= “Sock py Fayre lad reas hae Oy wy pene we IST@-LOGIC Reiter, Leet Luma, B, Lit. HE (ASCP) reese letin for Histocechnology Published: January, April, duly. Getobier ‘Vol, XI, No. &- July, 1981 Garbol-Xylene (A Useful Too!) ‘An Editorial Carbol* xylene was a very useful too” in the histopatholo- gy laboratory some years ayo, primarily duo to ite softening tifecton tistuo avetions embecdod in colloidin. Sections ob tained from celloida-embedded specimens had a tendeney to harden when expoced to xylene, resulting in séetion curling, ‘This often ‘wrinkles und, of course, distortion of tis sue section. Carbel-rylene was ised extensively during the celloidln era to poften these sections and allow them to un- ‘curl and lie at against the aide proot tothe coverslip moant- Ing. The strong emphasis on the use of paraffin over the last fifteon years ‘has relegated carbal-ryiene to the disuse ‘status. However, this station exn stil be very useful on or. ‘easion when one is experionting dotachmont of sections from fldova. This i very used solution for tives such as bona, chitin, or estions coms! Ee tea mepanis meer okie nek meee te dislodge from tho glass slide. Solu Cachet: Xylene Phanol(earbolie seit : 25.0 gm Xylene 1.0m ‘Boe rocedary I empl fo wie by smpleying the fleying {UR eae lr brash pd nth sien of tral mies ‘brush is then applied to the fulded portion of the speel- Poe (tea beet ol cata ngten pee a tees gad oes ey unfolcag by gentle touch rus §. Remove excess carbal-sylene trons section (If casbal- xylene is left on tha section, tinsue will fade] 4. Mount coverallp with a resinous media. "Phenol ar carboie ac £. Liver Trichrome Stain William Dotson ‘The North Carolina Memorial Howpital Chapel Hill, Nerth Carolina 27514 ‘The “liver trichrome” procedure is one ofa nitery of stains we'do om tll our Iver baprias The pathologies seem es ike i beter than ths Manin ihe, fis cfr harper color contrast. tis being presented here in thers will ind benefice, Fixation 1055 buffered formalin or Hell's satution Microtomy: (Cut parafiin sections at 2 microns ar desited thiehisess. 70Rm + 100.0 mL Alcohalle Piero cid (stack), 2 parte Bthanol, 05% part 5% Aniline Blue Aniline Blue 50—m, ‘Aectle uel - 25! Distilled water 10000 mh 1%. Poacenu de X3lidine Ponce 28) Ponceau de xylidise (Harleo} 10 gm ‘Acetic acid = 10 'm Distilled water 5 1000 ml if Neate Aela Aciti ued 10 mt Distilled water 1000 mh 18, Phorpoimolpile Ae Phosphomoly bie acid. 10 gm Distilled water B 10000 ma io slides and hydrate to distilled water, Harvie" haratozylin i 85°C for minutes, ‘95% ethanol. ‘working sleohotlc plerié weld for 10 65, Wath alides in ronning tap water for 10 minutes. 6, Stain slides in ponceau de xylidine for 5 minutes. Treat slides with 1% phosphomalyblc atid for 5 mlnutes {uae aolution only oneal. 8, Stain slides in 6% aniline blue for 2 minutes, Rinse sides quickly in tp water toremeve excel 10,Tyeat slides with 1% phosphomolybdic neid for 5 minutes, L1, Place slides in 15 acetic acid for § minutos, 12 Dehydenta, clear and mount caverslip. with resinous ‘mounting modin Results Connective tismue and collagen — koe ‘Muscle and eytopinam = red ‘Nucla le Making a Desk-Top Out of a Drawer gay art More US" Army Biomedien! Labor ‘Aberdeen Proving Ground, Maryland 21010 1 quently vane ob alo rv a ota seontog ey eg ecesge booster tee oe Aue cao mea ab edranetacer sre: ut top. Tt isnot