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US005766930A. United States Patent 19 1] Patent Number: 5,766,930 Kobr 445] Date of Patent: Jun. 16, 1998 [54] METHOD OF BIOTREATMENT FOR SOLID SUz198 71982 Baetey et 18083 MATERIALS IN A NONSTIRRED SURFACE asset aioe ay ~ eal he 5236677 8/1993. Torres-Candonia etal woven 423/230 5248493 971993 Bredey eal sa (75) Inventor: William J. Kobr, San Mateo. Calif. 5245,486 9/1903 Briedtey et al. 43 , 7 S246a85 5/1993 Bnetey eta oncccones 42927 [73] Assignee: Geobiotics, Inc, Hayward. Calif. pea ee eee aa Sasini7 71995 . as (21) Appl. No. 636,117 S627 10395 “eset " S578593 117996 sma 22] Filed: Apr. 22, 1996 5.611.839 3/1997. ‘TS2 Related US. Application Data FOREIGN PATENT DOCUMENTS stom pan of Set. No vn 0522978 Al 10/1992 European Pat Off (63) Cf ia conanicnin ano St No 4621 Jan 2180829 41987 Uni Kingdom... C22B 108 1988, OTHER PUBLICATIONS [51] Int CLé ... ~ CRAB M18; C22B 1U00 ssc. 1 otal, Reda Poteniat-Controled Bacterial Sterne nen. L. et al. Redox Potential-Controlled Bacteria , MS HD REA Leaching Of Cholcogyrite Ores, Bichydrometalurgical (52) US. Cl. SS ela atTa ‘Technologies. The Minerals, Metals & Materials Society 75(74s, 423/22; 423/24; 425/27: aaron, Teebwlosies. The | 2 y sEabiG. ets: anne, SEDI SISTA tee, AOS: Bercy. C.L.. Mineral Blo-Processing: Oppornnites in 158) Ftd of Sete vrnrnn 9/8, 262. setve Metallurgy and Envronental Con NIST. 357264, 2625, 281, 280, 42372, 24.27, NOW NSO. ID 29, DIG. 17; 757712. 743. 744; C13P 3/00; ales te He Lecce C22B 3/18; BOLD 53/00; C10G 32/00; rao res COB 3/00 List continued on next page (56) References Cited 0 a Primary Examiner—Werber J. Liling US. PATENT DOCUMENTS ‘Attomey, Agent, or Firm—Lyon & Lyon LLP M1074 77992 Lazarff ta 588,476 8/1897 Rhodes (57) ABSTRACT 327001 12197 Lankenau etl. vote . 3,796,308 3/1974 Mellhinney et a. area mice EEG Sara Mlaieatecuere al undesired compound using a nonst face bioreactor is e051 4ns76 Pomel provided. According to the method the surface of a plurality 4017300 4/1077 Jonson fof coarse substrates is coated with a solid material to be Sos6261 177 biotreated to form a plurality of coated coarse substates. sansig 11979 The coarse substrates have a particle size greater than about {B65 “MS Honea $2327 0.3 em andthe solid material tobe biokeatd has a pstile nen et size less than at nonstirred surface reactor Taos Soa Netecy for SE Johan tee 250 um, nna ce ecr f 4270808 #2309 lpeuates ito heap of plang the lrally of coated A3a890 “jo3m79 coarse substrates into a tank so that the void volume of the Pent che ‘ars _eactor is greater than or equ to about 25%, The reactor i 4307m fiom 3 “23/1 inoculated with a microorganism capable of degrading the 4374007 27983 “23722 undesired compound in the solid material, and the solid Saozaa1 97983 2101606 material is ten bioteated inthe surface bioreactor until the 4424194 1/1984 4231 undesired compound in the solid material is degraded to a 4326615 T9e5 78101 desired concentration. Preferably the thickness ofthe solid AS57905 w2/96s ($2327 material coating on the plurality of coarse substrates is less A596) ANG Bruyneeyn ct —ovcnws 489262 Thin abou] tn and She WoL volume of th Teacon Is Manas Rane iomaeeca “auay greater than or equal to about 35%. The process fs useful for 4721526 1/1988 Elmore etal. 75/118 many different biotreatment processes. including the biore- 320788 nd88 Hutchins el Z5ni8 mediation of contaminated sels the desulfurization of coal. 4740249 41988 Kreb-Yull ea 75/101 and the biooxidation of refractory sulfide ores and concen- 4752392 1988 Waetal won 75/101 tates. In biremediaton applications. the undesired com- 4778319 soi988 Pec - F816 pount is yplcally an cepane compound: Io coat dosutan L789481 12/988 Buiedey 210/661 [zation and refractory sulfide ore biooxidation applications. Age cama oto Becks chat ~ 438/262 the undesired compound is sulfide minerals. 4987081 "1901 Hackl etal © fsa8 3006320 4/1991 Reid et a * fa3niso 5007 620. 41991 Emmet. Ie cal oes 90 Claims, 9 Drawing Sheets 5,766,930 Page 2 (OTHER PUBLICATIONS Fraser, K.S., et al.. Processing of Refractory Gold Ores, ‘Minerals Engineering, vol. 4, Nos. 7-11. pp. 1029-1041, 1991, Harrington. G..ct al. Engineering Aspects Of Heap Biooxi- dation Of Course-Crushed Refractory Gold Ores, Biohy-

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