Professional Documents
Culture Documents
1. In the prosecution of the criminal offense, the identity of the offender and that of the victim
must be established, otherwise it will be a ground for the dismissal of the charge or acquital of
the accused.
2. The identification of a person missing or presumed dead will facilitate settlement of the estate,
retirement, insurance and other social benefits. It vests on the heirs the right over the properties
of the identified person.
If identity cannot be established, then the law on presumption of death (Art. 390, Civil Code)
must be applied which requires the lapse of seven years before a person can be presumed dead.
In special instances, the seven years period may be reduced to four years (Art. 391, Civil Code).
3. Identification resolves the anxiety of the next-of-kin, other relatives and friends as to the
whereabouts of a missing person or victim of calamity or criminal act.
4. Identification may be needed in some transactions, like cashing of check, entering a premise,
delivery of parcels or registered mail in post office, sale of property, release of dead bodies to
relatives, parties to a contract, etc.
1. The greater the number of points of similarities and dissimilarities of two persons compared,
the greater is the probability for the conclusion to be correct. This is known as the TJIW of
Multiplicity of Evidence in Identification.
2. The value of the different points of identification varies in the formulation of conclusion. In a
fresh cadaver, if the fingerprints on file are the same as those recovered from the crime scene,
they will positively establish the identity of the person while bodily marks, like moles, scars,
complexion, shape of nose, etc. are merely corroborative. Visual recognition by relative or
friends may be of lesser value as compared with fingerprints or dental comparison.
3. The longer the interval between the death and the examination of the remains for purposes of
identification, the greater is the need for experts in establishing identity. The process of taking
fingerprints and its examination under a magnifying lens requires the services of an expert.
When putrefaction has set in, the external bodily marks useful in identification might be
destroyed so that it is necessary to resort to an anatomical or a structural examination of the body
which requires knowledge of medicine' and dentistry.
4. Inasmuch as the object to be identified is highly perishable, it is necessary for the team to act
in the shortest possible time specially in cases of mass disaster.
5. There is no rigid rule to be observed in the procedure of identification of persons.
Methods of Identification:
IDENTIFICATION OF PERSONS
2 Those which are based on scientific knowledge — Identification is made by trained men, well-
<
In the normal process of walking the rear portion of the heel is placed on the ground. This
is subsequently followed by the other parts of the heel and the sole of the foot is pressed on the
ground. The toes are the last to be pressed followed by the lifting of the foot making another step
forward. The pressure at the rear portion of the heel and in the region of the toes is the most
forceful, hence the impression is the most.
During the process of running the foot marks are less distinct because of the slipping of
the foot and the sand or soil thrown into the marks by the pressure of the tip of the toes.
Gait Patterns:
A scientific investigation of the gait pattern may be useful for purposes of identification
and investigation of the crime scene. Gait pattern is the series of foot marks by a person walking
or running. Examination of the gait includes the direction line, gait line, foot angle, principal
angle and the length and breadth of the steps.
e. Hands and feet — Size, shape and abnormalities of the hands and feet may be the bases
of identification. Some persons have supernumerary fingers or toes far apart with bony
prominence.
5. Weight — This is not a good point of identification for it is easily changed from time to time.
7. Birth marks — Birth marks may be a spot naevi, port wine, or a Mongolian blue spot. They
may be removed by carbon dioxide snow, electrocautery, or by excision. The marks must be
described as to shape, location, dimension, color and degree of pigmentation.
9. Moles — Ordinarily they are permanent but can be removed by electrolysis, by radium or by
carbon dioxide snow.
10. Scar – A remaining mark after healing of the wound. The fibrous tissue takes the place of the
original tissue which has been injured or destroyed. A scar is devoid of specialized tissues so it
does not contain pigment, sweat or sebaceous glands. Its number, exact location, size and shape,
and whether it is elevated or depressed should be noted.
Faint scars may be made visible by making the surrounding skin red upon applying friction with
hand or by heat.
Scar which develops after a secondary infection is usually marked.
Scar increases in size in proportion with the growth of the person.
Age of the Scar — A recently formed scar is slightly elevated, reddish or bluish in color, and
tender to touch.
In a few weeks to two months, the scar has inflammatory redness and it is soft and sensitive.
Two to six months later, it becomes brownish or coppery red in color, free from contraction and
corrugation, and soft.
When the scar is white, glistening, contracted and tough, it is not less than six months.
The period of scar formation may be delayed by sepsis, poor vascularity of the part involved,
age, depth of the wound, mobility, presence of foreign body and health condition of the victim.
Scar may or may not develop if the wound is small, superficial and healed by first intention.
Characteristics of the scar may show the cause of the previous lesion:
a. Surgical operation — Regular form and situation with stitch marks.
b. Bums and scald — Scars are large, irregular in shape, and may be keloid. Scar of scald may
show stippled surface.
c. Gunshot — Disc-like, depressed at center and may be adherent to the underlying tissue.
d. Tuberculosis sinus — Irregular in shape furrowed, with edges hardened and uneven.
e. Flogging — Fine white lines diagonally across back, depressed small spot at interval.
f. Gumma — Depressed scar following loss of tissue.
g. Lupus — Bluish-white scar.
h. Venesection — At bend of elbow, on dorsum of foot, or on temporal region.
i. Wet cupping — Short parallel scars on lower part of the back and loin.
11. Tribal marks — Marks on the skin by tattooing or branding. In branding heated metal is
pressed on the skin and during the healing process a scar develops as a mark. The tribal marks
are placed in the exposed parts of the body and used to identify person or membership of a tribe
or social group.
12. Sexual organ - Male organ may show previous circumcision. In a female the uterus and
breasts may show signs of previous pregnancy.
Previous gynecological operation may be seen in the abdomen.
13. Blood examination — Blood type, disease, parasitic infection or toxic substances present may
be utilized to distinguish one person from another.
A. FINGERPRINTING
Fingerprinting is considered to be the most valuable method of identification. It is universally
used because:
1. There are no two identical fingerprints:
Fingerprints show unlimited and infinite varieties of form.
Two or more fingerprints may grossly appear to be seemingly alike but under a microscope or
the magnifying lens, the difference may be proven. The chances of two fingerprints being the
same are calculated to be 1 to 64,000,000,000 which is ten times the number of fingers existing
in the world.
2. Fingerprints are not changeable:
Fingerprints are formed in the fetus in the fourth month of pregnancy. During the latter stage of
pregnancy as well as after birth, the pattern enlarges, but no changes take place in the number
and arrangement of the friction ridges.
The finger may be wounded or burned, but the whole pattern with all its details will reappear
when the wound heals. If the injury is deep or beyond the layers of the skin and scar develops, it
will not deter identification. On the contrary, the scar will make a much deeper impression of the
pattern. It can be said that fingerprints are an indelible signature which a person carries from the
cradle to the grave.
* Practical Uses of Fingerprints:
1. Help establish identity in cases of dead bodies and unknown or missing persons.
2. Prints recovered from the crime scene associate person or weapon.
3. Prints on file are useful for comparative purposes and for the knowledge of previous criminal
records.
Among illiterates, right thumbprint is recognized as a substitute for signature on legal
documents. Countries differ as to which finger is used for the purpose. India uses the left thumb,
Spain uses the right pointing finger.
* Dactylography is the art and study of recording fingerprints as a means of identification.
* Dactyloscopy is the art of identification by comparison of fingerprints. It is the study and
utilization of fingerprints.
* Poroscopy is the study of the pores found on the pappillary or friction ridges of the skin for
purposes of identification.
Kinds of Impressions:
1. Real impression — Impression of the finger bulbs with the use of printing ink on the surface of
the paper. Other coloring materials may be used but they are less visible and indelible.
2. Chance impression — Fingerprints which are impressed by mere chance without any intention
to produce it. Chance impression maybe:
a. Visible print — Impression made by chance and is visible without previous treatment.
Impression made by the fingers smearedwith some colored substances, like black ink, vegetable
juice, may be visible immediately after impression.
b. Plastic print — Impression made by chance by pressing thefinger tips on melted paraffin,
putty, resin, cellophane, plastic tape, butter, soap, etc.
c. Latent print — Prints which are not visible after impression but made visible by the addition of
some substances. Latent prints develop because the fingers are always covered with colorless
residue of oil and perspiration which when pressed on smooth and non-absorbent material will
cause the production of the prints.
