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RFLP

Related Concepts (6)

RFLP
Genetics
Cell Biology Restriction fragment length polymorphisms, or RFLPs, are differences among individuals
Scientific in the lengths of DNA fragments cut by enzymes. Restriction enzymes are proteins that cut
Communication
Career Planning
DNA at short, specific sequences called restriction sites. After a segment of DNA has been
cut into pieces with restriction enzymes, researchers can examine the fragments using a
laboratory method called gel electrophoresis, which separates DNA fragments according to
their size. If two individuals have differences in their DNA sequences at particular
restriction sites, then the restriction enzymes will cut their DNA into fragments of different
lengths. There may also be differences in the number of DNA fragments observed among
two or more individuals. RFLP analysis can be used as a form of genetic testing to observe
whether an individual carries a mutant gene for a disease that runs in his or her family.

Further Exploration
Concept Links for further exploration
restriction mapping | restriction endonuclease | mapping | gel electrophoresis | gene |
genome

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