Risk

BSL Level Goal of BSL Practices Facilities, Equipment

Group

Basic None required; open

Good Microbiological
1 benchwork, directional
BSL 1 Techniques (GMT)
airflow

Open bench plus

Basic Level 1 plus Biological Safety

Personal
2 protective clothing, Cabinet (BSC) for
BSL 2 protection
biohazard signage aerosols, autoclave
access

Level 2 plus special BSC and/or other

clothing, controlled primary containment
3 BSL 3 Containment
access, medical devices for all activities,
screening negative pressure

Level 3 plus airlock Class III BSC or positive

Maximum
entry, shower exit, pressure suits, double
4 BSL 4 Containment,
special waste door autoclave, filtered
Isolation
disposal air and exhaust