difficult to make such 2 utility out of any drawer 163aera cnn ap, epi ho moan. xin ‘Stor nom penal se een hose cabinets the drawer: ‘wolls ar considerably larger Um the drawer : FIGURE 1, a igi rd ht | yk sd wie “draw, isobtained oot upon theright and att dear (Pigure I} A board at leant three qunrters a3 deep (fent-to-bockl na the Graver provide an adequate working _bavacs lor eeny purposes ineuding woe mk stnad for Shatstewon scroncop, On te undead of the bout tw mllrubbes ect are fastened (ny news or tack) ina Cee be tea tho right Ie siden aa gurces Figure 2 The ber guides arepoatzned st. dctane rom the eds of the board to harly touch the sides ofthe crawer with the board In plac, thie assuring a free back-and-forth more. thot without sidenway’ The distanco the beard tp ean bo Posted back, and Uo aren of inner cawerespac a son the ovation ofthe guide atthe seat of te Hyatt pcan Oe aes Gere an ne tea eee FHrke the bok edo of te arawer. “To determine the proper Ineation ofthe 2 gear es, the “bards dom ne gaan itt ide of vb des with ts tack edge eaielding withthe edge of the back side ef the drawer. ti rth thr board in ths positon, necons the daar is adequate, the rea guides are attached agaast the tear edge on tht undorvde of the board: I, hemevan ir openings detec (ha ont guldes ie aceure ie Sardar a stance fra the reared equal othe nl aa eulnctac ct opesiag tarsi FIGURE2, 164 “a handy pl for manipulating the drae-op can ba made froma length of ap, Contact paper ray proce smoother ‘more attractive appearance. Ina enbince where ls too small to allow the entrance of « bond ‘atop the diuwor slides, tho board must. be romoved for reaching aud looking into, and for closing, the draw ‘Note:The authors are thankful tr, fy Clawon for the photon: Bi ele Muscle Quenching with Liquid Nitrogen and Talcum Powder [Leonard Noble and Verikata Challe, M.D. ‘North Carolina Baptist Hospitals ‘Winston-Salem, North Caralinn 27108 To achieve enzyme localization and 1o eliminate ice-erystal artifacts In musele blopaies, the use of liquid hitrogen os a coolant is prescribed. Isopentane and various fluordearhons can be caoled down by liquid nitrogen to a temperature ‘which will adequately quench muscle blopeles, However, the Sere tcl pee ee rie ening aoe cen since sed dcteicotssepeaac Nae oa We feel that the method alebalcet we ibe guid allvoeee Tks osever es aemnpiah lg recuniig ant fees Teper cl pacnd ein ec nineaa ecnee “thrust into liquid nitrogen. This layer of gas acts ax an Sette ee rere tic etc en Suen toute os oetae upto wesiee ee haiieaig | tanriass to taetia Gun at cen cose falls away from the spocimen, the gas layer is «eliminated, Tes tloaty Coat tessa pares e eleongerece Gr at wih ee eed ttn th Sane et grea ‘Moline and Glanner! reported the peed Deselect oa eee nee Fla faperivetee ants noone vee tec naa ‘of tissue is possible using liquid nitrogen oe and not in aerate neat tata ar __ When adapted to murela biopsies routinoly, cis t ca is safe and relatively inexpensiva, Freeaing can actually: ‘zea adjacent to the biepry room, thus aveiding 2 with saline, Alter quenching. the specimen re- Sas uur meet arin ult Wu mesetiod te ieee Meine Nan ote: ‘Liquid nitrogen: OCT embedding compound® cers = ‘Cork dises (2.4 em in clrcumforence x 04cm in thickness) ‘Dewar flask - 1 liter capacity Soliton "rotmba ir eral ooat OCT eabadng ompouns ns wal tin of OCF abc Te cet ee banger tained. Discard any leftover paste a(tor frooring specimen. “Menoa: For best results, the blopay she sess 7 min. long and no fore than mtn oes Et If ty dinesions on eng Innger,subanguent tring may be ners. Eve ae cd fe ih tote amit of the taleur powder OCT paste, ‘the biopey in the paste in such.n way arto beats clo fibers whan sectioning. 