1292 PRINCIPLES OF PHYSICAL CHASTRY From Eq. 77, Ry can be exsily determined, The value of Ry obtained as above is incorporated in the Zimm equation, viz. Ke «1 fy y2pe+(2)s292 He ftean (Sa) 5 and the quantity Kc/Rp is ploted against (sin? @/2 + Ke). Here K is the seattering constant which depends upon the refractive index of the sotutioa; sin? (@/2) is proportional to s*. The constant is s0 hosen 5 10 make the data pins spend conveniently over th graph. The experimental igh catering data ae pled over wite rage of conentation ¢ and angles 0. Lites are drwn through pons 2 constant angle and extrapolated to 2 concentration Alo ines re dewa ough piss at constant concenaton and exraposedw 20 angle. The two es of lining values ae then extrapolated to common intreet a eo valle ofthe abscissa from whece we get the ale of 1 Tae by of grapia plot obtained is eed the ium pot. typist Zim plot forte iht scattering by collagen at 15°C i shown a ig. 2. The molar mas, iy, determined tom the plot is 310,000 g mor" a] ea of Key aps "fenton Fox seer He OL Seey | Rez of al exwapltion 3 Taesant e109 =0 wioveresoseaoTeaon eT TITS TTSTOIT (2) +1 0006 —~ ig. 2, Zim pl allagen at 15°C Ik may be mentioned once again that ight scattering gives mass-average molar mas, te teason being that the intensity of light scatering is greater for larger particles so that the average is weighted nore heavily in their favour. ‘Turbidity. The searing from polymer soltions can also help in the determination of a quantity called turbidity of the solution. The method is snilar 1o that of measuring the absorbance of a Solution in a Beer's law cell, The imensiy-T ofthe scatered light is measured after itis passed ‘through a dilute solution of te polymer, Mathematically, T= het A) where pi the intensity of the incident light, x isthe path length and ¢ is the erbi [i can be shown theoretically tat the turbitiy is given by + =(68)akMive (80) where K isthe optical constant defined by Eq, 73 and c is the concentration ofthe polymer in rms of ‘mass per unit volume, Le, ¢ = nliy/V - (Nowe that ¢ is nov molar concentration bere), Aso, from Ea. 71, Ry =Kitue “ MACROMOLECULES e Hence, ae = += 060% Thus, fom + measurement of, we can alent, o, facat calla, Since the size of the plmer parle may Fe sapere ne a feng ogi wean we a viral expreion ote pes SOPHIE Oat ofthe wae £ ~(0 + 2BE+ (8/4)22 + on) @ ete B, Cae the second and tiv vical conics, hi vi is, respectively. The valve of 4 fiction of ¢ and cit is plated a 2 futon of The iret at CoO res mae aS en The intercept at =0 yields, vide Bq, 8 he (2) aber “0 The apparatus for wridy messfemens is valle commercially DIFFUSION i fifsion of pares through & solution isan ipgortntphesomeaon:", is ck zero, Ithas bees shown that + etott = apne 9) Eq. is known ate Eiisten-Smobichowsi equation, se 10, ‘Te difson coTcient of myopia fa water at 25°C is Q-1LDCI0 mw 5% IF the i erate ef cP ey cetipuse,calcalate the radius of the & cofiieat of ists of water at imyolbie mola Salaior 1 ie, Poie)= OF by at fe Leh = DelO?P = PIO? by ott Since fom Ba, 93, Ds HIG) bce fa (138 10° 1-098 K) 8 Feb” SEATON AO gw TOTES x10 = 91x10 9 = 191 om = 1,910 pm Oe tgat st rample 11, Cale the ine rue fr gaa meat die though a tance of 1.080 A sie Bc naan a cee OD a Solon : x = 10.000 A = (a0 A) (x10 wi A) = 110 According 10 te Fain-Smoluchnst etn (E90 <>! = Dg tt

= Ir x04) 0-462 0S ge vanci0’s ees 3B | | | Macrowouecuies rome 12. The ates cet AE 1: The itso cfc sbmaane bs HI0S ent sn 2° € Salton: Dur pe AL £3810 HY STKY Do Ts a = 30004 ate sexi Emile 3. The deen coca tert conus at nae mgt. The diftsion coeticet of inst 25°C is 1510-0 pea O01 Fs 5 eine te rads te IU te acto vsty Sins” "Lt o1 hs motigat et * 000m = Oa tw From Ea 93, 3 a oe reg 1-38 x 191298) 8 TR STS = 1682x107 m = ™ set = 1 ce sng Rie MA, ed of ge 48 pens gS se aS SS SE ee Soation¢ saa nae a 18hgee = 1S. wt 1 = 8937x10°R = a957% mist ‘Hence, from the Stokes-Finstein equation (Eq. 93), wane it 7 (38210 sgn Se TT a SATA = F510 Faget ort gt = 989 * _ ‘Vohime of the glucose miolecule is given by mere ° tented V = GHP = (43(01959110° wy = 194 2° a? m= Vb = (54x10 m (55x10 bg a . 01x10 ig = F01x102 ee, the molar muss of gcse i gen by . M = te = x0 a} 1 cnc tay ig te aw a) ODIO p= 0 that its mas ie gien by sate Sey mh etional ratio is close to unity, ‘than unity, the molecule sat knowledg ori eer ‘eral spe ts vine se ascertaining the shape of biomolecules. some A _ faa fra co BOM, $120, tint, a is et rs tn ete we a Ppa ate omen ae Sem ra oe ‘Since from Eq. 93, D = kif ine AE Os . 6-910 mgt