The following substances are commonly used to make latent prints visible:
(a) Graphite for spraying
(b) Aluminum powder
(c) Plaster of Paris
(d) Copper powder for latent prints on leather
(e) Metallic antimony
(2) Chemical development by fuming and immersion:
Fuming by iodine or arsenic acid or immersion in a solution of silver nitrate may develop latent
prints.
How to Get Fingerprint Impressions on Dead Bodies:
In cases of fresh dead bodies, the fingers are unclenched and each one is inked individually with
the aid of a small rubber roller. The paper where the print will be impressed will be placed in a
spoon shaped piece of wood and slowly and evenly rolled over the pattern.
If the fist is too tightly clenched, a small incision may be made at the base of the fingers. The
contraction may also be overcome by dipping the hands in hot water.
If the so-called washerwoman's skin is not too marked on the fingerprints of dead bodies
recovered shortly from bodies of water (floaters), the fingers may be dried off with a towel and
glycerin is injected with a syringe under the skin of the finger tips in order to smoothen the
surface. The fingerprints are then taken like that of a fresh dead body.
If the "floater" has been in a body of water for a longer time and the friction ridges have
disappeared, the skin of the fingertips is cut away. This area of skin from each finger is placed in
a small labelled test tubes containing formaldehyde solution. If the papillary ridges are still
preserved on the outer surface, the person taking the prints places a portion of the skin on his
right index finger protected by a rubber glove and then takes the print after inking the finger tip.
The same procedure as described may be applied to putrefied or burned bodies according to
circumstances.
Types of Fingerprint Patterns:
1. Arches — The ridges go from one side of the pattern to another, never turning back to make a
loop.
a. Plain arches — The ridges on one side of the impression and
flow or tend to flow out the other side with rise or wave in the center.
b. Tented arch — One or more ridges at the center to form a definite angle of 90 degrees or less
than 45 degrees from the horizontal plane.
2. Loops — One or more ridges enter on either side, recurves and terminate or tend to terminate
on the same side from which it entered.
a. Ulnar loop — Recurves towards the ulnar side of the hand or little finger.
b. Radial loop — Recurves towards the radial side of the hand or thumb.
3. Whorls — Patterns with two deltas and patterns too irregular in form to classify:
a. Simple whorls — Consist of two deltas with a core consisting of circles, ellipses, or spiral
turning to the right or left.
b. Central pocket loop- — It is like simple loop but in the core, one may find one ridge which
forms a convex towards the opening of the loop.
c. Lateral pocket loops — There are at least two loops opening at the same side.
d. Twin loop — There are at least two loops opening at the different sides.
e. Accidentals — There are no rules that can be made in this pattern. They are rare and often with
more than two deltas.
As long as the dermis of the bulbs of the finger is not completely destroyed, the
fingerprints will always remain unchanged and indestructible.
The role of the teeth iri human identification is important for the following reasons:
1. The possibility of two persons to have the same dentition is quite remote. An adult has 32
teeth and each tooth has five surfaces.
Some of the teeth may be missing, carious, with filling materials, and with abnormality in shape
and other peculiarities. This will lead to several combinations with almost infinite in number of
dental characteristics.
2. The enamel of the teeth is the hardest substance of the human body. It may outlast all other
tissues during putrefaction or physical destruction.
3. After death, the greater the degree of tissue destruction, the greater is the importance of dental
characteristics as a means of identification.
4. The more recent the ante-mortem records of the person to be identified, the more reliable is
the comparative or exclusionary mode of identification that can be done.
In order to make an accurate dental record available for purposes of comparison with that of the
person to be identified, Presidential Decree No. 1575 was promulgated, requiring practitioners of
dentistry to keep records of their patients. It provides the following:
"Whereas, the identification of persons is a necessary factor in solving crimes and in
settling disputes such as claims for damages, insurance, and inheritance; _
Whereas, in these cases where the identification of persons cannot be established through
the regular means, identification through dentition has been proven to be necessary and effective;
Whereas, however, records of dentition of persons are often not available due to the lack
of systematic recording of dental practitioners of the dental history of their patients.
