2100 SERIES SPECTROPHOTOMETER USER’S MANUALTable of Contents General Information... Introduction... Working Principle............... Specifications... Unpacking Instructions... Installation... 2100 and 2100UV Spectrophotometer Operation Pan: Model 2100UV Operation Panel Model 2100 Operation Pane... Start-up Self Calibration... sce Changing Curette Holders... Basic Operation... ‘ Sample Preparation and Analysis. 4 Concentration Mode. WAS Factor Mode....... Output and Data Processing. Printer... FREDERIKSEN® Application Software. Maintenance.................. Lamp Replacement Wavelength Calibrat : Absorbance Accuracy Check. Stray Light Check..... 2100 and 2100UV Parts List.... Troubleshooting... Error Codes....... Attachment...General Information The spectrophotometer described in this manual is designed to be used by properly trained personnel in a suitable equipped laboratory. For the correct and safe use of this spectrophotometer it is essential that laboratory personnel follow generally accepted safe procedures in addition to the safety precautions called for in this manual. The inside of the power supply unit is a hazardous area and its cover should not be removed under any circumstances. ANY Servicing must be done by an authorized person. Some of the chemicals you use in the spectrophotometer may be corrosive, flammable, radioactive, toxic, and/or potentially infective. Care should be taken to follow the normal laboratory procedures for handling chemicals and samples. Please carefully read the Safety, Electrical, Warming, Performance and Radio Interference instructions below. Safety This spectrophotometer has been designed and tested in accordance with EN 61326-1: 1997 Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use standard (EMC Requirements). The spectrophotometer has been supplied in a safe condition. The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT, 1974. Read the following before installing and using the instrument and its accessories. Electrical Before switching on the spectrophotometer, make sure it is set to the voltage of ‘the local power supply (see Installation). The main plug shall be inserted in a socket provided with a protective earth contact. The protective action must not be negated by the use of an extension cord without a protective conductor. Warning Any interruption of the protective conductor inside or outside the spectrophotometer or disconnection of the protective earth terminal is likely to make the spectrophotometer dangerous. Intentional interruption is prohibited. Whenever itis likely that the protection has been impaired, the spectrophotometer shall be made inoperative and be secured against any unintended operation. NEVER touch or handle the power supply on the Model 2100UV due to the high 3voltage. The protection is likely to be impaired if, for example, the apparatus * Shows visible damage * Fails to perform the intended measurements * Has been subjected to prolonged storage under unfavorable conditions «Has been subjected to severe transport stresses, Performance Camry out performance checks with particular reference to wavelength and absorbance accuracy fo ensure that the spectrophotometer is working within its specification, especially wen making measurements of an important nature. Periormance checks are detailed in this manual. Radio Interference For compliance with the EMC standards referred to in the EC Declaration of Conformity, it is necessary that only shielded cables supplied by FREDERIKSEN® are used when connecting the instrument to computers and accessories. Introduction The FREDERIKSEN® Model's 2100 and 2100UV Spectrophotometers are single beam, general purpose instruments designed to meet the needs of the conventional laboratory. FREDERIKSEN® 2100 and 2100UV are ideal for various applications, such as: Clinical Chemistry, Biochemistry, Petrochemistry, Environmental Protection, Food and Beverage Labs, Water and Waste Water Labs and other fields of quality control. Featuring a digital display of photometric result, easy operation and wavelength fange of 325~1000 nm (Model 2100) or 200-1000 nm (Model 2100UV), FREDERIKSEN® 2100 and 2100UV are ideal for measurements in the visibl wavelength region of the electromagnetic spectrum (ultraviolet and visible for the Model 2100UV}. Working Principle The spectrophotometer consists of five parts: 1) Halogen/Deuterium Lamp to supply the light; 2) A Monochromator to isolate the wavelength of interest and eliminate ihe unwanted second order radiation: 3) A Sample Compartment to accommodate the sample solution; 4) A Detector fo receive and convert the transmitted light to an electrical signal: 5) A Digital Display to show absorbance or transmittance. Figure-1 illustrates the relationship between these parts.a OO OA UghtSouce —_Mongchromator Sample Compartment Detector Digital Display Figure-1 Block Diagram for the Spectrophotometer In your spectrophotometer, light from the lamp is focused on the entrance siit of the monochromator where the collimating mirror directs the beam onto the grating. The grating disperses the light beam fo produce the spectrum, a portion of which is focused on the exit slit of the monochromator by a collimating mirror. From here the beam is passed to a sample compartment through one of the filters, which helps to eliminate unwanted second order radiation from the diffraction grating. Upon leaving the sample compartment, the beam is passed to the silicon photodiode detector and causes the detector to produce an electrical signal that is displayed on the digital display. Specifications Table-1 lists the specification for both Model 2100 and 2100UV. Toble-1___ Specifications Model 2100 Model 2100UV Wavelength Range 325-1000 nm 200-1000 nm. | Spectral Bandpass Sam Wavelength Accuracy Better than 21nm_ Wavelength Repeatability Betfer than 1 nm. Stray Radiant Energy. 0.3% T at 340 and 400 nm. Photometric Range O-125%T -0.1~2.5 Abs 0~1999 C (0~1999 Factor) Photomeiric Accuracy £0,004 Abs at 0.5 Abs Power Requirements 115/230 V.+10%, 60/50 Hz switchable ‘Sample Holder 4poisiton Cuvette Holder Dimensions ‘470W x 400D x 140H {mm} Net Weight 10-kg (22 Ibs) 12 kg (27 Ibs) Uny Instruction: Carefully unpack the contents and check the materials against the following packing list fo ensure that you have received everything in good condition: Packing Listd Refer to Table-2 for the Packing List. Table-2 Packing List Description Quantity Model 2100 ‘Model 2100V | Spectrophotometer i 1 Dust Cover 1 1 Cuvette Set of 4, glass | Sel of 2, quartz; Set of 4, glass 4-position Cuvelte Holder T installed) 1 {installed} | (Manual) Power Cord i 1 User Manual 1 1 Installation 1. Affer carefully unpacking the contents, check the materials with the packing list above to ensure that you have received everything in good condition. 2. Place the instrument in a suitable location away from direct sunlight. In order to have the best performance from your instrument, keep it as far as possible from any strong magnetic or electrical fields or any electrical device that may generate high-frequency fields. Set the unit up in an area that is free of dust, corrosive gases and strong vibrations. 3. Remove any obstructions or materials that could hinder the flow of air under and around the insirument. 4. Select either 230V or 125V/115V on the voltage selector switch on the back of the 2100 and 2100UV shown in Figure-2, to match your local main voltage supply. 5. Tum on your FREDERIKSEN? 2100 and 2100UV and allow it to warm up for 15 minutes before taking any readings. Z ES) GD 5E@®) cm) tFigure Back of FREDERIKSEN® 2100 and 2100UV 2100 and 2100UV Ihotometer Operati | Model 2100UV Operation Panel Figure-3 shows the Model 2100UV and its Components: + Sample Compartment: Accommodate different cuvette holders. 4-position Cuvette Holder is preinstalled in the standard package * Power Indicator: Indicate whether power is on or off * Cuvette Holder Control Knob: Select the sample position of the 4-position Cuvette Holder by pulling out or pushing in * Operation Panel: has eleven components, see below for details. Figure-4 illustrated the detailed information of the Operation Panel: 1, Data Display Screen: Display the data of Transmittance, Absorbance, Concentration, and Factor modes. 2. Mode Indicator: The red LED light indicates the operation MODE. Allow the operator fo know the measurement mode currently in use (T-%Transmittance, A— Absorbance, C-Concentration, and F-Factor). 3, 0A/100%T Button: Blank the 2100/2100UV when biank reference solution is in the Sample Compartment. Every time a new wavelength is selected, press OA/100%T Button to confirm. It will automatically blank the 2100/2T00UV. 4. MODE Button: There are four modes. Tmode is fransmittance mode; A mode is absorbance mode; € mode is fo measure unknown sample concentration through @ standard solution; F mode is to measure unknown value with a previously determined factor, 5. PRINT Button: Press to send Data Display Screen and Wavelength Display Screen results to RS-232C port and printer. 6. Wavelength Display Screen: Display the curent/desired wavelength. 7. Wavelength Control Buttons: Allow user fo select the desired wavelength with the A and V [increase and decrease} buttons. 8. INC/DEC (Concentration/Factor Buttons) and Lamp Control Buttons: Allow user fo increase or decrease the aisplayed Concentration at C mode or Factor number at Fmode. At Tmode or A mode, press INC button will shutdown the halogen lamp (W}, press DEC mode will shutdown the deuterium lamp (D2). To furn on the lamps, Just press the INC/DEC button again. 9. ENT Button: First press at C mode enters reading on Data Display Screen. Other presses send displayed results to printer. If using FREDERIKSEN® Windows-based Application Software, press this button to communicate with the software {refer to 7the FREDERIKSEN® 2100 Series Spectrophotometer Software User's Manual Version $B-2.10 for details). if operating in F Mode, then press to enter factor. This causes the Mode to enter the factor number and change to C mode. 10. Visible Lamp Indicator (W): In the operating mode of Absorbance or Siransmittance (A or T}, pressing the INC buiton will tun off/on the lamp. 11. UV Lamp Indicator (D2): in the operating mode of Absorbance or %Iransmittance {A or), pressing the DEC bution will tum off/on the lamp. Since Deuterium lamp (D2) is an expensive item, it is recommended to tum off the) when not using ultraviolet light for measurement. Sample Conpartment Sample Holder Control Panel Power Indicator Figure-3 Model 2100UV Spectrophotometer6 7 8g 9 Figure-4 Operation Panel of Model 2100UV. Model 2100 Operation Panel The Model 2100 has the similar outlook as Mode! 2190UV. It also has the following components: + Sample Compartment: Accommodate different cuvette holders. 