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Issa-Zacharia £1, Abie EAR L Ca EAR HPAI CRM L 7A eS ALD ETA BHR Ze < 14 AM) pH & ACC ik, Staaten & L-COmuabH (pHS~pH6.5, ACC:10ppm~30ppm) Z#EFF TS Sb MALTS, EOL SRWREMEAT, BIC, Blas AZaAT IW (CMB) HAUNT A-BRY AT A ZOASS I (CNMB) “CHAR HE & ORHEIS LT Yim (OOF, CMB fHRPeDkilins & CNMB @ifRHE aE ARE DEBR) EM L-C, 10C~ 60 COMETS bab EEE E CORTES MSIL IA, ARRON RRELEBDKOTRETS, 2. MRS LOGE FIT FIAF—T3 (HAR, HH be =A AIRSE) “CARURE L TC FERIA BIR (ACC:7,000ppm) Orkiti A ARIK (ACCH#ISOppm, pHB.4) 1C, MNEERE BRI YS SEAM (Fea 7 V-ATSON, OA LTH) ANU, BER QNLimin) SUVIBEAE HA (QNLimin) EBEHAA (NUmin) ZH 7 ER Ge: T24-0816 HARM RV MRA 2325-25 ‘Te: 085-365-8566 E-mail: s_griseus@work adn nejpPOCA LR, WD BAUER) ZAC L Ty PAD OST I-A DCMBPRREEIKIIZK & CNMB. ‘RINE HK Ze BR Lt, PARR HTK ACB CAR fe, FUR CHRIS (OL, LIne RE ARENPER) OHIRLIE. 1 (CACC:48ppm . pHS.2) . CMB ci He ak. CACC:Séppm . pHS.8) EAL Me (Be IE He cB ok (ACC:S3ppm, pHS.7) % 2LAFELD~Sy bak bate ENEMA FEL, 10C, 20°C, 30, 40°C, 50, COTO (TRF 7B, C1410, HER) WRELT, ACCE pHIRO REMAKE RIE Lit. BENE LACCHIEREAQ-102P CEN EHPER) pH SK-620PH (FGRHRRBWUIEFO) 200A Lit. Hitens DOWABAINS BAPE ERU-3210 GBF SEAT FAR) FP SFIS 7 EME AT SLMIOTM (F794 DED SHIH LTE 3. RRL BR 1) CMB RARE MCT ose CMBR HAA ACCHRAFAB & pH HELO ERE, Voit ALE C90 FI NIRARIIEEHOUVBUR ASI byl (62, 3) Dateik, PREP REI CHINKC IRM FeB) 1, 236mm HFC UAB 3 RHE PHOS (CAME S &, onmbhiioowibeA We L C2920 SEI MERWRWOR EL BITE. BUT, pHOSICAIME LIE ma: apt. s & WME FENN CAR CHM LL. 10°C ~ 200-314 & CpHS 8%, 30°C~40°C- 314 Hl & TpHS.9, 50°C + 3I4EGCpHH6O, 60°C + I9SELCpH62e RL (ED) . SiR C314 BREE LCP OUR 2 bovis, 2°CS0C + 314 RE RRIROUVERL AS bob (BM) LIC CH B= Lan, SCA P LAME LIE Lit, SILO HE RINS , CMBItEN EA HAIL, 4 COLES FC REMIND HEH b> ete Lit ence 208 ieee sence 0 pKa as pho oe TPE oH 2 asanwwess 28 8 8 ecw FL. 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FARGIC Seis L 7 SLBA aA OREM ETE A ARGEREMIIOR Lie, ACCHERASIL, ILIA RYIC BUDL, 10-7 BIZ50.2%, 20°C-3 A HKICHS3%, 30°C-2F141=50.9%, 40°C-1 4RIT43.3%, 50-1 (€33.3%, 60'C- 1 FLAEIC32.1% Ca oF, HII, ACT PERM ASE LV S0'C~ OO CERTAIN CHIEF LIE BS, 1l0C~30 CRRA THIEL AUR BavitAvo ee BIC, CNMB PARE ATE MB IEHEA TASS fe %, 20°C a0 CoDpR rite HHH 1, TACT. 20: 602 F 444% Choi, UVBURASY bE a | | ereaersa avaneenes 8 8 shana) 126, CNMI (ABE ko AE He 2 pH Opa, C71) pact enc.a0t Shea oarPace aot eed septa0e spn 60 Spare 35-0 so peat Zasmmawese saan te) seacte (BL 7. FLARE 2 Hak o> a ie pH