2 ‘Coe tbe the entire surface of the cei sore 13 pn ths cok ies with the tala porter5 = National Society for Histotechnology Symposium/Convention November 16-20, 1981 The Seventh Anal Sy mposiym/Convention of the National eee ali wi bo cic tate To tipi elagita or al pa ha form i catent vil tii 40 coping rove a the ‘Lith America, with ovrfiow aecommedadinn in the > Are fta oubat-rs (rch il nen cmd eel wotaton ad Ue Ton Ian, 80s saath hotels are ‘eithina hall block of the Little Ainericw and) utanet All rots etervatind wl ifrugh etiti Amerc ihe a wal lb orwarded to olher hots for BUEASE MARE Vout "nrsenvarions WCE ALL ROOMS BLOCKED FOR NSA WILL HE RELEASED ONE MONTH PRIOR TO MEETING DATE. ‘all Hotel Reaervstinn Dirvetly tr Tite Amarie 600 = sini eae etfor ih tsa. rt an may be pho acpi aon ng be sae ey ee "0 etic HER stp od held abeyance Oni al commie Ze fe = lease include nota to ths efector you estes thon form To ald « LATE REGISTRATION CHARGE, be xo ‘tention | i teal aad ale liclss™ at he vesting wl bo HEIMURGEAIENT of sagitation tes il be ae nn notification prior Ya, Nove ie NO TRENDS ILE BE Mal itn E APTER THIS is for unattended wor ‘or bane "orkshopt esioas me nko, WHLL NOT br mis heres cota nse a ee tl = ing wl not be sade when changing Workshup altar dance alter asia tthe mostin. Fe elon een, poae call Ratrta Mosedale, NSH Office, 301/602-9678 MATL. registration November 16 20,1981 _peaminglons daring Salt Lake City, Utah and check to: NSH, P.0. BOX 88, LANHAM, MARYLAND NSH/Thomas Edison Program Schedule ‘COUNSE REVIEWS: Shadents interested inreview estos kd enroll In one of the workshops scheduled Monday, Werksien expences a torcrntilaats Gena aol (g “Thomns’‘Edioon College. STUDENT Mi TROOP OF ENROLLMENT WITH REGISTRATION FORM, ‘Twesday roviow sessions are only for thove planning to take din te cope won, doa wl ae fers canine Co Hanah ect reve mea ree EE amines are encouraged to attend both seasians INTRODUCTORY HISTOTECHNOLOGY!AISTOCHEN. | eo J ‘itlchard Schroeder: HT (ASCP Monday - see Workshop #3. ‘Tuesday review, Nav, 17; Pawell Raom 9AM - Noon HUMAN MICROSCOPIC ANATOMY hide Crnon, PhD ‘Tucodayseriew, Nov Te Hayden oom 9 AM - Noon SURKENE CONCEPTS IN DIAGNOSTIC HISTOPA: Gite El Ble, MAG Manda aia Woreanp ‘Tena review: Now 42" Pxcalante Roos 8 AM - Noon EXAMINATION SCHEDU! Wedoowlay, Thursday & ilisy, 79 AM, BRIDGER NATIONAL SOCIETY OF HISTOTECHNOLOGY fi ROOM RESERVATION REQUEST \ wate mes ADDAESS 1 3 LITTLE ery are = ; AMERICA crouse = é = TRADITION or CER Lr aera Rion i onl emis ea Bt Aes | sie Ch ret ard ha Ce i Neseronthe mat be rmloo 2 ween i eet oe Spas ACCOMMODATIONS Semi ceee th ec ati owe | SINGLE OCCUPANCY 0 8 | DOUBLE OCCUPANCY. 