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in suitable solvent.
Take some tablets and crush them in
powdered form.
Weigh 100 mg of paracetamol into
100ml of volumetric flask then add 15
ml of methanol and shake well to
dissolve it.
Add 85 ml of water to adjust the
volume up to 100 ml.
Draw 1 ml of solution and put in 100
ml volumetric flask.
Adjust the volume with diluent up to
100 ml.
In single beam UV spectrometer that
has only one cuvette compartment,
place the methanol, which is our base
in the cuvette.
Go to absorption spectra by pressing
2.
Press zero for baseline correction.
Now add the sample in the 3/4th of
the cuvette.
Press start to obtain spectrum.
Press F2 to check the maximum
absorption.
Make several standards of the sample
that is change in concentrations and
name them 0, 1, 2, 3 ,4 and 5 and
compare the maximum absorptions.