Notas random de biofisicoquimica

We might expect the rate of a reaction to depend on two quantities: the number of collisions
per second and the fraction of collisions that activate molecules for the reaction.
Both quantities should increase with increasing temperatura

Arrhenius (1859- 1927) discovered that the temperature dependence of many reactions
could be described by the following equation:

where A and Bare reactants and xi represents an activated complex. A fundamental

assumption of transition-state theory (and one that differentiates it from collision theory) is
that the reactants are always in equilibrium with xi. The activated complex should not be
thought of as a stable, isolatable intermediate because it is assumed to be always in the
process of decomposing

The principle of microscopic reversibility states that at equilibrium, the rates of the forward
and reverse processes are equal for every elementary reaction occurring.* It means that the
process A → B is exactly balanced by B→A

As we shall see later, because the frequency factor A is related to molecular collisions, it is
temperature dependent. For a limited temperature range (< 50 K), however, the
predominant temperature variation is embraced by the exponential term

Es habitual medir la velocidad inicial (v0) de una reacción para minimizar las reacciones
reversibles y la inhibición de enzimas por los productos.
La velocidad inicial corresponde a una concentración de sustrato fija conocida. A medida
que pase el tiempo, la concentración de sustrato disminuirá

La velocidad aumenta de manera rápida y lineal con (S] a bajas concentraciones de

sustrato, pero se reduce gradualmente hacia un valor límite a altas concentraciones de
sustrato. En esta región, todas las moléculas de enzima están unidas a las moléculas de
sustrato, y la velocidad se convierte en Orden cero en la concentración del sustrato.

La relación entre Vo y [S] se puede representar mediante la ecuación de una hipérbola

rectangular

to explain the dependence of the initial rate of enzyme-catalyzed reactions on concentration

se considero el siguiente sistema: