Certificate of Analysis

CAFFEINE USP
CERTIFIED REFERENCE MATERIAL
(SECONDARY REFERENCE STANDARD)

1H-Purine-2,6-dione, 3,7-dihydro-1,3,7-trimethyl-

CERTIFIED PURITY: 99.5%

(Assay on as is basis)
NOMINAL PACKAGE SIZE: 2g
CATALOG #: 1000050 Lot #: PADM050
ISSUE DATE: 17 June 2016 CAS #: 58-08-2

Note: Certificates may be updated due to Pharmacopeial Lot change or the availability of new data.
Check our website www.padmlab.com for the most current version.
CRM EXPIRATION: 16 June 2017
RECEIPT DATE:_______________
Note: This space is provided for convenience only and its use is not required.
STORAGE: Store at room temperature in a well closed container.
CHEMICAL FORMULA: C8H10N4O2 Molecular weight: 194.19
PHYSICAL DESCRIPTION: White crystalline powder
THERAPEUTIC CATEGORY: CNS Stimulant
HAZARDS: Read Safety data sheet before using. All chemical reference materials should be considered
potentially hazardous and should be used only by qualified laboratory personnel.
INSTRUCTIONS FOR USE: Do not dry, use as is. The internal pressure of container may be slightly
different from the atmospheric pressure at the user’s location. Open slowly and carefully to avoid
dispersion of the material. This material is intended for Analytical use only. Not for drug, human
consumption, household or other uses.

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TRACEABILITY ASSAY
Comparative assay demonstrates direct traceability to Pharmacopeial Standards specification: 98.5-101.0%
(anhydrous), United States Pharmacopeia (USP).
REFERENCE STANDARD DETAILS: Caffeine USP, Lot No., KOK210 Labeled Content- 99.9% on as
is basis
METHOD:
Column:15cm x 4.6mm, 5um, Packing L1
Mobile Phase: Acetonitrile: Tetrahydrofuran: Buffer (0.82 g/L of anhydrous sodium acetate) (25:20:955)
Adjust pH to 4.5 with glacial acetic acid.
Flow Rate : 1.0mL/min
Injection : 10uL
Detector : 275nm
ASSAY vs. USP REFERENCE STANDARD (as is basis)
ASSAY VALUE

99.5%
Representative Chromatogram from Lot: K0K210 Analysis

Representative Chromatogram from Lot: PADM050 Analysis

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Water Determination (Method III[921])
Method: Dry in an oven at 80°C for 4h
Loss = 0.21%

IDENTIFICATION TESTS
INFRARED SPECTROPHOTOMETERY (Comparative identification analysis demonstrates direct
traceability to Pharmacopeial standards)
Representative IR spectrum from Lot: K0K210 Analysis

Representative IR spectrum from Lot: PADM050 Analysis

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MASS SPECTROSCOPY
Representative Mass spectrum from Lot: PADM050 Analysis

m/z 195.2[M+1]+
REMARKS
All the above parameters meets the requirements of USP monograph.

HEAD-QUALITY

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