For purpose of uniformity, the following are the description of location for dental identification:
1. Teeth position:
a. Anterior — From cuspid to cuspid inclusive (it includes cuspid, lateral and central incissor).
b. Posterior — All bicuspid and molar teeth.
2. Surface:
Occlosal — O — Surface which is in contact with the opposing teeth when jaws are in occlusion
(closed).
Mesial — M — Surface in direct contact with the adjacent tooth towards the midline.
Distal — D — Surface in direct contact with the adjacent teeth away from the midline.
Buccal — B — Surface facing the lip or cheek.
Lingual — L — Inward directed surface of the teeth.
3. Restoration:
Amalgam (silver filling), gold inlay, gold foil, silicate, acrylic, temporary cement, crown.
4. Prosthesis:
a. Fixed prosthesis — bridge
b. Removable prosthesis:
(1) Complete denture
(2) Partial denture
5. Root canal treatment (endodentia).
2. Age
9 yrs 12 permanent teeth (8 incisors and 4 molars).
11 yrs 20 permanent teeth (8 incisors, 8 premolar and 4 molar).
13 yrs 28 permanent teeth and no deciduous teeth.
8 to 10 yrs Calcification begin at the 3rd molar.
25 yrs Root-ends of 3rd molar completely calcified.
Beyond 25 yrs. . . . Ends of the root of the 3rd molar have been completely calcified.
After 30 yrs Carries frequently develop at the cementum.
There may be gingival recession, decay attack
of the root surface.
3. Sex
Examination for the presence of Barr bodies from palatal
scrappings. j
C. HANDWRITING
A person may be identified through his handwriting, handprinting and handnumbering.
Sec. 23, Rule 132, Rules of Court — Handwriting, how proved:
The handwriting of a person may be proved by any witness who believes it to be the handwriting
of such person, and has seen the person write, or has seen writing purporting to be his upon
which the witness has acted or been charged, and has thus acquired knowledge of the
handwriting of such person. Evidence respecting the handwriting may also be given by a
comparison, made by the witness or the court, with writings admitted or treated as genuine by the
party against whom the evidence is offered, or proved to be genuine to the satisfaction of the
judge.
The genuiness of any disputed writing may be proven by any of the following ways:
1. Acknowledgement of the alleged writer that he wrote it; Statement of witness who saw
the writing made and is able to identify it as such;
By the opinion of persons who are familiar with the handwriting of the alleged writer, or by the
opinion of an expert who compares the questioned writing with that of other writings which are
admitted or treated to be genuine by the party against whom the evidence is offered.
Sec. 44, Rule 130, Rules of Court — Opinion of ordinary witnesses:
The opinion of a witness regarding the identity of handwriting of a person, when he has
knowledge of the person or handwriting; the opinion of a subscribing witness to a writing; the
validity of which is in dispute, repecting the mental sanity of a person, 6he reason for the opinion
being given, may be received as evidence.
* Movements in Writing:
1. Finger movement — The letters are made entirely by the action of the thumb, the pointing and
middle fingers. Such is found among in dispute, respecting the mental sanity of the signer; and
the opinion of an intimate acquaintance respecting the mental sanity of a person, the reason for
the opinion being given, may be received as evidence.
In order for an ordinary witness to be qualified to express his opinion, it must be shown
that he has some familiarity with the handwriting of the person in a way recognized by law.
2. Hand movement — The letters are produced by the action of the hand as a whole with the wrist
as the center of action and with some action of the fingers. Most of the illegible, scratchy and
angular writings of women are produced by such movement.
3. Arm movement — The movement in writing is made by the hand and arm supported with the
elbow at the center of the lateral swing. Many of the good writings are written in this manner.
There is more speed, rhythm and freedom in this way of writing.
4. Whole arm movement — The action is produced by the entire arm without any rest. The source
of motion comes from the shoulder. Writing on a blackboard is a good example of whole arm
movement.
The Form, Style and Characteristics of the Handwriting of a Person are Basically Determined
By:
A.Primary factors:
1. Survival of the letters are formed when a person begins to write.
Children who were under the same tutelage during their initial period of learning how to write
have the tendency to develop similar writing habits.
