BQ-PR Fosfatasa Alcalina

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CÁLCULO DE LA CONCENTRACIÓN DE SUSTRATO (mM)

TUBOS SUSTRATO (M) SUSTRATO (mM) C1 (M)


1 0.00E+00 0.00E+00 V2
2 2.14E-05 2.14E-02
3 4.29E-05 4.29E-02
4 6.43E-05 6.43E-02
5 1.07E-04 1.07E-01
6 5.36E-04 5.36E-01
7 1.07E-03 1.07E+00

CÁLCULO DE LA ACTIVIDAD ENZIMÁTICA

TUBOS ABSORBANCIA ACT. ENZ. (mM/L)


1 0 0.00000
2 0.26 0.17680
3 0.355 0.24140
4 0.481 0.32708
5 0.511 0.34748
6 0.754 0.51272
7 0.602 0.40936

MODELO MICHAELIS MENTEN MOD


0.60000
TUBOS SUSTRATO (mM) ACT. ENZ. (mM/L)
0.50000
1 0.0000 0.00000 Activ. Enz. (mM/L)
0.40000
2 0.0214 0.17680
3 0.0429 0.24140 0.30000
4 0.0643 0.32708
5 0.1071 0.34748 0.20000
6 0.5357 0.51272 0.10000
7 1.0714 0.40936
0.00000
0.0000 0.2000

MODELO LINEWEAVER BURK

TUBOS 1/SUSTRATO (mM) 1/ACT. ENZ. (mM/L)


MOD
6
1 0 0 f(x) = 0.094581198403
2 46.6666666666667 5.65610859728507 R² = 0.8103758254103
5
3 23.3333333333333 4.14250207125104
1/Activ. Enz. (mM/L)

4 15.5555555555556 3.05735599853247 4
5 9.33333333333333 2.87786347415679
3

1
R² = 0.8103758254103
5

1/Activ. Enz. (mM/L)


4

3
6 1.86666666666667 1.95038227492589
7 0.93333333333333 2.44283760015634 2

0
0 5 10
0.012
11.2

MODELO MICHAELIS MENTEN


0.60000

0.50000
Vmax
0.40000
0.52
0.30000

0.20000

0.10000

0.00000
0.0000 0.2000 0.4000 0.6000 0.8000 1.0000 1.2000
Sustrato (mM)

MODELO LINEWEAVER BURK


6
f(x) = 0.094581198403764 x + 1.55535969063776 m
R² = 0.810375825410365 b
5

4 Vmax
0.642921435
3

1
R² = 0.810375825410365
5

0
0 5 10 15 20 25 30 35 40 45 50
1/Sustrato (mM)
Km
4.00E-02

0.0946
1.5554

Km
0.06082037

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