4-position Cuvette Holder is preinstalled in the standard package * Power Indicator: Indicate whether power Is on or off + Cuvette Holder Control Knob: Select the sample position of the 4-position Cuvette Holder by pulling out or pushing in «Operation Panel: has nine components, see below for details. Figure-S illustrated the detailed information of the Operation Panel: 1, Data Display Screen: Display the data of Transmittance, Absorbance, Concentration, and Factor modes. y Mode Indicator: The red LED light indicates the operation MODE. Allow the operator fo know the measurement mode currently in use (T-ZTransmittance, A~ Absorbance, C--Concentration, and F-Factor). 3. 0A/100%T Button: Blank the 2100/2100UV when blank reference solution is in the Sample Compartment. Every time a new wavelength is selected, press OA/100%T Button to confirm. It will automatically blank the 2100/2100UV. 4, MODE Button: There are four modes. T mode is transmittance made; A mode is absorbance mode; C mode is to measure unknown sample concentration through @ sfandard solution; F mode is to measure unknown value with a previously 9determined factor. . PRINT Button: Press fo send Data Display Screen and Wavelength Display Screen results to RS-232C port and printer. » Wavelength Display Screen: Display the current/desired wavelength. . Wavelength Control Buttons: Allow user to select the desired wavelength with the A and V {increase and decrease) buttons. a Noe 2 INC/DEC (Concentration/Factor Buttons): Allow user fo increase or decrease the displayed Concentration at € mode or Factor number at F mode. . ENT Button: First press at C mode enters reading on Data Display Screen. Other presses send displayed results to printer. If using FREDERIKSEN® Windows-based Application Software, press this button to communicate with the software {refer to the FREDERIKSEN® 2100 Series Spectrophotometer Software User's Manual Version ‘$B-2.10 for details). If operating in F Mode, then press to enter factor. This causes the Mode to enter the factor number and change to C mode. °° Figure-5 Operation Panel of Model 2100 tart. bration The Model 2100 and 2100UV have self-calibration programs that begin when the instrument is powered on. The following are the steps taken automatically each 10time the instrument is switched on. Each step is displayed on the Wavelength Display Screen. Figure-6 to Figure 12 and Table-3 show the detailed information of the Start-up Self-Callbration: P1 Process, P2 Process, P3 Process, P4 Process, Show FREDERIKSEN Process, PC CONN Process, and Normal Start-up Screen. Figure-6 P 1 Process Table-3 Start-up Self-Calibration Display 2100 2100UV. PI ‘Check the electronic components | Check electronic components, and positions secondary filters initiates lamp selection and positions secondary filters P2 Monechromator locates starting ‘Monochromator locates starting position position Monochromator locate “0” order light and measure initial light energy ‘Monochromator locate “0” order light, and measure initial light energy _| ‘* Monochromator go to 546 nm ‘= Instrument sets O%T * Initiates RS-232C port. Show "PC-CONN" + Set 100% (blank reference] * Monochromator goes fo 546 nm ‘Instrument sets OT. * Initiates RS-232C port. Show “PC-CONN" + Set 10021 (blank reference}Figure-7 P 2 Process Figure-8 P 3 Process Figure-9 P 4 Process Figure-10 Show FREDERIKSEN ProcessFigure-11 PC CONN Process Figure-12 Normal Start-up Screen Ch Cuvette Hol There are seven different Cuvelte Holders (Sample Holders) (refer to Figure-13 and Figure-14, from left to right): ¢ $-2100-101P: Test Tube Holder for 8~20 mm diameter test tubes. Includes Universal Base, one holder, and Top Hat for up to 150 mm tall test tubes * $-2100-102P: Rectangular Long Path Cell Package for cells up to 100 mm. pathlength. Includes Universal Base and one holder ‘* $-2100-103P: Single 10 mm Cuvette Holder Package. Includes Universal Base and one holder + $-2100-104P: Cylindrical Cuvette Holder Package for cells up to 100 mm pathlength. (22mm diameter). Includes Universal Base and one holder * $-2100-105P; Water-Jacketed Single Cuvette Holder Package for 10 mm cuvettes for temperatures from 0~95°C. Includes Holder, Universal Base, and Flowthru Panel (Water Bath Required) * $-2100-106P: Micro-Cuvette Holder Package for use with 10 mm microcuvettes. 13Includes adjustable x-y base and holder * $-2100-109P: Peltier Flowcell Package for 25, 30 and 37°C, Includes p/n $-2100- 109, peristaltic temperature/pump/flow-thru controller, p/n $-2100- 107A, Peltier Thermal Electric Cuvette Holder and peltier/fiow-thru Refer to the Attachment in this Manual for the detailed Installation Guide. The only tool needed is a standard Philips head screwdriver. 4 ~ 1 ) Figure-13 Cuvette Holder 2100-101P to 2100-104P S| , Figure-14 Cuvette Holder $-2100-105P, 106P and 109P Basic Operation > Three Basic Operations: Sample Preparation and Analysis, Concentration Mode—C, and Factor Mode are shown in this section. Sample Preparation and Analysis A. — Spectrophotometer Warm-up and %T Check fit Turn on the spectrophotometer by turning on the Power Switch (IO). Allow 15 minutes for the instrument to warm up. Operational Note: The instrument performs four self-calibration checks every time the power is turned on. For details of the self-calibration and the error codes associated with this, please see the Error Codes section of this manual. 2 Select either the T (Transmittance Mode) or A (Absorbance Mode) by 14ap NO PA VW. 12. pressing the MODE button until the red light for T or Ais on. Select the desired wavelength by pressing the appropriate Wavelength Control buttons A and V. At any time if the wavelength is changed, the Data Display Screen will display “BLA”. This serves as a reminder that a reference is necessary with the change of the wavelength and you need to Push 0A/100%T Button to blank. Sample Preparation Make a blank reference solution by filing a clean cuvette (or test tube) half full with distilled or de-ionized water or other specified solvent. Wipe the cuvette with tissue to remove the fingerprints and droplets of liquid. Insert the blank cuvette into one cell of the 4-position Cuvette Holder. Push or pull the Cuvette Holder Control Knob so that the cuvette is in the light path). Close the cover. Set 0.000A or 100%T with the 0A/100%T Button. Remove the blank cuvette if you are testing more than three samples. Set it aside in the case that you may need to adjust the 04/100%T button later (Le. change the wavelength). Sample Analysis Rinse a second cuvette with a small amount of the sample solution to be tested. Fil the cuvette half full and wipe it. Put the sample cuvette in the Sample Compartment. Close the cover. Read the T or A from the Data Display Screen. If you cre reading more than one cuvette, be sure to carefully move the stage to the next position by pulling on the Cuvette Holder Control Knob until you feel the holder “click” into place. Be sure to make note of the test results (or print) for each sample, Remove the sample cuvette(s). Ifyou are to test the same sample at other wavelengths, repeat step 3 f0 10 for each wavelength. For each new sample you analyze, repeat step 2 to 11. Concentration Mode C’is used for determining the concentration of unknown samples. NOTE: This method should only be used when the relationship between Absorbance and Concentration is known to be linear. The concentration of the Standard solution used to calibrate the instrument should be higher than the most concentrated sample. 1, Select the desired wavelength by pressing Wavelength Control buttons A and Viuring the wavelength control knob. 2. Using the MODE button, select A mode. 3. Insert the cuvette containing the blank solution. 15Set 0.000 with the 0A/100%T bution. . Using the MODE button, select C mode. . Insert a cuvette containing a standard solution of known concentration in the first position of the Sample Compartment and set the Data Display Screen to be the value of the standard by using the INC and DEC buttons. 7. Press the ENT button. aoe NOTE: If the reading changes, the factor required is too high {e. >1999) to be displayed. In this case, divide the concentration by 10; re-select the € mode by successive presses on the MODE button, cycling through the FT, and A modes, and follow step 2 above to set the concentration of the standard to the reduced value. 8. With the standard concentration set, determine the concentration values of samples with unknown concentration by inserting the sample cuvette into the Sample Compartment and reading the value direct from the Data Display Screen. 9. To read the value of the multiplier used to convert Abs to Concentration, after measuring all the samples, change the mode to F and read the multiplier from Data Display Screen. Keep a record of this value for future use. Operational Note: if the mode switch Is changed to read For A, the Concentration C reading is “frozen”, and cannot be changed. This requires the operator fo re- start at step 1. Factor Mode This is a special mode for measuring concentration values of unknown samples using a previously determined factor to convert absorbance readings to concentration. 1. After setting the wavelength, and setting zero Abs on the blank solution, using the MODE button, select F mode. 2. Insert a cuvette containing a sample. 3. Using the INC and DEC buttons, set the Dota Display Scre valve of the multiplier. 4, Press the ENT button. The spectrophotometer switches to the C mode. mn to the desired Operational Note: if the Concentration of the sample is too high to be displayed, the instrument will not switch to © mode when the ENT button is pressed. Dilute the sample and multiply the concentration reading by the dilution factor to obtain the original sample concentration. IF dilution is impossible or causes other problems, you may divide the factor value by "10" or "100" and follow Step 1 to 4 to enter the “new” factor value. You need to calculate the sample concentration by multiplying readout with the multiple "10" or "100". 165. Read the concentration value of the sample direct from Data Display Screen. 