18/24/2020 7) Crystals of NONI were grown in small droplets containing specific ells, buffer and pH, Sale the solution canfiions tha yielded
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7) Crystals of NDM-1 were grown in small droplets containing specific salts, buffer and pH.
State the solution conditions that yielded the crystals of NDM-1. (2 pts)
8) What were the unit cell dimensions (lengths and angles) of the NDM-1 crystals? (2 pts)
9) Was the phase problem solved by MR, MIR, MAD? Why was this method selected? (2 pts)
10) Is the NDM-1 enzyme a monomer in solution, or a dimer? Briefly describe the
experimental evidence that supports your answer.
Answer in your own words, in one or two sentences that could easily be understood by an
undergraduate student who has had one semester of biochemistry. (4 pts)
11) One amino acid in the NDM-1 structure has torsion angles that are in an unfavorable
region of the Ramachandran plot (see page 1485).
(a) What’s a Ramachandran plot? (2 pts)
(b) Which specific amino acid in NDM-1 is in the unfavorable region of the Ramachandran
plot? What is unique about the environment of this aspartate? (2 pts)
Answer using sentences that could be easily understood by an undergraduate student in
biochemistry.
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7) Crystals of NDM - I were grown in small droplets containing specific
salts, buffer and PH
State the solution conditions that yielded the crystals of NDM - 1. (2
pts)
8) What were the unit cell dimensions (lengths and angles ) of the
NDM - 1 crystals ? (2 pts )
hitps:iwwn-coursehero.com/tutors-problems/Biochemistry/16453159-Please-referio-he-sllachment-to-answer-tis-question-This-question/ 1“1872472020 7) Crystals of NOMI were grown in smal droplets containing specifics
butfer and pH. Stale the solution conditions that yilded
COVID-19: We are in this together. Access free textbook solutions and ask 5 free questions to
expert tutors 24/7,
rina stuay resources w
be understood by an
undergraduate student who has had one semester of biochemistry. (4
pts)
11) One amino acid in the NDM - | structure has torsion angles that are
in an unfavorable
region of the Ramachandran plot ( see page 1485 ).
(a) What's a Ramachandran plot ? ( 2 pts )
(40) Which specific amino acid in NDM - lis in the unfavorable region
of the Ramachandran
plot ? What is unique about the environment of this aspartate ? (2 pts)
Answer using sentences that could be easily understood by an
undergraduate student in
biochemistry
Subject: Biochemistry, Biology, Science Report
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she alachment-to-answerthis-queston-This-question! is18/24/2020 7) Crystals of NONI were grown in small droplets containing specific ells, buffer and pH, Sale the soltion canfiions tha yielded
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