Answer any four of ine folowing (45220) 5) What are microbial polysaccharides ? How are they produced ? 7) Describe ihe types o! balfies Lised in farmanters. 8) Explain the uses of enzymon in !aather processing indusines, 9) Write a note on canicillin production. 10) Explain vatiows methods inwolved in concentration of products in DSP. SECTION -C fl, Answer any three of ihe following (2 10=30) 11) Explain various sterilization techniques 12) Give an account of strain improvement 13) Discuss the use of immobilised anzymos in upstoun (AU 14) Descrite industrial production of clirie acid. 18) Write noton on . a) Stirred tank b) Airlift fermenter. SECTION =D IV. Answar the following in ane word or a sentence each 16) What are polyostars ? 17) Expand SCP 18) What is whoy 7 19) Detine coagulation, 20) Name a steroid produced by blotransformation. 21) What is a fermonter 7 22) How is foam controlied in a fermenter 7 23) Nama a microbial polysaccharide. 24) What is malting of bear 7 25) Name two anzymes used In tood industry. aocme | chal ve MS = 204 VI Semester U.S. Bxvilnation, May 2011 (Semester Scheme) RIOTECHNOLOG ¥ (Paper = Vit) Industrial Bioweetinalogy Max, Marks: 40 Time: 3 Hours Instruction: Drew meet labelled dlagrins whereret necensniry. SECTION =A 1. Writ short notesen the follwing? (Sx2=10) 1) Natura) medium. 2) Autoclsving j 3) Proteases in food processing: ' 4) Balting twebnique. 5): Pilot plant. SERTION ~ Bt (4x5=20) H Answerany four ofthe following | 6) Describe the mutant selection method of strain improvement. 7) Explain different types of sibmerged fermentation. §) Give an account of the techniques used in concentration of the product 9) Explain the production of Xanthan germ. Add a note on its importance. 10) Write an account of pl i iio of plant cell suspension culture in the tion of food P.O,M368 TTA, OL Antwer any three of the following (3e10=30) 11) Desersbe the design af Airlift fertjecitir with'o deat labelled diagram. 12) Deseribe the different steps intel ved in the industrial production of Penicillin 13) Explain the industrial process of production of citric acid. 14) Explain - a) Production of Yorurt ‘b) Tower fementer, TS) Givean Wcrolnt of malehial prea aoH obmivine. — ~ |noe MS — 345 Vi Semester B.Sc. Examination Se. , May 2016 (F + A) (F — 70-2013-14 and Onwards/R - 60-Prior to 2013-14) BIOTECHNOLOGY (Paper — Vill) Industrial Biotechnology 10:9 Hours Max, Marks : 70/60 Instructions : 1) Craw diagrams wherever necassaly. 2) 70 marks for students of 2013-14 batch Onwards. 3) 60 marks for repeaters Prior ta 2013-14 batch, 4) Section - D, question IV is compulsory far students of 2013-14 batch Onwards. SECTION-A Write shart notes on the following: (5x2=10) 1) Lyophilization 2) Hops 3) SCP 4) Immobilization 5) Membrane bioreactor. SECTION—B it Answerany four ol the following - (4x5=20) 6) Briefly explain the process of production and recovery of penicillin. 7) Weita a note on the importance af microbial enzymes in food and beverage inciustry: 8B} Describe the industrial strain improvement by (DNA method. 9) Explain in brief the steps involved in the recovery 10) Write'a note on microbial polysaccharides. \\s PTO.SECTION -C Answer any three of the following : 1 and its 11) Give a detailed account of the importance of media componen formulation in fermentation tat 12) Explain the salient features of surlace, submerged and solid state fermentation, Add a note on their advantages and limitations. 13) Write a detailed account of the production of various food additives. 14) Describe the different steps involved in the Industrial production of citric acid. 15) What are fermented foods ? Explain the production of Yoghurt and Cheese. SECTION-D (For Students of 2013-14 batch Onwards) Anawer the following Inone Word ora sentence each : 18) What are steroid hormones ? Give an example. 17) What is tlash pasteurization ? 18) Mention two aw materials for ethanol production 19) Whalis dual fermentation > 20) Mention ‘One organism usad in Induatrl 21) What is Idiophase ? 22) Which one of the following is called a5 the ‘brewers yeast’ 7 8) Saccharomycas ludwigi b) Saccharomyces cerevisiae ¢) Saccharomyces boutardy 9) Saccharomyes Pastorianus, 23) Which of the following is used for agitation ina fermentor? 