6 Insert a cuvette containing the next sample and read the result. Repeat until all samples have been measured. Operational Note: If the MODE switch is changed to A or T, then the concentration reading is “frozen”. This requires the operator to re-start at step 1. Qutput and Data Processing Printer Model 2100 and 2100UV have a RS232C Interface connector that can be connected to any RS232 printers (FREDERIKSEN® provides RS232 printer, p/n: -1100- 206, as an optional accessory). It requires a 9-pin, null modem connection cable. The RS232 Printer Setting should be as follows: Baud Rate: 9600bps Parity: None Data Bits: 8 Stop Bit: 1 FREDERIKSEN® Application Software The FREDERIKSEN® Application Software--2100 is Windows-based software designed to operate with FREDERIKSEN® Spectrophotometer Model 2100 and 2100UV. Model 2100 and 2100UV uses its RS232C Interface connector to connect with a PC. The software runs on a PC with Windows® 95/98/Me/NI/2000/XP operating system installed. The Software offers two additional analytical methods: Standard Curve and Absorbance vs. Time Kinetics. It performs the following methods for analysis: * Absorbance/fransmittance/Concentration: measure the Absorbance, seTransmittance, Concentration/Standard, or Concentration/Factor at a single wavelength within the range of 325~1000 nm (200~1000 nm for 2100UV). * Standard Curve: create a calibration curve (choice of 4 curve fits} with up to 8 standard solutions at a single wavelength to determine concentrations of unknown samples. Absorbance vs. Time Kinetics: measure a sample's absorbance change over a selected period of time, store the test results in data table, and display the results graphically. Refer to the FREDERIKSEN® 2100 Series Spectrophotometer Software User's Manual Version $B-2.10 for detail. MaintenanceLamp Replacement Replace Tungsten-Halogen Lamp . Tum off and unplug the instrument. . Remove the four screws on the sides of the spectrophotometer. . Remove the Cuvette Holder Control Knob by unscrewing the rod counterclockwise. . Remove the cover of the instrument very carefully and place it in front of the instrument. eY-p » HINT: Lift up about 3~4 inches, and once the instrument has cleared the backside grill plate, lift the back of the cover up towards the front. The front of the instrument will act as a “hinge”. BE SURE TO NOT PULL PANEL WIRING LOOSE! 5. Unplug and remove the lamp from ceramic base (the white connector). Insert the new lamp; pushing it in as far as it will go. Part Number: $-2100-515 (6V 10W G4 type) CAUTION: DO NOT HANDLE THE LAMP WITH BARE FINGERS. USE TISSUE OR CLOTH WHEN HANDLING LAMP. 6. Turn on the instrument. Set the wavelength at 340 nm, insert an empty cuvette, and blank the instrument. If the energy is low, adjust the lamp by “pulling” or “pushing” it so that the light beam is focused on the entrance slot of the monochromator. Since the lamp socket is pre-aligned, there will be minimum, if ‘any, adjustment required. . Reinstall the instrument cover by positioning the front of the cover first and then sliding the back of the cover over the backside grill plate. Be sure alll wires from the front panel are not pinched in the process. N Note: The latest version of Model 2100/2100UV has a lamp access door at the back of the instrument. If your Model 2100/2100UV has the lamp access door, you do nof need to open the cover. Just open this door to replace the lamp. 8. Reinstall the four screws and the Cuvette Holder Control Knob. B. Replace Deuterlum Lamp WARINING: Wear UV protection Glasses before changing the Deuterium Lamp! 1. Tum off and unplug the instrument (VERY IMPORTANT: HIGH VOLTAGE). 2. Remove the four screws on the sides of the spectrophotometer. 3. Remove the Cuvette Holder Contro! Knob by unscrewing the rod counterclockwise. 18,4, Remove the cover of the instrument very carefully and place it in front of the instrument. HINT: Lift up about 3~4 inches, and once the instrument has cleared the backside grill plate, lift the back of the cover up towards the front. The front of the instrument will act as a “hinge”. BE SURE TO NOT PULL PANEL WIRING LOOSE! 5. Remove the metal plate covering the Deuterium lamp by removing the two screws from the rear of the plate (make a note of the wire color and their matching connectors). 6. Remove the lamp by disconnecting the 3-wire connector and pulling straight up on the lamp socket. DO NOT PULL on the lamp itself. This may cause the lamp to break. 7, Replace the pre-aligned lamp with a lamp provided by FREDERIKSEN® or an authorized FREDERIKSEN® Service Provider (Call 1-800-588-9776 for details). This comes pre-assembled with lamp socket. 8. Reconnect the wire connector and be sure the lamp and lamp socket is securely in place (make sure the wire connection orientation is the same as step 5.) 9. Tum on the instrument. After the Start-up Self Calibration, select 300 nm and press 0A/100%T Button. Check to make sure that the light beam is focused on the entrance si of the monochromator. Ifit is not, adjust the mounting screws on the Deuterium lamp holder to align the lamp. Note: The latest version of Mode! 2100/2100UV has a lamp access door at the top of the instrument. If your Model 2100/2100UV has the lamp access door, you do not need fo open the cover. Just open this door to replace the lamp. CAUTION: THE LAMP MAY BE HOT! TAKE PRECAUTIONS TO PREVENT POSSIBLE BURNS. Wavelength Calibration Check Normally the FREDERIKSEN® 2100 Series spectrophotometer retains its wavelength calibration indefinitely. However if the instrument receives a severe shock or is abused, use the following methods to check wavelength calibration. Please note that this test requires the FREDERIKSEN® Didymium filter, p/n $-2100-116, or the Holmium Oxide filter, p/n $-2100-115. In the filter method, the didymium filter has two distinct absorbance peaks at 529 nim and 807 nm. The Holmium filter has a distinct peak at 361 nm. When the instrument is calibrated properly you will find minimum Transmittance (maximum. Absorbance) at the range + 2nm from these peaks. Note that the specific Transmittance values are not important as you are only looking for the wavelength where the minimum Transmittance (maximum Absorbance) occurs. 19Note: if you calibration filter has a certified peak/valiey curve attached, please use the peaks on the curve to verify the instrument. Holmium Oxide Filter Method - Tum instrument on and allow it to warm up for 15 minutes. 2. Select the A mode. 3. Set the wavelength to 350 nm. 4. Make sure the cuvette holder is empty and place it in the Sample Compartment. Close the lid. . Set zero Absorbance by pressing the 0A/100%T bution. Wait a few seconds while the display flashes 'BLA--'. The reading on the Data Display Screen should be 0.0004. If not, repeat step 5. . Remove the cuvette holder and insert the Holmium filter into it. Place it in the Sample Compartment and close the lid. . Record the Absorbance reading on the Data Display Screen. .. Advance the wavelength setting by 1 nm and repeat step 4 fo 7. . Repeat step 8 until the wavelength setting reaches 370 nm. 0. Look for the maximum absorbance reading obtained, and this should be found between 359 and 363 nm. The wavelength accuracy of the 2100 is + 2nm. a o = ON Didymium Fiter Mathod « Set the Wavelength to 800 nm. 2. Makeaue the cuvette holder is empty and place it in the Sample Compartment. Close the lid. 3. Set zero Abs by pressing the 0A/100%T button. Wait a few seconds while the display flashes ‘BLA--'. The Data Display Screen should then be 0.000A. If not, repeat step 3. |. Remove the cuvette holder and insert the Didymium filter into it. Place it in the Sample Compartment and close the lid. . Record the Absorbance reading on the Data Display Screen. . Advance the wavelength setting by Inm and repeat step 2 to 5. . Repeat step 6 until the wavelength setting reaches 815 nm. . Look for the maximum absorbance reading obtained, and this should be found between 805 and 809 nm. The wavelength accuracy of the 2100 is # 2nm. 9. Ifa "middie" wavelength check is desired, set the wavelength to 522 nm {optional) 10.Make sure the cuvette holder is empty and place it in the Sample Compartment. Close the lid. 11. Set zero Abs by pressing the 0A/100%T button. Wall a few seconds while the display flashes 'BLA’. The reading should then be 0.000A. If not repeat step 11. 12.Remove the cuvette holder and insert the Didymium filter into it. Place it in the Sample Compartment and close the lid. 13.Record the absorbance reading on the Data Display Screen. 14. Advance the wavelength setting by Inm and repeat step 10 to 13. 15.Repeat step 14 until the wavelength setting reaches 536 nm. Again, look for the 20 - PNAHmaximum absorbance reading. It should be between 527 and 531 nm. Absorbance Accuracy Check Specification: + 0.5% at 1A, + 1% at 2A. The absorbance accuracy should be checked against a set of neutral density filters accurately calibrated to the NIST standards, Contact your FREDERIKSEN® representative for more information (1-800-588-9776). An alternative method using potassium dichromate is described below. Due to the many factors that might affect the results (i.e. temperature, bandpass, weighing and diluting errors), this method is tess accurate and should only be used as a guide. Reference: Johnson E A Potassium Dichromate as an absorbance standard PSG Bulletin 1967, No. 17, page 505 1 Make up N/100 sulfuric acid as the solvent and use part of it to make a solution containing 120 + 0.5 mg/liter of potassium dichromate. fae Wash out a square cuvette with solvent, and fill with solvent. 3. Put the cuvette in the adapter into the Sample Compartment and close the lid. Set the wavelength to 350 nm. Set the MODE button to A. Set the reading on the Data Display Screen to 0.000A using the 0A/100%T button. 7. Empty the cuvette. Wash out with dichromate solution, and fill with dichromate solution. 8. Put the cuvette in the adapter into the Sample Compartment and close the lid. i Read the absorbance of the standard from the Data Display Screen. The value should be 1.288 + 0.02 A. Refer fo the notes above when interpreting the result. sae Stray Light Check Specification: Less than 0.3%T at 340 nm by ASTM E 387 A good indication as to whether the stray light level is within specification may be obtained as follows: 1, Set the wavelength to 340 nm. 2. Set the MODE bution to T. 3. With the Sample Compartment empty, close the lid and press the 0A/100%T 21button to set the display to 100.0%. Hold the MODE key for at least 5s. This will set the 0.00%1. This may take a few minutes. Prepare a solution containing 50 gm/L of sodium nitrite (NaNOz) in distilled water and fill a square cuvette with this solution. Insert the cuvette into the Sample Compartment, and close the lid. The Data Display Screen display should read < 0.321. If the reading obtained in step 4 is greater than 0.00, it should be subtracted from the displayed Data Display Screen to give the correct reading for the stray light value. 