2. Inclusion of some characteristics due to admiration of a peculiar design in writing.
3. Identifying characteristics may be the result of the great volume of writing done.
4. The presence or absence of physical abnormalities or defects originating from illness, injury,
psychological variations and other similar conditions.
B. Secondary factors:
1. The position of the writer, e.g. sitting, standing, lying, arm high or low, and other similar
variations not normally observed in his ordinary writing habit.
2. Temporary physical or psychological disturbances, such as excitement, fear, pain, exhaustion,
injury to thd hand or arm, etc.
3. Other external temporary variables, such as writing without glasses, bad lighting, irregular
surface, external interference.
4. Physical and chemical factors:
a. Writing instrument:
(1) Ballpen — It. usually leaves rounded line showing no tip separation even when pressed
heavily. Smudge may be deposited on the line. The ink, not being a true liquid, does not flow
into the fibers and spread in the same way as fluid ink does.
(2) Fountain pen — The lines are more or less round but when pressure is increased there is
separation of the nib which is easily detected. There is evenness in the flow of ink.
(3) Steel pen — There is unevenness in the flow of ink and leaves a scratchy appearance.
(4) Pencil — Lead of pencils is compose of graphite and clay with kaolin as binder. Soft pencils
have greater proportion of graphite while hard ones have relatively more clay. Cheap quality
pencils have frequently gritty impurities which scratch the paper, while high-grade pencils are
free from such grit.
Paper:
(1) Color — Color can be well appreciated with a good light.
Dirt, stain or fading condition may not show the true
color of the paper.
(2) Surface appearance — It may be smooth or rough. The surface may be damaged or wrinkled.
(3) Watermarks - Exposure of the paper to a strong light may reveal the watermarks of the
manufacturer or the type of paper.
(4) Weight and thickness — The thickness may be measured by means of the paper micrometer.
Papers are designated in weight which is in turn related to the thickness of the sheet.
Ink:
(1) Iron gallotannate ink — Commonly used in "blue-black" ink and still the basis of the greatest
number of commercial ink. The changes in the paper may provide some indications of the age of
the writing.
(2) It may be a solution of a single or a mixture of dyes.
This is a common constituent of "washable" inks.
(3) Logwood ink — Made of logwood extract with salts of iron, copper, or chromium.
(4) Carbon ink — It is a fine suspension in water of carbon with stabilizing agent. India ink is an
example of this type of ink.
(5) Ballpoint ink — A thick suspension of dye in a liquid which is usually a drying oil
Disguised Writing:
Disguised writing is the deliberate attempt on the part of the writer to alter his writing
habit by endeavoring to invent a new writing style or by imitating the writing of another person.
The principle of identification of handwriting is also applicable to hand printing and hand
numbering.
Typewriter Identification:
The identification of the typing machine used in a questioned document, like that in ballistics
examination, may be on the basis of:
1. Class characteristics — those characteristics which serve to distinguish it from any other
machine, such as:
a. Manufacturer's characteristics
b. Size and design of the type
c. Line and letter spacing
2. Individual characteristics:
a. Defects in the type face — Unusual manner of letter formation due to factory defect, misuse of
the machine or wear and tear.
b. Defects in the alignment — Malpositioning spacing and alignment may be modified by
loosening of the hinges and positioning of the letters on account of wear and tear and changes in
the spring pressure.
c. Other machine defects:
(1) Skipping space
(2) Irregular margin stops
(3) Improper letter spacing
(4) Improper ribbon actions
A typewriter has 44 keys with 88 characters, each operating independently of one another and
each being capable of damage or having inherent defects. Consequently, a variety of
combinations of these defects may be the basis of typewriter identification.
The questioned document may be compared with those made by the suspected typewriters.
How to Determine Whether the Remains Comes from a Single Individual or Not:
A complete lay-out of the bones on a table in their exact locations in the human body is
necessary. Any plurality or excess of the bones after a complete lay-out denotes that the remains
belong to more than one person. However, congenital deformities as supernumery fingers and
toes must not be forgotten. The unequality in sizes, especially of the limbs may be ante-mortem.