8) Impeller b) Batfies ¢) Sparger d) None of these (10%1=10) lal production of Vit. By 2Mi MS = 32 Vi Semester B.Sc, E: .Sc. Examination, May/June 2014 ter Scheme) (F-70 ~ 2013-14 and onwards/R-80 ~ Prior to 2013-1 BIOTECHNOLOGY (Paper — Vili) BTP 603 : Industrial Blotechnology @: 3 Hours Max. Matks : 70! Instructions: 1) Draw diagrams wherever necessary. 2) 70 marks for students of 2013-14 batch onwards. 3) 60 marks for repeaters prior to 2013-14 batch, 4) Section D, Question IV is compulsory for students of 2013-14 batch onwards. SECTION-A Write short notes on the following nee 1) Baiting technique 2) Autoclaving 3) Rotary drum vacuum filter 4) Roquetort cheese 5) Sparger. SECTION -8 Answer any four of the following : (axed 6) Describe the industrial strain improvement by (ONA mathod. 7) Give an account ol techniques used in disruption of microbial cells in OSP- 8) Explain the uses of capsaicin. 9) Write a detail note on microbial polysaccharides. 10) Explain the concept and application of solid state fermentation SECTION-C Answer any three of the following - (3x IC 11) What is a fermenter 7 Explain the design of an air-lift fermenter. 12) Describe the process af industrial production of beer.MS-321 13) Wite a detail account of microbial prodection of glutamic: acid. 14) Explain the mechantim of microbial steroid biotransformation with @xan pees. 15) What is single call protein ? Explain the mathod at production af SCP SECTION-D (For students of 2013-14 batch onwards) IV. Answer the following in one word ora sentence each (10«1=10) 16) Namo the inducer used in the medium for the production of penicillin. 17) Name one species of organism used in industrial production of vitamin By» 18) What is the use of baffles in a fermenter 7 19) Whal are secondary metabolites ? 20) Whal are hops used in beer production? 21) Name a microorganism which hydroxylates cortexolone, 22) Write the full form of PHA. 23) What do you mean by red wine ? 24) Why synthetic media is also called as defined media ? 25) What is lyophilization 7100519 hv of Prutest Pay (NAO NU GS-363 VI'Semester B.Sc. Examination, May/June - 2019 BIOTECHNOLOGY Industrial Biotechnology (Paper-VIl) (F+R) (CBCS-2016-17 & Onwards/2013-14 & Onwards) ‘Yume - 7 Heures Max. Marks | 70 Instnictions : Drau nat fabello! diagrurn whereuer locessary. SECTION - A 1, Wrine short utes on the fallawig, 5ez=10 Ay Liquid Nitrogen prescrvation 2. Limitations af sutmerged fermentanon 3. Semi nynthetic penseillins 4. Continuous Mow reactor 8. Yoqurt SECTION - B Pil, Ankwer any four of the following 4xS=2 “6. Invprovement of strains by re pmbinant BNA techinelogy 7, Write 4 nate on heat sterilization techniques. SB. Explain single cell Gil, we Give an neeount of aynthetic media. 10. Dexeribe emxyme inmubilization,163. Anawer any threw of the follewin al. wi, us 14. SECTION -C 3x10=2 Explaim int detail the steps involved! in production: ef Xanthan yur Add a note on ity apphealans Give an account of down stream pracessitiy, with reference (0, {a} Solvent recovery fey Cell disruption. Discuss in detail about mictabial enaymen and add 4 note on its applications im leather und deteraett industries. ‘Explain the different type of spargers and impellers. Add 4 note on its advantages. aS. Describe in detail the production of Glutamic acid and Vitamin By SECTION - D Answer the following in one word or @ sentence cach 10x1 46, What is tophophane 7 47. Define strain. is. Write any two Taw tnaterials used in solid state fermentation. us? What ase vintifoain agents ? 96, Define crystallisation @1. What is layering ? a, Name the strains involved in Ili fermentation 23. What is benzyl penicillin ? 24. Expand PHB a. What i Molasses ? -s0o-US - 391 vl Semester 8.S¢, E: (CBCS ~ 201617 xamination, May 2017 & Onwards/2013-14 & Onwards) (F + R) BIOTECHNOLOGY - vu Industrial Biotechnology ne: 3 Hours Max Marks 70 Instruction: Oraw neat labelled diagrams wherever nacessary, SECTION—A . Write short notes an the following 1) Inoculum and its importance, 2) Bioreactor. 3) Antifcam agents. 4) Yoghun. 5) Polyesters. (5a2=10) SECTION-B IL. Answer any four of the following : 6) Describe the mutant selection method of strain impravement, 7) Expiain in detail about bubble column bioreactor, 8) Write a detaiied account on the production of PHA. 9) Write the various steps involved in the production of Vitamin B,. 