2100 and 2100UV Parts List Table-4 2100 an d 2100UV Parts List Catalog # Description '$-2100 FREDERIKSEN® Model 2100 Spectrophotometer 5 nm Bandpass Wavelength range: 325~1000 nm, automatic wavelength change, Voltage preset of ov ‘Complete with 4position Cuvetle Holder, 4 maiched Opiical Glass Cuvettes RS-232C Port, Dust Cover, User Manual S-2100-E ‘Some as Model 2100 but preset at 220 V 'S-2100UV FREDERIKSEN* Model 2100UV Spectrophotometer 5 nm Bandpass Wavelength range: 200-1000 nm. automatic wavelength change, Vollage preset at Lov ‘Complete with 4-posiion Cuvelfe Holder, 4 matched Optical Glass Cuvettes, 2 Quartz Cuvettes RS-232C Port, Dust Gover, User Manual 'S:2100UV-E | Same as Model 2100UV but preset at 220 V Software, ‘S-2100-401 | FREDERIKSEN® Application Sofiware for PC's, Window's 959/98 or above Required. Programs include Standard Curve, Abs. /%1/Conc.,.and Abs.vs.Time. Includes $-2100- 226 serial cable ‘Accessories S-2100-101P | Test Tube Holder Package for & fo 20 mm diameter test fubes. Includes Universal Base, ‘one holder, and Top Hat for up to 150 mm fall fest fubes $-2100-102P | Rectangular Long Path Cuvette Holder Package for cells up 10 100 mm pathiengin. Includes Universal Base and one holder '$-2100-103P | Single 10 mm Cuveite Holder Package. Includes Universal Base & one holder ‘$2100-104P | Cyindlical Cuveife Holder Package for cells up fo 100mm pathlength. (22 mm diameter). Includes Universal Base and one holder $-2100-105F | Water-Jacketed Single Cuvelfie Holder Package for 10 mm cuveltes for Temperatures from 0-95°C. Includes Holder, Universal Base, and Flowthru Panel (Water Bath Required) $-2100-106P | Micro-Cuvelte Holder Package for use with 10 mm microcuvelies. includes adjustable xy base and single Cuveite Holder for 10 mm square cuvette $-2100-107F | Pellier Package for continuous temperalure control from 15 to 40°C. includes p/n S- 2100-107A, peltier thermal electric 10 mm Cuvette Holder and p/n $-2100-1078 peltier controller unit 22$-2100-108P | Fiow-thru Package for continuous temperature contol from 15 to 40°C, Includes p/n $- 2100-109 Flow-thry controller and p/n S-2100-1088 flow-thru panel (requires flowcell and tubing) S200 05? ~ Peter oper Package for Tow Trough and aninuous fampsrature conal fom TST 40°C, Includes p/n $-2100-109, peristaltic/flow-thru controller and p/n $-2100-107A, Peltier Cuvette Holder with panel (requires flowcell and tubing} $-2100-101 | Test Tube Holder without base (Requres Universal Base) $:2100-102 | Rectangular Long Path Cuvette Holder without base (requires universal base] $:2100-103 [Single 10 mm Cuvete Holder without base (requires universal base) $:2100-104 [ Cylingrical Cuvette Holder without base [Requires Universal Base) ‘2100-105 Water-Jacketed Single Cuvette Holder with flow-ihru panel but without base (Requires Universal Base, Water Bath) ‘2100-107 __| Peltier Thermal Electric 10 mm Guvelte Holder with panel 2100-1078 | Peltier controller unit for controling temperature from 15 to 4°. S-2100-1084 | Flow-thru panel for use with ambient flowcell system requires flowcel, fubing and p/n S- 2100-109 flow-thru/peltier controller 52100-1097 | Peristatic Fump, Peliler Thermal Température, and low-thny Controller Only 2100 and 2100UV Parts List continued Catalog # Description ‘Accessories 2100-111 | Manual 4-posilion Cuvetfe Holder included with 2100 and 21000V) $-2100-112__| Universal Base. Accommodates up to 2 same or different type of holders 2100-113 | Top Hat for test tubes with height of 75~150 mm $-2100-114 [Spacers for 5 mm Cuvettes $-2100-115 | Holmium Oxide Filter $-2100-116 | Didymium Fitter $-2100-120 | Haake Mode! DC 10-83 water bath for temperatures from 25~100°C. For use with S- 2100-105 $-2100-121 Haake Mode! DC10-K10 water bath for temperature ranges of -10~100°C. For use. with $-2100-105 Ouiput Device $-1100-206 _[ Printer (requires $-1100-207 priner cable) S-1100-207 [Printer Cable [Male 25-pin to Female 9-pin, Null Modem) 2100-226 [ RS-232C Cable (9-pin to 9-pin Female/Female, Null Mociem) Glassware '$-90-301 ‘Test Tube Cuvettes, 10 mm Diameter, 2 pes $-90-302P-100 | Disposable Cuvettes. Polystyrene, 10 mm Pathlengih, 100 pcs $-90-302P-500 | Disposable Cuvettes. Polystyrene, 10 mm Pathlengih, 500 pes $-90-305P__| Semi-micro Disposable Cuvettes, Polystyrene, 4 mm window, 500 pcs $-90-304G | Square Cuvettes, Optical Glass, Set of Two $-90-309Q_| Square Cuvettes, Quartz, 10 mm, Set of wo '$-90-208-50G | Cyindral cell, 50 mm pathlength $-90-326G_| Rectangular cell, Optical glass, 50mm pathlengih, with stopper S-90-344FG | Flowcell, Optical gloss, 4x12 mm window, 0.48 ml $.90-340FQ | Flowcell, Quartz. 4x12 mm window, 0.48 mi $-90-350G | Square cuvettes, opfical Glass, 4 mm width Omi $-90-351Q | Square cuvettes, Quartz, 4 mm width, 1.0 mi 5-90-3520 __| Square cuvettes, Quartz, 4 mm width, with stopper, 0.5m S:90-353G | Square cuvettes, Optical glass, 2mm width, 0.5 ml $-90-354@ | Square cuvettes, Quartz. 2mm width, 0.5 ml 23$-90-355Q | Square cuveltes, Quartz, 2mm width, with stopper, 0.5 mi $-90-358Q "| Square cuvettes, Quartz, 2x5 mm window, 0.100 ml $-90-359Q__| Square cuvettes, Quartz, 2x2.5 mm window, 0.0 50 mi ‘Misc. and Replacement items '$-1100-508 __| Printer Paper, Package of '$-2100-510 | User ‘Manual $-2100-511 | Dust ‘Cover $-2100-515 | Tungsten-Halogen lamp for 2100 and 2100UV, Package of 2 (6V 1OW G4 type] $-2100-525 | Deuterium lamp for 2100UV $-2100-535 | Fuse, 3A, quantity 1 (size 5 x 20) Troubl oti: Table-5 _ Troubleshooting PROBLEM Possible Cause Solution Instrument Power cord not connected fo outlet_| Plug instrument in Inoperative | Dead Power outlet Change fo a aifferent (Power Wrong voltage setting outlet Indicator has [intemal fuse blown or defective Call an authorized service no light) electronic component engineer Inskument No cuvette in the Sample Cuvette adapter must be cannot set Compartment in Sample Compartment 100%T (0.000A) to open sample holder shutter light beam blocked: * Holder misaligned © Shutter ‘Check sample holder Lamp is old or defective Replace lamp Lamp is off alignment Refer to iamp replacement instructions in this manual Defective electronic component _| Call an authorized service engineer %1 cannot be Sample holder Remove cuvette holder set fo 00.0%T ortest tube Sample holder shutter ‘May be stuck open Close shutter Defective electronic component _| Callan authorized service engineer Incorrect Bubbles oF parficies in solution Check sample 24Transmittance to Absorbance correlation preparation and analytical procedure Defective electronic component Callan authorized service engineer Troubleshooting continued PROBLEM Possible Cause Solution Digital Display does not change regardless of sample concentration Concentration reading “frozen” MODE switch has been changed from C to F, T or A and back to C Restart measurement procedure Wrong wavelength setting ‘Check sample procedure and wavelength setting Insufficient sample volume Fill cuvette with more samples Stray sample preparation vapors. Prepare the sample away from the instrument. Use proper ventilation Bubbles or particles in solution Check sample preparation and analytical procedure Defective electronic component or loose wiring. Call. an authorized service engineer Instrument drift and nolse No sufficient warm up time Significant temperature change Lamp not adjusted properly ‘Check lamp has been properly installed or has moved during transit Refer to lamp replacement instructions in this manual Lamp old or defective Replace with a new lomp 25Sample Holder Misaligned Refer fo lamp replacement instructions in this manual Unstable power supply Defective or dirty detector or defective electronic component Call an authorized service engineerTroubleshooting continued PROBLEM Possible Cause Solution Incorrect Insufficient sample volume __| Fill cuvette with more samples readings Wrong wavelength setting | Check analytical procedure obtained Failed fo blank (04/100%T) | and wavelength setting Falled to set O%T Check wavelength accuracy according to procedure in this manual Stray sample preparation Prepare sample away from vapors instrument. Use proper ventilation Bubbles or parlicles in solution | Check sample preparation and analytical procedure Instrument out of electronic | Call an authorized service calibration engineer Emror Codes The Error Codes are the classification for erors detected automatically by the instrument, Each code represents different errors that occur during the self-calibration or during ‘operation. The Error Codes are displayed on the Wavelength Display Screen and are defined as Table-5. Table-6 __Error Codes Error Code Definttion/Function Causes/Solutions ERR OT Instrument unable to inifialize the Check power supply to see iF lamp (2100UV) or secondary fiter proper voltage is selected (2100) Open cover io instrument and check CPU board connections Lamp-change-over motor or Jamp initialization switch matfunctioning (2100UV) Filter-criven motor malfunctioning (2100) Call authorized service engineer ERR 02 ‘Monochromator unable fo locate starting position {2100}; or initialize secondary fier will not initial (2100uv) ‘Monochromator contact switch or grating-driven motor is malfunctioning (2100) Fiter-driven motor malfunctioning (2100) Call authorized service engineer 27Enor Codes Continued Enor Code Definition/Function Causes/Solutions ERR 03 Insirument unable to locate “0” Monochromator contact switch or ‘order light { 2100 and 2100UV}; or | _grating-driven motor is monochromator unable to malfunctioning (2100UV) locate starting position (2100UV) Check tungsten-halogen lamp. Replace lamp if necessary (both) Monochromator contact switch loose or mis-positioning (both} Check deuterium lamp (2100UV}— WEAR UV-protective eyewear Tungsten-halogen or deuterium Check lamps by opening cover as ‘amp is misaligned or no longer instructed in this manual (See functioning Maintenance) Replace lamps if necessary Contact an authorized service engineer ERR 04 instrument is unable to locate ‘Check sample compartment and 54énm and automatically set remove cuvette or any object O%T and 04/100%T blocking light path Place the 4-cell sample holder in the proper position Contact an authorized service engineer Attachment Refer the following attachments for the Accessory Installation Guide: * Universal Holder and Accessory installation Guide (2100-101P to 2100-104P) + Water-Jacketed Single Cuvette Accessory Installation Guide (2100-105P) * Micro-Cuvette Holder Package Accessory Installation Guide (2100-106P) * Peltier/Flowcell Accessory Installation Guide (2100-109P) 28Step | Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Universal Holder and Accessory Installation Guide {S-2100-101P to $-2100-104P) Remove all items from the accessory package and make sure that they are complete (refer to Figure-13 for the $-2100-101P fo S-2100-104P): A Universal Platform Ipc The specified holder Ipc Insiallation Guide Ipc The only tool needed is a standard Philips head screwdriver. Remove the 4-position Cuvette Holder: Open the Sample Compartment cover and remove any cuvettes or any breakable items. Push in the Cuvette Holder Control Knob (black knob) all the way back (First position is into the light beam). Unscrew the Cuvette Holder rod by fuming the knob counterclockwise until removed from holder. Gently slide the guide rod out of the Sample Compartment. Looking over the Sample Compartment, remove the front screw (Figure-15). eBid toe Figure-15 Remove Front Screw To remove the back screw, place your hand ON TOF of the 4-position Cuvette Holder and gently slide towards the front. The back screw is then exposed and can be removed. Insert Universal Holder by repeating Step 4 to 6 in reverses. Screw in your holder to any of the open four positions on the holder. NOTE: If a second holder is purchased (i.e. to make into a 2-position Cuvette Holder), Place the second holder in a position so one position is skioped. For example, if the first holder isin position one, and then the second must be in either position 3 or 4 of the Universal Holder. (Two same type or different types of holders can be installed on the universal platform). 