5H mn Boe ‘vin ocquraNcr = ta Teena} Employee: ae (eit Work Telephone No. ie $ Peter Home a ‘This a your fist attendanee ton NSIT Symposum'Conveetion, Yer “Arw'you an NSH Meanber? Yer No___ lente check functions you dai to ltond and complete Reistration. “ygeaneesi rit nm eS See ee ‘MAIL empl tem et. cd aE Eeasrw sin sorii etnscmsachiteea cs era finan oo DONOT USE THIS SPACE LL Site acnaaen ewer Basa eaten ee eaeee et ‘pts Reon aut ct a ee aa i are ere teen Se a etoetaanige tems Ge eta ae ee eerie aiee meat rice ete Soe eaitamen cused aes SESE SERSetter Tuesday, November 17, 1983 eid cel sale ad Loe Seana biomes DeRecdome igen aire dat ite Ft fe ape i Meret eet Spent Site i eneeee hrm emection ei ioe, PEE treme nme Tintern AM Hite ‘ ri hs ne ih arn Thadeeing ee ce eh ee Bbteaee americas ret enraternis Renta! Feral pa Ee oping peg aera eee ee oe omneeonn aes {ieee icine nae tine aed Arta ea neon Meese praetor dare miele tel te! Lyre. ia wy eee ia ta tr | ES ee ei ae i iy i me E u ai F a i i i EEE ui i Ls E ral i He : oH a i He i aEETE, i al eestr ni a a i i : : ll i Hy i f Hl : ea SSI ih ie eee ct ate te a ge career eerie diene tea tees Beda ceca ati see Shieelemetan eenmeee ; Peer eee eee ae ce jer Api ares Raee sree tase teens eet ee yee lle: Marta Carries be Mah snd eae, Misr rea + et nen on See eee eee ea eat Eine rps See eeaenes Seen eee tif i L i rea Ge aetrir eete ae OBS eter oer : Steen ie . ea pita Ei Tea pvey mma i = Fennena eres arate, | Sats Malignant Lymphoma, Recent Advances Open Lung Biopsy Forensic Histopatholoxy ‘Lung Cancer — Histology Correlation with Response to Troatmant ‘Moratity Study of istlogie chniciaas Covllad Walween 164-1070 ‘Routine and Special Stains for the Diagnosis af Liver Dinenoe Estrogen Heceptor Analysis; An Immunofluorescent Method Hormonal Evaluation ofthe Cytologic Sample 7-9 P.M: “Simplifying/Enjoying Parlimentary Procelare ‘Soasion will ave mba introduction tothe basics of parllamentary procedure, ‘There will be mock ses sions using different situations that illustrate how to sc- complish varying results starting at the same place, Finally there will be par- Uamentary “gamesmanship,” ‘Thareday. November 19, 1081 AM. Session ‘Testing for Evidence of spe Hintochemistry and Sports Medicine ‘The Pathology of Tumors P.M, Session ‘Aleohal — The Cheaseal Drug We Delak one Marrow Preperations and Interpretations ‘Toxicology — Drug Testing Immunoperoxidase (PAP) Techniques Carl Kyeldsbers, MD. Jerrold Abraham, M.D, Wadlace Graham, 4D. | Harmen Eyre, MD. “Dan Graumon Randy Lee, MD. ‘M. Blieabeth Hammond, MD. Catherine Keebler, CT (ASCP) CPLAC Lola Cob, BS, HT (ASCP) T: Paulette Sutton, MT (ASCP Petar Senzig, HT ASCP) Robert Flinner, MD. Richard Schroeder, M.A, ‘Gatherina Branse, HT, (ASCP), Maridyn drwin, HE VASCP) and ‘Kathy Daois, HPL (ASCP) Thoma Jennison, PRD. ‘Joe Morty, MS Friday, November 20, 1981 AM. Stision: ‘The Interpretation and Application of Diagnostic Special Stains for Surgical Pathology ‘Animals with a Total Artificin! Heart: Tilood and ‘Tinaue Interactions ‘Toxie Shock Syndrome é 5 Histologic ‘Technique: Skin Soctioning for the Dermalpathologist Lecture from Paper Written During Workshop an Tuesday Brwia Haws, HY ASCP) Donel Ol, D.V.ME Allen Paris MED. y Diane Milter HT ASCP)3.Quickly immerye the cork, with tismue down, into tho Higuld nitrogen. 