10) Expiain the importance of microbial enzymes in food and detergent industry, (4x5=20) SECTION -C I Answerany threeo! tha following © 71) Define fermenter and explain different control systems in farmentar. 12) Explain in detail the techniques used in separation, extraction and oncentration of products. 13) Discuss various industrial applications of 14) Explain in detail about the industrial 15) Write a detailed account of aleoholic (310230) ero.Us-301 (atin SECTION-D 'V._ Answer the following in one word ora sentence each : tal) 18) Mention any one organism used in amylase production, 17) Detine biotranstormation, 18) What is pasteurisation ? 19) Name the microorganism used in industrial production of glutamic acid. 20) What are hops ? 21) Who discovered Penicillin ? 22) What is Beer wort ? 23) Define lyophilization, 24) What is molasses 7 25) Expand HEPA,jn Ms -321 VI Semester B.Sc. Examination, May/June 2014 smester Scheme) (F-70 - 2013-14 and onwards/R-60 - Prior to 2013-14) BIOTECHNOLOGY (Paper - Vill) BTP 603 : Industrial Blotechnology me ; 3 Hours Max. Marks : 70/60 " Instructions ; |) Draw diagrams wherever necessa/y- 2) 70 marks lor students of 2013-14 bal cowie 3) 60 marks for repeaters prior to - [ 4) Section D, Question IV Is compulsory for students of 2013-14 batch onwards. SECTION-A Write short notes on the following © {5x2=10) 1) Baiting technique 2) Autoclaving 3) Rotary drum vacuum filter 4) Roqueton cheese 5) Sparger. SECTION-B Answer any four of the following : (ax5=20) 6) Describe the industrial strain improvement by rfONA method. 7) Give an account ol techniques used In disruption of microbial ceils in DSP, 8) Explain the uses of capsaicin. 9) Write a detail note on microbial polysaccharides. 40) Explain the concept and application ‘ol solid state fermentation. SECTION-C. Angwerany three of the following : 11) What is a fermenter ? Explain the design of an air-lilt fermenter. 12) Describe the process of industrial production of beer, (3x10=3 Pa(a MS-321 13) Write a detail account of microbial production of glutamic acid. 14) Explain the mechanism of microbial steroid biotransformation with examples. 15) Whalis singia call protein 7 Explain the method of production of SCP. SECTION-D (For students of 2013-14 batch onwards) 'V. Answer the following in one word or a sentence each : (0x1 16) Name the inducer used in the medium lor the production af penicillin 17) Name one species of organism used in industrial production of vitamin By: 18) Whal is the use of baffles in a lermenter ? 19) What are sacondary metabolites 7 20) What are hops used In beer production 7 21) Name a microorganism which hydroxylates conexoione, 22) Write the tull form al PHA, 23) What do you mean by red wine ? 24) Why synthetic media is also called as detined media 7 25) What is lyophilization ?ai US — 391 Vi Semester B.S¢, Examination, May 2017 (Fresh+Repeaters) (CBCS — 2016 - 17 & Onwards/2013 — 14 & Onwards) BIOTECHNOLOGY - VII Plant Biotechnology he: 3 Hours Max, Marks © 7 instruction: Draw neat labelled diagrams wherever necessary. SECTION-A Write shart notes on the following : Bx2ntt 1) Callus 2) Meticiones: 3) Vegetative propagation 4) Intellectual property rights 5) Reporter genes. SECTION-B Write any four of the following. (4x5=2€ 6) Describe the tactors atfecting micropropagation. 7) Explain the role of auxins and cytokinins in plant tissue culture. 8} Give an account on the selection af somacional variants 9) Describe the methods of isolation of protoplasts. 10) Write a note on trade secrets, SECTION-C II. Answer any three of the following. Bx10=36 11) Describe the procedure of anther culture. Add a note on its applications. 12) Define secondary metabolites, Describe the methods o! production af secondary matabolites. 13) Describe the methods of protoplast fusion. Write a note on the selection at somatic hybrids. 14) Describe the applications of plant tissue culture in the field of forestry. 15) Whatis Agrobacterium madiated genelianeter ? Explain the types of vectors basedon Ti-piasmid. ie;- 390 mums SECTION-D Answer tha fellawing in one word or a sentence each. (10st 16) Expand HEPA. 17) What are elicitors ? 18) Name the fungus trom which gibberellic acid was discovered. 19) What are heterokaryons ? 20) Name the disease caused by Agrobacterium tumetaciens. 21) Define somatic embryos. 22) Mention any one of the limitations of edible vaccines. 