American Journal of Medical Genetics 108:192~197 (2002) Delineation of the dup 5q Phenotype by Molecular Cytogenetic Analysis in a Patient With dup5q/del 5p (Cri du Chat) Brynn Levy,'“* Teresa M. Dunn,' Jeffrey H. Kern,* Kurt Hirschhorn,'” and Nataline B. Kardon!” "Department of Human Genetics, Mount Sinai School of Medicine, New York, New York “Department of Pediatrics, Moun? Sinai School of Medicine, Now York, New York ‘Department of Pediatrics, Flushing Hospital Medical Center, Queens, New York An infant girl presented with multiple congenital abnormalities and a distinctive mewing cry. Her karyotype was 46,XX,add5p. Chromosome analysis on the mother re- vealed an apparently balanced pericentric inversion of chromosome 5, with the precise position of the breakpoints not clearly dis- cernable by GYG banding, 46,XX.inv(5) (p15.2/32485.12). Fluorescence in situ hybri- dization (FISH) studies using a commercial i du chat probe (D5S721,D5823) revealed signals on both the normal and derivative chromosomes. Telomerie probes specific for 5p and 5q were used to confirm the peri- centric inversion in the mother and demon- strated the loss of the terminal 5p region and a duplication of the terminal 5q region in the proband. The imbalance on chromo- some 5 in the patient was further defined using comparative genomic hybridization (CGH), which revealed a loss of material from 5p15.3—pter and a gain of 5q34— qter. ‘The presence of the eat-like ery appears to be the only specific feature that can be linked to the loss of 5p material. The remaining dysmorphie features of this infant appear to be due specifically to the duplication of the 5q sequences. The combi- nation of FISH, CGH, and cytogencties has confirmed that the characteristic ery of the eri du chat syndrome is due to the deletion of the most distal part of the classic del 5p Grant sponsor: March of Dimes; Grant number: FY0A06; Grant sponsor: National Institute of Child Health and Human Development; Grant number: 5 T32 HDO7105-24 "Correspondence to: Dr. Brynn Levy, Department of Human CGenoties, Box 1497, Mount Sinal Sebool of Meteine, One Gustave Levy Blace, New York, NY 10028. Exmail Brynn levyyimssin eda Received 25 January 2001; Accepted 25 November 2001 Published online 6 Februasy 2002 (© 2002 Wiley-Liss, Inc. ‘DOL 19.1002/ajmg.10261 region. More importantly, our investigation has defined the duplication of 5a34— ater as a distinet clinical phenotype. © 2002 Wiley-Liss, Ine. KEY WORDS: dup5q; cri du chat; molecu- lar cytogenetics; compara- tive genomic hybridization; fluorescence in situ hybri zation; dupSq/del 5p INTRODUCTION ‘The hallmark of the eri du chat syndrome is the dis- tinctive cat-like ery present in all patients. The clinical characteristies of the syndrome include severe psyeho- motor and mental rolardation, a round face, micro- cephaly, hypertelorism, micrognathia, hypotonia, and slow growth [Lejeune ef al., 1963; Niebuhr, 1978al, The chromosomal etiology of the syndrome, a deletion of a specific region (p15) on the short