OfENge (71 BD 30°C-3768 (RAF (9) “CHL, FRIEO236nmf iE 292mm iFM RL I. x OIE PHS. BRET 4 & 2920mb}CHMEAMLUNLSSLO HL, pHs. EAUES % &292nmO WAHLER LT, 236mm SECT DUA Sivie, BIC, 30°C-602 HERA WOUVENLASZ bv SFR C27 (BNO). tL ORI 1EIMILE30'C CRA LE CNMB HERE BA OMTCAELL, MRO YER A Ate ‘Coven EME LTE01 2:35 8 7 OH 12 26 36 40 48 55 61 05 71 70 90 114 180218230271 30032037640 wom ra -®ACC, 200 es ACE. 107 eo" pH.200 ng heH. 20 ferns (2) (QS. CNMB fetta > SRA & pH ORRIEBEE, (20°C-602 FIA 30°C-602 FI) wm: ‘ACC:35ppm cer.) oar eae-rpn sic onoHD | ea pho sc smR0020¥0 Sst none) F210, 30°C-602 IMIDE Li CNMB Rett P AAG UV WAND 7 3) ALR Osea BUC, CNMBREREEDEEK (ACC:200ppm, pHS.7) EFUNRHERERRAE-K (ACC:17Ippm, pHS.4) 600m 4: 1 OTE. 7. 3-4 Pech, 10S E 30°C CHE LC EI Bt, GSD. pros, 20C-sn pK CIS ACC F58775%, pH83% EL, KARR O SIND abet: (BIN) SEC, PAIREEEOUV ELLA AF bVEMIE LLIB, WC AAR TIS, 236nm £ 292nm(= MEAD SAL (E12) . BHM eli TERR ( iE Hut. —I5, BOC 4ARFR TL, BonmHiTOMMITBonT, 292nmi ABRASIVE (3) 6 LAGOA 10 ‘om B00 8 cwacesoe 20 -eaccaoe 70 to BHO 50 OrPHIOT. eer 8K (ED BALL. CNMB if OB RIC A AOASEIRIE & pH ORNATE, 2D, IBIGR F-CIACNMB HERRERA ACO BREE 2.5 TW L. HMR AEM Ae NS Hee ce 6, 7223 ED (A00:176ppm, pis.) OMTBTECRReR EF Cit CEBR 2. 10°C-4 FIBRE Ate Le CNM AiR: PHA UV BIL ASZ bre ps {HLfE: 304nm, 330nm, 355nm, 300m SHOR GE:396nm BME: 380m MUEME An HT HCA Tx10Im B14. 20°C-391 BARA E CNMB eel PMLAROT SSR TE : c cues BD RE PLE AAG PRE CE MESA PAL PHO EET AAT AS, CMB SBVECNMB CHIN LLP ACIS, BITTE AMPOULE, RIMMED CEH, BIC, CNMB PARED, 30°C LL FORT PAR CLR ED PRERMNEL ELS. SOMME CORI AT EH EAT W, 20 + 391 FI £30°C-391 FAR EECNMB REED CATA, OF) ST VARESE LEE LS, fit ~400nm, G2C13100nm~30ONMAEIED J ST vas 10'mL S-CHHERBARTE (BIN, 15) FAILS, RL 3071 DCNMBFABEHERHTA (ACC:G6Oppm, pHS.6). I< FE, 200nm~SOOnmD I / 57 V%S.8% 1VimL AH L ie (BMG) , LLORAS, CMBa 3 1 SCNMBIL, Rey Ba RRR 7 STILE LCE BE 13, 30°C-4 FRIDGE Ltt CNM RHE EEA UV BIR ASZ bre LEE: 154m, 2520m,2770m, 3020 SEE: 23nm AH: Z7Bnm ERA 80m HRC Z9xt0IM B15, 30'C-391 APRYEL 7 CNMB Ant RIAD 7 URES $0 fE: 197m, 263nm, 445nm,482nm, SEI9INGE:9320m SSAA: 217m GGA: 1250m HF A: 5.8x10%m1 FHI 16. ME 30 47¥%o> CNM #OBRIECHTE A (ACC:60ppm, pHS.6) OF 1 <7 AEST EEA EILEEN LAME SIU, 1233, BAIA AT 7 ST rote OHARA TK OREN, 28 LMR 4 2 <7 Pe BAMEREDIIFIC AEM IV SAX 1327 9 7 SER$0 €E:72nm, 98nm,405nm, 1413nM SEEDOEE: Sdn "B88 2: 76nm RIGA: 16mm WH: 1. 