Height:
Several formulae using different constants have been forwarded in the approximation of
the height of a person by measuring the long bones of the body.
1. The femur is measured from the head to the apex of the inner condyle. If the femur has been
measured in the oblique position and not straight, add 0.23 for male and 0.33 for female to the
length before using the above formulae.
2. The tibia is measured from the upper articular surface to the tip of the malleolus. If the tiDia
has been measured with, and not without, the spine, subtract 0.96 for male, and 0.87 cm. for
female, from the length before using the above formulae.
3. The humerus and radius are measured in their greatest length.
(Taylor's Principles and Practices of Medical Jurisprudence, S. Smith, 10th ed., Vol 1, p. 155).
4. Inasmuch as the formulae for male and female skeletons are different, it is necessary to
determine the sex of the skeleton before the formulae may be applied.
C. Stature from bone:
Dupertuis and Hadden's General Formulae For Reconstruction of
Stature From Lengths of Dry Long Bones Without Cartilage (Constant Terms in Metric and
Adapted to English System)
D.Topinard and Rollet, two French anatomists devised a formula for the determination of the
height for males and females.
Female
1. Lighter construction wall less pronounced.
2. Height lesser and flays off its wall less pronounced.
3. Pubic arch wider and rounder.
4. Diameter of the true pelvis greater.
5. Curve of the iliac crest is of the lower level.
6. Wide greater sciatic notch.
7. Body of the pubis wider.
8. Iliopectineal line rounded.
9. Obturator foramen triangular.
10. Sacrum long and wide.
B. Sternum:
C. Femur:
D. Humerus:
E. Cranium:
The following points may be used in determining the race in the remains of a person:
A. Extrinsic Factors:
1. Color of the skin
2. Facial features
3. Nature of the hair
4. Mode of dressing
B. Indices:
1. Skull:
2. Pelvis
Superimposed Photography:
This is a special method of determining the person to whom the skull belongs. The
negatives of the picture of the skull and the suspected individuals are superimposed and printed.
This will show whether the contour of the skull fits the contour of the face of the suspected
person.
E. DETERMINATION OF SEX
Evidences of Sex:
1. Presumptive evidences:
a. General features and contour of the face.
b. Presence or absence of hair in some parts of the body.
c. Length of the scalp hair. Generally, the female has long hair in the scalp than that of the male.
d. Clothes and other wearing apparel, but not in a transvestite.
F. DETERMINATION OF AGE ^
Legal Importance of Determination of Age:
As an aid to identification:
Mention of the age of the wanted or missing person will create an impression of the
physical characteristics, social life and psychic and mental behavior of that person. Although it
may only be presumptive, it may be useful in identification.
Determination of criminal liability:
Art. 12, Revised Penal Code — Circumstances which exempt from criminal liability — The
following are exempted from criminal liability:
1.
2. A person under nine years of age.
3. A person over nine years of age and under fifteen, unless he has acted with discernment, in
which case, such minor, shall be proceeded against in accordance with the provisions of article
80 of this code.
The person who has reached majority is qualified for all acts of civil life, save the
exceptions established by this Code in special cases. (Art. 402, Civil Code).
Infanticide — The penalty provided for parricide in article 246 and for murder in article 248 shall
be imposed upon any person who shall kill any child less than three days of age (Art. 255,
Revised Penal Code).
Seductions:
Qualified seduction — The seduction of a virgin over twelve years and under eighteen years of
age, committed by any person in public authority, priests, house-servant, domestic, guardian,
teacher, or any person who, in any capacity, shall be entrusted with the education or custody of
the woman seduced, shall be punished by prision correccional in its minimum and medium
periods (Art. 337, Revised Penal Code).
Simple seduction — The seduction of a woman who is single or a widow of good reputation, over
twelve but under eighteen years of age, committed by means of deceit, shall be punished by
arresto mayor (Art. 338, Revised Penal Code.
d. Consented abduction — The abduction of a woman victim over twelve and under eighteen
years of age, carried out with her consent and with lewd designs shall be punished by the penalty
of prision correccional in its minimum and medium period (Art. 343, Revised Penal Code).