arm of chromosome 5, was first described by Lejeune and colleagues [1963] About 85% of eases are caused by de novo deletions (the ‘majority being paternal in origin [Overhauser et al, pg 1990); the remaining 10—15% of cases are familial and result from malsegregation of parental translocation chromosomes [Niebulr, 1978a]. Approximately 5% of the familial eases are caused when a parental inversion recombines to produce a gamete with partial monosomy of the short, arm and partial trisomy of the long arm (recombination aneusomy of a familial pericenivie inversion) [Niebubr, 1978a]. Although the size of the lost 5p material leading to the eri du chat. syndrome varies, all patients appear to have a deletion of 5p15.2 [Niebuhr, 1978b; Goodartet al., 1994; Overhauser et al 1994], The etiology of the characteristic cat-like ery is & ~ deletion of 5p16.8 just distal to the critieal region [Chureh et al., 1995; Gersh et al, 1995] More than two dozen patients have been described with partial trisomy for the long arm of chromosome 5 [albert et al., 1975; Bartsch-Sandhoff and Lierseh, 197; Zabel et al., 1978; Curry et al., 1979; Jones et al., 1979; Kessel and Pfeiffer, 1979; Rodewald et al., 1980;yp Jo Spuroddqearg ou, (q{T Us) JoqjOUr oy} WE g auIOS, rowiouyp Jo wols19au1 dunuanwed paouyyeq Afjuaedde us puw souyy ayy UL adoro apou [RuOW vw po[veaau sissjwuw owosouoay [eqUAIRA “CWT Ba) ada sosuey dgppexx'gr © pamoys puegosd oy) Woy soyfooyduts] poog [exoyditod Jo saipnys agouotINn siinsaa ¢vo "eHRIQ BTS “dog AuBewy paydéy) axeayos F{H] pur wos garg y,HOIstAo}AD © Mugen paenso}iod sum stesyouL aieun [Hiaiq_“wrr9ju9 ploysaya aya your ‘oqyoxd owos owoayp offs yoes Jo uoreUIGIOD a7 ON] PaaKap ‘camoys you vyUp) Sfeasogur souapyTo 29g 20) It gueaofor paroprsuo s|u0 aiais Sassor pure SUI [e661 e y9 atoueyy np} [vLdoseur fouIOSoULERED Jo (WO79|9p) ssof e jordsoqUT 07 pasn sem ggg> Jo ployssayy Jao] # O[ps “ToLs0]UUK eULOSOMOTE jo EEA Y aUyop 9} posn sem z'T< 30 proysamp sedan uy sequin Soo jwumosouanf aif) ja aqeys paouopeg au squasead -au 0°T Jo one Y “GT uD “eZ'T “OT “GL'0 ‘SO OUI 6 Jo] tH04)) Jo Sore qtasexax (HoT|AS SyINsAgy Aas) se]yotd }H0 ay UI Sout] ou, Spy Jad poutmgd oxo autosouubsys x8 YaRO Jo UoAds puy atmoseyTE TPA JO OT 48¥9] 38 40} (pexstioox) Fores SoUBDSAAONY au, “[BBBT “jp 30 Saa']) paquasop sIenoraoad se poutsojiod se SIsdqeUH HDD [esr “Te 99 wlisoyg] cyO ‘euBsuyINg ‘saqioqexoqey, 10490) PPLYse}eA Ut TaVE THA ¢'9 ‘quis Hurutepsionunos 44 payeprpany alow Susdayociny ue wormayRRUApY AWOSHMIO.NL “CAN “OTLAPOH COHEN adossoionut soudaeatony ya-IOKdoReT B BIO (ZV ‘uosong, “Samamoyoyd) PiouRe vApIA 90 pajo09 jyuyegoainary uw (yun parnydea 91a soveUt HD) SGT “Te fo A607 'FG6r “1 19 wuIOMONTEY] ssMorAord poquisep se pays pur pasedad aso% Saqaat [9 DO (Yo ‘oumesuyping ‘Sersoqeaoge] zoPaA) PlMIse}9aA, ‘ur aq THySur C'g UPI poureysxoyuNo9 o10% speoads aseyds yop “SuoNINAySUT S.tomnyoemMareM ayy 0} Surp.c9 oe poumopiod sta Hsia pur *(/T] ‘enous g.4ousoqy siséq Woy pasmbae elem sagard sueurojayqns bg