3107 wi. (Colgarttitth, FE) 2486, 70nm~300nmD77 76 J de 1A ~ 13 X 10m. t> CMB Me We HE HE A (ACCSoppm, pHs) ABA (BHI) . ee 1) PSEA AeA BN : bow 3 RPA OMS OUT. HACIA A 2S A 2) SAUER, VEE UCR, PLB 13>: Dea BGK : BEAT, AER, ARB, Ute, AI, BE ANE LOMA. AREA, 80), 1-8, 2012. 3) FYPE10-24004: ROMER ARKO MEA 3, 1998, 4) #H2004.307405 : GHNRMEB AD BUEN HES UNG. 2004, 3 8 D 8) #8: 260nm, 289nm, 303m, 325m, SEDGE: 201nm REM: 285m, BiG: 250m FH: AAxt07IM 2 CATR 60 4) CMB HEREC (ACC:SOppm, pHS.S) DI STA VIER RDURI, BOACEEIE. “IERED (sz AASB BS SAR. AKA A 15, 508-520, 1994 BARAT HEE: iikogeetete: UE OR. AbiteAkwFF. 2,71-74, 2000, Issa-Zacharia A, Kamitani Y, Oshige A et af: Effect of Storage Conditions on Physicochemical Properties of Slightly Acidic Electolyzed Wate. ‘Their Consequences on in vivo inactivation of E.coli suspension. Japanese Society of Agricultural Technology Management, 15(3), 186-196, 2009. Nakagawara S, Goto T, Nara M et al: Spectroscopic characterization and the pH dependence of bactericidal activity of the aqueous chlorine solution. Analytical Sciences. 14 (4), 691-698, 1998. pHStabilization of hypochlorite solution by specific acidification ‘Tamotsu FURUMAT', Ryuji YOSHIDA‘, Toshiaki OHKT’, Kenji KIKUCHF, Linkai YU* and Yasuhiro IGARASHT’ "Toyama Council of Deep Sea Water, “Health Co, L., “The University of Shiga Prefecture and “Toyama Prefectural University Hypochlorous acid is effective against bacteria, viruses and fungi, although it is an unstable chlorine species. ‘Therefore, it is desirable to prepare hypochlorous acid water just before use. In order to sustain the available chlorine concentration (ACC) of hypochlorous acid water for long period, a breakthrough has been made by the acidification of hypochlorite with carbon dioxide microbubbles (CMB) or carbon dioxide and nitrogen gases microbublles (CNMB), resulting in the generation of stabilized hypochlorous acid aqueous solution containing CMB or CNMB. Interestingly, the CMB hypochlorous acid aqueous solutions in the PET bottles (closed and dark conditions) maintained over 70% of the initial recognized ACC at 10-40°C for 314 days. The remained ACs of CNMB hypochlorous acid aqueous solution under the closed and dark conditions for 602 days at 20°C and 30°C were 72.2% and 44.4%, respectively. ‘The remained ACCs of CNMB hypochlorous acid aqueous solution under the same storage conditions at SO°C for 71 days and 230days were 60.7% and 31.5%, respectively. In addition, the nano-bubbles ranging from 100nm to 400nm in size were detected at the level of 10”/mL in the CNMB hypochlorous acid aqueous solutions stored at 20°C or 30°C for 391 days.