29Water-Jacketed Single Cuvette Accessory Installation Guide (S-2100-105P) Step 1 Remove all items from the accessory package made sure that they are complete (refer to Figure-14 for $-2100-105P): > A Universal Platform Ipc Water-Jacketed Holder Ipc Flow-thru Pane! Ipc Installation Guide Ipc Step 2 The only tool needed is a standard Phillps head screwdriver. Step 3 Remove the 4-position Cuvette Holder: Open the Sample Compartment cover and remove any cuvettes or any breakable items. Push-in the Cuvette Holder Control Knob (black knob) all the way back (First position Cuvette Holder is into the light path). Step 4 Unscrew the Cuvette Holder rod by tuming the knob counterclockwise until removed from holder. Gently siide the guide rod out of the Sample ‘Compartment. Step 5 Looking over the Sample Compartment, remove the front screw (Figure-15). Step 6 To remove the back screw, place your hand ON TOP of the 4-position Cuvetie Holder and gently slide towards the front. The back screw is then exposed and can be removed. Step 7 Insert Universal Holder by repeating Step 4 to 6 in reverses. Step 8 Screw in the Water-Jacketed Holder to the fist (front) position of the Universal Holder (platform). Thisis to prevent stretching the tubing. + Steps Remove the front panel by sliding the pane! up and out (Figure-16). Removable Front Panel Front View Figure-16 Removable Front Panel Front View Step 10 Insert Front Panel for Water-Jacketed Cuvette in place of removed panel. 30step 11 Step 12 Connect Water bath to the front panel tubing connectors (Figure-17). Front Pana for ‘WoterJecketed Cuvalie Accetsery th Eerie Figure-I7__ Front Panel for Water-Jacketed Cuvette Accessory Set desired temperature on water bath (see instructions of water bath manufacturer}. 31Step 1 Step 2 Step 3 Step 4 Step 5: Step 6: Step 7: Micro-Cuvette Holder Package Accessory Installation Guide for (S-2100-106P) Remove all items from the accessory package made sure that they are complete (refer to Figure-14 for the $-2100-106P): = A Cuvette Holder with X-Y adjustable bas Tpc Installation Guide Ipc The only tool needed is a standard Philips head screwdriver. Remove the 4-position Cuvette Holder: Open the Sample Compartment cover and remove any cuveites or any breakable items. Push-in the Cuvette Holder Control Knob all the way back (First position Cuvette Holder is into the light path). Unscrew the Cuvette Holder rod by tuming the knob counterclockwise until removed from holder. Gently slide the guide rod out of the Sample Compartment. Looking over the Sample Compartment, remove the front screw (Figure-15). To remove the back screw, place your hand ON TOP of the 4-position Cuvette Holder and gently slide towards the front. The back screw is then exposed and can be removed. Place the x-y adjustable base into the Sample Compartment by aligning the {wo alignment pins (parallel with the light beam} with the two holes on the bottom of the x-y base. Tighten the rear screw. It may not be necessary to feplace the front screw since the alignment pins will properly place the base. Adjusting the Micro-Cuvette Holder Step 8 Step? Step 10 Please tum on the power to the instrument if the unit is off. After self- Calibration, the spectrophotometer will stop at 546 nm (green light) Place your micro-cell cuvette into the cuvette holder. The light beam should be in the center of the cuvette. If the green light is centered, you are ready fo make your measurement. If the green light is not centered on the cuvette, please proceed to Step 10, You will need a basic screwdriver for the y-axis adjustment. The adjustment screw is located in the front-center of the holder (Figure-18). = Ifthe light is too high (centered on cuvette, but not optimizing minimum volume}, tum the adjustment screw counterclockwise until centered. ‘=> Ifthe light is too low (centered on cuvette, but light beam is focused below window), turn the adjustment screw clockwise until centered. 32simple way of doing this is to monitor the transmittance reading on the display while adjusting the screw. The best position is that you get maximum transmitiance reading on the cisplay. This mean that maximum light beam (energy) passes through the cuvette. Step 11 IF the light is left or right of the center of the cuvette, then the x-axis will need adjusiment. For the x-axis adjustment, tum the white adjustment knob. The white adjustment knob is located at the front-ight of the holder (Figure-19). => Remove the Front Panel (Figure-16). > Ifthe light is to the back of the instrument, tun the knob clockwise until centered. ‘= Ifthe light is towards the front of the instrument, tun the knob counterclockwise until centered. Once again the best way is to monitor the transmittance reading on the display while adjusting the knob. Tum the knob clockwise or counterclockwise until you get the maximum transmittance reading ‘on the display. This indicates that light beam is centered fo the cuvette window to the maximum (energy) Metta Fett _® shart Figure-18 — Micro-Cuvette Holder Front View eben ay <—_——ewvette eawie Gude cadusinert Ikra Mico-cel hie ightaide Figure-19 Micro-Cuvette Holder Right-side View (Note: Light beam guide is eliminated from latest design change} 33Peltier/Flowcell Accessory Installation Guide (S-2100-109P) Step | ‘Check all items from the accessory package and make sure they are complete (refer to Figure-14 for $-2100-109P): ‘> Peltier/Flowcell Controller with peristaltic pump 1 unit Cuvette Holder with Peltier base and Flow-thru Panel with tubing 1 set Installation Guide lpe (flowcell is required and flowcelt is not included in $-2100-109P package) Installation preparation and Installation procedures (too! needed: standard Philips head screwdriver) Step 2 Remove the 4-position Cuvette Holder from the Sample Compartment: Open the Sample Compartment cover and remove any cuvettes or any breakable items. Push in the Cuvette Holder Control Knob (black knob} all the way back (First position Cuvette Holder into light beam). Step 3 Unscrew the Cuvetie Holder rod by turing the knob counterclockwise until removed from holder. Genily slide the guide rod out of the sample compartment. Step 4 Looking over the Sample Compartment, remove the front screw (Figure-15). Step 5 To remove the back screw, place your hand ON TOP of the 4-position Cuvette Holder and gently slide towards the front. The back screw is then exposed and can be removed. Step 6 Remove the front panel by sliding the panel up and out (Figure-16). Step 7 Insert Cuvette Holder with Peltier Base into the Sample Compartment until the base is engaged into two hex-head guiding “pin”. Lock the base with the Philips type screws, Step 8 Align the Cuvette Holder so that maximum light will pass through the Cuvetie Holder. This can be achieved by adjusting the Cuvette Holder horizontally ‘and vertically with two setting "knobs" AAj Horizontal setting: There is one knob located in front-right of the Cuvette Holder base for horizontal (backward or forward) adjustment. Tuming this knob clockwise will "push" the Cuvette Holder backward and counterclockwise will “push” the Cuvette Holder toward you, Tum on the spectrophotometer and MONITOR the transmittance reading on the display. Locate the maximum transmittance reading on the display by tuming the knob and this should be the best horizontal Cuvette Holder setting. BB) Vertical setting: There is a long crew on top right behind the Cuvette Holder for vertical position adjustment. Tum this screw clockwise will raise the 34Step 9 Step 10 Step 11 Step 12 Step 13 Cuvette Holder and vise visa. Locate the maximum transmittance reading nd it should be the best vertical Cuvette Holder setting (Generally speaking the Cuvette Holder should be almost at its lowest position. The Z-dimension is 15mm) linor adjustment may be required if micro flowcell is used after such flowcell is installed. Place Flowcell into the Cuvetie Holder. Be sure to align the windows of the fiowcell with the horizontal light path of the instrument. Insert Flow-thru Panel for Peltier/Flowcell Accessory in place of removed panel. Connect Peltier/Flowcell Controller to the Outlet Tube Connector (Figure-20). Flowitau Perel for Peltir/Floweel Access Start Button Outet Connecter AS22Ceble Irie Correctx for Petier [Black tube) Figure -20 _ Flow-thru Panel for Peltier/Flowcell Accessory ‘Connect the 9-PIN Cable from the Flow-Thru Panel to the back of the Peltier/Flowcell Controller (Figure-21). Back Vw ol Poti Fawcel Contr Power Sitch [| Power Cort = ‘Outlet AS-232 Plug for Flowthru Panel Figure -21 Back View of Peltier/Fiowcell Controlier Connect the tubing from the outlet connector of the Flow-Thru Panel fo the Peltier/Flowcell Controller (Figure-22). Push the tubing into one of the two. locking positions on both the Front and Back side of the Peristaltic Pump. 35Front Back ToWatte. Pelin/Flowcel Contes Side View Figure -22 _Peliier/Flowcell Controller Side View Step 14 The external components are ready. Now, with tubing supplied by either the manufacturer or the user, connect the flowcell to the Flow-Thru Panel. Two different tubing will be needed inside the Sample Compartment: inlet tubing: from inlet connector (pipe) to the flowcell. Depend on the type of flowcell; the inlet tubing may have o cap with thread. The inlet tubing is usually smatler in diameter. Outlet tubing: from the flowcell to the outlet connector. This tubing is usually ‘the same size as the tubing from the outlet connector to the peristaltic pump. The length should be no less than 10 cm for each section of tubing. The Paltier/Flowcell Accessory is now ready for testing (Figure-23). Inet (pane!) Sample (even) Pam Waste Flowcell Figure-23_ The Liquid Flow Chart USING THE PELTIER/FLOWCELL ACCESSORY Step 15 Tumon the spectrophotometer (if power is off) and Peltier/Flowcell Accessory. Select the temperature (25, 30, or 37°C) on the Controller. To do this, push the Mode bution on the Controller so the temperature light is iluminated. Select the temperature using the "up" and "down" buttons (Figure-24). 