puo dg pum (geSea'tguged) agoad yey np Ha ou, HSIa “12661 “90m pew canoagy sonbxuGDO) pap es ahem porsiogsed shh sytissnd amy pase purges ay) wos) pool [esaKdand jo s\ssjouw ofpotesons) sorpmg o1youasion. ‘SoUsIa Squazed ox UE douspaDd9e Ut Las jou axon puvgoud axp so sydwaRoqoyg “aswastp Hun] ae omya YUM qu9}sISU0 otoM SHUP}LOM Bun} ax} pu ‘ozs avay [euLIOU v poywAsUOMIAP eax Woy; “sopTyHOA oWwTEp pafwasa. eos (7.9) Aywasowoy pornduioa v pur ‘uoqTETUTEXD uo [ny a1oM saylaUAUOY JET "UD gzg SUH OF 10q pur 3y g'Z Sea 1YTTOM Loy ‘ae Jo syTUOU Is BW “dVdo_ pue UeSAxo woy pauom em ays puL ponoidiit 2urpaqy 104 “A108ns Joye yauour y “ampao -oad Jet} SULNOTO} ANdex YOU PIP WISN, [eIpswoLed 61 any, s]seyuacorpaeo.iad v parnba. yetp ping [eppsworied yo Uonupmunose powedxaun uoppns B zo} ofqexceUUeL Sem osimon aanjetedo-jsod Jop] “o3U Jo sU{]M0U Any 2B pouuopiod sess SA 404 Jo seas Teas 'Tuypoay 9101 “Seflostu so} yuoualinbad pa aouapuadap JV) 204 01 ang ‘uoisuayadcy Areuound queayyates Gah IwoaTs 09 mol} PUOHDOATPHA az 97 GSA “9H payBsysuoUAP Sydvatorpimooqoo yeaday age Woot uo SO} 0 otf Uh Suojyeamyes uadsxo yn sisound> padojonop ose ous ‘qny suysedoene Aq sHuypaay dog J0 Wow pastooos aes pu oun soa0 o8e, Aipuseasoul ataeaaq q2KY sO] ‘Sdviou} 160) puv uEOsIp Jo olds us Huypoey WOK 4Aqyeeo paun pad ajoudsyoey oq 0} panusyuan alg “UD -ewjawopul Jo uoRAsRETUIpE TUIAO||O) IHOpeAD FO] Ou see smuonp ayy, Uae sei sNsOLIAyTE sTyMp JUoqed 8 'uoRIppe Uf “s{loLo}sod popuonxe pur cunados reno -taquas ax9 30 woreda ay WE ug IHR (CSA) 499)0p Teidas swyouquan 98.9] 0 ayosapoul w payeaysuoap AqdesBorp.eoo\ypy -paureyqo ses yNsUOD jo. 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SRD IHD ip {s9 Jo Sosta 00g, uh 9u0 Tog Tuo Joy S1UNOINN pL ‘amma sf ¢ auiosoug Jo uojssnatr asyu99}4ad [erEAEy © Jo Awestauv uoReulqiucooy] yuoUiRes ywosoUOLL pie Dywosty Hupnsaa ayy UF 180] 20 PauTER auw yyy (soa ipouiesyp} SuoPar feuOsOUOsY ayfoods op Jo won Sodio 9 Jo wou # exe sayTeUEIOUGE atsIoUaK Jo quoyxo PUB aangeu au, [ELE “Te 49 WiOyyDsity) ndcjomoy TeuLIOU ay) Pu ewiOSOUEDIY|D WORRIOAT 2tf) Uaadtjog quouiae peptoau otf UTM eAM990 1240 Butssou J ‘eio}ous Tap este PRGA SoyoUER poor] coqun sqqewiosorsosp Aujsnpord oj sur JB aie suole “AU oLTankied Jo S4a1aE9 [BU ATTAEIOUSYAL NoIssnosia ‘soqyoad HO Puno pry satwosoutoay> Butumewar ayy Ty “(GT By) Jowb-ebe wog war Su0y ‘op jo ured ferjred w pu g'eTd-aayde wosy wie yzoys ary) 40 580] [Unyed w pasoys ¢ owosouIamp Jo ayyoad EY BUY, °G auiosoMOIyD yuLEIGUODI oY UE sTuFOdye=NG tpg auyos soxpary ©} poxygA sea YQ “aT HD Puegord orp ut Horror be peuruuey ayy Jo Woreardnp & ue uoysor dg peusunia} oy Jo S89] op payszswOUIp pu (CL-Hg) soxpouroyp at worsaauropsquaolaad ayy wLIyUOD 0} posh a1om by puv de soy aypode soqosd opoWolay, ‘SUOMOTUELMAl asoy) Jo auMieu ay) suTULIalep OT pauopiod sea sisdyeur FIST doupung "Wwawesuvwead [ewosouroayp jo 964} owtOs poreOMpUL MYA “(OL Hy) semosourosys snefojomoy om} oy} UO ou uy you sea ¢ omIOSoMTOIYD jo UATE WOYS oY UINIIA aqoud yey np uo axp Jo