0ral histology

Lec-1
Prof.Dr.Raja Al-Jubouri
0ral histology:

Is the science that study teeth & it's

Supporting tissues in the oral cavity.
This requires preparation of very thin, high quality
sections (slices) mounted on glass slides &
appropriately stained to demonstrate normal &
abnormal structures.
 Types of microscopies
1- Light microscope: by using visible light &
a system of lenses to magnify images of small
samples.
2- Electron microscope: include
A-Scanning Electron microscope.
B- Transmission Electron microscope.
3- Fluorescent microscope.
4-Confocal laser scanning
microscope and autoradiography techniques
Slides preparation
1-Fixing : to prevent postmortem decay
(autolysis & putrefaction).
2-Processing.
3-Staining.
Aim of fixation
1.To prevent autolysis & bacterial attack.
2. To fix tissues so they will not change their
volume & shape during processing.
3. To prepare tissue & leave it in a condition
to allow clear staining of section.
4. To leave tissue as close as their living
state as possible, & no small molecules
should be lost.
:Factors affects fixation
-PH.
-Temperature.
-Penetration of fixative.
-Volume of tissue.
:Types of fixative
Acetic acid
Formaldehyde
Ethanol
Glutaraldehyde
Methanol
Picric acid
Processing-2
Stages of processing:
A. Dehydration.
B. Clearing.
C. Embedding.
D. Sectioning.
E. Staining.
Types of dehydrating agents:

Ethanol
Methanol
Acetone
Staining
H & E ( Hematoxylin & Eosin):
Hematoxylin (a basic dye)-stains nuclei
blue.
Eosin( an acidic dye)-stains cytoplasm
pink.
Types of sections
1-Ground section (non-decalcified tooth).
2-Decalcified section( soft tissue as pulp,P.D.L
3-Frozen sections.
Thanks for Listening