36Peticr/Fowcel Accessory Temperature Indeatar Mode ® Button @ Flowcel Indicala’ Rediout Figure-24 _Pelitier/Flowcell Accessory Front View Step 16 Press the Mode button on the Controller. The number showing is the number of seconds the peristaltic pump on the flowcell system will operate. To adjust this, use the “up" and "down" buttons. PLEASE NOTE: Since sample size and desired volume varies from sample to sample, the user must experiment to find the optimum pump time. Ifless volume to be used is desired, then decrease the Flowcell (pump) time. If more volume is needed, then increase the time, Step 17 To start the flowcell (pump), press the red button on the Flow-Thru panel on ihe front of the instrument. Repeat as desired. NOTE: IT IS VERY IMPORTANT TO KEEP THE FLOWCELL SYSTEM CLEAN. TO ENSURE A LONGERLIFETIME FOR THE FLOWCELL, PUMP WATER INTO THE SYSTEM WHEN NOT IN USE (I.E. OVERNIG 372100 SERIES SCANNING SPECTROPHOTOMETER SOFTWARE USER’S MANUALFrederiksen® so oication Software Table of Contents Minimum Computer Requirements. Software Installation... Loading Software to ‘Computer. Connecting Computer to Spectrophotometer, Uninstall of FREDERIKSEN 2100. Installation Probiem.. Main Screen Display. Data Screen. Test Status Display. Test Screen, Basic Operation. Absorbance, %Transmittance, Concentration. Main Screen of A/ZTrans/Conc... Absorbance/%Transmittance Mode. Concentration/Standard Mode... Concentration/Factor Mode... Standard Curve... Main Screen of Standard Curve Kinetics... Main Screen of Kinetics Scanni Main Screen of Scannini Buttons of Scanning. Scan Sample... Data import to Microsoft Excel®...Froderikson® a oication Software introduction The FREDERIKSEN® Application Software—FREDERIKSEN 2100 has been designed to operate with FREDERIKSEN® Spectrophotometer Model 2100 nd 2100UV. The software runs on a PC (personal computer) with Windows® 98 2r¢ edition/Windows® ME, or Windows® 2000 Professional, or Windows? XP operating systems. Your FREDERIKSEN® Application Software package includes: * One CD containing the software (FREDERIKSEN 2100} * Software User's Manual * A6' null modem connection cable with 9-pin and 25-pin female connector's on both ends. The FREDERIKSEN® Application Software performs the following methods for analysis: Absorbance/ZTransmittance/Concentration: measure the Absorbance, %Transmittance, Concentration/Standard, or Concentration/Factor at a single wavelength within the range of 325~1000 nm (Model 2100) or 200~1000 nm (Model 2100UV}. Standard Curve: create a calibration curve (choice of 4 curve fits) with up to 8 standard solutions at a single wavelength to determine concentrations of unknown samples. Kinetics (Absorbance vs. Time Kinetics}: measure a sample's absorbance change over a selected period of time, store the test results in data table, and display the results graphically. Scanning (Absorbance/Transmittance vs. Wavelength): permit the operator to scan at interval of 1 nm at any wavelength range of 325 (200) ~1000 nm featuring zoom and peak/valley pick. Minimum Computer Requirements To properly install and operate the enclosed software, it is required to have the following minimum computer configuration: PC with: * 16MB RAM * Pentium or faster processor « 10.MB of free space on memory « VGA Color MonitorFrederiksen® 4 oication Software * PS/2 mouse and keyboard NOTE: The FREDERIKSEN® Application Software provided will NOT function with a ‘Macintosh/Apple or Linux computer. Se lation Loading Software to Computer To install the software, please close any open programs and disconnect from the Internet if oniine, then follow the instructions below. Step 1: Insert FREDERIKSEN® Application Software CD into the CD drive of your PC, If the software can show the Automatic Setup Screen as Figure-1, go to Step 4. fit can not, go to Step 2. ight click Start of your PC and click Explore, locate the CD Drive labled 2100SB-2.10-T2 as Figure-2 indicates. Step 3: Click the CD Drive Label, click the setup folder, select the right folder of the operating system you are using (shown in Figure-3): © xp: Windows XP operating system © 2k: Windows 2000 professional operating system © 98me: Windows 98 2% edition and Windows Me operating system Step 4: Click setup.exe as Figure-3 shown or click the right version of the application to be installed corresponded to the operating system you are using (shown in Figure-1). Figure-4 indicates the setup screen. Read the Welcome message carefully before proceding. If everything is OK, click Next button and go to Step 5. Step 5: Type your Name and Your Company Name as Figure-5 shown, then click Next button and go to Step 6, Step 6: By clicking the Change button (Figure-6), you can open the Browse for Folder (Figure-7) window and choose the Folder where you want the software fo be installed. If the folder selection is OK, then click Next button and go to Step 7. Setp 7: Click Next button (Figure-8) to continue FREDERIKSEN 2100 installation (Figure-9 fo Figure-11). Step 8: Wait for the message stating the software Installation completed successtully (Figure-12) and click Finish bution to end the installation. The FREDERIKSEN 2100 Icon will be shown on your desktop (Figure-13). Step 2: Congratulations! You have now installed your FREDERIKSEN® Application ‘Software FREDERIKSEN 2100 in your PC for your Model 2100/2100UV Spectrophotometer. If you have installation problem, please refer to the Installation Problem of this section.Frederiksen® 4. auction Software Frederiksen 2100 Setup Figure-1 Automatic Setup Screen Fle ES Vow Fart Teds Heb me > Lsswe | rates} (I Forte shea si Pare namaste 01a spec neces. 110 Sete hfemaion snag san 7 ieee sna $00 Figure-2 Locate CD Drive ScreenFrederiksen® s oication Software Fle Edt View Farrtes Tacs el Oe ! Ps | ras | saters [Soisenabe Fotece Goaneis sere 2Q Kos Om ine 8G pogan ries 2 woos 2D ross2.01209 Boma eQsu 2 Os i ‘See Type ake Mock 691200 ipokaton —Stisvamn soe Figure-3 setup.exe Telesse Wedeme ty the installer for W/Vixt100 Application Brie A ip stronely recomended thet gon anit ll Yindons 3 Guatloning 31Gb thie seatallation EE you have any other grogrent remming pleats click Cancal sad cleat hate pragrasn before Fentartiag thie iastailer Diharviss, Lick Want to centions, aun: This a protactsd by coprrisht Lee ad intern tralian, Unenthoriead rapreduclion er listeitation of ie ey reredt in ssrare thrid and eriainal jensltfon, wad vill ‘prosecuted to the meninan extent goavibie ender Tax Figure-4 Setup ScreenFrederiksen® a oiication Software ieee User Tafermstion Tater you wier infernation ad Alice Wert to contizns Figure-5 Install Process One alee Te insta ale Hig trpe fa ware path er cick Change te [Etieow Rissteimtrce hace ~~ [ae Spice raqcived em drive: elected Figure-6 Install Process TwoFrederiksen® a. cucation Software finery ‘Install WV/¥s2100 Appication to: 31 Florey (a) BS Loca ek) | @Q 210002,105 0) & Q owirawornere) BS Loar) 1 BB So Localdk(G:) BB Local Duk GH) | BS Loca k(t) (Be Local Dick Figure-7 Install Process Three—Folder Selection | The install fen Tha feoving settings Wild be used: Iastdl Felder: ¢: ropes Files\WMTED\Gpicteephotenater ‘Shertent frAder: WIED\Spectrepheteneter Placse Lick Feat te proceed with the inntelLaties Cee CES Cea Figure-8 Install Process Four—Folder Selection IISera Scie Pil ey Plaase wait while the macertery Ber ere tattle estaiog CAtregrn Fitec\ORTDASpectrophatennterN cl? one Ce Figure-9 Install Process Five Setep TastslLation aetieae are now being performed Please eait while satep actions ere performed an your systen, Magi staring files Figure-10 Install Process SixFrodorikson® 4. ciication Software See Figure-11 Install Process Seven ‘WUWLBI00 Apication SE-2.10-T2 hes bem yo ire. ‘Mase click Finish te ent thix instar Figure-12 Install Process Eight—nstallation Success Figure-13 FREDERIKSEN 2100 Icon on the Desktop 10Frederiksen® 4. ueation Software Connecting Computer to Spectrophotometer Step 1: Remove the RS232 Connection Cable (double end) from the bag. Step 2: Locate the RS-232C port on the back of your PC. Connect the female 9-pin (small) connector (one end of the Connection Cable) to the male 9-pin of your PC and secure with the builfin screws. If your PC does not have a male 9-pin connection, use the male 25-pin connector. ONLY USE The 25-pin connector if your PC does not have a male 9-pin connector (common for older, upgraded computers). Step 3: Model 2100/2100UV Spectrophotometer has a male 9-pin RS-232C port on the back. Connect the other end of the RS232 connection cable to your Model 2100/2100UV and secure tightly. Step 4: Tum on your PC [if not already on) and your Model 2100/2100UV {if not already on, let it warm up for fifteen minutes). Step 5: Click the Start button on your PC, scroll to All Programs, FREDERIKSEN, and locate the 2100 1.0(Figure-14), and click to open it. Step 6: The Start-up Screen of FREDERIKSEN 2100 will appear as Figure-15 shown, Step 7: Click EER! a window will pop-up as Figure-16. Step 8: Select the proper port in the pop-up window, push the Connect button, and then press the ENT key on the Model 2100/2100UV pane! {Figure-16 and Figure-17). The FREDERIKSEN 2100 will initialize (Figure- 18) and go to the main screen (Figure-19 to Figure-21). Figure-14 Open the FREDERIKSEN 2100 WFroderiksen® lication Software fo OY Bh alarthile esucriald Application for UV-2100/2100 Version 1.0 Figure-15 Start-up Screen eases} Gi Searil Comm. Setup Figure-16 Port Selection eRe ees i=lc3] Please press the key on the instrument.try again! [cow Cancel Figure-17 Connect to PCFrederiksen® jg oication Software Application for UV-2100/2100 Aen) Figure-19 — Model 2100UV Main Screen DisplayFigure-20 Model 2100 Main Screen Display CAUTION: The Main Display Screen of the spectrophotometer display as Figure-19 to Figure-20 shown. The buttons on the Operation Panel of 2100 and 2100 UV (Figure-22) are functionless. Some photos used below have the Exit and Enter buttons shown in Figure-23, The Exit button has been removed and the Enter button has been changed to OK button as Figure-19 shown). Please note that there are no major differences, just