National Veterinary Institute

(NVI)
ESAYAS GELAYE

17 – 18 November 2014, Addis Ababa

 Biotechnology:
Experiences and Potentials
 Outline
 NVI Introduction
 Vaccines production
 RD activities and facilities
 Opportunities
 Challenges
 Recommendation
 NVI was established in 1964GC (1956EC)
 Debre zeit - 45 km south of Addis Ababa

 Celebrated 50 years of anniversary

 The institute is Governed by the Board of Directors
 Working on cost-recovery base, with full financial
and administrative autonomy
 Certified with QMS ISO 9001:2008 since 2006
 Accredited with ISO/IEC 17025:2005
 Established Enterprise Resource Planning
 Host AU-PANVAC
 Definition:
 Biotechnology – the use of living organisms in
industrial processes particularly in agriculture, food
processing, and medicine. (Biotechnology, David P. Clark)

 Biotechnology – is the use of living systems and

organisms to develop or make useful products, or
"any technological application that uses biological
systems, living organisms or derivatives thereof, to
make or modify products or processes for specific use”.
(Wikipedia)
 PRODUCED VACCINES
oViral vaccines:
 Ruminants
- Rinderpest
- Peste des Petits Ruminants (PPR)
- Sheep & Goat Pox (SGP)
- Lumpy Skin Disease (LSD)
- Foot & Mouth Disease (FMD) – serotype O, A and SAT2
 Equines
- African Horse Sickness (AHS) – serotype 2, 4 and 9
 Camel
- Camel Pox (CMP)
 Poultry
- Newcastle [HB1, Lasota, Thermostable & Inactivated]
- Fowl pox
- Infectious Bursal Disease (Gumboro)
o Bacterial vaccines:
 Ruminants
- CCPP
- CBPP [T144 and T1SR]
- Anthrax
- Blackleg
- Bovine Pasteurellosis
- Ovine Pasteurellosis
• Poultry
- Fowl Typhoid

 Produced around 153x106 doses of vaccines in 2006 fiscal year

o Working to produce Marek’s and Rabies vaccine
 

NVI doesn’t produce Subunit/DNA/Recombinant

vector vaccines
ISO 9001:2008
 Fermenter and
Inactivator with
a capacity of
2,500 liters
Production Lab (under construction)
plan to implement GMP
 R & D Labs
Laboratory Facilities
 Bacteriology Lab
 Serology Lab
 Virology Lab
 Molecular Biology Lab
 Nutrition Analysis Lab
 Lyophilization (small capacity)
 Washing and Sterilization Room
 Serology Lab
 ELISA (FMD, CCPP, CBPP, AHS, IBD, etc)
 CFT (CCPP)
 HA (NCDV)
 HAI (NCD Ab)
 AGID (IBD)
 RBPT (Brucellosis)
 Accreditation for ISO/IEC 17025:2005 for four serology
tests. - 3-ABC ELISA for FMD
- C-ELISA for CBPP and CCPP
- RBPT for Brucella
 Plan to increase the scope by 20% each year
ISO/IEC 17025:2005
 Bacteriology Lab.
 Bacterial culture
 Bacterial isolation and identification
 Biochemical test
 Antimicrobial sensitivity test
 Lyophilization of isolates and storage in the repository
 Virology Lab.
o Animal Cell culture:
 primary cell: - Calf kidney
- Lamb kidney
- Chicken Embryo Fibroblast
 monolayer cell line: - VERO
- BHK21
o Incubation at 37ºC with 5% CO2
o Cell storage using Liquid Nitrogen
o Regular checking of cell viability and sterility
Cont…
o Pathological samples processing using Biosafety C. II
o Virus isolation using susceptible cell culture
o Observation of CPE using Inverted microscope
o Preserve isolates using ultra freezer
 Molecular Biology Lab.
 Nucleic acid extraction (DNA/RNA)
 Conventional PCR
 RT-PCR
 Gel-electrophoresis
 real time PCR
 Purification of PCR product
 Quantification of PCR product (NanoDrop)
 Preparation of PCR products and
 Sequencing of gene/gene fragment
Cont…
 Sequence analysis (Bioinformatics)
o editing and contig formation/Vector NTI
o blastn and protein translation/NCBI & ExPASy
o multiple alignment/BioEdit
o phylogenetic tree construction/MEGA5.1

PLoS ONE, 2013, 8 (10): e75971

 Opportunities
 Good working environment
 Better Lab facilities
 Experienced and trained experts
 Strong research collaboration with National and
International research institutes
 Lab animals availability
 Existence of animal experiment units

 Product Development (PD) Lab - established by

NVI, PANVAC and GALVMed.
 Lab
animals
 Research collaboration
o IAEA - TCP and CRP, AUSTRIA
o FAO/IAEA, AUSTRIA
o CIRAD, FRANCE
International
o GALVMed, UK
o Pirbright, UK
o AU/PANVAC

 NAHDIC
 EIAR (NARF and ARTP)
National
 Universities
 Regional Vet. Diagnostic Labs
 Achievements
 Production of vaccines and diagnostic kits
 Improved capacity of doing applied research
 Improvement of existing vaccines
 Development of new vaccines
 Increased collaboration on research projects
 Increased capacity of expertise and infrastructure

 Future plan to be performed by 2015/16:

 Cloning facilities
 Proteomics
 Bioinformatics center
Contributed for the eradication of
Rinderpest (using Thermostable vaccine)
 Challenges/Constraints
 Research fund limitations
 Shortage of major high tech lab equipments
 Limitation of consumables and reagents
 Capacity building – training on specific technique
 Tax problem

Recommendations
 Capacity building – training and Lab facilities
 Allocate Research fund
 Organize workshop
 Evaluate Biotech course curriculum
“Committed in producing and
supplying quality veterinary
vaccines”
“ Thereby contributing for
agricultural productivity and
food security”
Environment-friendly Inst.
Thank U All
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