PHYSICAL EXAMINATIONS:
1. Solubility test:
Recent blood shed is soluble in saline solution and imparts a bright red color.
Stains which have been exposed to air become dry; haemoglobin is transformed to meth
hemoglobin or hematin. If the stain hasbeen kept in damp places for a long time; hemoglobin is
transformed to hematin.
2. Heat test:
Solution of the blood stain when heated will impart a muddy precipitate.
3. Luminescence test:
Stains on dark fabric mixed with mud, paint, etc. emit bluishwhite luminescence in a dark room
when sprayed with one of the two solutions:
a. 3-amino-phthalic-acid-hydrazide-HCL 1 gram
Sodium peroxide 5 grams Distilled water 1,000 cc.
b. 3-amino-phthalic-acid-hydrazide-HCL 1 gram
Sodium carbonate 50 grams
Hydrogen peroxide (10 Vol.) 50 grams Distilled water 1,000 cc.
The substance responsible for the reaction is hematin. Older stains therefore react better than
new ones. Although the solutions are said not to interfere with further tests, unsprayed specimen
of the material must be kept for the serologic test. (Lyon's Medical Jurisprudence for India by
S.P.S. Greval, 1953, p. 303).
CHEMICAL EXAMINATIONS:
1. Saline extract of the blood stain plus ammonia will give a brownish tinge due to the formation
of alkaline hematin.
2. Benzidine test:
A piece of white filter paper is pressed firmly on the suspected stain. Benzidine reagent is
dropped on the paper, then followed by drops of active hydrogen peroxide. A positive result will
show blue color. A positive result is not conclusive, because an oxidizing agent will give a
positive blue color reaction. Benzidine test has the sensitivity up to 1:300,000 dilution.
Benzidine reagent:
Benzidine sulphate is dissolved in glacial acetic acid to form 10% solution.
3. Guaiacum test (Van Deen's Dyas' or Schombein's Test):
To a white filter paper pressed and rubbed on the surface of the stain, the solution of the
alcoholic tincture of guaiacum is added and then hydrogen peroxide or ozonic ether is applied by
drops.
If blood is present, a blue color is imparted by the mixture. It is not conclusive like the benzidine
test because potato skin, iron rust, cheese, blue and indigo may give a positive reaction to the
test. The guaiacum test is positive up to 1:5,000 dilution.
4. Phenolphthalein test (Kastle-Meyer Test):
A drop of the Kastle-Meyer's reagent is dropped on a white filter paper with the stain and left for
at least ten seconds. A positive result will show a pink color after the addition of hydrogen
peroxide. This test is not conclusive but sensitive up to
1:80,000,000 dilution. This test proves only the presence of peroxidase.
Kastle-Meyer's reagent:
5. Leucomalachite Green test:
This test which was recommended by Adler in 1904 is quite useful, but it is not so sensitive as
the benzidine test. It depends upon the fact that leucomalachite green is oxidized to malachite
green with a bluish-green or peacock-blue color by hydrogen peroxide solution. The reaction
occurs also with a solution of the blood pigment previously boiled. On the other hand, the
reaction is negative when iron is removed from haemoglobin forming hematoporphyrin.
MICROSCOPIC EXAMINATIONS:
Saline extract of the stain is examined under the microscope.
Note the presence of red blood cells, leucocytes, epithelial cells and microorganisms. The
presence of red blood cells will conclusively show that the stain is blood. By microscopic
examination, we can differentiate the origin or the part of the body it came from. Menstrual
blood will show abundance of vaginal epithelial cells and Doederlein's bacilli.
MICRO-CHEMICAL TESTS:
1. Hemochromogen crystal or Tokayama test:
A fragment of the suspected material is placed on a slide glass and a drop of hemochromogen
reagent is added. A cover glass is placed on top and heated gradually for a time, then examined
under the microscope. Crystals varying from salmon color to dark brown and pink and which are
irregular rhomboids or in clusters, may be seen. This test is positive to any substance containing
hemoglobin.
2. Teichmann's blood crystals or Hemin crystals test:
On the microscopic slide is placed fragments of the stain and a drop of water with trace of
sodium chloride added. Add glacial acetic acid and evaporate to dryness under a cover slip. Dark
Drown rhombic prisms of chloride of hematin are formed. This is considered as the best of the
micro-chemical test.