4. The frozen specimen is then left in the liquid nitrogen for ableast one minute, 5,'The cork with frozen specimen attached is then trans: ferred to the eryoutal 6. Wait for u period of 18 to 30 sccoads before attaching the cork to.n eryostat chuck with OCT ershedding compaund, 7, Allow the temperature of Uht frozen spotinvin to warm to ‘the temperature ofthe cryostat. Sectioning can naw begin, Rafarancs: E Mole, SW, and Gleaner, 6. Uitrarapid Tiseuy Pressing in Liquid Nitrogen. J. Histochem, Cytochem, 12.777-183, 1964 OCT embedding compound inn product of LAB-TEK DIVISION, Mies laboretren nc, A0W¥eH0 North Aurora Nou, Naperois, 60588, ‘Pre-cutcork tues are available from Sle Inlarnationl ne, New York, NY 100t1 Replies to Inquiry Eater's Note: Tho following replies were received in rogard to shy plastic conical tip eentcifuge tubes produce more cempact buttans than pase conieal tip tubes. The original ar tick, authored by Brenda Cuevas, appeared in Histo Logi, Vol. X. No. 2. pg- 148, April 1080, Priscila Ane Gregory (Osteopathic General Howpltal 1750 NAB. 167th Street [North Mists! Beach, Florin $3162 ‘In respanse to the article by Brenda Cuevas, following is our procedure for Call lod ion, Cell Block Preparation: Plasma - Thrombtn Technt 1, Gentriftige the fluid sample st 1600 to 2000 rpm for 16 a Dent |. Decant supernatant, 8, Add 3 to 6 drops of plarma (plasraa with normal PT time), 4 Gently ati with wooden applicator stick ta permit plasma Wo putmeate tho sediment "5, Add3-5 drops of throinbia (Ortho Braln ‘thromboplastin. ‘6. Gently sts with wooden applicator stick to allow thrombin ‘to form w clot. 17. After clo feetns, place in lens paper and place in embed ‘ding cassette, 8 lace In formalin for tissue proceniny. ference: Pathology Amoual, Part L, Vol 12, University of Marl Medient Cantar 1077. George W.. Chang ‘Unlveraity of California College of Natural Resources ‘Berkeley, California 04720 ‘was Interested to road Ms. Brenda Cuevas" note about “Call Blocks from Specimons of Body Fluids," suspect har . observation has something to do with the fect that water ‘doest't really wot the plastic cubes. Thus, there i less waear ‘remaining at tho bottom of the tube after she pours off the supernatant, The smaller amount of water (uid) remaining ‘ay’ be lees likely to resuspend the compact button of cells. Section Adhesive for Paraffin Sections Alice Expouito Methodist Hospital Philadelphis, Pennsylvania 19145 ‘The flowing progtie ff athsn of un ste te ‘nlereseopie ali foun o be very beneficial inowr fbands, Some of the benefits nrc () reduced insu tection dleinchment trom glass slide (2) improved staining qual (Gi reduced reside on rleroscopie slide; (4 less writen dant on finished lide; bed (6h saved te, Procedase: The Hse section ribbon which a oblalned frm milcrotomy ‘in placed in the flotation bath coatalaig the following solu tom The waterbath orsprnti shall sy nua Ee Adhesive Solution Alcohol ethyl or isopropyl) 1000 mi Distilled water 0... 2400.0 ml Elmer's Glue 6.0 drops Mix wall Seclion are picked up.on glass sides and placed ina staln- jog rack which's then placed inwalide dryer at 65 for Ls Malning: Nove Flotation bath aad ade ver obese ote: Flotation bath an temperatures tre most Important when daing this section edhe, The hase anton should ba added ta water be ort prior to ‘*Can be prehumd at moat drugstores and food star, Problems Related to Staining of Connective Tissue Embedded in Water-Soluble Plastics Peter 0. Gerrits nt Ane tureay eceenrs om The Neherancs 1n cur laboratory we routinely prepare embeds mca on the basis of watersoluble methaerylates JB, GAUA, accord. ng 0 Hel! 196- and Sims! 194). The iiowng prob ‘Although most staining procedures we use in routine his- “tology do not aller any rignsfcant problems