juomoET aarqyaL ayy, “OT SUL) Seuosoumo.yp aagyeatsop pur [eUIAON ay) 4304 UO SyeUIS polvaaan pur ‘gerde oy sou rorya “(gzsoq TSEC) aqoxd oypoodss,jvy9 np uo, [enrounos v Burn pamreprad azow saIpMs HSI “419 Fu otf JO ALA UL (AT BLD (eT 'Sebzeve eTHLOAUENN'OF ‘un? BuO, olf) UL ['Ggbg puw ULE yOYs au uF g-glde 0} paudisse Ayaanequey ox9M uorSssoauT [euta} Eu HSLd PUP HDD Aq pouyad edsyoudyg beampCase 4 Case 8 Corry etal, [1979] Case Case 2 Case Case 2 Deemer 11988), Case 1 ‘aa [1986 Seliosder eal. ‘Kumar [1987] Chern etal i962} Wittens etal [i998) ‘TABLE 1. Consistent Clinical Features Observed in Pationts With Pattial Trisomy of Distal bq” Present tal. a q syndrome and partial trisomy of 5q with specific FB Bes ry ie -44]8 chromosomal regions. We have confirmed that the 3 5 characteristic cry of the cri du chat syndrome is due to the deletion of the most distal part of the classic del 5 i 5p region. More importantly, we have narrowed down Bg Be sae 4/8 a critical region on the long arm of chromosome 5 2 5 z (Sq34— qter} that gives rise to a specific clinieal pheno- 2 typewhen duplicated. i q ea .8 , a ‘ACKNOWLEDGMENTS ga Rott tele . . o q ‘This work was supported in part by a grant from the © March of Dimes (FY00-408 to KED. BL is supported in Baio 5 part by @ National Institute of Child Health and Human, BP Boee ]h Berne ting an te HOME. 5 REFERENCES . a ‘Abuilo DN, Ahsunuddin AN, Mark HF. 2000. Distal 3 trisomy resulting Bg Ege. cae --s le ete ai te oceania ed 4 és i Genet 4502 90. = 4 ‘Baceichetti C, Lenzini B, Artifoni L, Caufin D, Marangoni P. 1988, Terai. ih din the nt tom Sones ohn Go SOS Boe ge 2 aa ae ge uaee Barak Sand, Uench 187, Parl apa 89 nome gb eR tt a ‘Dhenspi slaty ie sates veh eaten nets grt A BeShas gid ese Sa pty 3 ae i ot har deka ss oak , i ‘rea Sirs B. Won JT. Deora 0d ater JM, Beene FA, be ge 2 iss Pal ideo‘Puutaldantalonafsoenase sgt fof Ege. tee merle taker wn ces geyser ges 3 {ther anaes Hm Gout Bh : rome MG Lae UL 187 ural ed poetic math i st en ae a a ea 4 . 4 [Buy nal how fre ip oven 9 2 8 af-s1s- gale yout, Kino, Rose H, Maa, Gorda Rarhu R Ralonion a § 83 #28 "A Ghniaunsen & Landaloon C, Prfp 3 1600, Compurstve oosate 3 : mts init ata a sa cae i Chern Fol BB. Gx DM, 182. Cdn ned . i “Scab tevacalis ea penewtie mvenbu Scotee Cn Gea Es ge 3 Taw EE Beste 2 (et DM, Dots U, Nile I, Wasnt i, Nie B18 2 las ft eine tent srl da tae 3 NURSE Rmipteare And ise n & 3 Gurzy C9, Loughman WD, Francke U, Hall BD, Golbus MS, Derstine J, 2 a Epstein CJ. 1979. Partial trisomy for the distal long arm of chromasome BE pgss-+ ¢ p Bo mg++-+- gale Boren 34 lene te qa Anew chnially ocpalable Sperone g tu Cane 1tSt du Mane & Kalani OF, Ltr ¥ ger, ends B, Careers . “alr ns ia toe BE ee ces iettownth acer pan pee Coa gag 22 ‘B25 & neinet AC Hl, Lancer VF Senter" let, 2968 he Hl ‘Sut nigga sro: ape fob 18 re a i teach ach Di en os a4 a8 = nie 3 Wr Se J Tan H3, Rap AK 198, Anis xifding re § bgahis-r+eee+|¥ on ee ee ey S Be i Pryna JP. 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