3. Acetone-haemin of Wagenhaar test:
A particle of dried stain or a fiber of the stained fabric is placed on a glass slide and covered with
a cover slip with a needle interposed to prevent direct contact of the cover slip with the slide.
A drop of acetone is run under the cover slip so that the material is surrounded and a drop of
diluted oxalic or acetic acid is then added. When examined under high power microscope, small
dark, dichroic acicular crystals of acetone-haemin are seen.
SPECTROSCOPIC EXAMINATIONS:
This examination depends on the principle that blood pigments have the power to absorb
light of certain wave length and produce certain characteristic absorption bands on the spectrum.
By means of the spectroscope we can determine the presence of the following substances:
1. From fresh blood stains:
a. Oxyhemoglobin
b. Hemoglobin
c. Reduced hematin or hemochrogen
2. From older stains:
a. Methemoglobin
b. Alkaline hematin
c. Hematoporphyrin
d. Reduced hematin
3. Other blood preparations:
a. Acid hematin
b. Alkaline hematin
c. Carboxyhemogiobin
d. Hematin
BIOLOGIC EXAMINATIONS:
1. Precipitin test:
This test is to determine whether the blood is of human origin or not.
Principle of the test:
By injecting an animal, usually, a rabbit, with defibrinated blood of unrelated animal, an anti-
serum is produced in the blood of the animal injected. The serum of this animal injected is
capable specifically of precipitating the serum of the unrelated animal whose blood serum has
been injected. However, closely related animals may also give the same response.
Preparation of the anti-human serum:
A dose of 1.5 cc. to 2.0 cc. per kilogram body weight of human defibrinated blood is
injected intravenously in the marginal vein of the rabbit's ear. The dose is then repeated every
third day with three or five injections. The titre of the rabbit serum is tested with the human
serim. If the anti-human rabbit serum has sufficient power to produce a ring of haziness at the
junction of the two sera, then the titre is sufficient for the examination of the unknown.
Some biologists prefer combined intravenous and intraperitoneal injection of the serum
but the result is the same.
If the titre is sufficiently strong the rabbit is bled to death and the serum is oollected for the
examination of the unknown.
2. Blood grouping:
Grouping is true not only with blood but also with other fluids of the body like saliva,
vaginal secretion, seminal fluid, milk, urine and others.
Age of the Blood Stains:
When blood is exposed to the atmosphere or some other influences, its hemoglobin is
converted to meth-hemoglobin or hematin.
The color is changed from red to reddish-brown. The presence of acid accelerates the
formation of hematin. These changes take place in warm weather within 24 hours. Blood of one
week old and that of six weeks may not present a difference in physical and chemical properties.
The fibers have typical large cavities which are not uniform but vary with the degree of
contraction of the walls of the fibers which are lignified.
The ends are blunt.
The fibers are stained red with phloroglucin and yellow with aniline sulphate, also with
iodine and sulfuric acid.
6. Wool Fibers:
These fibers can easily be distinguished from vegetable fibers since the former show an
outer layer of flattened cells and imbricated margins.
The interiors are composed of fibrous tissues but sometimes the medulla is present.
They do not dissolve in a solution composed of copper sulphate, sodium carbonate and
ammonia.
Stain is yellow with iodine and sulfuric acid and also with picric acid.
Do not dissolve in sulfuric acid.
Smell of singeing on burning.
7. Silk Fibers:
Manufactured silk is almost structureless, microscopically.
Fibers stain is brown with iodine and sulfuric acid and yellow with picric acid.
They dissolve slowly in a mixture of copper sulphate, sodium carbonate and ammonia.
8. Linen Fibers:
Fibers are straight and tapering to a point.
Cortical area shows transverse lines which frequently intersects, simulating a jointed
appearance.
The medullary region shows a thin dense line.
They do not dissolve in concentrated sulfuric acid.
If placed in 1% alcoholic solution of fuchsin and then in a solution of ammonium
hydroxide, they assume a bright red color (Medical Jurisprudence and Toxicology by Glaister,
8th ed„ P. 110).