23) What are synthetic seeds ? 24) Name any two enteria for a plant varlaty to be recognised under PBR. 25) Whatis GATT?Ms - 320 Vi Semester B.Sc, Examination, May/June 2014 Semester Scheme) (F-70-2013-14 and Onwards) (R-60 Prior to 13-14) BIOTECHNOLOGY — Vil BTP — 601 : Piant Biotechnology ime : 3 Hours fastructions : 1) Draw neat labolled diagrams wherever necessary 2) FO marks for students 2g73-14 batch onwards. 3+) 660 marks for repeaters porto 2073-14 bare! 4) 0. IV Section Sermpanory for suri (2073-14 BHC onwarrcls. SECTION-A 1 Write short notes on the loflowing ~ (5x2=10) 1) Gallus 2) Edible vaccines 3) Copyright 4) Regeneration 5) Ovary culture. SECTION -B ii. Answer any four of tho following : (48220) §) Give a brief account of Auxins. 7 exptain cybridization. Adda note on its significance. 8) Describe the isolation of protoplast by chemical methods. a istinguish batween invive and jnwitra fertilization. 10) besrba the apteatans otanperc ans nar PTO.~ ia Uy SECTION -C Ill, Anawor any three of the following. (3x10=30) 11) Deseribo somatic hybridization, Explain its applications. 12) Whatis somactonal variation ? Explain the mechanism and signilicance. 19) Deling organ culture, Elucidate the process of embryo culture and add a note on its applications 14) Explain the imporarice of intelectual property rights in blotechnalogy. 15) What It clonal propagation 7 Describe the methods and applications. SECTION—D (From 2013-14 batch onwards) IV. Answer the follawing in one word ora sentence each (10x1=10) 46) What is totipatency ? 17) What are growth regulators ? 18) What is the explant used forhaploid production ? 19) Wiite an application af PTC in forestry. 20) What is de-dilterantiation 7 21) What are wade secrets 7 22) What do you maan by an explant? 23) What ara synthetic seads ? 24) What are elicitors 7 25) What is acclimatization ?+j S$ - 260 ‘OEELETE A A VI Semester B.Sc, Examination, April/May 2012 (Semester Schome) BIOTECHNOLOGY ~ Vil Plant Biotechnology Time) 3 Hours Mae Marka 60 Jnstruction : Drew a neat labullie diagram wherever hocuunairy SECTION =A |. Anaweor tho following (Sadt=10) 1) What is meant by 1HIPS 9 2) What are vir ganas 7 3) What is invita rooting ? 4) Name any twa vitamins used in PTC 5) Distinguish between invited and invive fortilization SECTION-B iL Answor any four of the following (445220) 'B) Explain the infivance nutnents in Secontary mutabolite production 7) What are edible vaccinats ? Explain the production af vatcinves in banana. 8) Give an account at Application of tissue culture in horticulture 9) Give an aetaunt of mictoprppagation through muristern ‘CUlLUEE and communi ‘onthe significanen of tin agriculture: 10) Describe any two applications: Of transgene plant\S = 260 SECTION-C lll. Answer any three of tha following = (3x1 11) Elucidiate the method ol isolation and regeneration of protoptast and add a note on viability test. 12) Describe the various aseptic techniques used in PTC. 13) Whatis [PR ? Mention the main forms of IPR protection and explain patent 14) Whatis endosperm culture and embryo culture ? Write the procedure for the same and add a note on thelr applications. 15) Describe the. genomic composition of T-DNA and the mechanism of its integration inta plant genome.Vi Semester 8.$c. Exeminstion, April/May 2012 (Semester Schema} BIOTECHNOLOGY (Paper ~ Vill) industrial Biotechnology 42°93 Hours. AS — 261 Max Marks: 62 inetruction: Oraw neat lsbolleg diagram wherever necessary. SECTION-A . Write short notes on the following 1) impeiiers 2) Chesse 3) Polywaroxyalkoncidas: 4) Pasteurization 5) Tharmopiies, SECTION-5 Answer any four of the following 6) Write a note onfarmentation media. Th How enzymes are usalul in food and pharmaceiltical industry ? 5) Briefly explain recovery of products in fermentation. 9) Explain ciferem typas of continuous fermentation. 10) Desctiba ihe mass culture of epitulina. SECTION=< Anavear any three of the following: +1 12) af) 44) 15) Give a detailed account on characteristics of snzymaa Explain differant steps in production of Beet Substantiats cifferent types of formeniars with thelr applications. Sriatly explain screening and isolation of microorganisms. Desorde Ihe production of fermented food and discuss the mis of Microorganisms in the process: (Ex2=10} (4n3=20) (gx10=26 ——————————