Dear Editor,

We thank the reviewers for their valuable feedback. Below we listed the modifications we have done in
accordance to the suggestions.

In this paper, the presence or absence of amyloidosis is an important point, and “how to
diagnose amyloidosis” is necessary.

This is added to the methods section.

The data in Table 2 presented by the authors does not show a study between A(+)FMF(+)and
A(+)FMF(-)in CRP level and needs to be added. If there are significant difference above two groups,
FMF with amyloidosis has a potentially higher inflammatory response even in the absence of attack,
resulting that TREM-1 is enhancing inflammation.

The difference is not significant that is why we did not add the information. We edited the table to be
more clear.

Moreover, high levels of circulating sTREM-1 correlate with Visceral Leishmaniasis disease
severity and other protozoa diseases.

We added this paper to the introduction.

Lack to talk about statistical analysis and companies for biochemical methods.

The methods section is edited to include the statistical analysis and the companies of the
biochemical methods.

I would find it interesting if I could have a group in which the patients were in the attack
period. Even to show if the sTREM-1 is actually involved

This was shown in the study by Godlier et al. That is why we decided to approach the topic at hand
from a different aspect.

I suggest showing the results in the table better. For example, including the value of p
in the table for better visualization and understanding. The comparisons below of table are not
cool.

Table 2 is reformatted.

Is this information of the table 2?

We divided the patients into 2 with preserved and decreased renal functions to eliminate kidney
function’s potential being a confounding factor. This is new information and not on Table 2.

Here is unclear the n of the patients, I think that all patients were included, Is it? It is
need a careful analysis in layout and legend of the figure 1.

This information is based on all of the patients either with FMF (independent of Amyloidosis)
or with Amyloidosis (independent of FMF). Thank you for this feedback, we clarified it in the
text.
 I recommend analyze the r of correlation sTREM-1with CRP in the FMF patients, It
is not possible that curve in figure 1 D is the r=0,845.

Figure 1 is edited to have correct information.

This group of patients with amyloidosis was composed of older people. Was the
increase in sTREM-1 associated with amyloidosis, or because these patients had a high
creatinine level?

This part of this discussion is edited to include more information from the study to be more
clear.