occasionally ‘nly alight modifications appar to be necessary wo a7e Te ees eee rae ‘ones tnnue eomponeal est srvtors do not mention similar dffcltien while toons Keow- ‘tge, un effeetivo connective tinny staining proche For Plastic-eabedided spctrnane haa not been 1 ‘contrasts between muclei and cytoplasin in relation to other. fiasoen T would gratefully appreciate any sugentions om this matter, Edtars Noic: Please forward a copy of replies to the [Baitor, and to Mr. Gerrita ut the address above, References: 1 HoddlC1.: Eetadsing Media fo 12 Mleron Secning: Hy Genetny! Methaeryint Comtlnva wis tbutanjetane aa 1. Sta Slap Mle of gta 12 Mien Sen of Large Titus Blocks Using Geil Methncrylate J Micese (Geih ioices-ae, 100 166Hoppe 8 UIT AONE Y DIDOT-OLSIH a oH ey 08 410d ava apvisol sa, “uve xa Write on ease) wopoeueg seb ‘ovs00 11 ‘aunaden “py mioitiy WON, SzPMOE ‘u} 'Sapojev09e? SONIA NoIsInia xaL'av1 Oz # aE gt ats ee ars | ie ie es 2 He fi ams 2 Sawn ey ar a ore Ped = ae aE Re and on and on... Tissue-Tek III Cassette System™ for accurate, time-saving specimen identification. The Miolauy system wurce Can You Help? =~ tse Mob Bosman Linboratorium voor de Volkewezoudh eld elsumerstrant 6, afd. P.A. ‘SOIT EN Leeuwarden ‘The Netherlands [have a probes in determining and caloring spirochaete ‘The mathods used are Levndit'sroethod and the Warts Starry inithod. Who can help me to positlvely identity (1) ‘Freponetna Baliga ype and (2) Leptospira Ltrohter tirhagine (or Wall's disave) Inver tissue or lympbo-qland {issue tte in formalin, Should there bo other teh {Ley Tvrescence methods) for determining of spirochaete, 1 ‘wouie ike ta be informed. Tutors Note: Plase forwacd « copy of any replies to tho ‘Réltar, and Co Me. Hob Borrue at the addres above, “Ty yresne your gn preresi copy of ISTO: LOGIC, to hoe oh nalts HAP malin vel hone woes, Lay Te Din Miles ‘Ettore, ne, aDW48 North Aurore Nayar, in 3540, Titan USA 66 ‘ed tnmerting togeimen 1D. numbors with enprales ane ist Now you Camis ee tne Ler grossing tig, as {ine seine erate ae Vour Bement seinen Secor These eases hive 3 ih estan te solve nd they are gompae wth ctw itch and ange lock owide peste specimen feventon and ¢ fisotable td means no mare Mata COW 0 Clean Alt designed To ake your la Wf erer from LAB TE. Call our LAB-THX elatrovtor day about the convenient The Tek Ih asset Sytem which icles Unt-catettett -Capcte i Four Colors for ber cxyanitation ‘lonuy Uncoetel™ with Trum pores Hale tmnt the rod to win rat bien speek s htega Catt for nrg specien, such a hoot, wy and TRE Spec ngs Castetie base alka avaible ‘An Atlas of Tissue Artifacts ‘Thiy is the only semmprehensive book on tissue artifacts tallable te Ristopathology technicians anc pathologist Contelns rors than 400 photusraphs Sustating anny Ceo mon wd uncasaron artllets produced in all facets of Msto- {eehnology, More important contains detaled written ka- formation on how these atlfacts are ident produced, prevented andor eliminated. This book isthe result of more {han 20 years of studying tssoe artifacts. 1s authored by Samuel Wesley Thompson, D.V.AL, M.S, and Lae G. Luna HT (ASCP). The book is availble from: GhaslesC. Thomas, 301-327 E, Lawrence Avenue, Springfield Minois 62712, “Ti ioe wine Lo ple atria, quai, ad cen ei to ‘itary Lae , Lam ator, Fon Logic BO Hot ‘tenho Monpssd 29006 Arie shotgrapo, allt borer hie rues i ig ten ty wre er,