follow the instructions. Figure-21_ PC CONN Screen Figure-22 2100UV Operation Panel 14Frederiksen® a. ciication Software | Figure-23 Main Screen Display Button Change Uninstall of FREDERIKSEN 2100 Click the Start button on your PC, scroll to All Programs, FREDERIKSEN, Spectrophotometer, locate the Uninstall 2100 Spectrophotometer (Figure-14), ‘and click to remove it (Figure-24 and Figure-25). Click Close button to finish the uninstall process (Figure-25). (QD) smyussettrait onmen Neeatn 8 bead eronet Figure-24 — Uninstall Process One Figure-25 Uninstall Process TwoFrederksen® 4. ication Software Installation Problem When you try fo install the software to Windows 98, Windows Me, or Windows 2000 professional operating systems using the 98me or 2k version of the installation software (Figure-3), you may get the following error messages (Figure-26 and Figure-27). Try to use the xp version of the installation software to install tne application in your Windows 98, Windows Me or Winclows 2000 professional operating systems, If you still can not install if, contact info@Frederiksen L.com or call 1-800-588-9776. a € cxmcree be otonog area | DiWNOOKESISTEN Ce] ow Figure-26 — 98me installation Error x e Carpokcrestethe folowing drectory: caymemsysence Cincy] _coet_| Figure-27 — w2k installation Error ‘Main Screen Display After your Model 2100/2100UV is connected to your PC, and FREDERIKSEN 2100 is running, your PC will show the Main Screen Display like Figure-19. The FREDERIKSEN 2100 Display Screen can be divided into three parts: Data Screen (Figure-28 Data Screen), Test Status Display (Figure-29 Test Status Display) and Test Screen (Figure-30 Test Screen). Data Screen Data Screen has two parts: * Data Screen Display area * DataTableFrederiksen® a. oi cation Software Data Screen Display area has ten Text Fields. You can input texts to four ‘of them, which have Sample Name, Operator, Filename, and Date labels at their left. The Text Fields of WL, Mode, C/Std, C/Factor, and Units, which have labels at their left, are set by Test Screen input. The Time Text Field is synchronized with the PC's clock. Data Table is the blank area under Data Display Screen. It records the text results automatically. Figure-28 Data Screen Test Status Display It shows the current status of the FREDERIKSEN 2100: * Text Field under WAVELENGTH label shows the current wavelength of Model 2100/2100UV * Tex! Field under DATA label shows the current data both in Absorbance and Transmittance Text Field under SAMPLE shows the position of the sample in the Cuvette Holder (itis the future function) * Text Field under LAMP shows the UV and VIS lamp status Figure-29 _ Test Status Display Test ScreenFrederiksen® j pication Software It has nine buttons/icon buttons: . . Application button: select the four test methods: A/%I/Conc, Standard Curve, Kinetics, and Scanning (Figure-31 Test Methods) Save as icon button: save the setup or data displayed Load icon button: open any saved file (.1st) Print icon button: print all information shown in the Data Screen and any data collected 0A button: set 0 Absorbance and 100%T 02 button: set 071 (0%T black block required in the Cuvette Holder) Goto button: set the FREDERIKSEN 2100's wavelength shown at its left. You can either clicking =o or typing the wavelength into the Test Field at the left of Goto button OK button: enter the test mode selected: Abs, %Trans, Conc./Std, or Conc./Factor Test button: click to begin test and record the test resulis You can enter Sample ID number—the numerical number from 1 to 999 or letter or both of them at the right of SAMPLE ID label after test mode is selected. Apacer | | | Son Sep: wewwinan FE | Goto [Test Mode Setup: as © stems © enesstt © come Rector tee Semeiono, Fie Figure-30 Test ScreenFrederiksen® a oucation Software © ATIC © STANDARD CURVE © KINETICS © SAMPLE SCAN Figure-31 Test Methods Basic ation Four Analytical Methods—Absorbance/%Transmittance/Concentration, Standard Curve, Absorbance vs. Time Kinetics, and Scanning are illustrated below. Absorbance, %Transmittance, Concentration The Absorbance, %Transmittance, Concentration (A/%trans/Conc) method has the following three modes of operation: * Absorbance/Zlransmittance * Concentration/Standard * Concentration/Factor Main Screen of A/%Trans/Conc At the Maln Scteen, click Application, check A/%I/C (Figure-31), and click OK button to enter the Main Screen of A/71/C (Figure-19). Type Sample Name, Operator (name), Filename, and Date into the appropriate text fields. Absorbance/%Transmittance Mode The following are the basic operation procedures. Step 1: Insert reference cuvette or nothing into the Sample Compartment and close the lid. = Step 2: Select the desired wavelength by clicking X= button on the PC screen to set or typing in the desired wavelength, click Goto button at the right of = button to set the wavelength. 19Frederiksen® 4 sacation § Step 3: Click on OA button fo blank the reference (Figure-32). Step 4: Select the test modes: Abs or %lrans, and press OK (or Enter) button (Figure-33 Test Mode Selection} Step 5: You may type in Sample ID number at the right of SAMPLE ID label after test mode is selected. The ID number can be the numerical number from 1 to 999 or letter or both. Step 6: Remove the reference ifitis there] and place your sample cuvette info the Sample Compartment, close the lid and click on TEST Button. The test results will be displayed in a spreadsheet format in the Data Table at the Data Screen. Two sample test results are shown in Figure-34 and Figure-35. Figure-32 0A BlankingFrederiksen® po oication Sofware Figure-33 Test Mode Selection Figure-34 Sample Test Results of Abs Mode 21Fraderlksen® 4. cucotion Sotiware mr || Figure-35 Sample Test Results of Trans Mode Conceniration/Standard Mode The purpose of this test is to determine the Concentration of the unknown samples by comparing the samples’ Absorbance/Transmittance to that of the standard solution. Repeat Step 1 to 3 in Absorbance/%Transmittance Mode section. Step 4A: Select the Test Mode: Cone./Std, type in the known concentration of the standard solution in the Text Field at the right of Conc./Std label, type in the unit in the Text Field at the right of the UNITS label. Step SA: Place the standard in the Sample Compartment and close the lid. Step 6A: Click the OK or Enter button. This will measure the Absorbance of the standard and set its conversion Factor for measuring the unknown samples. 2Frederiksen® s.pication Software Figure-36 Sample Test Results of Concentration/Standard Mode Step 7A: Place your sample(s} in the Sample Compartment and click the TEST button for each sample to be measured, Absorbance and the Concentration of the samples will be displayed in the Data Table (Figure-36). Concentration/Factor Made The purpose of this test is to measure the Concentration of the samples with known multiplication factor to calculate the Concentration. Repeat Step 1 to 3 in Absorbance/ZTransmittance Mode section. Step 4B: Select the Test Mode: Conc./Factor, type in the desired Factor in the Text Field at the right of Conc./Factor label, type in the unit in the Text Field at the right of the UNIT label (Figure-37 Factor Setting). Step 5B: Place the samples into the Sample Compartment, close the lid, and click the OK or Enter button. Step 6B: For each sample, be sure to place the sample in Sample Compartment and close the lid. Click the TEST Button to record results. The Absorbance and Concentration of the samples will be displayed in the Data Table (Figure-38). 23Frederiksen® 4. ication Software Figure-37 Factor Setting Figure-38 Sample Test Results of Concentration/Factor Mode Standard Curve The Standard Curve (Calibration Curve) method allows the operator to * Measure up to 8 standards 4 Calculate standard curves with 4 curve fits, including: 1, Unear Thru Zero: set the y-intercept equal to zero; therefore, the curve is forced through zero. Calculate and display the slope and Correlation Coefficient.Frederiksen® 4. ication Software 2. near Squares: Linear regression model. Calculate and display the slope, y-intercept, and Correlation Coefficient for the given standards. 3. 24 Order: second derivative of the Linear Squares model. Calculate and display the coefficients. This method is used for non- linear standard curves or curves with a Correlation Coefficient of less than 0.9. 4, Segmented: straight line. Use the standards as nodes to connect each point... No data is displayed or calculated. * Select and view existing standard curves * Calculate the Concentrations of unknown samples Main Screen of Standard Curve At the Main Screen, click Application, check Standard Curve (Figure-31), and click OK button to enter the Main Screen of Standard Curve (Figure-39). Type Sample Name, Operator (name), Filename, and Date into the appropriate text fields. Ede hoo agree HI Semple Troma: cat abe Figure-39 Main Screen of Standard Curve 25Fredoriksan® 4. ication Software Figure-40 Sample Test Results of Standard Curve The following are the basic operation procedures. Step |: Insert reference cuvette or nothing into the Sample Compartment ‘and close the lid. Step 2: Select the desired wavelength by clicking SJ button on the PC screen to set or typing in the desired wavelength, click Goto button at the right of | button to set the wavelength. lick on OA button to blank the reference. Step 3: Step 4: Under Standard Setup, at the right of SAMPLES label, click 3 button and set the number of standards (from 2 to 8) to be used. Step 5: Type the units to be displayed at the text field of UNITS label. Step 6: Place the standards in the Sample Compartment in order of lowest concentration to highest concentration. Type the concentration of the standards (e.g. 0.09 here) into the Text Field starting below C/Std label. Press Enter key on your computer keyboard or click with the mouse to move to the next cell. Repeat the same operation until all concentrations of the standards have been entered (Figure-40). Step 7: Measure the Absorbance of each standard. For each measurement, insert the standard into Sample Compartment and double click in the appropriate Absorbance cell (Abs) next fo the standard C/Std or type in the current Abs display from Test Status Display to get the Absorbance for curve drawing. Continue until all of the standards have been measured. 26Frederiksen® 4 cation Software Step 8: Press the Draw button to graph the Standard Curve (Figure-41). You may save it for future use. [be7] frorbro eres | | fee 9 — tMeaneecreaccne | aaa lf wo ag ‘oo | 4 Ee eh a | | Lar See eu om} ( ets | fe tom) | Figure-41 Standard Curve Graphic Display Your standards have now completed setup. To use the graph and measure the unknown sample concentrations, please be guided by the steps below: Step 1: Select the desired Standard Curve by clicking on one of the five buttons (Figure-42). Shown in Figure-41 is Linear Squares (Least Squares Method). Items in the equation next fo the Linear Squares title on the graph are Abs, the slope, and y-intercept as well as Correlation Coefficient, Abs = slope * C + y-Intercept Cor. Coef = Correlation Coefficient Step 2: (optional) Click Print icon button: print the graph and labels of the slope, y-intercept ond Correlation Coefficient as seen on the screen. Step 3; When ready to measure samples of unknown concentration, press the Test button to retum fo the main Standard Curve screen (Figure-39). Step 4: To measure the concentration of unknowns, place the samples in the Sample Compartment and click on Test button, located at the bottom-left portion of the screen. step 5: To save the data with the Standard Curve fit selected, cick on the Kal (Save Icon}, name the file, and click Save. 7Fi Tederiksen® 4 ication Sofware te] poate res] [P=] [= . Figure-42 The Five Function Buttons of Standard Curve Kinetics The Kinetles application has the following functions: * Setup kinetics Test Parameters * Obtain kinetics data for a sample at a single wavelength * Load and save data files for further studies ‘Main Screen of Kinetics At the Main Screen, click Application, check Kinetles (Figure-31}, and click OK button to enter the Main Screen of Kinetics. Type Sample Name, Operator (name), Filename, and Date into the appropriate text fields (Figure-43). The following are the basic operation procedures. Step 1: Insert reference cuvette or nothing into the Sample Compartment and close the lid. step 2: Select the desied wavelength by clicking === button on the PC screen to set or typing in the desired wavelength, click Goto button ot the right of = bution to set the wavelength. lick on OA button to blank the reference. jet the TEST SETUPS (Step 4~Step 8). Set the Total Run Time by typing into the Text Field on the right of the TOTAL RUN TIME label. The three blocks represent hour, minute, and second respectively. Step Step 4: 28Frederiksen® ss tcation RTA ScrEEN ° jee | Application Wt Set; —___ Wenteors BIT =} _ Goto Toe Setup: | | Mote Jem ge] ve [—] Figure-43 Kinetics Main Screen Step 5: Set INTERVAL TIME similar as Step 4. This is the time interval for which measurements will be recorded [i.e. every 10s, or every 35, etc.). Step 6: Set an INITIAL DELAY similar as Step 4. The purpose of this step is to delay the beginning of data collection. {i.e. Sample must be injected, and reaction will not begin for 20). Step 7: Set a FACTOR (multiplication factor-dilution factor) similar as Step 4. This allows for a factor to be used when calculating the Concentration of the solution, Step 8: Set the HIGH and LOW Limits of the graph similar as Step 4. This is the selection of the minimum and maximum Absorbance range for the graph of the data, Step 9: Type the unit in the Text Field at the right of the UNITS label. Place your sample in the and click the TEST button once all the TEST SETUPS have been set and you are ready to start your measurement Step 10: Click Start button to begin test (You may type the name of the Kinetics test in the text field at the right of the Name label, like “Water” of this sample test, see Figure-43). The test result is shown in Figure-45, Start button: start the Kinetics test (Figure-44) Stop button: stop the data collection at any given moment Back button: go back to the Kinetics Test Display Screen 29Frederiksen® 4 oucation Software set ra 8 | = ee Nome: et Figure-44 Three Function Buttons for Kinetics Test Figure-45 Sample Test Results of Kinetics 30Frederiksen® soication Software Scanning The Scanning application has the following functions: * Select a data scan mode (Abs. or %T) * Select from two scan speeds: high and low * Set the scale for the spectrum * Set the scanning wavelength range * Zoom function and Peak/Valley pick function * Print, save and load data files Main Screen of Scanning At the Maln Screen, click Application, check Sample Scan (Figure-31), and click OK button to enter the Man Screen of Scanning with a pop up window for Scan Setup (Figure-46 and Figure-49). [ee sae eros |] (=o 1 rs ee | er ha ee = = sw memes pa | Lee} Figure-46 Main Screen of Scanning Buttons of Scanning The Scanning function has two button groups to run the application: Ge Button Group and Scan Button Group (Figure-47 and Figure-48). @[alo als|a. Application | Figure-47_ General Button Group 31Frederiksen® 4. cation Software Scan Dat: Scan Baseline Stop Reset Figure-48 Scan Button Group The General Button Group has six buttons: @ Zoom ®: jo expand a section of the graph for easy viewing. Operation: Click the button, move the mouse to the upper left comer of the area desired to be enlarged in the graph; Click and hold the left-mouse button and move the mouse to highlight the desired area with a dotted square, then release the mouse button; Click ©. to retum to the original graph @ Peak/Valley |™: to label peaks and valleys of the graph for presentation and notes. Operation: To place the Peak Label, click the button, move the mouse to the top of the peak and left click the mouse. It will display the absorbance and wavelength. To remove the Peak label, left click the mouse on the displayed absorbance and wavelength. To place and remove the Valley label, repeat the operation above with right click the mouse. You can also input a number to get the peak and valley value for certain range of the wavelength. For example, type 5 (it is actually 5 nm) (Figure-49), click OK, move mouse to the curve at 500 nm, you will get the Peak and Valley value from 495 nm to 505 nm. @ Reset © : to retum to the original graph from Zoom operation and Peak/Valley operations. © Save: to save the test data. Operation: click the button and save the file with extension .tst. © Load: to open the saved .ist fle. Operation: click the button, browse the st file and open it with a mouse click. © Print: fo print the graph. Operation: click the button, the scanned graph will be printed on the printer connected to your PC. 32Frederiksen® 4 ication Software @ Application: to switch between the four test methods. The Scan Button Group has five buttons: @ Scan: to scan the sample and obtain the graph. Operation: after baseline scan, click the button to scan the sample. ® Data Table: to store the scanned data dynamically. Operation: click the button, the Data Table will pop up. © Scan Baseline: to set the baseline for the scan. Operation: click the button to begin baseline scan. © Stop: to stop the scan. Operation: click the button to stop any current scan. @ Reset: to reset the scan parameters. Operation: click the button to reset the scan parameters at the end of each scan or any time you stop the scan. Please Input The Search Width! Lo] Figure-49 Peak and Valley Search within Certain Wavelength Range Scan Sample Before scan, you must setup the following scan parameters: 1. Scan Mode 2. Scan Scale 3. Scan Wavelength Range The Scan Interval is preset at 1 nm. You can not change it. The following are the basic operation procedures. Step 1: insert reference cuvette into the Sample Compartment and close the lid. Step 2: At the Main Screen of Scanning, select Scan Mode (Abs or %Irans) and Scan Speed at the pop up Scan Setup window (Figure-50). Step 3: Set the Scan Scale. Abs is 0 fo 2.5A, %lransis 0 to 125. elect the Scan Wavelength Range. input Start WL and End WL at the ‘appropriate test fields, then click OK button. 33Frederiksen® ne, a ‘Sean Mode Scan Saeed: ae | © tow © str igh semtins [3] sentra eriareenaiomy GS eansetooton fT— veuaeiin, Fn ox Rewet Figure-50 Scan Parameter Setup Step 5: Baseline Scan, Click Scan Baseline button at the Scan Button Group tool bar. The Scan Baseline window will pop up. Retype the Start and End wavelength you just input at Step 4 into the appropriate text fields to reconfirm the wavelength range selection. Click Scan button to start the Baseline Scan process (Figure-51). Warning: Enter different Start WL or End WL may cause malfunction! Figure-S1 Scan Baseline Setup 34Frederiksen® Application Software Figure-52_ Scan Baseline | Step 6: Sample Scan. After the Baseline Scan is finished (Figure-53), remove the reference and insert the sample into the sample holder. Click & bution at the Scan Button Group tool bar to start scan sample. Figure-54 to Figure-56 shows the sample scan process, the data table, and the final graph of the scanning result. — ae a - a ae Figure-53 Scan Baseline Il 35Frederikeen® ication Software Figure-54 Sample Scan! Figure-55 Sample Scan Il Figure-56 Sample Scanning Result 36Frederiksen® pp pication Software Note: After Sample Scanning is finished, if you want fo continue to scan using current scan setting, just simply change the sample and click button to begin the new sample scanning test. If you want fo change the scan setting, please follow the instruction below, otherwise the spectrophotometer may not response: 1. Click _##_| button at the Scan Button Group. At the Sean Setup pop up window (Figure-46), select Scan Mode and Scan Speed, set Scan Scale, and select Wavelength Range, then click OK button. 2. Retype the wavelength you choose at the Scan Baseline pop up window (Figure-51), click ** at the pop up window. 3. After the screen shows ready as Figure-53, go to Step 6 of the Scan Sample procedures. To close the application, just close the test window and click EXIT, then YES. Data import to Microsoft Excel? By referring to the following steps, you can transfer any of the FREDERIKSEN? Application Soffware—FREDERIKSEN 2100 fest data to a Microsoft Excel® program: Step 1: In Microsoft Excel, click on File and click Open. Step 2: Select any saved file you wish to import, Step 3: After the Excel Text Import Wizard appears, select Delimited, select the row from which you want the import to start, and click on the Next button as shown in Figure-57. Step 4: Uncheck the Tab delimiter and select Comma delimiter as shown in Figure-58, then click the Next button. Step 5: Click the Finish button and the test data will be imported into your Excel spreadsheet. Here, further calculations can be performed from the “raw” data collected. Step 6: Save the imported file under a DIFFERENT FILE Name if you stil want to keep and open the original FREDERIKSEN® data file in FREDERIKSEN 2100. Otherwise, the original FREDERIKSEN® data file (.tst fle) will be modified by the Excel® format during importing and the modified file cannot be opened from FREDERIKSEN 2100. 37Frederiksen® rederikson® 4 nication Software Figure-57 Excel Text import Wizard Figure-58 — Excel Text 38